AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0380720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0380720 MtrunA17_Chr3g0134411 81.769 373 16 2 1 322 1 372 0.0 611
Msa0380720 MtrunA17_Chr3g0134401 67.778 360 45 4 1 309 1 340 5.11e-169 473
Msa0380720 MtrunA17_Chr3g0134421 57.778 360 93 4 1 309 1 352 6.00e-133 382
Msa0380720 MtrunA17_Chr1g0152871 44.152 342 120 6 24 307 30 358 2.82e-88 268
Msa0380720 MtrunA17_Chr3g0134441 44.011 359 117 6 1 306 2 329 7.17e-83 253
Msa0380720 MtrunA17_Chr3g0134431 43.750 240 73 3 96 289 13 236 2.18e-55 181
Msa0380720 MtrunA17_Chr3g0138431 36.029 272 121 2 79 299 200 469 8.49e-50 172
Msa0380720 MtrunA17_Chr3g0125051 35.662 272 122 2 87 307 235 504 2.97e-49 171
Msa0380720 MtrunA17_Chr1g0148681 33.127 323 141 5 46 299 96 412 2.74e-48 167
Msa0380720 MtrunA17_Chr2g0332171 36.014 286 127 4 74 306 203 485 4.32e-48 168
Msa0380720 MtrunA17_Chr3g0125041 34.323 303 132 4 57 306 220 508 7.16e-48 167
Msa0380720 MtrunA17_Chr2g0332181 34.050 279 130 3 79 306 154 429 4.02e-46 161
Msa0380720 MtrunA17_Chr7g0255771 28.470 281 142 10 72 298 177 452 3.94e-24 102
Msa0380720 MtrunA17_Chr8g0370671 28.906 256 132 7 84 293 168 419 6.61e-24 101
Msa0380720 MtrunA17_Chr5g0441371 25.600 250 133 5 83 284 156 400 2.58e-22 96.7
Msa0380720 MtrunA17_Chr5g0406741 27.734 256 125 5 89 293 169 415 1.14e-21 94.7
Msa0380720 MtrunA17_Chr8g0357001 29.114 237 103 9 64 253 471 689 3.78e-20 91.3
Msa0380720 MtrunA17_Chr3g0102621 24.303 251 120 10 76 265 5 246 7.17e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0380720 AT5G43080.1 42.017 357 140 6 3 308 12 352 1.18e-92 280
Msa0380720 AT5G43080.2 41.193 352 145 6 3 308 12 347 9.46e-90 272
Msa0380720 AT1G47230.1 40.608 362 149 6 3 308 11 362 7.93e-87 265
Msa0380720 AT1G47230.2 40.496 363 149 6 3 308 11 363 1.25e-86 265
Msa0380720 AT1G47210.2 40.164 366 152 4 3 308 11 369 1.63e-85 262
Msa0380720 AT1G47220.1 44.523 283 106 1 77 308 41 323 1.46e-76 238
Msa0380720 AT5G11300.1 37.234 282 112 3 67 297 159 426 3.70e-51 175
Msa0380720 AT1G80370.1 36.842 266 114 3 92 306 197 459 3.10e-49 171
Msa0380720 AT1G44110.1 35.842 279 126 3 79 306 181 457 2.88e-47 165
Msa0380720 AT1G15570.1 36.466 266 115 3 92 306 188 450 8.98e-47 164
Msa0380720 AT5G25380.3 33.692 279 131 3 79 306 160 435 2.53e-44 157
Msa0380720 AT5G25380.1 33.692 279 131 3 79 306 160 435 2.53e-44 157
Msa0380720 AT5G25380.4 33.692 279 131 3 79 306 166 441 3.91e-44 157
Msa0380720 AT1G77390.1 32.192 292 133 3 66 306 164 441 3.29e-41 149
Msa0380720 AT5G25380.2 32.443 262 124 2 79 289 160 419 9.15e-41 147
Msa0380720 AT1G47210.1 42.328 189 93 3 3 182 11 192 1.47e-39 138
Msa0380720 AT5G11300.2 36.437 247 83 4 67 253 159 391 1.95e-39 144
Msa0380720 AT2G26760.1 27.723 303 152 6 43 297 95 378 6.06e-25 103
Msa0380720 AT1G76310.1 27.174 276 150 6 67 295 152 423 1.38e-23 100
Msa0380720 AT5G06150.1 28.519 270 141 8 71 293 160 424 2.00e-23 100
Msa0380720 AT4G37490.1 29.961 257 123 9 92 296 165 416 7.64e-23 98.6
Msa0380720 AT3G11520.1 29.961 257 129 7 83 293 150 401 8.46e-23 98.2
Msa0380720 AT1G16330.1 26.259 278 136 8 58 284 370 629 4.44e-22 97.4
Msa0380720 AT1G20610.1 26.230 244 130 5 92 289 177 416 5.25e-22 96.3
Msa0380720 AT3G11520.2 31.304 230 107 7 83 266 150 374 4.39e-21 93.2
Msa0380720 AT1G76310.2 27.500 240 123 6 66 258 151 386 9.74e-20 89.4
Msa0380720 AT4G35620.1 29.762 252 121 8 92 294 172 416 1.41e-17 83.2
Msa0380720 AT2G17620.1 30.451 266 128 8 92 310 171 426 5.82e-17 81.6
Msa0380720 AT1G76310.4 35.833 120 73 3 66 184 151 267 3.02e-16 78.6
Msa0380720 AT5G06150.2 38.261 115 66 4 71 184 160 270 4.40e-16 78.2
Msa0380720 AT1G76310.3 36.134 119 72 3 67 184 152 267 5.39e-16 79.0
Msa0380720 AT4G35620.2 31.553 206 86 7 92 249 172 370 1.06e-15 77.4

Find 100 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACGTTTGGCATTGATTT+TGG 0.171036 3_1:-82660019 None:intergenic
ATGAGAAAGTGATGAAAATT+AGG 0.184044 3_1:-82659731 None:intergenic
TAAAACAATCCTCTGTCTTT+TGG 0.193245 3_1:-82659857 None:intergenic
CATGCTGCTGGACTGGATTT+TGG 0.200580 3_1:+82662081 Msa0380720:three_prime_UTR
GTGGTGATACTTTCAAATTT+TGG 0.203595 3_1:+82662020 Msa0380720:three_prime_UTR
ATGAACCGGCTTATTCAAAT+TGG 0.248642 3_1:-82659995 None:intergenic
TACTCATCTCTGTGCATATT+TGG 0.249159 3_1:+82662440 Msa0380720:three_prime_UTR
TCCCTTTCAGGAGGTTTATT+GGG 0.251091 3_1:+82661293 Msa0380720:intron
CAGATATTCAAACTGCAAAT+TGG 0.300324 3_1:-82661430 None:intergenic
TTCCCTTTCAGGAGGTTTAT+TGG 0.306403 3_1:+82661292 Msa0380720:intron
TCTTGCTGACTTAAGCTTGT+TGG 0.319462 3_1:+82661461 Msa0380720:CDS
AAATATGAATTTGTTTATCA+TGG 0.331512 3_1:-82662236 None:intergenic
TTACAGACTCAACATGATTA+TGG 0.332086 3_1:-82662479 None:intergenic
GTGGCTGAATTCTTGAGGTT+AGG 0.334651 3_1:-82659952 None:intergenic
GATAAGTGTGTTTGTTATCA+TGG 0.348037 3_1:-82662516 None:intergenic
TGGCTGAATTCTTGAGGTTA+GGG 0.348228 3_1:-82659951 None:intergenic
CTTATTCAAATTGGAATCAA+TGG 0.369939 3_1:-82659986 None:intergenic
ATATATAATAATCCCATTAC+TGG 0.374369 3_1:+82662105 Msa0380720:three_prime_UTR
TTCATTGCTGACCAATATAA+AGG 0.375548 3_1:+82661978 Msa0380720:exon
TGAGAAAGTGATGAAAATTA+GGG 0.377551 3_1:-82659730 None:intergenic
ACTGACAGATGAAGCGTCTC+TGG 0.379014 3_1:-82660329 None:intergenic
TGAGAGGGACATTGGTTGAT+TGG 0.379637 3_1:+82660273 Msa0380720:CDS
TCCGTCCCTGTGTGAATGCT+TGG 0.381462 3_1:+82661663 Msa0380720:CDS
CTCCATTGTGCGAAGATAAT+CGG 0.397103 3_1:-82660079 None:intergenic
ATGGCCACATGCTGCTGGAC+TGG 0.398568 3_1:+82662074 Msa0380720:three_prime_UTR
AATCTGTTGATTATCACGTT+TGG 0.400606 3_1:-82660031 None:intergenic
TCAATTGTTTATGATTTCTA+TGG 0.402405 3_1:+82662313 Msa0380720:three_prime_UTR
AACTTGTGGTGGTTGTTGAT+TGG 0.402452 3_1:-82659827 None:intergenic
TTCTTGCCAGATTTATTATC+CGG 0.409058 3_1:+82661535 Msa0380720:CDS
TGGTTGTTGATTGGTGTTGT+TGG 0.411534 3_1:-82659818 None:intergenic
TCCAGGCCGGATAATAAATC+TGG 0.432284 3_1:-82661541 None:intergenic
TACTGATGGCCACATGCTGC+TGG 0.445267 3_1:+82662069 Msa0380720:three_prime_UTR
TGAATTTGTTTATCATGGAC+AGG 0.446783 3_1:-82662231 None:intergenic
AAGCAGTCAGCACCAGTAAT+GGG 0.449520 3_1:-82662117 None:intergenic
ATGTAGTACAATTAACTTTG+TGG 0.450273 3_1:+82661949 Msa0380720:CDS
GGTGACATCGGAAAGGGTTA+AGG 0.453180 3_1:-82662550 None:intergenic
TTATCTGCTTGTACTGCTAA+TGG 0.453475 3_1:+82662155 Msa0380720:three_prime_UTR
TTTGTCACGAACAGCTTTGA+AGG 0.454235 3_1:-82661758 None:intergenic
AAGTAATGATTCGGTATCTC+TGG 0.454323 3_1:-82661914 None:intergenic
TCCGGCCTGGAGTACATCCT+TGG 0.454728 3_1:+82661553 Msa0380720:CDS
TCTTCTTCAAAGTAATGATT+CGG 0.456454 3_1:-82661923 None:intergenic
GCCATCTGTTGTCGCTGCAT+CGG 0.466961 3_1:+82661506 Msa0380720:CDS
TGCTGACAGGTTCAATAGAT+AGG 0.467250 3_1:-82660370 None:intergenic
GCCAGATTTATTATCCGGCC+TGG 0.470239 3_1:+82661540 Msa0380720:CDS
TCAACAACCACCACAAGTTA+TGG 0.471210 3_1:+82659831 Msa0380720:CDS
GATGAGTAGTTCAACTCTAA+AGG 0.472561 3_1:-82662425 None:intergenic
ATTGGTTGATTGGTTAGTTG+AGG 0.479448 3_1:+82660283 Msa0380720:CDS
CAAGCAGTCAGCACCAGTAA+TGG 0.481901 3_1:-82662118 None:intergenic
GTTGTTGGCTCTCTTCTTTG+AGG 0.481908 3_1:-82659803 None:intergenic
TGATTCCAATTTGAATAAGC+CGG 0.489108 3_1:+82659990 Msa0380720:CDS
GTATCTCTGGCGATGAAGGC+AGG 0.491666 3_1:-82661901 None:intergenic
AGTGTGTTTGTTATCATGGT+AGG 0.492704 3_1:-82662512 None:intergenic
GTTAATACAGCCTATGCTCT+GGG 0.492962 3_1:+82662206 Msa0380720:three_prime_UTR
TGTTAATACAGCCTATGCTC+TGG 0.494159 3_1:+82662205 Msa0380720:three_prime_UTR
TTCGGTATCTCTGGCGATGA+AGG 0.497141 3_1:-82661905 None:intergenic
CCGTCCCTGTGTGAATGCTT+GGG 0.501028 3_1:+82661664 Msa0380720:CDS
CTACATTGGAGTTTCTGAGT+TGG 0.501899 3_1:-82659922 None:intergenic
ATGATTTGTATATGTCAAGA+AGG 0.502659 3_1:+82661728 Msa0380720:CDS
TGCATATTTGGTGATACAAT+GGG 0.502898 3_1:+82662452 Msa0380720:three_prime_UTR
AATTGGAATCAATGGAAGGA+AGG 0.516712 3_1:-82659978 None:intergenic
AGATCTAAGCTTCAGCTGCT+TGG 0.517791 3_1:+82660392 Msa0380720:CDS
CCGGCCTGGAGTACATCCTT+GGG 0.517846 3_1:+82661554 Msa0380720:CDS
GGTTATACTGCAATAGTTAG+TGG 0.518947 3_1:+82662278 Msa0380720:three_prime_UTR
CTTCACTACACTCTGAAAGA+TGG 0.519308 3_1:+82659771 Msa0380720:exon
TTGTATATGTCAAGAAGGGC+AGG 0.519915 3_1:+82661733 Msa0380720:CDS
TTCAAATTGGAATCAATGGA+AGG 0.530972 3_1:-82659982 None:intergenic
ATCAAACATGAGAGGGACAT+TGG 0.531177 3_1:+82660265 Msa0380720:CDS
CAACTCAGAAACTCCAATGT+AGG 0.541717 3_1:+82659923 Msa0380720:CDS
GTTATACTGCAATAGTTAGT+GGG 0.543346 3_1:+82662279 Msa0380720:three_prime_UTR
GTGCATATTTGGTGATACAA+TGG 0.543995 3_1:+82662451 Msa0380720:three_prime_UTR
TCGGAGATATCAGATACATA+AGG 0.545360 3_1:-82660060 None:intergenic
ACCGATGCAGCGACAACAGA+TGG 0.555704 3_1:-82661507 None:intergenic
TCTGGAACAGTTAAAATGTG+TGG 0.557657 3_1:+82661874 Msa0380720:intron
ATTGGAATCAATGGAAGGAA+GGG 0.563267 3_1:-82659977 None:intergenic
GGGTTGTGGCTGAATTCTTG+AGG 0.568858 3_1:-82659957 None:intergenic
TGGAGAGGTGACATCGGAAA+GGG 0.570202 3_1:-82662556 None:intergenic
CCCAAGGATGTACTCCAGGC+CGG 0.572797 3_1:-82661554 None:intergenic
TGAAGCTTAGATCTGCTGAC+AGG 0.573991 3_1:-82660383 None:intergenic
TTTACCCAAGGATGTACTCC+AGG 0.575735 3_1:-82661558 None:intergenic
CTCCGATTATCTTCGCACAA+TGG 0.576963 3_1:+82660077 Msa0380720:CDS
TTGGGAACCATAACTTGTGG+TGG 0.584716 3_1:-82659838 None:intergenic
ATCAATGGAAGGAAGGGTTG+TGG 0.587426 3_1:-82659971 None:intergenic
TGGACAGGACCCCAGAGCAT+AGG 0.594229 3_1:-82662216 None:intergenic
CTGGAGAGGTGACATCGGAA+AGG 0.595104 3_1:-82662557 None:intergenic
TGATTTGTATATGTCAAGAA+GGG 0.596859 3_1:+82661729 Msa0380720:CDS
TTCTCAAGATAACCAACCAT+TGG 0.599787 3_1:-82660224 None:intergenic
CGATTATCTTCGCACAATGG+AGG 0.617827 3_1:+82660080 Msa0380720:CDS
TATCTTGAGAATGTTCAAAG+AGG 0.624395 3_1:+82660236 Msa0380720:CDS
GCATTACATCAAACATGAGA+GGG 0.627034 3_1:+82660258 Msa0380720:CDS
GGCATTACATCAAACATGAG+AGG 0.629142 3_1:+82660257 Msa0380720:CDS
GATTGCTTGTGAGAATCGAA+AGG 0.629577 3_1:+82661315 Msa0380720:CDS
CCCAAGCATTCACACAGGGA+CGG 0.632875 3_1:-82661664 None:intergenic
AAATCCAGTCCAGCAGCATG+TGG 0.653607 3_1:-82662078 None:intergenic
AAGAGATATATTATAGCACA+TGG 0.656766 3_1:+82662369 Msa0380720:three_prime_UTR
TTAATACAGCCTATGCTCTG+GGG 0.658962 3_1:+82662207 Msa0380720:three_prime_UTR
TGACAAATACAAGCAGAACA+AGG 0.662422 3_1:+82661774 Msa0380720:CDS
TTATGGTTCCCAAAAGACAG+AGG 0.675551 3_1:+82659848 Msa0380720:CDS
GTAACCCAAGCATTCACACA+GGG 0.699025 3_1:-82661668 None:intergenic
TGTAACCCAAGCATTCACAC+AGG 0.705344 3_1:-82661669 None:intergenic
TGAATGCTTGGGTTACACGT+CGG 0.724505 3_1:+82661675 Msa0380720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAACAAATTCATATTTTT+TGG + chr3_1:82662241-82662260 Msa0380720:three_prime_UTR 10.0%
!!! AAATATGAATTTGTTTATCA+TGG - chr3_1:82662239-82662258 None:intergenic 15.0%
!! ATAACAATTAATCAAGAAGA+GGG - chr3_1:82660110-82660129 None:intergenic 20.0%
!! AATAACAATTAATCAAGAAG+AGG - chr3_1:82660111-82660130 None:intergenic 20.0%
!!! TGTTATTTTTACAAAGATCT+AGG + chr3_1:82660483-82660502 Msa0380720:intron 20.0%
!!! TATTTTTGTATAAGCTATCT+TGG + chr3_1:82660615-82660634 Msa0380720:intron 20.0%
!! TCAAATTCATTAATCTCTAA+GGG - chr3_1:82660767-82660786 None:intergenic 20.0%
!! ATCAAATTCATTAATCTCTA+AGG - chr3_1:82660768-82660787 None:intergenic 20.0%
!! TAGAGATTAATGAATTTGAT+AGG + chr3_1:82660768-82660787 Msa0380720:intron 20.0%
!! AGAGATTAATGAATTTGATA+GGG + chr3_1:82660769-82660788 Msa0380720:intron 20.0%
!! ATAGATGATAGAACATTAAT+GGG + chr3_1:82660797-82660816 Msa0380720:intron 20.0%
!! TAAGAAATGTTATGACATTA+GGG - chr3_1:82661180-82661199 None:intergenic 20.0%
!! TTAAGAAATGTTATGACATT+AGG - chr3_1:82661181-82661200 None:intergenic 20.0%
!! ATATATAATAATCCCATTAC+TGG + chr3_1:82662105-82662124 Msa0380720:three_prime_UTR 20.0%
!! TCAATTGTTTATGATTTCTA+TGG + chr3_1:82662313-82662332 Msa0380720:three_prime_UTR 20.0%
! CTTATTCAAATTGGAATCAA+TGG - chr3_1:82659989-82660008 None:intergenic 25.0%
!!! CATTGTTCACATGTTATTTT+GGG - chr3_1:82660659-82660678 None:intergenic 25.0%
!!! ACATTGTTCACATGTTATTT+TGG - chr3_1:82660660-82660679 None:intergenic 25.0%
! GATAGATGATAGAACATTAA+TGG + chr3_1:82660796-82660815 Msa0380720:intron 25.0%
! TAGATGATAGAACATTAATG+GGG + chr3_1:82660798-82660817 Msa0380720:intron 25.0%
! ACTTTCTGCATATTTAAAAG+TGG - chr3_1:82660921-82660940 None:intergenic 25.0%
! GTCATAACATTTCTTAAGTA+AGG + chr3_1:82661184-82661203 Msa0380720:intron 25.0%
! TAACAACAAATATTTACCCA+AGG - chr3_1:82661573-82661592 None:intergenic 25.0%
!!! TGGGTAAATATTTGTTGTTA+TGG + chr3_1:82661573-82661592 Msa0380720:intron 25.0%
!! ATGATTTGTATATGTCAAGA+AGG + chr3_1:82661728-82661747 Msa0380720:CDS 25.0%
!! TGATTTGTATATGTCAAGAA+GGG + chr3_1:82661729-82661748 Msa0380720:CDS 25.0%
! TCTTCTTCAAAGTAATGATT+CGG - chr3_1:82661926-82661945 None:intergenic 25.0%
! ATGTAGTACAATTAACTTTG+TGG + chr3_1:82661949-82661968 Msa0380720:CDS 25.0%
!! TGGTGATACTTTCAAATTTT+GGG + chr3_1:82662021-82662040 Msa0380720:three_prime_UTR 25.0%
!! TTTTGACAATCAATTACTGA+TGG + chr3_1:82662055-82662074 Msa0380720:three_prime_UTR 25.0%
! AAGAGATATATTATAGCACA+TGG + chr3_1:82662369-82662388 Msa0380720:three_prime_UTR 25.0%
! AAAACAATCCTCTGTCTTTT+GGG - chr3_1:82659859-82659878 None:intergenic 30.0%
! TAAAACAATCCTCTGTCTTT+TGG - chr3_1:82659860-82659879 None:intergenic 30.0%
! AAAAGACAGAGGATTGTTTT+AGG + chr3_1:82659859-82659878 Msa0380720:CDS 30.0%
AAAAACAGGAAGATTGAGAT+CGG - chr3_1:82659893-82659912 None:intergenic 30.0%
!! GTTGGTTGATAATCAAAAAC+AGG - chr3_1:82659907-82659926 None:intergenic 30.0%
TTCAAATTGGAATCAATGGA+AGG - chr3_1:82659985-82660004 None:intergenic 30.0%
TGATTCCAATTTGAATAAGC+CGG + chr3_1:82659990-82660009 Msa0380720:CDS 30.0%
! AATCTGTTGATTATCACGTT+TGG - chr3_1:82660034-82660053 None:intergenic 30.0%
TATCTTGAGAATGTTCAAAG+AGG + chr3_1:82660236-82660255 Msa0380720:CDS 30.0%
ATAAGCTATTTACCCAAACA+AGG - chr3_1:82660509-82660528 None:intergenic 30.0%
! TTTTGTATAAGCTATCTTGG+AGG + chr3_1:82660618-82660637 Msa0380720:intron 30.0%
CATACGTGTTATCAGTAATT+TGG - chr3_1:82660981-82661000 None:intergenic 30.0%
!! GCTTCCATTTTTAATACCTA+CGG - chr3_1:82661121-82661140 None:intergenic 30.0%
! TAAGTCCCTAAACTTTGAAA+TGG + chr3_1:82661150-82661169 Msa0380720:intron 30.0%
!! AAGTCCCTAAACTTTGAAAT+GGG + chr3_1:82661151-82661170 Msa0380720:intron 30.0%
ATAACAGTTGCACATAAACA+TGG - chr3_1:82661270-82661289 None:intergenic 30.0%
ACAATTAGTAAACACAACAC+AGG - chr3_1:82661387-82661406 None:intergenic 30.0%
CAGATATTCAAACTGCAAAT+TGG - chr3_1:82661433-82661452 None:intergenic 30.0%
TTCTTGCCAGATTTATTATC+CGG + chr3_1:82661535-82661554 Msa0380720:CDS 30.0%
!!! ATTGACTTGAAATCGTTTTC+TGG + chr3_1:82661856-82661875 Msa0380720:intron 30.0%
TTCATTGCTGACCAATATAA+AGG + chr3_1:82661978-82661997 Msa0380720:exon 30.0%
CCACAAAAAGTCCTTTATAT+TGG - chr3_1:82661992-82662011 None:intergenic 30.0%
! CCAATATAAAGGACTTTTTG+TGG + chr3_1:82661989-82662008 Msa0380720:three_prime_UTR 30.0%
! ATATAAAGGACTTTTTGTGG+TGG + chr3_1:82661992-82662011 Msa0380720:three_prime_UTR 30.0%
! TATAAAGGACTTTTTGTGGT+GGG + chr3_1:82661993-82662012 Msa0380720:three_prime_UTR 30.0%
! GTGGTGATACTTTCAAATTT+TGG + chr3_1:82662020-82662039 Msa0380720:three_prime_UTR 30.0%
! GGTGATACTTTCAAATTTTG+GGG + chr3_1:82662022-82662041 Msa0380720:three_prime_UTR 30.0%
! GTGATACTTTCAAATTTTGG+GGG + chr3_1:82662023-82662042 Msa0380720:three_prime_UTR 30.0%
!! TTCAAATTTTGGGGGTTATT+TGG + chr3_1:82662031-82662050 Msa0380720:three_prime_UTR 30.0%
! TGAATTTGTTTATCATGGAC+AGG - chr3_1:82662234-82662253 None:intergenic 30.0%
!! TTTTTGGTCAATTGCTGAAT+CGG + chr3_1:82662257-82662276 Msa0380720:three_prime_UTR 30.0%
GTTATACTGCAATAGTTAGT+GGG + chr3_1:82662279-82662298 Msa0380720:three_prime_UTR 30.0%
! TGCATATTTGGTGATACAAT+GGG + chr3_1:82662452-82662471 Msa0380720:three_prime_UTR 30.0%
TTACAGACTCAACATGATTA+TGG - chr3_1:82662482-82662501 None:intergenic 30.0%
!! GATAAGTGTGTTTGTTATCA+TGG - chr3_1:82662519-82662538 None:intergenic 30.0%
ATTGGAATCAATGGAAGGAA+GGG - chr3_1:82659980-82659999 None:intergenic 35.0%
AATTGGAATCAATGGAAGGA+AGG - chr3_1:82659981-82660000 None:intergenic 35.0%
! ATGAACCGGCTTATTCAAAT+TGG - chr3_1:82659998-82660017 None:intergenic 35.0%
!!! GCATTGATTTTGGTATGAAC+CGG - chr3_1:82660012-82660031 None:intergenic 35.0%
!!! ATCACGTTTGGCATTGATTT+TGG - chr3_1:82660022-82660041 None:intergenic 35.0%
TCGGAGATATCAGATACATA+AGG - chr3_1:82660063-82660082 None:intergenic 35.0%
AAAAAGAGAAGACCAATGGT+TGG + chr3_1:82660212-82660231 Msa0380720:CDS 35.0%
TTCTCAAGATAACCAACCAT+TGG - chr3_1:82660227-82660246 None:intergenic 35.0%
GCATTACATCAAACATGAGA+GGG + chr3_1:82660258-82660277 Msa0380720:CDS 35.0%
! ATTGGTTGATTGGTTAGTTG+AGG + chr3_1:82660283-82660302 Msa0380720:CDS 35.0%
CATGTGAACAATGTCATAAG+TGG + chr3_1:82660666-82660685 Msa0380720:intron 35.0%
TCAAGAGCAATGACATGAAT+AGG - chr3_1:82661023-82661042 None:intergenic 35.0%
! TTTTTAATACCTACGGTAGC+AGG - chr3_1:82661114-82661133 None:intergenic 35.0%
GCTACCGTAGGTATTAAAAA+TGG + chr3_1:82661114-82661133 Msa0380720:intron 35.0%
GTTGCCCATTTCAAAGTTTA+GGG - chr3_1:82661158-82661177 None:intergenic 35.0%
CCAATTACTTCATTTCTGCA+AGG - chr3_1:82661242-82661261 None:intergenic 35.0%
CCTTGCAGAAATGAAGTAAT+TGG + chr3_1:82661239-82661258 Msa0380720:intron 35.0%
AACTGTTATGTTTCCCTTTC+AGG + chr3_1:82661281-82661300 Msa0380720:intron 35.0%
TGACAAATACAAGCAGAACA+AGG + chr3_1:82661774-82661793 Msa0380720:CDS 35.0%
! TCTGGAACAGTTAAAATGTG+TGG + chr3_1:82661874-82661893 Msa0380720:intron 35.0%
!! AAGTAATGATTCGGTATCTC+TGG - chr3_1:82661917-82661936 None:intergenic 35.0%
TTATCTGCTTGTACTGCTAA+TGG + chr3_1:82662155-82662174 Msa0380720:three_prime_UTR 35.0%
GGTTATACTGCAATAGTTAG+TGG + chr3_1:82662278-82662297 Msa0380720:three_prime_UTR 35.0%
GATGAGTAGTTCAACTCTAA+AGG - chr3_1:82662428-82662447 None:intergenic 35.0%
TACTCATCTCTGTGCATATT+TGG + chr3_1:82662440-82662459 Msa0380720:three_prime_UTR 35.0%
! GTGCATATTTGGTGATACAA+TGG + chr3_1:82662451-82662470 Msa0380720:three_prime_UTR 35.0%
!! AGTGTGTTTGTTATCATGGT+AGG - chr3_1:82662515-82662534 None:intergenic 35.0%
! CTTCACTACACTCTGAAAGA+TGG + chr3_1:82659771-82659790 Msa0380720:exon 40.0%
!! TGGTTGTTGATTGGTGTTGT+TGG - chr3_1:82659821-82659840 None:intergenic 40.0%
AACTTGTGGTGGTTGTTGAT+TGG - chr3_1:82659830-82659849 None:intergenic 40.0%
TCAACAACCACCACAAGTTA+TGG + chr3_1:82659831-82659850 Msa0380720:CDS 40.0%
!! CTTTTGGGAACCATAACTTG+TGG - chr3_1:82659844-82659863 None:intergenic 40.0%
TTATGGTTCCCAAAAGACAG+AGG + chr3_1:82659848-82659867 Msa0380720:CDS 40.0%
CTACATTGGAGTTTCTGAGT+TGG - chr3_1:82659925-82659944 None:intergenic 40.0%
CAACTCAGAAACTCCAATGT+AGG + chr3_1:82659923-82659942 Msa0380720:CDS 40.0%
! GGTTAGGGTTTTTCCTACAT+TGG - chr3_1:82659939-82659958 None:intergenic 40.0%
TGGCTGAATTCTTGAGGTTA+GGG - chr3_1:82659954-82659973 None:intergenic 40.0%
CTCCATTGTGCGAAGATAAT+CGG - chr3_1:82660082-82660101 None:intergenic 40.0%
GCAGAAAAAGAGAAGACCAA+TGG + chr3_1:82660208-82660227 Msa0380720:CDS 40.0%
GGCATTACATCAAACATGAG+AGG + chr3_1:82660257-82660276 Msa0380720:CDS 40.0%
ATCAAACATGAGAGGGACAT+TGG + chr3_1:82660265-82660284 Msa0380720:CDS 40.0%
TGCTGACAGGTTCAATAGAT+AGG - chr3_1:82660373-82660392 None:intergenic 40.0%
!! ACAAAGATCTAGGCCTTGTT+TGG + chr3_1:82660493-82660512 Msa0380720:intron 40.0%
!! CAAAGATCTAGGCCTTGTTT+GGG + chr3_1:82660494-82660513 Msa0380720:intron 40.0%
! CTCTAAGGGCATGTTTGTAT+TGG - chr3_1:82660753-82660772 None:intergenic 40.0%
TACTGATAACACGTATGACC+TGG + chr3_1:82660984-82661003 Msa0380720:intron 40.0%
ATAGGATGTTTACCTCTTCC+AGG - chr3_1:82661005-82661024 None:intergenic 40.0%
GGTTGCCCATTTCAAAGTTT+AGG - chr3_1:82661159-82661178 None:intergenic 40.0%
TGTTATGTTTCCCTTTCAGG+AGG + chr3_1:82661284-82661303 Msa0380720:intron 40.0%
TTCCCTTTCAGGAGGTTTAT+TGG + chr3_1:82661292-82661311 Msa0380720:intron 40.0%
TCCCTTTCAGGAGGTTTATT+GGG + chr3_1:82661293-82661312 Msa0380720:intron 40.0%
TCCCAATAAACCTCCTGAAA+GGG - chr3_1:82661297-82661316 None:intergenic 40.0%
ATCCCAATAAACCTCCTGAA+AGG - chr3_1:82661298-82661317 None:intergenic 40.0%
GATTGCTTGTGAGAATCGAA+AGG + chr3_1:82661315-82661334 Msa0380720:CDS 40.0%
!! TCTTGCTGACTTAAGCTTGT+TGG + chr3_1:82661461-82661480 Msa0380720:CDS 40.0%
TTGTATATGTCAAGAAGGGC+AGG + chr3_1:82661733-82661752 Msa0380720:CDS 40.0%
! TTTGTCACGAACAGCTTTGA+AGG - chr3_1:82661761-82661780 None:intergenic 40.0%
TGTTAATACAGCCTATGCTC+TGG + chr3_1:82662205-82662224 Msa0380720:three_prime_UTR 40.0%
GTTAATACAGCCTATGCTCT+GGG + chr3_1:82662206-82662225 Msa0380720:three_prime_UTR 40.0%
TTAATACAGCCTATGCTCTG+GGG + chr3_1:82662207-82662226 Msa0380720:three_prime_UTR 40.0%
!! GTTGTTGGCTCTCTTCTTTG+AGG - chr3_1:82659806-82659825 None:intergenic 45.0%
! TTGGGAACCATAACTTGTGG+TGG - chr3_1:82659841-82659860 None:intergenic 45.0%
GTGGCTGAATTCTTGAGGTT+AGG - chr3_1:82659955-82659974 None:intergenic 45.0%
ATCAATGGAAGGAAGGGTTG+TGG - chr3_1:82659974-82659993 None:intergenic 45.0%
CTCCGATTATCTTCGCACAA+TGG + chr3_1:82660077-82660096 Msa0380720:CDS 45.0%
CGATTATCTTCGCACAATGG+AGG + chr3_1:82660080-82660099 Msa0380720:CDS 45.0%
! TGAGAGGGACATTGGTTGAT+TGG + chr3_1:82660273-82660292 Msa0380720:CDS 45.0%
TGAAGCTTAGATCTGCTGAC+AGG - chr3_1:82660386-82660405 None:intergenic 45.0%
AGATCTAAGCTTCAGCTGCT+TGG + chr3_1:82660392-82660411 Msa0380720:CDS 45.0%
AAGCCTCATCACACTAAGTG+GGG - chr3_1:82660845-82660864 None:intergenic 45.0%
CAAGCCTCATCACACTAAGT+GGG - chr3_1:82660846-82660865 None:intergenic 45.0%
TCAAGCCTCATCACACTAAG+TGG - chr3_1:82660847-82660866 None:intergenic 45.0%
CTTAGTGTGATGAGGCTTGA+TGG + chr3_1:82660847-82660866 Msa0380720:intron 45.0%
TGAAGAAATCACCTCACCGA+AGG + chr3_1:82660942-82660961 Msa0380720:intron 45.0%
TAACACGTATGACCTGGAAG+AGG + chr3_1:82660990-82661009 Msa0380720:intron 45.0%
TCCAGGCCGGATAATAAATC+TGG - chr3_1:82661544-82661563 None:intergenic 45.0%
TTTACCCAAGGATGTACTCC+AGG - chr3_1:82661561-82661580 None:intergenic 45.0%
GTAACCCAAGCATTCACACA+GGG - chr3_1:82661671-82661690 None:intergenic 45.0%
TGTAACCCAAGCATTCACAC+AGG - chr3_1:82661672-82661691 None:intergenic 45.0%
TGAATGCTTGGGTTACACGT+CGG + chr3_1:82661675-82661694 Msa0380720:CDS 45.0%
!! AGGACTTTTTGTGGTGGGAT+AGG + chr3_1:82661998-82662017 Msa0380720:three_prime_UTR 45.0%
!! ACTTTTTGTGGTGGGATAGG+TGG + chr3_1:82662001-82662020 Msa0380720:three_prime_UTR 45.0%
AAGCAGTCAGCACCAGTAAT+GGG - chr3_1:82662120-82662139 None:intergenic 45.0%
!!! TTATTTTATTTACAATTTTT+AGG + chr3_1:82660152-82660171 Msa0380720:intron 5.0%
!!! TTATAATTATTTCTTATTAT+TGG + chr3_1:82661827-82661846 Msa0380720:intron 5.0%
GGGTTGTGGCTGAATTCTTG+AGG - chr3_1:82659960-82659979 None:intergenic 50.0%
! ACTGACAGATGAAGCGTCTC+TGG - chr3_1:82660332-82660351 None:intergenic 50.0%
ACTAAGTGGGGTCTGCTACA+TGG - chr3_1:82660833-82660852 None:intergenic 50.0%
AGACCCCACTTAGTGTGATG+AGG + chr3_1:82660839-82660858 Msa0380720:intron 50.0%
TGGCAGAAATCCACTGCCTT+CGG - chr3_1:82660961-82660980 None:intergenic 50.0%
TGTTGTCTTCCTGCTACCGT+AGG + chr3_1:82661102-82661121 Msa0380720:intron 50.0%
GCCAGATTTATTATCCGGCC+TGG + chr3_1:82661540-82661559 Msa0380720:CDS 50.0%
TTCGGTATCTCTGGCGATGA+AGG - chr3_1:82661908-82661927 None:intergenic 50.0%
AAATCCAGTCCAGCAGCATG+TGG - chr3_1:82662081-82662100 None:intergenic 50.0%
!! CATGCTGCTGGACTGGATTT+TGG + chr3_1:82662081-82662100 Msa0380720:three_prime_UTR 50.0%
CAAGCAGTCAGCACCAGTAA+TGG - chr3_1:82662121-82662140 None:intergenic 50.0%
AATCACCTCACCGAAGGCAG+TGG + chr3_1:82660948-82660967 Msa0380720:intron 55.0%
GAAATCCACTGCCTTCGGTG+AGG - chr3_1:82660956-82660975 None:intergenic 55.0%
GCCATCTGTTGTCGCTGCAT+CGG + chr3_1:82661506-82661525 Msa0380720:CDS 55.0%
ACCGATGCAGCGACAACAGA+TGG - chr3_1:82661510-82661529 None:intergenic 55.0%
TCCGTCCCTGTGTGAATGCT+TGG + chr3_1:82661663-82661682 Msa0380720:CDS 55.0%
CCCAAGCATTCACACAGGGA+CGG - chr3_1:82661667-82661686 None:intergenic 55.0%
CCGTCCCTGTGTGAATGCTT+GGG + chr3_1:82661664-82661683 Msa0380720:CDS 55.0%
GTATCTCTGGCGATGAAGGC+AGG - chr3_1:82661904-82661923 None:intergenic 55.0%
TACTGATGGCCACATGCTGC+TGG + chr3_1:82662069-82662088 Msa0380720:three_prime_UTR 55.0%
TCCGGCCTGGAGTACATCCT+TGG + chr3_1:82661553-82661572 Msa0380720:CDS 60.0%
CCCAAGGATGTACTCCAGGC+CGG - chr3_1:82661557-82661576 None:intergenic 60.0%
CCGGCCTGGAGTACATCCTT+GGG + chr3_1:82661554-82661573 Msa0380720:CDS 60.0%
CACAGGGACGGAGTCTGCAA+TGG - chr3_1:82661655-82661674 None:intergenic 60.0%
ATGGCCACATGCTGCTGGAC+TGG + chr3_1:82662074-82662093 Msa0380720:three_prime_UTR 60.0%
TGGACAGGACCCCAGAGCAT+AGG - chr3_1:82662219-82662238 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 82659732 82662564 82659732 ID=Msa0380720;Name=Msa0380720
chr3_1 mRNA 82659732 82662564 82659732 ID=Msa0380720-mRNA-1;Parent=Msa0380720;Name=Msa0380720-mRNA-1;_AED=0.30;_eAED=0.30;_QI=58|0.8|1|1|1|1|6|576|322
chr3_1 exon 82659732 82660101 82659732 ID=Msa0380720-mRNA-1:exon:21078;Parent=Msa0380720-mRNA-1
chr3_1 exon 82660206 82660438 82660206 ID=Msa0380720-mRNA-1:exon:21079;Parent=Msa0380720-mRNA-1
chr3_1 exon 82661303 82661336 82661303 ID=Msa0380720-mRNA-1:exon:21080;Parent=Msa0380720-mRNA-1
chr3_1 exon 82661426 82661575 82661426 ID=Msa0380720-mRNA-1:exon:21081;Parent=Msa0380720-mRNA-1
chr3_1 exon 82661661 82661795 82661661 ID=Msa0380720-mRNA-1:exon:21082;Parent=Msa0380720-mRNA-1
chr3_1 exon 82661884 82662564 82661884 ID=Msa0380720-mRNA-1:exon:21083;Parent=Msa0380720-mRNA-1
chr3_1 five_prime_UTR 82659732 82659789 82659732 ID=Msa0380720-mRNA-1:five_prime_utr;Parent=Msa0380720-mRNA-1
chr3_1 CDS 82659790 82660101 82659790 ID=Msa0380720-mRNA-1:cds;Parent=Msa0380720-mRNA-1
chr3_1 CDS 82660206 82660438 82660206 ID=Msa0380720-mRNA-1:cds;Parent=Msa0380720-mRNA-1
chr3_1 CDS 82661303 82661336 82661303 ID=Msa0380720-mRNA-1:cds;Parent=Msa0380720-mRNA-1
chr3_1 CDS 82661426 82661575 82661426 ID=Msa0380720-mRNA-1:cds;Parent=Msa0380720-mRNA-1
chr3_1 CDS 82661661 82661795 82661661 ID=Msa0380720-mRNA-1:cds;Parent=Msa0380720-mRNA-1
chr3_1 CDS 82661884 82661988 82661884 ID=Msa0380720-mRNA-1:cds;Parent=Msa0380720-mRNA-1
chr3_1 three_prime_UTR 82661989 82662564 82661989 ID=Msa0380720-mRNA-1:three_prime_utr;Parent=Msa0380720-mRNA-1
Gene Sequence

>Msa0380720

ATGGAGACTCGTGCCTCAAAGAAGAGAGCCAACAACACCAATCAACAACCACCACAAGTTATGGTTCCCAAAAGACAGAGGATTGTTTTAGGTGAATTTTCCGATCTCAATCTTCCTGTTTTTGATTATCAACCAACTCAGAAACTCCAATGTAGGAAAAACCCTAACCTCAAGAATTCAGCCACAACCCTTCCTTCCATTGATTCCAATTTGAATAAGCCGGTTCATACCAAAATCAATGCCAAACGTGATAATCAACAGATTATTCAGCCTTATGTATCTGATATCTCCGATTATCTTCGCACAATGGAGATGCAGAAAAAGAGAAGACCAATGGTTGGTTATCTTGAGAATGTTCAAAGAGGCATTACATCAAACATGAGAGGGACATTGGTTGATTGGTTAGTTGAGGTTGCTGATGAATACAAGCTTCTTCCAGAGACGCTTCATCTGTCAGTTTCATACATTGACAGATTCCTATCTATTGAACCTGTCAGCAGATCTAAGCTTCAGCTGCTTGGTGTTTCTTCAATGCTCATTGCATCGAGGTTTATTGGGATTGCTTGTGAGAATCGAAAGAATTCCAATTTGCAGTTTGAATATCTGTGCAGCTATCTTGCTGACTTAAGCTTGTTGGACTATGAGTGTATAAGATTCTTGCCATCTGTTGTCGCTGCATCGGTTATATTTCTTGCCAGATTTATTATCCGGCCTGGAGTACATCCTTGGACTCCGTCCCTGTGTGAATGCTTGGGTTACACGTCGGCTGATTTAGAAGAATGTGTTATCATTTTACATGATTTGTATATGTCAAGAAGGGCAGGATCCTTCAAAGCTGTTCGTGACAAATACAAGCAGAACAAGTTAAAATGTGTGGCAAACCTGCCTTCATCGCCAGAGATACCGAATCATTACTTTGAAGAAGAGTCATGTAGTACAATTAACTTTGTGGATGAAGTTCATTGCTGA
Protein sequence

>Msa0380720

METRASKKRANNTNQQPPQVMVPKRQRIVLGEFSDLNLPVFDYQPTQKLQCRKNPNLKNSATTLPSIDSNLNKPVHTKINAKRDNQQIIQPYVSDISDYLRTMEMQKKRRPMVGYLENVQRGITSNMRGTLVDWLVEVADEYKLLPETLHLSVSYIDRFLSIEPVSRSKLQLLGVSSMLIASRFIGIACENRKNSNLQFEYLCSYLADLSLLDYECIRFLPSVVAASVIFLARFIIRPGVHPWTPSLCECLGYTSADLEECVIILHDLYMSRRAGSFKAVRDKYKQNKLKCVANLPSSPEIPNHYFEEESCSTINFVDEVHC*