AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0383370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383370 MtrunA17_Chr3g0139171 96.512 172 5 1 1 172 1 171 4.26e-122 341
Msa0383370 MtrunA17_Chr1g0147571 69.663 178 40 5 5 172 39 212 1.70e-79 235
Msa0383370 MtrunA17_Chr4g0000251 61.314 137 44 2 5 136 4 136 2.72e-46 149
Msa0383370 MtrunA17_Chr4g0009931 71.287 101 22 3 49 142 1 101 2.28e-41 134
Msa0383370 MtrunA17_Chr2g0301531 43.609 133 71 2 10 138 12 144 5.08e-30 107
Msa0383370 MtrunA17_Chr1g0156341 34.395 157 64 3 6 154 4 129 4.80e-23 89.4
Msa0383370 MtrunA17_Chr4g0070071 38.400 125 71 2 14 134 11 133 7.89e-23 89.0
Msa0383370 MtrunA17_Chr4g0036141 36.296 135 49 2 8 141 11 109 1.49e-18 77.4
Msa0383370 MtrunA17_Chr4g0036531 47.500 80 41 1 57 135 12 91 3.73e-17 72.4
Msa0383370 MtrunA17_Chr5g0396091 44.048 84 46 1 53 135 12 95 5.02e-17 72.4
Msa0383370 MtrunA17_Chr4g0036841 54.430 79 34 2 52 129 18 95 9.96e-17 71.6
Msa0383370 MtrunA17_Chr7g0214201 33.083 133 84 2 15 147 15 142 1.01e-16 73.2
Msa0383370 MtrunA17_Chr2g0311341 31.967 122 74 2 16 131 1 119 1.44e-16 72.4
Msa0383370 MtrunA17_Chr3g0121721 55.224 67 29 1 70 135 25 91 1.45e-16 70.9
Msa0383370 MtrunA17_Chr2g0281291 53.731 67 30 1 70 135 26 92 2.29e-16 70.5
Msa0383370 MtrunA17_Chr8g0358971 53.731 67 30 1 70 135 25 91 2.92e-16 70.1
Msa0383370 MtrunA17_Chr3g0131991 31.928 166 86 7 10 168 4 149 4.61e-16 71.2
Msa0383370 MtrunA17_Chr8g0358871 48.485 66 34 0 68 133 33 98 5.60e-16 70.1
Msa0383370 MtrunA17_Chr4g0036161 41.667 84 46 1 50 133 19 99 6.11e-16 69.7
Msa0383370 MtrunA17_Chr4g0036271 55.738 61 26 1 70 129 23 83 6.11e-16 69.3
Msa0383370 MtrunA17_Chr4g0036331 55.738 61 26 1 70 129 23 83 6.31e-16 69.3
Msa0383370 MtrunA17_Chr3g0145371 37.383 107 58 2 34 133 4 108 7.39e-16 69.7
Msa0383370 MtrunA17_Chr3g0121701 52.239 67 31 1 70 135 25 91 8.59e-16 68.9
Msa0383370 MtrunA17_Chr3g0121731 52.239 67 31 1 70 135 25 91 9.35e-16 68.9
Msa0383370 MtrunA17_Chr4g0036661 54.839 62 27 1 69 129 19 80 2.73e-15 67.4
Msa0383370 MtrunA17_Chr8g0358981 50.746 67 32 1 70 135 25 91 2.84e-15 67.4
Msa0383370 MtrunA17_Chr4g0036201 44.304 79 43 1 52 129 8 86 4.48e-15 67.0
Msa0383370 MtrunA17_Chr4g0036691 48.052 77 39 1 54 129 4 80 4.86e-15 66.6
Msa0383370 MtrunA17_Chr8g0359001 60.377 53 20 1 70 121 25 77 5.81e-15 66.2
Msa0383370 MtrunA17_Chr3g0142421 39.326 89 49 1 50 133 17 105 6.74e-15 67.0
Msa0383370 MtrunA17_Chr8g0346181 44.118 68 36 1 69 134 26 93 7.87e-15 67.0
Msa0383370 MtrunA17_Chr4g0036821 49.254 67 33 1 70 135 26 92 8.56e-15 66.2
Msa0383370 MtrunA17_Chr4g0036811 47.368 76 38 2 52 126 9 83 9.35e-15 66.2
Msa0383370 MtrunA17_Chr5g0405301 40.230 87 49 1 50 133 17 103 1.02e-14 66.6
Msa0383370 MtrunA17_Chr3g0139731 57.377 61 25 1 70 129 64 124 1.07e-14 67.4
Msa0383370 MtrunA17_Chr4g0036461 43.373 83 46 1 54 135 9 91 1.15e-14 65.9
Msa0383370 MtrunA17_Chr4g0036221 53.731 67 29 2 70 135 26 91 1.29e-14 65.9
Msa0383370 MtrunA17_Chr4g0036421 45.455 77 41 1 54 129 4 80 1.31e-14 65.5
Msa0383370 MtrunA17_Chr4g0036861 55.738 61 26 1 70 129 57 117 1.31e-14 66.6
Msa0383370 MtrunA17_Chr4g0036611 45.455 77 41 1 54 129 9 85 1.45e-14 65.9
Msa0383370 MtrunA17_Chr3g0121801 56.897 58 24 1 70 126 25 82 1.63e-14 65.5
Msa0383370 MtrunA17_Chr4g0036621 54.098 61 27 1 70 129 19 79 1.76e-14 65.5
Msa0383370 MtrunA17_Chr3g0127561 35.417 96 60 1 41 134 4 99 1.82e-14 66.2
Msa0383370 MtrunA17_Chr4g0036181 45.570 79 42 1 52 129 65 143 1.88e-14 67.0
Msa0383370 MtrunA17_Chr1g0178451 42.029 69 40 0 66 134 45 113 1.88e-14 66.6
Msa0383370 MtrunA17_Chr4g0036431 43.750 80 44 1 57 135 12 91 1.90e-14 65.5
Msa0383370 MtrunA17_Chr4g0036571 42.857 91 45 2 46 129 30 120 3.15e-14 65.9
Msa0383370 MtrunA17_Chr4g0036651 42.500 80 45 1 57 135 12 91 3.44e-14 64.7
Msa0383370 MtrunA17_Chr4g0036631 50.746 67 32 1 70 135 20 86 3.45e-14 64.7
Msa0383370 MtrunA17_Chr3g0139711 44.578 83 45 1 54 135 10 92 3.85e-14 64.7
Msa0383370 MtrunA17_Chr4g0036401 42.500 80 45 1 57 135 12 91 4.17e-14 64.7
Msa0383370 MtrunA17_Chr7g0276101 47.059 68 34 1 69 134 22 89 4.51e-14 64.7
Msa0383370 MtrunA17_Chr4g0036241 43.373 83 46 1 54 135 10 92 5.07e-14 64.3
Msa0383370 MtrunA17_Chr4g0036671 54.098 61 27 1 70 129 21 81 5.61e-14 63.9
Msa0383370 MtrunA17_Chr4g0036831 47.887 71 36 1 52 121 8 78 5.78e-14 64.3
Msa0383370 MtrunA17_Chr8g0359011 56.897 58 24 1 70 126 3 60 5.94e-14 63.5
Msa0383370 MtrunA17_Chr4g0036491 44.156 77 42 1 54 129 9 85 6.41e-14 63.9
Msa0383370 MtrunA17_Chr4g0036441 44.156 77 42 1 54 129 4 80 6.99e-14 63.9
Msa0383370 MtrunA17_Chr4g0036581 42.500 80 45 1 57 135 12 91 7.26e-14 63.9
Msa0383370 MtrunA17_Chr4g0036751 53.333 60 27 1 68 126 18 77 7.64e-14 63.5
Msa0383370 MtrunA17_Chr3g0121811 55.172 58 25 1 70 126 26 83 8.19e-14 63.9
Msa0383370 MtrunA17_Chr4g0036521 45.349 86 43 3 51 134 15 98 8.72e-14 63.9
Msa0383370 MtrunA17_Chr4g0036601 51.613 62 29 1 70 130 21 82 9.46e-14 63.5
Msa0383370 MtrunA17_Chr8g0389141 32.558 129 66 2 3 131 4 111 1.04e-13 65.1
Msa0383370 MtrunA17_Chr1g0179511 46.970 66 35 0 70 135 19 84 1.10e-13 64.3
Msa0383370 MtrunA17_Chr4g0036471 52.459 61 28 1 70 129 21 81 1.13e-13 63.2
Msa0383370 MtrunA17_Chr3g0139701 54.098 61 27 1 70 129 83 143 1.48e-13 64.7
Msa0383370 MtrunA17_Chr4g0036681 47.761 67 34 1 70 135 25 91 1.61e-13 63.2
Msa0383370 MtrunA17_Chr7g0266571 45.161 62 34 0 73 134 53 114 1.70e-13 63.9
Msa0383370 MtrunA17_Chr4g0036501 52.459 61 28 1 70 129 21 81 1.95e-13 62.8
Msa0383370 MtrunA17_Chr8g0389151 45.714 70 38 0 68 137 38 107 2.37e-13 63.9
Msa0383370 MtrunA17_Chr4g0036391 51.613 62 29 1 69 129 19 80 3.00e-13 62.0
Msa0383370 MtrunA17_Chr4g0036211 46.053 76 40 1 52 126 146 221 3.17e-13 65.5
Msa0383370 MtrunA17_Chr4g0036211 45.714 70 37 1 52 120 8 77 1.62e-11 60.8
Msa0383370 MtrunA17_Chr3g0145401 46.988 83 42 2 48 129 24 105 3.34e-13 62.8
Msa0383370 MtrunA17_Chr8g0372061 33.929 112 69 2 25 133 39 148 3.42e-13 63.5
Msa0383370 MtrunA17_Chr4g0036791 54.098 61 27 1 70 129 27 87 4.13e-13 62.0
Msa0383370 MtrunA17_Chr7g0266581 43.548 62 35 0 73 134 53 114 4.77e-13 62.8
Msa0383370 MtrunA17_Chr4g0036541 50.820 61 29 1 70 129 20 80 4.88e-13 61.6
Msa0383370 MtrunA17_Chr4g0036641 52.459 61 28 1 70 129 21 81 5.64e-13 61.2
Msa0383370 MtrunA17_Chr4g0036761 44.872 78 32 2 70 146 23 90 6.16e-13 61.2
Msa0383370 MtrunA17_Chr4g0036411 52.459 61 28 1 70 129 21 81 6.34e-13 61.2
Msa0383370 MtrunA17_Chr4g0036551 51.724 58 27 1 70 126 20 77 6.81e-13 61.2
Msa0383370 MtrunA17_Chr4g0036191 54.098 61 25 2 70 129 26 84 1.03e-12 60.8
Msa0383370 MtrunA17_Chr3g0121751 61.364 44 17 0 70 113 83 126 1.58e-12 62.0
Msa0383370 MtrunA17_Chr4g0036801 50.746 67 32 1 70 135 26 92 1.78e-12 60.5
Msa0383370 MtrunA17_Chr4g0011821 42.105 76 40 2 66 137 75 150 1.90e-12 62.0
Msa0383370 MtrunA17_Chr4g0036561 52.459 61 28 1 70 129 20 80 2.16e-12 60.1
Msa0383370 MtrunA17_Chr5g0396071 42.500 80 45 1 57 135 8 87 2.76e-12 59.7
Msa0383370 MtrunA17_Chr1g0199531 52.459 61 29 0 70 130 50 110 3.58e-12 60.1
Msa0383370 MtrunA17_Chr6g0481791 39.759 83 47 2 53 134 19 99 3.97e-12 59.7
Msa0383370 MtrunA17_Chr4g0036231 44.928 69 37 1 54 121 18 86 5.27e-12 59.3
Msa0383370 MtrunA17_Chr5g0396081 53.846 52 23 1 71 121 21 72 6.55e-12 58.5
Msa0383370 MtrunA17_Chr4g0000241 38.824 85 45 2 53 130 40 124 7.90e-12 60.1
Msa0383370 MtrunA17_Chr4g0036711 47.059 68 35 1 68 134 13 80 8.20e-12 58.2
Msa0383370 MtrunA17_Chr3g0121761 46.269 67 35 1 70 135 25 91 1.16e-11 58.2
Msa0383370 MtrunA17_Chr1g0180731 41.975 81 42 2 67 145 23 100 1.26e-11 58.2
Msa0383370 MtrunA17_Chr8g0358991 51.724 58 27 1 70 126 25 82 1.28e-11 58.2
Msa0383370 MtrunA17_Chr7g0264571 42.105 76 42 1 58 131 23 98 2.47e-11 57.8
Msa0383370 MtrunA17_Chr3g0121741 50.000 58 28 1 70 126 25 82 2.77e-11 57.0
Msa0383370 MtrunA17_Chr4g0036341 50.000 60 29 1 68 126 19 78 2.90e-11 57.0
Msa0383370 MtrunA17_Chr3g0145411 54.237 59 25 2 70 126 34 92 4.77e-11 57.0
Msa0383370 MtrunA17_Chr3g0121781 50.000 58 28 1 70 126 25 82 7.15e-11 56.2
Msa0383370 MtrunA17_Chr7g0230411 34.021 97 59 2 45 138 52 146 7.74e-11 57.4
Msa0383370 MtrunA17_Chr4g0036891 50.847 59 27 2 70 126 27 85 9.17e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0383370 AT4G31320.1 52.083 192 62 5 5 172 4 189 4.78e-58 180
Msa0383370 AT2G24400.1 55.080 187 53 6 5 172 4 178 2.31e-56 175
Msa0383370 AT5G20810.1 44.697 132 66 2 10 134 12 143 5.33e-29 105
Msa0383370 AT5G20810.2 44.697 132 66 2 10 134 12 143 6.80e-29 105
Msa0383370 AT3G43120.1 38.571 140 77 2 10 141 12 150 1.92e-28 103
Msa0383370 AT1G19840.1 37.500 128 50 1 6 133 7 104 3.03e-20 82.4
Msa0383370 AT4G34750.2 36.719 128 51 2 6 133 3 100 1.71e-19 80.5
Msa0383370 AT4G34750.1 36.719 128 51 2 6 133 3 100 1.71e-19 80.5
Msa0383370 AT5G10990.1 36.296 135 56 1 6 140 7 111 2.55e-19 80.1
Msa0383370 AT3G12830.1 35.345 116 70 2 16 131 1 111 3.84e-19 79.0
Msa0383370 AT1G79130.1 35.593 118 65 2 16 131 1 109 4.47e-18 76.3
Msa0383370 AT1G75590.1 37.500 128 51 2 6 133 7 105 2.00e-17 75.1
Msa0383370 AT1G56150.1 33.621 116 60 1 16 131 1 99 2.69e-17 73.6
Msa0383370 AT4G34760.1 39.326 89 49 1 50 133 18 106 5.16e-17 72.8
Msa0383370 AT4G36110.1 41.237 97 56 1 37 132 6 102 7.02e-17 72.4
Msa0383370 AT3G20220.1 51.515 66 31 1 66 130 43 108 2.64e-16 71.2
Msa0383370 AT3G61900.1 52.239 67 29 1 70 133 30 96 3.37e-16 71.6
Msa0383370 AT2G21210.2 44.118 102 49 3 28 126 58 154 2.77e-15 69.7
Msa0383370 AT1G75580.1 34.884 129 59 3 5 133 4 107 3.40e-15 68.2
Msa0383370 AT2G21210.1 48.193 83 35 3 47 126 10 87 3.97e-15 67.8
Msa0383370 AT2G16580.1 37.778 90 50 1 50 133 18 107 4.84e-15 67.8
Msa0383370 AT5G66260.1 40.000 85 50 1 50 133 13 97 1.12e-14 66.6
Msa0383370 AT4G38840.1 57.627 59 23 2 70 126 33 91 1.76e-14 66.2
Msa0383370 AT2G18010.1 46.032 63 34 0 70 132 48 110 2.55e-14 65.9
Msa0383370 AT2G21220.1 40.230 87 49 1 50 133 17 103 2.95e-14 65.5
Msa0383370 AT2G46690.1 42.857 70 37 1 70 136 24 93 5.03e-14 65.5
Msa0383370 AT1G19830.1 36.538 104 54 2 39 130 5 108 6.43e-14 65.1
Msa0383370 AT4G38860.1 38.835 103 58 3 36 133 2 104 6.99e-14 64.7
Msa0383370 AT4G34770.1 46.667 90 41 4 52 135 15 103 7.12e-14 64.7
Msa0383370 AT3G60690.1 42.683 82 40 2 68 146 86 163 1.12e-13 65.9
Msa0383370 AT4G00880.1 47.887 71 35 1 66 134 24 94 1.27e-13 64.3
Msa0383370 AT1G16510.1 42.424 66 38 0 68 133 52 117 4.93e-13 63.5
Msa0383370 AT4G34800.1 50.769 65 28 2 70 130 21 85 1.91e-12 60.5
Msa0383370 AT4G12410.1 42.667 75 40 1 66 137 72 146 3.55e-12 61.6
Msa0383370 AT5G18080.1 46.875 64 32 2 65 126 19 82 5.21e-12 59.3
Msa0383370 AT4G38825.1 44.444 63 33 2 69 129 22 84 6.28e-12 59.3
Msa0383370 AT4G13790.1 44.118 68 35 2 69 133 24 91 7.20e-12 58.9
Msa0383370 AT4G22620.1 43.056 72 38 1 69 137 78 149 1.25e-11 60.1
Msa0383370 AT3G51200.1 46.154 65 34 1 70 133 39 103 1.35e-11 58.5
Msa0383370 AT5G53590.1 41.429 70 36 1 70 134 46 115 1.81e-11 59.3
Msa0383370 AT5G18010.1 44.737 76 36 3 53 126 11 82 2.27e-11 57.8
Msa0383370 AT5G18030.1 46.032 63 32 2 66 126 18 80 2.67e-11 57.4
Msa0383370 AT4G34780.1 51.562 64 27 2 70 129 28 91 3.52e-11 57.8
Msa0383370 AT4G34810.1 41.975 81 42 2 55 130 33 113 5.00e-11 57.4
Msa0383370 AT5G18020.1 46.032 63 32 2 66 126 20 82 7.41e-11 56.2
Msa0383370 AT3G03840.1 37.634 93 49 3 52 135 3 95 9.84e-11 56.2

Find 35 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAAGCTGAAGAAGAATT+TGG 0.168030 3_1:+86273525 Msa0383370:CDS
TGTTATACCAACCGAGTATT+TGG 0.205141 3_1:+86273476 Msa0383370:CDS
CTACTCTTCTCCATTTCTTA+AGG 0.303486 3_1:-86273275 None:intergenic
TCTACTCAGAAATGTAGATT+TGG 0.343922 3_1:+86273636 Msa0383370:CDS
GAGAAGCTGAAGAAGAATTT+GGG 0.377927 3_1:+86273526 Msa0383370:CDS
TTCAACAAATCCTTAAGAAA+TGG 0.389134 3_1:+86273265 Msa0383370:CDS
GAATTTGGGTTTGAACAAAC+TGG 0.403966 3_1:+86273540 Msa0383370:CDS
TGATCCTCCTCCTTCCCGTT+CGG 0.422045 3_1:-86273394 None:intergenic
TTGGTATAACAAACCTCTTA+AGG 0.430949 3_1:-86273464 None:intergenic
ATTTGGCATTGAAAAGATGA+TGG 0.461164 3_1:+86273653 Msa0383370:CDS
GTGTTGGTGTAGACCTTAAG+AGG 0.467583 3_1:+86273451 Msa0383370:CDS
ATAATAACCAAGTTGGTTTG+AGG 0.468390 3_1:-86273685 None:intergenic
AACACTTTCAATGTCCGAAC+GGG 0.473602 3_1:+86273380 Msa0383370:CDS
CTTGAAGATGGTTGAAGGAA+AGG 0.483489 3_1:+86273605 Msa0383370:CDS
TACTGTTCCTCAAACCAACT+TGG 0.509698 3_1:+86273678 Msa0383370:CDS
GGCTACCTAGCCGTTTGTGT+TGG 0.517754 3_1:+86273435 Msa0383370:CDS
GTTGATGATAATAACCAAGT+TGG 0.520248 3_1:-86273692 None:intergenic
CAAACGGCTAGGTAGCCTTT+CGG 0.521166 3_1:-86273429 None:intergenic
TATCTGCACATTGGACTATG+AGG 0.522020 3_1:-86273714 None:intergenic
AGTATCTTGAAGATGGTTGA+AGG 0.527345 3_1:+86273600 Msa0383370:CDS
AAGGTCTACACCAACACAAA+CGG 0.542797 3_1:-86273445 None:intergenic
GTTTGAGAGTATCTTGAAGA+TGG 0.543069 3_1:+86273593 Msa0383370:CDS
TTTGGCATTGAAAAGATGAT+GGG 0.560948 3_1:+86273654 Msa0383370:CDS
ATGTCCGAACGGGAAGGAGG+AGG 0.563260 3_1:+86273390 Msa0383370:CDS
TCAAACAACGCGGTTCCGAA+AGG 0.566250 3_1:+86273414 Msa0383370:CDS
TTCTCTGAGTAATATATGAA+AGG 0.569528 3_1:-86273508 None:intergenic
TGATGAGCCAAATACTCGGT+TGG 0.591052 3_1:-86273483 None:intergenic
GGCTTGATGAGCCAAATACT+CGG 0.601541 3_1:-86273487 None:intergenic
ATGACAAACTATCTGCACAT+TGG 0.610970 3_1:-86273723 None:intergenic
GAACACTTTCAATGTCCGAA+CGG 0.618386 3_1:+86273379 Msa0383370:CDS
TCAATGTCCGAACGGGAAGG+AGG 0.628171 3_1:+86273387 Msa0383370:CDS
CTACACCAACACAAACGGCT+AGG 0.632675 3_1:-86273440 None:intergenic
CTTTCAATGTCCGAACGGGA+AGG 0.652936 3_1:+86273384 Msa0383370:CDS
TCAAACACTGAAACTTCACA+AGG 0.665855 3_1:-86273576 None:intergenic
AGGAGGAGGATCAAACAACG+CGG 0.824181 3_1:+86273404 Msa0383370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATTTCTCTGATTTTGTT+AGG - chr3_1:86273240-86273259 None:intergenic 25.0%
! TTCAACAAATCCTTAAGAAA+TGG + chr3_1:86273265-86273284 Msa0383370:CDS 25.0%
!!! TCTGTTTGTTTTTGAAGTAT+TGG - chr3_1:86273301-86273320 None:intergenic 25.0%
!! AAACTTTATGCTTTTACTAG+TGG - chr3_1:86273352-86273371 None:intergenic 25.0%
! TTCTCTGAGTAATATATGAA+AGG - chr3_1:86273511-86273530 None:intergenic 25.0%
! CTGATTTTGTTAGGTTTCTT+TGG - chr3_1:86273231-86273250 None:intergenic 30.0%
TTGGTATAACAAACCTCTTA+AGG - chr3_1:86273467-86273486 None:intergenic 30.0%
TCTACTCAGAAATGTAGATT+TGG + chr3_1:86273636-86273655 Msa0383370:CDS 30.0%
! ATTTGGCATTGAAAAGATGA+TGG + chr3_1:86273653-86273672 Msa0383370:CDS 30.0%
! TTTGGCATTGAAAAGATGAT+GGG + chr3_1:86273654-86273673 Msa0383370:CDS 30.0%
!! ATAATAACCAAGTTGGTTTG+AGG - chr3_1:86273688-86273707 None:intergenic 30.0%
!! GTTGATGATAATAACCAAGT+TGG - chr3_1:86273695-86273714 None:intergenic 30.0%
CTACTCTTCTCCATTTCTTA+AGG - chr3_1:86273278-86273297 None:intergenic 35.0%
TGTTATACCAACCGAGTATT+TGG + chr3_1:86273476-86273495 Msa0383370:CDS 35.0%
AGAGAAGCTGAAGAAGAATT+TGG + chr3_1:86273525-86273544 Msa0383370:CDS 35.0%
GAGAAGCTGAAGAAGAATTT+GGG + chr3_1:86273526-86273545 Msa0383370:CDS 35.0%
! GAATTTGGGTTTGAACAAAC+TGG + chr3_1:86273540-86273559 Msa0383370:CDS 35.0%
TCAAACACTGAAACTTCACA+AGG - chr3_1:86273579-86273598 None:intergenic 35.0%
!! GTTTGAGAGTATCTTGAAGA+TGG + chr3_1:86273593-86273612 Msa0383370:CDS 35.0%
AGTATCTTGAAGATGGTTGA+AGG + chr3_1:86273600-86273619 Msa0383370:CDS 35.0%
GAACACTTTCAATGTCCGAA+CGG + chr3_1:86273379-86273398 Msa0383370:CDS 40.0%
AACACTTTCAATGTCCGAAC+GGG + chr3_1:86273380-86273399 Msa0383370:CDS 40.0%
AAGGTCTACACCAACACAAA+CGG - chr3_1:86273448-86273467 None:intergenic 40.0%
CTTGAAGATGGTTGAAGGAA+AGG + chr3_1:86273605-86273624 Msa0383370:CDS 40.0%
TACTGTTCCTCAAACCAACT+TGG + chr3_1:86273678-86273697 Msa0383370:CDS 40.0%
TATCTGCACATTGGACTATG+AGG - chr3_1:86273717-86273736 None:intergenic 40.0%
! GTGTTGGTGTAGACCTTAAG+AGG + chr3_1:86273451-86273470 Msa0383370:CDS 45.0%
TGATGAGCCAAATACTCGGT+TGG - chr3_1:86273486-86273505 None:intergenic 45.0%
GGCTTGATGAGCCAAATACT+CGG - chr3_1:86273490-86273509 None:intergenic 45.0%
CTTTCAATGTCCGAACGGGA+AGG + chr3_1:86273384-86273403 Msa0383370:CDS 50.0%
AGGAGGAGGATCAAACAACG+CGG + chr3_1:86273404-86273423 Msa0383370:CDS 50.0%
TCAAACAACGCGGTTCCGAA+AGG + chr3_1:86273414-86273433 Msa0383370:CDS 50.0%
CAAACGGCTAGGTAGCCTTT+CGG - chr3_1:86273432-86273451 None:intergenic 50.0%
CTACACCAACACAAACGGCT+AGG - chr3_1:86273443-86273462 None:intergenic 50.0%
TCAATGTCCGAACGGGAAGG+AGG + chr3_1:86273387-86273406 Msa0383370:CDS 55.0%
TGATCCTCCTCCTTCCCGTT+CGG - chr3_1:86273397-86273416 None:intergenic 55.0%
GGCTACCTAGCCGTTTGTGT+TGG + chr3_1:86273435-86273454 Msa0383370:CDS 55.0%
ATGTCCGAACGGGAAGGAGG+AGG + chr3_1:86273390-86273409 Msa0383370:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 86273219 86273737 86273219 ID=Msa0383370;Name=Msa0383370
chr3_1 mRNA 86273219 86273737 86273219 ID=Msa0383370-mRNA-1;Parent=Msa0383370;Name=Msa0383370-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|172
chr3_1 exon 86273219 86273737 86273219 ID=Msa0383370-mRNA-1:exon:22746;Parent=Msa0383370-mRNA-1
chr3_1 CDS 86273219 86273737 86273219 ID=Msa0383370-mRNA-1:cds;Parent=Msa0383370-mRNA-1
Gene Sequence

>Msa0383370

ATGGAAACACCAAAGAAACCTAACAAAATCAGAGAAATTGTTAGACTTCAACAAATCCTTAAGAAATGGAGAAGAGTAGCCAATACTTCAAAAACAAACAGAAGCAGCAGCAACAACAATAGCAACAGTACCACTAGTAAAAGCATAAAGTTTCTTAAAAGAACACTTTCAATGTCCGAACGGGAAGGAGGAGGATCAAACAACGCGGTTCCGAAAGGCTACCTAGCCGTTTGTGTTGGTGTAGACCTTAAGAGGTTTGTTATACCAACCGAGTATTTGGCTCATCAAGCCTTTCATATATTACTCAGAGAAGCTGAAGAAGAATTTGGGTTTGAACAAACTGGTGTTCTGAGAATTCCTTGTGAAGTTTCAGTGTTTGAGAGTATCTTGAAGATGGTTGAAGGAAAGGATAGATTTTCTACTCAGAAATGTAGATTTGGCATTGAAAAGATGATGGGATACTGTTCCTCAAACCAACTTGGTTATTATCATCAACCTCATAGTCCAATGTGCAGATAG
Protein sequence

>Msa0383370

METPKKPNKIREIVRLQQILKKWRRVANTSKTNRSSSNNNSNSTTSKSIKFLKRTLSMSEREGGGSNNAVPKGYLAVCVGVDLKRFVIPTEYLAHQAFHILLREAEEEFGFEQTGVLRIPCEVSVFESILKMVEGKDRFSTQKCRFGIEKMMGYCSSNQLGYYHQPHSPMCR*