AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0386720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0386720 MtrunA17_Chr1g0175161 84.685 222 5 1 4 196 121 342 3.68e-126 359
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0386720 AT3G57990.1 35.043 234 84 9 25 191 134 366 6.05e-27 105

Find 71 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCGGGAAGGTGCCGTCGGC+TGG 0.220747 3_1:-90488244 Msa0386720:CDS
TTTCTTGTGATGGATAAAAT+TGG 0.227367 3_1:-90488079 Msa0386720:CDS
TCCGCAAAACGGTGGCGTTT+TGG 0.257586 3_1:-90488323 Msa0386720:CDS
CTGAGGTTGCGGCGAGGACT+AGG 0.264777 3_1:-90488195 Msa0386720:CDS
TAGGGTTCCGGCTGAGGTTA+AGG 0.286798 3_1:-90488128 Msa0386720:CDS
GAGGACTAGGGTTCCGATTA+GGG 0.309618 3_1:-90488182 Msa0386720:CDS
CGAGGACTAGGGTTCCGATT+AGG 0.329022 3_1:-90488183 Msa0386720:CDS
GTTAAGGGCGAAAGCGCGTT+TGG 0.350617 3_1:-90488112 Msa0386720:CDS
TCGGGAAGGTGCCGTCGGCT+GGG 0.374474 3_1:-90488243 Msa0386720:CDS
GGCTGATGCGAGCAAGGAAT+TGG 0.376840 3_1:-90487766 Msa0386720:CDS
CGGGAAGGTGCCGTCGGCTG+GGG 0.380207 3_1:-90488242 Msa0386720:CDS
GGAGGAGGGAAAAGCTTTGA+GGG 0.400312 3_1:-90487849 Msa0386720:CDS
ACCGCGGCACGTCCCCTAAT+CGG 0.411961 3_1:+90488169 None:intergenic
TTCTTGTGATGGATAAAATT+GGG 0.414253 3_1:-90488078 Msa0386720:CDS
TTTGTTGAATCTCCGGTGAT+TGG 0.435435 3_1:-90488277 Msa0386720:CDS
TGGAGGAGGGAAAAGCTTTG+AGG 0.441086 3_1:-90487850 Msa0386720:CDS
CAACATGTCGAAATCAAATC+AGG 0.441154 3_1:-90488676 None:intergenic
AGAAAGAATGAGAAGAAAAC+CGG 0.442575 3_1:-90487825 Msa0386720:CDS
ATTTGAGTTTGTTGAATCTC+CGG 0.443909 3_1:-90488284 Msa0386720:CDS
AGGGTTCCGGCTGAGGTTAA+GGG 0.446628 3_1:-90488127 Msa0386720:CDS
GGAATGCTGTGGAGGATCTT+CGG 0.449412 3_1:-90487896 Msa0386720:CDS
TTAAGGGCGAAAGCGCGTTT+GGG 0.463653 3_1:-90488111 Msa0386720:CDS
CTTTCGCCCTTAACCTCAGC+CGG 0.466668 3_1:+90488121 None:intergenic
GGAGAGAATTCACTAGTGTA+AGG 0.470518 3_1:-90487875 Msa0386720:CDS
GAGGTTCTGCAGGCGGAGAA+TGG 0.475648 3_1:-90487927 Msa0386720:intron
GAGGGTTCCGTTTCTTGTGA+TGG 0.480532 3_1:-90488089 Msa0386720:CDS
GTGATTGGTGGGTTTAGTGT+CGG 0.483284 3_1:-90488262 Msa0386720:CDS
GATTTGAAGAATTTGAAGAA+AGG 0.489031 3_1:-90488028 Msa0386720:CDS
GGTTATGCGTTTCCGCAAAA+CGG 0.490258 3_1:-90488334 Msa0386720:CDS
TGATTGGTGGGTTTAGTGTC+GGG 0.491545 3_1:-90488261 Msa0386720:CDS
TTCAAATCCCCACACTCCGC+TGG 0.499435 3_1:+90488043 None:intergenic
AACATTTGCCAGCGGAGTGT+GGG 0.501336 3_1:-90488051 Msa0386720:CDS
GGAATTGGAGAAGGCGTTGA+GGG 0.502532 3_1:-90487751 Msa0386720:CDS
TTGAGGGGAGCCACCACTGT+TGG 0.513960 3_1:-90487735 Msa0386720:CDS
GTTGAATCTCCGGTGATTGG+TGG 0.516218 3_1:-90488274 Msa0386720:CDS
TGAGGTTGCGGCGAGGACTA+GGG 0.516942 3_1:-90488194 Msa0386720:CDS
GAACATTTGCCAGCGGAGTG+TGG 0.526006 3_1:-90488052 Msa0386720:CDS
TTCACTAGTGTAAGGATTGG+AGG 0.529400 3_1:-90487867 Msa0386720:CDS
GAATTCACTAGTGTAAGGAT+TGG 0.533076 3_1:-90487870 Msa0386720:CDS
GAAGAATTTGAAGAAAGGAA+AGG 0.533083 3_1:-90488023 Msa0386720:intron
TCCGATTAGGGGACGTGCCG+CGG 0.544241 3_1:-90488170 Msa0386720:CDS
AGGGCGAAAGCGCGTTTGGG+AGG 0.549372 3_1:-90488108 Msa0386720:CDS
GACTGAGGCTGATGCGAGCA+AGG 0.549803 3_1:-90487772 Msa0386720:CDS
TTGAATCTCCGGTGATTGGT+GGG 0.555286 3_1:-90488273 Msa0386720:CDS
GGGCGAAAGCGCGTTTGGGA+GGG 0.557564 3_1:-90488107 Msa0386720:CDS
ACTAGTGTAAGGATTGGAGG+AGG 0.570999 3_1:-90487864 Msa0386720:CDS
TAGTGTCGGGAAGGTGCCGT+CGG 0.576506 3_1:-90488248 Msa0386720:CDS
TCTGCAATCGCCCCAGCCGA+CGG 0.578829 3_1:+90488232 None:intergenic
ACACTAAACCCACCAATCAC+CGG 0.580138 3_1:+90488265 None:intergenic
TGGTGGGTTTAGTGTCGGGA+AGG 0.594609 3_1:-90488257 Msa0386720:CDS
TCAGCCTCAGTCGATTTCCC+AGG 0.596668 3_1:+90487783 None:intergenic
GAGGAGGGAAAAGCTTTGAG+GGG 0.601233 3_1:-90487848 Msa0386720:CDS
AATTCAAGCTCCAACAGTGG+TGG 0.602493 3_1:+90487725 None:intergenic
TTGAATTCAAGCTCCAACAG+TGG 0.608904 3_1:+90487722 None:intergenic
TGCGAGCAAGGAATTGGAGA+AGG 0.616295 3_1:-90487760 Msa0386720:CDS
ACATTTGCCAGCGGAGTGTG+GGG 0.619702 3_1:-90488050 Msa0386720:CDS
TCTTGTGATGGATAAAATTG+GGG 0.621136 3_1:-90488077 Msa0386720:CDS
AGGAATTGGAGAAGGCGTTG+AGG 0.624601 3_1:-90487752 Msa0386720:CDS
TATGCGTTTCCGCAAAACGG+TGG 0.624687 3_1:-90488331 Msa0386720:CDS
TGTGATGGATAAAATTGGGG+TGG 0.626777 3_1:-90488074 Msa0386720:CDS
GAAAGAATGAGAAGAAAACC+GGG 0.648044 3_1:-90487824 Msa0386720:CDS
GAATTGGAGAAGGCGTTGAG+GGG 0.650714 3_1:-90487750 Msa0386720:CDS
CGGGGACTGAGGTTGCGGCG+AGG 0.653848 3_1:-90488201 Msa0386720:CDS
TGGGGTGGAACATTTGCCAG+CGG 0.659065 3_1:-90488059 Msa0386720:CDS
AGGACTAGGGTTCCGATTAG+GGG 0.661644 3_1:-90488181 Msa0386720:CDS
AGGCGGAGAATGGATTGCTG+AGG 0.664151 3_1:-90487917 Msa0386720:CDS
ATTGCTGAGGAATGCTGTGG+AGG 0.665643 3_1:-90487904 Msa0386720:CDS
CTAGTGTAAGGATTGGAGGA+GGG 0.671920 3_1:-90487863 Msa0386720:CDS
TGGATTGCTGAGGAATGCTG+TGG 0.678621 3_1:-90487907 Msa0386720:CDS
ATAACCACTAAAATCTGACC+CGG 0.690957 3_1:+90487806 None:intergenic
TTATCCTGGGAAATCGACTG+AGG 0.704241 3_1:-90487787 Msa0386720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAAATTTGATTTCAATT+TGG - chr3_1:90487849-90487868 Msa0386720:CDS 15.0%
!!! AGAAAATTTGATTTCAATTT+GGG - chr3_1:90487850-90487869 Msa0386720:CDS 15.0%
!!! AAGAAAATTTGATTTCAATT+TGG - chr3_1:90487849-90487868 Msa0386720:CDS 15.0%
!!! AGAAAATTTGATTTCAATTT+GGG - chr3_1:90487850-90487869 Msa0386720:CDS 15.0%
!!! GTGTTTTTGTTAGTTTAAAA+TGG - chr3_1:90487789-90487808 Msa0386720:CDS 20.0%
!! TCTCAATTTGAAAATTGTAT+TGG - chr3_1:90487925-90487944 Msa0386720:intron 20.0%
!!! GTGTTTTTGTTAGTTTAAAA+TGG - chr3_1:90487789-90487808 Msa0386720:CDS 20.0%
!! TCTCAATTTGAAAATTGTAT+TGG - chr3_1:90487925-90487944 Msa0386720:intron 20.0%
!!! TGGTTTTGTTTAGATCAATA+AGG - chr3_1:90487809-90487828 Msa0386720:CDS 25.0%
! CACATTAATCAGTCAATTTA+GGG + chr3_1:90487893-90487912 None:intergenic 25.0%
!! ACAAAAACCATTTTAAACCT+AGG - chr3_1:90487992-90488011 Msa0386720:intron 25.0%
!! AGTTCGATTTTAGATGTTAA+GGG - chr3_1:90488045-90488064 Msa0386720:CDS 25.0%
!! ATTTTATCCATCACAAGAAA+CGG + chr3_1:90488321-90488340 None:intergenic 25.0%
!!! TTTCTTGTGATGGATAAAAT+TGG - chr3_1:90488321-90488340 Msa0386720:CDS 25.0%
!!! TTCTTGTGATGGATAAAATT+GGG - chr3_1:90488322-90488341 Msa0386720:CDS 25.0%
! GATTTGAAGAATTTGAAGAA+AGG - chr3_1:90488372-90488391 Msa0386720:intron 25.0%
!!! TGGTTTTGTTTAGATCAATA+AGG - chr3_1:90487809-90487828 Msa0386720:CDS 25.0%
! CACATTAATCAGTCAATTTA+GGG + chr3_1:90487893-90487912 None:intergenic 25.0%
!! ACAAAAACCATTTTAAACCT+AGG - chr3_1:90487992-90488011 Msa0386720:intron 25.0%
!! AGTTCGATTTTAGATGTTAA+GGG - chr3_1:90488045-90488064 Msa0386720:CDS 25.0%
!! ATTTTATCCATCACAAGAAA+CGG + chr3_1:90488321-90488340 None:intergenic 25.0%
!!! TTTCTTGTGATGGATAAAAT+TGG - chr3_1:90488321-90488340 Msa0386720:CDS 25.0%
!!! TTCTTGTGATGGATAAAATT+GGG - chr3_1:90488322-90488341 Msa0386720:CDS 25.0%
! GATTTGAAGAATTTGAAGAA+AGG - chr3_1:90488372-90488391 Msa0386720:intron 25.0%
AATAAGGTTTCTCTGTTAGT+TGG - chr3_1:90487825-90487844 Msa0386720:CDS 30.0%
GCACATTAATCAGTCAATTT+AGG + chr3_1:90487894-90487913 None:intergenic 30.0%
! AACCATTTTAAACCTAGGTT+TGG - chr3_1:90487997-90488016 Msa0386720:intron 30.0%
! ACCATTTTAAACCTAGGTTT+GGG - chr3_1:90487998-90488017 Msa0386720:intron 30.0%
! GAGTTCGATTTTAGATGTTA+AGG - chr3_1:90488044-90488063 Msa0386720:CDS 30.0%
!! ATTTGAGTTTGTTGAATCTC+CGG - chr3_1:90488116-90488135 Msa0386720:CDS 30.0%
!! TCTTGTGATGGATAAAATTG+GGG - chr3_1:90488323-90488342 Msa0386720:CDS 30.0%
GAAGAATTTGAAGAAAGGAA+AGG - chr3_1:90488377-90488396 Msa0386720:intron 30.0%
AATTTGAAGAAAGGAAAGGT+TGG - chr3_1:90488381-90488400 Msa0386720:intron 30.0%
AGAAAGAATGAGAAGAAAAC+CGG - chr3_1:90488575-90488594 Msa0386720:intron 30.0%
AATAAGGTTTCTCTGTTAGT+TGG - chr3_1:90487825-90487844 Msa0386720:CDS 30.0%
GCACATTAATCAGTCAATTT+AGG + chr3_1:90487894-90487913 None:intergenic 30.0%
! AACCATTTTAAACCTAGGTT+TGG - chr3_1:90487997-90488016 Msa0386720:intron 30.0%
! ACCATTTTAAACCTAGGTTT+GGG - chr3_1:90487998-90488017 Msa0386720:intron 30.0%
! GAGTTCGATTTTAGATGTTA+AGG - chr3_1:90488044-90488063 Msa0386720:CDS 30.0%
!! ATTTGAGTTTGTTGAATCTC+CGG - chr3_1:90488116-90488135 Msa0386720:CDS 30.0%
!! TCTTGTGATGGATAAAATTG+GGG - chr3_1:90488323-90488342 Msa0386720:CDS 30.0%
GAAGAATTTGAAGAAAGGAA+AGG - chr3_1:90488377-90488396 Msa0386720:intron 30.0%
AATTTGAAGAAAGGAAAGGT+TGG - chr3_1:90488381-90488400 Msa0386720:intron 30.0%
AGAAAGAATGAGAAGAAAAC+CGG - chr3_1:90488575-90488594 Msa0386720:intron 30.0%
! AATCAGTCAATTTAGGGCAT+CGG + chr3_1:90487887-90487906 None:intergenic 35.0%
! CCATTTTAAACCTAGGTTTG+GGG - chr3_1:90487999-90488018 Msa0386720:intron 35.0%
AAAGAAAAATCCCCAAACCT+AGG + chr3_1:90488012-90488031 None:intergenic 35.0%
GAATTCACTAGTGTAAGGAT+TGG - chr3_1:90488530-90488549 Msa0386720:intron 35.0%
GAAAGAATGAGAAGAAAACC+GGG - chr3_1:90488576-90488595 Msa0386720:intron 35.0%
ATAACCACTAAAATCTGACC+CGG + chr3_1:90488597-90488616 None:intergenic 35.0%
!! TCAGATTTTAGTGGTTATCC+TGG - chr3_1:90488599-90488618 Msa0386720:intron 35.0%
!! CAGATTTTAGTGGTTATCCT+GGG - chr3_1:90488600-90488619 Msa0386720:intron 35.0%
! AATCAGTCAATTTAGGGCAT+CGG + chr3_1:90487887-90487906 None:intergenic 35.0%
! CCATTTTAAACCTAGGTTTG+GGG - chr3_1:90487999-90488018 Msa0386720:intron 35.0%
AAAGAAAAATCCCCAAACCT+AGG + chr3_1:90488012-90488031 None:intergenic 35.0%
GAATTCACTAGTGTAAGGAT+TGG - chr3_1:90488530-90488549 Msa0386720:intron 35.0%
GAAAGAATGAGAAGAAAACC+GGG - chr3_1:90488576-90488595 Msa0386720:intron 35.0%
ATAACCACTAAAATCTGACC+CGG + chr3_1:90488597-90488616 None:intergenic 35.0%
!! TCAGATTTTAGTGGTTATCC+TGG - chr3_1:90488599-90488618 Msa0386720:intron 35.0%
!! CAGATTTTAGTGGTTATCCT+GGG - chr3_1:90488600-90488619 Msa0386720:intron 35.0%
CCCCAAACCTAGGTTTAAAA+TGG + chr3_1:90488002-90488021 None:intergenic 40.0%
TTTGTTGAATCTCCGGTGAT+TGG - chr3_1:90488123-90488142 Msa0386720:CDS 40.0%
!! TGTGATGGATAAAATTGGGG+TGG - chr3_1:90488326-90488345 Msa0386720:CDS 40.0%
! TTTTGCAGTGAAGAGGCAAA+TGG - chr3_1:90488451-90488470 Msa0386720:intron 40.0%
GGAGAGAATTCACTAGTGTA+AGG - chr3_1:90488525-90488544 Msa0386720:intron 40.0%
TTCACTAGTGTAAGGATTGG+AGG - chr3_1:90488533-90488552 Msa0386720:intron 40.0%
! AAAACCGGGTCAGATTTTAG+TGG - chr3_1:90488590-90488609 Msa0386720:intron 40.0%
CCCCAAACCTAGGTTTAAAA+TGG + chr3_1:90488002-90488021 None:intergenic 40.0%
TTTGTTGAATCTCCGGTGAT+TGG - chr3_1:90488123-90488142 Msa0386720:CDS 40.0%
!! TGTGATGGATAAAATTGGGG+TGG - chr3_1:90488326-90488345 Msa0386720:CDS 40.0%
! TTTTGCAGTGAAGAGGCAAA+TGG - chr3_1:90488451-90488470 Msa0386720:intron 40.0%
GGAGAGAATTCACTAGTGTA+AGG - chr3_1:90488525-90488544 Msa0386720:intron 40.0%
TTCACTAGTGTAAGGATTGG+AGG - chr3_1:90488533-90488552 Msa0386720:intron 40.0%
! AAAACCGGGTCAGATTTTAG+TGG - chr3_1:90488590-90488609 Msa0386720:intron 40.0%
GGTTATGCGTTTCCGCAAAA+CGG - chr3_1:90488066-90488085 Msa0386720:CDS 45.0%
TTGAATCTCCGGTGATTGGT+GGG - chr3_1:90488127-90488146 Msa0386720:CDS 45.0%
ACACTAAACCCACCAATCAC+CGG + chr3_1:90488138-90488157 None:intergenic 45.0%
!! GTGATTGGTGGGTTTAGTGT+CGG - chr3_1:90488138-90488157 Msa0386720:CDS 45.0%
!! TGATTGGTGGGTTTAGTGTC+GGG - chr3_1:90488139-90488158 Msa0386720:CDS 45.0%
!! GGCGATTGCAGAGTTGTTTT+CGG - chr3_1:90488179-90488198 Msa0386720:CDS 45.0%
!! GCGATTGCAGAGTTGTTTTC+GGG - chr3_1:90488180-90488199 Msa0386720:CDS 45.0%
!! CGATTGCAGAGTTGTTTTCG+GGG - chr3_1:90488181-90488200 Msa0386720:CDS 45.0%
!! TGAATTTTAGGTGGGGGGTT+AGG - chr3_1:90488253-90488272 Msa0386720:CDS 45.0%
!! GAATTTTAGGTGGGGGGTTA+GGG - chr3_1:90488254-90488273 Msa0386720:CDS 45.0%
CTTCACTGCAAAACTCGTCT+CGG + chr3_1:90488444-90488463 None:intergenic 45.0%
! AGACGAGTTTTGCAGTGAAG+AGG - chr3_1:90488444-90488463 Msa0386720:intron 45.0%
ACTAGTGTAAGGATTGGAGG+AGG - chr3_1:90488536-90488555 Msa0386720:intron 45.0%
CTAGTGTAAGGATTGGAGGA+GGG - chr3_1:90488537-90488556 Msa0386720:intron 45.0%
TTATCCTGGGAAATCGACTG+AGG - chr3_1:90488613-90488632 Msa0386720:intron 45.0%
GGTTATGCGTTTCCGCAAAA+CGG - chr3_1:90488066-90488085 Msa0386720:CDS 45.0%
TTGAATCTCCGGTGATTGGT+GGG - chr3_1:90488127-90488146 Msa0386720:CDS 45.0%
ACACTAAACCCACCAATCAC+CGG + chr3_1:90488138-90488157 None:intergenic 45.0%
!! GTGATTGGTGGGTTTAGTGT+CGG - chr3_1:90488138-90488157 Msa0386720:CDS 45.0%
!! TGATTGGTGGGTTTAGTGTC+GGG - chr3_1:90488139-90488158 Msa0386720:CDS 45.0%
!! GGCGATTGCAGAGTTGTTTT+CGG - chr3_1:90488179-90488198 Msa0386720:CDS 45.0%
!! GCGATTGCAGAGTTGTTTTC+GGG - chr3_1:90488180-90488199 Msa0386720:CDS 45.0%
!! CGATTGCAGAGTTGTTTTCG+GGG - chr3_1:90488181-90488200 Msa0386720:CDS 45.0%
!! TGAATTTTAGGTGGGGGGTT+AGG - chr3_1:90488253-90488272 Msa0386720:CDS 45.0%
!! GAATTTTAGGTGGGGGGTTA+GGG - chr3_1:90488254-90488273 Msa0386720:CDS 45.0%
CTTCACTGCAAAACTCGTCT+CGG + chr3_1:90488444-90488463 None:intergenic 45.0%
! AGACGAGTTTTGCAGTGAAG+AGG - chr3_1:90488444-90488463 Msa0386720:intron 45.0%
ACTAGTGTAAGGATTGGAGG+AGG - chr3_1:90488536-90488555 Msa0386720:intron 45.0%
CTAGTGTAAGGATTGGAGGA+GGG - chr3_1:90488537-90488556 Msa0386720:intron 45.0%
TTATCCTGGGAAATCGACTG+AGG - chr3_1:90488613-90488632 Msa0386720:intron 45.0%
!!! TTTGTATAATTTTTAATTTT+TGG + chr3_1:90487977-90487996 None:intergenic 5.0%
!!! TTTGTATAATTTTTAATTTT+TGG + chr3_1:90487977-90487996 None:intergenic 5.0%
TATGCGTTTCCGCAAAACGG+TGG - chr3_1:90488069-90488088 Msa0386720:CDS 50.0%
GTTGAATCTCCGGTGATTGG+TGG - chr3_1:90488126-90488145 Msa0386720:CDS 50.0%
!! AGAGTTGTTTTCGGGGACTG+AGG - chr3_1:90488188-90488207 Msa0386720:CDS 50.0%
GAGGACTAGGGTTCCGATTA+GGG - chr3_1:90488218-90488237 Msa0386720:CDS 50.0%
AGGACTAGGGTTCCGATTAG+GGG - chr3_1:90488219-90488238 Msa0386720:CDS 50.0%
!! TGCCGCGGTGAATTTTAGGT+GGG - chr3_1:90488245-90488264 Msa0386720:CDS 50.0%
! TTAAGGGCGAAAGCGCGTTT+GGG - chr3_1:90488289-90488308 Msa0386720:CDS 50.0%
GAGGGTTCCGTTTCTTGTGA+TGG - chr3_1:90488311-90488330 Msa0386720:CDS 50.0%
AACATTTGCCAGCGGAGTGT+GGG - chr3_1:90488349-90488368 Msa0386720:CDS 50.0%
!! AAAGGAAAGGTTGGTGCTGC+TGG - chr3_1:90488390-90488409 Msa0386720:intron 50.0%
TGCAGTGAAGAGGCAAATGG+AGG - chr3_1:90488454-90488473 Msa0386720:intron 50.0%
TGGATTGCTGAGGAATGCTG+TGG - chr3_1:90488493-90488512 Msa0386720:intron 50.0%
ATTGCTGAGGAATGCTGTGG+AGG - chr3_1:90488496-90488515 Msa0386720:intron 50.0%
GGAATGCTGTGGAGGATCTT+CGG - chr3_1:90488504-90488523 Msa0386720:intron 50.0%
TGGAGGAGGGAAAAGCTTTG+AGG - chr3_1:90488550-90488569 Msa0386720:intron 50.0%
! GGAGGAGGGAAAAGCTTTGA+GGG - chr3_1:90488551-90488570 Msa0386720:intron 50.0%
!! GAGGAGGGAAAAGCTTTGAG+GGG - chr3_1:90488552-90488571 Msa0386720:intron 50.0%
TGCGAGCAAGGAATTGGAGA+AGG - chr3_1:90488640-90488659 Msa0386720:intron 50.0%
!! AGGAATTGGAGAAGGCGTTG+AGG - chr3_1:90488648-90488667 Msa0386720:intron 50.0%
!! GGAATTGGAGAAGGCGTTGA+GGG - chr3_1:90488649-90488668 Msa0386720:intron 50.0%
!! GAATTGGAGAAGGCGTTGAG+GGG - chr3_1:90488650-90488669 Msa0386720:intron 50.0%
TATGCGTTTCCGCAAAACGG+TGG - chr3_1:90488069-90488088 Msa0386720:CDS 50.0%
GTTGAATCTCCGGTGATTGG+TGG - chr3_1:90488126-90488145 Msa0386720:CDS 50.0%
!! AGAGTTGTTTTCGGGGACTG+AGG - chr3_1:90488188-90488207 Msa0386720:CDS 50.0%
GAGGACTAGGGTTCCGATTA+GGG - chr3_1:90488218-90488237 Msa0386720:CDS 50.0%
AGGACTAGGGTTCCGATTAG+GGG - chr3_1:90488219-90488238 Msa0386720:CDS 50.0%
!! TGCCGCGGTGAATTTTAGGT+GGG - chr3_1:90488245-90488264 Msa0386720:CDS 50.0%
! TTAAGGGCGAAAGCGCGTTT+GGG - chr3_1:90488289-90488308 Msa0386720:CDS 50.0%
GAGGGTTCCGTTTCTTGTGA+TGG - chr3_1:90488311-90488330 Msa0386720:CDS 50.0%
AACATTTGCCAGCGGAGTGT+GGG - chr3_1:90488349-90488368 Msa0386720:CDS 50.0%
!! AAAGGAAAGGTTGGTGCTGC+TGG - chr3_1:90488390-90488409 Msa0386720:intron 50.0%
TGCAGTGAAGAGGCAAATGG+AGG - chr3_1:90488454-90488473 Msa0386720:intron 50.0%
TGGATTGCTGAGGAATGCTG+TGG - chr3_1:90488493-90488512 Msa0386720:intron 50.0%
ATTGCTGAGGAATGCTGTGG+AGG - chr3_1:90488496-90488515 Msa0386720:intron 50.0%
GGAATGCTGTGGAGGATCTT+CGG - chr3_1:90488504-90488523 Msa0386720:intron 50.0%
TGGAGGAGGGAAAAGCTTTG+AGG - chr3_1:90488550-90488569 Msa0386720:intron 50.0%
! GGAGGAGGGAAAAGCTTTGA+GGG - chr3_1:90488551-90488570 Msa0386720:intron 50.0%
!! GAGGAGGGAAAAGCTTTGAG+GGG - chr3_1:90488552-90488571 Msa0386720:intron 50.0%
TGCGAGCAAGGAATTGGAGA+AGG - chr3_1:90488640-90488659 Msa0386720:intron 50.0%
!! AGGAATTGGAGAAGGCGTTG+AGG - chr3_1:90488648-90488667 Msa0386720:intron 50.0%
!! GGAATTGGAGAAGGCGTTGA+GGG - chr3_1:90488649-90488668 Msa0386720:intron 50.0%
!! GAATTGGAGAAGGCGTTGAG+GGG - chr3_1:90488650-90488669 Msa0386720:intron 50.0%
! TCCGCAAAACGGTGGCGTTT+TGG - chr3_1:90488077-90488096 Msa0386720:CDS 55.0%
! CCCAAAACGCCACCGTTTTG+CGG + chr3_1:90488081-90488100 None:intergenic 55.0%
! CCGCAAAACGGTGGCGTTTT+GGG - chr3_1:90488078-90488097 Msa0386720:CDS 55.0%
! CGCAAAACGGTGGCGTTTTG+GGG - chr3_1:90488079-90488098 Msa0386720:CDS 55.0%
!! TGGTGGGTTTAGTGTCGGGA+AGG - chr3_1:90488143-90488162 Msa0386720:CDS 55.0%
!! GTTTTCGGGGACTGAGGTTG+CGG - chr3_1:90488194-90488213 Msa0386720:CDS 55.0%
CGAGGACTAGGGTTCCGATT+AGG - chr3_1:90488217-90488236 Msa0386720:CDS 55.0%
! GACGTGCCGCGGTGAATTTT+AGG - chr3_1:90488241-90488260 Msa0386720:CDS 55.0%
! GTGCCGCGGTGAATTTTAGG+TGG - chr3_1:90488244-90488263 Msa0386720:CDS 55.0%
!! GCCGCGGTGAATTTTAGGTG+GGG - chr3_1:90488246-90488265 Msa0386720:CDS 55.0%
CCCCCACCTAAAATTCACCG+CGG + chr3_1:90488250-90488269 None:intergenic 55.0%
!! CCGCGGTGAATTTTAGGTGG+GGG - chr3_1:90488247-90488266 Msa0386720:CDS 55.0%
!! CGCGGTGAATTTTAGGTGGG+GGG - chr3_1:90488248-90488267 Msa0386720:CDS 55.0%
TAGGGTTCCGGCTGAGGTTA+AGG - chr3_1:90488272-90488291 Msa0386720:CDS 55.0%
AGGGTTCCGGCTGAGGTTAA+GGG - chr3_1:90488273-90488292 Msa0386720:CDS 55.0%
CTTTCGCCCTTAACCTCAGC+CGG + chr3_1:90488282-90488301 None:intergenic 55.0%
! GTTAAGGGCGAAAGCGCGTT+TGG - chr3_1:90488288-90488307 Msa0386720:CDS 55.0%
TGGGGTGGAACATTTGCCAG+CGG - chr3_1:90488341-90488360 Msa0386720:CDS 55.0%
GAACATTTGCCAGCGGAGTG+TGG - chr3_1:90488348-90488367 Msa0386720:CDS 55.0%
ACATTTGCCAGCGGAGTGTG+GGG - chr3_1:90488350-90488369 Msa0386720:CDS 55.0%
TTCAAATCCCCACACTCCGC+TGG + chr3_1:90488360-90488379 None:intergenic 55.0%
!! AAGGTTGGTGCTGCTGGAGT+TGG - chr3_1:90488396-90488415 Msa0386720:intron 55.0%
GAGGCAAATGGAGGTTCTGC+AGG - chr3_1:90488463-90488482 Msa0386720:intron 55.0%
GCAAATGGAGGTTCTGCAGG+CGG - chr3_1:90488466-90488485 Msa0386720:intron 55.0%
!! AGGCGGAGAATGGATTGCTG+AGG - chr3_1:90488483-90488502 Msa0386720:intron 55.0%
! TCAGCCTCAGTCGATTTCCC+AGG + chr3_1:90488620-90488639 None:intergenic 55.0%
GGCTGATGCGAGCAAGGAAT+TGG - chr3_1:90488634-90488653 Msa0386720:intron 55.0%
! TCCGCAAAACGGTGGCGTTT+TGG - chr3_1:90488077-90488096 Msa0386720:CDS 55.0%
! CCCAAAACGCCACCGTTTTG+CGG + chr3_1:90488081-90488100 None:intergenic 55.0%
! CCGCAAAACGGTGGCGTTTT+GGG - chr3_1:90488078-90488097 Msa0386720:CDS 55.0%
! CGCAAAACGGTGGCGTTTTG+GGG - chr3_1:90488079-90488098 Msa0386720:CDS 55.0%
!! TGGTGGGTTTAGTGTCGGGA+AGG - chr3_1:90488143-90488162 Msa0386720:CDS 55.0%
!! GTTTTCGGGGACTGAGGTTG+CGG - chr3_1:90488194-90488213 Msa0386720:CDS 55.0%
CGAGGACTAGGGTTCCGATT+AGG - chr3_1:90488217-90488236 Msa0386720:CDS 55.0%
! GACGTGCCGCGGTGAATTTT+AGG - chr3_1:90488241-90488260 Msa0386720:CDS 55.0%
! GTGCCGCGGTGAATTTTAGG+TGG - chr3_1:90488244-90488263 Msa0386720:CDS 55.0%
!! GCCGCGGTGAATTTTAGGTG+GGG - chr3_1:90488246-90488265 Msa0386720:CDS 55.0%
CCCCCACCTAAAATTCACCG+CGG + chr3_1:90488250-90488269 None:intergenic 55.0%
!! CCGCGGTGAATTTTAGGTGG+GGG - chr3_1:90488247-90488266 Msa0386720:CDS 55.0%
!! CGCGGTGAATTTTAGGTGGG+GGG - chr3_1:90488248-90488267 Msa0386720:CDS 55.0%
TAGGGTTCCGGCTGAGGTTA+AGG - chr3_1:90488272-90488291 Msa0386720:CDS 55.0%
AGGGTTCCGGCTGAGGTTAA+GGG - chr3_1:90488273-90488292 Msa0386720:CDS 55.0%
CTTTCGCCCTTAACCTCAGC+CGG + chr3_1:90488282-90488301 None:intergenic 55.0%
! GTTAAGGGCGAAAGCGCGTT+TGG - chr3_1:90488288-90488307 Msa0386720:CDS 55.0%
TGGGGTGGAACATTTGCCAG+CGG - chr3_1:90488341-90488360 Msa0386720:CDS 55.0%
GAACATTTGCCAGCGGAGTG+TGG - chr3_1:90488348-90488367 Msa0386720:CDS 55.0%
ACATTTGCCAGCGGAGTGTG+GGG - chr3_1:90488350-90488369 Msa0386720:CDS 55.0%
TTCAAATCCCCACACTCCGC+TGG + chr3_1:90488360-90488379 None:intergenic 55.0%
!! AAGGTTGGTGCTGCTGGAGT+TGG - chr3_1:90488396-90488415 Msa0386720:intron 55.0%
GAGGCAAATGGAGGTTCTGC+AGG - chr3_1:90488463-90488482 Msa0386720:intron 55.0%
GCAAATGGAGGTTCTGCAGG+CGG - chr3_1:90488466-90488485 Msa0386720:intron 55.0%
!! AGGCGGAGAATGGATTGCTG+AGG - chr3_1:90488483-90488502 Msa0386720:intron 55.0%
! TCAGCCTCAGTCGATTTCCC+AGG + chr3_1:90488620-90488639 None:intergenic 55.0%
GGCTGATGCGAGCAAGGAAT+TGG - chr3_1:90488634-90488653 Msa0386720:intron 55.0%
!! TAGTGTCGGGAAGGTGCCGT+CGG - chr3_1:90488152-90488171 Msa0386720:CDS 60.0%
TGAGGTTGCGGCGAGGACTA+GGG - chr3_1:90488206-90488225 Msa0386720:CDS 60.0%
TAGGTGGGGGGTTAGGGTTC+CGG - chr3_1:90488260-90488279 Msa0386720:CDS 60.0%
GAGGTTCTGCAGGCGGAGAA+TGG - chr3_1:90488473-90488492 Msa0386720:intron 60.0%
GACTGAGGCTGATGCGAGCA+AGG - chr3_1:90488628-90488647 Msa0386720:intron 60.0%
TTGAGGGGAGCCACCACTGT+TGG - chr3_1:90488665-90488684 Msa0386720:intron 60.0%
!! TAGTGTCGGGAAGGTGCCGT+CGG - chr3_1:90488152-90488171 Msa0386720:CDS 60.0%
TGAGGTTGCGGCGAGGACTA+GGG - chr3_1:90488206-90488225 Msa0386720:CDS 60.0%
TAGGTGGGGGGTTAGGGTTC+CGG - chr3_1:90488260-90488279 Msa0386720:CDS 60.0%
GAGGTTCTGCAGGCGGAGAA+TGG - chr3_1:90488473-90488492 Msa0386720:intron 60.0%
GACTGAGGCTGATGCGAGCA+AGG - chr3_1:90488628-90488647 Msa0386720:intron 60.0%
TTGAGGGGAGCCACCACTGT+TGG - chr3_1:90488665-90488684 Msa0386720:intron 60.0%
TCTGCAATCGCCCCAGCCGA+CGG + chr3_1:90488171-90488190 None:intergenic 65.0%
CTGAGGTTGCGGCGAGGACT+AGG - chr3_1:90488205-90488224 Msa0386720:CDS 65.0%
TCCGATTAGGGGACGTGCCG+CGG - chr3_1:90488230-90488249 Msa0386720:CDS 65.0%
ACCGCGGCACGTCCCCTAAT+CGG + chr3_1:90488234-90488253 None:intergenic 65.0%
! AGGGCGAAAGCGCGTTTGGG+AGG - chr3_1:90488292-90488311 Msa0386720:CDS 65.0%
! GGGCGAAAGCGCGTTTGGGA+GGG - chr3_1:90488293-90488312 Msa0386720:CDS 65.0%
TCTGCAATCGCCCCAGCCGA+CGG + chr3_1:90488171-90488190 None:intergenic 65.0%
CTGAGGTTGCGGCGAGGACT+AGG - chr3_1:90488205-90488224 Msa0386720:CDS 65.0%
TCCGATTAGGGGACGTGCCG+CGG - chr3_1:90488230-90488249 Msa0386720:CDS 65.0%
ACCGCGGCACGTCCCCTAAT+CGG + chr3_1:90488234-90488253 None:intergenic 65.0%
! AGGGCGAAAGCGCGTTTGGG+AGG - chr3_1:90488292-90488311 Msa0386720:CDS 65.0%
! GGGCGAAAGCGCGTTTGGGA+GGG - chr3_1:90488293-90488312 Msa0386720:CDS 65.0%
!! TCGGGAAGGTGCCGTCGGCT+GGG - chr3_1:90488157-90488176 Msa0386720:CDS 70.0%
GGGGGTTAGGGTTCCGGCTG+AGG - chr3_1:90488266-90488285 Msa0386720:CDS 70.0%
!! TCGGGAAGGTGCCGTCGGCT+GGG - chr3_1:90488157-90488176 Msa0386720:CDS 70.0%
GGGGGTTAGGGTTCCGGCTG+AGG - chr3_1:90488266-90488285 Msa0386720:CDS 70.0%
!! GTCGGGAAGGTGCCGTCGGC+TGG - chr3_1:90488156-90488175 Msa0386720:CDS 75.0%
!! CGGGAAGGTGCCGTCGGCTG+GGG - chr3_1:90488158-90488177 Msa0386720:CDS 75.0%
CGGGGACTGAGGTTGCGGCG+AGG - chr3_1:90488199-90488218 Msa0386720:CDS 75.0%
!! GTCGGGAAGGTGCCGTCGGC+TGG - chr3_1:90488156-90488175 Msa0386720:CDS 75.0%
!! CGGGAAGGTGCCGTCGGCTG+GGG - chr3_1:90488158-90488177 Msa0386720:CDS 75.0%
CGGGGACTGAGGTTGCGGCG+AGG - chr3_1:90488199-90488218 Msa0386720:CDS 75.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 90487728 90488694 90487728 ID=Msa0386720;Name=Msa0386720
chr3_1 mRNA 90487728 90488694 90487728 ID=Msa0386720-mRNA-1;Parent=Msa0386720;Name=Msa0386720-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|0.66|1|1|3|0|196
chr3_1 exon 90488677 90488694 90488677 ID=Msa0386720-mRNA-1:exon:24999;Parent=Msa0386720-mRNA-1
chr3_1 exon 90488024 90488386 90488024 ID=Msa0386720-mRNA-1:exon:24998;Parent=Msa0386720-mRNA-1
chr3_1 exon 90487728 90487937 90487728 ID=Msa0386720-mRNA-1:exon:24997;Parent=Msa0386720-mRNA-1
chr3_1 CDS 90488677 90488694 90488677 ID=Msa0386720-mRNA-1:cds;Parent=Msa0386720-mRNA-1
chr3_1 CDS 90488024 90488386 90488024 ID=Msa0386720-mRNA-1:cds;Parent=Msa0386720-mRNA-1
chr3_1 CDS 90487728 90487937 90487728 ID=Msa0386720-mRNA-1:cds;Parent=Msa0386720-mRNA-1
Gene Sequence

>Msa0386720

ATGTCGAAATCAAATCAGAAAACTCAGAGTTCGATTTTAGATGTTAAGGGTTATGCGTTTCCGCAAAACGGTGGCGTTTTGGGGAATGATGATACGAAATTTGAGTTTGTTGAATCTCCGGTGATTGGTGGGTTTAGTGTCGGGAAGGTGCCGTCGGCTGGGGCGATTGCAGAGTTGTTTTCGGGGACTGAGGTTGCGGCGAGGACTAGGGTTCCGATTAGGGGACGTGCCGCGGTGAATTTTAGGTGGGGGGTTAGGGTTCCGGCTGAGGTTAAGGGCGAAAGCGCGTTTGGGAGGGTTCCGTTTCTTGTGATGGATAAAATTGGGGTGGAACATTTGCCAGCGGAGTGTGGGGATTTGAAGAATTTGAAGAAAGGAAAGGCGGAGAATGGATTGCTGAGGAATGCTGTGGAGGATCTTCGGAGAGAATTCACTAGTGTAAGGATTGGAGGAGGGAAAAGCTTTGAGGGGAGAAAGAATGAGAAGAAAACCGGGTCAGATTTTAGTGGTTATCCTGGGAAATCGACTGAGGCTGATGCGAGCAAGGAATTGGAGAAGGCGTTGAGGGGAGCCACCACTGTTGGAGCTTGA
Protein sequence

>Msa0386720

MSKSNQKTQSSILDVKGYAFPQNGGVLGNDDTKFEFVESPVIGGFSVGKVPSAGAIAELFSGTEVAARTRVPIRGRAAVNFRWGVRVPAEVKGESAFGRVPFLVMDKIGVEHLPAECGDLKNLKKGKAENGLLRNAVEDLRREFTSVRIGGGKSFEGRKNEKKTGSDFSGYPGKSTEADASKELEKALRGATTVGA*