Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399960 | AES69395.1 | 91.176 | 68 | 6 | 0 | 1 | 68 | 1 | 68 | 1.54e-33 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399960 | G7IZF1 | 91.176 | 68 | 6 | 0 | 1 | 68 | 1 | 68 | 7.34e-34 | 119 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0399960 | Msa1252000 | 0.801890 | 7.271596e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0399960 | MtrunA17_Chr3g0087981 | 91.176 | 68 | 6 | 0 | 1 | 68 | 1 | 68 | 1.41e-37 | 119 |
| Msa0399960 | MtrunA17_Chr3g0088031 | 81.967 | 61 | 11 | 0 | 8 | 68 | 1 | 61 | 7.44e-30 | 99.8 |
| Msa0399960 | MtrunA17_Chr3g0088011 | 75.000 | 68 | 15 | 1 | 1 | 68 | 1 | 66 | 5.73e-29 | 97.8 |
| Msa0399960 | MtrunA17_Chr3g0088251 | 77.941 | 68 | 13 | 1 | 1 | 68 | 1 | 66 | 1.18e-28 | 97.1 |
| Msa0399960 | MtrunA17_Chr3g0088021 | 75.000 | 68 | 15 | 1 | 1 | 68 | 1 | 66 | 6.55e-28 | 95.1 |
| Msa0399960 | MtrunA17_Chr3g0088071 | 80.882 | 68 | 11 | 1 | 1 | 68 | 1 | 66 | 1.23e-24 | 86.7 |
| Msa0399960 | MtrunA17_Chr3g0087931 | 70.149 | 67 | 18 | 1 | 2 | 68 | 3 | 67 | 6.42e-24 | 85.1 |
| Msa0399960 | MtrunA17_Chr3g0087921 | 71.212 | 66 | 17 | 1 | 3 | 68 | 4 | 67 | 1.58e-23 | 84.0 |
| Msa0399960 | MtrunA17_Chr3g0088041 | 88.636 | 44 | 5 | 0 | 25 | 68 | 23 | 66 | 6.78e-21 | 77.4 |
| Msa0399960 | MtrunA17_Chr3g0087911 | 66.667 | 66 | 20 | 1 | 3 | 68 | 4 | 67 | 2.86e-20 | 75.9 |
| Msa0399960 | MtrunA17_Chr3g0088081 | 75.000 | 64 | 16 | 0 | 5 | 68 | 2 | 65 | 6.33e-19 | 72.4 |
| Msa0399960 | MtrunA17_Chr3g0088271 | 70.312 | 64 | 19 | 0 | 5 | 68 | 2 | 65 | 1.66e-18 | 71.2 |
| Msa0399960 | MtrunA17_Chr3g0088001 | 75.000 | 68 | 16 | 1 | 1 | 68 | 1 | 67 | 2.38e-18 | 70.9 |
| Msa0399960 | MtrunA17_Chr3g0087941 | 79.545 | 44 | 9 | 0 | 25 | 68 | 25 | 68 | 2.33e-17 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCCTGATGCCGGAGCAGC+TGG | 0.400610 | 3_2:-23783683 | Msa0399960:CDS |
| GATCCAGCTGCTCCGGCATC+AGG | 0.412361 | 3_2:+23783680 | Msa0399970:CDS |
| CCCTCATTGTCTCCGGCACC+AGG | 0.418787 | 3_2:-23783704 | Msa0399960:CDS |
| GTCTTGAGCCGAGGCGATAG+AGG | 0.462001 | 3_2:+23783733 | Msa0399970:intron |
| GCTGCTCCGGCATCAGGACC+TGG | 0.469740 | 3_2:+23783686 | Msa0399970:CDS |
| TGTAACAGATCCAGCTGCTC+CGG | 0.486059 | 3_2:+23783673 | Msa0399970:CDS |
| CCGGCACCAGGTCCTGATGC+CGG | 0.497722 | 3_2:-23783692 | Msa0399960:CDS |
| CCGGCATCAGGACCTGGTGC+CGG | 0.506330 | 3_2:+23783692 | Msa0399970:CDS |
| CCGGAGACAATGAGGGGCTG+AGG | 0.541558 | 3_2:+23783711 | Msa0399970:CDS |
| ACCTGGTGCCGGAGACAATG+AGG | 0.555717 | 3_2:+23783703 | Msa0399970:CDS |
| ACAAATTCAGCGGCTATCAT+CGG | 0.555852 | 3_2:-23783653 | Msa0399960:CDS |
| GGCGATAGAGGCAGCCAACA+TGG | 0.557671 | 3_2:+23783745 | Msa0399970:intron |
| GTTGGCTGCCTCTATCGCCT+CGG | 0.577294 | 3_2:-23783741 | Msa0399960:CDS |
| CCTGGTGCCGGAGACAATGA+GGG | 0.578445 | 3_2:+23783704 | Msa0399970:CDS |
| CCTCAGCCCCTCATTGTCTC+CGG | 0.589131 | 3_2:-23783711 | Msa0399960:CDS |
| GGGGCTGAGGTCTTGAGCCG+AGG | 0.618003 | 3_2:+23783724 | Msa0399970:intron |
| CTGGTGCCGGAGACAATGAG+GGG | 0.652003 | 3_2:+23783705 | Msa0399970:CDS |
| TGGATCTGTTACAAATTCAG+CGG | 0.670408 | 3_2:-23783663 | Msa0399960:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGGATCTGTTACAAATTCAG+CGG | - | chr3_2:23783741-23783760 | Msa0399960:CDS | 35.0% | |
| ACAAATTCAGCGGCTATCAT+CGG | - | chr3_2:23783751-23783770 | Msa0399960:CDS | 40.0% | |
| CAACATGGCGACGAAAAAGA+AGG | + | chr3_2:23783647-23783666 | Msa0399970:CDS | 45.0% | |
| !! | CTTCTTTTTCGTCGCCATGT+TGG | - | chr3_2:23783645-23783664 | Msa0399960:CDS | 45.0% |
| TGTAACAGATCCAGCTGCTC+CGG | + | chr3_2:23783734-23783753 | Msa0399970:intron | 50.0% | |
| GGCGATAGAGGCAGCCAACA+TGG | + | chr3_2:23783662-23783681 | Msa0399970:CDS | 60.0% | |
| GTTGGCTGCCTCTATCGCCT+CGG | - | chr3_2:23783663-23783682 | Msa0399960:CDS | 60.0% | |
| GTCTTGAGCCGAGGCGATAG+AGG | + | chr3_2:23783674-23783693 | Msa0399970:CDS | 60.0% | |
| CCTCAGCCCCTCATTGTCTC+CGG | - | chr3_2:23783693-23783712 | Msa0399960:CDS | 60.0% | |
| ! | CTGGTGCCGGAGACAATGAG+GGG | + | chr3_2:23783702-23783721 | Msa0399970:CDS | 60.0% |
| ! | CCTGGTGCCGGAGACAATGA+GGG | + | chr3_2:23783703-23783722 | Msa0399970:CDS | 60.0% |
| ! | ACCTGGTGCCGGAGACAATG+AGG | + | chr3_2:23783704-23783723 | Msa0399970:CDS | 60.0% |
| CCGGAGACAATGAGGGGCTG+AGG | + | chr3_2:23783696-23783715 | Msa0399970:CDS | 65.0% | |
| CCCTCATTGTCTCCGGCACC+AGG | - | chr3_2:23783700-23783719 | Msa0399960:CDS | 65.0% | |
| GATCCAGCTGCTCCGGCATC+AGG | + | chr3_2:23783727-23783746 | Msa0399970:intron | 65.0% | |
| GGGGCTGAGGTCTTGAGCCG+AGG | + | chr3_2:23783683-23783702 | Msa0399970:CDS | 70.0% | |
| CCGGCATCAGGACCTGGTGC+CGG | + | chr3_2:23783715-23783734 | Msa0399970:intron | 70.0% | |
| CCGGCACCAGGTCCTGATGC+CGG | - | chr3_2:23783712-23783731 | Msa0399960:CDS | 70.0% | |
| GCTGCTCCGGCATCAGGACC+TGG | + | chr3_2:23783721-23783740 | Msa0399970:intron | 70.0% | |
| GGTCCTGATGCCGGAGCAGC+TGG | - | chr3_2:23783721-23783740 | Msa0399960:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 23783610 | 23783816 | 23783610 | ID=Msa0399960;Name=Msa0399960 |
| chr3_2 | mRNA | 23783610 | 23783816 | 23783610 | ID=Msa0399960-mRNA-1;Parent=Msa0399960;Name=Msa0399960-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|68 |
| chr3_2 | exon | 23783610 | 23783816 | 23783610 | ID=Msa0399960-mRNA-1:exon:5002;Parent=Msa0399960-mRNA-1 |
| chr3_2 | CDS | 23783610 | 23783816 | 23783610 | ID=Msa0399960-mRNA-1:cds;Parent=Msa0399960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
