Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0404810 | XP_013461729.1 | 94.690 | 339 | 15 | 1 | 1 | 336 | 1 | 339 | 0.0 | 661 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0404810 | sp|Q9FMH5|CCA31_ARATH | 52.450 | 347 | 148 | 3 | 3 | 337 | 12 | 353 | 2.03e-120 | 353 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0404810 | A0A072V166 | 94.690 | 339 | 15 | 1 | 1 | 336 | 1 | 339 | 0.0 | 661 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0140210 | Msa0404810 | 0.864306 | 1.336852e-64 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0404810 | MtrunA17_Chr3g0134401 | 94.690 | 339 | 15 | 1 | 1 | 336 | 1 | 339 | 0.0 | 661 |
| Msa0404810 | MtrunA17_Chr3g0134411 | 78.652 | 356 | 54 | 3 | 1 | 334 | 1 | 356 | 0.0 | 570 |
| Msa0404810 | MtrunA17_Chr3g0134421 | 67.039 | 358 | 89 | 6 | 1 | 336 | 1 | 351 | 6.97e-164 | 461 |
| Msa0404810 | MtrunA17_Chr3g0134441 | 54.227 | 343 | 133 | 6 | 1 | 334 | 2 | 329 | 3.87e-112 | 328 |
| Msa0404810 | MtrunA17_Chr1g0152871 | 57.971 | 276 | 110 | 3 | 65 | 334 | 82 | 357 | 9.16e-112 | 329 |
| Msa0404810 | MtrunA17_Chr1g0148681 | 48.649 | 259 | 129 | 2 | 74 | 328 | 156 | 414 | 2.09e-80 | 251 |
| Msa0404810 | MtrunA17_Chr3g0125041 | 47.328 | 262 | 133 | 2 | 74 | 330 | 243 | 504 | 4.23e-80 | 253 |
| Msa0404810 | MtrunA17_Chr3g0125051 | 45.788 | 273 | 145 | 1 | 65 | 334 | 231 | 503 | 2.00e-79 | 251 |
| Msa0404810 | MtrunA17_Chr3g0138431 | 46.241 | 266 | 140 | 1 | 65 | 327 | 204 | 469 | 4.16e-79 | 249 |
| Msa0404810 | MtrunA17_Chr3g0134431 | 51.751 | 257 | 96 | 5 | 66 | 316 | 1 | 235 | 3.77e-78 | 239 |
| Msa0404810 | MtrunA17_Chr2g0332181 | 43.015 | 272 | 147 | 2 | 63 | 326 | 151 | 422 | 8.69e-71 | 226 |
| Msa0404810 | MtrunA17_Chr2g0332171 | 44.944 | 267 | 142 | 2 | 65 | 326 | 212 | 478 | 3.80e-69 | 223 |
| Msa0404810 | MtrunA17_Chr8g0370671 | 34.263 | 251 | 155 | 4 | 74 | 321 | 176 | 419 | 1.24e-43 | 155 |
| Msa0404810 | MtrunA17_Chr7g0255771 | 34.510 | 255 | 160 | 5 | 74 | 324 | 199 | 450 | 3.68e-43 | 155 |
| Msa0404810 | MtrunA17_Chr5g0406741 | 33.068 | 251 | 158 | 4 | 74 | 321 | 172 | 415 | 1.49e-40 | 147 |
| Msa0404810 | MtrunA17_Chr5g0441371 | 33.745 | 243 | 150 | 6 | 74 | 312 | 165 | 400 | 5.78e-40 | 145 |
| Msa0404810 | MtrunA17_Chr8g0357001 | 35.616 | 219 | 129 | 5 | 74 | 288 | 486 | 696 | 1.23e-35 | 137 |
| Msa0404810 | MtrunA17_Chr3g0102861 | 31.188 | 202 | 115 | 8 | 106 | 293 | 49 | 240 | 2.36e-19 | 86.7 |
| Msa0404810 | MtrunA17_Chr3g0102851 | 29.060 | 234 | 138 | 10 | 107 | 322 | 60 | 283 | 3.46e-19 | 86.3 |
| Msa0404810 | MtrunA17_Chr3g0103471 | 31.188 | 202 | 115 | 8 | 106 | 293 | 49 | 240 | 6.67e-19 | 85.5 |
| Msa0404810 | MtrunA17_Chr3g0102621 | 30.097 | 206 | 118 | 9 | 103 | 293 | 52 | 246 | 3.20e-17 | 80.5 |
| Msa0404810 | MtrunA17_Chr3g0102631 | 31.319 | 182 | 107 | 7 | 125 | 295 | 1 | 175 | 3.34e-15 | 73.6 |
| Msa0404810 | MtrunA17_Chr3g0102651 | 31.319 | 182 | 107 | 7 | 125 | 295 | 1 | 175 | 3.34e-15 | 73.6 |
| Msa0404810 | MtrunA17_Chr3g0102661 | 31.319 | 182 | 107 | 7 | 125 | 295 | 1 | 175 | 3.34e-15 | 73.6 |
| Msa0404810 | MtrunA17_Chr3g0102671 | 31.319 | 182 | 107 | 7 | 125 | 295 | 1 | 175 | 3.34e-15 | 73.6 |
| Msa0404810 | MtrunA17_Chr6g0462911 | 31.156 | 199 | 121 | 8 | 103 | 296 | 70 | 257 | 6.38e-15 | 74.3 |
| Msa0404810 | MtrunA17_Chr5g0418901 | 37.975 | 79 | 46 | 1 | 129 | 204 | 7 | 85 | 2.48e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0404810 | AT5G43080.1 | 52.450 | 347 | 148 | 3 | 3 | 337 | 12 | 353 | 2.06e-121 | 353 |
| Msa0404810 | AT5G43080.2 | 51.585 | 347 | 146 | 4 | 3 | 337 | 12 | 348 | 8.33e-118 | 344 |
| Msa0404810 | AT1G47230.1 | 47.740 | 354 | 165 | 5 | 3 | 337 | 11 | 363 | 4.02e-108 | 320 |
| Msa0404810 | AT1G47230.2 | 47.606 | 355 | 165 | 6 | 3 | 337 | 11 | 364 | 2.40e-106 | 316 |
| Msa0404810 | AT1G47210.2 | 46.978 | 364 | 160 | 6 | 3 | 337 | 11 | 370 | 1.15e-105 | 314 |
| Msa0404810 | AT1G47220.1 | 54.275 | 269 | 118 | 2 | 74 | 337 | 56 | 324 | 6.60e-97 | 290 |
| Msa0404810 | AT1G44110.1 | 48.864 | 264 | 132 | 1 | 74 | 334 | 194 | 457 | 1.01e-80 | 253 |
| Msa0404810 | AT5G11300.1 | 45.652 | 276 | 142 | 2 | 66 | 333 | 159 | 434 | 1.58e-77 | 244 |
| Msa0404810 | AT1G80370.1 | 46.415 | 265 | 138 | 2 | 74 | 335 | 197 | 460 | 2.48e-75 | 239 |
| Msa0404810 | AT5G25380.3 | 44.487 | 263 | 143 | 1 | 74 | 333 | 173 | 435 | 8.82e-75 | 237 |
| Msa0404810 | AT5G25380.1 | 44.487 | 263 | 143 | 1 | 74 | 333 | 173 | 435 | 8.82e-75 | 237 |
| Msa0404810 | AT5G25380.4 | 44.487 | 263 | 143 | 1 | 74 | 333 | 179 | 441 | 1.07e-74 | 237 |
| Msa0404810 | AT1G15570.1 | 45.703 | 256 | 136 | 1 | 74 | 326 | 188 | 443 | 1.80e-72 | 231 |
| Msa0404810 | AT1G77390.1 | 41.971 | 274 | 156 | 1 | 65 | 335 | 169 | 442 | 4.95e-72 | 230 |
| Msa0404810 | AT5G25380.2 | 44.715 | 246 | 133 | 1 | 74 | 316 | 173 | 418 | 1.53e-69 | 223 |
| Msa0404810 | AT5G11300.2 | 46.781 | 233 | 107 | 3 | 66 | 281 | 159 | 391 | 8.62e-64 | 208 |
| Msa0404810 | AT3G11520.1 | 36.434 | 258 | 152 | 6 | 74 | 328 | 159 | 407 | 7.62e-42 | 150 |
| Msa0404810 | AT5G06150.1 | 35.857 | 251 | 150 | 5 | 74 | 321 | 182 | 424 | 3.64e-41 | 149 |
| Msa0404810 | AT4G37490.1 | 35.769 | 260 | 150 | 7 | 74 | 324 | 165 | 416 | 4.78e-41 | 149 |
| Msa0404810 | AT1G20610.1 | 33.466 | 251 | 157 | 4 | 74 | 321 | 177 | 420 | 4.89e-40 | 146 |
| Msa0404810 | AT1G76310.1 | 35.039 | 254 | 154 | 5 | 74 | 323 | 177 | 423 | 7.26e-40 | 145 |
| Msa0404810 | AT3G11520.2 | 38.393 | 224 | 127 | 5 | 74 | 294 | 159 | 374 | 9.73e-40 | 144 |
| Msa0404810 | AT2G17620.1 | 36.981 | 265 | 157 | 4 | 60 | 321 | 157 | 414 | 1.56e-38 | 142 |
| Msa0404810 | AT4G35620.1 | 36.508 | 252 | 150 | 4 | 74 | 322 | 172 | 416 | 2.34e-38 | 141 |
| Msa0404810 | AT2G26760.1 | 37.109 | 256 | 150 | 6 | 74 | 325 | 130 | 378 | 1.29e-37 | 139 |
| Msa0404810 | AT1G16330.1 | 32.510 | 243 | 152 | 5 | 74 | 312 | 395 | 629 | 4.99e-37 | 140 |
| Msa0404810 | AT1G76310.3 | 31.206 | 282 | 155 | 5 | 74 | 323 | 177 | 451 | 3.43e-35 | 133 |
| Msa0404810 | AT1G76310.2 | 35.945 | 217 | 128 | 5 | 74 | 286 | 177 | 386 | 4.07e-34 | 129 |
| Msa0404810 | AT4G35620.2 | 39.614 | 207 | 114 | 5 | 74 | 277 | 172 | 370 | 9.84e-34 | 128 |
| Msa0404810 | AT1G47210.1 | 42.473 | 186 | 77 | 5 | 3 | 162 | 11 | 192 | 3.98e-32 | 119 |
| Msa0404810 | AT5G06150.2 | 41.958 | 143 | 78 | 3 | 74 | 213 | 182 | 322 | 1.31e-26 | 108 |
| Msa0404810 | AT1G76310.4 | 38.562 | 153 | 89 | 3 | 74 | 222 | 177 | 328 | 2.53e-24 | 102 |
| Msa0404810 | AT1G20590.1 | 36.478 | 159 | 90 | 5 | 136 | 290 | 66 | 217 | 4.13e-23 | 97.1 |
| Msa0404810 | AT1G14750.2 | 29.231 | 195 | 116 | 5 | 81 | 260 | 185 | 372 | 1.32e-12 | 68.6 |
| Msa0404810 | AT1G14750.3 | 29.231 | 195 | 116 | 5 | 81 | 260 | 353 | 540 | 3.81e-12 | 67.8 |
| Msa0404810 | AT1G14750.1 | 29.231 | 195 | 116 | 5 | 81 | 260 | 353 | 540 | 4.56e-12 | 67.4 |
| Msa0404810 | AT5G67260.1 | 26.697 | 221 | 149 | 6 | 82 | 293 | 72 | 288 | 2.15e-11 | 64.7 |
| Msa0404810 | AT5G67260.2 | 26.697 | 221 | 149 | 6 | 82 | 293 | 72 | 288 | 2.75e-11 | 64.7 |
Find 105 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTGCCCATTTCAAAATTT+AGG | 0.157914 | 3_2:-37535371 | None:intergenic |
| ACCTTTCCTTAATTCCAATT+TGG | 0.169676 | 3_2:+37534751 | Msa0404810:CDS |
| ACAGAGAGATGAAGGGTTTC+TGG | 0.207212 | 3_2:-37534938 | None:intergenic |
| CAAACGACAGAGGGTTGTTT+TGG | 0.227942 | 3_2:+37534628 | Msa0404810:CDS |
| GCTTATCCAAATTGGAATTA+AGG | 0.235094 | 3_2:-37534757 | None:intergenic |
| TTTAGAAATGTGATGACATT+AGG | 0.241622 | 3_2:-37535393 | None:intergenic |
| TACATAAGGCTTATCCAAAT+TGG | 0.252861 | 3_2:-37534765 | None:intergenic |
| ACTTCTATAAAGTAATAATT+TGG | 0.267667 | 3_2:-37536143 | None:intergenic |
| GTTGCCCATTTCAAAATTTA+GGG | 0.281705 | 3_2:-37535370 | None:intergenic |
| AAGACCAATGGTTGGTTATA+TGG | 0.298772 | 3_2:+37534829 | Msa0404810:CDS |
| TGAGATTGATTTGTTGAGAT+TGG | 0.300594 | 3_2:-37534723 | None:intergenic |
| TGTACATTGGAGTTTCTGTT+TGG | 0.309368 | 3_2:-37534693 | None:intergenic |
| AAAAGTTAGCAGGCAGTAAC+TGG | 0.312506 | 3_2:-37536289 | None:intergenic |
| TGAAGCTTAAATTTAATGAT+AGG | 0.315288 | 3_2:-37534992 | None:intergenic |
| CAGATATTCAAACTGCAAAT+TGG | 0.319844 | 3_2:-37535646 | None:intergenic |
| TCCGGGGTCTTGTCTTTGAT+AGG | 0.326598 | 3_2:+37536388 | Msa0404810:three_prime_UTR |
| GTGAAGAGGTGACAACAAAA+AGG | 0.329986 | 3_2:-37536596 | None:intergenic |
| TTCATCCCTTTGTGAATGCT+TGG | 0.338640 | 3_2:+37535883 | Msa0404810:CDS |
| GTTAATACAACCTATGCTTC+CGG | 0.343292 | 3_2:+37536370 | Msa0404810:three_prime_UTR |
| CAGCATCCTTCAAAGCTGTT+AGG | 0.347762 | 3_2:+37535972 | Msa0404810:CDS |
| CAAAACAACCCTCTGTCGTT+TGG | 0.351711 | 3_2:-37534627 | None:intergenic |
| CATAGGTGTTATCAGTGATT+TGG | 0.355291 | 3_2:-37535186 | None:intergenic |
| TTAGAAATGTGATGACATTA+GGG | 0.359501 | 3_2:-37535392 | None:intergenic |
| TCCGGCCTGGAGTGCATCCT+TGG | 0.367775 | 3_2:+37535769 | Msa0404810:CDS |
| TGAGAGGGACATTGGTTGAT+TGG | 0.379637 | 3_2:+37534882 | Msa0404810:CDS |
| TTAATACAACCTATGCTTCC+GGG | 0.381399 | 3_2:+37536371 | Msa0404810:three_prime_UTR |
| AAAACAACCCTCTGTCGTTT+GGG | 0.384728 | 3_2:-37534626 | None:intergenic |
| TGGCAGAAATCTATAGCCTT+TGG | 0.389420 | 3_2:-37535166 | None:intergenic |
| TCATCCCTTTGTGAATGCTT+GGG | 0.393250 | 3_2:+37535884 | Msa0404810:CDS |
| AAGTAATAATTTGGTATCTC+TGG | 0.395913 | 3_2:-37536134 | None:intergenic |
| TTTCTCCCTAACAGCTTTGA+AGG | 0.397121 | 3_2:-37535978 | None:intergenic |
| GTATCTCTGGCGGGGAAGGC+AGG | 0.399107 | 3_2:-37536121 | None:intergenic |
| TTGGGTTGATAATCAAAATC+AGG | 0.403192 | 3_2:-37534674 | None:intergenic |
| GAGATTGATTTGTTGAGATT+GGG | 0.413711 | 3_2:-37534722 | None:intergenic |
| GGGTTACAAGTCGTCTGAAT+TGG | 0.414268 | 3_2:+37535904 | Msa0404810:CDS |
| GATGAAGGGTTTCTGGAAGA+AGG | 0.417673 | 3_2:-37534931 | None:intergenic |
| GGTACAAATTAAGATAAAGT+TGG | 0.418145 | 3_2:-37536574 | None:intergenic |
| GTACATTGGAGTTTCTGTTT+GGG | 0.433143 | 3_2:-37534692 | None:intergenic |
| TATGCTTGAGCTAATTAAGT+TGG | 0.436585 | 3_2:-37536253 | None:intergenic |
| TATTACCTCGTCAAGATCAT+AGG | 0.437323 | 3_2:-37535203 | None:intergenic |
| TTCTTGCTAGATTTATTCTC+CGG | 0.441267 | 3_2:+37535751 | Msa0404810:CDS |
| GCTTAAATTTAATGATAGGT+TGG | 0.445907 | 3_2:-37534988 | None:intergenic |
| AAATTTAAGCTTCAGTTGCT+TGG | 0.466396 | 3_2:+37535001 | Msa0404810:CDS |
| GTACAAATTAAGATAAAGTT+GGG | 0.467294 | 3_2:-37536573 | None:intergenic |
| AAGATTCTTGCCTTCTGTTG+TGG | 0.472652 | 3_2:+37535713 | Msa0404810:CDS |
| ATGACTTGTATTTGTCAAGA+AGG | 0.473884 | 3_2:+37535948 | Msa0404810:CDS |
| TTATACATTCATAGTCCAAC+AGG | 0.477447 | 3_2:-37535692 | None:intergenic |
| TGGTGGTTGTTGATTGATGT+TGG | 0.477583 | 3_2:-37534591 | None:intergenic |
| CCGGCCTGGAGTGCATCCTT+GGG | 0.477863 | 3_2:+37535770 | Msa0404810:CDS |
| ATTGGTTGATTGGTTAGTTG+AGG | 0.479448 | 3_2:+37534892 | Msa0404810:CDS |
| AATAATTTGGTATCTCTGGC+GGG | 0.480876 | 3_2:-37536130 | None:intergenic |
| AACTTGAACACAAAGGAACT+TGG | 0.481303 | 3_2:-37536340 | None:intergenic |
| TGTATGAAACAGAGAGATGA+AGG | 0.484873 | 3_2:-37534946 | None:intergenic |
| TCGAATATGTCGGATACATA+AGG | 0.485282 | 3_2:-37534779 | None:intergenic |
| CCGGGGTCTTGTCTTTGATA+GGG | 0.489875 | 3_2:+37536389 | Msa0404810:three_prime_UTR |
| AAATCAGGAAGATTGAGATC+TGG | 0.497094 | 3_2:-37534659 | None:intergenic |
| TTTGGTATCTCTGGCGGGGA+AGG | 0.505867 | 3_2:-37536125 | None:intergenic |
| GATTATCATCAAAAGTTAGC+AGG | 0.506166 | 3_2:-37536299 | None:intergenic |
| CAGAAATCTATAGCCTTTGG+TGG | 0.507750 | 3_2:-37535163 | None:intergenic |
| AATTGGAATTAAGGAAAGGT+AGG | 0.510771 | 3_2:-37534748 | None:intergenic |
| GCTAGATTTATTCTCCGGCC+TGG | 0.514323 | 3_2:+37535756 | Msa0404810:CDS |
| TTTACCCAAGGATGCACTCC+AGG | 0.524136 | 3_2:-37535774 | None:intergenic |
| GGAGAAATACAAGCAACAAA+AGG | 0.524411 | 3_2:+37535994 | Msa0404810:CDS |
| AGACAAGACCCCGGAAGCAT+AGG | 0.528334 | 3_2:-37536380 | None:intergenic |
| GATTGCTTCTGAGAATCAAA+AGG | 0.529405 | 3_2:+37535531 | Msa0404810:CDS |
| ATCAAACATGAGAGGGACAT+TGG | 0.531177 | 3_2:+37534874 | Msa0404810:CDS |
| AGAAATCTATAGCCTTTGGT+GGG | 0.532149 | 3_2:-37535162 | None:intergenic |
| TCTGGAACAGTTCAAATATG+TGG | 0.532832 | 3_2:+37536094 | Msa0404810:intron |
| AGCATCCTTCAAAGCTGTTA+GGG | 0.534414 | 3_2:+37535973 | Msa0404810:CDS |
| TGACTTGTATTTGTCAAGAA+GGG | 0.539254 | 3_2:+37535949 | Msa0404810:CDS |
| TCCAAATTGGAATTAAGGAA+AGG | 0.539850 | 3_2:-37534752 | None:intergenic |
| AATACAAGACTCTGCCTCCA+TGG | 0.545034 | 3_2:+37536542 | Msa0404810:three_prime_UTR |
| CCCAAGGATGCACTCCAGGC+CGG | 0.547889 | 3_2:-37535770 | None:intergenic |
| ACAGAAGCAGCCACAACAGA+AGG | 0.558857 | 3_2:-37535723 | None:intergenic |
| ATTGGAATTAAGGAAAGGTA+GGG | 0.562882 | 3_2:-37534747 | None:intergenic |
| TAATAATTTGGTATCTCTGG+CGG | 0.563787 | 3_2:-37536131 | None:intergenic |
| TCAACAACCACCACATGTGA+TGG | 0.572935 | 3_2:+37534601 | Msa0404810:CDS |
| GTAACCCAAGCATTCACAAA+GGG | 0.587375 | 3_2:-37535888 | None:intergenic |
| ATCTTACGATGCAATGAGCA+TGG | 0.601202 | 3_2:-37535032 | None:intergenic |
| AAAGTTGGGTGTTTCAACCA+TGG | 0.604483 | 3_2:-37536559 | None:intergenic |
| ATAATTTGGTATCTCTGGCG+GGG | 0.610149 | 3_2:-37536129 | None:intergenic |
| ATTCGATTATCTTCGAACGA+TGG | 0.615413 | 3_2:+37534796 | Msa0404810:CDS |
| GCATAGGTTGTATTAACAAG+TGG | 0.616545 | 3_2:-37536364 | None:intergenic |
| TGAAGAAATAACCCCACCAA+AGG | 0.619552 | 3_2:+37535150 | Msa0404810:CDS |
| TTCTCCATATAACCAACCAT+TGG | 0.621997 | 3_2:-37534833 | None:intergenic |
| ATATCTGTGCAACTATCTTG+CGG | 0.626935 | 3_2:+37535662 | Msa0404810:CDS |
| GCATTACATCAAACATGAGA+GGG | 0.627034 | 3_2:+37534867 | Msa0404810:CDS |
| CTGATGCAGTGTGAATAACT+TGG | 0.628967 | 3_2:+37536166 | Msa0404810:exon |
| GGCATTACATCAAACATGAG+AGG | 0.629142 | 3_2:+37534866 | Msa0404810:CDS |
| GAAATCTATAGCCTTTGGTG+GGG | 0.630025 | 3_2:-37535161 | None:intergenic |
| GATGGTTCCCAAACGACAGA+GGG | 0.630488 | 3_2:+37534619 | Msa0404810:CDS |
| TAATACAACCTATGCTTCCG+GGG | 0.632552 | 3_2:+37536372 | Msa0404810:three_prime_UTR |
| TCGAAGATAATCGAATATGT+CGG | 0.642977 | 3_2:-37534789 | None:intergenic |
| TCTTGCGGATATATGCCTGT+TGG | 0.646330 | 3_2:+37535677 | Msa0404810:CDS |
| TTGGGAACCATCACATGTGG+TGG | 0.654688 | 3_2:-37534608 | None:intergenic |
| ACTTGGTGAATGACTGAATG+TGG | 0.670026 | 3_2:+37536183 | Msa0404810:three_prime_UTR |
| TGATGGTTCCCAAACGACAG+AGG | 0.670654 | 3_2:+37534618 | Msa0404810:CDS |
| TATATGGAGAATGTTCAAAG+AGG | 0.675074 | 3_2:+37534845 | Msa0404810:CDS |
| GTATGAAACAGAGAGATGAA+GGG | 0.679453 | 3_2:-37534945 | None:intergenic |
| CCCTATCAAAGACAAGACCC+CGG | 0.686231 | 3_2:-37536389 | None:intergenic |
| GTTGGGTGTTTCAACCATGG+AGG | 0.704267 | 3_2:-37536556 | None:intergenic |
| CGTTTGGGAACCATCACATG+TGG | 0.706491 | 3_2:-37534611 | None:intergenic |
| TGTAACCCAAGCATTCACAA+AGG | 0.709657 | 3_2:-37535889 | None:intergenic |
| AAGTGGCAACTTGAACACAA+AGG | 0.719724 | 3_2:-37536347 | None:intergenic |
| TAACACCTATGATCTTGACG+AGG | 0.759007 | 3_2:+37535198 | Msa0404810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATAGTATTCTTTTATT+TGG | + | chr3_2:37535221-37535240 | Msa0404810:intron | 10.0% |
| !! | TATAAAAGAATAATATTTCA+AGG | + | chr3_2:37535832-37535851 | Msa0404810:intron | 10.0% |
| !!! | TTCGATTTTACAATAAAAAA+AGG | - | chr3_2:37535426-37535445 | None:intergenic | 15.0% |
| !! | ATTGTAAAATCGAATATATA+AGG | + | chr3_2:37535432-37535451 | Msa0404810:intron | 15.0% |
| !! | ACTTCTATAAAGTAATAATT+TGG | - | chr3_2:37536146-37536165 | None:intergenic | 15.0% |
| !!! | TTTATTGTTTATGATTTTTG+TGG | + | chr3_2:37536447-37536466 | Msa0404810:three_prime_UTR | 15.0% |
| !! | TGAAGCTTAAATTTAATGAT+AGG | - | chr3_2:37534995-37535014 | None:intergenic | 20.0% |
| !! | TTTAATTCCATTTAATATGC+AGG | + | chr3_2:37535122-37535141 | Msa0404810:intron | 20.0% |
| !!! | TATCAAATTTTGTTCTTGAT+TGG | + | chr3_2:37535249-37535268 | Msa0404810:intron | 20.0% |
| !! | CAATTAAATTCATTTCAACA+AGG | - | chr3_2:37535458-37535477 | None:intergenic | 20.0% |
| !! | CTTGTTGAAATGAATTTAAT+TGG | + | chr3_2:37535456-37535475 | Msa0404810:intron | 20.0% |
| !!! | ATGACTGACAAATATATTTT+TGG | + | chr3_2:37536209-37536228 | Msa0404810:three_prime_UTR | 20.0% |
| !! | ATTGCAAAAATAATAGTCAA+TGG | - | chr3_2:37536518-37536537 | None:intergenic | 20.0% |
| !! | GTACAAATTAAGATAAAGTT+GGG | - | chr3_2:37536576-37536595 | None:intergenic | 20.0% |
| ! | GCTTAAATTTAATGATAGGT+TGG | - | chr3_2:37534991-37535010 | None:intergenic | 25.0% |
| !!! | AAGACCCTAAATTTTGAAAT+GGG | + | chr3_2:37535366-37535385 | Msa0404810:CDS | 25.0% |
| ! | TTAGAAATGTGATGACATTA+GGG | - | chr3_2:37535395-37535414 | None:intergenic | 25.0% |
| ! | TTTAGAAATGTGATGACATT+AGG | - | chr3_2:37535396-37535415 | None:intergenic | 25.0% |
| !!! | AATGCTGTTTTATTAATAGC+TGG | + | chr3_2:37535562-37535581 | Msa0404810:intron | 25.0% |
| ! | CAATTAGATAACACAATAAC+AGG | - | chr3_2:37535599-37535618 | None:intergenic | 25.0% |
| ! | TAACATCAAATATTTACCCA+AGG | - | chr3_2:37535789-37535808 | None:intergenic | 25.0% |
| ! | AAGTAATAATTTGGTATCTC+TGG | - | chr3_2:37536137-37536156 | None:intergenic | 25.0% |
| !!! | ACTGACAAATATATTTTTGG+TGG | + | chr3_2:37536212-37536231 | Msa0404810:three_prime_UTR | 25.0% |
| ! | GGTACAAATTAAGATAAAGT+TGG | - | chr3_2:37536577-37536596 | None:intergenic | 25.0% |
| !! | TTGGGTTGATAATCAAAATC+AGG | - | chr3_2:37534677-37534696 | None:intergenic | 30.0% |
| !!! | GATTGGGATTTTTTGTACAT+TGG | - | chr3_2:37534709-37534728 | None:intergenic | 30.0% |
| !! | GAGATTGATTTGTTGAGATT+GGG | - | chr3_2:37534725-37534744 | None:intergenic | 30.0% |
| !! | TGAGATTGATTTGTTGAGAT+TGG | - | chr3_2:37534726-37534745 | None:intergenic | 30.0% |
| ATTGGAATTAAGGAAAGGTA+GGG | - | chr3_2:37534750-37534769 | None:intergenic | 30.0% | |
| AATTGGAATTAAGGAAAGGT+AGG | - | chr3_2:37534751-37534770 | None:intergenic | 30.0% | |
| ACCTTTCCTTAATTCCAATT+TGG | + | chr3_2:37534751-37534770 | Msa0404810:CDS | 30.0% | |
| TCCAAATTGGAATTAAGGAA+AGG | - | chr3_2:37534755-37534774 | None:intergenic | 30.0% | |
| GCTTATCCAAATTGGAATTA+AGG | - | chr3_2:37534760-37534779 | None:intergenic | 30.0% | |
| TACATAAGGCTTATCCAAAT+TGG | - | chr3_2:37534768-37534787 | None:intergenic | 30.0% | |
| TCGAAGATAATCGAATATGT+CGG | - | chr3_2:37534792-37534811 | None:intergenic | 30.0% | |
| TATATGGAGAATGTTCAAAG+AGG | + | chr3_2:37534845-37534864 | Msa0404810:CDS | 30.0% | |
| AAATTTAAGCTTCAGTTGCT+TGG | + | chr3_2:37535001-37535020 | Msa0404810:CDS | 30.0% | |
| CATACTTCCTGCATATTAAA+TGG | - | chr3_2:37535132-37535151 | None:intergenic | 30.0% | |
| ATGTATGCTGTTGTTAATGT+TGG | + | chr3_2:37535317-37535336 | Msa0404810:intron | 30.0% | |
| !!! | GTTAATGTTGGTTTTGAAGA+TGG | + | chr3_2:37535329-37535348 | Msa0404810:intron | 30.0% |
| !! | CAAGACCCTAAATTTTGAAA+TGG | + | chr3_2:37535365-37535384 | Msa0404810:CDS | 30.0% |
| GTTGCCCATTTCAAAATTTA+GGG | - | chr3_2:37535373-37535392 | None:intergenic | 30.0% | |
| !! | ATTCATTTCAGGAGTTTTGT+TGG | + | chr3_2:37535508-37535527 | Msa0404810:intron | 30.0% |
| !!! | TTCATTTCAGGAGTTTTGTT+GGG | + | chr3_2:37535509-37535528 | Msa0404810:intron | 30.0% |
| CAGATATTCAAACTGCAAAT+TGG | - | chr3_2:37535649-37535668 | None:intergenic | 30.0% | |
| TTATACATTCATAGTCCAAC+AGG | - | chr3_2:37535695-37535714 | None:intergenic | 30.0% | |
| TTCTTGCTAGATTTATTCTC+CGG | + | chr3_2:37535751-37535770 | Msa0404810:CDS | 30.0% | |
| ! | ATGACTTGTATTTGTCAAGA+AGG | + | chr3_2:37535948-37535967 | Msa0404810:CDS | 30.0% |
| ! | TGACTTGTATTTGTCAAGAA+GGG | + | chr3_2:37535949-37535968 | Msa0404810:CDS | 30.0% |
| TAATAATTTGGTATCTCTGG+CGG | - | chr3_2:37536134-37536153 | None:intergenic | 30.0% | |
| !!! | ATATATTTTTGGTGGCTATG+CGG | + | chr3_2:37536220-37536239 | Msa0404810:three_prime_UTR | 30.0% |
| TATGCTTGAGCTAATTAAGT+TGG | - | chr3_2:37536256-37536275 | None:intergenic | 30.0% | |
| GATTATCATCAAAAGTTAGC+AGG | - | chr3_2:37536302-37536321 | None:intergenic | 30.0% | |
| AAATCAGGAAGATTGAGATC+TGG | - | chr3_2:37534662-37534681 | None:intergenic | 35.0% | |
| GTACATTGGAGTTTCTGTTT+GGG | - | chr3_2:37534695-37534714 | None:intergenic | 35.0% | |
| TGTACATTGGAGTTTCTGTT+TGG | - | chr3_2:37534696-37534715 | None:intergenic | 35.0% | |
| TCGAATATGTCGGATACATA+AGG | - | chr3_2:37534782-37534801 | None:intergenic | 35.0% | |
| ATTCGATTATCTTCGAACGA+TGG | + | chr3_2:37534796-37534815 | Msa0404810:CDS | 35.0% | |
| AAAAAGAGAAGACCAATGGT+TGG | + | chr3_2:37534821-37534840 | Msa0404810:CDS | 35.0% | |
| ! | AAGACCAATGGTTGGTTATA+TGG | + | chr3_2:37534829-37534848 | Msa0404810:CDS | 35.0% |
| TTCTCCATATAACCAACCAT+TGG | - | chr3_2:37534836-37534855 | None:intergenic | 35.0% | |
| GCATTACATCAAACATGAGA+GGG | + | chr3_2:37534867-37534886 | Msa0404810:CDS | 35.0% | |
| ! | ATTGGTTGATTGGTTAGTTG+AGG | + | chr3_2:37534892-37534911 | Msa0404810:CDS | 35.0% |
| GTATGAAACAGAGAGATGAA+GGG | - | chr3_2:37534948-37534967 | None:intergenic | 35.0% | |
| TGTATGAAACAGAGAGATGA+AGG | - | chr3_2:37534949-37534968 | None:intergenic | 35.0% | |
| AGAAATCTATAGCCTTTGGT+GGG | - | chr3_2:37535165-37535184 | None:intergenic | 35.0% | |
| ! | CATAGGTGTTATCAGTGATT+TGG | - | chr3_2:37535189-37535208 | None:intergenic | 35.0% |
| TATTACCTCGTCAAGATCAT+AGG | - | chr3_2:37535206-37535225 | None:intergenic | 35.0% | |
| GGTTGCCCATTTCAAAATTT+AGG | - | chr3_2:37535374-37535393 | None:intergenic | 35.0% | |
| ! | GATTGCTTCTGAGAATCAAA+AGG | + | chr3_2:37535531-37535550 | Msa0404810:CDS | 35.0% |
| ATATCTGTGCAACTATCTTG+CGG | + | chr3_2:37535662-37535681 | Msa0404810:CDS | 35.0% | |
| GGAGAAATACAAGCAACAAA+AGG | + | chr3_2:37535994-37536013 | Msa0404810:CDS | 35.0% | |
| !! | ATTGACTTGAACTCATTGTC+TGG | + | chr3_2:37536076-37536095 | Msa0404810:intron | 35.0% |
| ! | TCTGGAACAGTTCAAATATG+TGG | + | chr3_2:37536094-37536113 | Msa0404810:intron | 35.0% |
| AATAATTTGGTATCTCTGGC+GGG | - | chr3_2:37536133-37536152 | None:intergenic | 35.0% | |
| AACTTGAACACAAAGGAACT+TGG | - | chr3_2:37536343-37536362 | None:intergenic | 35.0% | |
| GCATAGGTTGTATTAACAAG+TGG | - | chr3_2:37536367-37536386 | None:intergenic | 35.0% | |
| GTTAATACAACCTATGCTTC+CGG | + | chr3_2:37536370-37536389 | Msa0404810:three_prime_UTR | 35.0% | |
| TTAATACAACCTATGCTTCC+GGG | + | chr3_2:37536371-37536390 | Msa0404810:three_prime_UTR | 35.0% | |
| ! | TGGTGGTTGTTGATTGATGT+TGG | - | chr3_2:37534594-37534613 | None:intergenic | 40.0% |
| ! | AAAACAACCCTCTGTCGTTT+GGG | - | chr3_2:37534629-37534648 | None:intergenic | 40.0% |
| !! | AAACGACAGAGGGTTGTTTT+GGG | + | chr3_2:37534629-37534648 | Msa0404810:CDS | 40.0% |
| GGAGAAAAAGAGAAGACCAA+TGG | + | chr3_2:37534817-37534836 | Msa0404810:CDS | 40.0% | |
| GGCATTACATCAAACATGAG+AGG | + | chr3_2:37534866-37534885 | Msa0404810:CDS | 40.0% | |
| ATCAAACATGAGAGGGACAT+TGG | + | chr3_2:37534874-37534893 | Msa0404810:CDS | 40.0% | |
| ATCTTACGATGCAATGAGCA+TGG | - | chr3_2:37535035-37535054 | None:intergenic | 40.0% | |
| TGAAGAAATAACCCCACCAA+AGG | + | chr3_2:37535150-37535169 | Msa0404810:CDS | 40.0% | |
| GAAATCTATAGCCTTTGGTG+GGG | - | chr3_2:37535164-37535183 | None:intergenic | 40.0% | |
| CAGAAATCTATAGCCTTTGG+TGG | - | chr3_2:37535166-37535185 | None:intergenic | 40.0% | |
| TGGCAGAAATCTATAGCCTT+TGG | - | chr3_2:37535169-37535188 | None:intergenic | 40.0% | |
| TAACACCTATGATCTTGACG+AGG | + | chr3_2:37535198-37535217 | Msa0404810:CDS | 40.0% | |
| !! | CACTTGCTGTGATTCATTTC+AGG | + | chr3_2:37535497-37535516 | Msa0404810:intron | 40.0% |
| AAGATTCTTGCCTTCTGTTG+TGG | + | chr3_2:37535713-37535732 | Msa0404810:CDS | 40.0% | |
| TTCATCCCTTTGTGAATGCT+TGG | + | chr3_2:37535883-37535902 | Msa0404810:CDS | 40.0% | |
| TCATCCCTTTGTGAATGCTT+GGG | + | chr3_2:37535884-37535903 | Msa0404810:CDS | 40.0% | |
| GTAACCCAAGCATTCACAAA+GGG | - | chr3_2:37535891-37535910 | None:intergenic | 40.0% | |
| TGTAACCCAAGCATTCACAA+AGG | - | chr3_2:37535892-37535911 | None:intergenic | 40.0% | |
| AGCATCCTTCAAAGCTGTTA+GGG | + | chr3_2:37535973-37535992 | Msa0404810:CDS | 40.0% | |
| ! | TTTCTCCCTAACAGCTTTGA+AGG | - | chr3_2:37535981-37536000 | None:intergenic | 40.0% |
| ATAATTTGGTATCTCTGGCG+GGG | - | chr3_2:37536132-37536151 | None:intergenic | 40.0% | |
| CTGATGCAGTGTGAATAACT+TGG | + | chr3_2:37536166-37536185 | Msa0404810:exon | 40.0% | |
| ! | ACTTGGTGAATGACTGAATG+TGG | + | chr3_2:37536183-37536202 | Msa0404810:three_prime_UTR | 40.0% |
| AAAAGTTAGCAGGCAGTAAC+TGG | - | chr3_2:37536292-37536311 | None:intergenic | 40.0% | |
| AAGTGGCAACTTGAACACAA+AGG | - | chr3_2:37536350-37536369 | None:intergenic | 40.0% | |
| TAATACAACCTATGCTTCCG+GGG | + | chr3_2:37536372-37536391 | Msa0404810:three_prime_UTR | 40.0% | |
| AAAGTTGGGTGTTTCAACCA+TGG | - | chr3_2:37536562-37536581 | None:intergenic | 40.0% | |
| TCAACAACCACCACATGTGA+TGG | + | chr3_2:37534601-37534620 | Msa0404810:CDS | 45.0% | |
| CAAAACAACCCTCTGTCGTT+TGG | - | chr3_2:37534630-37534649 | None:intergenic | 45.0% | |
| ! | CAAACGACAGAGGGTTGTTT+TGG | + | chr3_2:37534628-37534647 | Msa0404810:CDS | 45.0% |
| ! | TGAGAGGGACATTGGTTGAT+TGG | + | chr3_2:37534882-37534901 | Msa0404810:CDS | 45.0% |
| ! | GATGAAGGGTTTCTGGAAGA+AGG | - | chr3_2:37534934-37534953 | None:intergenic | 45.0% |
| ACAGAGAGATGAAGGGTTTC+TGG | - | chr3_2:37534941-37534960 | None:intergenic | 45.0% | |
| TCTTGCGGATATATGCCTGT+TGG | + | chr3_2:37535677-37535696 | Msa0404810:CDS | 45.0% | |
| GGGTTACAAGTCGTCTGAAT+TGG | + | chr3_2:37535904-37535923 | Msa0404810:CDS | 45.0% | |
| CAGCATCCTTCAAAGCTGTT+AGG | + | chr3_2:37535972-37535991 | Msa0404810:CDS | 45.0% | |
| AATACAAGACTCTGCCTCCA+TGG | + | chr3_2:37536542-37536561 | Msa0404810:three_prime_UTR | 45.0% | |
| TTGGGAACCATCACATGTGG+TGG | - | chr3_2:37534611-37534630 | None:intergenic | 50.0% | |
| CGTTTGGGAACCATCACATG+TGG | - | chr3_2:37534614-37534633 | None:intergenic | 50.0% | |
| TGATGGTTCCCAAACGACAG+AGG | + | chr3_2:37534618-37534637 | Msa0404810:CDS | 50.0% | |
| GATGGTTCCCAAACGACAGA+GGG | + | chr3_2:37534619-37534638 | Msa0404810:CDS | 50.0% | |
| ACAGAAGCAGCCACAACAGA+AGG | - | chr3_2:37535726-37535745 | None:intergenic | 50.0% | |
| GCTAGATTTATTCTCCGGCC+TGG | + | chr3_2:37535756-37535775 | Msa0404810:CDS | 50.0% | |
| TTTACCCAAGGATGCACTCC+AGG | - | chr3_2:37535777-37535796 | None:intergenic | 50.0% | |
| ! | TCCGGGGTCTTGTCTTTGAT+AGG | + | chr3_2:37536388-37536407 | Msa0404810:three_prime_UTR | 50.0% |
| CCCTATCAAAGACAAGACCC+CGG | - | chr3_2:37536392-37536411 | None:intergenic | 50.0% | |
| ! | CCGGGGTCTTGTCTTTGATA+GGG | + | chr3_2:37536389-37536408 | Msa0404810:three_prime_UTR | 50.0% |
| GTTGGGTGTTTCAACCATGG+AGG | - | chr3_2:37536559-37536578 | None:intergenic | 50.0% | |
| TTTGGTATCTCTGGCGGGGA+AGG | - | chr3_2:37536128-37536147 | None:intergenic | 55.0% | |
| ! | AGACAAGACCCCGGAAGCAT+AGG | - | chr3_2:37536383-37536402 | None:intergenic | 55.0% |
| TCCGGCCTGGAGTGCATCCT+TGG | + | chr3_2:37535769-37535788 | Msa0404810:CDS | 65.0% | |
| CCCAAGGATGCACTCCAGGC+CGG | - | chr3_2:37535773-37535792 | None:intergenic | 65.0% | |
| CCGGCCTGGAGTGCATCCTT+GGG | + | chr3_2:37535770-37535789 | Msa0404810:CDS | 65.0% | |
| GTATCTCTGGCGGGGAAGGC+AGG | - | chr3_2:37536124-37536143 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 37534563 | 37536603 | 37534563 | ID=Msa0404810;Name=Msa0404810 |
| chr3_2 | mRNA | 37534563 | 37536603 | 37534563 | ID=Msa0404810-mRNA-1;Parent=Msa0404810;Name=Msa0404810-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0.33|0.28|1|1|1|7|434|337 |
| chr3_2 | exon | 37534563 | 37535047 | 37534563 | ID=Msa0404810-mRNA-1:exon:7493;Parent=Msa0404810-mRNA-1 |
| chr3_2 | exon | 37535144 | 37535219 | 37535144 | ID=Msa0404810-mRNA-1:exon:7494;Parent=Msa0404810-mRNA-1 |
| chr3_2 | exon | 37535348 | 37535415 | 37535348 | ID=Msa0404810-mRNA-1:exon:7495;Parent=Msa0404810-mRNA-1 |
| chr3_2 | exon | 37535519 | 37535552 | 37535519 | ID=Msa0404810-mRNA-1:exon:7496;Parent=Msa0404810-mRNA-1 |
| chr3_2 | exon | 37535642 | 37535791 | 37535642 | ID=Msa0404810-mRNA-1:exon:7497;Parent=Msa0404810-mRNA-1 |
| chr3_2 | exon | 37535881 | 37536015 | 37535881 | ID=Msa0404810-mRNA-1:exon:7498;Parent=Msa0404810-mRNA-1 |
| chr3_2 | exon | 37536104 | 37536603 | 37536104 | ID=Msa0404810-mRNA-1:exon:7499;Parent=Msa0404810-mRNA-1 |
| chr3_2 | CDS | 37534563 | 37535047 | 37534563 | ID=Msa0404810-mRNA-1:cds;Parent=Msa0404810-mRNA-1 |
| chr3_2 | CDS | 37535144 | 37535219 | 37535144 | ID=Msa0404810-mRNA-1:cds;Parent=Msa0404810-mRNA-1 |
| chr3_2 | CDS | 37535348 | 37535415 | 37535348 | ID=Msa0404810-mRNA-1:cds;Parent=Msa0404810-mRNA-1 |
| chr3_2 | CDS | 37535519 | 37535552 | 37535519 | ID=Msa0404810-mRNA-1:cds;Parent=Msa0404810-mRNA-1 |
| chr3_2 | CDS | 37535642 | 37535791 | 37535642 | ID=Msa0404810-mRNA-1:cds;Parent=Msa0404810-mRNA-1 |
| chr3_2 | CDS | 37535881 | 37536015 | 37535881 | ID=Msa0404810-mRNA-1:cds;Parent=Msa0404810-mRNA-1 |
| chr3_2 | CDS | 37536104 | 37536169 | 37536104 | ID=Msa0404810-mRNA-1:cds;Parent=Msa0404810-mRNA-1 |
| chr3_2 | three_prime_UTR | 37536170 | 37536603 | 37536170 | ID=Msa0404810-mRNA-1:three_prime_utr;Parent=Msa0404810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
