Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0409790 | Msa0409800 | 0.816458 | 5.473993e-52 | -8.615850e-47 |
| Msa0409800 | Msa0454530 | 0.805861 | 1.088087e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAAGAGTTCATTATTAA+TGG | 0.179856 | 3_2:-50965932 | Msa0409800:CDS |
| CATTATTAATGGAGATATAT+TGG | 0.278214 | 3_2:-50965921 | Msa0409800:CDS |
| GATCCCCGTCCTCCTCCTCC+TGG | 0.403019 | 3_2:-50965775 | Msa0409800:CDS |
| ACATCCAGGAGGAGGAGGAC+GGG | 0.436653 | 3_2:+50965771 | None:intergenic |
| CATCCAGGAGGAGGAGGACG+GGG | 0.450422 | 3_2:+50965772 | None:intergenic |
| GTTGAACATCCAGGAGGAGG+AGG | 0.500520 | 3_2:+50965766 | None:intergenic |
| TGTGATCTTTACAGAACAGA+AGG | 0.532542 | 3_2:-50965797 | Msa0409800:intron |
| ATCAGATCTATGGAAGTTGA+AGG | 0.542810 | 3_2:-50965977 | None:intergenic |
| TGTTCAACTTCTTCATCCTG+TGG | 0.543465 | 3_2:-50965751 | Msa0409800:CDS |
| AACATCCAGGAGGAGGAGGA+CGG | 0.552128 | 3_2:+50965770 | None:intergenic |
| GAAGTTGAACATCCAGGAGG+AGG | 0.553310 | 3_2:+50965763 | None:intergenic |
| GATGAAGTCGAAAATTCCAC+AGG | 0.569404 | 3_2:+50965735 | None:intergenic |
| TACACTTTATTTGATGACGA+CGG | 0.579533 | 3_2:+50965701 | None:intergenic |
| GTAGATGAAGACGACAGAGA+TGG | 0.603274 | 3_2:-50965893 | Msa0409800:CDS |
| GATGAAGAAGTTGAACATCC+AGG | 0.630010 | 3_2:+50965757 | None:intergenic |
| GAAGAAGTTGAACATCCAGG+AGG | 0.753924 | 3_2:+50965760 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAAGAGTTCATTATTAA+TGG | - | chr3_2:50965743-50965762 | Msa0409800:CDS | 20.0% |
| !! | CATTATTAATGGAGATATAT+TGG | - | chr3_2:50965754-50965773 | Msa0409800:CDS | 20.0% |
| ! | CACATAAATCATAATCATCA+AGG | + | chr3_2:50965862-50965881 | None:intergenic | 25.0% |
| ACAGAGATGGAATCAAAAAG+AGG | - | chr3_2:50965795-50965814 | Msa0409800:intron | 35.0% | |
| CAGAGATGGAATCAAAAAGA+GGG | - | chr3_2:50965796-50965815 | Msa0409800:intron | 35.0% | |
| ! | GATAGAAGCAACTTTTGTGA+TGG | - | chr3_2:50965829-50965848 | Msa0409800:intron | 35.0% |
| TGTGATCTTTACAGAACAGA+AGG | - | chr3_2:50965878-50965897 | Msa0409800:CDS | 35.0% | |
| GATGAAGAAGTTGAACATCC+AGG | + | chr3_2:50965921-50965940 | None:intergenic | 40.0% | |
| TGTTCAACTTCTTCATCCTG+TGG | - | chr3_2:50965924-50965943 | Msa0409800:CDS | 40.0% | |
| GATGAAGTCGAAAATTCCAC+AGG | + | chr3_2:50965943-50965962 | None:intergenic | 40.0% | |
| GTAGATGAAGACGACAGAGA+TGG | - | chr3_2:50965782-50965801 | Msa0409800:CDS | 45.0% | |
| GAAGAAGTTGAACATCCAGG+AGG | + | chr3_2:50965918-50965937 | None:intergenic | 45.0% | |
| GAAGTTGAACATCCAGGAGG+AGG | + | chr3_2:50965915-50965934 | None:intergenic | 50.0% | |
| AACATCCAGGAGGAGGAGGA+CGG | + | chr3_2:50965908-50965927 | None:intergenic | 55.0% | |
| GTTGAACATCCAGGAGGAGG+AGG | + | chr3_2:50965912-50965931 | None:intergenic | 55.0% | |
| ACATCCAGGAGGAGGAGGAC+GGG | + | chr3_2:50965907-50965926 | None:intergenic | 60.0% | |
| CATCCAGGAGGAGGAGGACG+GGG | + | chr3_2:50965906-50965925 | None:intergenic | 65.0% | |
| GATCCCCGTCCTCCTCCTCC+TGG | - | chr3_2:50965900-50965919 | Msa0409800:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 50965707 | 50965990 | 50965707 | ID=Msa0409800;Name=Msa0409800 |
| chr3_2 | mRNA | 50965707 | 50965990 | 50965707 | ID=Msa0409800-mRNA-1;Parent=Msa0409800;Name=Msa0409800-mRNA-1;_AED=0.48;_eAED=0.69;_QI=0|0|0|1|0|0|2|0|82 |
| chr3_2 | exon | 50965841 | 50965990 | 50965841 | ID=Msa0409800-mRNA-1:exon:10075;Parent=Msa0409800-mRNA-1 |
| chr3_2 | exon | 50965707 | 50965805 | 50965707 | ID=Msa0409800-mRNA-1:exon:10074;Parent=Msa0409800-mRNA-1 |
| chr3_2 | CDS | 50965841 | 50965990 | 50965841 | ID=Msa0409800-mRNA-1:cds;Parent=Msa0409800-mRNA-1 |
| chr3_2 | CDS | 50965707 | 50965805 | 50965707 | ID=Msa0409800-mRNA-1:cds;Parent=Msa0409800-mRNA-1 |
| Gene Sequence |
| Protein sequence |
