AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0424840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0424840 MtrunA17_Chr3g0126721 98.221 281 5 0 1 281 1 281 0.0 571
Msa0424840 MtrunA17_Chr1g0147111 72.028 286 70 5 1 280 1 282 4.86e-143 403
Msa0424840 MtrunA17_Chr8g0387241 49.412 85 41 1 192 274 185 269 6.37e-20 87.8
Msa0424840 MtrunA17_Chr4g0030421 45.783 83 41 2 192 274 1 79 1.84e-16 74.3
Msa0424840 MtrunA17_Chr1g0168401 42.268 97 42 2 192 274 267 363 9.94e-16 76.6
Msa0424840 MtrunA17_Chr5g0397361 41.573 89 48 1 192 280 139 223 2.87e-15 73.6
Msa0424840 MtrunA17_Chr1g0186171 65.306 49 17 0 226 274 312 360 3.57e-15 75.1
Msa0424840 MtrunA17_Chr2g0327621 57.692 52 22 0 226 277 151 202 1.26e-14 72.0
Msa0424840 MtrunA17_Chr2g0311591 58.000 50 21 0 226 275 166 215 1.21e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0424840 AT5G53420.1 49.291 282 115 10 1 274 1 262 7.56e-82 247
Msa0424840 AT4G27900.1 49.458 277 118 9 1 274 1 258 2.52e-80 243
Msa0424840 AT4G27900.2 49.458 277 118 9 1 274 1 258 2.52e-80 243
Msa0424840 AT5G53420.2 54.229 201 67 8 81 274 1 183 1.66e-59 187
Msa0424840 AT5G53420.5 54.229 201 67 8 81 274 1 183 1.66e-59 187
Msa0424840 AT5G53420.4 54.229 201 67 8 81 274 1 183 1.66e-59 187
Msa0424840 AT5G53420.3 41.364 220 106 8 1 212 1 205 2.52e-45 152
Msa0424840 AT1G63820.1 45.918 98 38 1 192 274 139 236 4.37e-19 85.5
Msa0424840 AT5G41380.1 47.253 91 36 2 192 274 173 259 5.41e-18 82.4
Msa0424840 AT1G04500.4 67.347 49 16 0 226 274 284 332 4.84e-16 77.8
Msa0424840 AT1G04500.1 67.347 49 16 0 226 274 284 332 4.84e-16 77.8
Msa0424840 AT1G04500.3 67.347 49 16 0 226 274 275 323 5.53e-16 77.4
Msa0424840 AT2G33350.2 41.000 100 42 2 192 274 255 354 6.54e-16 77.4
Msa0424840 AT2G33350.4 41.000 100 42 2 192 274 244 343 8.63e-16 77.0
Msa0424840 AT2G33350.5 41.000 100 42 2 192 274 264 363 8.89e-16 77.0
Msa0424840 AT2G33350.1 50.667 75 27 1 200 274 291 355 1.47e-15 76.6
Msa0424840 AT5G59990.1 59.091 44 18 0 237 280 170 213 8.00e-12 64.3
Msa0424840 AT3G12890.1 56.000 50 22 0 226 275 135 184 2.39e-11 62.8

Find 87 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTGACATCTACTATATT+TGG 0.186667 3_2:-75118704 None:intergenic
TAGTAGATGTCAAATTTCTT+AGG 0.205610 3_2:+75118711 Msa0424840:three_prime_UTR
AAGAAGACAAAGAGGAATTT+TGG 0.219086 3_2:+75118059 Msa0424840:CDS
CCATTGAAGCATTGAACTTC+TGG 0.228558 3_2:-75115932 None:intergenic
GAGTAAATAGAGCATAAATT+TGG 0.274696 3_2:-75115672 None:intergenic
AAATCTTATCTGCTGTATTA+AGG 0.276251 3_2:+75118742 Msa0424840:three_prime_UTR
GTATATGTTTATGTATATAT+TGG 0.278119 3_2:+75119005 Msa0424840:three_prime_UTR
CTAGCTCTAGATGTGTAAAT+AGG 0.284846 3_2:+75118802 Msa0424840:three_prime_UTR
GGATGTTACTTAGAGGAGTT+TGG 0.295892 3_2:-75115649 None:intergenic
AGTAAATAGAGCATAAATTT+GGG 0.299515 3_2:-75115671 None:intergenic
CTCCTCACTGCTGCAGTTAC+TGG 0.304798 3_2:-75118006 None:intergenic
GCAGAAATGTATAGAGAAAA+TGG 0.320082 3_2:+75115837 Msa0424840:exon
CTAACGCTCTTCGGTAATAA+TGG 0.351082 3_2:-75117621 None:intergenic
ACGTAATATGTGCTTGGAAT+TGG 0.366084 3_2:+75118620 Msa0424840:three_prime_UTR
TTCGGTAATAATGGCATATC+AGG 0.371338 3_2:-75117612 None:intergenic
AGAAACAAACTTGAATTGCT+TGG 0.373109 3_2:+75118978 Msa0424840:three_prime_UTR
GACTCTTCAATCATCGGTTC+AGG 0.379136 3_2:-75117351 None:intergenic
TGTATTCTATGATTGCAAAA+AGG 0.379871 3_2:+75117482 Msa0424840:CDS
GTTGGTTTCAGTATGCTTGC+AGG 0.384533 3_2:+75118449 Msa0424840:intron
GCAAATCTTCCACGGACTCT+TGG 0.408686 3_2:-75118493 None:intergenic
CCTGCTTTCCTTGGTATCTC+AGG 0.411336 3_2:+75117696 Msa0424840:CDS
AATTTGGGGATGTTACTTAG+AGG 0.416097 3_2:-75115656 None:intergenic
GAAGTTTAAGTTCGATGGAT+TGG 0.417320 3_2:+75117649 Msa0424840:CDS
CTCAGGAAGTTTAAGTTCGA+TGG 0.421168 3_2:+75117644 Msa0424840:CDS
ATTTAGTGAAGGAGATATAA+AGG 0.423299 3_2:+75117755 Msa0424840:CDS
ACGGCCTCTCCACGGGAGAT+TGG 0.431556 3_2:-75117977 None:intergenic
CTTCCTGAGCTAACGCTCTT+CGG 0.432530 3_2:-75117630 None:intergenic
AGTAGATGTCAAATTTCTTA+GGG 0.445486 3_2:+75118712 Msa0424840:three_prime_UTR
GCAATGAAGCCTGCTTTCCT+TGG 0.445883 3_2:+75117687 Msa0424840:CDS
AAAGTACAAAGTGATGAGTT+AGG 0.456409 3_2:-75115753 None:intergenic
TTCTAGAACACTCTTAAACT+TGG 0.457064 3_2:-75115801 None:intergenic
CTCCTGTGTGTGTCCACACT+TGG 0.465873 3_2:+75118649 Msa0424840:three_prime_UTR
CATTGAGATGGCTGCTGTCA+TGG 0.466318 3_2:-75117391 None:intergenic
AAAGAATGAAGAATCTGATC+AGG 0.470069 3_2:+75118516 Msa0424840:CDS
CTCTGAGATTCTAGAAATCA+AGG 0.483324 3_2:+75117539 Msa0424840:CDS
CATTCTTTGCAAATCTTCCA+CGG 0.485735 3_2:-75118501 None:intergenic
CTGTGTGTGTCCACACTTGG+TGG 0.489691 3_2:+75118652 Msa0424840:three_prime_UTR
AGTTCGATGGATTGGATGCA+TGG 0.490181 3_2:+75117657 Msa0424840:CDS
TACAAAAGCTCTCTAGATAC+AGG 0.491032 3_2:+75118033 Msa0424840:CDS
CCAGAAGTTCAATGCTTCAA+TGG 0.493713 3_2:+75115932 Msa0424840:CDS
TGCTCAACGTAATATGTGCT+TGG 0.496245 3_2:+75118614 Msa0424840:three_prime_UTR
AGAATCTGATCAGGCAAAGA+AGG 0.498079 3_2:+75118525 Msa0424840:CDS
TCTATTTGAAAAGGCGGGAA+TGG 0.501622 3_2:+75117506 Msa0424840:CDS
AGGTTTGTTAAATAAGAGAA+GGG 0.508962 3_2:+75118822 Msa0424840:three_prime_UTR
TAGGTTTGTTAAATAAGAGA+AGG 0.514288 3_2:+75118821 Msa0424840:three_prime_UTR
CTTTGTCACATAAAGCCACT+TGG 0.516207 3_2:-75118572 None:intergenic
CAAACTTGAATTGCTTGGAC+TGG 0.520047 3_2:+75118983 Msa0424840:three_prime_UTR
TGGCTGCTGTCATGGGATCA+AGG 0.522632 3_2:-75117383 None:intergenic
TTCTCGTCAATGTTCAGTAA+TGG 0.526652 3_2:-75117564 None:intergenic
TCATATACATGAACATAGGA+TGG 0.527341 3_2:-75118860 None:intergenic
TTTACACATCTAGAGCTAGA+AGG 0.531389 3_2:-75118798 None:intergenic
CCTGAGATACCAAGGAAAGC+AGG 0.532865 3_2:-75117696 None:intergenic
ATGCCTGACTCTTCAATCAT+CGG 0.544939 3_2:-75117357 None:intergenic
AAGGATCTATTTGAAAAGGC+GGG 0.547844 3_2:+75117501 Msa0424840:CDS
GAACCGATGATTGAAGAGTC+AGG 0.548760 3_2:+75117354 Msa0424840:CDS
ATTGAGATGGCTGCTGTCAT+GGG 0.551127 3_2:-75117390 None:intergenic
TTACCGAAGAGCGTTAGCTC+AGG 0.557410 3_2:+75117627 Msa0424840:CDS
CATATACATGAACATAGGAT+GGG 0.564600 3_2:-75118859 None:intergenic
TTCTGCTGCAAAATCATACT+CGG 0.567600 3_2:-75117310 None:intergenic
GTTTCAGTATGCTTGCAGGA+AGG 0.571787 3_2:+75118453 Msa0424840:intron
TACAACTCCTCCACCAAGTG+TGG 0.572297 3_2:-75118662 None:intergenic
AATAATGGCATATCAGGAAG+TGG 0.576682 3_2:-75117606 None:intergenic
TTGATCATATACATGAACAT+AGG 0.579147 3_2:-75118864 None:intergenic
GTTTGTTAAATAAGAGAAGG+GGG 0.585840 3_2:+75118824 Msa0424840:three_prime_UTR
TGTGTGTCCACACTTGGTGG+AGG 0.587105 3_2:+75118655 Msa0424840:three_prime_UTR
ACAGGAACAAGAAGACAAAG+AGG 0.587806 3_2:+75118051 Msa0424840:CDS
ATATACATGAACATAGGATG+GGG 0.592699 3_2:-75118858 None:intergenic
GCCACAAGATATCATTGAGA+TGG 0.597336 3_2:-75117403 None:intergenic
AAAGGATCTATTTGAAAAGG+CGG 0.598414 3_2:+75117500 Msa0424840:CDS
TGTTATCACGTTTAACCAAG+TGG 0.599140 3_2:+75118557 Msa0424840:three_prime_UTR
TGTATTAAGGTCTCTGTAGA+CGG 0.600820 3_2:+75118755 Msa0424840:three_prime_UTR
GGTTTGTTAAATAAGAGAAG+GGG 0.603967 3_2:+75118823 Msa0424840:three_prime_UTR
GACAGTCAACCAAGAGTCCG+TGG 0.610591 3_2:+75118484 Msa0424840:CDS
AATCTATTCCTGAGATACCA+AGG 0.616072 3_2:-75117704 None:intergenic
GCCATCTCAATGATATCTTG+TGG 0.620490 3_2:+75117402 Msa0424840:CDS
GTAAATAGAGCATAAATTTG+GGG 0.625116 3_2:-75115670 None:intergenic
CACCAAGTGTGGACACACAC+AGG 0.629888 3_2:-75118651 None:intergenic
TTGTCCAATCTCCCGTGGAG+AGG 0.631289 3_2:+75117973 Msa0424840:CDS
TTGAAGAATAGAAATATCTG+TGG 0.633420 3_2:-75117439 None:intergenic
ATGCGAAGATCATTTAGTGA+AGG 0.636958 3_2:+75117744 Msa0424840:CDS
GAACGTTGTCCAATCTCCCG+TGG 0.640756 3_2:+75117968 Msa0424840:CDS
AATTGTGTAGATAAGACCAT+GGG 0.640817 3_2:-75115869 None:intergenic
ATTTCTAGAATCTCAGAGAG+CGG 0.654213 3_2:-75117534 None:intergenic
ACATTGCAGAAAACTGAACA+AGG 0.668264 3_2:-75117287 None:intergenic
AAATTGTGTAGATAAGACCA+TGG 0.672601 3_2:-75115870 None:intergenic
TACCAGTAACTGCAGCAGTG+AGG 0.694981 3_2:+75118004 Msa0424840:CDS
TATACATGAACATAGGATGG+GGG 0.702492 3_2:-75118857 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAATTGAGCATAAA+AGG + chr3_2:75116290-75116309 Msa0424840:intron 15.0%
!! AAAAAAAATTGAGCATAAAA+GGG + chr3_2:75116291-75116310 Msa0424840:intron 15.0%
!!! ATTTTCTATCCTTATTTTAA+AGG - chr3_2:75116477-75116496 None:intergenic 15.0%
!! TTAAAAGATGTAATAAAACA+TGG - chr3_2:75117211-75117230 None:intergenic 15.0%
!!! TTTTTTTTTTTTTGTGTTTG+TGG + chr3_2:75117242-75117261 Msa0424840:intron 15.0%
!! TCAGTTTATAGTATAAATTA+TGG + chr3_2:75117834-75117853 Msa0424840:intron 15.0%
!! GTATATGTTTATGTATATAT+TGG + chr3_2:75119005-75119024 Msa0424840:three_prime_UTR 15.0%
!! AGTAAATAGAGCATAAATTT+GGG - chr3_2:75115674-75115693 None:intergenic 20.0%
!! TCAGAAAAGAAAAAACAAAA+TGG - chr3_2:75115699-75115718 None:intergenic 20.0%
!!! TATAAGTTTTATTCCTTGTT+TGG + chr3_2:75116080-75116099 Msa0424840:intron 20.0%
!! GAAACTCATAAATAAATAGA+AGG - chr3_2:75116358-75116377 None:intergenic 20.0%
!!! ATAAAAAAAGTGTAGAGAAA+AGG + chr3_2:75116495-75116514 Msa0424840:intron 20.0%
!! AATTATAGATTGTTGTAGAA+AGG + chr3_2:75116545-75116564 Msa0424840:intron 20.0%
!! ACTTTCATCATTAATTTAAC+AGG - chr3_2:75116588-75116607 None:intergenic 20.0%
!! TGTTAAATTAATGATGAAAG+TGG + chr3_2:75116587-75116606 Msa0424840:intron 20.0%
!!! TTTTTATTCATGTGAAGAAA+GGG - chr3_2:75118314-75118333 None:intergenic 20.0%
!!! TTTACCAAATAACATTTTCA+AGG - chr3_2:75118338-75118357 None:intergenic 20.0%
!! AATCATCATATGAATATCAA+CGG - chr3_2:75118426-75118445 None:intergenic 20.0%
!! TTGATATTCATATGATGATT+TGG + chr3_2:75118426-75118445 Msa0424840:intron 20.0%
!! TGATATTCATATGATGATTT+GGG + chr3_2:75118427-75118446 Msa0424840:intron 20.0%
!! AATTTGACATCTACTATATT+TGG - chr3_2:75118707-75118726 None:intergenic 20.0%
!!! TTTTTCAAGATACTGTAATT+TGG - chr3_2:75118941-75118960 None:intergenic 20.0%
! GTAAATAGAGCATAAATTTG+GGG - chr3_2:75115673-75115692 None:intergenic 25.0%
! GAGTAAATAGAGCATAAATT+TGG - chr3_2:75115675-75115694 None:intergenic 25.0%
!!! AATTTTGAAACTTCACAGTT+TGG + chr3_2:75116013-75116032 Msa0424840:intron 25.0%
!!! TTCTATCCTTATTTTAAAGG+AGG - chr3_2:75116474-75116493 None:intergenic 25.0%
! TCAAAATACTTAAACTGCAA+AGG - chr3_2:75116750-75116769 None:intergenic 25.0%
!!! AATATTGTTTTGTGCTTTTG+TGG + chr3_2:75116862-75116881 Msa0424840:intron 25.0%
! TCATCAAAACAGAATAGAAT+AGG - chr3_2:75116897-75116916 None:intergenic 25.0%
! TAAGAAATGTGTAAATTCTC+AGG - chr3_2:75117009-75117028 None:intergenic 25.0%
! AGAAGAAAAGAAAAACAAGT+AGG - chr3_2:75117134-75117153 None:intergenic 25.0%
! TTGAAGAATAGAAATATCTG+TGG - chr3_2:75117442-75117461 None:intergenic 25.0%
! TGTATTCTATGATTGCAAAA+AGG + chr3_2:75117482-75117501 Msa0424840:CDS 25.0%
! CAAAAAGGATCTATTTGAAA+AGG + chr3_2:75117497-75117516 Msa0424840:CDS 25.0%
! ATTTAGTGAAGGAGATATAA+AGG + chr3_2:75117755-75117774 Msa0424840:CDS 25.0%
!!! ACAAACAGTTTCTACTTTTA+AGG + chr3_2:75117873-75117892 Msa0424840:intron 25.0%
!! CTTTTTATTCATGTGAAGAA+AGG - chr3_2:75118315-75118334 None:intergenic 25.0%
! AAAGCCTTGAAAATGTTATT+TGG + chr3_2:75118331-75118350 Msa0424840:intron 25.0%
! TAGTAGATGTCAAATTTCTT+AGG + chr3_2:75118711-75118730 Msa0424840:three_prime_UTR 25.0%
! AGTAGATGTCAAATTTCTTA+GGG + chr3_2:75118712-75118731 Msa0424840:three_prime_UTR 25.0%
! AAATCTTATCTGCTGTATTA+AGG + chr3_2:75118742-75118761 Msa0424840:three_prime_UTR 25.0%
!!! TAGGTTTGTTAAATAAGAGA+AGG + chr3_2:75118821-75118840 Msa0424840:three_prime_UTR 25.0%
!!! AGGTTTGTTAAATAAGAGAA+GGG + chr3_2:75118822-75118841 Msa0424840:three_prime_UTR 25.0%
! TTGATCATATACATGAACAT+AGG - chr3_2:75118867-75118886 None:intergenic 25.0%
AAAGTACAAAGTGATGAGTT+AGG - chr3_2:75115756-75115775 None:intergenic 30.0%
!! TTCTAGAACACTCTTAAACT+TGG - chr3_2:75115804-75115823 None:intergenic 30.0%
GCAGAAATGTATAGAGAAAA+TGG + chr3_2:75115837-75115856 Msa0424840:exon 30.0%
AATTGTGTAGATAAGACCAT+GGG - chr3_2:75115872-75115891 None:intergenic 30.0%
AAATTGTGTAGATAAGACCA+TGG - chr3_2:75115873-75115892 None:intergenic 30.0%
TATCTCACCATTTCTTTGAT+GGG + chr3_2:75115971-75115990 Msa0424840:intron 30.0%
TGAAAAACCCATCAAAGAAA+TGG - chr3_2:75115981-75116000 None:intergenic 30.0%
!!! AAGTTTTATTCCTTGTTTGG+TGG + chr3_2:75116083-75116102 Msa0424840:intron 30.0%
ATATTATCAACCACCAAACA+AGG - chr3_2:75116096-75116115 None:intergenic 30.0%
AAGAAAACATTCAGACAGAA+AGG - chr3_2:75116194-75116213 None:intergenic 30.0%
AGAAGGAAGATTATACAACA+CGG - chr3_2:75116341-75116360 None:intergenic 30.0%
AAAGTGTAGAGAAAAGGATT+TGG + chr3_2:75116501-75116520 Msa0424840:intron 30.0%
TCATTGACAATTGCAAATGA+TGG + chr3_2:75116610-75116629 Msa0424840:intron 30.0%
!! AATTGCAAATGATGGTTTTG+AGG + chr3_2:75116618-75116637 Msa0424840:intron 30.0%
! ACTAATCCACCTTCTAATTT+TGG + chr3_2:75116665-75116684 Msa0424840:intron 30.0%
TTCCTTTGACCAAAATTAGA+AGG - chr3_2:75116677-75116696 None:intergenic 30.0%
CAGTACATATCAAGAAGAAA+AGG - chr3_2:75116950-75116969 None:intergenic 30.0%
TCTTCTTGATATGTACTGTT+TGG + chr3_2:75116952-75116971 Msa0424840:intron 30.0%
AGAAAAGAAAAACAAGTAGG+AGG - chr3_2:75117131-75117150 None:intergenic 30.0%
ATAAAACATGGCTTATCAGT+GGG - chr3_2:75117199-75117218 None:intergenic 30.0%
AATAAAACATGGCTTATCAG+TGG - chr3_2:75117200-75117219 None:intergenic 30.0%
AAAGGATCTATTTGAAAAGG+CGG + chr3_2:75117500-75117519 Msa0424840:CDS 30.0%
!! ATAGATTTTGATGCGGTTTA+TGG + chr3_2:75117720-75117739 Msa0424840:CDS 30.0%
! ATCTTTTCACATCCAGAAAT+CGG + chr3_2:75117796-75117815 Msa0424840:intron 30.0%
ATAAATTATGGATCCTTGTG+TGG + chr3_2:75117846-75117865 Msa0424840:intron 30.0%
AATGAGAGGAATAAATACCT+AGG - chr3_2:75117903-75117922 None:intergenic 30.0%
AGGTATTTATTCCTCTCATT+CGG + chr3_2:75117903-75117922 Msa0424840:intron 30.0%
! AAGAAGACAAAGAGGAATTT+TGG + chr3_2:75118059-75118078 Msa0424840:CDS 30.0%
!! GAATTTTGGAAGGAAAATCA+AGG + chr3_2:75118073-75118092 Msa0424840:CDS 30.0%
TTTCACATGAATAAAAAGCG+AGG + chr3_2:75118136-75118155 Msa0424840:intron 30.0%
TTCACATGAATAAAAAGCGA+GGG + chr3_2:75118137-75118156 Msa0424840:intron 30.0%
! TCAAAAGTAGGTAATGGTTT+AGG - chr3_2:75118382-75118401 None:intergenic 30.0%
! ATTCATATGATGATTTGGGT+TGG + chr3_2:75118431-75118450 Msa0424840:intron 30.0%
AAAGAATGAAGAATCTGATC+AGG + chr3_2:75118516-75118535 Msa0424840:CDS 30.0%
!! GGTTTGTTAAATAAGAGAAG+GGG + chr3_2:75118823-75118842 Msa0424840:three_prime_UTR 30.0%
! GTTTGTTAAATAAGAGAAGG+GGG + chr3_2:75118824-75118843 Msa0424840:three_prime_UTR 30.0%
ATATACATGAACATAGGATG+GGG - chr3_2:75118861-75118880 None:intergenic 30.0%
CATATACATGAACATAGGAT+GGG - chr3_2:75118862-75118881 None:intergenic 30.0%
TCATATACATGAACATAGGA+TGG - chr3_2:75118863-75118882 None:intergenic 30.0%
ACAGTATCTTGAAAAATGCT+AGG + chr3_2:75118945-75118964 Msa0424840:three_prime_UTR 30.0%
AGAAACAAACTTGAATTGCT+TGG + chr3_2:75118978-75118997 Msa0424840:three_prime_UTR 30.0%
! AATTTGGGGATGTTACTTAG+AGG - chr3_2:75115659-75115678 None:intergenic 35.0%
! AAAATGGTCTTCTTTTCCCA+TGG + chr3_2:75115853-75115872 Msa0424840:CDS 35.0%
CTATCTCACCATTTCTTTGA+TGG + chr3_2:75115970-75115989 Msa0424840:intron 35.0%
ACACGGAACTATCACATAAA+GGG - chr3_2:75116324-75116343 None:intergenic 35.0%
AACACGGAACTATCACATAA+AGG - chr3_2:75116325-75116344 None:intergenic 35.0%
TGTGAAGTGTGAAATCTTAG+TGG + chr3_2:75116381-75116400 Msa0424840:intron 35.0%
GTGAAGTGTGAAATCTTAGT+GGG + chr3_2:75116382-75116401 Msa0424840:intron 35.0%
TGAAGTGTGAAATCTTAGTG+GGG + chr3_2:75116383-75116402 Msa0424840:intron 35.0%
!!! TTTTTTTTGTGCTGCACTGA+GGG + chr3_2:75116414-75116433 Msa0424840:intron 35.0%
CTTTGACCAAAATTAGAAGG+TGG - chr3_2:75116674-75116693 None:intergenic 35.0%
!! CACCTTCTAATTTTGGTCAA+AGG + chr3_2:75116672-75116691 Msa0424840:intron 35.0%
ATGTGTAAATTCTCAGGTCT+AGG - chr3_2:75117003-75117022 None:intergenic 35.0%
GTTGAGAAACAGTCTCTATA+TGG + chr3_2:75117166-75117185 Msa0424840:intron 35.0%
ACATTGCAGAAAACTGAACA+AGG - chr3_2:75117290-75117309 None:intergenic 35.0%
TTCTGCTGCAAAATCATACT+CGG - chr3_2:75117313-75117332 None:intergenic 35.0%
AAGGATCTATTTGAAAAGGC+GGG + chr3_2:75117501-75117520 Msa0424840:CDS 35.0%
!! ATTTCTAGAATCTCAGAGAG+CGG - chr3_2:75117537-75117556 None:intergenic 35.0%
!! CTCTGAGATTCTAGAAATCA+AGG + chr3_2:75117539-75117558 Msa0424840:CDS 35.0%
TTCTCGTCAATGTTCAGTAA+TGG - chr3_2:75117567-75117586 None:intergenic 35.0%
AATAATGGCATATCAGGAAG+TGG - chr3_2:75117609-75117628 None:intergenic 35.0%
TTCGGTAATAATGGCATATC+AGG - chr3_2:75117615-75117634 None:intergenic 35.0%
! GAAGTTTAAGTTCGATGGAT+TGG + chr3_2:75117649-75117668 Msa0424840:CDS 35.0%
AATCTATTCCTGAGATACCA+AGG - chr3_2:75117707-75117726 None:intergenic 35.0%
!! CTCAGGAATAGATTTTGATG+CGG + chr3_2:75117713-75117732 Msa0424840:CDS 35.0%
ATGCGAAGATCATTTAGTGA+AGG + chr3_2:75117744-75117763 Msa0424840:CDS 35.0%
! GATTTCTGGATGTGAAAAGA+TGG - chr3_2:75117797-75117816 None:intergenic 35.0%
!!! TCTACTTTTAAGGTGTTCCT+AGG + chr3_2:75117883-75117902 Msa0424840:intron 35.0%
ATTTATTCCTCTCATTCGGA+TGG + chr3_2:75117907-75117926 Msa0424840:intron 35.0%
CTGCAGTTACTGGTAAAAAA+CGG - chr3_2:75117999-75118018 None:intergenic 35.0%
! TGTATCTAGAGAGCTTTTGT+AGG - chr3_2:75118034-75118053 None:intergenic 35.0%
TACAAAAGCTCTCTAGATAC+AGG + chr3_2:75118033-75118052 Msa0424840:CDS 35.0%
! AGACAAAGAGGAATTTTGGA+AGG + chr3_2:75118063-75118082 Msa0424840:CDS 35.0%
TCACATGAATAAAAAGCGAG+GGG + chr3_2:75118138-75118157 Msa0424840:intron 35.0%
!!! TTACCCTGTTTTTACACAAG+AGG - chr3_2:75118215-75118234 None:intergenic 35.0%
TCTTGTGTAAAAACAGGGTA+AGG + chr3_2:75118214-75118233 Msa0424840:intron 35.0%
GTTTAGGTCATGTTATAAGC+AGG - chr3_2:75118366-75118385 None:intergenic 35.0%
GTAGCTTCAAAAGTAGGTAA+TGG - chr3_2:75118388-75118407 None:intergenic 35.0%
TCGTTAGTAGCTTCAAAAGT+AGG - chr3_2:75118394-75118413 None:intergenic 35.0%
CATTCTTTGCAAATCTTCCA+CGG - chr3_2:75118504-75118523 None:intergenic 35.0%
TGTTATCACGTTTAACCAAG+TGG + chr3_2:75118557-75118576 Msa0424840:three_prime_UTR 35.0%
ACGTAATATGTGCTTGGAAT+TGG + chr3_2:75118620-75118639 Msa0424840:three_prime_UTR 35.0%
TGTATTAAGGTCTCTGTAGA+CGG + chr3_2:75118755-75118774 Msa0424840:three_prime_UTR 35.0%
! TTTACACATCTAGAGCTAGA+AGG - chr3_2:75118801-75118820 None:intergenic 35.0%
!! CTAGCTCTAGATGTGTAAAT+AGG + chr3_2:75118802-75118821 Msa0424840:three_prime_UTR 35.0%
TATACATGAACATAGGATGG+GGG - chr3_2:75118860-75118879 None:intergenic 35.0%
! GGATGTTACTTAGAGGAGTT+TGG - chr3_2:75115652-75115671 None:intergenic 40.0%
!! CCATTGAAGCATTGAACTTC+TGG - chr3_2:75115935-75115954 None:intergenic 40.0%
CCAGAAGTTCAATGCTTCAA+TGG + chr3_2:75115932-75115951 Msa0424840:CDS 40.0%
TCTCATAGTAGTAGCACAAG+TGG + chr3_2:75116217-75116236 Msa0424840:intron 40.0%
!!! CTTTTTTTTGTGCTGCACTG+AGG + chr3_2:75116413-75116432 Msa0424840:intron 40.0%
!!! TTTTTTTGTGCTGCACTGAG+GGG + chr3_2:75116415-75116434 Msa0424840:intron 40.0%
!! CGCTTGCCTCCTTTAAAATA+AGG + chr3_2:75116465-75116484 Msa0424840:intron 40.0%
!!! CAAATGATGGTTTTGAGGCT+AGG + chr3_2:75116623-75116642 Msa0424840:intron 40.0%
CTTGTAAATCCCTACACTCA+AGG - chr3_2:75116709-75116728 None:intergenic 40.0%
CTTGAGTGTAGGGATTTACA+AGG + chr3_2:75116707-75116726 Msa0424840:intron 40.0%
AGTGTAGGGATTTACAAGGA+TGG + chr3_2:75116711-75116730 Msa0424840:intron 40.0%
AGGGATTTACAAGGATGGTT+AGG + chr3_2:75116716-75116735 Msa0424840:intron 40.0%
! GAGTATGATTTTGCAGCAGA+AGG + chr3_2:75117312-75117331 Msa0424840:CDS 40.0%
ATGCCTGACTCTTCAATCAT+CGG - chr3_2:75117360-75117379 None:intergenic 40.0%
GCCATCTCAATGATATCTTG+TGG + chr3_2:75117402-75117421 Msa0424840:CDS 40.0%
GCCACAAGATATCATTGAGA+TGG - chr3_2:75117406-75117425 None:intergenic 40.0%
TCTATTTGAAAAGGCGGGAA+TGG + chr3_2:75117506-75117525 Msa0424840:CDS 40.0%
CTAACGCTCTTCGGTAATAA+TGG - chr3_2:75117624-75117643 None:intergenic 40.0%
! CTCAGGAAGTTTAAGTTCGA+TGG + chr3_2:75117644-75117663 Msa0424840:CDS 40.0%
CACATCCAGAAATCGGAAAT+CGG + chr3_2:75117803-75117822 Msa0424840:intron 40.0%
AGTATCCGATTTCCGATTTC+TGG - chr3_2:75117811-75117830 None:intergenic 40.0%
!!! CTGTTTTTGTGACAGACTCT+TGG + chr3_2:75117933-75117952 Msa0424840:intron 40.0%
ACAGGAACAAGAAGACAAAG+AGG + chr3_2:75118051-75118070 Msa0424840:CDS 40.0%
ACAGCCTCTTGTGTAAAAAC+AGG + chr3_2:75118208-75118227 Msa0424840:intron 40.0%
CAGCCTCTTGTGTAAAAACA+GGG + chr3_2:75118209-75118228 Msa0424840:intron 40.0%
CGTACAATACACCAAATGGT+GGG + chr3_2:75118240-75118259 Msa0424840:intron 40.0%
AGAATCTGATCAGGCAAAGA+AGG + chr3_2:75118525-75118544 Msa0424840:CDS 40.0%
CTTTGTCACATAAAGCCACT+TGG - chr3_2:75118575-75118594 None:intergenic 40.0%
TGCTCAACGTAATATGTGCT+TGG + chr3_2:75118614-75118633 Msa0424840:three_prime_UTR 40.0%
CAAACTTGAATTGCTTGGAC+TGG + chr3_2:75118983-75119002 Msa0424840:three_prime_UTR 40.0%
!!! TGGTTTTGAGGCTAGGTCTT+TGG + chr3_2:75116630-75116649 Msa0424840:intron 45.0%
! AGGCTAGGTCTTTGGTTTAG+TGG + chr3_2:75116638-75116657 Msa0424840:intron 45.0%
GTAAATCCCTACACTCAAGG+TGG - chr3_2:75116706-75116725 None:intergenic 45.0%
!! GACTCTTCAATCATCGGTTC+AGG - chr3_2:75117354-75117373 None:intergenic 45.0%
GAACCGATGATTGAAGAGTC+AGG + chr3_2:75117354-75117373 Msa0424840:CDS 45.0%
ATTGAGATGGCTGCTGTCAT+GGG - chr3_2:75117393-75117412 None:intergenic 45.0%
!! AGTTCGATGGATTGGATGCA+TGG + chr3_2:75117657-75117676 Msa0424840:CDS 45.0%
ACTGTTTGTGACTCCACACA+AGG - chr3_2:75117862-75117881 None:intergenic 45.0%
ACAGAAACCATCCGAATGAG+AGG - chr3_2:75117917-75117936 None:intergenic 45.0%
TAAAAAGCGAGGGGTAACCT+TGG + chr3_2:75118147-75118166 Msa0424840:intron 45.0%
! TTACACAAGAGGCTGTTTCC+AGG - chr3_2:75118204-75118223 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG + chr3_2:75118236-75118255 Msa0424840:intron 45.0%
GCGTACAATACACCAAATGG+TGG + chr3_2:75118239-75118258 Msa0424840:intron 45.0%
CATGTGAAGAAAGGGCAATC+CGG - chr3_2:75118306-75118325 None:intergenic 45.0%
! GTTGGTTTCAGTATGCTTGC+AGG + chr3_2:75118449-75118468 Msa0424840:intron 45.0%
GTTTCAGTATGCTTGCAGGA+AGG + chr3_2:75118453-75118472 Msa0424840:intron 45.0%
GTAGCACAAGTGGAAACAGC+TGG + chr3_2:75116227-75116246 Msa0424840:intron 50.0%
GCACTGAGGGGAGATTAAAG+AGG + chr3_2:75116427-75116446 Msa0424840:intron 50.0%
! AGAGTGTCCACCTTGAGTGT+AGG + chr3_2:75116696-75116715 Msa0424840:intron 50.0%
GAGTGTCCACCTTGAGTGTA+GGG + chr3_2:75116697-75116716 Msa0424840:intron 50.0%
ATCTGCGATAGTGAAGCGAC+AGG - chr3_2:75117090-75117109 None:intergenic 50.0%
CATTGAGATGGCTGCTGTCA+TGG - chr3_2:75117394-75117413 None:intergenic 50.0%
!! TTACCGAAGAGCGTTAGCTC+AGG + chr3_2:75117627-75117646 Msa0424840:CDS 50.0%
CTTCCTGAGCTAACGCTCTT+CGG - chr3_2:75117633-75117652 None:intergenic 50.0%
GCAATGAAGCCTGCTTTCCT+TGG + chr3_2:75117687-75117706 Msa0424840:CDS 50.0%
CCTGAGATACCAAGGAAAGC+AGG - chr3_2:75117699-75117718 None:intergenic 50.0%
CCTGCTTTCCTTGGTATCTC+AGG + chr3_2:75117696-75117715 Msa0424840:CDS 50.0%
GTAAAAAACGGCCTCTCCAC+GGG - chr3_2:75117987-75118006 None:intergenic 50.0%
GGTAAAAAACGGCCTCTCCA+CGG - chr3_2:75117988-75118007 None:intergenic 50.0%
TACCAGTAACTGCAGCAGTG+AGG + chr3_2:75118004-75118023 Msa0424840:CDS 50.0%
CGCAACTGACTGTAAGGTCA+CGG + chr3_2:75118170-75118189 Msa0424840:intron 50.0%
GCAACTGACTGTAAGGTCAC+GGG + chr3_2:75118171-75118190 Msa0424840:intron 50.0%
GCAAATCTTCCACGGACTCT+TGG - chr3_2:75118496-75118515 None:intergenic 50.0%
TACAACTCCTCCACCAAGTG+TGG - chr3_2:75118665-75118684 None:intergenic 50.0%
TGGCTGCTGTCATGGGATCA+AGG - chr3_2:75117386-75117405 None:intergenic 55.0%
GAACGTTGTCCAATCTCCCG+TGG + chr3_2:75117968-75117987 Msa0424840:CDS 55.0%
TTGTCCAATCTCCCGTGGAG+AGG + chr3_2:75117973-75117992 Msa0424840:CDS 55.0%
CTCCTCACTGCTGCAGTTAC+TGG - chr3_2:75118009-75118028 None:intergenic 55.0%
CCTTACAGTCAGTTGCGCCA+AGG - chr3_2:75118167-75118186 None:intergenic 55.0%
CCTTGGCGCAACTGACTGTA+AGG + chr3_2:75118164-75118183 Msa0424840:intron 55.0%
AAGGTCACGGGTTCAAGTCC+TGG + chr3_2:75118183-75118202 Msa0424840:intron 55.0%
GACAGTCAACCAAGAGTCCG+TGG + chr3_2:75118484-75118503 Msa0424840:CDS 55.0%
CTCCTGTGTGTGTCCACACT+TGG + chr3_2:75118649-75118668 Msa0424840:three_prime_UTR 55.0%
CACCAAGTGTGGACACACAC+AGG - chr3_2:75118654-75118673 None:intergenic 55.0%
CTGTGTGTGTCCACACTTGG+TGG + chr3_2:75118652-75118671 Msa0424840:three_prime_UTR 55.0%
! TGTGTGTCCACACTTGGTGG+AGG + chr3_2:75118655-75118674 Msa0424840:three_prime_UTR 55.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr3_2:75118252-75118271 Msa0424840:intron 60.0%
CTGAAGCTCCCGCATACGCA+GGG - chr3_2:75118279-75118298 None:intergenic 60.0%
ACTGAAGCTCCCGCATACGC+AGG - chr3_2:75118280-75118299 None:intergenic 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG + chr3_2:75118284-75118303 Msa0424840:intron 60.0%
ACGGCCTCTCCACGGGAGAT+TGG - chr3_2:75117980-75117999 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG - chr3_2:75118254-75118273 None:intergenic 65.0%
CATACGCAGGGTCCGGGAAG+GGG - chr3_2:75118267-75118286 None:intergenic 65.0%
GCATACGCAGGGTCCGGGAA+GGG - chr3_2:75118268-75118287 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG + chr3_2:75118267-75118286 Msa0424840:intron 65.0%
TTCCCGGACCCTGCGTATGC+GGG + chr3_2:75118268-75118287 Msa0424840:intron 65.0%
CGCATACGCAGGGTCCGGGA+AGG - chr3_2:75118269-75118288 None:intergenic 70.0%
CTCCCGCATACGCAGGGTCC+GGG - chr3_2:75118273-75118292 None:intergenic 70.0%
GCTCCCGCATACGCAGGGTC+CGG - chr3_2:75118274-75118293 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 75115645 75119034 75115645 ID=Msa0424840;Name=Msa0424840
chr3_2 mRNA 75115645 75119034 75115645 ID=Msa0424840-mRNA-1;Parent=Msa0424840;Name=Msa0424840-mRNA-1;_AED=0.12;_eAED=0.12;_QI=198|1|1|1|1|1|4|482|281
chr3_2 exon 75115645 75115953 75115645 ID=Msa0424840-mRNA-1:exon:19145;Parent=Msa0424840-mRNA-1
chr3_2 exon 75117282 75117776 75117282 ID=Msa0424840-mRNA-1:exon:19146;Parent=Msa0424840-mRNA-1
chr3_2 exon 75117948 75118094 75117948 ID=Msa0424840-mRNA-1:exon:19147;Parent=Msa0424840-mRNA-1
chr3_2 exon 75118460 75119034 75118460 ID=Msa0424840-mRNA-1:exon:19148;Parent=Msa0424840-mRNA-1
chr3_2 five_prime_UTR 75115645 75115842 75115645 ID=Msa0424840-mRNA-1:five_prime_utr;Parent=Msa0424840-mRNA-1
chr3_2 CDS 75115843 75115953 75115843 ID=Msa0424840-mRNA-1:cds;Parent=Msa0424840-mRNA-1
chr3_2 CDS 75117282 75117776 75117282 ID=Msa0424840-mRNA-1:cds;Parent=Msa0424840-mRNA-1
chr3_2 CDS 75117948 75118094 75117948 ID=Msa0424840-mRNA-1:cds;Parent=Msa0424840-mRNA-1
chr3_2 CDS 75118460 75118552 75118460 ID=Msa0424840-mRNA-1:cds;Parent=Msa0424840-mRNA-1
chr3_2 three_prime_UTR 75118553 75119034 75118553 ID=Msa0424840-mRNA-1:three_prime_utr;Parent=Msa0424840-mRNA-1
Gene Sequence

>Msa0424840

ATGTATAGAGAAAATGGTCTTCTTTTCCCATGGTCTTATCTACACAATTTCTCTCAAGAGCTTCATCAACTTGAAGACTACTACAGAAACCAGAAGTTCAATGCTTCAATGAGTGACCTTGTTCAGTTTTCTGCAATGTCCGAGTATGATTTTGCAGCAGAAGGAGATCTGTTCAAAGCACCTGAACCGATGATTGAAGAGTCAGGCATTGACCTTGATCCCATGACAGCAGCCATCTCAATGATATCTTGTGGCGAAGATGTCAAGTCCACAGATATTTCTATTCTTCAAAACGAACAGCTTCTCAGCGATGTATTCTATGATTGCAAAAAGGATCTATTTGAAAAGGCGGGAATGGAGTCACCGCTCTCTGAGATTCTAGAAATCAAGGTTCCATTACTGAACATTGACGAGAACATAATTCAAGAAAACAAGCCACTTCCTGATATGCCATTATTACCGAAGAGCGTTAGCTCAGGAAGTTTAAGTTCGATGGATTGGATGCATGGATCTGCTGCAATGAAGCCTGCTTTCCTTGGTATCTCAGGAATAGATTTTGATGCGGTTTATGGCATGCGAAGATCATTTAGTGAAGGAGATATAAAGACTCTTGGCAATGCTAATATGAACGTTGTCCAATCTCCCGTGGAGAGGCCGTTTTTTACCAGTAACTGCAGCAGTGAGGAGCGCCTACAAAAGCTCTCTAGATACAGGAACAAGAAGACAAAGAGGAATTTTGGAAGGAAAATCAAGTATGCTTGCAGGAAGGCTCTAGCTGACAGTCAACCAAGAGTCCGTGGAAGATTTGCAAAGAATGAAGAATCTGATCAGGCAAAGAAGGACTGA
Protein sequence

>Msa0424840

MYRENGLLFPWSYLHNFSQELHQLEDYYRNQKFNASMSDLVQFSAMSEYDFAAEGDLFKAPEPMIEESGIDLDPMTAAISMISCGEDVKSTDISILQNEQLLSDVFYDCKKDLFEKAGMESPLSEILEIKVPLLNIDENIIQENKPLPDMPLLPKSVSSGSLSSMDWMHGSAAMKPAFLGISGIDFDAVYGMRRSFSEGDIKTLGNANMNVVQSPVERPFFTSNCSSEERLQKLSRYRNKKTKRNFGRKIKYACRKALADSQPRVRGRFAKNEESDQAKKD*