AlfalfaGEDB | Msa0427270

M. sativa cultivar zhongmu-4 / Msa0427270

KEGG: K08269
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008589 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010646 GO:0010647 GO:0010648 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023051 GO:0023056 GO:0023057 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045879 GO:0045880 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050789 GO:0050794 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427270	XP_003602555.1	79.939	329	16	2	2	322	4	290	0.0	521

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427270	sp\|F4I1N8\|ATG1T_ARATH	48.485	297	103	2	23	311	7	261	1.21e-91	281

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427270	G7JAY4	79.939	329	16	2	2	322	4	290	0.0	521

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0427270	Msa0517780	0.837651	4.584097e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427270	MtrunA17_Chr3g0129721	79.939	329	16	2	2	322	4	290	0.0	521
Msa0427270	MtrunA17_Chr4g0008561	43.189	301	120	3	23	315	19	276	1.59e-76	248
Msa0427270	MtrunA17_Chr8g0345801	39.024	287	125	2	23	301	15	259	2.31e-73	227
Msa0427270	MtrunA17_Chr3g0129701	90.411	73	7	0	223	295	14	86	1.44e-44	147
Msa0427270	MtrunA17_Chr5g0441011	32.990	291	150	5	23	309	13	262	2.46e-37	138
Msa0427270	MtrunA17_Chr5g0432491	32.432	296	159	5	23	316	23	279	5.28e-36	134
Msa0427270	MtrunA17_Chr2g0284011	29.866	298	163	7	23	316	12	267	1.93e-34	130
Msa0427270	MtrunA17_Chr1g0203881	28.289	304	164	6	23	316	41	300	3.31e-34	130
Msa0427270	MtrunA17_Chr2g0304111	29.508	305	170	5	16	316	16	279	7.05e-34	129
Msa0427270	MtrunA17_Chr5g0432471	29.412	306	164	6	23	321	12	272	1.01e-33	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427270	AT1G49180.2	48.485	297	103	2	23	311	7	261	3.67e-93	282
Msa0427270	AT1G49180.1	48.485	297	103	2	23	311	7	261	1.23e-92	281
Msa0427270	AT2G37840.1	42.434	304	124	3	23	318	12	272	5.50e-77	250
Msa0427270	AT3G53930.1	41.776	304	126	3	23	318	20	280	3.05e-74	242
Msa0427270	AT3G53930.2	41.776	304	126	3	23	318	20	280	3.07e-74	242
Msa0427270	AT3G61960.2	40.397	302	130	2	23	316	10	269	1.91e-73	237
Msa0427270	AT3G61960.1	40.397	302	130	2	23	316	10	269	2.63e-73	238
Msa0427270	AT1G30270.2	30.476	315	152	7	23	322	31	293	1.87e-39	145
Msa0427270	AT1G12580.1	30.693	303	156	6	23	315	44	302	1.93e-39	145
Msa0427270	AT1G30270.1	30.476	315	152	7	23	322	31	293	2.10e-39	145

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Find 79 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACCTTCTCCAATTT+TGG	0.070296	3_2:-77957860	None:intergenic
AAAATTGATCTCACAGTCCA+AGG	0.556152	3_2:-77957977	None:intergenic
AAACCAGGGGATTGCTGTCA+AGG	0.499609	3_2:-77959828	None:intergenic
AAAGCAGAACAAAGACCAAG+TGG	0.701880	3_2:+77957895	Msa0427270:CDS
AACATTAGTTAAAATAACAT+CGG	0.621971	3_2:+77957698	None:intergenic
AAGTTGCTTCATGAATCTTC+TGG	0.468798	3_2:-77958230	None:intergenic
AATAAAATTCATCAAAAGAG+AGG	0.573975	3_2:-77959773	None:intergenic
AATATACATAAAGTACATGA+AGG	0.591653	3_2:-77960107	None:intergenic
ACACAGAGACTTCTCAAAAG+TGG	0.501019	3_2:-77959977	None:intergenic
ACATTATTTCTGCCGTTAAA+AGG	0.253516	3_2:-77959438	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTCTGTCTTTGTAAAAATGT+TGG	+	chr3_2:77957733-77957752	Msa0427270:five_prime_UTR	30.0%
TTAAAGTCCAAAATTGGAGA+AGG	+	chr3_2:77957853-77957872	Msa0427270:CDS	30.0%
TGAAGAAGGTGAACAATATT+AGG	-	chr3_2:77958021-77958040	None:intergenic	30.0%
TTTCTGTATATACCAAGTTG+TGG	+	chr3_2:77959093-77959112	Msa0427270:intron	30.0%
ATTTAGAAGCTCAAAAAGCA+TGG	-	chr3_2:77959409-77959428	None:intergenic	30.0%
TTAATATCAGCAGTAATCCT+AGG	-	chr3_2:77959509-77959528	None:intergenic	30.0%
TCTATTCAATCAACTACTTG+TGG	+	chr3_2:77959692-77959711	Msa0427270:intron	30.0%
GCTAAGGAAATAATAAAACC+AGG	-	chr3_2:77959846-77959865	None:intergenic	30.0%
ATGAAGTACATAGGATCAAT+AGG	-	chr3_2:77960141-77960160	None:intergenic	30.0%
AAAATTGATCTCACAGTCCA+AGG	-	chr3_2:77957980-77957999	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	77957712	77960210	77957712	ID=Msa0427270;Name=Msa0427270
chr3_2	mRNA	77957712	77960210	77957712	ID=Msa0427270-mRNA-1;Parent=Msa0427270;Name=Msa0427270-mRNA-1;_AED=0.24;_eAED=0.24;_QI=69\|0.71\|0.87\|1\|1\|1\|8\|377\|322
chr3_2	exon	77957712	77958050	77957712	ID=Msa0427270-mRNA-1:exon:20395;Parent=Msa0427270-mRNA-1
chr3_2	exon	77958136	77958253	77958136	ID=Msa0427270-mRNA-1:exon:20396;Parent=Msa0427270-mRNA-1
chr3_2	exon	77958392	77958450	77958392	ID=Msa0427270-mRNA-1:exon:20397;Parent=Msa0427270-mRNA-1
chr3_2	exon	77958611	77958791	77958611	ID=Msa0427270-mRNA-1:exon:20398;Parent=Msa0427270-mRNA-1
chr3_2	exon	77958929	77959008	77958929	ID=Msa0427270-mRNA-1:exon:20399;Parent=Msa0427270-mRNA-1
chr3_2	exon	77959384	77959464	77959384	ID=Msa0427270-mRNA-1:exon:20400;Parent=Msa0427270-mRNA-1
chr3_2	exon	77959544	77959655	77959544	ID=Msa0427270-mRNA-1:exon:20401;Parent=Msa0427270-mRNA-1
chr3_2	exon	77959766	77960210	77959766	ID=Msa0427270-mRNA-1:exon:20402;Parent=Msa0427270-mRNA-1
chr3_2	five_prime_UTR	77957712	77957780	77957712	ID=Msa0427270-mRNA-1:five_prime_utr;Parent=Msa0427270-mRNA-1
chr3_2	CDS	77957781	77958050	77957781	ID=Msa0427270-mRNA-1:cds;Parent=Msa0427270-mRNA-1
chr3_2	CDS	77958136	77958253	77958136	ID=Msa0427270-mRNA-1:cds;Parent=Msa0427270-mRNA-1
chr3_2	CDS	77958392	77958450	77958392	ID=Msa0427270-mRNA-1:cds;Parent=Msa0427270-mRNA-1
chr3_2	CDS	77958611	77958791	77958611	ID=Msa0427270-mRNA-1:cds;Parent=Msa0427270-mRNA-1
chr3_2	CDS	77958929	77959008	77958929	ID=Msa0427270-mRNA-1:cds;Parent=Msa0427270-mRNA-1
chr3_2	CDS	77959384	77959464	77959384	ID=Msa0427270-mRNA-1:cds;Parent=Msa0427270-mRNA-1
chr3_2	CDS	77959544	77959655	77959544	ID=Msa0427270-mRNA-1:cds;Parent=Msa0427270-mRNA-1
chr3_2	CDS	77959766	77959833	77959766	ID=Msa0427270-mRNA-1:cds;Parent=Msa0427270-mRNA-1
chr3_2	three_prime_UTR	77959834	77960210	77959834	ID=Msa0427270-mRNA-1:three_prime_utr;Parent=Msa0427270-mRNA-1

Gene Sequence

>Msa0427270

ATGGAATGTGATCGTGATTTTGAAGAAAAGTTGGTAAGAGAAGAAGGAAACAAAAAAAACTACTACTATGTTTTAAAGTCCAAAATTGGAGAAGGTTCTTTTTCCACAGTGTGGAAAGCAGAACAAAGACCAAGTGGTGAAGATGTTGCGGTGAAGCAAGTCTTTCTCTCTAAACTCAATCCTCGCCTCAAAGCTTCCTTGGACTGTGAGATCAATTTTATGTCTTCTGTTAATCATCCTAATATTGTTCACCTTCTTCACTTCTTCCAGGGTAATGGATGTGTTTACTTGGTCCTTGAGTTTTGTGCTGGAGGGAATCTAGCTTCTTATATTCGATGCCATGGAAGAGTTCATCAACTAACCGCCAGAAGATTCATGAAGCAACTTGGCTCTGGTTTAAAAGTACTACACTCTCATGGCATCATTCATAGAGACTTGAAACCCGAGTCACGGGGTTGTGACAGCACTATACTTTTGTCTTTTAACAGTGAAAAACATGAGCTCTTTATGTTACTGATAGTGAATTATTTACCTGAAACTTGTCTGAATTGGCTGCTATTGTTGCGGAATCAGAACATTTTGCTATCAAGCCATGGTGCCGATGCAGTGCTGAAGATAGCTGATTTTGGTGAGTATGTTGAGACAGTTTGTGGAACTCCATTGTACATGGCTCCTGAAGTTCTTCAGTTCCAGAGATATGATCACAAGGCAGACATGTGGAGTGTTGGGGCCATGCTTTTTGAGCTTCTAAATGGTTATCCACCTTTTAACGGCAGAAATAATGTTCAGGTGTTGAAAAACATCAGATCATGTACCTGTCTTCCATTTTCTCAATCGATTCTTTATGGATTGGATTCTGCCTGTCTTGATATTTGTAAAAGACTACTGTGCTTAAATCCAGTTGAGCGCCTCTCTTTTGATGAATTTTATTTCCATAGCTTTCTGCGAGGAAAATCACTGGGACCTTGA

Protein sequence

>Msa0427270

MECDRDFEEKLVREEGNKKNYYYVLKSKIGEGSFSTVWKAEQRPSGEDVAVKQVFLSKLNPRLKASLDCEINFMSSVNHPNIVHLLHFFQGNGCVYLVLEFCAGGNLASYIRCHGRVHQLTARRFMKQLGSGLKVLHSHGIIHRDLKPESRGCDSTILLSFNSEKHELFMLLIVNYLPETCLNWLLLLRNQNILLSSHGADAVLKIADFGEYVETVCGTPLYMAPEVLQFQRYDHKADMWSVGAMLFELLNGYPPFNGRNNVQVLKNIRSCTCLPFSQSILYGLDSACLDICKRLLCLNPVERLSFDEFYFHSFLRGKSLGP*