AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0436390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 74 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATGAGTATTTATTATT+AGG 0.177326 3_2:+89954088 Msa0436390:CDS
ACAAGTAGGCCAAGCTTTAA+AGG 0.207093 3_2:+89954055 Msa0436390:CDS
TTCTCCTGCTGGGGCTGTTT+TGG 0.216056 3_2:-89954574 None:intergenic
GATGAAGTGGCTAACTTCTT+TGG 0.243853 3_2:+89954750 Msa0436390:CDS
TCTTCCTTCAAAGGCGAATT+AGG 0.249175 3_2:-89955252 None:intergenic
TGATCTGGATGGGCTGGTTT+TGG 0.253155 3_2:-89954677 None:intergenic
TTGTGTCTTAAAACTCAAAT+TGG 0.272781 3_2:-89953984 None:intergenic
GAGTGTTGGAACAGCGGTTC+TGG 0.285086 3_2:-89952548 None:intergenic
GGTATTCAACACCATCTATC+AGG 0.287546 3_2:+89953855 Msa0436390:CDS
TTTGGTGCTGTTAGTTTGCT+TGG 0.337190 3_2:-89954556 None:intergenic
TTGTGTCTTCGTGGGTGATC+TGG 0.345533 3_2:-89954692 None:intergenic
GAGAGAGAGTGAGAAGGTTC+TGG 0.363477 3_2:-89952477 None:intergenic
TAGGCTAAGAAATGCTATAA+TGG 0.368746 3_2:-89951320 None:intergenic
TTGATCATCCAAATGCATAT+TGG 0.373587 3_2:+89954020 Msa0436390:CDS
GCTGCAACATCAGCGCATAT+TGG 0.384834 3_2:+89951403 Msa0436390:CDS
CATGTGGTGGAAGCAACTTC+TGG 0.387853 3_2:+89955279 Msa0436390:CDS
TGAGCCTAATTCGCCTTTGA+AGG 0.392394 3_2:+89955248 Msa0436390:CDS
CTGCAACATCAGCGCATATT+GGG 0.393585 3_2:+89951404 Msa0436390:CDS
CAAGTAGGCCAAGCTTTAAA+GGG 0.398181 3_2:+89954056 Msa0436390:CDS
TAACCAGTCCAATATGCATT+TGG 0.411552 3_2:-89954028 None:intergenic
TAATTAACTCCCCTGATAGA+TGG 0.425696 3_2:-89953866 None:intergenic
AAGAAGTTAGCCACTTCATC+AGG 0.436830 3_2:-89954747 None:intergenic
CGTGGGTGATCTGGATGGGC+TGG 0.438475 3_2:-89954683 None:intergenic
TCTCGCAGCCCTTTAAAGCT+TGG 0.447124 3_2:-89954064 None:intergenic
TCTGCTGCAGCTGATCGGTT+TGG 0.461175 3_2:-89954515 None:intergenic
CATCACTGTCTCCCTCATAA+AGG 0.470138 3_2:+89952650 Msa0436390:CDS
GAAGGGGAAGGGACATGAAT+GGG 0.476028 3_2:-89953928 None:intergenic
CGAAGGGGAAGGGACATGAA+TGG 0.483429 3_2:-89953929 None:intergenic
GTCTTCGTGGGTGATCTGGA+TGG 0.483633 3_2:-89954688 None:intergenic
TTGGTGCTGTTAGTTTGCTT+GGG 0.489173 3_2:-89954555 None:intergenic
GTGCTTCTGCTGCAGCTGAT+CGG 0.497136 3_2:-89954520 None:intergenic
GAGGGAGAGAGTGAGAGTGA+TGG 0.504909 3_2:-89952610 None:intergenic
TAGAGGAAGAGAGTGAGTGT+TGG 0.525207 3_2:-89952562 None:intergenic
TTTACTTGTTGTGTCTTCGT+GGG 0.529294 3_2:-89954700 None:intergenic
CGGTTGTATATCAGAAAAGC+TGG 0.532039 3_2:-89954610 None:intergenic
AGAGAGTGAGAGTGATGGAG+TGG 0.535010 3_2:-89952605 None:intergenic
GTGTGGCAACTTTGAAGCGA+AGG 0.535780 3_2:-89953946 None:intergenic
AACTTTGAAGCGAAGGGGAA+GGG 0.539357 3_2:-89953939 None:intergenic
TCTTCGTGGGTGATCTGGAT+GGG 0.539760 3_2:-89954687 None:intergenic
CATCCAAATGCATATTGGAC+TGG 0.541163 3_2:+89954025 Msa0436390:CDS
TAATTCGCCTTTGAAGGAAG+AGG 0.547350 3_2:+89955254 Msa0436390:CDS
AGCACCAAAACAGCCCCAGC+AGG 0.549550 3_2:+89954570 Msa0436390:CDS
ACATGATCCTCTTCCTTCAA+AGG 0.555993 3_2:-89955261 None:intergenic
CAACTTTGAAGCGAAGGGGA+AGG 0.560038 3_2:-89953940 None:intergenic
GCGGCGAGAGTGAGAAAGAG+AGG 0.563004 3_2:-89952516 None:intergenic
ATATAGGATTCCTGATGAAG+TGG 0.570036 3_2:+89954737 Msa0436390:CDS
GTATTCAACACCATCTATCA+GGG 0.570537 3_2:+89953856 Msa0436390:CDS
GCTGTTCTGCTATCTGCCAT+CGG 0.572966 3_2:-89954630 None:intergenic
TAAGAAATGCTATAATGGAA+AGG 0.583042 3_2:-89951315 None:intergenic
ATGGCAGATAGCAGAACAGC+AGG 0.586850 3_2:+89954633 Msa0436390:CDS
CTTTATGAGGGAGACAGTGA+TGG 0.595538 3_2:-89952649 None:intergenic
TGTGGCAACTTTGAAGCGAA+GGG 0.596360 3_2:-89953945 None:intergenic
TCTTCCTCTATGACTCTCGC+CGG 0.620604 3_2:+89952575 Msa0436390:CDS
TCCACTGTGAAAACAAGCAT+AGG 0.626022 3_2:-89951339 None:intergenic
GTGATGGAGTGGTTTGACAC+CGG 0.627014 3_2:-89952594 None:intergenic
GACACCGGCGAGAGTCATAG+AGG 0.634984 3_2:-89952579 None:intergenic
GTGAGTGAGAGAGAGTGAGA+AGG 0.635071 3_2:-89952483 None:intergenic
GAGAGTGAGTGTTGGAACAG+CGG 0.636227 3_2:-89952554 None:intergenic
GGAACAATGAAAGTGACGGA+GGG 0.640256 3_2:-89952628 None:intergenic
ACAGCGGTTCTGGAGAGTCA+CGG 0.645545 3_2:-89952538 None:intergenic
GTGGTGGAAGCAACTTCTGG+TGG 0.652187 3_2:+89955282 Msa0436390:CDS
TGATGGAACAATGAAAGTGA+CGG 0.652546 3_2:-89952632 None:intergenic
TGGAACAATGAAAGTGACGG+AGG 0.672484 3_2:-89952629 None:intergenic
GTGGAGGCAAGTTGGACGTG+CGG 0.674547 3_2:+89951357 Msa0436390:CDS
TTTGAAGGAAGAGGATCATG+TGG 0.680203 3_2:+89955263 Msa0436390:CDS
GAAGGAAGAGGATCATGTGG+TGG 0.682569 3_2:+89955266 Msa0436390:CDS
GTAGTTATGAAGCTACCTCG+TGG 0.688871 3_2:+89951379 Msa0436390:CDS
GTGGCAACTTTGAAGCGAAG+GGG 0.692328 3_2:-89953944 None:intergenic
AATGGGAAGTGAGAGAAGCG+AGG 0.700969 3_2:-89953911 None:intergenic
GGAGCTGTCTAAATTATGTG+TGG 0.714374 3_2:-89953963 None:intergenic
GCTGATGTTGCAGCTCCACG+AGG 0.725863 3_2:-89951394 None:intergenic
TATTCAACACCATCTATCAG+GGG 0.731418 3_2:+89953857 Msa0436390:CDS
TGGGAGTAACATCATCACTG+TGG 0.739104 3_2:+89951423 Msa0436390:CDS
GCGGTTCTGGAGAGTCACGG+CGG 0.785786 3_2:-89952535 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATGAAATTAATAATTCT+AGG + chr3_2:89952758-89952777 Msa0436390:intron 10.0%
!! TAATGAAATTAATAATTCTA+GGG + chr3_2:89952759-89952778 Msa0436390:intron 10.0%
!! AATAATGAGTATTTATTATT+AGG + chr3_2:89954088-89954107 Msa0436390:CDS 10.0%
!!! GTTATTTTACTTTTTAATTT+AGG + chr3_2:89954940-89954959 Msa0436390:intron 10.0%
!! TTTAAATTGACAAATACAAA+AGG + chr3_2:89951470-89951489 Msa0436390:intron 15.0%
!! TTAAATTGACAAATACAAAA+GGG + chr3_2:89951471-89951490 Msa0436390:intron 15.0%
!! AAGAAAATTATTACCAAAAA+AGG - chr3_2:89952094-89952113 None:intergenic 15.0%
!!! TCTTTTTTATATCCTTAAAA+TGG + chr3_2:89952110-89952129 Msa0436390:intron 15.0%
!!! TAATATTAGTTTCCATTTTA+AGG - chr3_2:89952125-89952144 None:intergenic 15.0%
!! GAAACTAATATTAATATTGT+TGG + chr3_2:89952132-89952151 Msa0436390:intron 15.0%
!!! AAAAAATTGCTTTAATTTTG+CGG - chr3_2:89952320-89952339 None:intergenic 15.0%
!!! TTATTGAACAAATTGATTTA+TGG - chr3_2:89952693-89952712 None:intergenic 15.0%
!!! AATCAATTTGTTCAATAATT+TGG + chr3_2:89952695-89952714 Msa0436390:intron 15.0%
!!! ATCAATTTGTTCAATAATTT+GGG + chr3_2:89952696-89952715 Msa0436390:intron 15.0%
!! ATTTCATTAAAACAATTCAA+AGG - chr3_2:89952748-89952767 None:intergenic 15.0%
!!! TACAATTTTAAGACAATTTT+AGG + chr3_2:89952845-89952864 Msa0436390:intron 15.0%
!!! ACAATTTTAAGACAATTTTA+GGG + chr3_2:89952846-89952865 Msa0436390:intron 15.0%
!!! TAATTAATTTTGTTGTCTTA+GGG + chr3_2:89952948-89952967 Msa0436390:intron 15.0%
!! ACATGAATTAAACTATTATT+AGG - chr3_2:89953652-89953671 None:intergenic 15.0%
!!! AATAGTTTAATTCATGTTTT+TGG + chr3_2:89953655-89953674 Msa0436390:intron 15.0%
!! GTTCAAAAAAAAAAAGATAT+AGG - chr3_2:89954394-89954413 None:intergenic 15.0%
!! AAAATTTGTGCATACATAAA+TGG - chr3_2:89951603-89951622 None:intergenic 20.0%
!! AAGCATAAAAATAAAAACTC+AGG - chr3_2:89951719-89951738 None:intergenic 20.0%
!!! GTAAATTCAAGTTTTCATTA+TGG + chr3_2:89951980-89951999 Msa0436390:intron 20.0%
!! ATAATGAAAACAACCAAAAA+AGG - chr3_2:89952026-89952045 None:intergenic 20.0%
!!! GAAATATTTTGAACTCTTTA+TGG - chr3_2:89952072-89952091 None:intergenic 20.0%
!!! GTTCAAAATATTTCCTTTTT+TGG + chr3_2:89952078-89952097 Msa0436390:intron 20.0%
!!! AGTATTTGTTACAACTAAAA+GGG - chr3_2:89952271-89952290 None:intergenic 20.0%
!!! AAGTATTTGTTACAACTAAA+AGG - chr3_2:89952272-89952291 None:intergenic 20.0%
!!! AAAAATTGCTTTAATTTTGC+GGG - chr3_2:89952319-89952338 None:intergenic 20.0%
!!! TTTTAATGAAATAGAAGAGA+GGG - chr3_2:89952400-89952419 None:intergenic 20.0%
!!! TTTTTAATGAAATAGAAGAG+AGG - chr3_2:89952401-89952420 None:intergenic 20.0%
!! AGAGATAATAATGTGATAAA+GGG - chr3_2:89952442-89952461 None:intergenic 20.0%
!! GAATAAAAATACCTTTATGA+GGG - chr3_2:89952664-89952683 None:intergenic 20.0%
!! AGAATAAAAATACCTTTATG+AGG - chr3_2:89952665-89952684 None:intergenic 20.0%
!! TGTAATATTGCAGAAAATAT+TGG - chr3_2:89952829-89952848 None:intergenic 20.0%
!!! GTAATTAATTTTGTTGTCTT+AGG + chr3_2:89952947-89952966 Msa0436390:intron 20.0%
!!! TACAGATTTTAGTACTTTAT+AGG + chr3_2:89953421-89953440 Msa0436390:intron 20.0%
!! GAACAATAGACATAATAAAT+TGG - chr3_2:89953504-89953523 None:intergenic 20.0%
!!! TGTTGTATAAAATGTTTTTC+TGG + chr3_2:89953734-89953753 Msa0436390:intron 20.0%
!! AAAAATGTCAAAGCATATAT+AGG + chr3_2:89954721-89954740 Msa0436390:CDS 20.0%
!! ATTTATTAGATGGTATATGT+CGG + chr3_2:89954870-89954889 Msa0436390:intron 20.0%
!! TAAGAAATGCTATAATGGAA+AGG - chr3_2:89951318-89951337 None:intergenic 25.0%
! AATTAGTGATTCAGCTATTT+AGG + chr3_2:89951497-89951516 Msa0436390:intron 25.0%
! AGAATATTATGATCTAGATG+AGG + chr3_2:89951682-89951701 Msa0436390:intron 25.0%
! GAATATTATGATCTAGATGA+GGG + chr3_2:89951683-89951702 Msa0436390:intron 25.0%
! AGATACCTATATAATCTTGA+AGG + chr3_2:89951771-89951790 Msa0436390:intron 25.0%
! GTAAACCTTCAAGATTATAT+AGG - chr3_2:89951779-89951798 None:intergenic 25.0%
! CGTCAAAAGAATTAAGATAT+TGG - chr3_2:89951873-89951892 None:intergenic 25.0%
! TCTACATCTTTATGATTCTA+AGG + chr3_2:89951936-89951955 Msa0436390:intron 25.0%
!! AACCAAAAAAGGAAACATTT+TGG - chr3_2:89952015-89952034 None:intergenic 25.0%
!!! AAAATTGCTTTAATTTTGCG+GGG - chr3_2:89952318-89952337 None:intergenic 25.0%
! GAGATAATAATGTGATAAAG+GGG - chr3_2:89952441-89952460 None:intergenic 25.0%
! CAGAGATAATAATGTGATAA+AGG - chr3_2:89952443-89952462 None:intergenic 25.0%
! TGTTCAATAATTTGGGAATT+AGG + chr3_2:89952703-89952722 Msa0436390:intron 25.0%
! GTTCAATAATTTGGGAATTA+GGG + chr3_2:89952704-89952723 Msa0436390:intron 25.0%
!!! ATTAATAATTCTAGGGTTTG+AGG + chr3_2:89952766-89952785 Msa0436390:intron 25.0%
!! TAGGGTTGAAAAATAACTTT+TGG + chr3_2:89952966-89952985 Msa0436390:intron 25.0%
! TAATCCAACACAATAAACAA+TGG - chr3_2:89953045-89953064 None:intergenic 25.0%
! GAACAATAGACATAATAAAG+TGG - chr3_2:89953102-89953121 None:intergenic 25.0%
! AAAACTGCTAATATGTTTGA+GGG + chr3_2:89953205-89953224 Msa0436390:intron 25.0%
! TTGTAAACTTAACTGCTAAT+AGG + chr3_2:89953322-89953341 Msa0436390:intron 25.0%
!! CAAAAATCTTGTGGAATTTT+AGG - chr3_2:89953368-89953387 None:intergenic 25.0%
! TACTATTGACAAAAATCTTG+TGG - chr3_2:89953377-89953396 None:intergenic 25.0%
!! CAAGATTTTTGTCAATAGTA+TGG + chr3_2:89953377-89953396 Msa0436390:intron 25.0%
!!! TTTATTTTTTGTTGCTGTGT+TGG + chr3_2:89953463-89953482 Msa0436390:intron 25.0%
!! AACTTGTTTCACATCTTTAA+CGG + chr3_2:89953759-89953778 Msa0436390:intron 25.0%
! AGTATGAATTTCAACTCAAA+AGG - chr3_2:89953801-89953820 None:intergenic 25.0%
! TTGTGTCTTAAAACTCAAAT+TGG - chr3_2:89953987-89954006 None:intergenic 25.0%
! TTTGACTCATTTACTTTATG+AGG + chr3_2:89954118-89954137 Msa0436390:intron 25.0%
! TCTAATATATCATTCTTACC+TGG + chr3_2:89954164-89954183 Msa0436390:intron 25.0%
! ACTTATCCAAGTAAATTTGT+AGG - chr3_2:89954254-89954273 None:intergenic 25.0%
! TGTATTATTTCGTCAAAGAA+AGG - chr3_2:89954815-89954834 None:intergenic 25.0%
!!! TACATCATGTGACTTTTTTT+TGG + chr3_2:89954831-89954850 Msa0436390:intron 25.0%
! ACATCATGTGATTTATTAGA+TGG + chr3_2:89954860-89954879 Msa0436390:intron 25.0%
!!! TTTTTTTTTTTGCATCAAGC+TGG + chr3_2:89954912-89954931 Msa0436390:intron 25.0%
!! TTTTCATCTATCATTTTGGA+CGG - chr3_2:89954992-89955011 None:intergenic 25.0%
!!! GCTATTTTCATCTATCATTT+TGG - chr3_2:89954996-89955015 None:intergenic 25.0%
!!! TACTTTTTTCCGTAAGTTTT+TGG + chr3_2:89955157-89955176 Msa0436390:intron 25.0%
TAGGCTAAGAAATGCTATAA+TGG - chr3_2:89951323-89951342 None:intergenic 30.0%
GCATACATAAATGGCATAAT+AGG - chr3_2:89951594-89951613 None:intergenic 30.0%
TTGAAGGTTTACTTCAGATA+TGG + chr3_2:89951787-89951806 Msa0436390:intron 30.0%
TAAGGTTGAATTCAACATGT+GGG + chr3_2:89951954-89951973 Msa0436390:intron 30.0%
!!! GTCCAAAATGTTTCCTTTTT+TGG + chr3_2:89952010-89952029 Msa0436390:intron 30.0%
TCACTGTTTCAACTAATTTG+CGG - chr3_2:89952242-89952261 None:intergenic 30.0%
!! AAATTGCTTTAATTTTGCGG+GGG - chr3_2:89952317-89952336 None:intergenic 30.0%
TGTACCATTGTTTATTGTGT+TGG + chr3_2:89953038-89953057 Msa0436390:intron 30.0%
!!! ATTATTTTTTGCTGCTGTGT+TGG + chr3_2:89953061-89953080 Msa0436390:intron 30.0%
!!! TAGTGTGTTTTGATATGCTA+TGG + chr3_2:89953170-89953189 Msa0436390:intron 30.0%
!! ATATGCTATGGTTTTGCTTT+AGG + chr3_2:89953182-89953201 Msa0436390:intron 30.0%
GAAAACTGCTAATATGTTTG+AGG + chr3_2:89953204-89953223 Msa0436390:intron 30.0%
CAGTTAAGTTTACAATGAGA+AGG - chr3_2:89953317-89953336 None:intergenic 30.0%
TTTAGGTATCCACTTGAAAA+AGG - chr3_2:89953351-89953370 None:intergenic 30.0%
! ACTATGTTTTGCATTTGTGT+TGG + chr3_2:89953531-89953550 Msa0436390:intron 30.0%
!!! TAATTCATGTTTTTGGCAGA+TGG + chr3_2:89953662-89953681 Msa0436390:intron 30.0%
GTTTATTCACTATGTCAATC+AGG + chr3_2:89953689-89953708 Msa0436390:intron 30.0%
ACTTGTTTCACATCTTTAAC+GGG + chr3_2:89953760-89953779 Msa0436390:intron 30.0%
!!! GTTTTAAGACACAATTTGTC+AGG + chr3_2:89953993-89954012 Msa0436390:CDS 30.0%
TTGATCATCCAAATGCATAT+TGG + chr3_2:89954020-89954039 Msa0436390:CDS 30.0%
GGTATTCCTACAAATTTACT+TGG + chr3_2:89954245-89954264 Msa0436390:intron 30.0%
TCAAAGAAAGGAACAAAGAT+AGG - chr3_2:89954803-89954822 None:intergenic 30.0%
! TTTCATCTATCATTTTGGAC+GGG - chr3_2:89954991-89955010 None:intergenic 30.0%
AAGTTCACAACAAGTAAAAC+AGG - chr3_2:89955042-89955061 None:intergenic 30.0%
ACTAACATAACGCTCTTTAT+TGG - chr3_2:89955081-89955100 None:intergenic 30.0%
!! TAAAGAGCGTTATGTTAGTT+TGG + chr3_2:89955082-89955101 Msa0436390:intron 30.0%
ACAAAGCTTCCAAAAACTTA+CGG - chr3_2:89955169-89955188 None:intergenic 30.0%
!! TCCCAATATGTTCTGAAATG+AGG + chr3_2:89951558-89951577 Msa0436390:intron 35.0%
CCCTCATTTCAGAACATATT+GGG - chr3_2:89951562-89951581 None:intergenic 35.0%
!! CCCAATATGTTCTGAAATGA+GGG + chr3_2:89951559-89951578 Msa0436390:intron 35.0%
ACCCTCATTTCAGAACATAT+TGG - chr3_2:89951563-89951582 None:intergenic 35.0%
CTAAGGTTGAATTCAACATG+TGG + chr3_2:89951953-89951972 Msa0436390:intron 35.0%
AAGGTTGAATTCAACATGTG+GGG + chr3_2:89951955-89951974 Msa0436390:intron 35.0%
TGTTGGTATTATGTTGAGAG+TGG + chr3_2:89952149-89952168 Msa0436390:intron 35.0%
!! GAATTGAGGAGGTTATTTTG+TGG + chr3_2:89952200-89952219 Msa0436390:intron 35.0%
! ACTGTTTCAACTAATTTGCG+GGG - chr3_2:89952240-89952259 None:intergenic 35.0%
! CACTGTTTCAACTAATTTGC+GGG - chr3_2:89952241-89952260 None:intergenic 35.0%
TGATGGAACAATGAAAGTGA+CGG - chr3_2:89952635-89952654 None:intergenic 35.0%
GTAATTGAATCTGATGACAG+TGG + chr3_2:89953003-89953022 Msa0436390:intron 35.0%
ACCAACAAGACAATTACAGA+AGG + chr3_2:89953277-89953296 Msa0436390:intron 35.0%
ACCTTCTGTAATTGTCTTGT+TGG - chr3_2:89953281-89953300 None:intergenic 35.0%
!!! AATAGGCTTCCTTTTTCAAG+TGG + chr3_2:89953339-89953358 Msa0436390:intron 35.0%
GTATTCAACACCATCTATCA+GGG + chr3_2:89953856-89953875 Msa0436390:CDS 35.0%
TATTCAACACCATCTATCAG+GGG + chr3_2:89953857-89953876 Msa0436390:CDS 35.0%
TAATTAACTCCCCTGATAGA+TGG - chr3_2:89953869-89953888 None:intergenic 35.0%
TAACCAGTCCAATATGCATT+TGG - chr3_2:89954031-89954050 None:intergenic 35.0%
!!! GGACTGGTTATTTTACAAGT+AGG + chr3_2:89954041-89954060 Msa0436390:CDS 35.0%
AGAGCTCGAAATCATTTAAC+AGG - chr3_2:89954147-89954166 None:intergenic 35.0%
ATATATCATTCTTACCTGGC+TGG + chr3_2:89954168-89954187 Msa0436390:intron 35.0%
TATATCATTCTTACCTGGCT+GGG + chr3_2:89954169-89954188 Msa0436390:intron 35.0%
GAACACACACACATTAGATA+TGG + chr3_2:89954224-89954243 Msa0436390:intron 35.0%
ACAGCCAATACACTAAGAAA+AGG + chr3_2:89954412-89954431 Msa0436390:intron 35.0%
! GGTTCCTTTTCTTAGTGTAT+TGG - chr3_2:89954419-89954438 None:intergenic 35.0%
!! TGTTGGTTGGTTTGGTTATT+TGG - chr3_2:89954440-89954459 None:intergenic 35.0%
! CTTTTCTGATATACAACCGA+TGG + chr3_2:89954614-89954633 Msa0436390:CDS 35.0%
!! TTTACTTGTTGTGTCTTCGT+GGG - chr3_2:89954703-89954722 None:intergenic 35.0%
!!! TTTTACTTGTTGTGTCTTCG+TGG - chr3_2:89954704-89954723 None:intergenic 35.0%
ATATAGGATTCCTGATGAAG+TGG + chr3_2:89954737-89954756 Msa0436390:CDS 35.0%
!!! GTTTGGTGCTTTTCTTAGTT+TGG + chr3_2:89955099-89955118 Msa0436390:intron 35.0%
TCCACTGTGAAAACAAGCAT+AGG - chr3_2:89951342-89951361 None:intergenic 40.0%
!! TATGCTTGTTTTCACAGTGG+AGG + chr3_2:89951341-89951360 Msa0436390:CDS 40.0%
TTATGATCTAGATGAGGGAC+AGG + chr3_2:89951688-89951707 Msa0436390:intron 40.0%
TATGATCTAGATGAGGGACA+GGG + chr3_2:89951689-89951708 Msa0436390:intron 40.0%
AGATATTGGCCAGAACTGTT+CGG - chr3_2:89951859-89951878 None:intergenic 40.0%
ATCTCACTACAAAAAACGCG+TGG + chr3_2:89952178-89952197 Msa0436390:intron 40.0%
ACAAAAAACGCGTGGAATTG+AGG + chr3_2:89952186-89952205 Msa0436390:intron 40.0%
!! TTGAGGAGGTTATTTTGTGG+AGG + chr3_2:89952203-89952222 Msa0436390:intron 40.0%
! CTGTTTCAACTAATTTGCGG+GGG - chr3_2:89952239-89952258 None:intergenic 40.0%
CTGCTAATATGTTTGAGGGA+TGG + chr3_2:89953209-89953228 Msa0436390:intron 40.0%
ACTATGTCAATCAGGTACGT+TGG + chr3_2:89953697-89953716 Msa0436390:intron 40.0%
CACATCTTTAACGGGATTTC+TGG + chr3_2:89953768-89953787 Msa0436390:intron 40.0%
GCCATACTTGTATACGATCA+GGG + chr3_2:89953831-89953850 Msa0436390:intron 40.0%
ACCCTGATCGTATACAAGTA+TGG - chr3_2:89953835-89953854 None:intergenic 40.0%
ATACTTGTATACGATCAGGG+TGG + chr3_2:89953834-89953853 Msa0436390:intron 40.0%
GGTATTCAACACCATCTATC+AGG + chr3_2:89953855-89953874 Msa0436390:CDS 40.0%
GGAGCTGTCTAAATTATGTG+TGG - chr3_2:89953966-89953985 None:intergenic 40.0%
CATCCAAATGCATATTGGAC+TGG + chr3_2:89954025-89954044 Msa0436390:CDS 40.0%
! ACAAGTAGGCCAAGCTTTAA+AGG + chr3_2:89954055-89954074 Msa0436390:CDS 40.0%
!! CAAGTAGGCCAAGCTTTAAA+GGG + chr3_2:89954056-89954075 Msa0436390:CDS 40.0%
ATTCATTCTGAGCGTGCTAA+TGG + chr3_2:89954196-89954215 Msa0436390:intron 40.0%
CTTAAGAACATACTTGCCAC+AGG + chr3_2:89954306-89954325 Msa0436390:intron 40.0%
! TTTTTGTCCAGGACTCTCTT+GGG + chr3_2:89954344-89954363 Msa0436390:intron 40.0%
TTATCAGCTTCTGCTCTTGT+TGG - chr3_2:89954457-89954476 None:intergenic 40.0%
!! TTGGTGCTGTTAGTTTGCTT+GGG - chr3_2:89954558-89954577 None:intergenic 40.0%
!! TTTGGTGCTGTTAGTTTGCT+TGG - chr3_2:89954559-89954578 None:intergenic 40.0%
CGGTTGTATATCAGAAAAGC+TGG - chr3_2:89954613-89954632 None:intergenic 40.0%
AAGAAGTTAGCCACTTCATC+AGG - chr3_2:89954750-89954769 None:intergenic 40.0%
GATGAAGTGGCTAACTTCTT+TGG + chr3_2:89954750-89954769 Msa0436390:CDS 40.0%
CACAACAAGTAAAACAGGAC+TGG - chr3_2:89955037-89955056 None:intergenic 40.0%
TCTTCCTTCAAAGGCGAATT+AGG - chr3_2:89955255-89955274 None:intergenic 40.0%
! TAATTCGCCTTTGAAGGAAG+AGG + chr3_2:89955254-89955273 Msa0436390:CDS 40.0%
ACATGATCCTCTTCCTTCAA+AGG - chr3_2:89955264-89955283 None:intergenic 40.0%
TTTGAAGGAAGAGGATCATG+TGG + chr3_2:89955263-89955282 Msa0436390:CDS 40.0%
!! GCCTATGCTTGTTTTCACAG+TGG + chr3_2:89951338-89951357 Msa0436390:CDS 45.0%
! TTTTCACAGTGGAGGCAAGT+TGG + chr3_2:89951349-89951368 Msa0436390:CDS 45.0%
GTAGTTATGAAGCTACCTCG+TGG + chr3_2:89951379-89951398 Msa0436390:CDS 45.0%
CTGCAACATCAGCGCATATT+GGG + chr3_2:89951404-89951423 Msa0436390:CDS 45.0%
TGGGAGTAACATCATCACTG+TGG + chr3_2:89951423-89951442 Msa0436390:CDS 45.0%
TTTGTTGAGCCGAACAGTTC+TGG + chr3_2:89951847-89951866 Msa0436390:intron 45.0%
AAAAACGCGTGGAATTGAGG+AGG + chr3_2:89952189-89952208 Msa0436390:intron 45.0%
! AAGTGAGAGTGTAGCAGGAT+AGG - chr3_2:89952354-89952373 None:intergenic 45.0%
!! AGAAGGTTCTGGATCTGTGA+GGG - chr3_2:89952469-89952488 None:intergenic 45.0%
TAGAGGAAGAGAGTGAGTGT+TGG - chr3_2:89952565-89952584 None:intergenic 45.0%
! GGAACAATGAAAGTGACGGA+GGG - chr3_2:89952631-89952650 None:intergenic 45.0%
TGGAACAATGAAAGTGACGG+AGG - chr3_2:89952632-89952651 None:intergenic 45.0%
CTTTATGAGGGAGACAGTGA+TGG - chr3_2:89952652-89952671 None:intergenic 45.0%
CATCACTGTCTCCCTCATAA+AGG + chr3_2:89952650-89952669 Msa0436390:CDS 45.0%
CGCCATACTTGTATACGATC+AGG + chr3_2:89953830-89953849 Msa0436390:intron 45.0%
!! AACTTTGAAGCGAAGGGGAA+GGG - chr3_2:89953942-89953961 None:intergenic 45.0%
!! TGTGGCAACTTTGAAGCGAA+GGG - chr3_2:89953948-89953967 None:intergenic 45.0%
AGAATGAATAATGCCCAGCC+AGG - chr3_2:89954185-89954204 None:intergenic 45.0%
CTGGACAAAAACCTTCGCAT+TGG - chr3_2:89954335-89954354 None:intergenic 45.0%
! CAATGCGAAGGTTTTTGTCC+AGG + chr3_2:89954333-89954352 Msa0436390:intron 45.0%
! GTTTTTGTCCAGGACTCTCT+TGG + chr3_2:89954343-89954362 Msa0436390:intron 45.0%
! TCTGCTCTTGTTGGTTGGTT+TGG - chr3_2:89954448-89954467 None:intergenic 45.0%
!! ACTGTTTTGTTCTCCTGCTG+GGG - chr3_2:89954586-89954605 None:intergenic 45.0%
!! GACTGTTTTGTTCTCCTGCT+GGG - chr3_2:89954587-89954606 None:intergenic 45.0%
!! TGACTGTTTTGTTCTCCTGC+TGG - chr3_2:89954588-89954607 None:intergenic 45.0%
! TGGTGTTTTGTGCAGGTTAG+CGG - chr3_2:89954660-89954679 None:intergenic 45.0%
!!! CTGGTTTTGGTGTTTTGTGC+AGG - chr3_2:89954667-89954686 None:intergenic 45.0%
AGAGCACGATCAATCTAGTG+TGG + chr3_2:89955125-89955144 Msa0436390:intron 45.0%
TGAGCCTAATTCGCCTTTGA+AGG + chr3_2:89955248-89955267 Msa0436390:CDS 45.0%
GCTGCAACATCAGCGCATAT+TGG + chr3_2:89951403-89951422 Msa0436390:CDS 50.0%
TTGACGTCTTGAGCTCTGCT+TGG + chr3_2:89951887-89951906 Msa0436390:intron 50.0%
TGACGTCTTGAGCTCTGCTT+GGG + chr3_2:89951888-89951907 Msa0436390:intron 50.0%
TCCTGCTACACTCTCACTTG+TGG + chr3_2:89952355-89952374 Msa0436390:intron 50.0%
CCTGCTACACTCTCACTTGT+GGG + chr3_2:89952356-89952375 Msa0436390:intron 50.0%
CTGCTACACTCTCACTTGTG+GGG + chr3_2:89952357-89952376 Msa0436390:intron 50.0%
!! GAGAAGGTTCTGGATCTGTG+AGG - chr3_2:89952470-89952489 None:intergenic 50.0%
GAGAGAGAGTGAGAAGGTTC+TGG - chr3_2:89952480-89952499 None:intergenic 50.0%
GTGAGTGAGAGAGAGTGAGA+AGG - chr3_2:89952486-89952505 None:intergenic 50.0%
!! GAGAGTGAGTGTTGGAACAG+CGG - chr3_2:89952557-89952576 None:intergenic 50.0%
TCTTCCTCTATGACTCTCGC+CGG + chr3_2:89952575-89952594 Msa0436390:CDS 50.0%
! GTGATGGAGTGGTTTGACAC+CGG - chr3_2:89952597-89952616 None:intergenic 50.0%
! AGAGAGTGAGAGTGATGGAG+TGG - chr3_2:89952608-89952627 None:intergenic 50.0%
AATGGGAAGTGAGAGAAGCG+AGG - chr3_2:89953914-89953933 None:intergenic 50.0%
GAAGGGGAAGGGACATGAAT+GGG - chr3_2:89953931-89953950 None:intergenic 50.0%
!! CAACTTTGAAGCGAAGGGGA+AGG - chr3_2:89953943-89953962 None:intergenic 50.0%
!! GTGGCAACTTTGAAGCGAAG+GGG - chr3_2:89953947-89953966 None:intergenic 50.0%
!! GTGTGGCAACTTTGAAGCGA+AGG - chr3_2:89953949-89953968 None:intergenic 50.0%
!! TCTCGCAGCCCTTTAAAGCT+TGG - chr3_2:89954067-89954086 None:intergenic 50.0%
ACCTTCGCATTGGAAACCTG+TGG - chr3_2:89954325-89954344 None:intergenic 50.0%
TTTCCATCCCAAGAGAGTCC+TGG - chr3_2:89954354-89954373 None:intergenic 50.0%
CAGCTTCTGCTCTTGTTGGT+TGG - chr3_2:89954453-89954472 None:intergenic 50.0%
! TTTGGTGTGTGTTAGCTGCC+AGG - chr3_2:89954500-89954519 None:intergenic 50.0%
GCTGTTCTGCTATCTGCCAT+CGG - chr3_2:89954633-89954652 None:intergenic 50.0%
ATGGCAGATAGCAGAACAGC+AGG + chr3_2:89954633-89954652 Msa0436390:CDS 50.0%
!!! TGATCTGGATGGGCTGGTTT+TGG - chr3_2:89954680-89954699 None:intergenic 50.0%
TCTTCGTGGGTGATCTGGAT+GGG - chr3_2:89954690-89954709 None:intergenic 50.0%
TTGTGTCTTCGTGGGTGATC+TGG - chr3_2:89954695-89954714 None:intergenic 50.0%
GAAGGAAGAGGATCATGTGG+TGG + chr3_2:89955266-89955285 Msa0436390:CDS 50.0%
! CATGTGGTGGAAGCAACTTC+TGG + chr3_2:89955279-89955298 Msa0436390:CDS 50.0%
! CCCACAAGTGAGAGTGTAGC+AGG - chr3_2:89952359-89952378 None:intergenic 55.0%
!! ACAGCGGTTCTGGAGAGTCA+CGG - chr3_2:89952541-89952560 None:intergenic 55.0%
!! GAGTGTTGGAACAGCGGTTC+TGG - chr3_2:89952551-89952570 None:intergenic 55.0%
GAGGGAGAGAGTGAGAGTGA+TGG - chr3_2:89952613-89952632 None:intergenic 55.0%
CGAAGGGGAAGGGACATGAA+TGG - chr3_2:89953932-89953951 None:intergenic 55.0%
GCCACAGGTTTCCAATGCGA+AGG + chr3_2:89954321-89954340 Msa0436390:intron 55.0%
TGTCCAGGACTCTCTTGGGA+TGG + chr3_2:89954348-89954367 Msa0436390:intron 55.0%
CACGAGAAACCACTGCATCC+TGG + chr3_2:89954479-89954498 Msa0436390:intron 55.0%
TGTTAGCTGCCAGGATGCAG+TGG - chr3_2:89954491-89954510 None:intergenic 55.0%
TCTGCTGCAGCTGATCGGTT+TGG - chr3_2:89954518-89954537 None:intergenic 55.0%
GTGCTTCTGCTGCAGCTGAT+CGG - chr3_2:89954523-89954542 None:intergenic 55.0%
! TTCTCCTGCTGGGGCTGTTT+TGG - chr3_2:89954577-89954596 None:intergenic 55.0%
GTCTTCGTGGGTGATCTGGA+TGG - chr3_2:89954691-89954710 None:intergenic 55.0%
! GTGGTGGAAGCAACTTCTGG+TGG + chr3_2:89955282-89955301 Msa0436390:CDS 55.0%
! GTGGAGGCAAGTTGGACGTG+CGG + chr3_2:89951357-89951376 Msa0436390:CDS 60.0%
GCTGATGTTGCAGCTCCACG+AGG - chr3_2:89951397-89951416 None:intergenic 60.0%
CGTCTTGAGCTCTGCTTGGG+TGG + chr3_2:89951891-89951910 Msa0436390:intron 60.0%
GCGGCGAGAGTGAGAAAGAG+AGG - chr3_2:89952519-89952538 None:intergenic 60.0%
GACACCGGCGAGAGTCATAG+AGG - chr3_2:89952582-89952601 None:intergenic 60.0%
AGCACCAAAACAGCCCCAGC+AGG + chr3_2:89954570-89954589 Msa0436390:CDS 60.0%
!! GCGGTTCTGGAGAGTCACGG+CGG - chr3_2:89952538-89952557 None:intergenic 65.0%
CGTGGGTGATCTGGATGGGC+TGG - chr3_2:89954686-89954705 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 89951301 89955311 89951301 ID=Msa0436390;Name=Msa0436390
chr3_2 mRNA 89951301 89955311 89951301 ID=Msa0436390-mRNA-1;Parent=Msa0436390;Name=Msa0436390-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|0.8|1|1|5|0|321
chr3_2 exon 89951301 89951455 89951301 ID=Msa0436390-mRNA-1:exon:26715;Parent=Msa0436390-mRNA-1
chr3_2 exon 89952475 89952671 89952475 ID=Msa0436390-mRNA-1:exon:26716;Parent=Msa0436390-mRNA-1
chr3_2 exon 89953852 89954109 89953852 ID=Msa0436390-mRNA-1:exon:26717;Parent=Msa0436390-mRNA-1
chr3_2 exon 89954505 89954782 89954505 ID=Msa0436390-mRNA-1:exon:26718;Parent=Msa0436390-mRNA-1
chr3_2 exon 89955234 89955311 89955234 ID=Msa0436390-mRNA-1:exon:26719;Parent=Msa0436390-mRNA-1
chr3_2 CDS 89951301 89951455 89951301 ID=Msa0436390-mRNA-1:cds;Parent=Msa0436390-mRNA-1
chr3_2 CDS 89952475 89952671 89952475 ID=Msa0436390-mRNA-1:cds;Parent=Msa0436390-mRNA-1
chr3_2 CDS 89953852 89954109 89953852 ID=Msa0436390-mRNA-1:cds;Parent=Msa0436390-mRNA-1
chr3_2 CDS 89954505 89954782 89954505 ID=Msa0436390-mRNA-1:cds;Parent=Msa0436390-mRNA-1
chr3_2 CDS 89955234 89955311 89955234 ID=Msa0436390-mRNA-1:cds;Parent=Msa0436390-mRNA-1
Gene Sequence

>Msa0436390

ATGAGGAATATACTCCTTTCCATTATAGCATTTCTTAGCCTATGCTTGTTTTCACAGTGGAGGCAAGTTGGACGTGCGGTAGTTATGAAGCTACCTCGTGGAGCTGCAACATCAGCGCATATTGGGAGTAACATCATCACTGTGGAAATAAACGTATCCAGAACCTTCTCACTCTCTCTCACTCACAGATTCAGCGCCTCTCTTTCTCACTCTCGCCGCCGTGACTCTCCAGAACCGCTGTTCCAACACTCACTCTCTTCCTCTATGACTCTCGCCGGTGTCAAACCACTCCATCACTCTCACTCTCTCCCTCCGTCACTTTCATTGTTCCATCACTGTCTCCCTCATAAAGGGTGGTATTCAACACCATCTATCAGGGGAGTTAATTATTTTTACAATGACGAAGTGAATCCTCGCTTCTCTCACTTCCCATTCATGTCCCTTCCCCTTCGCTTCAAAGTTGCCACACATAATTTAGACAGCTCCAATTTGAGTTTTAAGACACAATTTGTCAGGTATGTTGATCATCCAAATGCATATTGGACTGGTTATTTTACAAGTAGGCCAAGCTTTAAAGGGCTGCGAGAGAATAATGAGTATTTATTATTAGCTAACACACACCAAACCGATCAGCTGCAGCAGAAGCACAAAACGCAACTACCCAAGCAAACTAACAGCACCAAAACAGCCCCAGCAGGAGAACAAAACAGTCAGACCAGCTTTTCTGATATACAACCGATGGCAGATAGCAGAACAGCAGGACCGCTAACCTGCACAAAACACCAAAACCAGCCCATCCAGATCACCCACGAAGACACAACAAGTAAAAAATGTCAAAGCATATATAGGATTCCTGATGAAGTGGCTAACTTCTTTGGTTCATCTAATACAAATGTTGCAAGTGAGCCTAATTCGCCTTTGAAGGAAGAGGATCATGTGGTGGAAGCAACTTCTGGTGGTTTTTAG
Protein sequence

>Msa0436390

MRNILLSIIAFLSLCLFSQWRQVGRAVVMKLPRGAATSAHIGSNIITVEINVSRTFSLSLTHRFSASLSHSRRRDSPEPLFQHSLSSSMTLAGVKPLHHSHSLPPSLSLFHHCLPHKGWYSTPSIRGVNYFYNDEVNPRFSHFPFMSLPLRFKVATHNLDSSNLSFKTQFVRYVDHPNAYWTGYFTSRPSFKGLRENNEYLLLANTHQTDQLQQKHKTQLPKQTNSTKTAPAGEQNSQTSFSDIQPMADSRTAGPLTCTKHQNQPIQITHEDTTSKKCQSIYRIPDEVANFFGSSNTNVASEPNSPLKEEDHVVEATSGGF*