AlfalfaGEDB | Msa0437580

M. sativa cultivar zhongmu-4 / Msa0437580

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0437580	AES92080.1	82.432	74	13	0	1	74	7	80	1.13e-36	129

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0437580	G7JR13	82.432	74	13	0	1	74	7	80	5.39e-37	129

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0437580	Msa0571700	0.803476	3.422941e-49	-8.615850e-47
Msa0437580	Msa1171870	0.800869	1.177083e-48	-8.615850e-47
Msa0437580	Msa1382410	0.800588	1.343419e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0437580	MtrunA17_Chr4g0069381	82.432	74	13	0	1	74	7	80	1.04e-40	129
Msa0437580	MtrunA17_Chr6g0456701	51.111	90	32	2	1	78	169	258	2.33e-18	78.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 18 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGACGATAAAGTGGCGT+TGG	0.541231	3_3:+1455791	Msa0437580:CDS
AACAGTAAAGGACTTGATGT+TGG	0.517272	3_3:+1455927	Msa0437580:CDS
AAGTTCAGACGATATTGAGC+AGG	0.505825	3_3:+1455950	Msa0437580:CDS
AATACTGACACAACTGAAAA+TGG	0.281796	3_3:-1455889	None:intergenic
AATATTCATGCAAACAGTAA+AGG	0.510920	3_3:+1455915	Msa0437580:CDS
ACCAACTTTGCGAAATGGAT+AGG	0.592428	3_3:+1455838	Msa0437580:CDS
AGACGACCAACTTTGCGAAA+TGG	0.434369	3_3:+1455833	Msa0437580:CDS
AGTTCAGACGATATTGAGCA+GGG	0.655353	3_3:+1455951	Msa0437580:CDS
ATGAGGTTAGTGCTGCAGCT+AGG	0.605201	3_3:+1455739	Msa0437580:intron
CGTCTGTAGCCAATGTTATA+GGG	0.434662	3_3:-1455815	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATACTGACACAACTGAAAA+TGG	-	chr3_3:1455892-1455911	None:intergenic	30.0%
TGTGGAAAATGACGATAAAG+TGG	+	chr3_3:1455785-1455804	Msa0437580:CDS	35.0%
AACAGTAAAGGACTTGATGT+TGG	+	chr3_3:1455927-1455946	Msa0437580:CDS	35.0%
CATTCTCCTCAGGGAAAAAA+GGG	+	chr3_3:1455762-1455781	Msa0437580:CDS	40.0%
AAATGACGATAAAGTGGCGT+TGG	+	chr3_3:1455791-1455810	Msa0437580:CDS	40.0%
CGTCTGTAGCCAATGTTATA+GGG	-	chr3_3:1455818-1455837	None:intergenic	40.0%
TCGTCTGTAGCCAATGTTAT+AGG	-	chr3_3:1455819-1455838	None:intergenic	40.0%
ACCAACTTTGCGAAATGGAT+AGG	+	chr3_3:1455838-1455857	Msa0437580:CDS	40.0%
TTTGCGAAATGGATAGGCTA+CGG	+	chr3_3:1455844-1455863	Msa0437580:CDS	40.0%
GACACAACTGAAAATGGTAG+AGG	-	chr3_3:1455886-1455905	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	1455739	1456022	1455739	ID=Msa0437580;Name=Msa0437580
chr3_3	mRNA	1455739	1456022	1455739	ID=Msa0437580-mRNA-1;Parent=Msa0437580;Name=Msa0437580-mRNA-1;_AED=0.12;_eAED=0.14;_QI=0\|0\|0\|1\|1\|1\|2\|0\|92
chr3_3	exon	1455739	1455743	1455739	ID=Msa0437580-mRNA-1:exon:132;Parent=Msa0437580-mRNA-1
chr3_3	exon	1455749	1456022	1455749	ID=Msa0437580-mRNA-1:exon:133;Parent=Msa0437580-mRNA-1
chr3_3	CDS	1455739	1455743	1455739	ID=Msa0437580-mRNA-1:cds;Parent=Msa0437580-mRNA-1
chr3_3	CDS	1455749	1456022	1455749	ID=Msa0437580-mRNA-1:cds;Parent=Msa0437580-mRNA-1

Gene Sequence	>Msa0437580 ATGAGTGCTGCAGCTAGGCATTCTCCTCAGGGAAAAAAGGGTGTGGAAAATGACGATAAAGTGGCGTTGGACCCTATAACATTGGCTACAGACGACCAACTTTGCGAAATGGATAGGCTACGGACTAATGCTGCAGTTACCTCTACCATTTTCAGTTGTGTCAGTATTGACAATATTCATGCAAACAGTAAAGGACTTGATGTTGGAAGTTCAGACGATATTGAGCAGGGCAGAAAGTTTTGCATACTAAAGAAAAAAGATATACAATCATGCAATTGA
Protein sequence	>Msa0437580 MSAAARHSPQGKKGVENDDKVALDPITLATDDQLCEMDRLRTNAAVTSTIFSCVSIDNIHANSKGLDVGSSDDIEQGRKFCILKKKDIQSCN*