AlfalfaGEDB | Msa0444450

M. sativa cultivar zhongmu-4 / Msa0444450

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444450	AES68952.1	88.272	162	19	0	31	192	662	823	1.50e-93	300

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444450	sp\|O81833\|SD11_ARATH	48.619	181	88	2	16	192	636	815	5.69e-48	170

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444450	G7IYX8	88.272	162	19	0	31	192	662	823	7.15e-94	300

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0350210	Msa0444450	0.807785	4.266603e-50	-8.615850e-47
Msa0397180	Msa0444450	0.808918	2.445265e-50	-8.615850e-47
Msa0444450	Msa1027910	0.834215	3.409518e-56	-8.615850e-47
Msa0444450	Msa1084890	0.836332	9.955052e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444450	MtrunA17_Chr3g0084441	88.272	162	19	0	31	192	662	823	1.38e-97	300
Msa0444450	MtrunA17_Chr3g0084491	87.654	162	20	0	31	192	662	823	1.58e-95	295
Msa0444450	MtrunA17_Chr3g0084451	84.568	162	25	0	31	192	662	823	2.72e-92	286
Msa0444450	MtrunA17_Chr3g0084691	76.271	177	36	2	16	192	666	836	1.67e-84	266
Msa0444450	MtrunA17_Chr3g0084411	75.275	182	36	3	16	192	643	820	1.93e-84	265
Msa0444450	MtrunA17_Chr3g0098781	72.472	178	44	3	16	192	55	228	1.70e-82	244
Msa0444450	MtrunA17_Chr3g0084681	75.309	162	36	1	31	192	620	777	2.11e-80	254
Msa0444450	MtrunA17_Chr3g0084631	68.927	177	47	3	16	192	7	175	7.56e-80	235
Msa0444450	MtrunA17_Chr3g0084421	73.626	182	39	3	16	192	606	783	1.25e-79	252
Msa0444450	MtrunA17_Chr3g0084661	70.621	177	44	3	16	192	177	345	6.75e-79	239

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0444450	AT4G27300.1	48.619	181	88	2	16	192	636	815	5.78e-49	170
Msa0444450	AT4G27290.2	50.658	152	73	1	43	192	678	829	6.39e-41	147
Msa0444450	AT4G27290.1	50.658	152	73	1	43	192	632	783	7.27e-41	147
Msa0444450	AT4G00960.2	41.304	184	98	4	16	192	105	285	1.05e-40	139
Msa0444450	AT4G00960.3	41.304	184	98	4	16	192	112	292	1.62e-40	139
Msa0444450	AT1G65790.1	44.022	184	86	4	17	192	669	843	7.55e-40	144
Msa0444450	AT4G00960.1	41.304	184	98	4	16	192	199	379	8.77e-40	139
Msa0444450	AT4G23150.1	45.882	170	83	4	31	192	491	659	1.20e-39	143
Msa0444450	AT1G65800.1	43.478	184	87	4	17	192	673	847	1.57e-39	143
Msa0444450	AT4G21380.1	44.022	184	86	4	17	192	676	850	3.39e-39	142

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Find 40 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCACAGATGACATGTT+AGG	0.562151	3_3:+17395747	None:intergenic
AACCCGGGTTCATTTGGTTT+TGG	0.229082	3_3:+17395699	None:intergenic
AACCCGGGTTCTACACTGCA+AGG	0.565764	3_3:-17395686	Msa0444450:CDS
AAGAACAAGGGTTACGATTT+TGG	0.168974	3_3:-17397560	Msa0444450:CDS
AATTAAAACATACATAAACT+GGG	0.643301	3_3:+17397475	None:intergenic
ACACGGAGTGGCATGCTATC+GGG	0.500756	3_3:+17395590	None:intergenic
ACCCGGGTTCTACACTGCAA+GGG	0.679536	3_3:-17395685	Msa0444450:CDS
AGAAGGTTAAGACGATGTAG+AGG	0.611325	3_3:+17396172	None:intergenic
ATAATAAGATTTATTCATGT+TGG	0.464265	3_3:-17395804	Msa0444450:CDS
ATCCCTTGCAGTGTAGAACC+CGG	0.517879	3_3:+17395683	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGCTAAATCTCTTAGAGATT+AGG	-	chr3_3:17395782-17395801	Msa0444450:CDS	30.0%
ATGATCAATTAGGGTTACAT+AGG	+	chr3_3:17395819-17395838	None:intergenic	30.0%
CAGCAACATATGATCAATTA+GGG	+	chr3_3:17395828-17395847	None:intergenic	30.0%
ACAGCAACATATGATCAATT+AGG	+	chr3_3:17395829-17395848	None:intergenic	30.0%
GTTGTACTTGAGATAATTAG+CGG	-	chr3_3:17396975-17396994	Msa0444450:intron	30.0%
AGACCAAAAAAAGGGATATA+TGG	+	chr3_3:17397119-17397138	None:intergenic	30.0%
TCTCAATAAATCTTCTGCAT+GGG	-	chr3_3:17397136-17397155	Msa0444450:intron	30.0%
CTCATCAATCACTTATAAAG+TGG	+	chr3_3:17397257-17397276	None:intergenic	30.0%
AGCTAAATCTCTTAGAGATT+AGG	-	chr3_3:17395782-17395801	Msa0444450:CDS	30.0%
ATGATCAATTAGGGTTACAT+AGG	+	chr3_3:17395819-17395838	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	17395605	17397612	17395605	ID=Msa0444450;Name=Msa0444450
chr3_3	mRNA	17395605	17397612	17395605	ID=Msa0444450-mRNA-1;Parent=Msa0444450;Name=Msa0444450-mRNA-1;_AED=0.55;_eAED=0.55;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|192
chr3_3	exon	17397488	17397612	17397488	ID=Msa0444450-mRNA-1:exon:3738;Parent=Msa0444450-mRNA-1
chr3_3	exon	17396165	17396315	17396165	ID=Msa0444450-mRNA-1:exon:3737;Parent=Msa0444450-mRNA-1
chr3_3	exon	17395605	17395907	17395605	ID=Msa0444450-mRNA-1:exon:3736;Parent=Msa0444450-mRNA-1
chr3_3	CDS	17397488	17397612	17397488	ID=Msa0444450-mRNA-1:cds;Parent=Msa0444450-mRNA-1
chr3_3	CDS	17396165	17396315	17396165	ID=Msa0444450-mRNA-1:cds;Parent=Msa0444450-mRNA-1
chr3_3	CDS	17395605	17395907	17395605	ID=Msa0444450-mRNA-1:cds;Parent=Msa0444450-mRNA-1

Gene Sequence

>Msa0444450

ATGACTTCAATAAATACTGCACTCAATCTGAAGAACAAGGGTTACGATTTTGGAGAACGAAAGTGCGATTTTAACACCCAGAAGGCTGCAGCGGAGAGGATTTCATCATCTCTAACCCAGTTTATTGGATATATGCCTCCAGAATATGCAGCGGATGGATCCTTCTCAATAAAATCTGATGTTTTTAGCTTTGGAGTCGTTGTACTTGAGATAATTAGCGGTAGAAAGAATCACGGATTTCGTGACCCTCTACATCGTCTTAACCTTCTTGGTCATGCATGGAGACTTTGGATTGAAGGAAGGCCACTGGATTTAATAGCTGACATATTATATGATGATGAGGCCATATGTTCAGAAATAATAAGATTTATTCATGTTGGTCTATTGTGTGTTCAACAGCTACCAGAAAATAGGCCTAACATGTCATCTGTGGTTTTTATGTTAAAGGGTGAAAAATTACTCCCAAAACCAAATGAACCCGGGTTCTACACTGCAAGGGATAATACAAATAGCATAGAATCTTCATCTAAAGAGTTTTCAATAAATGAAGCTTCAATCTCATTGTTAGACGCCCGATAG

Protein sequence

>Msa0444450

MTSINTALNLKNKGYDFGERKCDFNTQKAAAERISSSLTQFIGYMPPEYAADGSFSIKSDVFSFGVVVLEIISGRKNHGFRDPLHRLNLLGHAWRLWIEGRPLDLIADILYDDEAICSEIIRFIHVGLLCVQQLPENRPNMSSVVFMLKGEKLLPKPNEPGFYTARDNTNSIESSSKEFSINEASISLLDAR*