AlfalfaGEDB | Msa0476060

M. sativa cultivar zhongmu-4 / Msa0476060

KEGG: K09338
GO: GO:0001101 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009735 GO:0009737 GO:0009738 GO:0009755 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0033993 GO:0042221 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0080090 GO:0097305 GO:0097306 GO:0140110 GO:1901700 GO:1901701 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0476060	XP_013461831.1	83.828	303	30	5	1	294	12	304	2.77e-151	436

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0476060	sp\|Q8GRL4\|HOX19_ORYSJ	53.591	181	63	3	83	248	81	255	9.38e-54	179

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0476060	A0A072V2J8	83.828	303	30	5	1	294	12	304	1.33e-151	436

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TFs/TRs:

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Gene ID	Type	Classification
Msa0476060	TF	HB-HD-ZIP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0381460	Msa0476060	0.821916	3.130795e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0476060	MtrunA17_Chr3g0135331	83.828	303	30	5	1	294	12	304	2.55e-155	436
Msa0476060	MtrunA17_Chr1g0151971	49.147	293	123	11	6	290	11	285	3.25e-73	226
Msa0476060	MtrunA17_Chr5g0400781	59.509	163	59	4	124	286	114	269	5.98e-57	184
Msa0476060	MtrunA17_Chr7g0258831	69.048	126	36	2	123	248	150	272	3.02e-54	178
Msa0476060	MtrunA17_Chr1g0175651	50.249	201	90	4	50	250	78	268	1.33e-53	176
Msa0476060	MtrunA17_Chr4g0054421	64.567	127	42	2	122	248	116	239	1.66e-51	170
Msa0476060	MtrunA17_Chr2g0308611	64.341	129	43	2	124	252	191	316	1.38e-49	167
Msa0476060	MtrunA17_Chr4g0055851	54.305	151	65	2	123	272	110	257	1.02e-48	163
Msa0476060	MtrunA17_Chr5g0399491	56.944	144	58	2	123	265	126	266	1.45e-48	163
Msa0476060	MtrunA17_Chr8g0335131	67.521	117	34	2	132	247	145	258	1.28e-47	161

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0476060	AT5G06710.1	57.576	165	62	4	89	253	151	307	5.33e-54	179
Msa0476060	AT4G37790.1	67.717	127	38	2	122	248	115	238	7.15e-53	174
Msa0476060	AT2G22800.1	67.200	125	38	2	124	248	104	225	7.00e-51	169
Msa0476060	AT4G16780.1	40.385	260	112	4	42	266	9	260	1.30e-50	169
Msa0476060	AT3G60390.1	62.992	127	43	2	122	247	151	274	2.42e-48	164
Msa0476060	AT5G47370.1	61.719	128	45	2	122	248	119	243	8.17e-47	159
Msa0476060	AT4G17460.1	61.905	126	44	2	124	248	126	248	1.30e-46	158
Msa0476060	AT2G44910.1	63.248	117	41	1	132	248	162	276	3.44e-45	155
Msa0476060	AT2G01430.1	55.639	133	48	3	122	248	122	249	2.25e-40	142
Msa0476060	AT1G70920.2	46.939	147	66	3	121	266	26	161	4.32e-35	125

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Find 83 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATTTGCACCGGCAC+CGG	0.514546	3_3:-79784185	Msa0476060:CDS
AAAGCTGAAACAAACGGAAG+TGG	0.530773	3_3:-79784366	Msa0476060:CDS
AAATAATTACACGCAGCTTC+CGG	0.391339	3_3:-79784237	Msa0476060:CDS
AACAGAAGAGAACAAGAGGC+TGG	0.492600	3_3:-79784309	Msa0476060:CDS
AAGAGAAATATCAAAATTCA+TGG	0.395654	3_3:+79785820	None:intergenic
AAGCCTAAGTTTCTTCCTAC+TGG	0.393408	3_3:+79785439	None:intergenic
AATCAAAGCCGTGGTGTGAG+AGG	0.529506	3_3:+79784142	None:intergenic
ACAAACGGAAGTGGACTGTG+AGG	0.696543	3_3:-79784357	Msa0476060:CDS
ACAAAGCAAGAAGATTAAGG+AGG	0.671987	3_3:-79785762	Msa0476060:CDS
ACCGGCACCGGCGATGAGAA+TGG	0.503337	3_3:-79784167	Msa0476060:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGATATTTCTCTTGGTCT+TGG	-	chr3_3:79783839-79783858	Msa0476060:three_prime_UTR	30.0%
GTTTATGTCTTTGTTCATGA+AGG	+	chr3_3:79783871-79783890	None:intergenic	30.0%
TACTACAACAAAGACTGAAT+CGG	-	chr3_3:79784015-79784034	Msa0476060:three_prime_UTR	30.0%
TCAACAAATGAAATCTTCTC+TGG	+	chr3_3:79784169-79784188	None:intergenic	30.0%
ATGTAAATGATAACTCCAGT+AGG	-	chr3_3:79784197-79784216	Msa0476060:CDS	30.0%
CTTTACAAGGACTAAATACA+TGG	+	chr3_3:79784553-79784572	None:intergenic	30.0%
TCTTGTAAAAGAATTTGCAC+CGG	-	chr3_3:79785460-79785479	Msa0476060:CDS	30.0%
ATAGCCGATTATTGAATATG+AGG	-	chr3_3:79785712-79785731	Msa0476060:CDS	30.0%
CGATCCTCATATTCAATAAT+CGG	+	chr3_3:79785719-79785738	None:intergenic	30.0%
CGATTATTGAATATGAGGAT+CGG	-	chr3_3:79785717-79785736	Msa0476060:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	79783723	79785950	79783723	ID=Msa0476060;Name=Msa0476060
chr3_3	mRNA	79783723	79785950	79783723	ID=Msa0476060-mRNA-1;Parent=Msa0476060;Name=Msa0476060-mRNA-1;_AED=0.01;_eAED=0.01;_QI=101\|1\|1\|1\|1\|1\|3\|335\|294
chr3_3	exon	79783723	79784391	79783723	ID=Msa0476060-mRNA-1:exon:21635;Parent=Msa0476060-mRNA-1
chr3_3	exon	79784868	79784947	79784868	ID=Msa0476060-mRNA-1:exon:21634;Parent=Msa0476060-mRNA-1
chr3_3	exon	79785379	79785950	79785379	ID=Msa0476060-mRNA-1:exon:21633;Parent=Msa0476060-mRNA-1
chr3_3	five_prime_UTR	79785850	79785950	79785850	ID=Msa0476060-mRNA-1:five_prime_utr;Parent=Msa0476060-mRNA-1
chr3_3	CDS	79785379	79785849	79785379	ID=Msa0476060-mRNA-1:cds;Parent=Msa0476060-mRNA-1
chr3_3	CDS	79784868	79784947	79784868	ID=Msa0476060-mRNA-1:cds;Parent=Msa0476060-mRNA-1
chr3_3	CDS	79784058	79784391	79784058	ID=Msa0476060-mRNA-1:cds;Parent=Msa0476060-mRNA-1
chr3_3	three_prime_UTR	79783723	79784057	79783723	ID=Msa0476060-mRNA-1:three_prime_utr;Parent=Msa0476060-mRNA-1

Gene Sequence

>Msa0476060

ATGAACACCATGAATTTTGATATTTCTCTTGGTCTTGGTCGTGGCCTTCATGAACAAAGACATAAACAAAGCAAGAAGATTAAGGAGGAAGAGGAGGATCCATGTAATAAGGCATACCCATATTCTCTTACCTTAGGAGTTGGATCCCCTAATAATAATATTGAAGAGACAGATAATCATCAAGTAGCTACTACTACAACAAAGACTGAATCGGATGAGTTTATTTATAATTTGCATCAACGTGCCTCTAATTCTACTAGCGTGATGTCTTCATTTTCCAACTCCTCTAATAGCATCAAGAGGGAGAGAAATGATTTCAACAGCCAAGAAGTACATCATGAGCCAGAGAAGATTTCATTTGTTGATGCTGATGATGTAAATGATAACTCCAGTAGGAAGAAACTTAGGCTTACCAAAGAACAATCCTCAATCTTGGAGGACACCTTCAAAGATCATTGTACTCTCAATCCGAAGCAAAAGCAAGAATTGGCAAGCAAGCTGAATCTTCGGACGAGACAAGTGGAGGTGTGGTTTCAAAACAGGAGGGCAAGGACAAAGCTGAAACAAACGGAAGTGGACTGTGAGGCACTGAAGCATTGTTATGAAACTCTAACAGAAGAGAACAAGAGGCTGGAAGAGGAGTTGAAAGAGCTTAAGTCCATGAAAACAACGGCACAACCAGTAAATAATTACACGCAGCTTCCGGTTGCCAGTCTTACAGTTTGCCCTTCTTGTAAAAGAATTTGCACCGGCACCGGCACCGGCGATGAGAATGGCTCCTCTCACACCACGGCTTTGATTCTCTGCCCAAAGGCCCAAATTCACTTCTATGCAAATAATAATAACTATACGTTCGCCAAATCTTCTGCAACTATTGCTAGCTAG

Protein sequence

>Msa0476060

MNTMNFDISLGLGRGLHEQRHKQSKKIKEEEEDPCNKAYPYSLTLGVGSPNNNIEETDNHQVATTTTKTESDEFIYNLHQRASNSTSVMSSFSNSSNSIKRERNDFNSQEVHHEPEKISFVDADDVNDNSSRKKLRLTKEQSSILEDTFKDHCTLNPKQKQELASKLNLRTRQVEVWFQNRRARTKLKQTEVDCEALKHCYETLTEENKRLEEELKELKSMKTTAQPVNNYTQLPVASLTVCPSCKRICTGTGTGDENGSSHTTALILCPKAQIHFYANNNNYTFAKSSATIAS*