Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0473770 | Msa0517790 | 0.855043 | 8.217684e-62 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAATCTCTTGTGAATATT+TGG | 0.173679 | 3_4:-69512043 | Msa0517790:CDS |
| CTCTGGAACTGAAGAACTTC+AGG | 0.337569 | 3_4:+69512910 | None:intergenic |
| ATACCTTGTGATCATATCTC+TGG | 0.462832 | 3_4:+69512893 | None:intergenic |
| TTTCTAAGAGGAAAATCGTT+GGG | 0.473698 | 3_4:-69512074 | Msa0517790:CDS |
| CTTGTGAATATTTGGCATTG+AGG | 0.500553 | 3_4:-69512035 | Msa0517790:CDS |
| TATTTGCATAGCTTTCTAAG+AGG | 0.508660 | 3_4:-69512086 | Msa0517790:intron |
| CAAGTTTAGTAGAAAGCGAT+GGG | 0.510504 | 3_4:+69512007 | None:intergenic |
| TTCACAAGAGATTGCTGTCA+AGG | 0.537305 | 3_4:+69512050 | None:intergenic |
| CTTTCTAAGAGGAAAATCGT+TGG | 0.572401 | 3_4:-69512075 | Msa0517790:CDS |
| TCAAGTTTAGTAGAAAGCGA+TGG | 0.574833 | 3_4:+69512006 | None:intergenic |
| GTTCCAGAGATATGATCACA+AGG | 0.686583 | 3_4:-69512896 | Msa0517790:intron |
| AAGTTTAGTAGAAAGCGATG+GGG | 0.745162 | 3_4:+69512008 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTACTTGATAATAATTAC+AGG | - | chr3_4:69512386-69512405 | Msa0517790:intron | 15.0% |
| !! | AATAAAATTCATCAAAAGAT+AGG | + | chr3_4:69512789-69512808 | None:intergenic | 15.0% |
| !!! | ATTTACTTGATAATAATTAC+AGG | - | chr3_4:69512386-69512405 | Msa0517790:intron | 15.0% |
| !! | AATAAAATTCATCAAAAGAT+AGG | + | chr3_4:69512789-69512808 | None:intergenic | 15.0% |
| !! | TAATATTCAATAAGTTCAAG+TGG | - | chr3_4:69512053-69512072 | Msa0517790:CDS | 20.0% |
| !! | TTATATACTATGTTGTGATA+TGG | - | chr3_4:69512108-69512127 | Msa0517790:intron | 20.0% |
| !! | AAACAAGCAAGTAAAATTAA+AGG | - | chr3_4:69512137-69512156 | Msa0517790:intron | 20.0% |
| !!! | TGAAAGTGTTAATGAATTTT+TGG | + | chr3_4:69512341-69512360 | None:intergenic | 20.0% |
| !! | ACTTTCAACATTAATTAACT+TGG | - | chr3_4:69512354-69512373 | Msa0517790:intron | 20.0% |
| !! | ATATTAAAAATGCTGATGTT+TGG | - | chr3_4:69512546-69512565 | Msa0517790:intron | 20.0% |
| !! | TAATATTCAATAAGTTCAAG+TGG | - | chr3_4:69512053-69512072 | Msa0517790:CDS | 20.0% |
| !! | TTATATACTATGTTGTGATA+TGG | - | chr3_4:69512108-69512127 | Msa0517790:intron | 20.0% |
| !! | AAACAAGCAAGTAAAATTAA+AGG | - | chr3_4:69512137-69512156 | Msa0517790:intron | 20.0% |
| !!! | TGAAAGTGTTAATGAATTTT+TGG | + | chr3_4:69512341-69512360 | None:intergenic | 20.0% |
| !! | ACTTTCAACATTAATTAACT+TGG | - | chr3_4:69512354-69512373 | Msa0517790:intron | 20.0% |
| !! | ATATTAAAAATGCTGATGTT+TGG | - | chr3_4:69512546-69512565 | Msa0517790:intron | 20.0% |
| ! | AAATACAACAGAAATCAAGA+AGG | + | chr3_4:69512031-69512050 | None:intergenic | 25.0% |
| !! | AAATCTGGAATAGAAACAAA+CGG | + | chr3_4:69512297-69512316 | None:intergenic | 25.0% |
| ! | TTAATATCAACAGTAATCGT+AGG | + | chr3_4:69512533-69512552 | None:intergenic | 25.0% |
| !!! | TTTTTTCACTCAATTACTTG+TGG | - | chr3_4:69512710-69512729 | Msa0517790:intron | 25.0% |
| ! | AAATACAACAGAAATCAAGA+AGG | + | chr3_4:69512031-69512050 | None:intergenic | 25.0% |
| !! | AAATCTGGAATAGAAACAAA+CGG | + | chr3_4:69512297-69512316 | None:intergenic | 25.0% |
| ! | TTAATATCAACAGTAATCGT+AGG | + | chr3_4:69512533-69512552 | None:intergenic | 25.0% |
| !!! | TTTTTTCACTCAATTACTTG+TGG | - | chr3_4:69512710-69512729 | Msa0517790:intron | 25.0% |
| ATTTAGAAGCTCAAAAAGCA+TGG | + | chr3_4:69512433-69512452 | None:intergenic | 30.0% | |
| !! | ATGCTTTTTGAGCTTCTAAA+TGG | - | chr3_4:69512432-69512451 | Msa0517790:intron | 30.0% |
| ! | AATGGTTATCCACCTTTTAA+TGG | - | chr3_4:69512450-69512469 | Msa0517790:intron | 30.0% |
| TAAAGAATCGGTTGAGAAAA+TGG | + | chr3_4:69512604-69512623 | None:intergenic | 30.0% | |
| !! | TTTTCTCAACCGATTCTTTA+TGG | - | chr3_4:69512604-69512623 | Msa0517790:intron | 30.0% |
| TCTTGAACAAATATCAAGAC+AGG | + | chr3_4:69512641-69512660 | None:intergenic | 30.0% | |
| TATTTGCATAGCTTTCTAAG+AGG | - | chr3_4:69512805-69512824 | Msa0517790:intron | 30.0% | |
| ! | TTTCTAAGAGGAAAATCGTT+GGG | - | chr3_4:69512817-69512836 | Msa0517790:intron | 30.0% |
| AGCAATCTCTTGTGAATATT+TGG | - | chr3_4:69512848-69512867 | Msa0517790:intron | 30.0% | |
| ATTTAGAAGCTCAAAAAGCA+TGG | + | chr3_4:69512433-69512452 | None:intergenic | 30.0% | |
| !! | ATGCTTTTTGAGCTTCTAAA+TGG | - | chr3_4:69512432-69512451 | Msa0517790:intron | 30.0% |
| ! | AATGGTTATCCACCTTTTAA+TGG | - | chr3_4:69512450-69512469 | Msa0517790:intron | 30.0% |
| TAAAGAATCGGTTGAGAAAA+TGG | + | chr3_4:69512604-69512623 | None:intergenic | 30.0% | |
| !! | TTTTCTCAACCGATTCTTTA+TGG | - | chr3_4:69512604-69512623 | Msa0517790:intron | 30.0% |
| TCTTGAACAAATATCAAGAC+AGG | + | chr3_4:69512641-69512660 | None:intergenic | 30.0% | |
| TATTTGCATAGCTTTCTAAG+AGG | - | chr3_4:69512805-69512824 | Msa0517790:intron | 30.0% | |
| ! | TTTCTAAGAGGAAAATCGTT+GGG | - | chr3_4:69512817-69512836 | Msa0517790:intron | 30.0% |
| AGCAATCTCTTGTGAATATT+TGG | - | chr3_4:69512848-69512867 | Msa0517790:intron | 30.0% | |
| ATACCTTGTGATCATATCTC+TGG | + | chr3_4:69512001-69512020 | None:intergenic | 35.0% | |
| TTATTGTTGTGCACACGAAT+CGG | + | chr3_4:69512226-69512245 | None:intergenic | 35.0% | |
| CCTAATATGACTACGAAATC+TGG | + | chr3_4:69512312-69512331 | None:intergenic | 35.0% | |
| CCAGATTTCGTAGTCATATT+AGG | - | chr3_4:69512309-69512328 | Msa0517790:intron | 35.0% | |
| AATAATTACAGGCAGACATG+TGG | - | chr3_4:69512397-69512416 | Msa0517790:intron | 35.0% | |
| TTGTTTCAGCCATTAAAAGG+TGG | + | chr3_4:69512462-69512481 | None:intergenic | 35.0% | |
| ! | ACGTTGTTTCAGCCATTAAA+AGG | + | chr3_4:69512465-69512484 | None:intergenic | 35.0% |
| ! | TCAACCGATTCTTTATGGAT+TGG | - | chr3_4:69512609-69512628 | Msa0517790:intron | 35.0% |
| GAGTCCAATCCATAAAGAAT+CGG | + | chr3_4:69512616-69512635 | None:intergenic | 35.0% | |
| ! | CTTTCTAAGAGGAAAATCGT+TGG | - | chr3_4:69512816-69512835 | Msa0517790:intron | 35.0% |
| CTTGTGAATATTTGGCATTG+AGG | - | chr3_4:69512856-69512875 | Msa0517790:intron | 35.0% | |
| AAGTTTAGTAGAAAGCGATG+GGG | + | chr3_4:69512886-69512905 | None:intergenic | 35.0% | |
| CAAGTTTAGTAGAAAGCGAT+GGG | + | chr3_4:69512887-69512906 | None:intergenic | 35.0% | |
| TCAAGTTTAGTAGAAAGCGA+TGG | + | chr3_4:69512888-69512907 | None:intergenic | 35.0% | |
| ATACCTTGTGATCATATCTC+TGG | + | chr3_4:69512001-69512020 | None:intergenic | 35.0% | |
| TTATTGTTGTGCACACGAAT+CGG | + | chr3_4:69512226-69512245 | None:intergenic | 35.0% | |
| CCTAATATGACTACGAAATC+TGG | + | chr3_4:69512312-69512331 | None:intergenic | 35.0% | |
| CCAGATTTCGTAGTCATATT+AGG | - | chr3_4:69512309-69512328 | Msa0517790:intron | 35.0% | |
| AATAATTACAGGCAGACATG+TGG | - | chr3_4:69512397-69512416 | Msa0517790:intron | 35.0% | |
| TTGTTTCAGCCATTAAAAGG+TGG | + | chr3_4:69512462-69512481 | None:intergenic | 35.0% | |
| ! | ACGTTGTTTCAGCCATTAAA+AGG | + | chr3_4:69512465-69512484 | None:intergenic | 35.0% |
| ! | TCAACCGATTCTTTATGGAT+TGG | - | chr3_4:69512609-69512628 | Msa0517790:intron | 35.0% |
| GAGTCCAATCCATAAAGAAT+CGG | + | chr3_4:69512616-69512635 | None:intergenic | 35.0% | |
| ! | CTTTCTAAGAGGAAAATCGT+TGG | - | chr3_4:69512816-69512835 | Msa0517790:intron | 35.0% |
| CTTGTGAATATTTGGCATTG+AGG | - | chr3_4:69512856-69512875 | Msa0517790:intron | 35.0% | |
| AAGTTTAGTAGAAAGCGATG+GGG | + | chr3_4:69512886-69512905 | None:intergenic | 35.0% | |
| CAAGTTTAGTAGAAAGCGAT+GGG | + | chr3_4:69512887-69512906 | None:intergenic | 35.0% | |
| TCAAGTTTAGTAGAAAGCGA+TGG | + | chr3_4:69512888-69512907 | None:intergenic | 35.0% | |
| GTTCCAGAGATATGATCACA+AGG | - | chr3_4:69511995-69512014 | Msa0517790:CDS | 40.0% | |
| AGTTCAAGTGGCACATAAGA+TGG | - | chr3_4:69512065-69512084 | Msa0517790:CDS | 40.0% | |
| ATCGCTTGCGCTAAAAGAAT+TGG | - | chr3_4:69512162-69512181 | Msa0517790:intron | 40.0% | |
| TCGCTTGCGCTAAAAGAATT+GGG | - | chr3_4:69512163-69512182 | Msa0517790:intron | 40.0% | |
| TAATGGCTGAAACAACGTTC+AGG | - | chr3_4:69512467-69512486 | Msa0517790:intron | 40.0% | |
| AGAAAATGGAAGACAGGTAC+GGG | + | chr3_4:69512590-69512609 | None:intergenic | 40.0% | |
| GAGAAAATGGAAGACAGGTA+CGG | + | chr3_4:69512591-69512610 | None:intergenic | 40.0% | |
| TTCACAAGAGATTGCTGTCA+AGG | + | chr3_4:69512844-69512863 | None:intergenic | 40.0% | |
| GTTCCAGAGATATGATCACA+AGG | - | chr3_4:69511995-69512014 | Msa0517790:CDS | 40.0% | |
| AGTTCAAGTGGCACATAAGA+TGG | - | chr3_4:69512065-69512084 | Msa0517790:CDS | 40.0% | |
| ATCGCTTGCGCTAAAAGAAT+TGG | - | chr3_4:69512162-69512181 | Msa0517790:intron | 40.0% | |
| TCGCTTGCGCTAAAAGAATT+GGG | - | chr3_4:69512163-69512182 | Msa0517790:intron | 40.0% | |
| TAATGGCTGAAACAACGTTC+AGG | - | chr3_4:69512467-69512486 | Msa0517790:intron | 40.0% | |
| AGAAAATGGAAGACAGGTAC+GGG | + | chr3_4:69512590-69512609 | None:intergenic | 40.0% | |
| GAGAAAATGGAAGACAGGTA+CGG | + | chr3_4:69512591-69512610 | None:intergenic | 40.0% | |
| TTCACAAGAGATTGCTGTCA+AGG | + | chr3_4:69512844-69512863 | None:intergenic | 40.0% | |
| !! | CTCTGGAACTGAAGAACTTC+AGG | + | chr3_4:69511984-69512003 | None:intergenic | 45.0% |
| CACACGAATCGGAAAATCAG+AGG | + | chr3_4:69512215-69512234 | None:intergenic | 45.0% | |
| !! | TCATTTTTCTCACGTTCCCG+TGG | - | chr3_4:69512256-69512275 | Msa0517790:intron | 45.0% |
| ! | CATTTTTCTCACGTTCCCGT+GGG | - | chr3_4:69512257-69512276 | Msa0517790:intron | 45.0% |
| CGGTTGAGAAAATGGAAGAC+AGG | + | chr3_4:69512596-69512615 | None:intergenic | 45.0% | |
| GTATTTGCACCTTGTTGCAG+TGG | - | chr3_4:69512759-69512778 | Msa0517790:intron | 45.0% | |
| !! | CTCTGGAACTGAAGAACTTC+AGG | + | chr3_4:69511984-69512003 | None:intergenic | 45.0% |
| CACACGAATCGGAAAATCAG+AGG | + | chr3_4:69512215-69512234 | None:intergenic | 45.0% | |
| !! | TCATTTTTCTCACGTTCCCG+TGG | - | chr3_4:69512256-69512275 | Msa0517790:intron | 45.0% |
| ! | CATTTTTCTCACGTTCCCGT+GGG | - | chr3_4:69512257-69512276 | Msa0517790:intron | 45.0% |
| CGGTTGAGAAAATGGAAGAC+AGG | + | chr3_4:69512596-69512615 | None:intergenic | 45.0% | |
| GTATTTGCACCTTGTTGCAG+TGG | - | chr3_4:69512759-69512778 | Msa0517790:intron | 45.0% | |
| ! | GTAACAAGTCTGAGACCCAC+GGG | + | chr3_4:69512275-69512294 | None:intergenic | 50.0% |
| ! | GGTAACAAGTCTGAGACCCA+CGG | + | chr3_4:69512276-69512295 | None:intergenic | 50.0% |
| ! | GTAACAAGTCTGAGACCCAC+GGG | + | chr3_4:69512275-69512294 | None:intergenic | 50.0% |
| ! | GGTAACAAGTCTGAGACCCA+CGG | + | chr3_4:69512276-69512295 | None:intergenic | 50.0% |
| CAGGCAGACATGTGGAGTGT+CGG | - | chr3_4:69512405-69512424 | Msa0517790:intron | 55.0% | |
| ! | AGGCAGACATGTGGAGTGTC+GGG | - | chr3_4:69512406-69512425 | Msa0517790:intron | 55.0% |
| ATAGGCGCACCACTGCAACA+AGG | + | chr3_4:69512771-69512790 | None:intergenic | 55.0% | |
| CAGGCAGACATGTGGAGTGT+CGG | - | chr3_4:69512405-69512424 | Msa0517790:intron | 55.0% | |
| ! | AGGCAGACATGTGGAGTGTC+GGG | - | chr3_4:69512406-69512425 | Msa0517790:intron | 55.0% |
| ATAGGCGCACCACTGCAACA+AGG | + | chr3_4:69512771-69512790 | None:intergenic | 55.0% | |
| !! | GGCAGACATGTGGAGTGTCG+GGG | - | chr3_4:69512407-69512426 | Msa0517790:intron | 60.0% |
| !! | GGCAGACATGTGGAGTGTCG+GGG | - | chr3_4:69512407-69512426 | Msa0517790:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 69511975 | 69512938 | 69511975 | ID=Msa0517790;Name=Msa0517790 |
| chr3_4 | mRNA | 69511975 | 69512938 | 69511975 | ID=Msa0517790-mRNA-1;Parent=Msa0517790;Name=Msa0517790-mRNA-1;_AED=0.47;_eAED=0.92;_QI=0|0|0|1|1|1|2|0|54 |
| chr3_4 | exon | 69512897 | 69512938 | 69512897 | ID=Msa0517790-mRNA-1:exon:18554;Parent=Msa0517790-mRNA-1 |
| chr3_4 | exon | 69511975 | 69512097 | 69511975 | ID=Msa0517790-mRNA-1:exon:18553;Parent=Msa0517790-mRNA-1 |
| chr3_4 | CDS | 69512897 | 69512938 | 69512897 | ID=Msa0517790-mRNA-1:cds;Parent=Msa0517790-mRNA-1 |
| chr3_4 | CDS | 69511975 | 69512097 | 69511975 | ID=Msa0517790-mRNA-1:cds;Parent=Msa0517790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
