AlfalfaGEDB | Msa0518020

M. sativa cultivar zhongmu-4 / Msa0518020

KEGG: -
GO: -
PFAM: PF00642.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518020	XP_003602587.2	97.259	985	26	1	1	985	1	984	0.0	1994

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518020	sp\|Q9LIH5\|C3H38_ARATH	34.272	426	181	15	596	985	305	667	2.93e-40	163

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518020	G7JBH8	97.259	985	26	1	1	985	1	984	0.0	1994

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TFs/TRs:

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Gene ID	Type	Classification
Msa0518020	TF	C3H

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0378060	Msa0518020	0.921890	2.000375e-88	-8.615850e-47
Msa0470200	Msa0518020	0.828854	7.133430e-55	-8.615850e-47
Msa0518020	Msa0621140	0.894703	1.921248e-75	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518020	MtrunA17_Chr3g0129941	94.822	985	27	2	1	985	1	961	0.0	1932
Msa0518020	MtrunA17_Chr8g0384131	47.353	699	304	16	292	985	131	770	4.53e-177	541
Msa0518020	MtrunA17_Chr8g0384131	53.150	254	91	6	1	244	1	236	1.60e-57	214
Msa0518020	MtrunA17_Chr3g0130701	96.400	250	9	0	982	1231	84	333	2.37e-171	509
Msa0518020	MtrunA17_Chr8g0384701	69.796	245	70	1	985	1229	16	256	3.09e-117	365
Msa0518020	MtrunA17_Chr1g0202951	36.771	223	130	5	983	1204	31	243	3.56e-40	149
Msa0518020	MtrunA17_Chr2g0308451	45.745	94	51	0	891	984	1345	1438	9.98e-19	92.8
Msa0518020	MtrunA17_Chr2g0282581	44.578	83	46	0	903	985	800	882	2.54e-14	78.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518020	AT1G27990.1	72.685	216	56	1	1000	1215	44	256	2.61e-105	333
Msa0518020	AT3G18640.3	34.272	426	181	15	596	985	305	667	2.98e-41	163
Msa0518020	AT3G18640.1	34.272	426	181	15	596	985	305	667	2.98e-41	163
Msa0518020	AT3G18640.2	34.272	426	181	15	596	985	305	667	2.98e-41	163
Msa0518020	AT5G52420.1	35.678	199	122	3	1003	1201	44	236	4.13e-35	135
Msa0518020	AT2G10553.1	75.000	72	18	0	914	985	2	73	3.94e-30	114
Msa0518020	AT5G23920.1	33.649	211	114	6	1000	1201	29	222	1.23e-25	107
Msa0518020	AT3G26850.2	48.193	83	40	1	905	984	173	255	2.84e-17	84.0
Msa0518020	AT3G26850.1	48.193	83	40	1	905	984	173	255	2.84e-17	84.0
Msa0518020	AT2G33835.1	44.156	77	42	1	910	985	502	578	5.64e-13	73.9

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Find 424 sgRNAs with CRISPR-Local

Find 892 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCCCCGCCGACATCT+AGG	0.344379	3_4:+69814637	Msa0518020:five_prime_UTR
AAAATGGAACATGGAAAGCA+GGG	0.638602	3_4:+69817343	Msa0518020:five_prime_UTR
AAACTATTGGAAAATATTGA+AGG	0.501878	3_4:-69815564	None:intergenic
AAACTCCCCGCCGACATCTA+GGG	0.558366	3_4:+69814638	Msa0518020:five_prime_UTR
AAACTTGGGTAATTCAATTA+AGG	0.210347	3_4:+69823262	Msa0518020:CDS
AAAGACAGCAAGAAAAGATG+CGG	0.680730	3_4:-69824360	None:intergenic
AAAGCTCTTGGAAGGAACCT+TGG	0.549636	3_4:-69825154	None:intergenic
AAAGCTTAACAAACTTGTAC+AGG	0.519903	3_4:+69823691	Msa0518020:CDS
AAAGGCTAAGAGATGATAGG+TGG	0.588164	3_4:+69822064	Msa0518020:CDS
AAAGTTGTTGATAAAGTAAC+GGG	0.454132	3_4:+69823599	Msa0518020:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAAACAAAGATCTACGA+TGG	-	chr3_4:69814584-69814603	None:intergenic	30.0%
ACCCTAGATAAGATATAGTA+AGG	-	chr3_4:69814716-69814735	None:intergenic	30.0%
CAAGGTGAAGTTGAAATTAA+GGG	-	chr3_4:69814750-69814769	None:intergenic	30.0%
ACAAGGTGAAGTTGAAATTA+AGG	-	chr3_4:69814751-69814770	None:intergenic	30.0%
TCGATGAAACGAAACTTATT+TGG	+	chr3_4:69815127-69815146	Msa0518020:intron	30.0%
ATCAAAAGCCTAAAGAATGT+GGG	-	chr3_4:69815229-69815248	None:intergenic	30.0%
TACAAAGAACTAAAGCACAT+AGG	-	chr3_4:69815335-69815354	None:intergenic	30.0%
TTGTATCTCATATTAGCACT+TGG	-	chr3_4:69815379-69815398	None:intergenic	30.0%
TGTGCATCAAAGAAAAGAAT+TGG	+	chr3_4:69815702-69815721	Msa0518020:five_prime_UTR	30.0%
GATCTATATTTGTGATGTGA+TGG	-	chr3_4:69815849-69815868	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	69814495	69825282	69814495	ID=Msa0518020;Name=Msa0518020
chr3_4	mRNA	69814495	69825282	69814495	ID=Msa0518020-mRNA-1;Parent=Msa0518020;Name=Msa0518020-mRNA-1;_AED=0.06;_eAED=0.06;_QI=2211\|0.66\|0.71\|1\|0.66\|0.57\|7\|0\|1231
chr3_4	exon	69814495	69814696	69814495	ID=Msa0518020-mRNA-1:exon:18673;Parent=Msa0518020-mRNA-1
chr3_4	exon	69815410	69817379	69815410	ID=Msa0518020-mRNA-1:exon:18674;Parent=Msa0518020-mRNA-1
chr3_4	exon	69818180	69818553	69818180	ID=Msa0518020-mRNA-1:exon:18675;Parent=Msa0518020-mRNA-1
chr3_4	exon	69820934	69822534	69820934	ID=Msa0518020-mRNA-1:exon:18676;Parent=Msa0518020-mRNA-1
chr3_4	exon	69822694	69823712	69822694	ID=Msa0518020-mRNA-1:exon:18677;Parent=Msa0518020-mRNA-1
chr3_4	exon	69824018	69824545	69824018	ID=Msa0518020-mRNA-1:exon:18678;Parent=Msa0518020-mRNA-1
chr3_4	exon	69825070	69825282	69825070	ID=Msa0518020-mRNA-1:exon:18679;Parent=Msa0518020-mRNA-1
chr3_4	five_prime_UTR	69814495	69814696	69814495	ID=Msa0518020-mRNA-1:five_prime_utr;Parent=Msa0518020-mRNA-1
chr3_4	five_prime_UTR	69815410	69817379	69815410	ID=Msa0518020-mRNA-1:five_prime_utr;Parent=Msa0518020-mRNA-1
chr3_4	five_prime_UTR	69818180	69818218	69818180	ID=Msa0518020-mRNA-1:five_prime_utr;Parent=Msa0518020-mRNA-1
chr3_4	CDS	69818219	69818553	69818219	ID=Msa0518020-mRNA-1:cds;Parent=Msa0518020-mRNA-1
chr3_4	CDS	69820934	69822534	69820934	ID=Msa0518020-mRNA-1:cds;Parent=Msa0518020-mRNA-1
chr3_4	CDS	69822694	69823712	69822694	ID=Msa0518020-mRNA-1:cds;Parent=Msa0518020-mRNA-1
chr3_4	CDS	69824018	69824545	69824018	ID=Msa0518020-mRNA-1:cds;Parent=Msa0518020-mRNA-1
chr3_4	CDS	69825070	69825282	69825070	ID=Msa0518020-mRNA-1:cds;Parent=Msa0518020-mRNA-1

Gene Sequence

>Msa0518020

ATGAGTGGAGCTGGCAGAAAGCACTCTTCAAAGTGGGATTTGAGTGATGAGCATAAATTCTCACCTGGTAGCAAGCAAATGCGGTCTGGACGGTCCTCTGCAGATGTTGCTGGTAGCAATAGTTCAGAATGGGCTTATTTGGAAGGAAATGATAAATTAAGACCTGTTACGGGATTTTCATCAAAGGAATCTTATTATGGAGGCAGAGGTTCAAATGAGAACGATGCTATGAATAAAGACCATAGAGTTTTTAATTCAAGAAGAGAATGGGACACTGATGGAAGTTACAGCAGGAACATGAGACACAGTCAGTCCCCTAAAAATGACTGGAGCAGGAACAGTCGGAGCCGGAGTCGGAGCCCTCCTCGTGGTTTCAGGTGGGATTCAGGAGTTGATGGTAGAAAAAGGACGAGGGTTGGAGGATCAACACGACCGTGCAGAGATTTTGCTGTTGGAAAATGTAGAAGAGGTAGTCATTGCAATTTTCTGCATCATGATAACCAAAATCGTGAGAATAGTTGGGAAGGTAGGCACAGGGAAGATGGAGCTCCCAGATACTCTGCTACCCATGAGAGTGTTGACCATTCTCTTAAGAGTGGTAGATCTAATGAAGCTTGTATAAATTTTGCAAAAGGCAGCTGTAGAATGGGAGCATCTTGCAAGTTTGTGCATGATTACGATTCTGATGGATATGGTAAAGTTTCTATGGATGAGTTCACTAGAGAAAGGGAAGATGAAGCTCTCAGATATCCTGCTACCCATGAGAGCAGGGACCATTCTCTTAAGAGTGGTAGATCTAATGAATTCATTAGAGAAAGAGAAGATGGAGCTCGCAGATATCCTGCTACCCATGAGAGCAGGGACCATTCTCTTAAGAGTGGTAGATCTAATGAATTCACTAGAGAAAGGGAAAATGGAGGTCCCAGATATTCTGCTACCTATGAGAGCAGGGACCATTCTCCAAAGAGTGGTAGATCTAATGAATTCACTAGTGAAAGGGAAGATGGAGCTCCCAGATATCCTACTACCCATGAGAGCAGGGACTATTCTCTTAAGAGTGGTAGATCTAATGAATTCACTAGTGAAAGGGAAGATGGAGCTCCCAGATATCCTACTAACCACGAGAGCAGGGACTATTCTTTTAAGGGCGGTAGATCTAATGACGAATATGGCAAAGTTTTTATGGATGAATTCACTAGAGAAAGAGAAGTTGGCAGAAGGCACAGAGATAATTCTTTTCAGCAGGGTGGTGGCCGGCATGTGCCTAACCGCACTATTGATGCTCCTTGCAAATTTTTTGCTAGTGGAAATTGTCGAAATGGTAAACATTGTAGGTTTTCTCACGATACGCAAGCATGTAGGAGCCCTATTAGAAGATTAAGGGATGATAGATGGACAAGAAATCCAAGTAGAGATCATCAAATGTTGGATAGACGAAAATTGAGTGACTCCATTAGTCCAAATAAAAGGCTAAGAGATGATAGGTGGGGTTCAGATAGTGACATGGCTGATCCTGATAGAGTCGAGGACAGTCCAAAGCAGAATGATACAGTTTCTGGCTCTGATACAGCAAAGCTGATCGAGAATAAAAGTGGAAATGTAGGTACCACAGAGCCAGAATTTACTGATTTGCCTATAACGGATGGATGGGGGCATGGTTTAGATAAGAGCGGGCTGCATGCTAAACCACCAATTTTGAGTGATAAGAAGGAAGCTGATCTTTGGATAGCAGAAAATACTGGTGCCAACATGCATGGTTCCCAGTCAATAGGTACAACGGACATTTGGCCGGGTGATGCAGAAATGTCTCCTGATTGGAATTATAGGATGGGGTCTTCCAGCCATATGGAAGAACATAAACAGAAGGAACATGATGTAAGCCAGGGTGGTACATATCTAGCCATCTCTGAACACAACAGAATACAACTTGCTCCAGGACAAAACATCAATCAGAATACAGAGAATGTAAATCCTTTGCATACTTCCAGCTGCCATGCAGTTGGACAAAGTCAAGTGGATGTTCCCATTCTTTCTTCAAGAGAAGGCATTGTTGATGCTATACACAGCCAGGAAGTATCTACTGAGCAAAAGTACACCGGAGAACCAAGTATTATGGATTCAGGCTTATCACAAGTTAGTTCAATAAATCCTCCAGTTCAAAACGTAGTAAGCAATGAACAGCTTGCTCAACTCACGAATCTCTCAGCCTCTCTGGCTCATATCCTTGGAGCAGGTCAGCAGCTGCCGCAGCTATATGCTGCTTTAAATTCTCATGAATTGAAGGACAGTCCTTCCCAAGCAAAAATTCAAGTGCCTGCAATGCCTATTTCAATCACATGCATCAAGCCAGATCCTGCCGTTGAACTCCCAAAACAGTATGATCCAATGAATGATAGTATTGAGCAAAAGAATGCTGATGCAAGTGGCGTACCCCCAGCCATTCCTCCAAGTAAAACTATTGCCGAAGTAGAAATTCTGTCGCAGTTGTCTAACCCAGGAAGAGAAAACTTGGGTAATTCAATTAAGGGTGCCTCCTCAGAACATGTCAAGAGTGATAATCTAATCCATTTGCAGCCTGGTCAAAACACGGTGGTCAATAAGGATAACAATGAAGAGGTAGCTAAGGAAAGGAAAAATTCTCAAGATGGTCATAAAAGTACCAAAGAGAATGGTCCTCAAAACATGGACCAAAATGCTGGACCTGATGATGCCAAGCAAACAAAGGAAATGAAAGGATCACGTGCATTTAAATTTGCATTGGCTGAGTTTGTTAAGGAGCTTCTGAAACCAACTTGGAAAGAAGGTAAAATCACTAAAGAAGATTATAAAACGATTGTGAAGAAAGTTGTTGATAAAGTAACGGGTACCATCCAGGAGGCCAATATTCCGCAGACACAAGAAAAAATTGACCAATATTTGTCATTTTCAAAACCAAAGCTTAACAAACTTGTACAGCGAACAGAAGCACCTTCGGCAGCTGGAATGATGGGTTCACTACGTGTAATAGAGCTTCAACTTGTTGCATTCATATTAGTTTTCTCAGCAAGTGGTCTTGTTCCACTTCTTGATCTAGTTTTTCCAGCTCTTGCCTCTGCATACATCTTAGCACTTGCACGTTACGCATTTCCATCATCTCCTTCTACTACATCAAATTCACAACAACAAGAGATCTTCAAAGGAAGTAGCATGTTCAGAATGTATGTTATTGTTGGAACAACTATTGGGTTGTTTTTGCCACTGGCTTATGTTCTTGGAGGGTTTGCAAGAGGTGATCAACATGCTGTGCGTTCTGCAACACCGCATCTTTTCTTGCTGTCTTTTCAGATATTGACTGAGAATATTATTAGCAGTTTGTCTCTATTTTCACCACCTGTTAGAGCTTTGGTTCCTATGATTTATACTATTAGAAGGATCTTTGTTGATATTGATTGGATCAATGATGTTTGGCTCAACAAGACTTTGCCTGCCAATGCACGCTTTCAGGACACAGCATGGTATTGGTTTGGGAAGGGTCTAGCCGTGGCGAATTTGGCTTATTTCTCAATCAATCTATGTGTATTCTTGATACCAAGGTTCCTTCCAAGAGCTTTTGAGAGGTATTTCCAAGAAAGAGGAGAGATTTATGCCAAGAGTGCAGAAGATAAGCGATCTATGTCTCTCAACAAACCCCAATTATCAGAGAAGAAGATGGATTAA

Protein sequence

>Msa0518020

MSGAGRKHSSKWDLSDEHKFSPGSKQMRSGRSSADVAGSNSSEWAYLEGNDKLRPVTGFSSKESYYGGRGSNENDAMNKDHRVFNSRREWDTDGSYSRNMRHSQSPKNDWSRNSRSRSRSPPRGFRWDSGVDGRKRTRVGGSTRPCRDFAVGKCRRGSHCNFLHHDNQNRENSWEGRHREDGAPRYSATHESVDHSLKSGRSNEACINFAKGSCRMGASCKFVHDYDSDGYGKVSMDEFTREREDEALRYPATHESRDHSLKSGRSNEFIREREDGARRYPATHESRDHSLKSGRSNEFTRERENGGPRYSATYESRDHSPKSGRSNEFTSEREDGAPRYPTTHESRDYSLKSGRSNEFTSEREDGAPRYPTNHESRDYSFKGGRSNDEYGKVFMDEFTREREVGRRHRDNSFQQGGGRHVPNRTIDAPCKFFASGNCRNGKHCRFSHDTQACRSPIRRLRDDRWTRNPSRDHQMLDRRKLSDSISPNKRLRDDRWGSDSDMADPDRVEDSPKQNDTVSGSDTAKLIENKSGNVGTTEPEFTDLPITDGWGHGLDKSGLHAKPPILSDKKEADLWIAENTGANMHGSQSIGTTDIWPGDAEMSPDWNYRMGSSSHMEEHKQKEHDVSQGGTYLAISEHNRIQLAPGQNINQNTENVNPLHTSSCHAVGQSQVDVPILSSREGIVDAIHSQEVSTEQKYTGEPSIMDSGLSQVSSINPPVQNVVSNEQLAQLTNLSASLAHILGAGQQLPQLYAALNSHELKDSPSQAKIQVPAMPISITCIKPDPAVELPKQYDPMNDSIEQKNADASGVPPAIPPSKTIAEVEILSQLSNPGRENLGNSIKGASSEHVKSDNLIHLQPGQNTVVNKDNNEEVAKERKNSQDGHKSTKENGPQNMDQNAGPDDAKQTKEMKGSRAFKFALAEFVKELLKPTWKEGKITKEDYKTIVKKVVDKVTGTIQEANIPQTQEKIDQYLSFSKPKLNKLVQRTEAPSAAGMMGSLRVIELQLVAFILVFSASGLVPLLDLVFPALASAYILALARYAFPSSPSTTSNSQQQEIFKGSSMFRMYVIVGTTIGLFLPLAYVLGGFARGDQHAVRSATPHLFLLSFQILTENIISSLSLFSPPVRALVPMIYTIRRIFVDIDWINDVWLNKTLPANARFQDTAWYWFGKGLAVANLAYFSINLCVFLIPRFLPRAFERYFQERGEIYAKSAEDKRSMSLNKPQLSEKKMD*