AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0521930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0521930 MtrunA17_Chr3g0140331 92.615 325 14 3 1 322 47 364 0.0 546
Msa0521930 MtrunA17_Chr1g0146231 53.429 350 117 10 6 322 8 344 1.51e-108 320
Msa0521930 MtrunA17_Chr8g0340401 44.658 365 136 13 1 322 1 342 2.95e-80 247
Msa0521930 MtrunA17_Chr8g0372291 40.000 285 121 10 19 281 32 288 8.96e-42 146
Msa0521930 MtrunA17_Chr4g0035891 36.824 296 143 12 1 280 13 280 1.42e-41 146
Msa0521930 MtrunA17_Chr3g0145121 39.777 269 144 10 19 281 29 285 1.48e-38 138
Msa0521930 MtrunA17_Chr1g0199671 36.502 263 106 8 69 275 49 306 2.12e-36 133
Msa0521930 MtrunA17_Chr6g0461591 37.333 225 90 6 103 277 35 258 4.07e-35 128
Msa0521930 MtrunA17_Chr7g0253101 41.038 212 82 7 103 275 45 252 1.21e-34 127
Msa0521930 MtrunA17_Chr5g0404981 40.265 226 97 7 89 281 150 370 2.07e-34 129
Msa0521930 MtrunA17_Chr1g0169431 78.873 71 15 0 210 280 171 241 1.43e-33 124
Msa0521930 MtrunA17_Chr5g0395391 76.056 71 17 0 210 280 269 339 1.75e-32 123
Msa0521930 MtrunA17_Chr5g0395391 51.200 125 48 3 150 274 120 231 1.56e-29 115
Msa0521930 MtrunA17_Chr1g0213161 60.674 89 28 2 208 293 149 233 6.56e-30 114
Msa0521930 MtrunA17_Chr4g0028961 62.963 81 30 0 211 291 232 312 6.86e-30 115
Msa0521930 MtrunA17_Chr2g0326771 75.385 65 16 0 211 275 216 280 2.34e-29 114
Msa0521930 MtrunA17_Chr7g0232671 56.962 79 31 2 200 277 175 251 4.73e-23 95.9
Msa0521930 MtrunA17_Chr7g0232681 36.413 184 99 8 90 271 25 192 2.89e-21 89.7
Msa0521930 MtrunA17_Chr7g0232701 53.947 76 31 1 196 271 62 133 1.54e-20 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0521930 AT4G32890.1 47.484 318 117 9 30 322 16 308 3.81e-85 259
Msa0521930 AT5G25830.1 48.023 354 125 12 2 322 4 331 4.40e-85 259
Msa0521930 AT3G60530.1 48.039 204 74 5 88 291 63 234 1.34e-51 171
Msa0521930 AT2G45050.1 44.697 264 105 10 40 288 20 257 1.29e-49 166
Msa0521930 AT4G36240.1 40.234 256 109 7 32 278 11 231 5.64e-39 138
Msa0521930 AT5G66320.2 40.000 245 90 6 89 291 100 329 2.92e-36 133
Msa0521930 AT5G66320.1 40.000 245 90 6 89 291 100 329 2.92e-36 133
Msa0521930 AT3G24050.1 77.941 68 15 0 210 277 193 260 4.51e-33 123
Msa0521930 AT1G08010.2 67.073 82 21 1 208 283 217 298 4.45e-32 121
Msa0521930 AT1G08010.1 67.073 82 21 1 208 283 217 298 4.45e-32 121
Msa0521930 AT1G08010.3 67.073 82 21 1 208 283 217 298 4.45e-32 121
Msa0521930 AT3G54810.1 75.000 68 17 0 211 278 229 296 9.19e-32 121
Msa0521930 AT3G54810.2 75.000 68 17 0 211 278 229 296 9.19e-32 121
Msa0521930 AT4G34680.1 43.478 184 73 7 98 280 96 249 3.67e-31 118
Msa0521930 AT4G34680.2 43.478 184 73 7 98 280 96 249 3.67e-31 118
Msa0521930 AT3G51080.1 73.913 69 18 0 211 279 221 289 4.21e-30 116
Msa0521930 AT1G08000.2 60.227 88 30 1 208 290 215 302 4.23e-30 116
Msa0521930 AT1G08000.1 60.227 88 30 1 208 290 215 302 4.23e-30 116
Msa0521930 AT3G45170.1 62.319 69 26 0 211 279 115 183 6.37e-26 102
Msa0521930 AT2G28340.1 68.254 63 20 0 213 275 193 255 1.22e-24 101

Find 104 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGATTCCTCAGCAAATT+TGG 0.139979 3_4:+74954446 None:intergenic
CACGCATTGCATAGTGTCTT+TGG 0.211564 3_4:+74954075 None:intergenic
CTACATGAGGTGCGTGAAAT+CGG 0.267854 3_4:+74953819 None:intergenic
ACAGTTGATATCTGGATTAA+AGG 0.306992 3_4:-74954400 Msa0521930:CDS
CATAAGCTACAGTTGATATC+TGG 0.309428 3_4:-74954408 Msa0521930:CDS
AAGGGCCGTCGGCAGAATTC+TGG 0.309787 3_4:+74956028 None:intergenic
CATAGTGTCTTTGGGCCTAA+TGG 0.325824 3_4:+74954084 None:intergenic
CAGTGGCGCACCGGGCCATT+AGG 0.349868 3_4:-74954099 Msa0521930:CDS
GTGAGAGAGAAAAGCATAAG+AGG 0.370517 3_4:+74956417 None:intergenic
CACGTGTGCCTCCATGCAAC+TGG 0.370780 3_4:-74954284 Msa0521930:CDS
GGGCCCTCATCATCTCCTTC+TGG 0.374473 3_4:+74953938 None:intergenic
ATTGTGGACAGAAGCTGGTT+TGG 0.397002 3_4:+74956247 None:intergenic
ACGCATTGCATAGTGTCTTT+GGG 0.408202 3_4:+74954076 None:intergenic
TTGCAACAATTGGTGTTGTT+GGG 0.408578 3_4:+74953918 None:intergenic
GTTGCAACAATTGGTGTTGT+TGG 0.409369 3_4:+74953917 None:intergenic
GACACTTGAGGGTGTATTGT+TGG 0.425894 3_4:+74954336 None:intergenic
TGCTTAGTCAACACAAATGT+TGG 0.431309 3_4:+74953997 None:intergenic
CATCACAGCATTATGTTTGA+AGG 0.433831 3_4:-74953886 Msa0521930:CDS
ACAAAGTGTGACAAAGTTTG+TGG 0.433866 3_4:+74956385 None:intergenic
TCTCTTTGATTTCTCTAATG+AGG 0.434233 3_4:-74956160 Msa0521930:CDS
CGGCAAAAGCCCGTAGTAAA+CGG 0.443499 3_4:-74954308 Msa0521930:CDS
TTCGAAAGTGGGGTCTTCAA+TGG 0.445894 3_4:+74956131 None:intergenic
TACATGAGGTGCGTGAAATC+GGG 0.448275 3_4:+74953820 None:intergenic
TTGCACCTTTCGATGTGAGT+TGG 0.456005 3_4:+74953972 None:intergenic
CTCCGGCGAACTCTCCGTTC+CGG 0.462198 3_4:-74956004 Msa0521930:intron
ACGGAGAGTTCGCCGGAGAA+AGG 0.464710 3_4:+74956009 None:intergenic
GTGGTGGTGGTGGTCGTGGT+TGG 0.465201 3_4:+74954237 None:intergenic
GTTTGTGGTGGAATTGTTGT+CGG 0.468374 3_4:+74956224 None:intergenic
TTGCCGGGACAGACACTTGA+GGG 0.471708 3_4:+74954325 None:intergenic
ACAAATGTTGGGCTTGCTGC+AGG 0.475199 3_4:+74954009 None:intergenic
ATGATCTTGCGCAGCTAGAA+TGG 0.476849 3_4:-74954473 Msa0521930:CDS
CCTCATGTAGTACGGTGCTT+TGG 0.478377 3_4:-74953806 Msa0521930:exon
GTGTGACAAAGTTTGTGGTT+TGG 0.486157 3_4:+74956390 None:intergenic
TCATCATCTCCTTCTGGCGT+CGG 0.486176 3_4:+74953944 None:intergenic
CGGAGAGTTCGCCGGAGAAA+GGG 0.487872 3_4:+74956010 None:intergenic
AGATGTGGTGGTGGTGGTCG+TGG 0.490069 3_4:+74954233 None:intergenic
ATTAATATTCAAAGCTGCGT+TGG 0.490776 3_4:+74953768 None:intergenic
CTCTTGAGCTTCCATGGGTT+AGG 0.491426 3_4:+74956275 None:intergenic
AATATTCAAAGCTGCGTTGG+AGG 0.492539 3_4:+74953771 None:intergenic
GCTTAGTCAACACAAATGTT+GGG 0.498811 3_4:+74953998 None:intergenic
AGCAGTGTTTACATTGTTTG+TGG 0.499828 3_4:+74956209 None:intergenic
TCTGCCGACGGCCCTTTCTC+CGG 0.501462 3_4:-74956021 Msa0521930:CDS
AGAATTCTGGCACGAATTGT+CGG 0.501620 3_4:+74956041 None:intergenic
GTGGAATTGTTGTCGGATTG+TGG 0.509048 3_4:+74956231 None:intergenic
AGGAGGAGATTCTTCGAAAG+TGG 0.514544 3_4:+74956119 None:intergenic
GATGTTGTAGTTGCAACAAT+TGG 0.517809 3_4:+74953908 None:intergenic
ACACTTGAGGGTGTATTGTT+GGG 0.518688 3_4:+74954337 None:intergenic
AGACTCCACAGTGGCGCACC+GGG 0.518894 3_4:-74954107 Msa0521930:CDS
AAGACTCCACAGTGGCGCAC+CGG 0.525177 3_4:-74954108 Msa0521930:CDS
TTGAGCTTCCATGGGTTAGG+AGG 0.527789 3_4:+74956278 None:intergenic
TGTCTTTGGGCCTAATGGCC+CGG 0.529224 3_4:+74954089 None:intergenic
GAATCATTAGAGTTGGTAGG+AGG 0.531077 3_4:+74956102 None:intergenic
GCAAGAGCTCCGACGCCAGA+AGG 0.533988 3_4:-74953953 Msa0521930:CDS
GTCGGATTGTGGACAGAAGC+TGG 0.535564 3_4:+74956242 None:intergenic
GACCTCGGTGATGGAGGAAA+GGG 0.539815 3_4:-74954159 Msa0521930:CDS
GGAGAATCATTAGAGTTGGT+AGG 0.541668 3_4:+74956099 None:intergenic
ATATCAACTGTAGCTTATGA+AGG 0.545098 3_4:+74954412 None:intergenic
AGGAGGAGAATCATTAGAGT+TGG 0.548174 3_4:+74956095 None:intergenic
AGACCTCGGTGATGGAGGAA+AGG 0.549246 3_4:-74954160 Msa0521930:CDS
ATAATGAAGTGAGAAAGTAT+AGG 0.561648 3_4:+74956348 None:intergenic
AATGGCCCGGTGCGCCACTG+TGG 0.562645 3_4:+74954102 None:intergenic
GTCGACTCGTACCTGAGTAT+AGG 0.562857 3_4:-74954032 Msa0521930:CDS
CACACGTGACCGTTTACTAC+GGG 0.563431 3_4:+74954299 None:intergenic
GGAGGAGATTCTTCGAAAGT+GGG 0.565310 3_4:+74956120 None:intergenic
TGTGGAGTCAGATACAAGTC+AGG 0.567755 3_4:-74954054 Msa0521930:CDS
TACCGGAACGGAGAGTTCGC+CGG 0.569597 3_4:+74956002 None:intergenic
GACGCCAGAAGGAGATGATG+AGG 0.570929 3_4:-74953942 Msa0521930:CDS
AAAAGAGACCTCGGTGATGG+AGG 0.575559 3_4:-74954165 Msa0521930:CDS
GTGAGAAGATGTGGTGGTGG+TGG 0.576607 3_4:+74954227 None:intergenic
GAAGTGGGATTGATTTCAAG+AGG 0.577002 3_4:+74956075 None:intergenic
AGAGATCTTCAACGATGAAG+TGG 0.579783 3_4:+74956178 None:intergenic
GCACACGTGACCGTTTACTA+CGG 0.581455 3_4:+74954298 None:intergenic
AGGCGCGAAGTCCAGTTGCA+TGG 0.582876 3_4:+74954273 None:intergenic
TTTGCCGGGACAGACACTTG+AGG 0.592935 3_4:+74954324 None:intergenic
ACACCCTCAAGTGTCTGTCC+CGG 0.593472 3_4:-74954328 Msa0521930:CDS
AATCAAGTAATCATCACCGT+TGG 0.595692 3_4:+74953858 None:intergenic
AGAGTGAGAAGATGTGGTGG+TGG 0.596239 3_4:+74954224 None:intergenic
GTGGGATTGATTTCAAGAGG+AGG 0.599204 3_4:+74956078 None:intergenic
AAGTAATCATCACCGTTGGA+TGG 0.601160 3_4:+74953862 None:intergenic
TATAATGGCACGTACCGGAA+CGG 0.606350 3_4:+74955990 None:intergenic
ACGCCAGAAGGAGATGATGA+GGG 0.609091 3_4:-74953941 Msa0521930:CDS
CAACAACACCTCCTAACCCA+TGG 0.609995 3_4:-74956286 Msa0521930:exon
TTCGCCGGAGAAAGGGCCGT+CGG 0.611125 3_4:+74956017 None:intergenic
GCACTCCAACTCACATCGAA+AGG 0.612333 3_4:-74953977 Msa0521930:CDS
TTCACGCACCTCATGTAGTA+CGG 0.615224 3_4:-74953814 Msa0521930:exon
AGTGTTTACATTGTTTGTGG+TGG 0.618078 3_4:+74956212 None:intergenic
CTTGCTGCAGGCCTATACTC+AGG 0.624212 3_4:+74954021 None:intergenic
ATCTTCAACGATGAAGTGGT+CGG 0.624703 3_4:+74956182 None:intergenic
ATCTTCTCACTCTGACACCA+TGG 0.629511 3_4:-74954214 Msa0521930:CDS
TCGTGCCAGAATTCTGCCGA+CGG 0.642132 3_4:-74956033 Msa0521930:CDS
GTCAGAGTGAGAAGATGTGG+TGG 0.644152 3_4:+74954221 None:intergenic
ATGTTTGAAGGACCATCCAA+CGG 0.648434 3_4:-74953874 Msa0521930:CDS
CCAAAGCACCGTACTACATG+AGG 0.651273 3_4:+74953806 None:intergenic
CCACTGTGGAGTCTTATCAG+TGG 0.652363 3_4:+74954116 None:intergenic
ATGGATGTCCAAATTTGCTG+AGG 0.653086 3_4:-74954454 Msa0521930:CDS
TGGTTGGAGAGAGAACTAGA+AGG 0.657111 3_4:+74954253 None:intergenic
CGCGAAGTCCAGTTGCATGG+AGG 0.662375 3_4:+74954276 None:intergenic
TACTTGATTCATCAACACGT+GGG 0.667702 3_4:-74953844 Msa0521930:CDS
GGTGTCAGAGTGAGAAGATG+TGG 0.671117 3_4:+74954218 None:intergenic
AAGACACTATGCAATGCGTG+TGG 0.676419 3_4:-74954072 Msa0521930:CDS
TTACTTGATTCATCAACACG+TGG 0.680358 3_4:-74953845 Msa0521930:CDS
GAGGAGATTCTTCGAAAGTG+GGG 0.692052 3_4:+74956121 None:intergenic
GACCCTTTCCTCCATCACCG+AGG 0.692792 3_4:+74954157 None:intergenic
CCACTGATAAGACTCCACAG+TGG 0.837860 3_4:-74954116 Msa0521930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTTATATACAACAAT+TGG - chr3_4:74954155-74954174 Msa0521930:CDS 10.0%
!! CTATAAATATATGATAAAAA+AGG + chr3_4:74954673-74954692 None:intergenic 10.0%
!! TAGTTAAAAAAAAAATATAG+GGG + chr3_4:74955005-74955024 None:intergenic 10.0%
!!! AAATAAAGAGAGTATTATTT+AGG + chr3_4:74954232-74954251 None:intergenic 15.0%
!! ATGTGACAAAATTAAAAATA+GGG + chr3_4:74954619-74954638 None:intergenic 15.0%
!! TATGTGACAAAATTAAAAAT+AGG + chr3_4:74954620-74954639 None:intergenic 15.0%
!! AGTTAAAAAAAAAATATAGG+GGG + chr3_4:74955004-74955023 None:intergenic 15.0%
!! ATATTTGTAATAAAAGCTAA+GGG + chr3_4:74955054-74955073 None:intergenic 15.0%
!! TATATTTGTAATAAAAGCTA+AGG + chr3_4:74955055-74955074 None:intergenic 15.0%
!!! TATTATTTTTGTTTAGAGAA+GGG + chr3_4:74955093-74955112 None:intergenic 15.0%
!!! TTATTATTTTTGTTTAGAGA+AGG + chr3_4:74955094-74955113 None:intergenic 15.0%
!!! TCTTAAGATATACTTTTTTT+AGG + chr3_4:74955244-74955263 None:intergenic 15.0%
!!! TCATTTTTAAGATACTATAT+CGG + chr3_4:74955284-74955303 None:intergenic 15.0%
!! ATATAAAGTAAGAATTCTAA+TGG - chr3_4:74955311-74955330 Msa0521930:intron 15.0%
!! TTTCATAAGTATGACAATTA+TGG + chr3_4:74954421-74954440 None:intergenic 20.0%
!! TCTTAAGACAACAATTAAAA+AGG - chr3_4:74955256-74955275 Msa0521930:intron 20.0%
!!! ATTAGGTTATGTATTTTCTA+AGG + chr3_4:74955462-74955481 None:intergenic 20.0%
!! AGAAAATACATAACCTAATA+TGG - chr3_4:74955463-74955482 Msa0521930:intron 20.0%
!! ATAATGAAGTGAGAAAGTAT+AGG + chr3_4:74953704-74953723 None:intergenic 25.0%
! AATGGAACAGATAAAATTGT+AGG + chr3_4:74954195-74954214 None:intergenic 25.0%
! CAAAAGTAGAAAAATTGTCT+TGG + chr3_4:74954557-74954576 None:intergenic 25.0%
!! CAAGACAATTTTTCTACTTT+TGG - chr3_4:74954555-74954574 Msa0521930:intron 25.0%
!!! TATAGTCTTTTATTTAGTCG+TGG - chr3_4:74954688-74954707 Msa0521930:intron 25.0%
!!! ATAGTCTTTTATTTAGTCGT+GGG - chr3_4:74954689-74954708 Msa0521930:intron 25.0%
! ATAAGAAAATGTGAGGTATA+TGG - chr3_4:74954714-74954733 Msa0521930:intron 25.0%
! TAAGAAAATGTGAGGTATAT+GGG - chr3_4:74954715-74954734 Msa0521930:intron 25.0%
!! GAGTGAATTTTGAATTTCAA+GGG - chr3_4:74954745-74954764 Msa0521930:intron 25.0%
!! TCTTTTTATACGTCCATATT+AGG + chr3_4:74955479-74955498 None:intergenic 25.0%
!!! GTTTTTGATTCAACGTTATT+TGG + chr3_4:74955677-74955696 None:intergenic 25.0%
! TCTCTTTGATTTCTCTAATG+AGG - chr3_4:74953889-74953908 Msa0521930:CDS 30.0%
ATATATATAATGGCACGTAC+CGG + chr3_4:74954067-74954086 None:intergenic 30.0%
TATCTGTTCCATTGAATTTG+AGG - chr3_4:74954202-74954221 Msa0521930:CDS 30.0%
ATCTGTTCCATTGAATTTGA+GGG - chr3_4:74954203-74954222 Msa0521930:CDS 30.0%
CACACATATTAAAGAAAAGC+AGG + chr3_4:74954343-74954362 None:intergenic 30.0%
! GGAGTGAATTTTGAATTTCA+AGG - chr3_4:74954744-74954763 Msa0521930:intron 30.0%
AAATGATTCCTCAGCAAATT+TGG + chr3_4:74955606-74955625 None:intergenic 30.0%
ATATCAACTGTAGCTTATGA+AGG + chr3_4:74955640-74955659 None:intergenic 30.0%
! ACAGTTGATATCTGGATTAA+AGG - chr3_4:74955649-74955668 Msa0521930:intron 30.0%
ATTAATATTCAAAGCTGCGT+TGG + chr3_4:74956284-74956303 None:intergenic 30.0%
CGTCATAAACTAAAAATGGT+AGG + chr3_4:74956340-74956359 None:intergenic 30.0%
CTGTCGTCATAAACTAAAAA+TGG + chr3_4:74956344-74956363 None:intergenic 30.0%
!!! CATTTTTAGTTTATGACGAC+AGG - chr3_4:74956342-74956361 Msa0521930:five_prime_UTR 30.0%
ACAAAGTGTGACAAAGTTTG+TGG + chr3_4:74953667-74953686 None:intergenic 35.0%
! GTGTTGTTGTTGTTGTTGTT+AGG + chr3_4:74953752-74953771 None:intergenic 35.0%
AAAAACTCTTGAGCTTCCAT+GGG + chr3_4:74953782-74953801 None:intergenic 35.0%
AGTGTTTACATTGTTTGTGG+TGG + chr3_4:74953840-74953859 None:intergenic 35.0%
AGCAGTGTTTACATTGTTTG+TGG + chr3_4:74953843-74953862 None:intergenic 35.0%
TCGGTGAAAAAATTGGAAGT+GGG + chr3_4:74953992-74954011 None:intergenic 35.0%
CGAATTGTCGGTGAAAAAAT+TGG + chr3_4:74953999-74954018 None:intergenic 35.0%
TTGACTCAACATGTGTAGTT+AGG - chr3_4:74954121-74954140 Msa0521930:CDS 35.0%
TAGGTTTCCCTCAAATTCAA+TGG + chr3_4:74954213-74954232 None:intergenic 35.0%
GTGGGAAATAAGAAAATGTG+AGG - chr3_4:74954707-74954726 Msa0521930:intron 35.0%
!!! TGAGGAATCATTTTCAAGTG+AGG - chr3_4:74955613-74955632 Msa0521930:intron 35.0%
! CATAAGCTACAGTTGATATC+TGG - chr3_4:74955641-74955660 Msa0521930:intron 35.0%
GCTTAGTCAACACAAATGTT+GGG + chr3_4:74956054-74956073 None:intergenic 35.0%
TGCTTAGTCAACACAAATGT+TGG + chr3_4:74956055-74956074 None:intergenic 35.0%
!! TTGCAACAATTGGTGTTGTT+GGG + chr3_4:74956134-74956153 None:intergenic 35.0%
GATGTTGTAGTTGCAACAAT+TGG + chr3_4:74956144-74956163 None:intergenic 35.0%
CATCACAGCATTATGTTTGA+AGG - chr3_4:74956163-74956182 Msa0521930:CDS 35.0%
AATCAAGTAATCATCACCGT+TGG + chr3_4:74956194-74956213 None:intergenic 35.0%
TTACTTGATTCATCAACACG+TGG - chr3_4:74956204-74956223 Msa0521930:CDS 35.0%
TACTTGATTCATCAACACGT+GGG - chr3_4:74956205-74956224 Msa0521930:CDS 35.0%
GTGTGACAAAGTTTGTGGTT+TGG + chr3_4:74953662-74953681 None:intergenic 40.0%
GAAAAACTCTTGAGCTTCCA+TGG + chr3_4:74953783-74953802 None:intergenic 40.0%
! GTTTGTGGTGGAATTGTTGT+CGG + chr3_4:74953828-74953847 None:intergenic 40.0%
ATCTTCAACGATGAAGTGGT+CGG + chr3_4:74953870-74953889 None:intergenic 40.0%
AGAGATCTTCAACGATGAAG+TGG + chr3_4:74953874-74953893 None:intergenic 40.0%
!! GAATCATTAGAGTTGGTAGG+AGG + chr3_4:74953950-74953969 None:intergenic 40.0%
! GGAGAATCATTAGAGTTGGT+AGG + chr3_4:74953953-74953972 None:intergenic 40.0%
AGGAGGAGAATCATTAGAGT+TGG + chr3_4:74953957-74953976 None:intergenic 40.0%
! GAAGTGGGATTGATTTCAAG+AGG + chr3_4:74953977-74953996 None:intergenic 40.0%
GTCGGTGAAAAAATTGGAAG+TGG + chr3_4:74953993-74954012 None:intergenic 40.0%
AGAATTCTGGCACGAATTGT+CGG + chr3_4:74954011-74954030 None:intergenic 40.0%
ATGTGAGGTATATGGGATAG+AGG - chr3_4:74954722-74954741 Msa0521930:intron 40.0%
TGTGAGGTATATGGGATAGA+GGG - chr3_4:74954723-74954742 Msa0521930:intron 40.0%
CCTTATGATACACACCTACA+CGG - chr3_4:74955404-74955423 Msa0521930:intron 40.0%
ATATCTTATGTTCGCCGTGT+AGG + chr3_4:74955421-74955440 None:intergenic 40.0%
!! ATGGATGTCCAAATTTGCTG+AGG - chr3_4:74955595-74955614 Msa0521930:intron 40.0%
! ACACTTGAGGGTGTATTGTT+GGG + chr3_4:74955715-74955734 None:intergenic 40.0%
!!! TTTCTTGGAGAGGGCTTTTT+TGG + chr3_4:74955866-74955885 None:intergenic 40.0%
! AGGTCTCTTTTTCTTGGAGA+GGG + chr3_4:74955875-74955894 None:intergenic 40.0%
TCTCCAAGAAAAAGAGACCT+CGG - chr3_4:74955875-74955894 Msa0521930:intron 40.0%
ACGCATTGCATAGTGTCTTT+GGG + chr3_4:74955976-74955995 None:intergenic 40.0%
!! GTTGCAACAATTGGTGTTGT+TGG + chr3_4:74956135-74956154 None:intergenic 40.0%
ATGTTTGAAGGACCATCCAA+CGG - chr3_4:74956175-74956194 Msa0521930:CDS 40.0%
AAGTAATCATCACCGTTGGA+TGG + chr3_4:74956190-74956209 None:intergenic 40.0%
AATATTCAAAGCTGCGTTGG+AGG + chr3_4:74956281-74956300 None:intergenic 40.0%
ATTGTGGACAGAAGCTGGTT+TGG + chr3_4:74953805-74953824 None:intergenic 45.0%
GTGGAATTGTTGTCGGATTG+TGG + chr3_4:74953821-74953840 None:intergenic 45.0%
TTCGAAAGTGGGGTCTTCAA+TGG + chr3_4:74953921-74953940 None:intergenic 45.0%
GAGGAGATTCTTCGAAAGTG+GGG + chr3_4:74953931-74953950 None:intergenic 45.0%
GGAGGAGATTCTTCGAAAGT+GGG + chr3_4:74953932-74953951 None:intergenic 45.0%
AGGAGGAGATTCTTCGAAAG+TGG + chr3_4:74953933-74953952 None:intergenic 45.0%
! GTGGGATTGATTTCAAGAGG+AGG + chr3_4:74953974-74953993 None:intergenic 45.0%
TATAATGGCACGTACCGGAA+CGG + chr3_4:74954062-74954081 None:intergenic 45.0%
TGGATTCTTAACAAGTGCCC+CGG - chr3_4:74954575-74954594 Msa0521930:intron 45.0%
CCGTGTAGGTGTGTATCATA+AGG + chr3_4:74955407-74955426 None:intergenic 45.0%
ATGATCTTGCGCAGCTAGAA+TGG - chr3_4:74955576-74955595 Msa0521930:intron 45.0%
! GACACTTGAGGGTGTATTGT+TGG + chr3_4:74955716-74955735 None:intergenic 45.0%
TGGTTGGAGAGAGAACTAGA+AGG + chr3_4:74955799-74955818 None:intergenic 45.0%
ATCTTCTCACTCTGACACCA+TGG - chr3_4:74955835-74955854 Msa0521930:intron 45.0%
! GAGGTCTCTTTTTCTTGGAG+AGG + chr3_4:74955876-74955895 None:intergenic 45.0%
! TCACCGAGGTCTCTTTTTCT+TGG + chr3_4:74955881-74955900 None:intergenic 45.0%
AGAAAAAGAGACCTCGGTGA+TGG - chr3_4:74955881-74955900 Msa0521930:intron 45.0%
CATAGTGTCTTTGGGCCTAA+TGG + chr3_4:74955968-74955987 None:intergenic 45.0%
CACGCATTGCATAGTGTCTT+TGG + chr3_4:74955977-74955996 None:intergenic 45.0%
AAGACACTATGCAATGCGTG+TGG - chr3_4:74955977-74955996 Msa0521930:intron 45.0%
TGTGGAGTCAGATACAAGTC+AGG - chr3_4:74955995-74956014 Msa0521930:intron 45.0%
TTGCACCTTTCGATGTGAGT+TGG + chr3_4:74956080-74956099 None:intergenic 45.0%
TACATGAGGTGCGTGAAATC+GGG + chr3_4:74956232-74956251 None:intergenic 45.0%
CTACATGAGGTGCGTGAAAT+CGG + chr3_4:74956233-74956252 None:intergenic 45.0%
TTCACGCACCTCATGTAGTA+CGG - chr3_4:74956235-74956254 Msa0521930:CDS 45.0%
!! AATAATTACTATATATATAA+TGG + chr3_4:74954077-74954096 None:intergenic 5.0%
!!! TAAAATGAATATTATTTTTA+AGG - chr3_4:74954448-74954467 Msa0521930:CDS 5.0%
!! ATAGTTAAAAAAAAAATATA+GGG + chr3_4:74955006-74955025 None:intergenic 5.0%
!! TATAGTTAAAAAAAAAATAT+AGG + chr3_4:74955007-74955026 None:intergenic 5.0%
!!! ATAAATTTTATTATATTTGA+TGG + chr3_4:74955178-74955197 None:intergenic 5.0%
CAACAACACCTCCTAACCCA+TGG - chr3_4:74953763-74953782 Msa0521930:three_prime_UTR 50.0%
!! TTGAGCTTCCATGGGTTAGG+AGG + chr3_4:74953774-74953793 None:intergenic 50.0%
CTCTTGAGCTTCCATGGGTT+AGG + chr3_4:74953777-74953796 None:intergenic 50.0%
ATTCTTAACAAGTGCCCCGG+AGG - chr3_4:74954578-74954597 Msa0521930:intron 50.0%
TCTTGTTAACCAGTGCCTCC+GGG + chr3_4:74954596-74954615 None:intergenic 50.0%
! GTCTTGTTAACCAGTGCCTC+CGG + chr3_4:74954597-74954616 None:intergenic 50.0%
! GTTTACTACGGGCTTTTGCC+GGG + chr3_4:74955742-74955761 None:intergenic 50.0%
! CGTTTACTACGGGCTTTTGC+CGG + chr3_4:74955743-74955762 None:intergenic 50.0%
CGGCAAAAGCCCGTAGTAAA+CGG - chr3_4:74955741-74955760 Msa0521930:intron 50.0%
CACACGTGACCGTTTACTAC+GGG + chr3_4:74955753-74955772 None:intergenic 50.0%
GCACACGTGACCGTTTACTA+CGG + chr3_4:74955754-74955773 None:intergenic 50.0%
AGAGTGAGAAGATGTGGTGG+TGG + chr3_4:74955828-74955847 None:intergenic 50.0%
GTCAGAGTGAGAAGATGTGG+TGG + chr3_4:74955831-74955850 None:intergenic 50.0%
GGTGTCAGAGTGAGAAGATG+TGG + chr3_4:74955834-74955853 None:intergenic 50.0%
!!! CTTGGAGAGGGCTTTTTTGG+TGG + chr3_4:74955863-74955882 None:intergenic 50.0%
! AAAAGAGACCTCGGTGATGG+AGG - chr3_4:74955884-74955903 Msa0521930:intron 50.0%
! CCACTGTGGAGTCTTATCAG+TGG + chr3_4:74955936-74955955 None:intergenic 50.0%
CCACTGATAAGACTCCACAG+TGG - chr3_4:74955933-74955952 Msa0521930:intron 50.0%
!! GTCGACTCGTACCTGAGTAT+AGG - chr3_4:74956017-74956036 Msa0521930:CDS 50.0%
!! ACAAATGTTGGGCTTGCTGC+AGG + chr3_4:74956043-74956062 None:intergenic 50.0%
GCACTCCAACTCACATCGAA+AGG - chr3_4:74956072-74956091 Msa0521930:CDS 50.0%
TCATCATCTCCTTCTGGCGT+CGG + chr3_4:74956108-74956127 None:intergenic 50.0%
ACGCCAGAAGGAGATGATGA+GGG - chr3_4:74956108-74956127 Msa0521930:CDS 50.0%
!! CCAAAGCACCGTACTACATG+AGG + chr3_4:74956246-74956265 None:intergenic 50.0%
CCTCATGTAGTACGGTGCTT+TGG - chr3_4:74956243-74956262 Msa0521930:CDS 50.0%
GTCGGATTGTGGACAGAAGC+TGG + chr3_4:74953810-74953829 None:intergenic 55.0%
TCGTGCCAGAATTCTGCCGA+CGG - chr3_4:74954016-74954035 Msa0521930:CDS 55.0%
CTTGTTAACCAGTGCCTCCG+GGG + chr3_4:74954595-74954614 None:intergenic 55.0%
ACACCCTCAAGTGTCTGTCC+CGG - chr3_4:74955721-74955740 Msa0521930:intron 55.0%
TTGCCGGGACAGACACTTGA+GGG + chr3_4:74955727-74955746 None:intergenic 55.0%
TTTGCCGGGACAGACACTTG+AGG + chr3_4:74955728-74955747 None:intergenic 55.0%
!! GTGAGAAGATGTGGTGGTGG+TGG + chr3_4:74955825-74955844 None:intergenic 55.0%
!!! GGGCTTTTTTGGTGGAGCCA+TGG + chr3_4:74955855-74955874 None:intergenic 55.0%
!! AGACCTCGGTGATGGAGGAA+AGG - chr3_4:74955889-74955908 Msa0521930:intron 55.0%
!! GACCTCGGTGATGGAGGAAA+GGG - chr3_4:74955890-74955909 Msa0521930:intron 55.0%
TGTCTTTGGGCCTAATGGCC+CGG + chr3_4:74955963-74955982 None:intergenic 55.0%
CTTGCTGCAGGCCTATACTC+AGG + chr3_4:74956031-74956050 None:intergenic 55.0%
GACGCCAGAAGGAGATGATG+AGG - chr3_4:74956107-74956126 Msa0521930:CDS 55.0%
AAGGGCCGTCGGCAGAATTC+TGG + chr3_4:74954024-74954043 None:intergenic 60.0%
CGGAGAGTTCGCCGGAGAAA+GGG + chr3_4:74954042-74954061 None:intergenic 60.0%
ACGGAGAGTTCGCCGGAGAA+AGG + chr3_4:74954043-74954062 None:intergenic 60.0%
TACCGGAACGGAGAGTTCGC+CGG + chr3_4:74954050-74954069 None:intergenic 60.0%
CACGTGTGCCTCCATGCAAC+TGG - chr3_4:74955765-74955784 Msa0521930:intron 60.0%
CGCGAAGTCCAGTTGCATGG+AGG + chr3_4:74955776-74955795 None:intergenic 60.0%
AGGCGCGAAGTCCAGTTGCA+TGG + chr3_4:74955779-74955798 None:intergenic 60.0%
!! AGATGTGGTGGTGGTGGTCG+TGG + chr3_4:74955819-74955838 None:intergenic 60.0%
GACCCTTTCCTCCATCACCG+AGG + chr3_4:74955895-74955914 None:intergenic 60.0%
AAGACTCCACAGTGGCGCAC+CGG - chr3_4:74955941-74955960 Msa0521930:intron 60.0%
GGGCCCTCATCATCTCCTTC+TGG + chr3_4:74956114-74956133 None:intergenic 60.0%
TCTGCCGACGGCCCTTTCTC+CGG - chr3_4:74954028-74954047 Msa0521930:CDS 65.0%
TTCGCCGGAGAAAGGGCCGT+CGG + chr3_4:74954035-74954054 None:intergenic 65.0%
CTCCGGCGAACTCTCCGTTC+CGG - chr3_4:74954045-74954064 Msa0521930:CDS 65.0%
! AACAAGTGCCCCGGAGGCAC+TGG - chr3_4:74954584-74954603 Msa0521930:intron 65.0%
!! GTGGTGGTGGTGGTCGTGGT+TGG + chr3_4:74955815-74955834 None:intergenic 65.0%
AGACTCCACAGTGGCGCACC+GGG - chr3_4:74955942-74955961 Msa0521930:intron 65.0%
GCAAGAGCTCCGACGCCAGA+AGG - chr3_4:74956096-74956115 Msa0521930:CDS 65.0%
AATGGCCCGGTGCGCCACTG+TGG + chr3_4:74955950-74955969 None:intergenic 70.0%
CAGTGGCGCACCGGGCCATT+AGG - chr3_4:74955950-74955969 Msa0521930:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 74953633 74956438 74953633 ID=Msa0521930;Name=Msa0521930
chr3_4 mRNA 74953633 74956438 74953633 ID=Msa0521930-mRNA-1;Parent=Msa0521930;Name=Msa0521930-mRNA-1;_AED=0.01;_eAED=0.01;_QI=149|1|1|1|1|1|2|186|322
chr3_4 exon 74953633 74954502 74953633 ID=Msa0521930-mRNA-1:exon:21335;Parent=Msa0521930-mRNA-1
chr3_4 exon 74956005 74956438 74956005 ID=Msa0521930-mRNA-1:exon:21334;Parent=Msa0521930-mRNA-1
chr3_4 five_prime_UTR 74956290 74956438 74956290 ID=Msa0521930-mRNA-1:five_prime_utr;Parent=Msa0521930-mRNA-1
chr3_4 CDS 74956005 74956289 74956005 ID=Msa0521930-mRNA-1:cds;Parent=Msa0521930-mRNA-1
chr3_4 CDS 74953819 74954502 74953819 ID=Msa0521930-mRNA-1:cds;Parent=Msa0521930-mRNA-1
chr3_4 three_prime_UTR 74953633 74953818 74953633 ID=Msa0521930-mRNA-1:three_prime_utr;Parent=Msa0521930-mRNA-1
Gene Sequence

>Msa0521930

ATGGAAGCTCAAGAGTTTTTCCAAACCAGCTTCTGTCCACAATCCGACAACAATTCCACCACAAACAATGTAAACACTGCTGCTTCCGACCACTTCATCGTTGAAGATCTCTTTGATTTCTCTAATGAGGATGTTGCCATTGAAGACCCCACTTTCGAAGAATCTCCTCCTACCAACTCTAATGATTCTCCTCCTCTTGAAATCAATCCCACTTCCAATTTTTTCACCGACAATTCGTGCCAGAATTCTGCCGACGGCCCTTTCTCCGGCGAACTCTCCGTTCCGCAATATGATGATCTTGCGCAGCTAGAATGGATGTCCAAATTTGCTGAGGAATCATTTTCAAGTGAGGACCTTCATAAGCTACAGTTGATATCTGGATTAAAGGCACCAAATAACGTTGAATCAAAAACTCGTGAAGAACGCAACCCAACAATACACCCTCAAGTGTCTGTCCCGGCAAAAGCCCGTAGTAAACGGTCACGTGTGCCTCCATGCAACTGGACTTCGCGCCTTCTAGTTCTCTCTCCAACCACGACCACCACCACCACATCTTCTCACTCTGACACCATGGCTCCACCAAAAAAGCCCTCTCCAAGAAAAAGAGACCTCGGTGATGGAGGAAAGGGTCGCAAGTGTCTTCATTGCGCCACTGATAAGACTCCACAGTGGCGCACCGGGCCATTAGGCCCAAAGACACTATGCAATGCGTGTGGAGTCAGATACAAGTCAGGTCGACTCGTACCTGAGTATAGGCCTGCAGCAAGCCCAACATTTGTGTTGACTAAGCACTCCAACTCACATCGAAAGGTGCAAGAGCTCCGACGCCAGAAGGAGATGATGAGGGCCCAACAACACCAATTGTTGCAACTACAACATCATCACAGCATTATGTTTGAAGGACCATCCAACGGTGATGATTACTTGATTCATCAACACGTGGGACCCGATTTCACGCACCTCATGTAG
Protein sequence

>Msa0521930

MEAQEFFQTSFCPQSDNNSTTNNVNTAASDHFIVEDLFDFSNEDVAIEDPTFEESPPTNSNDSPPLEINPTSNFFTDNSCQNSADGPFSGELSVPQYDDLAQLEWMSKFAEESFSSEDLHKLQLISGLKAPNNVESKTREERNPTIHPQVSVPAKARSKRSRVPPCNWTSRLLVLSPTTTTTTTSSHSDTMAPPKKPSPRKRDLGDGGKGRKCLHCATDKTPQWRTGPLGPKTLCNACGVRYKSGRLVPEYRPAASPTFVLTKHSNSHRKVQELRRQKEMMRAQQHQLLQLQHHHSIMFEGPSNGDDYLIHQHVGPDFTHLM*