AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0528850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0528850 MtrunA17_Chr4g0042721 97.391 115 3 0 1 115 1 115 4.22e-83 243
Msa0528850 MtrunA17_Chr6g0453521 65.517 87 30 0 22 108 17 103 2.11e-40 135
Msa0528850 MtrunA17_Chr5g0417891 50.000 122 57 3 1 119 1 121 1.09e-39 133
Msa0528850 MtrunA17_Chr5g0417931 52.212 113 47 5 1 107 3 114 1.82e-33 117
Msa0528850 MtrunA17_Chr5g0417901 34.161 161 97 4 1 158 3 157 8.30e-27 100
Msa0528850 MtrunA17_Chr7g0217901 58.824 68 23 1 13 75 4 71 1.02e-22 85.9
Msa0528850 MtrunA17_Chr3g0114751 40.000 100 51 3 15 108 14 110 6.66e-21 85.1
Msa0528850 MtrunA17_Chr2g0277981 40.594 101 55 3 12 112 7 102 4.69e-20 82.4
Msa0528850 MtrunA17_Chr5g0423921 40.659 91 48 2 25 115 27 111 3.60e-19 80.5
Msa0528850 MtrunA17_Chr8g0380801 35.354 99 52 2 15 109 13 103 5.31e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0528850 AT1G26690.1 64.706 102 36 0 14 115 12 113 2.32e-46 150
Msa0528850 AT1G69460.1 62.745 102 38 0 14 115 12 113 2.12e-43 143
Msa0528850 AT1G14010.1 57.692 104 44 0 12 115 8 111 3.87e-41 137
Msa0528850 AT3G29070.1 54.839 93 42 0 25 117 34 126 6.58e-33 116
Msa0528850 AT2G03040.1 55.914 93 40 1 24 115 20 112 5.25e-32 112
Msa0528850 AT2G03290.1 55.914 93 40 1 24 115 20 112 6.00e-32 114
Msa0528850 AT1G09580.1 38.095 105 59 2 8 111 12 111 5.40e-19 80.5
Msa0528850 AT1G21900.1 35.714 98 59 2 12 108 13 107 1.24e-16 73.9
Msa0528850 AT1G57620.1 30.928 97 59 3 14 107 11 102 1.35e-11 60.5

Find 27 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGTCTTCTGAGATGCATT+TGG 0.409852 4_1:-1813393 None:intergenic
GGTAAAGGGTAACCCTCGTT+GGG 0.443213 4_1:-1813458 None:intergenic
TATGGAGATCGTGTTCAATC+TGG 0.449834 4_1:+1813626 Msa0528850:CDS
GCATTTGTTGCTGCAGAAGC+TGG 0.459925 4_1:+1813656 Msa0528850:CDS
CATGGAAATAACTATCACTA+TGG 0.460188 4_1:+1813608 Msa0528850:CDS
ACGGTGATTCTGTGTGAATC+AGG 0.470784 4_1:-1813479 None:intergenic
TTCTGTGTGAATCAGGTAAA+GGG 0.476873 4_1:-1813472 None:intergenic
GGAGGAAGAAGAAAGAGTAA+AGG 0.484211 4_1:+1813998 Msa0528850:CDS
AGGAGTAGAAGTAGAAGAAG+AGG 0.488866 4_1:+1813977 Msa0528850:CDS
ATTCAGTGGAAGATGAAAAC+AGG 0.491337 4_1:-1813340 None:intergenic
AGAAGAAAGAGTAAAGGAAG+AGG 0.492062 4_1:+1814004 Msa0528850:CDS
AAAAGCAATTCCATGACTGT+TGG 0.505073 4_1:+1813416 Msa0528850:CDS
ATTCTGTGTGAATCAGGTAA+AGG 0.528712 4_1:-1813473 None:intergenic
GTTTAGGTTACATCGTCTCA+TGG 0.531885 4_1:+1813590 Msa0528850:intron
AAGATTTGAGTTCAAATCTG+AGG 0.534501 4_1:-1813364 None:intergenic
AGATTTGAGTTCAAATCTGA+GGG 0.538909 4_1:-1813363 None:intergenic
AGTAGAAGTAGAAGAAGAGG+AGG 0.539062 4_1:+1813980 Msa0528850:CDS
ATTCACACAGAATCACCGTC+AGG 0.545208 4_1:+1813483 Msa0528850:CDS
TTCAAATCTGAGGGATTCAG+TGG 0.571693 4_1:-1813354 None:intergenic
AGGTAAAGGGTAACCCTCGT+TGG 0.582707 4_1:-1813459 None:intergenic
GGTGTACTTTCCAACAGTCA+TGG 0.589902 4_1:-1813426 None:intergenic
ACCCTCGTTGGGGTTAACGA+TGG 0.671660 4_1:-1813447 None:intergenic
TTCACACAGAATCACCGTCA+GGG 0.676656 4_1:+1813484 Msa0528850:CDS
GGCTGCTGAGAATAAACCTG+AGG 0.691504 4_1:+1813700 Msa0528850:CDS
GTAAAGGGTAACCCTCGTTG+GGG 0.701463 4_1:-1813457 None:intergenic
CACCATCGTTAACCCCAACG+AGG 0.704046 4_1:+1813445 Msa0528850:CDS
ACCATCGTTAACCCCAACGA+GGG 0.730690 4_1:+1813446 Msa0528850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTGATTTTTTTTTTC+TGG + chr4_1:1813567-1813586 Msa0528850:intron 10.0%
!!! AATTTTGATTTTTTTTTTCT+GGG + chr4_1:1813568-1813587 Msa0528850:intron 10.0%
!!! AAATTTTGATTTTTTTTTTC+TGG + chr4_1:1813567-1813586 Msa0528850:intron 10.0%
!!! AATTTTGATTTTTTTTTTCT+GGG + chr4_1:1813568-1813587 Msa0528850:intron 10.0%
!! NNNNNNNNAGACACTTTATT+AGG + chr4_1:1813844-1813863 Msa0528850:intron 15.0%
!! NNNNNNNNAGACACTTTATT+AGG + chr4_1:1813844-1813863 Msa0528850:intron 15.0%
!!! ATTCAACTGTTTTTGATTAA+TGG + chr4_1:1813537-1813556 Msa0528850:intron 20.0%
!!! TTCAACTGTTTTTGATTAAT+GGG + chr4_1:1813538-1813557 Msa0528850:intron 20.0%
!!! ATTCAACTGTTTTTGATTAA+TGG + chr4_1:1813537-1813556 Msa0528850:intron 20.0%
!!! TTCAACTGTTTTTGATTAAT+GGG + chr4_1:1813538-1813557 Msa0528850:intron 20.0%
!! AAAGATGAAAAAAGGGTTTT+GGG - chr4_1:1813304-1813323 None:intergenic 25.0%
! AGAAGAGAAAGATGAAAAAA+GGG - chr4_1:1813311-1813330 None:intergenic 25.0%
! TAGAAGAGAAAGATGAAAAA+AGG - chr4_1:1813312-1813331 None:intergenic 25.0%
!!! GATTTTTTTTTTCTGGGTTT+AGG + chr4_1:1813574-1813593 Msa0528850:intron 25.0%
!!! TGATTACATGACTTGTTTTT+GGG + chr4_1:1813679-1813698 Msa0528850:CDS 25.0%
!! TCTTTTTCTTTGAATCATTG+AGG - chr4_1:1813898-1813917 None:intergenic 25.0%
!! AAAGATGAAAAAAGGGTTTT+GGG - chr4_1:1813304-1813323 None:intergenic 25.0%
! AGAAGAGAAAGATGAAAAAA+GGG - chr4_1:1813311-1813330 None:intergenic 25.0%
! TAGAAGAGAAAGATGAAAAA+AGG - chr4_1:1813312-1813331 None:intergenic 25.0%
!!! GATTTTTTTTTTCTGGGTTT+AGG + chr4_1:1813574-1813593 Msa0528850:intron 25.0%
!!! TGATTACATGACTTGTTTTT+GGG + chr4_1:1813679-1813698 Msa0528850:CDS 25.0%
!! TCTTTTTCTTTGAATCATTG+AGG - chr4_1:1813898-1813917 None:intergenic 25.0%
! GAAAGATGAAAAAAGGGTTT+TGG - chr4_1:1813305-1813324 None:intergenic 30.0%
! AGATTTGAGTTCAAATCTGA+GGG - chr4_1:1813366-1813385 None:intergenic 30.0%
! AAGATTTGAGTTCAAATCTG+AGG - chr4_1:1813367-1813386 None:intergenic 30.0%
GAATTTGATTCAAAGCAGTT+TGG - chr4_1:1813521-1813540 None:intergenic 30.0%
CATGGAAATAACTATCACTA+TGG + chr4_1:1813608-1813627 Msa0528850:CDS 30.0%
!!! GTGATTACATGACTTGTTTT+TGG + chr4_1:1813678-1813697 Msa0528850:CDS 30.0%
! GAAAGATGAAAAAAGGGTTT+TGG - chr4_1:1813305-1813324 None:intergenic 30.0%
! AGATTTGAGTTCAAATCTGA+GGG - chr4_1:1813366-1813385 None:intergenic 30.0%
! AAGATTTGAGTTCAAATCTG+AGG - chr4_1:1813367-1813386 None:intergenic 30.0%
GAATTTGATTCAAAGCAGTT+TGG - chr4_1:1813521-1813540 None:intergenic 30.0%
CATGGAAATAACTATCACTA+TGG + chr4_1:1813608-1813627 Msa0528850:CDS 30.0%
!!! GTGATTACATGACTTGTTTT+TGG + chr4_1:1813678-1813697 Msa0528850:CDS 30.0%
!!! GAAAAAAGGGTTTTGGGTTA+AGG - chr4_1:1813298-1813317 None:intergenic 35.0%
ATTCAGTGGAAGATGAAAAC+AGG - chr4_1:1813343-1813362 None:intergenic 35.0%
AAAAGCAATTCCATGACTGT+TGG + chr4_1:1813416-1813435 Msa0528850:CDS 35.0%
TTCTGTGTGAATCAGGTAAA+GGG - chr4_1:1813475-1813494 None:intergenic 35.0%
ATTCTGTGTGAATCAGGTAA+AGG - chr4_1:1813476-1813495 None:intergenic 35.0%
!! CGTTGACTATTGATTTGGAA+TGG + chr4_1:1813726-1813745 Msa0528850:intron 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG + chr4_1:1813954-1813973 Msa0528850:CDS 35.0%
AGAAGAAAGAGTAAAGGAAG+AGG + chr4_1:1814004-1814023 Msa0528850:CDS 35.0%
!!! GAAAAAAGGGTTTTGGGTTA+AGG - chr4_1:1813298-1813317 None:intergenic 35.0%
ATTCAGTGGAAGATGAAAAC+AGG - chr4_1:1813343-1813362 None:intergenic 35.0%
AAAAGCAATTCCATGACTGT+TGG + chr4_1:1813416-1813435 Msa0528850:CDS 35.0%
TTCTGTGTGAATCAGGTAAA+GGG - chr4_1:1813475-1813494 None:intergenic 35.0%
ATTCTGTGTGAATCAGGTAA+AGG - chr4_1:1813476-1813495 None:intergenic 35.0%
!! CGTTGACTATTGATTTGGAA+TGG + chr4_1:1813726-1813745 Msa0528850:intron 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG + chr4_1:1813954-1813973 Msa0528850:CDS 35.0%
AGAAGAAAGAGTAAAGGAAG+AGG + chr4_1:1814004-1814023 Msa0528850:CDS 35.0%
TTCAAATCTGAGGGATTCAG+TGG - chr4_1:1813357-1813376 None:intergenic 40.0%
!! GATGTCTTCTGAGATGCATT+TGG - chr4_1:1813396-1813415 None:intergenic 40.0%
! GTTTAGGTTACATCGTCTCA+TGG + chr4_1:1813590-1813609 Msa0528850:intron 40.0%
TATGGAGATCGTGTTCAATC+TGG + chr4_1:1813626-1813645 Msa0528850:CDS 40.0%
! GGTGACGTTGACTATTGATT+TGG + chr4_1:1813721-1813740 Msa0528850:intron 40.0%
AGGAGTAGAAGTAGAAGAAG+AGG + chr4_1:1813977-1813996 Msa0528850:CDS 40.0%
AGTAGAAGTAGAAGAAGAGG+AGG + chr4_1:1813980-1813999 Msa0528850:CDS 40.0%
GGAGGAAGAAGAAAGAGTAA+AGG + chr4_1:1813998-1814017 Msa0528850:CDS 40.0%
TTCAAATCTGAGGGATTCAG+TGG - chr4_1:1813357-1813376 None:intergenic 40.0%
!! GATGTCTTCTGAGATGCATT+TGG - chr4_1:1813396-1813415 None:intergenic 40.0%
! GTTTAGGTTACATCGTCTCA+TGG + chr4_1:1813590-1813609 Msa0528850:intron 40.0%
TATGGAGATCGTGTTCAATC+TGG + chr4_1:1813626-1813645 Msa0528850:CDS 40.0%
! GGTGACGTTGACTATTGATT+TGG + chr4_1:1813721-1813740 Msa0528850:intron 40.0%
AGGAGTAGAAGTAGAAGAAG+AGG + chr4_1:1813977-1813996 Msa0528850:CDS 40.0%
AGTAGAAGTAGAAGAAGAGG+AGG + chr4_1:1813980-1813999 Msa0528850:CDS 40.0%
GGAGGAAGAAGAAAGAGTAA+AGG + chr4_1:1813998-1814017 Msa0528850:CDS 40.0%
GGTGTACTTTCCAACAGTCA+TGG - chr4_1:1813429-1813448 None:intergenic 45.0%
ACGGTGATTCTGTGTGAATC+AGG - chr4_1:1813482-1813501 None:intergenic 45.0%
ATTCACACAGAATCACCGTC+AGG + chr4_1:1813483-1813502 Msa0528850:CDS 45.0%
TTCACACAGAATCACCGTCA+GGG + chr4_1:1813484-1813503 Msa0528850:CDS 45.0%
! TGGACTTTTGCTTACCCTGA+CGG - chr4_1:1813501-1813520 None:intergenic 45.0%
TCAATAGTCAACGTCACCTC+AGG - chr4_1:1813719-1813738 None:intergenic 45.0%
ACAATGCTTCCCCTAAGTGT+GGG - chr4_1:1813875-1813894 None:intergenic 45.0%
GGTGTACTTTCCAACAGTCA+TGG - chr4_1:1813429-1813448 None:intergenic 45.0%
ACGGTGATTCTGTGTGAATC+AGG - chr4_1:1813482-1813501 None:intergenic 45.0%
ATTCACACAGAATCACCGTC+AGG + chr4_1:1813483-1813502 Msa0528850:CDS 45.0%
TTCACACAGAATCACCGTCA+GGG + chr4_1:1813484-1813503 Msa0528850:CDS 45.0%
! TGGACTTTTGCTTACCCTGA+CGG - chr4_1:1813501-1813520 None:intergenic 45.0%
TCAATAGTCAACGTCACCTC+AGG - chr4_1:1813719-1813738 None:intergenic 45.0%
ACAATGCTTCCCCTAAGTGT+GGG - chr4_1:1813875-1813894 None:intergenic 45.0%
ACCATCGTTAACCCCAACGA+GGG + chr4_1:1813446-1813465 Msa0528850:CDS 50.0%
GTAAAGGGTAACCCTCGTTG+GGG - chr4_1:1813460-1813479 None:intergenic 50.0%
GGTAAAGGGTAACCCTCGTT+GGG - chr4_1:1813461-1813480 None:intergenic 50.0%
AGGTAAAGGGTAACCCTCGT+TGG - chr4_1:1813462-1813481 None:intergenic 50.0%
! GCATTTGTTGCTGCAGAAGC+TGG + chr4_1:1813656-1813675 Msa0528850:CDS 50.0%
GGCTGCTGAGAATAAACCTG+AGG + chr4_1:1813700-1813719 Msa0528850:CDS 50.0%
AATGCTTCCCCTAAGTGTGG+GGG - chr4_1:1813873-1813892 None:intergenic 50.0%
CAATGCTTCCCCTAAGTGTG+GGG - chr4_1:1813874-1813893 None:intergenic 50.0%
GACAATGCTTCCCCTAAGTG+TGG - chr4_1:1813876-1813895 None:intergenic 50.0%
ACCATCGTTAACCCCAACGA+GGG + chr4_1:1813446-1813465 Msa0528850:CDS 50.0%
GTAAAGGGTAACCCTCGTTG+GGG - chr4_1:1813460-1813479 None:intergenic 50.0%
GGTAAAGGGTAACCCTCGTT+GGG - chr4_1:1813461-1813480 None:intergenic 50.0%
AGGTAAAGGGTAACCCTCGT+TGG - chr4_1:1813462-1813481 None:intergenic 50.0%
! GCATTTGTTGCTGCAGAAGC+TGG + chr4_1:1813656-1813675 Msa0528850:CDS 50.0%
GGCTGCTGAGAATAAACCTG+AGG + chr4_1:1813700-1813719 Msa0528850:CDS 50.0%
AATGCTTCCCCTAAGTGTGG+GGG - chr4_1:1813873-1813892 None:intergenic 50.0%
CAATGCTTCCCCTAAGTGTG+GGG - chr4_1:1813874-1813893 None:intergenic 50.0%
GACAATGCTTCCCCTAAGTG+TGG - chr4_1:1813876-1813895 None:intergenic 50.0%
CACCATCGTTAACCCCAACG+AGG + chr4_1:1813445-1813464 Msa0528850:CDS 55.0%
ACCCTCGTTGGGGTTAACGA+TGG - chr4_1:1813450-1813469 None:intergenic 55.0%
ATGCTTCCCCTAAGTGTGGG+GGG - chr4_1:1813872-1813891 None:intergenic 55.0%
CACCATCGTTAACCCCAACG+AGG + chr4_1:1813445-1813464 Msa0528850:CDS 55.0%
ACCCTCGTTGGGGTTAACGA+TGG - chr4_1:1813450-1813469 None:intergenic 55.0%
ATGCTTCCCCTAAGTGTGGG+GGG - chr4_1:1813872-1813891 None:intergenic 55.0%
ATTAGGCCCCCCCCACACTT+AGG + chr4_1:1813861-1813880 Msa0528850:intron 60.0%
TTAGGCCCCCCCCACACTTA+GGG + chr4_1:1813862-1813881 Msa0528850:intron 60.0%
TGCTTCCCCTAAGTGTGGGG+GGG - chr4_1:1813871-1813890 None:intergenic 60.0%
ATTAGGCCCCCCCCACACTT+AGG + chr4_1:1813861-1813880 Msa0528850:intron 60.0%
TTAGGCCCCCCCCACACTTA+GGG + chr4_1:1813862-1813881 Msa0528850:intron 60.0%
TGCTTCCCCTAAGTGTGGGG+GGG - chr4_1:1813871-1813890 None:intergenic 60.0%
TAGGCCCCCCCCACACTTAG+GGG + chr4_1:1813863-1813882 Msa0528850:intron 65.0%
GCTTCCCCTAAGTGTGGGGG+GGG - chr4_1:1813870-1813889 None:intergenic 65.0%
TAGGCCCCCCCCACACTTAG+GGG + chr4_1:1813863-1813882 Msa0528850:intron 65.0%
GCTTCCCCTAAGTGTGGGGG+GGG - chr4_1:1813870-1813889 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 1813287 1814045 1813287 ID=Msa0528850;Name=Msa0528850
chr4_1 mRNA 1813287 1814045 1813287 ID=Msa0528850-mRNA-1;Parent=Msa0528850;Name=Msa0528850-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0|0.66|1|1|3|0|159
chr4_1 exon 1813287 1813505 1813287 ID=Msa0528850-mRNA-1:exon:556;Parent=Msa0528850-mRNA-1
chr4_1 exon 1813596 1813721 1813596 ID=Msa0528850-mRNA-1:exon:557;Parent=Msa0528850-mRNA-1
chr4_1 exon 1813911 1814045 1813911 ID=Msa0528850-mRNA-1:exon:558;Parent=Msa0528850-mRNA-1
chr4_1 CDS 1813287 1813505 1813287 ID=Msa0528850-mRNA-1:cds;Parent=Msa0528850-mRNA-1
chr4_1 CDS 1813596 1813721 1813596 ID=Msa0528850-mRNA-1:cds;Parent=Msa0528850-mRNA-1
chr4_1 CDS 1813911 1814045 1813911 ID=Msa0528850-mRNA-1:cds;Parent=Msa0528850-mRNA-1
Gene Sequence

>Msa0528850

ATGATTAACCTTAACCCAAAACCCTTTTTTCATCTTTCTCTTCTACTTCTCATCCTGTTTTCATCTTCCACTGAATCCCTCAGATTTGAACTCAAATCTTCTTTAACCAAATGCATCTCAGAAGACATCAAAAGCAATTCCATGACTGTTGGAAAGTACACCATCGTTAACCCCAACGAGGGTTACCCTTTACCTGATTCACACAGAATCACCGTCAGGGTTACATCGTCTCATGGAAATAACTATCACTATGGAGATCGTGTTCAATCTGGTCAGTTTGCATTTGTTGCTGCAGAAGCTGGTGATTACATGACTTGTTTTTGGGCTGCTGAGAATAAACCTGAGAAAAAGAAATTAAAAGAGAAGAAGAAGAAGAAAGAAGAAGAAAGAAGAAGAAGAAGAAGAAGAAGAAGGAGTAGAAGTAGAAGAAGAGGAGGAAGAAGAAAGAGTAAAGGAAGAGGAAAGCTCACAATTTTATAA

Protein sequence

>Msa0528850

MINLNPKPFFHLSLLLLILFSSSTESLRFELKSSLTKCISEDIKSNSMTVGKYTIVNPNEGYPLPDSHRITVRVTSSHGNNYHYGDRVQSGQFAFVAAEAGDYMTCFWAAENKPEKKKLKEKKKKKEEERRRRRRRRRSRSRRRGGRRKSKGRGKLTIL*