AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0539700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0539700 MtrunA17_Chr4g0014781 91.592 559 47 0 1 559 1 559 0.0 1038
Msa0539700 MtrunA17_Chr4g0014801 91.055 559 50 0 1 559 1 559 0.0 1017
Msa0539700 MtrunA17_Chr4g0014871 85.740 561 78 2 1 559 1 561 0.0 998
Msa0539700 MtrunA17_Chr4g0014791 86.786 560 70 2 1 559 1 557 0.0 988
Msa0539700 MtrunA17_Chr4g0014841 87.478 559 68 1 1 559 1 557 0.0 983
Msa0539700 MtrunA17_Chr4g0014861 84.562 434 66 1 125 557 1 434 0.0 768
Msa0539700 MtrunA17_Chr3g0077151 66.972 545 169 4 13 555 12 547 0.0 728
Msa0539700 MtrunA17_Chr8g0353031 47.698 543 252 7 30 551 19 550 2.24e-168 489
Msa0539700 MtrunA17_Chr8g0375311 42.301 565 294 8 10 548 21 579 1.17e-158 465
Msa0539700 MtrunA17_Chr7g0241741 41.593 565 305 7 8 551 1 561 1.54e-157 462
Msa0539700 MtrunA17_Chr7g0261811 41.741 563 304 6 17 558 15 574 7.06e-156 459
Msa0539700 MtrunA17_Chr8g0375291 43.636 550 282 8 17 542 28 573 1.00e-155 457
Msa0539700 MtrunA17_Chr3g0104011 42.301 565 299 8 10 551 3 563 2.94e-154 453
Msa0539700 MtrunA17_Chr4g0005901 40.426 564 311 6 10 552 13 572 1.16e-150 444
Msa0539700 MtrunA17_Chr7g0261791 40.754 557 305 6 17 551 17 570 2.70e-150 444
Msa0539700 MtrunA17_Chr7g0261881 39.432 563 317 6 17 558 15 574 1.21e-149 442
Msa0539700 MtrunA17_Chr4g0008651 39.029 556 312 6 17 552 22 570 1.23e-148 440
Msa0539700 MtrunA17_Chr3g0112611 40.378 582 310 12 3 559 11 580 1.65e-148 439
Msa0539700 MtrunA17_Chr3g0114501 40.502 558 311 7 17 555 10 565 6.85e-147 435
Msa0539700 MtrunA17_Chr3g0112671 38.790 562 309 8 10 551 14 560 3.95e-146 433
Msa0539700 MtrunA17_Chr8g0375351 41.667 564 293 11 17 552 28 583 1.93e-145 431
Msa0539700 MtrunA17_Chr8g0375341 41.071 560 294 11 17 548 28 579 2.42e-144 429
Msa0539700 MtrunA17_Chr1g0166791 40.036 557 308 8 17 551 12 564 9.36e-144 427
Msa0539700 MtrunA17_Chr7g0240381 38.859 561 300 8 17 552 17 559 3.29e-143 426
Msa0539700 MtrunA17_Chr8g0375361 40.531 565 308 8 17 557 27 587 3.61e-142 423
Msa0539700 MtrunA17_Chr1g0166711 40.647 556 305 8 17 551 13 564 2.32e-141 421
Msa0539700 MtrunA17_Chr7g0261771 39.209 556 314 6 17 551 19 571 1.49e-140 419
Msa0539700 MtrunA17_Chr1g0187241 39.318 557 312 7 17 551 14 566 1.75e-140 419
Msa0539700 MtrunA17_Chr8g0375331 39.547 574 310 10 1 548 1 563 5.18e-138 412
Msa0539700 MtrunA17_Chr8g0375321 39.247 558 307 9 17 548 25 576 1.27e-137 411
Msa0539700 MtrunA17_Chr1g0166741 37.803 537 301 8 17 531 15 540 2.45e-131 395
Msa0539700 MtrunA17_Chr7g0261851 37.230 556 325 5 17 551 16 568 3.54e-130 393
Msa0539700 MtrunA17_Chr3g0082631 44.017 468 226 9 111 554 9 464 4.72e-129 385
Msa0539700 MtrunA17_Chr5g0399061 38.261 575 324 7 12 559 10 580 4.75e-128 387
Msa0539700 MtrunA17_Chr5g0422881 38.214 560 305 11 29 554 20 572 1.65e-127 385
Msa0539700 MtrunA17_Chr4g0056431 39.003 582 321 8 8 559 4 581 1.10e-125 381
Msa0539700 MtrunA17_Chr5g0399071 39.236 576 313 10 8 553 4 572 9.02e-125 379
Msa0539700 MtrunA17_Chr3g0142101 38.172 558 312 12 9 545 15 560 2.84e-123 374
Msa0539700 MtrunA17_Chr1g0166771 38.356 511 278 8 48 534 10 507 1.46e-122 371
Msa0539700 MtrunA17_Chr7g0255621 37.071 553 313 10 29 553 20 565 8.29e-120 366
Msa0539700 MtrunA17_Chr6g0451841 38.033 539 298 9 36 552 12 536 5.08e-118 359
Msa0539700 MtrunA17_Chr7g0217461 37.113 582 329 13 10 559 5 581 2.80e-117 359
Msa0539700 MtrunA17_Chr7g0255611 35.789 570 325 12 1 541 7 564 1.05e-116 358
Msa0539700 MtrunA17_Chr7g0255601 34.715 579 343 9 9 558 7 579 4.96e-115 354
Msa0539700 MtrunA17_Chr7g0214461 34.813 563 331 12 23 559 72 624 3.22e-111 345
Msa0539700 MtrunA17_Chr5g0400371 35.938 576 333 11 3 548 86 655 1.20e-110 345
Msa0539700 MtrunA17_Chr3g0142091 36.413 552 316 11 20 548 17 556 2.18e-110 341
Msa0539700 MtrunA17_Chr4g0063591 39.350 554 303 10 16 545 9 553 2.76e-106 330
Msa0539700 MtrunA17_Chr4g0054891 35.450 567 325 12 10 546 91 646 3.76e-106 333
Msa0539700 MtrunA17_Chr5g0415791 34.164 562 326 9 29 556 29 580 5.00e-106 330
Msa0539700 MtrunA17_Chr5g0415961 33.735 581 345 10 11 558 6 579 9.12e-106 329
Msa0539700 MtrunA17_Chr2g0284701 33.841 591 352 10 2 555 4 592 2.91e-105 328
Msa0539700 MtrunA17_Chr1g0166731 39.474 456 251 8 116 550 9 460 5.78e-105 323
Msa0539700 MtrunA17_Chr1g0213711 34.672 548 320 10 27 548 19 554 4.92e-104 325
Msa0539700 MtrunA17_Chr7g0261661 37.281 456 262 6 117 551 1 453 7.01e-103 319
Msa0539700 MtrunA17_Chr4g0059971 35.401 548 322 9 35 552 40 585 2.33e-102 321
Msa0539700 MtrunA17_Chr8g0362711 34.386 570 333 8 18 554 13 574 7.02e-102 319
Msa0539700 MtrunA17_Chr7g0218851 34.698 562 331 13 23 557 40 592 2.92e-101 318
Msa0539700 MtrunA17_Chr7g0261751 36.722 482 245 8 17 441 19 497 5.38e-101 315
Msa0539700 MtrunA17_Chr2g0276751 34.851 571 332 11 10 558 19 571 6.25e-101 317
Msa0539700 MtrunA17_Chr8g0362781 33.625 571 338 11 23 559 24 587 1.05e-100 316
Msa0539700 MtrunA17_Chr3g0133341 34.182 550 330 9 25 551 10 550 3.21e-100 314
Msa0539700 MtrunA17_Chr5g0439381 37.030 559 310 10 17 551 16 556 1.45e-98 311
Msa0539700 MtrunA17_Chr8g0390331 34.058 552 331 11 23 548 9 553 2.04e-98 310
Msa0539700 MtrunA17_Chr4g0059991 33.880 549 326 10 35 550 28 572 5.00e-98 310
Msa0539700 MtrunA17_Chr1g0147631 32.982 570 342 12 4 548 10 564 6.95e-97 306
Msa0539700 MtrunA17_Chr8g0379971 33.445 598 341 13 15 559 6 599 1.13e-96 306
Msa0539700 MtrunA17_Chr1g0157611 33.748 563 334 10 16 548 1 554 7.39e-95 300
Msa0539700 MtrunA17_Chr4g0011041 32.860 563 339 12 17 548 24 578 2.82e-94 299
Msa0539700 MtrunA17_Chr4g0059981 33.737 578 340 11 6 552 17 582 1.25e-93 298
Msa0539700 MtrunA17_Chr4g0003761 33.394 551 334 10 25 550 27 569 1.58e-93 298
Msa0539700 MtrunA17_Chr7g0214731 31.777 557 337 9 30 550 62 611 2.67e-93 298
Msa0539700 MtrunA17_Chr1g0157601 32.264 561 344 11 16 548 1 553 5.79e-92 293
Msa0539700 MtrunA17_Chr2g0331521 31.204 548 343 10 29 548 22 563 3.06e-90 289
Msa0539700 MtrunA17_Chr4g0060001 32.194 556 340 8 27 553 27 574 2.14e-89 287
Msa0539700 MtrunA17_Chr2g0331471 33.741 572 338 16 10 550 68 629 9.07e-89 288
Msa0539700 MtrunA17_Chr4g0069581 35.113 487 283 10 87 552 44 518 8.35e-88 282
Msa0539700 MtrunA17_Chr7g0240271 30.142 564 356 9 17 549 12 568 9.97e-88 282
Msa0539700 MtrunA17_Chr1g0157621 32.571 525 320 9 50 548 1 517 1.03e-87 281
Msa0539700 MtrunA17_Chr2g0331481 32.286 573 346 14 10 550 4 566 1.11e-87 283
Msa0539700 MtrunA17_Chr1g0193391 32.194 556 348 13 17 553 3 548 7.68e-87 280
Msa0539700 MtrunA17_Chr4g0011471 31.818 528 323 9 50 548 37 556 3.43e-85 275
Msa0539700 MtrunA17_Chr2g0331511 30.634 568 356 12 10 548 4 562 4.58e-85 276
Msa0539700 MtrunA17_Chr1g0177591 32.423 549 336 13 19 550 34 564 5.24e-85 275
Msa0539700 MtrunA17_Chr8g0379991 34.159 565 308 12 58 559 9 572 6.25e-85 275
Msa0539700 MtrunA17_Chr7g0271221 32.288 542 340 10 29 553 17 548 1.46e-84 274
Msa0539700 MtrunA17_Chr2g0331461 31.469 572 352 13 10 550 4 566 2.82e-82 269
Msa0539700 MtrunA17_Chr7g0261731 40.361 332 175 5 17 327 19 348 2.77e-79 253
Msa0539700 MtrunA17_Chr8g0362801 34.389 442 266 7 133 557 31 465 2.17e-78 254
Msa0539700 MtrunA17_Chr7g0240281 30.755 556 345 9 27 549 22 570 5.09e-78 257
Msa0539700 MtrunA17_Chr3g0112631 42.222 270 150 3 287 551 4 272 7.37e-75 239
Msa0539700 MtrunA17_Chr5g0415841 32.326 430 271 6 137 551 35 459 1.88e-72 239
Msa0539700 MtrunA17_Chr2g0326211 31.818 572 331 15 16 552 1 548 1.06e-71 239
Msa0539700 MtrunA17_Chr2g0325951 30.648 571 339 14 16 552 1 548 1.52e-69 233
Msa0539700 MtrunA17_Chr2g0325961 31.763 573 331 15 16 552 1 549 5.63e-68 229
Msa0539700 MtrunA17_Chr1g0177611 31.486 451 276 12 117 550 1 435 3.91e-66 221
Msa0539700 MtrunA17_Chr7g0235521 30.269 446 281 8 127 549 19 457 3.90e-64 217
Msa0539700 MtrunA17_Chr7g0240131 30.045 446 282 8 127 549 19 457 7.28e-63 214
Msa0539700 MtrunA17_Chr7g0240211 30.045 446 282 8 127 549 19 457 9.15e-63 213
Msa0539700 MtrunA17_Chr7g0240181 30.045 446 282 8 127 549 19 457 9.64e-63 213
Msa0539700 MtrunA17_Chr1g0177631 30.290 449 282 11 117 548 1 435 2.96e-62 211
Msa0539700 MtrunA17_Chr4g0014851 81.897 116 20 1 1 115 1 116 3.93e-60 196
Msa0539700 MtrunA17_Chr3g0142111 42.481 266 142 4 9 265 26 289 4.09e-60 202
Msa0539700 MtrunA17_Chr4g0060071 38.007 271 163 4 282 551 32 298 1.99e-59 199
Msa0539700 MtrunA17_Chr7g0240151 28.795 448 267 9 127 549 19 439 5.29e-57 197
Msa0539700 MtrunA17_Chr3g0142121 38.636 264 144 7 287 545 12 262 1.67e-54 186
Msa0539700 MtrunA17_Chr7g0234181 29.655 435 279 8 119 548 1 413 9.68e-50 177
Msa0539700 MtrunA17_Chr7g0234151 29.841 439 279 8 116 548 3 418 2.22e-49 176
Msa0539700 MtrunA17_Chr7g0234381 28.929 439 283 8 116 548 3 418 1.55e-48 174
Msa0539700 MtrunA17_Chr7g0261721 39.899 198 118 1 354 551 5 201 1.09e-47 166
Msa0539700 MtrunA17_Chr3g0112651 38.312 154 84 3 116 261 21 171 1.47e-29 115
Msa0539700 MtrunA17_Chr1g0166721 51.515 99 48 0 17 115 13 111 3.69e-29 112
Msa0539700 MtrunA17_Chr3g0112661 40.517 116 68 1 17 132 19 133 1.10e-27 108
Msa0539700 MtrunA17_Chr7g0261671 45.455 99 54 0 17 115 20 118 1.35e-26 104
Msa0539700 MtrunA17_Chr1g0147591 30.303 198 122 7 141 324 3 198 4.22e-24 100
Msa0539700 MtrunA17_Chr3g0082621 41.111 90 53 0 44 133 1 90 2.94e-19 82.8
Msa0539700 MtrunA17_Chr2g0326221 26.087 230 140 3 322 551 24 223 2.76e-18 84.0
Msa0539700 MtrunA17_Chr4g0060061 36.275 102 65 0 137 238 15 116 4.66e-18 80.9
Msa0539700 MtrunA17_Chr7g0261831 30.726 179 97 5 251 407 3 176 5.28e-16 77.4
Msa0539700 MtrunA17_Chr1g0147581 35.398 113 65 2 414 525 11 116 7.08e-15 71.2
Msa0539700 MtrunA17_Chr7g0240221 28.283 99 71 0 17 115 15 113 2.01e-13 67.0
Msa0539700 MtrunA17_Chr7g0240191 28.283 99 71 0 17 115 15 113 2.01e-13 67.0
Msa0539700 MtrunA17_Chr7g0240161 28.283 99 71 0 17 115 15 113 2.01e-13 67.0
Msa0539700 MtrunA17_Chr7g0240141 27.273 99 72 0 17 115 15 113 7.98e-13 65.5
Msa0539700 MtrunA17_Chr4g0060051 32.039 103 70 0 10 112 3 105 6.85e-12 63.2
Msa0539700 MtrunA17_Chr7g0261821 41.791 67 39 0 489 555 1 67 2.16e-11 59.7
Msa0539700 MtrunA17_Chr7g0235511 26.733 101 72 1 17 115 15 115 5.38e-11 60.1
Msa0539700 MtrunA17_Chr8g0362791 36.364 77 49 0 35 111 42 118 9.86e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0539700 AT1G22540.1 64.376 553 185 2 10 555 9 556 0.0 689
Msa0539700 AT1G72140.1 56.466 549 211 5 16 551 19 552 0.0 618
Msa0539700 AT1G72125.1 58.029 548 223 5 12 552 10 557 0.0 616
Msa0539700 AT1G72120.1 57.500 560 223 8 1 552 1 553 0.0 606
Msa0539700 AT1G22550.1 55.477 566 230 6 1 552 3 560 0.0 596
Msa0539700 AT1G22570.1 55.851 564 221 7 10 552 5 561 0.0 583
Msa0539700 AT1G22570.2 56.061 528 208 6 42 552 4 524 0.0 550
Msa0539700 AT1G72130.1 53.458 535 221 8 29 555 23 537 0.0 540
Msa0539700 AT3G54450.1 44.177 541 275 7 29 551 11 542 1.25e-168 490
Msa0539700 AT5G01180.2 41.756 558 299 7 17 552 11 564 7.39e-155 455
Msa0539700 AT5G01180.3 41.756 558 299 7 17 552 11 564 7.39e-155 455
Msa0539700 AT5G01180.1 41.756 558 299 7 17 552 11 564 7.39e-155 455
Msa0539700 AT2G02040.1 41.084 572 309 8 1 548 11 578 2.56e-151 447
Msa0539700 AT3G54140.1 40.603 564 308 6 10 550 3 562 2.98e-151 446
Msa0539700 AT3G54140.2 40.603 564 308 6 10 550 3 562 2.98e-151 446
Msa0539700 AT1G72130.2 52.448 429 179 6 133 555 10 419 1.81e-149 436
Msa0539700 AT1G72130.3 52.448 429 179 6 133 555 10 419 1.81e-149 436
Msa0539700 AT1G62200.1 42.029 552 281 7 17 541 38 577 2.96e-148 439
Msa0539700 AT2G37900.1 40.493 568 294 11 10 551 17 566 5.37e-146 433
Msa0539700 AT3G53960.1 39.206 579 315 10 3 551 2 573 5.43e-145 431
Msa0539700 AT1G62200.2 40.792 581 276 11 17 541 38 606 1.58e-142 426
Msa0539700 AT1G62200.3 40.792 581 276 11 17 541 52 620 1.64e-142 426
Msa0539700 AT2G02020.1 40.853 563 290 12 1 550 10 542 2.44e-139 415
Msa0539700 AT2G40460.1 39.714 559 312 8 11 551 4 555 1.64e-137 411
Msa0539700 AT5G46050.1 38.571 560 319 6 17 555 13 568 5.09e-135 405
Msa0539700 AT5G46040.1 38.505 535 306 5 17 531 13 544 3.43e-133 400
Msa0539700 AT5G14940.1 40.000 545 291 11 29 548 5 538 7.12e-127 383
Msa0539700 AT2G02020.2 39.324 562 279 13 1 550 10 521 2.96e-125 378
Msa0539700 AT2G37900.2 40.755 503 256 11 74 551 2 487 2.69e-124 374
Msa0539700 AT3G01350.1 38.519 540 304 8 33 546 9 546 1.10e-118 362
Msa0539700 AT1G68570.1 38.192 542 300 8 40 551 32 568 1.78e-117 360
Msa0539700 AT1G68570.2 38.175 537 297 8 45 551 7 538 7.37e-117 358
Msa0539700 AT1G69870.1 37.251 553 312 11 28 551 48 594 9.77e-116 357
Msa0539700 AT1G12110.1 35.739 582 326 11 3 553 7 571 1.84e-111 344
Msa0539700 AT1G32450.1 35.727 571 330 9 10 548 16 581 6.61e-111 344
Msa0539700 AT2G26690.1 37.545 554 312 8 16 545 9 552 2.49e-108 336
Msa0539700 AT4G21680.1 35.764 576 335 12 5 550 2 572 5.00e-107 333
Msa0539700 AT3G21670.1 37.321 560 310 10 17 551 15 558 5.64e-104 325
Msa0539700 AT3G16180.1 34.526 559 322 12 25 548 16 565 9.33e-102 319
Msa0539700 AT1G27040.1 32.461 573 359 9 6 557 2 567 6.26e-100 314
Msa0539700 AT1G59740.1 34.326 571 338 11 3 546 12 572 1.04e-99 314
Msa0539700 AT1G27040.2 32.740 562 350 9 17 557 9 563 2.90e-99 312
Msa0539700 AT1G18880.1 33.938 551 323 10 35 551 23 566 5.61e-99 312
Msa0539700 AT5G46050.2 37.118 458 263 6 119 555 3 456 5.64e-99 308
Msa0539700 AT1G69850.1 32.137 585 355 9 9 555 3 583 5.98e-99 312
Msa0539700 AT1G33440.1 35.366 574 333 11 7 555 14 574 6.42e-99 312
Msa0539700 AT1G52190.1 33.889 540 312 11 40 544 30 559 1.39e-98 311
Msa0539700 AT2G26690.2 36.622 527 300 8 43 545 3 519 2.29e-97 306
Msa0539700 AT1G27040.3 33.022 536 331 9 43 557 7 535 4.16e-93 295
Msa0539700 AT2G26690.3 36.240 516 295 8 54 545 2 507 1.06e-92 294
Msa0539700 AT5G62680.1 32.486 551 333 10 35 551 47 592 8.19e-92 294
Msa0539700 AT5G62730.1 30.216 556 365 7 12 551 37 585 3.49e-91 292
Msa0539700 AT5G13400.1 31.794 563 350 8 20 551 52 611 4.88e-91 292
Msa0539700 AT3G47960.1 32.787 549 332 11 35 551 65 608 7.68e-90 290
Msa0539700 AT1G27080.1 32.496 557 332 11 29 556 12 553 6.85e-89 285
Msa0539700 AT5G19640.1 32.904 544 316 13 32 548 60 581 9.81e-89 286
Msa0539700 AT5G62730.2 30.132 531 348 7 37 551 3 526 2.16e-85 275
Msa0539700 AT1G69860.1 32.169 544 328 11 35 552 22 550 2.27e-83 270
Msa0539700 AT1G27080.2 32.443 524 310 11 62 556 3 511 4.20e-81 264
Msa0539700 AT5G28470.2 31.103 553 346 12 28 553 14 558 2.00e-80 263
Msa0539700 AT3G25280.1 32.897 535 328 11 21 548 11 521 3.90e-79 258
Msa0539700 AT3G25260.1 32.844 545 327 12 12 548 2 515 6.30e-78 255
Msa0539700 AT5G28470.1 30.922 553 345 13 28 553 14 556 8.64e-78 256
Msa0539700 AT3G45650.1 30.466 558 345 14 17 552 8 544 2.18e-69 233
Msa0539700 AT3G45680.1 30.634 568 342 13 11 552 2 543 6.85e-68 229
Msa0539700 AT3G45710.1 30.672 551 327 13 31 552 21 545 4.16e-66 225
Msa0539700 AT3G45700.1 30.203 543 335 10 30 552 15 533 7.61e-66 224
Msa0539700 AT3G45650.2 29.110 584 345 15 17 552 8 570 1.47e-63 219
Msa0539700 AT3G45660.1 30.268 522 313 13 57 552 47 543 2.79e-63 217
Msa0539700 AT3G45660.3 30.268 522 313 13 57 552 47 543 2.79e-63 217
Msa0539700 AT3G45660.2 30.268 522 313 13 57 552 68 564 4.45e-63 217
Msa0539700 AT5G11570.1 27.308 520 302 10 40 548 22 476 1.01e-62 214
Msa0539700 AT3G45720.1 29.870 539 327 14 40 553 28 540 6.77e-61 211
Msa0539700 AT3G45690.1 29.268 533 296 15 30 540 17 490 4.90e-53 189
Msa0539700 AT2G38100.2 27.925 530 316 17 59 558 2 495 3.58e-49 178
Msa0539700 AT2G38100.1 28.571 525 319 17 59 558 18 511 8.94e-49 177
Msa0539700 AT2G38100.3 28.571 525 319 17 59 558 24 517 1.04e-48 177

Find 118 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCATTCTTTCTTTATTA+AGG 0.160834 4_1:-20868372 None:intergenic
TGGGCTACAACTTTGGTTTA+TGG 0.163295 4_1:+20871276 Msa0539700:CDS
CTGCTGAAAATGTGAATATT+TGG 0.175954 4_1:+20869392 Msa0539700:CDS
AAACCTTGTGTTCAAGCTTT+TGG 0.192944 4_1:+20869754 Msa0539700:CDS
CTTCTAGCTTCATGCTCTTT+AGG 0.196981 4_1:-20869802 None:intergenic
TACTAAGGTTGGTTCCAATT+TGG 0.200350 4_1:+20871256 Msa0539700:CDS
CTAGAAGCTCATTCTTTAAT+TGG 0.206643 4_1:+20869818 Msa0539700:CDS
GATAACATTAGTTGGGTTCT+TGG 0.227701 4_1:+20869901 Msa0539700:CDS
CCCATGCTTCTTAGTTCATT+TGG 0.244157 4_1:-20871726 None:intergenic
ATTAGTTGGGTTCTTGGATT+TGG 0.259493 4_1:+20869907 Msa0539700:CDS
TTAGGTCCAAATCTGGTTAT+TGG 0.268204 4_1:+20868574 Msa0539700:CDS
TGCTTCTCTCATTTATATCT+TGG 0.273177 4_1:+20869495 Msa0539700:CDS
GACATATAGGTTTAACATTA+AGG 0.274752 4_1:+20869984 Msa0539700:CDS
GCTTCTCTACTTCCTCTCTT+TGG 0.279847 4_1:+20869424 Msa0539700:CDS
CCATGCTTCTTAGTTCATTT+GGG 0.297304 4_1:-20871725 None:intergenic
AGATCCGCTTGGTTCATAAT+TGG 0.298378 4_1:+20868597 Msa0539700:CDS
ACCAAAGTTGTAGCCCAAAT+TGG 0.299132 4_1:-20871270 None:intergenic
TCCAATTTGGGCTACAACTT+TGG 0.299380 4_1:+20871269 Msa0539700:CDS
ACTAAGGTTGGTTCCAATTT+GGG 0.304066 4_1:+20871257 Msa0539700:CDS
ACAAATATTCGGTCATAGAT+AGG 0.320728 4_1:-20871432 None:intergenic
TGACAATAGAGCAAGTTTAA+TGG 0.329336 4_1:+20872064 Msa0539700:three_prime_UTR
CTCATTGGAACAGTTGCATT+TGG 0.332167 4_1:-20871612 None:intergenic
AGGTTTAACATTAAGGATAA+TGG 0.338344 4_1:+20869991 Msa0539700:CDS
AAGAGTCCCTTTCTTAGAAT+TGG 0.345671 4_1:+20870015 Msa0539700:CDS
TTGTTGCGGCTGTAAGAAAT+TGG 0.346679 4_1:+20870046 Msa0539700:CDS
GCACTCTATTTGAGTATTGT+TGG 0.352342 4_1:+20871753 Msa0539700:CDS
AAAGAAGTGAATTATTGAAT+AGG 0.357324 4_1:+20868461 Msa0539700:five_prime_UTR
CATACAATCAATAACTTATC+TGG 0.357867 4_1:-20872135 None:intergenic
ATCACAATGCTTCAAAGAAT+TGG 0.365708 4_1:+20871492 Msa0539700:CDS
GCTCAATTAGCTCTTGTTGA+TGG 0.367128 4_1:+20868522 Msa0539700:CDS
GCAGAGAGGATATCATATTA+TGG 0.377037 4_1:+20869310 Msa0539700:CDS
CCAGATTTGGACCTAATTGC+GGG 0.377794 4_1:-20868567 None:intergenic
TCATCTTCTTTAGATCCCTT+TGG 0.381565 4_1:-20871195 None:intergenic
CTCCATTGATCTCCCTTGCT+TGG 0.381641 4_1:-20871331 None:intergenic
GACAATAGAGCAAGTTTAAT+GGG 0.392765 4_1:+20872065 Msa0539700:three_prime_UTR
CAACTGTTCCAATGAGTATA+TGG 0.398189 4_1:+20871619 Msa0539700:CDS
TTGCTAGAGGCACAAATATT+CGG 0.400786 4_1:-20871443 None:intergenic
CTTCGCTTCAATCGATTAAC+GGG 0.404016 4_1:+20871388 Msa0539700:CDS
GGATCTCCAATAACCAGATT+TGG 0.405683 4_1:-20868580 None:intergenic
ATGGAGAGAACAATAGTTGC+TGG 0.412347 4_1:+20871348 Msa0539700:CDS
ACAAAGGACAGCCCGCAATT+AGG 0.413326 4_1:+20868556 Msa0539700:CDS
TGAATTGTTCAGAACTTTGA+CGG 0.416306 4_1:-20870122 None:intergenic
GCTTCGCTTCAATCGATTAA+CGG 0.419179 4_1:+20871387 Msa0539700:CDS
TGCTGAAAATGTGAATATTT+GGG 0.419491 4_1:+20869393 Msa0539700:CDS
TGGTATGCATGAAATGATAA+TGG 0.433094 4_1:+20872040 Msa0539700:three_prime_UTR
TGCTTGGAACAAGGACATAT+AGG 0.436907 4_1:+20869971 Msa0539700:CDS
AACTTGATAACATATCTAAC+AGG 0.448274 4_1:+20869343 Msa0539700:CDS
TATTGAATAGGTTTCATTGA+TGG 0.453075 4_1:+20868473 Msa0539700:exon
CCCGCAATTAGGTCCAAATC+TGG 0.457824 4_1:+20868567 Msa0539700:CDS
CTTGCTGGATTAAGTGTGAT+GGG 0.459941 4_1:+20871900 Msa0539700:CDS
TGCTGGATTTGACATACCTC+CGG 0.461333 4_1:+20871365 Msa0539700:CDS
TGCTCTTAATATCATCGAAA+AGG 0.461967 4_1:+20872180 Msa0539700:three_prime_UTR
TTTACATTCTTTACCAAGCA+AGG 0.462198 4_1:+20871318 Msa0539700:CDS
ATATACAAGATAACATTAGT+TGG 0.471533 4_1:+20869893 Msa0539700:CDS
ATGTCCTTGTTCCAAGCAAA+AGG 0.472474 4_1:-20869966 None:intergenic
AAGAATGAGACGGGTGTTTG+TGG 0.474321 4_1:+20868384 Msa0539700:five_prime_UTR
GAAGCTCATTCTTTAATTGG+TGG 0.474451 4_1:+20869821 Msa0539700:CDS
TACACGACCAATTCTAAGAA+AGG 0.479274 4_1:-20870022 None:intergenic
GTGTTAGCAATGCCTTGTTA+AGG 0.482586 4_1:-20871173 None:intergenic
ACCAGCCACCATATACTCAT+TGG 0.483686 4_1:-20871627 None:intergenic
AGCTTCTAGCAACACTTCGT+TGG 0.490449 4_1:-20868502 None:intergenic
TATACAAGATAACATTAGTT+GGG 0.492477 4_1:+20869894 Msa0539700:CDS
GCAAAGATGGACATGATAGT+TGG 0.492895 4_1:+20871829 Msa0539700:CDS
GCTTGCTGGATTAAGTGTGA+TGG 0.498470 4_1:+20871899 Msa0539700:CDS
TAGCTTCTTCCACCTCACTA+AGG 0.507911 4_1:-20871227 None:intergenic
CAAATATTCGGTCATAGATA+GGG 0.518549 4_1:-20871431 None:intergenic
TCACTCTATCTAGTAGCCAT+TGG 0.518838 4_1:+20869718 Msa0539700:CDS
AAGGCATTGCTAACACCAAA+GGG 0.521029 4_1:+20871180 Msa0539700:CDS
AGCTAAGGCTGTACTAAGGT+TGG 0.525208 4_1:+20871245 Msa0539700:CDS
ACACGACCAATTCTAAGAAA+GGG 0.526584 4_1:-20870021 None:intergenic
TTGAAGCATTGTGATGCCAG+AGG 0.532437 4_1:-20871484 None:intergenic
GAAAATGTGAATATTTGGGC+TGG 0.534692 4_1:+20869397 Msa0539700:CDS
GGGCTGTCCTTTGTAGTCAA+CGG 0.537218 4_1:-20868547 None:intergenic
CATTGGCAGCTTCCTTAACA+AGG 0.537278 4_1:+20871161 Msa0539700:intron
TAGTGAGGTGGAAGAAGCTA+AGG 0.539918 4_1:+20871230 Msa0539700:CDS
TCCAATGAGTATATGGTGGC+TGG 0.541211 4_1:+20871626 Msa0539700:CDS
CCCAAATGAACTAAGAAGCA+TGG 0.542573 4_1:+20871725 Msa0539700:CDS
TTTGCAGTGCTCTGGTTGAG+TGG 0.543870 4_1:-20869367 None:intergenic
TATTTGAGTATTGTTGGTGT+TGG 0.547399 4_1:+20871759 Msa0539700:CDS
GTTATCACCGGAAAACCCTC+TGG 0.549167 4_1:+20871468 Msa0539700:CDS
GCGAAATAACTTATCAACGA+TGG 0.551064 4_1:+20870068 Msa0539700:CDS
GCAATGATCATTACAACGCA+AGG 0.553402 4_1:-20869934 None:intergenic
TAATATGAAATACAGGGACT+AGG 0.554304 4_1:+20869619 Msa0539700:intron
ATGCTTCAAAGAATTGGCAC+TGG 0.559271 4_1:+20871498 Msa0539700:CDS
GCCCCAAAAGCTTGAACACA+AGG 0.560276 4_1:-20869757 None:intergenic
ACCAGATTTGGACCTAATTG+CGG 0.560532 4_1:-20868568 None:intergenic
CTGGTTATTGGAGATCCGCT+TGG 0.561853 4_1:+20868586 Msa0539700:CDS
TAATAAAGAAAGAATGAGAC+GGG 0.563912 4_1:+20868375 Msa0539700:five_prime_UTR
TTAATCGATTGAAGCGAAGC+CGG 0.566971 4_1:-20871384 None:intergenic
TGAAGCATTGTGATGCCAGA+GGG 0.574064 4_1:-20871483 None:intergenic
TTTATGTCCACCTTGTCCAA+TGG 0.577427 4_1:-20869734 None:intergenic
CAAGGCATTGCTAACACCAA+AGG 0.579415 4_1:+20871179 Msa0539700:CDS
CCAAATGAACTAAGAAGCAT+GGG 0.579849 4_1:+20871726 Msa0539700:CDS
TTACATTCTTTACCAAGCAA+GGG 0.580852 4_1:+20871319 Msa0539700:CDS
GCAACGAAAGCACCAAAGAG+AGG 0.585368 4_1:-20869436 None:intergenic
AAGAAGCTAAGGCTGTACTA+AGG 0.586827 4_1:+20871241 Msa0539700:CDS
AACTTGTAGCCTTAGTGAGG+TGG 0.589145 4_1:+20871218 Msa0539700:CDS
TACCAAGCAAGGGAGATCAA+TGG 0.593347 4_1:+20871329 Msa0539700:CDS
AATTGGTCGTGTATTTGTTG+CGG 0.594432 4_1:+20870032 Msa0539700:CDS
CACAAAGGCACTATCACACA+AGG 0.599412 4_1:+20871966 Msa0539700:CDS
TTAATAAAGAAAGAATGAGA+CGG 0.612886 4_1:+20868374 Msa0539700:intron
CAATGATCATTACAACGCAA+GGG 0.615247 4_1:-20869933 None:intergenic
CCGGTGATAACTCTTGCTAG+AGG 0.619219 4_1:-20871456 None:intergenic
GAAGAACAGAATGTGAGGGA+AGG 0.619249 4_1:-20869667 None:intergenic
ACTCAGAAGAACAGAATGTG+AGG 0.623759 4_1:-20869672 None:intergenic
TATCTAGTAGCCATTGGACA+AGG 0.631063 4_1:+20869724 Msa0539700:CDS
ATCGATTGAAGCGAAGCCGG+AGG 0.634016 4_1:-20871381 None:intergenic
CCTCTAGCAAGAGTTATCAC+CGG 0.634596 4_1:+20871456 Msa0539700:CDS
TTGCTGGATTAAGTGTGATG+GGG 0.634633 4_1:+20871901 Msa0539700:CDS
TAGGTGTGGAAGTGGCAGAG+AGG 0.644513 4_1:+20869296 Msa0539700:intron
TCTTACATTTATAATCACAA+AGG 0.648613 4_1:+20871951 Msa0539700:CDS
CTCAGAAGAACAGAATGTGA+GGG 0.652478 4_1:-20869671 None:intergenic
CTAGTAGCCATTGGACAAGG+TGG 0.655661 4_1:+20869727 Msa0539700:CDS
TGAAACTTGTAGCCTTAGTG+AGG 0.657268 4_1:+20871215 Msa0539700:CDS
GATGGCGCCGTTGACTACAA+AGG 0.674675 4_1:+20868540 Msa0539700:CDS
GAGAAGCAAGTATGATTGTG+CGG 0.679159 4_1:-20869480 None:intergenic
GATACCAATTATGAACCAAG+CGG 0.690664 4_1:-20868601 None:intergenic
CTGTTCCAATGAGTATATGG+TGG 0.717004 4_1:+20871622 Msa0539700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGAAATATTAGCAAAAT+TGG + chr4_1:20868865-20868884 Msa0539700:intron 15.0%
!!! TTTGAAATATTAGCAAAATT+GGG + chr4_1:20868866-20868885 Msa0539700:intron 15.0%
!!! TAATTATTTTAAACGTTATG+AGG + chr4_1:20869246-20869265 Msa0539700:intron 15.0%
!! CAATTATAGTACATTATTAA+TGG + chr4_1:20870871-20870890 Msa0539700:intron 15.0%
!! CAAAGGAAAAAAAAAAAAAT+TGG + chr4_1:20870915-20870934 Msa0539700:intron 15.0%
!! TGTTATGATAACTATATAAA+AGG - chr4_1:20871004-20871023 None:intergenic 15.0%
!!! AAAATAGTCATTATTTTTCA+AGG - chr4_1:20871121-20871140 None:intergenic 15.0%
!!! AATTTTTATATCGATATTCA+CGG + chr4_1:20871521-20871540 Msa0539700:CDS 15.0%
!! TTAATAAAGAAAGAATGAGA+CGG + chr4_1:20868374-20868393 Msa0539700:intron 20.0%
!! AAAGAAGTGAATTATTGAAT+AGG + chr4_1:20868461-20868480 Msa0539700:five_prime_UTR 20.0%
!! AGATTGAAATATTGAATAGT+CGG - chr4_1:20868781-20868800 None:intergenic 20.0%
!! TTGAAATATTAGCAAAATTG+GGG + chr4_1:20868867-20868886 Msa0539700:intron 20.0%
!! ATGGATCAATTATTAAATCT+TGG + chr4_1:20868904-20868923 Msa0539700:intron 20.0%
!! ATCAATTATTAAATCTTGGA+TGG + chr4_1:20868908-20868927 Msa0539700:intron 20.0%
!! TCAACAAAAATTGATGTATT+TGG - chr4_1:20869178-20869197 None:intergenic 20.0%
!! TCAATTGTCTCTTATATTTA+AGG + chr4_1:20869199-20869218 Msa0539700:intron 20.0%
!!! TTTTTTTCTTCTGTTCATAT+TGG + chr4_1:20869541-20869560 Msa0539700:intron 20.0%
!! ATTGGATTAATATGAAATAC+AGG + chr4_1:20869612-20869631 Msa0539700:intron 20.0%
!! TTGGATTAATATGAAATACA+GGG + chr4_1:20869613-20869632 Msa0539700:intron 20.0%
!! ATATACAAGATAACATTAGT+TGG + chr4_1:20869893-20869912 Msa0539700:CDS 20.0%
!! TATACAAGATAACATTAGTT+GGG + chr4_1:20869894-20869913 Msa0539700:CDS 20.0%
!!! ACAAATTGATTTGAAATGAA+TGG - chr4_1:20870188-20870207 None:intergenic 20.0%
!!! AATCAAATCCATTGATTATA+TGG - chr4_1:20870369-20870388 None:intergenic 20.0%
!! ATCTGCTTATTTCAATTTAT+TGG + chr4_1:20870840-20870859 Msa0539700:intron 20.0%
!! AATTTGATTACATTGAGTAT+TGG + chr4_1:20870975-20870994 Msa0539700:intron 20.0%
!! CAATTAATATTATCATTGAC+CGG + chr4_1:20871048-20871067 Msa0539700:intron 20.0%
!! TTTACTTTGTTAGAATTAGT+TGG - chr4_1:20871097-20871116 None:intergenic 20.0%
!!! TCTTTGATAGAAAATTTTAG+TGG + chr4_1:20871807-20871826 Msa0539700:CDS 20.0%
!!! CACATTTTGATTACTTTTAT+TGG + chr4_1:20871874-20871893 Msa0539700:CDS 20.0%
!! TCTTACATTTATAATCACAA+AGG + chr4_1:20871951-20871970 Msa0539700:CDS 20.0%
! GTCTCATTCTTTCTTTATTA+AGG - chr4_1:20868375-20868394 None:intergenic 25.0%
! TAATAAAGAAAGAATGAGAC+GGG + chr4_1:20868375-20868394 Msa0539700:five_prime_UTR 25.0%
! TATTGAATAGGTTTCATTGA+TGG + chr4_1:20868473-20868492 Msa0539700:exon 25.0%
!!! TTTCTTTTACCTATTTCTCT+CGG + chr4_1:20868934-20868953 Msa0539700:intron 25.0%
! ATATGTTCCTTATGTTAACT+CGG + chr4_1:20869077-20869096 Msa0539700:intron 25.0%
! AAATAAGTAAACCACCAAAT+TGG - chr4_1:20869150-20869169 None:intergenic 25.0%
!! GAGTAAAGAATGTTTTGTAT+AGG + chr4_1:20869277-20869296 Msa0539700:intron 25.0%
!! AACTTGATAACATATCTAAC+AGG + chr4_1:20869343-20869362 Msa0539700:CDS 25.0%
! TGCTGAAAATGTGAATATTT+GGG + chr4_1:20869393-20869412 Msa0539700:CDS 25.0%
!!! TTGGTTTATTGTTTTGATGT+TGG + chr4_1:20869587-20869606 Msa0539700:intron 25.0%
!!! ATTGTTTTGATGTTGGTAAT+TGG + chr4_1:20869594-20869613 Msa0539700:intron 25.0%
! GACATATAGGTTTAACATTA+AGG + chr4_1:20869984-20870003 Msa0539700:CDS 25.0%
! AGGTTTAACATTAAGGATAA+TGG + chr4_1:20869991-20870010 Msa0539700:CDS 25.0%
! CTTCTTATCCATATAATCAA+TGG + chr4_1:20870358-20870377 Msa0539700:intron 25.0%
! TCTCTTGGTTATAAATAACA+CGG + chr4_1:20870393-20870412 Msa0539700:intron 25.0%
! TAAAGCAATTTACATAGAAG+AGG - chr4_1:20870760-20870779 None:intergenic 25.0%
!!! TTAGCTTGTAATTTCTACAT+TGG + chr4_1:20871144-20871163 Msa0539700:intron 25.0%
!!! ATTCCAAACAAAAAGTATTG+AGG - chr4_1:20871654-20871673 None:intergenic 25.0%
!!! AATTTCTGAGGTTTTTACTA+TGG + chr4_1:20871671-20871690 Msa0539700:CDS 25.0%
!! GTTTTGTGACAATATCAATA+AGG + chr4_1:20871851-20871870 Msa0539700:CDS 25.0%
! CATACAATCAATAACTTATC+TGG - chr4_1:20872138-20872157 None:intergenic 25.0%
!!! TGTGTTTTTGATTTTCTTGC+TGG + chr4_1:20868706-20868725 Msa0539700:intron 30.0%
AATAGATTACCGAGAGAAAT+AGG - chr4_1:20868946-20868965 None:intergenic 30.0%
TTAACTCGGTTCATGAATTA+TGG + chr4_1:20869091-20869110 Msa0539700:intron 30.0%
! TTGTCTCTTATATTTAAGGC+AGG + chr4_1:20869203-20869222 Msa0539700:intron 30.0%
! AAGAATGTTTTGTATAGGTG+TGG + chr4_1:20869282-20869301 Msa0539700:intron 30.0%
CTGCTGAAAATGTGAATATT+TGG + chr4_1:20869392-20869411 Msa0539700:CDS 30.0%
TGCTTCTCTCATTTATATCT+TGG + chr4_1:20869495-20869514 Msa0539700:CDS 30.0%
TAATATGAAATACAGGGACT+AGG + chr4_1:20869619-20869638 Msa0539700:intron 30.0%
CTAGAAGCTCATTCTTTAAT+TGG + chr4_1:20869818-20869837 Msa0539700:CDS 30.0%
! TGGTATTTTACTATGATTGC+AGG + chr4_1:20869841-20869860 Msa0539700:CDS 30.0%
!! GATTTTGAAGAAGAATGTGA+TGG + chr4_1:20870090-20870109 Msa0539700:CDS 30.0%
TGAATTGTTCAGAACTTTGA+CGG - chr4_1:20870125-20870144 None:intergenic 30.0%
AAAAACAAGTGTGTTTGGTT+CGG - chr4_1:20870218-20870237 None:intergenic 30.0%
CATTGAAAAACAAGTGTGTT+TGG - chr4_1:20870223-20870242 None:intergenic 30.0%
! ATTATATGGATAAGAAGCAC+TGG - chr4_1:20870355-20870374 None:intergenic 30.0%
TGGATTTGATTGTAATCTCT+TGG + chr4_1:20870378-20870397 Msa0539700:intron 30.0%
TAGCTTCTTTATTCTTCTCT+GGG + chr4_1:20870504-20870523 Msa0539700:intron 30.0%
!! GCGTAGTTATTTCTTTGAAA+AGG + chr4_1:20870541-20870560 Msa0539700:intron 30.0%
! GCTTTGAGTAGAATAATACT+TGG + chr4_1:20870634-20870653 Msa0539700:intron 30.0%
TGGCAGAAAGATTGATTATT+TGG + chr4_1:20870935-20870954 Msa0539700:intron 30.0%
GGTTATATGTAGTTATAAGC+CGG - chr4_1:20871070-20871089 None:intergenic 30.0%
TTGTTAGAATTAGTTGGAGT+TGG - chr4_1:20871091-20871110 None:intergenic 30.0%
TTTACATTCTTTACCAAGCA+AGG + chr4_1:20871318-20871337 Msa0539700:CDS 30.0%
TTACATTCTTTACCAAGCAA+GGG + chr4_1:20871319-20871338 Msa0539700:CDS 30.0%
CAAATATTCGGTCATAGATA+GGG - chr4_1:20871434-20871453 None:intergenic 30.0%
ACAAATATTCGGTCATAGAT+AGG - chr4_1:20871435-20871454 None:intergenic 30.0%
ATCACAATGCTTCAAAGAAT+TGG + chr4_1:20871492-20871511 Msa0539700:CDS 30.0%
! TAGTCTTTTCATCTCAACAA+AGG - chr4_1:20871559-20871578 None:intergenic 30.0%
CTAAAAATAGCACAAGATCA+TGG + chr4_1:20871576-20871595 Msa0539700:CDS 30.0%
!! GTTCCTCAATACTTTTTGTT+TGG + chr4_1:20871648-20871667 Msa0539700:CDS 30.0%
!! CTTTTTGTTTGGAATTTCTG+AGG + chr4_1:20871659-20871678 Msa0539700:CDS 30.0%
! TATTTGAGTATTGTTGGTGT+TGG + chr4_1:20871759-20871778 Msa0539700:CDS 30.0%
!! GAAAATTTTAGTGGCAAAGA+TGG + chr4_1:20871816-20871835 Msa0539700:CDS 30.0%
TGGTATGCATGAAATGATAA+TGG + chr4_1:20872040-20872059 Msa0539700:three_prime_UTR 30.0%
TGACAATAGAGCAAGTTTAA+TGG + chr4_1:20872064-20872083 Msa0539700:three_prime_UTR 30.0%
GACAATAGAGCAAGTTTAAT+GGG + chr4_1:20872065-20872084 Msa0539700:three_prime_UTR 30.0%
TGCTCTTAATATCATCGAAA+AGG + chr4_1:20872180-20872199 Msa0539700:three_prime_UTR 30.0%
TTAGGTCCAAATCTGGTTAT+TGG + chr4_1:20868574-20868593 Msa0539700:CDS 35.0%
GATACCAATTATGAACCAAG+CGG - chr4_1:20868604-20868623 None:intergenic 35.0%
TTATAGAGACATGATCAGTG+TGG - chr4_1:20868739-20868758 None:intergenic 35.0%
TTTCAATCTCTGTCACATGA+CGG + chr4_1:20868792-20868811 Msa0539700:intron 35.0%
! CTTCGTCTCAATTGTTTTCA+CGG + chr4_1:20868974-20868993 Msa0539700:intron 35.0%
TCATGAACCGAGTTAACATA+AGG - chr4_1:20869087-20869106 None:intergenic 35.0%
GCAGAGAGGATATCATATTA+TGG + chr4_1:20869310-20869329 Msa0539700:CDS 35.0%
GAAAATGTGAATATTTGGGC+TGG + chr4_1:20869397-20869416 Msa0539700:CDS 35.0%
!!! TTCGTTGCTGATTCTTTTCT+TGG + chr4_1:20869451-20869470 Msa0539700:CDS 35.0%
! AAACCTTGTGTTCAAGCTTT+TGG + chr4_1:20869754-20869773 Msa0539700:CDS 35.0%
! AACCTTGTGTTCAAGCTTTT+GGG + chr4_1:20869755-20869774 Msa0539700:CDS 35.0%
GAAGCTCATTCTTTAATTGG+TGG + chr4_1:20869821-20869840 Msa0539700:CDS 35.0%
GATAACATTAGTTGGGTTCT+TGG + chr4_1:20869901-20869920 Msa0539700:CDS 35.0%
ATTAGTTGGGTTCTTGGATT+TGG + chr4_1:20869907-20869926 Msa0539700:CDS 35.0%
CAATGATCATTACAACGCAA+GGG - chr4_1:20869936-20869955 None:intergenic 35.0%
AAGAGTCCCTTTCTTAGAAT+TGG + chr4_1:20870015-20870034 Msa0539700:CDS 35.0%
! ACACGACCAATTCTAAGAAA+GGG - chr4_1:20870024-20870043 None:intergenic 35.0%
! TACACGACCAATTCTAAGAA+AGG - chr4_1:20870025-20870044 None:intergenic 35.0%
! AATTGGTCGTGTATTTGTTG+CGG + chr4_1:20870032-20870051 Msa0539700:CDS 35.0%
GCGAAATAACTTATCAACGA+TGG + chr4_1:20870068-20870087 Msa0539700:CDS 35.0%
!!! TAAGAAGCACTGGTTTTATC+CGG - chr4_1:20870345-20870364 None:intergenic 35.0%
CAGAGAAGAATAAAGAAGCT+AGG - chr4_1:20870505-20870524 None:intergenic 35.0%
CTAGCTTCTTTATTCTTCTC+TGG + chr4_1:20870503-20870522 Msa0539700:intron 35.0%
AATCGTTATTGTGACTTGAG+AGG + chr4_1:20870612-20870631 Msa0539700:intron 35.0%
! GAGTAGAATAATACTTGGTG+AGG + chr4_1:20870639-20870658 Msa0539700:intron 35.0%
TTGGTGAGGTTGTAAATTTG+AGG + chr4_1:20870653-20870672 Msa0539700:intron 35.0%
GAGGTTGTAAATTTGAGGAA+AGG + chr4_1:20870658-20870677 Msa0539700:intron 35.0%
AAGAAGGGAGTTGAATTATC+TGG - chr4_1:20870714-20870733 None:intergenic 35.0%
ACCACGTCGAAATATAAGAA+GGG - chr4_1:20870729-20870748 None:intergenic 35.0%
AACCACGTCGAAATATAAGA+AGG - chr4_1:20870730-20870749 None:intergenic 35.0%
! TTTAGAGTGCAGTTAGTGTT+TGG + chr4_1:20870776-20870795 Msa0539700:intron 35.0%
CATTATTAATGGCTCATCGT+AGG + chr4_1:20870882-20870901 Msa0539700:intron 35.0%
TCATCTTCTTTAGATCCCTT+TGG - chr4_1:20871198-20871217 None:intergenic 35.0%
! TACTAAGGTTGGTTCCAATT+TGG + chr4_1:20871256-20871275 Msa0539700:CDS 35.0%
! ACTAAGGTTGGTTCCAATTT+GGG + chr4_1:20871257-20871276 Msa0539700:CDS 35.0%
! TTGCTAGAGGCACAAATATT+CGG - chr4_1:20871446-20871465 None:intergenic 35.0%
CAACTGTTCCAATGAGTATA+TGG + chr4_1:20871619-20871638 Msa0539700:CDS 35.0%
!! TCTGAGGTTTTTACTATGGT+TGG + chr4_1:20871675-20871694 Msa0539700:CDS 35.0%
!!! TTTTACTATGGTTGGTCTTC+AGG + chr4_1:20871683-20871702 Msa0539700:CDS 35.0%
CCATGCTTCTTAGTTCATTT+GGG - chr4_1:20871728-20871747 None:intergenic 35.0%
! CCAAATGAACTAAGAAGCAT+GGG + chr4_1:20871726-20871745 Msa0539700:CDS 35.0%
! GCACTCTATTTGAGTATTGT+TGG + chr4_1:20871753-20871772 Msa0539700:CDS 35.0%
!! TACTTTTATTGGTTGCTTGC+TGG + chr4_1:20871885-20871904 Msa0539700:CDS 35.0%
! GCTCAATTAGCTCTTGTTGA+TGG + chr4_1:20868522-20868541 Msa0539700:CDS 40.0%
ACCAGATTTGGACCTAATTG+CGG - chr4_1:20868571-20868590 None:intergenic 40.0%
GGATCTCCAATAACCAGATT+TGG - chr4_1:20868583-20868602 None:intergenic 40.0%
AGATCCGCTTGGTTCATAAT+TGG + chr4_1:20868597-20868616 Msa0539700:CDS 40.0%
GTCGGAGACTAATCTAAAAG+TGG - chr4_1:20868763-20868782 None:intergenic 40.0%
TGGGGACTAATTTGAGAGAA+TGG + chr4_1:20868885-20868904 Msa0539700:intron 40.0%
GCAAGAGATAAGGACCAATT+TGG + chr4_1:20869133-20869152 Msa0539700:intron 40.0%
AGAGATAAGGACCAATTTGG+TGG + chr4_1:20869136-20869155 Msa0539700:intron 40.0%
! GTTTTGTATAGGTGTGGAAG+TGG + chr4_1:20869288-20869307 Msa0539700:intron 40.0%
GAGAAGCAAGTATGATTGTG+CGG - chr4_1:20869483-20869502 None:intergenic 40.0%
! AGATAGACAGATAGCTCTGT+TGG + chr4_1:20869568-20869587 Msa0539700:intron 40.0%
CTCAGAAGAACAGAATGTGA+GGG - chr4_1:20869674-20869693 None:intergenic 40.0%
ACTCAGAAGAACAGAATGTG+AGG - chr4_1:20869675-20869694 None:intergenic 40.0%
TCACTCTATCTAGTAGCCAT+TGG + chr4_1:20869718-20869737 Msa0539700:CDS 40.0%
TATCTAGTAGCCATTGGACA+AGG + chr4_1:20869724-20869743 Msa0539700:CDS 40.0%
TTTATGTCCACCTTGTCCAA+TGG - chr4_1:20869737-20869756 None:intergenic 40.0%
! ACCTTGTGTTCAAGCTTTTG+GGG + chr4_1:20869756-20869775 Msa0539700:CDS 40.0%
!! CTTCTAGCTTCATGCTCTTT+AGG - chr4_1:20869805-20869824 None:intergenic 40.0%
GCAATGATCATTACAACGCA+AGG - chr4_1:20869937-20869956 None:intergenic 40.0%
ATGTCCTTGTTCCAAGCAAA+AGG - chr4_1:20869969-20869988 None:intergenic 40.0%
TGCTTGGAACAAGGACATAT+AGG + chr4_1:20869971-20869990 Msa0539700:CDS 40.0%
TTGTTGCGGCTGTAAGAAAT+TGG + chr4_1:20870046-20870065 Msa0539700:CDS 40.0%
GAAACCACTATGATTTCACC+CGG + chr4_1:20870323-20870342 Msa0539700:intron 40.0%
TTATCCGGGTGAAATCATAG+TGG - chr4_1:20870330-20870349 None:intergenic 40.0%
!!! AAGAAGCACTGGTTTTATCC+GGG - chr4_1:20870344-20870363 None:intergenic 40.0%
TCCCTTCTTATATTTCGACG+TGG + chr4_1:20870725-20870744 Msa0539700:intron 40.0%
TCGTAGGTAAAGCTCAACAA+AGG + chr4_1:20870898-20870917 Msa0539700:intron 40.0%
! GTGTTAGCAATGCCTTGTTA+AGG - chr4_1:20871176-20871195 None:intergenic 40.0%
!! AAGGCATTGCTAACACCAAA+GGG + chr4_1:20871180-20871199 Msa0539700:CDS 40.0%
! TGAAACTTGTAGCCTTAGTG+AGG + chr4_1:20871215-20871234 Msa0539700:CDS 40.0%
AAGAAGCTAAGGCTGTACTA+AGG + chr4_1:20871241-20871260 Msa0539700:CDS 40.0%
TCCAATTTGGGCTACAACTT+TGG + chr4_1:20871269-20871288 Msa0539700:CDS 40.0%
ACCAAAGTTGTAGCCCAAAT+TGG - chr4_1:20871273-20871292 None:intergenic 40.0%
! TGGGCTACAACTTTGGTTTA+TGG + chr4_1:20871276-20871295 Msa0539700:CDS 40.0%
ATGGAGAGAACAATAGTTGC+TGG + chr4_1:20871348-20871367 Msa0539700:CDS 40.0%
TTAATCGATTGAAGCGAAGC+CGG - chr4_1:20871387-20871406 None:intergenic 40.0%
GCTTCGCTTCAATCGATTAA+CGG + chr4_1:20871387-20871406 Msa0539700:CDS 40.0%
CTTCGCTTCAATCGATTAAC+GGG + chr4_1:20871388-20871407 Msa0539700:CDS 40.0%
ATGCTTCAAAGAATTGGCAC+TGG + chr4_1:20871498-20871517 Msa0539700:CDS 40.0%
CTCATTGGAACAGTTGCATT+TGG - chr4_1:20871615-20871634 None:intergenic 40.0%
CTGTTCCAATGAGTATATGG+TGG + chr4_1:20871622-20871641 Msa0539700:CDS 40.0%
CCCAAATGAACTAAGAAGCA+TGG + chr4_1:20871725-20871744 Msa0539700:CDS 40.0%
CCCATGCTTCTTAGTTCATT+TGG - chr4_1:20871729-20871748 None:intergenic 40.0%
!! GGTGTTGGAAGCTTTTTAAG+TGG + chr4_1:20871774-20871793 Msa0539700:CDS 40.0%
GCAAAGATGGACATGATAGT+TGG + chr4_1:20871829-20871848 Msa0539700:CDS 40.0%
CTTGCTGGATTAAGTGTGAT+GGG + chr4_1:20871900-20871919 Msa0539700:CDS 40.0%
TTGCTGGATTAAGTGTGATG+GGG + chr4_1:20871901-20871920 Msa0539700:CDS 40.0%
! TTTTGTGCAGAAGAGCAGTT+TGG + chr4_1:20872020-20872039 Msa0539700:three_prime_UTR 40.0%
AAGAATGAGACGGGTGTTTG+TGG + chr4_1:20868384-20868403 Msa0539700:five_prime_UTR 45.0%
AGCTTCTAGCAACACTTCGT+TGG - chr4_1:20868505-20868524 None:intergenic 45.0%
CCAGATTTGGACCTAATTGC+GGG - chr4_1:20868570-20868589 None:intergenic 45.0%
TAGAGACATGATCAGTGTGG+TGG - chr4_1:20868736-20868755 None:intergenic 45.0%
!! TCACATGACGGATACTACTG+AGG + chr4_1:20868804-20868823 Msa0539700:intron 45.0%
!! CACATGACGGATACTACTGA+GGG + chr4_1:20868805-20868824 Msa0539700:intron 45.0%
!! ACATGACGGATACTACTGAG+GGG + chr4_1:20868806-20868825 Msa0539700:intron 45.0%
GCTTCTCTACTTCCTCTCTT+TGG + chr4_1:20869424-20869443 Msa0539700:CDS 45.0%
GAAGAACAGAATGTGAGGGA+AGG - chr4_1:20869670-20869689 None:intergenic 45.0%
!! TTGTGTTCAAGCTTTTGGGG+CGG + chr4_1:20869759-20869778 Msa0539700:CDS 45.0%
! GCTTTGACTGTCCTTTTGCT+TGG + chr4_1:20869955-20869974 Msa0539700:CDS 45.0%
! CTGTCCTTTTGCTTGGAACA+AGG + chr4_1:20869962-20869981 Msa0539700:CDS 45.0%
!! AACAAGTGTGTTTGGTTCGG+TGG - chr4_1:20870215-20870234 None:intergenic 45.0%
CATTGGCAGCTTCCTTAACA+AGG + chr4_1:20871161-20871180 Msa0539700:intron 45.0%
!! CAAGGCATTGCTAACACCAA+AGG + chr4_1:20871179-20871198 Msa0539700:CDS 45.0%
! AACTTGTAGCCTTAGTGAGG+TGG + chr4_1:20871218-20871237 Msa0539700:CDS 45.0%
TAGCTTCTTCCACCTCACTA+AGG - chr4_1:20871230-20871249 None:intergenic 45.0%
TAGTGAGGTGGAAGAAGCTA+AGG + chr4_1:20871230-20871249 Msa0539700:CDS 45.0%
AGCTAAGGCTGTACTAAGGT+TGG + chr4_1:20871245-20871264 Msa0539700:CDS 45.0%
TACCAAGCAAGGGAGATCAA+TGG + chr4_1:20871329-20871348 Msa0539700:CDS 45.0%
TGCTGGATTTGACATACCTC+CGG + chr4_1:20871365-20871384 Msa0539700:CDS 45.0%
CCTCTAGCAAGAGTTATCAC+CGG + chr4_1:20871456-20871475 Msa0539700:CDS 45.0%
!! TGAAGCATTGTGATGCCAGA+GGG - chr4_1:20871486-20871505 None:intergenic 45.0%
!! TTGAAGCATTGTGATGCCAG+AGG - chr4_1:20871487-20871506 None:intergenic 45.0%
TCCAATGAGTATATGGTGGC+TGG + chr4_1:20871626-20871645 Msa0539700:CDS 45.0%
ACCAGCCACCATATACTCAT+TGG - chr4_1:20871630-20871649 None:intergenic 45.0%
! GCTTGCTGGATTAAGTGTGA+TGG + chr4_1:20871899-20871918 Msa0539700:CDS 45.0%
!! CACAAAGGCACTATCACACA+AGG + chr4_1:20871966-20871985 Msa0539700:CDS 45.0%
GGGCTGTCCTTTGTAGTCAA+CGG - chr4_1:20868550-20868569 None:intergenic 50.0%
ACAAAGGACAGCCCGCAATT+AGG + chr4_1:20868556-20868575 Msa0539700:CDS 50.0%
CCCGCAATTAGGTCCAAATC+TGG + chr4_1:20868567-20868586 Msa0539700:CDS 50.0%
CTGGTTATTGGAGATCCGCT+TGG + chr4_1:20868586-20868605 Msa0539700:CDS 50.0%
AGTCCGAGCTGCAAGAGATA+AGG + chr4_1:20869123-20869142 Msa0539700:intron 50.0%
GGTCCTTATCTCTTGCAGCT+CGG - chr4_1:20869129-20869148 None:intergenic 50.0%
TTTGCAGTGCTCTGGTTGAG+TGG - chr4_1:20869370-20869389 None:intergenic 50.0%
!! GCAACGAAAGCACCAAAGAG+AGG - chr4_1:20869439-20869458 None:intergenic 50.0%
CTAGTAGCCATTGGACAAGG+TGG + chr4_1:20869727-20869746 Msa0539700:CDS 50.0%
GCCCCAAAAGCTTGAACACA+AGG - chr4_1:20869760-20869779 None:intergenic 50.0%
CTCCATTGATCTCCCTTGCT+TGG - chr4_1:20871334-20871353 None:intergenic 50.0%
CCGGTGATAACTCTTGCTAG+AGG - chr4_1:20871459-20871478 None:intergenic 50.0%
GTTATCACCGGAAAACCCTC+TGG + chr4_1:20871468-20871487 Msa0539700:CDS 50.0%
! TGTGATGCCAGAGGGTTTTC+CGG - chr4_1:20871478-20871497 None:intergenic 50.0%
! GATGGCGCCGTTGACTACAA+AGG + chr4_1:20868540-20868559 Msa0539700:CDS 55.0%
TAGGTGTGGAAGTGGCAGAG+AGG + chr4_1:20869296-20869315 Msa0539700:intron 55.0%
! CAGCAGCTTTTGCAGTGCTC+TGG - chr4_1:20869378-20869397 None:intergenic 55.0%
ATCGATTGAAGCGAAGCCGG+AGG - chr4_1:20871384-20871403 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 20868318 20872254 20868318 ID=Msa0539700;Name=Msa0539700
chr4_1 mRNA 20868318 20872254 20868318 ID=Msa0539700-mRNA-1;Parent=Msa0539700;Name=Msa0539700-mRNA-1;_AED=0.26;_eAED=0.26;_QI=135|1|1|1|0.75|0.6|5|262|559
chr4_1 exon 20868318 20868335 20868318 ID=Msa0539700-mRNA-1:exon:5958;Parent=Msa0539700-mRNA-1
chr4_1 exon 20868375 20868618 20868375 ID=Msa0539700-mRNA-1:exon:5959;Parent=Msa0539700-mRNA-1
chr4_1 exon 20869299 20869516 20869299 ID=Msa0539700-mRNA-1:exon:5960;Parent=Msa0539700-mRNA-1
chr4_1 exon 20869634 20870145 20869634 ID=Msa0539700-mRNA-1:exon:5961;Parent=Msa0539700-mRNA-1
chr4_1 exon 20871170 20872254 20871170 ID=Msa0539700-mRNA-1:exon:5962;Parent=Msa0539700-mRNA-1
chr4_1 five_prime_UTR 20868318 20868335 20868318 ID=Msa0539700-mRNA-1:five_prime_utr;Parent=Msa0539700-mRNA-1
chr4_1 five_prime_UTR 20868375 20868491 20868375 ID=Msa0539700-mRNA-1:five_prime_utr;Parent=Msa0539700-mRNA-1
chr4_1 CDS 20868492 20868618 20868492 ID=Msa0539700-mRNA-1:cds;Parent=Msa0539700-mRNA-1
chr4_1 CDS 20869299 20869516 20869299 ID=Msa0539700-mRNA-1:cds;Parent=Msa0539700-mRNA-1
chr4_1 CDS 20869634 20870145 20869634 ID=Msa0539700-mRNA-1:cds;Parent=Msa0539700-mRNA-1
chr4_1 CDS 20871170 20871992 20871170 ID=Msa0539700-mRNA-1:cds;Parent=Msa0539700-mRNA-1
chr4_1 three_prime_UTR 20871993 20872254 20871993 ID=Msa0539700-mRNA-1:three_prime_utr;Parent=Msa0539700-mRNA-1
Gene Sequence

>Msa0539700

ATGGCAGAATCCAACGAAGTGTTGCTAGAAGCTCAATTAGCTCTTGTTGATGGCGCCGTTGACTACAAAGGACAGCCCGCAATTAGGTCCAAATCTGGTTATTGGAGATCCGCTTGGTTCATAATTGGTGTGGAAGTGGCAGAGAGGATATCATATTATGGAATTCAAGAGAACTTGATAACATATCTAACAGGACCACTCAACCAGAGCACTGCAAAAGCTGCTGAAAATGTGAATATTTGGGCTGGAACTGCTTCTCTACTTCCTCTCTTTGGTGCTTTCGTTGCTGATTCTTTTCTTGGACGCTACCGCACAATCATACTTGCTTCTCTCATTTATATCTTGGGACTAGGTTTGTTAACATTATCAGCTTTGCTTCCTTCCCTCACATTCTGTTCTTCTGAGTCACAAGTAATCTTATTTTTCATCTCACTCTATCTAGTAGCCATTGGACAAGGTGGACATAAACCTTGTGTTCAAGCTTTTGGGGCGGATCAATTCGACGAAAAACATCCTAAAGAGCATGAAGCTAGAAGCTCATTCTTTAATTGGTGGTATTTTACTATGATTGCAGGTTGCACATCAGTACTTACAATCTTGAACTATATACAAGATAACATTAGTTGGGTTCTTGGATTTGGAATCCCTTGCGTTGTAATGATCATTGCTTTGACTGTCCTTTTGCTTGGAACAAGGACATATAGGTTTAACATTAAGGATAATGGCAAGAGTCCCTTTCTTAGAATTGGTCGTGTATTTGTTGCGGCTGTAAGAAATTGGCGAAATAACTTATCAACGATGGATTTTGAAGAAGAATGTGATGGACTACTTCTCCGTCAAAGTTCTGAACAATTCAACTTCCTTAACAAGGCATTGCTAACACCAAAGGGATCTAAAGAAGATGAAACTTGTAGCCTTAGTGAGGTGGAAGAAGCTAAGGCTGTACTAAGGTTGGTTCCAATTTGGGCTACAACTTTGGTTTATGGTATTGTCTTTGCTCAAGTTTTTACATTCTTTACCAAGCAAGGGAGATCAATGGAGAGAACAATAGTTGCTGGATTTGACATACCTCCGGCTTCGCTTCAATCGATTAACGGGCTTGCAATTCTTTTCTTCAGCCCTATCTATGACCGAATATTTGTGCCTCTAGCAAGAGTTATCACCGGAAAACCCTCTGGCATCACAATGCTTCAAAGAATTGGCACTGGAATTTTTATATCGATATTCACGGTAGTATTTGCAGCCTTTGTTGAGATGAAAAGACTAAAAATAGCACAAGATCATGGTCTTGTTGATGATCCAAATGCAACTGTTCCAATGAGTATATGGTGGCTGGTTCCTCAATACTTTTTGTTTGGAATTTCTGAGGTTTTTACTATGGTTGGTCTTCAGGAATTTTTCTACGATCAAGTCCCAAATGAACTAAGAAGCATGGGACTTGCACTCTATTTGAGTATTGTTGGTGTTGGAAGCTTTTTAAGTGGCTTTCTCATTTCTTTGATAGAAAATTTTAGTGGCAAAGATGGACATGATAGTTGGTTTTGTGACAATATCAATAAGGCACATTTTGATTACTTTTATTGGTTGCTTGCTGGATTAAGTGTGATGGGGTTTACTTTGTTCATTTACTTTGCAAAATCTTACATTTATAATCACAAAGGCACTATCACACAAGGATAG
Protein sequence

>Msa0539700

MAESNEVLLEAQLALVDGAVDYKGQPAIRSKSGYWRSAWFIIGVEVAERISYYGIQENLITYLTGPLNQSTAKAAENVNIWAGTASLLPLFGAFVADSFLGRYRTIILASLIYILGLGLLTLSALLPSLTFCSSESQVILFFISLYLVAIGQGGHKPCVQAFGADQFDEKHPKEHEARSSFFNWWYFTMIAGCTSVLTILNYIQDNISWVLGFGIPCVVMIIALTVLLLGTRTYRFNIKDNGKSPFLRIGRVFVAAVRNWRNNLSTMDFEEECDGLLLRQSSEQFNFLNKALLTPKGSKEDETCSLSEVEEAKAVLRLVPIWATTLVYGIVFAQVFTFFTKQGRSMERTIVAGFDIPPASLQSINGLAILFFSPIYDRIFVPLARVITGKPSGITMLQRIGTGIFISIFTVVFAAFVEMKRLKIAQDHGLVDDPNATVPMSIWWLVPQYFLFGISEVFTMVGLQEFFYDQVPNELRSMGLALYLSIVGVGSFLSGFLISLIENFSGKDGHDSWFCDNINKAHFDYFYWLLAGLSVMGFTLFIYFAKSYIYNHKGTITQG*