AlfalfaGEDB | Msa0556340

M. sativa cultivar zhongmu-4 / Msa0556340

KEGG: K00924
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004713 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009937 GO:0009939 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010646 GO:0010647 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018108 GO:0018193 GO:0018209 GO:0018212 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0556340	GAU31803.1	99.213	254	2	0	194	447	111	364	0.0	520

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0556340	sp\|Q9SCS2\|CAMK5_ARATH	81.369	263	46	1	183	442	336	598	1.19e-152	449
Msa0556340	sp\|Q9SCS2\|CAMK5_ARATH	50.732	205	62	9	1	175	1	196	1.62e-41	159

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0556340	A0A2Z6MQ24	99.213	254	2	0	194	447	111	364	0.0	520

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0556340	Msa0556360	0.886057	4.761873e-72	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0556340	MtrunA17_Chr4g0045261	99.606	254	1	0	194	447	339	592	0.0	520
Msa0556340	MtrunA17_Chr4g0045261	86.170	188	10	3	1	175	1	185	3.24e-103	319
Msa0556340	MtrunA17_Chr8g0374701	83.399	253	41	1	194	446	330	581	2.66e-150	439
Msa0556340	MtrunA17_Chr8g0374701	45.408	196	66	11	1	175	1	176	6.28e-28	116
Msa0556340	MtrunA17_Chr5g0402551	81.423	253	46	1	194	446	336	587	8.65e-146	428
Msa0556340	MtrunA17_Chr5g0402551	46.809	188	81	7	1	175	1	182	1.02e-34	136
Msa0556340	MtrunA17_Chr7g0256001	72.222	252	69	1	194	444	343	594	3.79e-129	386
Msa0556340	MtrunA17_Chr7g0256001	55.882	136	48	5	50	175	56	189	2.57e-32	129
Msa0556340	MtrunA17_Chr7g0276111	62.698	252	90	3	194	444	346	594	5.41e-106	326
Msa0556340	MtrunA17_Chr7g0276111	47.887	142	57	4	51	175	51	192	1.32e-14	75.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0556340	AT3G50530.1	81.369	263	46	1	183	442	336	598	1.21e-153	449
Msa0556340	AT3G50530.1	50.732	205	62	9	1	175	1	196	1.65e-42	159
Msa0556340	AT3G50530.2	81.369	263	46	1	183	442	367	629	2.14e-153	449
Msa0556340	AT3G50530.2	50.732	205	62	9	1	175	1	196	1.29e-42	159
Msa0556340	AT1G49580.1	75.100	249	61	1	194	441	352	600	2.11e-129	387
Msa0556340	AT1G49580.1	46.602	206	71	8	1	175	1	198	5.28e-35	137
Msa0556340	AT2G41140.1	71.713	251	71	0	194	444	325	575	8.48e-129	384
Msa0556340	AT2G41140.1	58.182	110	36	3	75	175	63	171	1.10e-27	116
Msa0556340	AT3G19100.1	74.016	254	65	1	194	446	346	599	1.35e-127	382
Msa0556340	AT3G19100.1	45.226	199	78	8	1	175	1	192	2.51e-34	135

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Find 147 sgRNAs with CRISPR-Local

Find 352 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCCCTACACCGGTAAC+CGG	0.456028	4_1:+54489803	Msa0556340:CDS
AAATCTCTTGTCCAAATCCC+CGG	0.568972	4_1:-54490079	None:intergenic
AACCAAAATACTCACCCTTA+AGG	0.474068	4_1:-54493470	None:intergenic
AACTCGAAGCTCTTGATCGT+TGG	0.452769	4_1:+54493991	Msa0556340:CDS
AAGACGGAGAGGAGGGTGCC+GGG	0.390289	4_1:+54490061	Msa0556340:CDS
AATGTTAAATTAGTACACTC+AGG	0.612333	4_1:-54494743	None:intergenic
ACAACATGAACAGGGATCGA+TGG	0.588231	4_1:-54494255	None:intergenic
ACAAGGGAAGAAGGAAGCCG+CGG	0.599647	4_1:+54490029	Msa0556340:CDS
ACAGAATCAACCTTTGCTTT+AGG	0.277305	4_1:-54490234	None:intergenic
ACAGCCCTTATATGCTTCGC+CGG	0.561892	4_1:-54489994	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTGCTGCTAAATTCAAAA+AGG	+	chr4_1:54490173-54490192	Msa0556340:CDS	30.0%
TCTGCTGCTAAATTCAAAAA+GGG	+	chr4_1:54490174-54490193	Msa0556340:CDS	30.0%
ATTCAAAAAGGGTGAGTTTA+AGG	+	chr4_1:54490185-54490204	Msa0556340:CDS	30.0%
TTCAAAAAGGGTGAGTTTAA+GGG	+	chr4_1:54490186-54490205	Msa0556340:CDS	30.0%
CAGCTAGAAATAATCATAGA+AGG	-	chr4_1:54490267-54490286	None:intergenic	30.0%
TGATACTTTGATGAATCACT+TGG	+	chr4_1:54490319-54490338	Msa0556340:intron	30.0%
TTGGAATATTGTTATAGGCT+TGG	+	chr4_1:54490348-54490367	Msa0556340:intron	30.0%
TTGTTTGGAAATGTTTCTCT+AGG	+	chr4_1:54490477-54490496	Msa0556340:intron	30.0%
TATAGGAATTGAATTTGCCT+TGG	+	chr4_1:54490646-54490665	Msa0556340:intron	30.0%
ATTGAATTTGCCTTGGAATT+TGG	+	chr4_1:54490653-54490672	Msa0556340:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	54489712	54494804	54489712	ID=Msa0556340;Name=Msa0556340
chr4_1	mRNA	54489712	54494804	54489712	ID=Msa0556340-mRNA-1;Parent=Msa0556340;Name=Msa0556340-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0\|0.55\|0.5\|1\|0.55\|0.6\|10\|421\|447
chr4_1	exon	54489712	54489857	54489712	ID=Msa0556340-mRNA-1:exon:16059;Parent=Msa0556340-mRNA-1
chr4_1	exon	54489876	54490103	54489876	ID=Msa0556340-mRNA-1:exon:16060;Parent=Msa0556340-mRNA-1
chr4_1	exon	54490125	54490245	54490125	ID=Msa0556340-mRNA-1:exon:16061;Parent=Msa0556340-mRNA-1
chr4_1	exon	54492985	54493067	54492985	ID=Msa0556340-mRNA-1:exon:16062;Parent=Msa0556340-mRNA-1
chr4_1	exon	54493112	54493281	54493112	ID=Msa0556340-mRNA-1:exon:16063;Parent=Msa0556340-mRNA-1
chr4_1	exon	54493365	54493477	54493365	ID=Msa0556340-mRNA-1:exon:16064;Parent=Msa0556340-mRNA-1
chr4_1	exon	54493582	54493689	54493582	ID=Msa0556340-mRNA-1:exon:16065;Parent=Msa0556340-mRNA-1
chr4_1	exon	54493771	54493833	54493771	ID=Msa0556340-mRNA-1:exon:16066;Parent=Msa0556340-mRNA-1
chr4_1	exon	54493924	54494091	54493924	ID=Msa0556340-mRNA-1:exon:16067;Parent=Msa0556340-mRNA-1
chr4_1	exon	54494240	54494804	54494240	ID=Msa0556340-mRNA-1:exon:16068;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54489712	54489857	54489712	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54489876	54490103	54489876	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54490125	54490245	54490125	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54492985	54493067	54492985	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54493112	54493281	54493112	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54493365	54493477	54493365	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54493582	54493689	54493582	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54493771	54493833	54493771	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54493924	54494091	54493924	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	CDS	54494240	54494383	54494240	ID=Msa0556340-mRNA-1:cds;Parent=Msa0556340-mRNA-1
chr4_1	three_prime_UTR	54494384	54494804	54494384	ID=Msa0556340-mRNA-1:three_prime_utr;Parent=Msa0556340-mRNA-1

Gene Sequence

>Msa0556340

ATGGGGGGTTGCACTTCAAAGCCACAGAAACCAAACCCATATGCCCTTAGAGAAATGGAAACTGACCCAACACAAATCCCACAGACCCCACAAAGTCCCTACACCGGTAACCGGAAAGATGACGTCATCGCCGGAAAAAAGTCTCCTCCAAGTCCAGCTCGGTTTCTGAAGAAGTCTCCGTCCACTCCGTCGACTCCGGCGAGTGAAAGAAGCCGGAACTCGACGCCAAGGAGGTTTTTCCGGCGACCCTTTCCACCGCCGTCTCCGGCGAAGCATATAAGGGCTGTGTTGGCTAGGAGACAAGGGAAGAAGGAAGCCGCGGCGATACCGGAAGACGGAGAGGAGGGTGCCGGGGATTTGGACAAGAGATTTGGTAAGTTGGAGGTTGGGGAAGAAGTGGGTAGGGGGCATTTTGGGTATACTTCTGCTGCTAAATTCAAAAAGGGTGAGTTTAAGGGTCAACAAGTGGCTGTGAAAGTGATTCCTAAAGCAAAGATGAAAGGCTTAATGACATTGTTGGAAGTGCATATTATGTGGCTCCTGAAGTTCTCCATAGATCTTACAGTACAGAAGCTGATTCGCCCATTTTGGGCTCGAACGGAATCAGGCATTTTTAGGGCAGTTTTGAAAGCTGATCCAGGCTTTGACGAAGGTCCCTGGCCATCCTTATCTTCAGAAGCAAAAGATTTTGTCAAACGCCTACTAAATAAGGACCCTCGGAAGCGAATATCTGCTGCTCAGGCTCTGAGTCATCCTTGGATACGTAATTACAATGATGTAAAAGTTCCGCTTGATATTTTAATATTCAAACTCATGAAGACATATATGAGATCGTCATCCTTGCGTAAGGCTGCCTTAAGGGCTTTATCCAAGACTTTAACTGCTGATGAACTCTATTATCTGAGGGAGCAATTTGCGTTATTGGAACCAAGCAAAAACGGCAGCATATCCTTGGAGAACATTAACAAGGCTTTGAAGAAATATGCAACAGATGCCATGAAAGAGTCTCGTATCACTGATTTTCTTTCCTCGCTGAGTTCATTACAATATAGAAGGATGGATTTTGAAGAATTTTGTGCGGCTGCATTAAGTGTGCATCAACTCGAAGCTCTTGATCGTTGGGAACAACATGCTCGCTGTGCATATGAACTTTTTGAGAAAGATGGAAACAGGGCAATTGTCATTGAAGAACTAGCTTCAGAACTTGGACTTGGTCCATCGATCCCTGTTCATGTTGTTCTTCATGACTGGATACGCCACACAGATGGGAAGCTAAGCTTTCTCGGGTTTGTCAAATTATTGCATGGTGTATCTAGCCGAAGCCTTCCAAAGGTTCAGTTATGA

Protein sequence

>Msa0556340

MGGCTSKPQKPNPYALREMETDPTQIPQTPQSPYTGNRKDDVIAGKKSPPSPARFLKKSPSTPSTPASERSRNSTPRRFFRRPFPPPSPAKHIRAVLARRQGKKEAAAIPEDGEEGAGDLDKRFGKLEVGEEVGRGHFGYTSAAKFKKGEFKGQQVAVKVIPKAKMKGLMTLLEVHIMWLLKFSIDLTVQKLIRPFWARTESGIFRAVLKADPGFDEGPWPSLSSEAKDFVKRLLNKDPRKRISAAQALSHPWIRNYNDVKVPLDILIFKLMKTYMRSSSLRKAALRALSKTLTADELYYLREQFALLEPSKNGSISLENINKALKKYATDAMKESRITDFLSSLSSLQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYELFEKDGNRAIVIEELASELGLGPSIPVHVVLHDWIRHTDGKLSFLGFVKLLHGVSSRSLPKVQL*