AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0557420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0557420 MtrunA17_Chr7g0272721 77.489 231 7 1 70 255 168 398 6.41e-122 356
Msa0557420 MtrunA17_Chr7g0262361 73.160 231 17 1 69 254 167 397 1.26e-115 340
Msa0557420 MtrunA17_Chr1g0168631 60.606 231 47 1 69 255 168 398 8.19e-95 287
Msa0557420 MtrunA17_Chr1g0189971 58.051 236 50 2 69 255 167 402 9.68e-90 275
Msa0557420 MtrunA17_Chr3g0104661 57.895 228 51 2 71 254 135 361 1.61e-87 267
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0557420 AT4G28700.1 60.515 233 45 1 69 254 171 403 5.15e-93 283
Msa0557420 AT3G24290.1 53.788 264 67 2 36 255 144 396 1.48e-91 279
Msa0557420 AT4G13510.1 58.190 232 52 2 69 255 162 393 2.20e-91 278
Msa0557420 AT1G64780.1 59.227 233 49 2 69 255 175 407 1.20e-90 277
Msa0557420 AT3G24300.1 56.466 232 56 2 69 255 166 397 2.38e-86 265

Find 61 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGGTTGGCGAGCGAAAA+TGG 0.242600 4_1:-55792046 Msa0557420:CDS
CAACTGTTGCTCATTGGTTT+TGG 0.250219 4_1:-55790892 Msa0557420:CDS
TTCATGGTGGTTGTGGTACT+TGG 0.252134 4_1:-55790293 Msa0557420:CDS
CAAGGTGGAGCTCTATCTTT+TGG 0.276999 4_1:-55792073 Msa0557420:intron
TAGTGGTACTTTAGTTGTTA+TGG 0.289328 4_1:-55790681 Msa0557420:CDS
GTACCACTATGTCCTCTAAT+TGG 0.291911 4_1:+55790695 None:intergenic
TTCTAATTCAGAATCGGTTT+TGG 0.322575 4_1:+55793804 None:intergenic
CATTTAGTTGGTGTTGTTGC+TGG 0.324395 4_1:-55790782 Msa0557420:CDS
CTTGAAGCTGCTCAACTTCA+TGG 0.324413 4_1:-55790309 Msa0557420:CDS
TGATCAAATCTACCAATTCT+TGG 0.336025 4_1:+55790734 None:intergenic
TTGTGGATTTGTTGCTGCTT+GGG 0.357633 4_1:-55790382 Msa0557420:CDS
AGTGGTACTTTAGTTGTTAT+GGG 0.366370 4_1:-55790680 Msa0557420:CDS
AGATATGTGATTCTGAATAA+TGG 0.381441 4_1:+55793957 None:intergenic
TCATGGTGGTTGTGGTACTT+GGG 0.382463 4_1:-55790292 Msa0557420:CDS
CCATGGGCTGCAATTATTTG+TGG 0.385626 4_1:-55790399 Msa0557420:CDS
GATAGAAATGAGAATGAAAA+GGG 0.389573 4_1:+55794044 None:intergenic
AAAGAAGAGAATAGAGAGTA+AGG 0.401080 4_1:+55793982 None:intergenic
GGTGTTCTGTTGTGGATCCA+TGG 0.413692 4_1:-55790416 Msa0557420:CDS
AAATTACCTACGAAACTGTT+AGG 0.415788 4_1:+55792015 None:intergenic
TTTGTGGATTTGTTGCTGCT+TGG 0.421882 4_1:-55790383 Msa0557420:CDS
TTTATTGAAGGACCAAGAAT+TGG 0.431351 4_1:-55790746 Msa0557420:CDS
GGTAAAAGTGTTCCAATTAG+AGG 0.432180 4_1:-55790707 Msa0557420:CDS
ACTTTGGTTTGGTTGGTCCC+TGG 0.432231 4_1:-55790648 Msa0557420:intron
TTTGCAGAGAGAGAAAATAA+GGG 0.434389 4_1:+55793929 None:intergenic
TTTATCCAACTGTTGCTCAT+TGG 0.435136 4_1:-55790898 Msa0557420:CDS
TTTGGTAAACGTATGCAAAC+AGG 0.448699 4_1:-55790501 Msa0557420:CDS
ATCGATTTCTAATTCAGAAT+CGG 0.457650 4_1:+55793798 None:intergenic
AACGTATGCAAACAGGTCAT+TGG 0.462679 4_1:-55790494 Msa0557420:CDS
GCTCAACTTCATGGTGGTTG+TGG 0.465024 4_1:-55790300 Msa0557420:CDS
AGATTCAAAATTCAAATTGA+AGG 0.467134 4_1:+55794018 None:intergenic
GGATAGAAATGAGAATGAAA+AGG 0.481389 4_1:+55794043 None:intergenic
AACTGCAGGGTGTTCTGTTG+TGG 0.482968 4_1:-55790424 Msa0557420:CDS
GTATGTAATGGTTTACTTGG+TGG 0.490400 4_1:-55790459 Msa0557420:CDS
GAAGGTTGGCGAGCGAAAAT+GGG 0.501463 4_1:-55792045 Msa0557420:CDS
GATGTATGTAATGGTTTACT+TGG 0.503072 4_1:-55790462 Msa0557420:CDS
AAGGATTGCTTTGTCTGCCA+TGG 0.507020 4_1:-55793001 Msa0557420:CDS
GAAGAATAAGGGAAGAGAGA+TGG 0.512285 4_1:+55793885 None:intergenic
AAGAGAATAGAGAGTAAGGA+GGG 0.514542 4_1:+55793986 None:intergenic
ATGGTTGGGGTAGTCCAGTT+CGG 0.517957 4_1:-55790862 Msa0557420:CDS
CATGGTGGTTGTGGTACTTG+GGG 0.523447 4_1:-55790291 Msa0557420:CDS
GTGTTCTGTTGTGGATCCAT+GGG 0.527050 4_1:-55790415 Msa0557420:CDS
TCAAAATTCAAATTGAAGGA+AGG 0.530896 4_1:+55794022 None:intergenic
TGTTGCAGGAATGAGAAGAA+AGG 0.532498 4_1:-55793020 Msa0557420:intron
AGTGTTAGTGATGTATGTAA+TGG 0.532742 4_1:-55790471 Msa0557420:CDS
GAAGAGAATAGAGAGTAAGG+AGG 0.536412 4_1:+55793985 None:intergenic
TTGCAGAGAGAGAAAATAAG+GGG 0.537591 4_1:+55793930 None:intergenic
AAAGCGCACAAAGGAGGCGG+AGG 0.558648 4_1:-55793745 Msa0557420:intron
GAGAGATGGATGAAGATTTG+TGG 0.562733 4_1:+55793899 None:intergenic
CCACAAATAATTGCAGCCCA+TGG 0.565527 4_1:+55790399 None:intergenic
ATGGTGGTTGTGGTACTTGG+GGG 0.572938 4_1:-55790290 Msa0557420:CDS
ATTGGTAGATTTGATCATGA+GGG 0.582391 4_1:-55790728 Msa0557420:CDS
CAAAACCAATGAGCAACAGT+TGG 0.583861 4_1:+55790893 None:intergenic
AATTGGTAGATTTGATCATG+AGG 0.587107 4_1:-55790729 Msa0557420:CDS
CTTACTTGCTAACAACTCCA+TGG 0.600422 4_1:+55792984 None:intergenic
ATTCTGAATTAGAAATCGAT+CGG 0.601431 4_1:-55793795 Msa0557420:five_prime_UTR
CCATAGTTCAAACTATTGAT+CGG 0.601566 4_1:+55793597 None:intergenic
ATAGAAATGAGAATGAAAAG+GGG 0.606003 4_1:+55794045 None:intergenic
TGAAGTTGAGCAGCTTCAAG+AGG 0.615625 4_1:+55790312 None:intergenic
CTCAGTCCTAACAGTTTCGT+AGG 0.617776 4_1:-55792021 Msa0557420:intron
GTTCCAATTAGAGGACATAG+TGG 0.643396 4_1:-55790698 Msa0557420:CDS
GAAGCTGCTCAACTTCATGG+TGG 0.677955 4_1:-55790306 Msa0557420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATATAACAATAACAAT+TGG + chr4_1:55791202-55791221 None:intergenic 10.0%
!! AAGAACAAAAAAAATATTAA+CGG + chr4_1:55792374-55792393 None:intergenic 10.0%
!!! TAATACATATTGATAATAAT+TGG + chr4_1:55792817-55792836 None:intergenic 10.0%
!! ATATTTCTTATATTTAATCA+AGG + chr4_1:55792929-55792948 None:intergenic 10.0%
!! AGTAAAAAATTGTATTTATA+AGG + chr4_1:55793060-55793079 None:intergenic 10.0%
!! ATGAATTCAATAAAAAAAGA+AGG + chr4_1:55790639-55790658 None:intergenic 15.0%
!!! TTCTTTTTTTATTGAATTCA+TGG - chr4_1:55790638-55790657 Msa0557420:intron 15.0%
!!! TTGTTATATTTTGATTTTGA+TGG - chr4_1:55791232-55791251 Msa0557420:intron 15.0%
!!! ATAATAACTACTTTGTTATT+TGG - chr4_1:55791678-55791697 Msa0557420:intron 15.0%
!!! AATCTTTTAATACTAACATA+TGG - chr4_1:55791720-55791739 Msa0557420:intron 15.0%
!!! ATCTTTTAATACTAACATAT+GGG - chr4_1:55791721-55791740 Msa0557420:intron 15.0%
!! AAAAAGAAATGAAATATCAA+AGG + chr4_1:55792191-55792210 None:intergenic 15.0%
!! AAATTAACAACTAAAACTAT+CGG + chr4_1:55792522-55792541 None:intergenic 15.0%
!! CAAATGTAAATATTTATCTA+AGG + chr4_1:55793013-55793032 None:intergenic 15.0%
!! AGATTCAAAATTCAAATTGA+AGG + chr4_1:55790329-55790348 None:intergenic 20.0%
!! CGTAAAAATAAGCAAAAATT+AGG + chr4_1:55791064-55791083 None:intergenic 20.0%
!! TCAAAATAGAAAACCATATA+TGG + chr4_1:55791291-55791310 None:intergenic 20.0%
!!! TCTTAATTTTTAGTGATTCT+TGG - chr4_1:55791431-55791450 Msa0557420:intron 20.0%
!! GAAAATAACACAAGTAAAAA+AGG - chr4_1:55791559-55791578 Msa0557420:intron 20.0%
!! GAAAATAACACAAGTAAAAA+AGG - chr4_1:55791559-55791578 Msa0557420:intron 20.0%
!!! CTTTTGTTAAATATGTCATT+TGG - chr4_1:55791787-55791806 Msa0557420:intron 20.0%
!!! AGTTATGATTTTAACTAACT+AGG - chr4_1:55791985-55792004 Msa0557420:intron 20.0%
!!! TATCATTTGAAACAAAAACA+GGG + chr4_1:55792064-55792083 None:intergenic 20.0%
!!! AAGTGATATAAATTTGTTAG+AGG + chr4_1:55792245-55792264 None:intergenic 20.0%
!! TTTATATCACTTTCAATTCA+AGG - chr4_1:55792253-55792272 Msa0557420:intron 20.0%
!! CAATACTTCAATCAAATATA+TGG - chr4_1:55792617-55792636 Msa0557420:intron 20.0%
!! AAAAGATAAAAATTGAAGCA+TGG + chr4_1:55792676-55792695 None:intergenic 20.0%
!! TTTAATCAAGGCAAAAAAAT+AGG + chr4_1:55792917-55792936 None:intergenic 20.0%
! ATAGAAATGAGAATGAAAAG+GGG + chr4_1:55790302-55790321 None:intergenic 25.0%
! GATAGAAATGAGAATGAAAA+GGG + chr4_1:55790303-55790322 None:intergenic 25.0%
! TCAAAATTCAAATTGAAGGA+AGG + chr4_1:55790325-55790344 None:intergenic 25.0%
! AGATATGTGATTCTGAATAA+TGG + chr4_1:55790390-55790409 None:intergenic 25.0%
! AAGAAGAAGAAGAAGAATAA+GGG + chr4_1:55790473-55790492 None:intergenic 25.0%
!! ATCGATTTCTAATTCAGAAT+CGG + chr4_1:55790549-55790568 None:intergenic 25.0%
! ATTCTGAATTAGAAATCGAT+CGG - chr4_1:55790549-55790568 Msa0557420:intron 25.0%
! ATCACAAAAAAAAAACCCAA+AGG + chr4_1:55790819-55790838 None:intergenic 25.0%
!! TGAGTACAATTTTTATCTTC+TGG - chr4_1:55790933-55790952 Msa0557420:intron 25.0%
!!! TTCTGGAACAAATTATTCAT+AGG - chr4_1:55790950-55790969 Msa0557420:intron 25.0%
! CAATAATCTAACATAATGTC+AGG + chr4_1:55790980-55790999 None:intergenic 25.0%
!!! TGAAACATTTTTTTTTCCAC+AGG - chr4_1:55791004-55791023 Msa0557420:intron 25.0%
! GTATTTACTTTGAGCTTAAT+TGG - chr4_1:55791153-55791172 Msa0557420:intron 25.0%
!! ATACTCATGTTTTCCATATA+TGG - chr4_1:55791275-55791294 Msa0557420:intron 25.0%
! CACTAAAAATTAAGAACCAA+AGG + chr4_1:55791426-55791445 None:intergenic 25.0%
! AAGTAAAAAAGGTAAAGACA+TGG - chr4_1:55791611-55791630 Msa0557420:intron 25.0%
! GTTCTATTGAATGTGTAATT+TGG - chr4_1:55791747-55791766 Msa0557420:intron 25.0%
!! AAAAGTACATATTCAGCAAA+AGG + chr4_1:55791830-55791849 None:intergenic 25.0%
!! CTATCATTTGAAACAAAAAC+AGG + chr4_1:55792065-55792084 None:intergenic 25.0%
! CTTGTAATTAAATCATTTGC+AGG + chr4_1:55792105-55792124 None:intergenic 25.0%
! CTGCAAATGATTTAATTACA+AGG - chr4_1:55792103-55792122 Msa0557420:intron 25.0%
! TTTAATTACAAGGTTCTCAA+AGG - chr4_1:55792113-55792132 Msa0557420:intron 25.0%
! AAGGAATTAAAGTGATAGAT+TGG + chr4_1:55792172-55792191 None:intergenic 25.0%
!!! TTTGTTCTTTGTTGATTTCT+TGG - chr4_1:55792385-55792404 Msa0557420:intron 25.0%
!!! GATTGATTTGTTAGTTTGAT+TGG - chr4_1:55792480-55792499 Msa0557420:intron 25.0%
! TAAAACTATCGGAAAAACAT+GGG + chr4_1:55792511-55792530 None:intergenic 25.0%
! ACTAAAAAATTCACTGGAAA+GGG + chr4_1:55792701-55792720 None:intergenic 25.0%
! CATAAAATGACTACTATTTG+TGG + chr4_1:55792859-55792878 None:intergenic 25.0%
! AAAAGACTAATGTAAAAAGC+GGG + chr4_1:55793156-55793175 None:intergenic 25.0%
! CAAAAGACTAATGTAAAAAG+CGG + chr4_1:55793157-55793176 None:intergenic 25.0%
!! GTAAGAAATATTTTACGACA+TGG - chr4_1:55793272-55793291 Msa0557420:intron 25.0%
!!! ATTTATTCGTCGTTTTTAAC+AGG - chr4_1:55793418-55793437 Msa0557420:intron 25.0%
!!! TTATGGGAACTTTTTTACTT+TGG - chr4_1:55793680-55793699 Msa0557420:intron 25.0%
! CAATATTAAGAAAAGAACCA+GGG + chr4_1:55793716-55793735 None:intergenic 25.0%
! ACAATATTAAGAAAAGAACC+AGG + chr4_1:55793717-55793736 None:intergenic 25.0%
GGATAGAAATGAGAATGAAA+AGG + chr4_1:55790304-55790323 None:intergenic 30.0%
AAAGAAGAGAATAGAGAGTA+AGG + chr4_1:55790365-55790384 None:intergenic 30.0%
TTTGCAGAGAGAGAAAATAA+GGG + chr4_1:55790418-55790437 None:intergenic 30.0%
! TTTTGCAGAGAGAGAAAATA+AGG + chr4_1:55790419-55790438 None:intergenic 30.0%
GAAGAAGAAGAAGAAGAATA+AGG + chr4_1:55790474-55790493 None:intergenic 30.0%
!! TTCTAATTCAGAATCGGTTT+TGG + chr4_1:55790543-55790562 None:intergenic 30.0%
!! GGTTTGTTGTTGTTATAGTT+CGG - chr4_1:55790713-55790732 Msa0557420:CDS 30.0%
CCATAGTTCAAACTATTGAT+CGG + chr4_1:55790750-55790769 None:intergenic 30.0%
CTTCATGCAATTGGATTATA+AGG - chr4_1:55790770-55790789 Msa0557420:CDS 30.0%
AAAAACCCAAAGGAAAATTG+AGG + chr4_1:55790809-55790828 None:intergenic 30.0%
TTGTACTCAAAACACAACAA+TGG + chr4_1:55790922-55790941 None:intergenic 30.0%
! TTTGATTTATGCTTTCGTTG+AGG - chr4_1:55791027-55791046 Msa0557420:intron 30.0%
ACTTTATGTGATTATGTTGC+AGG - chr4_1:55791310-55791329 Msa0557420:intron 30.0%
! TTGTTAGCAAGTAAGTTTTG+TGG - chr4_1:55791368-55791387 Msa0557420:intron 30.0%
! CTAATTTTTATGCACTCCTT+TGG - chr4_1:55791407-55791426 Msa0557420:intron 30.0%
TAAATGTGTTTCGATGATTC+AGG - chr4_1:55791537-55791556 Msa0557420:intron 30.0%
CAAAGTAGTTATTATACCCA+TGG + chr4_1:55791673-55791692 None:intergenic 30.0%
! TCACTTGCATGAAATCTTTT+AGG - chr4_1:55791867-55791886 Msa0557420:intron 30.0%
ACATATTATCTTAGCTAGCT+AGG - chr4_1:55791930-55791949 Msa0557420:intron 30.0%
! AATTTAAAGTTTCCACCTCA+AGG + chr4_1:55791963-55791982 None:intergenic 30.0%
! AATTAGCTTCATTGTAGCAT+TGG - chr4_1:55792009-55792028 Msa0557420:intron 30.0%
ATGATAGAATTTGATGTACG+AGG - chr4_1:55792078-55792097 Msa0557420:intron 30.0%
ATATCACTTTCAATTCAAGG+TGG - chr4_1:55792256-55792275 Msa0557420:intron 30.0%
! CTCTATCTTTTGGATATTGA+AGG - chr4_1:55792281-55792300 Msa0557420:intron 30.0%
! ATCTTTTGGATATTGAAGGT+TGG - chr4_1:55792285-55792304 Msa0557420:intron 30.0%
AAATTACCTACGAAACTGTT+AGG + chr4_1:55792332-55792351 None:intergenic 30.0%
CAATCACAAATATTGTGTTC+AGG + chr4_1:55792465-55792484 None:intergenic 30.0%
AAAACTATCGGAAAAACATG+GGG + chr4_1:55792510-55792529 None:intergenic 30.0%
CTAAAACTATCGGAAAAACA+TGG + chr4_1:55792512-55792531 None:intergenic 30.0%
CACTAAAAAATTCACTGGAA+AGG + chr4_1:55792702-55792721 None:intergenic 30.0%
GAAACACTCTACACATAAAT+AGG + chr4_1:55792893-55792912 None:intergenic 30.0%
TTAACCATTTGCATATCATG+TGG - chr4_1:55792964-55792983 Msa0557420:intron 30.0%
AAAGACTAATGTAAAAAGCG+GGG + chr4_1:55793155-55793174 None:intergenic 30.0%
! AAGAGTACATATTCAGCAAA+AGG + chr4_1:55793213-55793232 None:intergenic 30.0%
! TCACTTGCTTGAAATCTTTT+AGG - chr4_1:55793250-55793269 Msa0557420:intron 30.0%
! TAGCTTTCTGAAAAGAAATG+TGG + chr4_1:55793374-55793393 None:intergenic 30.0%
!! GAAAATCTTTTGTTTGGTTC+TGG - chr4_1:55793508-55793527 Msa0557420:intron 30.0%
!!! TGTGGTTTTGTTCATTTAGT+TGG - chr4_1:55793550-55793569 Msa0557420:intron 30.0%
TTTATTGAAGGACCAAGAAT+TGG - chr4_1:55793598-55793617 Msa0557420:intron 30.0%
TGATCAAATCTACCAATTCT+TGG + chr4_1:55793613-55793632 None:intergenic 30.0%
AATTGGTAGATTTGATCATG+AGG - chr4_1:55793615-55793634 Msa0557420:CDS 30.0%
ATTGGTAGATTTGATCATGA+GGG - chr4_1:55793616-55793635 Msa0557420:CDS 30.0%
TAGTGGTACTTTAGTTGTTA+TGG - chr4_1:55793663-55793682 Msa0557420:intron 30.0%
AGTGGTACTTTAGTTGTTAT+GGG - chr4_1:55793664-55793683 Msa0557420:intron 30.0%
!!! GGAACTTTTTTACTTTGGTT+TGG - chr4_1:55793685-55793704 Msa0557420:intron 30.0%
!!! CTTTTTTACTTTGGTTTGGT+TGG - chr4_1:55793689-55793708 Msa0557420:intron 30.0%
AGTGTTAGTGATGTATGTAA+TGG - chr4_1:55793873-55793892 Msa0557420:five_prime_UTR 30.0%
GATGTATGTAATGGTTTACT+TGG - chr4_1:55793882-55793901 Msa0557420:five_prime_UTR 30.0%
AAGAGAATAGAGAGTAAGGA+GGG + chr4_1:55790361-55790380 None:intergenic 35.0%
TTGCAGAGAGAGAAAATAAG+GGG + chr4_1:55790417-55790436 None:intergenic 35.0%
! TTTACGAAAAAAGCGCACAA+AGG - chr4_1:55790590-55790609 Msa0557420:intron 35.0%
CTATAACAACAACAAACCAG+AGG + chr4_1:55790711-55790730 None:intergenic 35.0%
CCGATCAATAGTTTGAACTA+TGG - chr4_1:55790747-55790766 Msa0557420:CDS 35.0%
! TACACTCCTCAATTTTCCTT+TGG - chr4_1:55790800-55790819 Msa0557420:CDS 35.0%
! ACACTCCTCAATTTTCCTTT+GGG - chr4_1:55790801-55790820 Msa0557420:CDS 35.0%
! GAAAGCATAAATCAAACCTG+TGG + chr4_1:55791023-55791042 None:intergenic 35.0%
CACTAAAAGATCTACTGGAA+AGG + chr4_1:55791087-55791106 None:intergenic 35.0%
!!! AAGGTGTGTTTTGATGATTC+AGG - chr4_1:55791578-55791597 Msa0557420:intron 35.0%
AAAGGTAAAGACATGGTAGT+TGG - chr4_1:55791618-55791637 Msa0557420:intron 35.0%
TAGTTGGAAACAGAAGTAAG+AGG - chr4_1:55791634-55791653 Msa0557420:intron 35.0%
AGTTGGAAACAGAAGTAAGA+GGG - chr4_1:55791635-55791654 Msa0557420:intron 35.0%
! CATTTGAAACAAAAACAGGG+AGG + chr4_1:55792061-55792080 None:intergenic 35.0%
TCTATTGAAACCCAGAATAG+TGG - chr4_1:55792214-55792233 Msa0557420:intron 35.0%
!! CTGGATTTGGTGAACAAAAA+GGG + chr4_1:55792420-55792439 None:intergenic 35.0%
!! TCTGGATTTGGTGAACAAAA+AGG + chr4_1:55792421-55792440 None:intergenic 35.0%
CTGTGCACTAAAAAATTCAC+TGG + chr4_1:55792707-55792726 None:intergenic 35.0%
TTTGCCACATGATATGCAAA+TGG + chr4_1:55792971-55792990 None:intergenic 35.0%
TTTATCCAACTGTTGCTCAT+TGG - chr4_1:55793446-55793465 Msa0557420:intron 35.0%
! AAAAGATTTTCAGACCGAAC+TGG + chr4_1:55793499-55793518 None:intergenic 35.0%
! CGGTCTGAAAATCTTTTGTT+TGG - chr4_1:55793502-55793521 Msa0557420:intron 35.0%
!!! TCTGGTGTTATTGATTTTGC+TGG - chr4_1:55793526-55793545 Msa0557420:intron 35.0%
!!! GTTATTGATTTTGCTGGTTG+TGG - chr4_1:55793532-55793551 Msa0557420:intron 35.0%
!!! TTTTGGGGTGCTTTTATTGA+AGG - chr4_1:55793586-55793605 Msa0557420:intron 35.0%
!! GGTAAAAGTGTTCCAATTAG+AGG - chr4_1:55793637-55793656 Msa0557420:intron 35.0%
GTGAAAGCTTATAGTGAAAG+TGG - chr4_1:55793735-55793754 Msa0557420:intron 35.0%
AGTGAAAGTGGTCATTACTA+TGG - chr4_1:55793747-55793766 Msa0557420:CDS 35.0%
TTTGGTAAACGTATGCAAAC+AGG - chr4_1:55793843-55793862 Msa0557420:five_prime_UTR 35.0%
GTATGTAATGGTTTACTTGG+TGG - chr4_1:55793885-55793904 Msa0557420:five_prime_UTR 35.0%
GAAGAGAATAGAGAGTAAGG+AGG + chr4_1:55790362-55790381 None:intergenic 40.0%
GAGAGATGGATGAAGATTTG+TGG + chr4_1:55790448-55790467 None:intergenic 40.0%
GAAGAATAAGGGAAGAGAGA+TGG + chr4_1:55790462-55790481 None:intergenic 40.0%
GAACTATGGCTTCATGCAAT+TGG - chr4_1:55790761-55790780 Msa0557420:CDS 40.0%
CTGTGCACTAAAAGATCTAC+TGG + chr4_1:55791092-55791111 None:intergenic 40.0%
TGTTGCAGGAATGAGAAGAA+AGG - chr4_1:55791324-55791343 Msa0557420:intron 40.0%
! CTTACTTGCTAACAACTCCA+TGG + chr4_1:55791363-55791382 None:intergenic 40.0%
TATCTTAGCTAGCTAGGAGT+AGG - chr4_1:55791936-55791955 Msa0557420:intron 40.0%
! TTGCATTTCTGCCGTGTTTT+TGG + chr4_1:55793110-55793129 None:intergenic 40.0%
CAAAACCAATGAGCAACAGT+TGG + chr4_1:55793454-55793473 None:intergenic 40.0%
!!! CAACTGTTGCTCATTGGTTT+TGG - chr4_1:55793452-55793471 Msa0557420:intron 40.0%
!!! CATTGGTTTTGGTCTGCTAA+TGG - chr4_1:55793463-55793482 Msa0557420:intron 40.0%
!! GTTTTGGTCTGCTAATGGTT+GGG - chr4_1:55793468-55793487 Msa0557420:intron 40.0%
! TTTTGGTCTGCTAATGGTTG+GGG - chr4_1:55793469-55793488 Msa0557420:intron 40.0%
!! CATTTAGTTGGTGTTGTTGC+TGG - chr4_1:55793562-55793581 Msa0557420:intron 40.0%
!!! TTGGTGTTGTTGCTGGTTTT+TGG - chr4_1:55793569-55793588 Msa0557420:intron 40.0%
!!! TGGTGTTGTTGCTGGTTTTT+GGG - chr4_1:55793570-55793589 Msa0557420:intron 40.0%
GTTCCAATTAGAGGACATAG+TGG - chr4_1:55793646-55793665 Msa0557420:intron 40.0%
GTACCACTATGTCCTCTAAT+TGG + chr4_1:55793652-55793671 None:intergenic 40.0%
GTGGTCATTACTATGGTCAA+TGG - chr4_1:55793754-55793773 Msa0557420:CDS 40.0%
! TATGGTCAATGGAGTGCTAT+TGG - chr4_1:55793765-55793784 Msa0557420:CDS 40.0%
! ATGGTCAATGGAGTGCTATT+GGG - chr4_1:55793766-55793785 Msa0557420:CDS 40.0%
AACGTATGCAAACAGGTCAT+TGG - chr4_1:55793850-55793869 Msa0557420:five_prime_UTR 40.0%
! GTTTTGCTGCTATAACTGCA+GGG - chr4_1:55793907-55793926 Msa0557420:five_prime_UTR 40.0%
!! TTTGTGGATTTGTTGCTGCT+TGG - chr4_1:55793961-55793980 Msa0557420:five_prime_UTR 40.0%
!! TTGTGGATTTGTTGCTGCTT+GGG - chr4_1:55793962-55793981 Msa0557420:five_prime_UTR 40.0%
!! GTTGCTGCTTGGGTTTTAAT+TGG - chr4_1:55793972-55793991 Msa0557420:five_prime_UTR 40.0%
! ACGAAAAAAGCGCACAAAGG+AGG - chr4_1:55790593-55790612 Msa0557420:intron 45.0%
! AAAAAAGCGCACAAAGGAGG+CGG - chr4_1:55790596-55790615 Msa0557420:intron 45.0%
ACAACAAACCAGAGGAAGAG+AGG + chr4_1:55790703-55790722 None:intergenic 45.0%
AAGGATTGCTTTGTCTGCCA+TGG - chr4_1:55791343-55791362 Msa0557420:intron 45.0%
!! ACAGAAGTAAGAGGGTGCTA+TGG - chr4_1:55791643-55791662 Msa0557420:intron 45.0%
! AGGGTGCTATGGATATCCAT+GGG - chr4_1:55791654-55791673 Msa0557420:intron 45.0%
AGAGGCTATGCCACTATTCT+GGG + chr4_1:55792227-55792246 None:intergenic 45.0%
TAGAGGCTATGCCACTATTC+TGG + chr4_1:55792228-55792247 None:intergenic 45.0%
! CAAGGTGGAGCTCTATCTTT+TGG - chr4_1:55792271-55792290 Msa0557420:intron 45.0%
CTCAGTCCTAACAGTTTCGT+AGG - chr4_1:55792323-55792342 Msa0557420:intron 45.0%
!! CAGACTTGCAGTTCTGGATT+TGG + chr4_1:55792433-55792452 None:intergenic 45.0%
!!! CATTTCTGCCGTGTTTTTGG+AGG + chr4_1:55793107-55793126 None:intergenic 45.0%
!! GGTTTTGGTCTGCTAATGGT+TGG - chr4_1:55793467-55793486 Msa0557420:intron 45.0%
!!! GGTGTTGTTGCTGGTTTTTG+GGG - chr4_1:55793571-55793590 Msa0557420:intron 45.0%
GACAGCTGTCACAACTACAT+TGG - chr4_1:55793791-55793810 Msa0557420:exon 45.0%
GCAGCTCTCACTACTTAGTT+TGG - chr4_1:55793825-55793844 Msa0557420:five_prime_UTR 45.0%
! GGTTTTGCTGCTATAACTGC+AGG - chr4_1:55793906-55793925 Msa0557420:five_prime_UTR 45.0%
GTGTTCTGTTGTGGATCCAT+GGG - chr4_1:55793929-55793948 Msa0557420:five_prime_UTR 45.0%
CCACAAATAATTGCAGCCCA+TGG + chr4_1:55793948-55793967 None:intergenic 45.0%
! CCATGGGCTGCAATTATTTG+TGG - chr4_1:55793945-55793964 Msa0557420:five_prime_UTR 45.0%
TGAAGTTGAGCAGCTTCAAG+AGG + chr4_1:55794035-55794054 None:intergenic 45.0%
CTTGAAGCTGCTCAACTTCA+TGG - chr4_1:55794035-55794054 Msa0557420:five_prime_UTR 45.0%
TTCATGGTGGTTGTGGTACT+TGG - chr4_1:55794051-55794070 Msa0557420:five_prime_UTR 45.0%
TCATGGTGGTTGTGGTACTT+GGG - chr4_1:55794052-55794071 Msa0557420:five_prime_UTR 45.0%
TCTCTCTACCTCTCTTCCTC+TGG - chr4_1:55790692-55790711 Msa0557420:CDS 50.0%
!! GAGGGTGCTATGGATATCCA+TGG - chr4_1:55791653-55791672 Msa0557420:intron 50.0%
TAGCTAGGAGTAGGACCTTG+AGG - chr4_1:55791945-55791964 Msa0557420:intron 50.0%
TGAAGGTTGGCGAGCGAAAA+TGG - chr4_1:55792298-55792317 Msa0557420:intron 50.0%
GAAGGTTGGCGAGCGAAAAT+GGG - chr4_1:55792299-55792318 Msa0557420:intron 50.0%
GAGTCCCTCCTCCAAAAACA+CGG - chr4_1:55793096-55793115 Msa0557420:intron 50.0%
!!! TCTGCCGTGTTTTTGGAGGA+GGG + chr4_1:55793103-55793122 None:intergenic 50.0%
!!! TTCTGCCGTGTTTTTGGAGG+AGG + chr4_1:55793104-55793123 None:intergenic 50.0%
GCGGGGCATATATGCAGATA+TGG + chr4_1:55793138-55793157 None:intergenic 50.0%
ATGGTTGGGGTAGTCCAGTT+CGG - chr4_1:55793482-55793501 Msa0557420:intron 50.0%
!! ACTTTGGTTTGGTTGGTCCC+TGG - chr4_1:55793696-55793715 Msa0557420:intron 50.0%
! GTCAATGGAGTGCTATTGGG+AGG - chr4_1:55793769-55793788 Msa0557420:CDS 50.0%
GCTGTCACAACTACATTGGC+TGG - chr4_1:55793795-55793814 Msa0557420:five_prime_UTR 50.0%
! AACTGCAGGGTGTTCTGTTG+TGG - chr4_1:55793920-55793939 Msa0557420:five_prime_UTR 50.0%
GGTGTTCTGTTGTGGATCCA+TGG - chr4_1:55793928-55793947 Msa0557420:five_prime_UTR 50.0%
GAAGCTGCTCAACTTCATGG+TGG - chr4_1:55794038-55794057 Msa0557420:five_prime_UTR 50.0%
GCTCAACTTCATGGTGGTTG+TGG - chr4_1:55794044-55794063 Msa0557420:five_prime_UTR 50.0%
CATGGTGGTTGTGGTACTTG+GGG - chr4_1:55794053-55794072 Msa0557420:five_prime_UTR 50.0%
ATGGTGGTTGTGGTACTTGG+GGG - chr4_1:55794054-55794073 Msa0557420:five_prime_UTR 50.0%
CTAGGAGTAGGACCTTGAGG+TGG - chr4_1:55791948-55791967 Msa0557420:intron 55.0%
ACTGCGCAGACTTGCAGTTC+TGG + chr4_1:55792439-55792458 None:intergenic 55.0%
AAAGCGCACAAAGGAGGCGG+AGG - chr4_1:55790599-55790618 Msa0557420:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 55790245 55794121 55790245 ID=Msa0557420;Name=Msa0557420
chr4_1 mRNA 55790245 55794121 55790245 ID=Msa0557420-mRNA-1;Parent=Msa0557420;Name=Msa0557420-mRNA-1;_AED=0.22;_eAED=0.22;_QI=329|0.33|0.42|0.85|0.66|0.57|7|0|255
chr4_1 exon 55790245 55790523 55790245 ID=Msa0557420-mRNA-1:exon:16732;Parent=Msa0557420-mRNA-1
chr4_1 exon 55790649 55790926 55790649 ID=Msa0557420-mRNA-1:exon:16731;Parent=Msa0557420-mRNA-1
chr4_1 exon 55792022 55792091 55792022 ID=Msa0557420-mRNA-1:exon:16730;Parent=Msa0557420-mRNA-1
chr4_1 exon 55792786 55792807 55792786 ID=Msa0557420-mRNA-1:exon:16729;Parent=Msa0557420-mRNA-1
chr4_1 exon 55792989 55793034 55792989 ID=Msa0557420-mRNA-1:exon:16728;Parent=Msa0557420-mRNA-1
chr4_1 exon 55793610 55793635 55793610 ID=Msa0557420-mRNA-1:exon:16727;Parent=Msa0557420-mRNA-1
chr4_1 exon 55793746 55794121 55793746 ID=Msa0557420-mRNA-1:exon:16726;Parent=Msa0557420-mRNA-1
chr4_1 five_prime_UTR 55793793 55794121 55793793 ID=Msa0557420-mRNA-1:five_prime_utr;Parent=Msa0557420-mRNA-1
chr4_1 CDS 55793746 55793792 55793746 ID=Msa0557420-mRNA-1:cds;Parent=Msa0557420-mRNA-1
chr4_1 CDS 55793610 55793635 55793610 ID=Msa0557420-mRNA-1:cds;Parent=Msa0557420-mRNA-1
chr4_1 CDS 55792989 55793034 55792989 ID=Msa0557420-mRNA-1:cds;Parent=Msa0557420-mRNA-1
chr4_1 CDS 55792786 55792807 55792786 ID=Msa0557420-mRNA-1:cds;Parent=Msa0557420-mRNA-1
chr4_1 CDS 55792022 55792091 55792022 ID=Msa0557420-mRNA-1:cds;Parent=Msa0557420-mRNA-1
chr4_1 CDS 55790649 55790926 55790649 ID=Msa0557420-mRNA-1:cds;Parent=Msa0557420-mRNA-1
chr4_1 CDS 55790245 55790523 55790245 ID=Msa0557420-mRNA-1:cds;Parent=Msa0557420-mRNA-1
Gene Sequence

>Msa0557420

ATGAAACAAAGTCGATTTTACGAAAAAAGCGCACAAAGGAGGCGGAGTTCGGAAACGACGACTCCGATCAATAGAATGAGAAGAAAGGATTGCTTTGTCTGCCATGGAGTTGTTAGCAAGAAAATAACACAAGTAAAAAAGGTGGAGCTCTATCTTTTGGATATTGAAGGTTGGCGAGCGAAAATGGGACTCAGTCCTAACAGTTTCGTAGGTTTAGTTTATCCAACTGTTGCTCATTGGTTTTGGTCTGCTAATGGTTGGGGTAGTCCAGTTCGGTCTGAAAATCTTTTGTTTGGTTCTGGTGTTATTGATTTTGCTGGTTGTGGTTTTGTTCATTTAGTTGGTGTTGTTGCTGGTTTTTGGGGTGCTTTTATTGAAGGACCAAGAATTGGTAGATTTGATCATGAGGGTAAAAGTGTTCCAATTAGAGGACATAGTGGTACTTTAGTTGTTATGGGAACTTTTTTACTTTGGTTTGGTTGGTCCCTGTTTGGTAAACGTATGCAAACAGGTCATTGGAGTGTTAGTGATGTATGTAATGGTTTACTTGGTGGTTTTGCTGCTATAACTGCAGGGTGTTCTGTTGTGGATCCATGGGCTGCAATTATTTGTGGATTTGTTGCTGCTTGGGTTTTAATTGGATGCAATTTGTTAGCTGAGAAATTTCACTATGATGATCCTCTTGAAGCTGCTCAACTTCATGGTGGTTGTGGTACTTGGGGGATTATTTTCACTGCTTTGTTTGCTAAGAAACAATATGTTAACTAA
Protein sequence

>Msa0557420

MKQSRFYEKSAQRRRSSETTTPINRMRRKDCFVCHGVVSKKITQVKKVELYLLDIEGWRAKMGLSPNSFVGLVYPTVAHWFWSANGWGSPVRSENLLFGSGVIDFAGCGFVHLVGVVAGFWGAFIEGPRIGRFDHEGKSVPIRGHSGTLVVMGTFLLWFGWSLFGKRMQTGHWSVSDVCNGLLGGFAAITAGCSVVDPWAAIICGFVAAWVLIGCNLLAEKFHYDDPLEAAQLHGGCGTWGIIFTALFAKKQYVN*