Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0567760 | XP_013447005.1 | 87.654 | 81 | 10 | 0 | 2 | 82 | 40 | 120 | 1.54e-39 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0567760 | A0A072TUC9 | 87.654 | 81 | 10 | 0 | 2 | 82 | 40 | 120 | 7.36e-40 | 142 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0567720 | Msa0567760 | 0.802612 | 5.164352e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0567760 | MtrunA17_Chr8g0386421 | 87.654 | 81 | 10 | 0 | 2 | 82 | 40 | 120 | 1.42e-43 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0567760 | AT2G32500.1 | 63.158 | 76 | 26 | 2 | 8 | 82 | 25 | 99 | 8.23e-24 | 90.1 |
| Msa0567760 | AT2G32500.2 | 63.158 | 76 | 26 | 2 | 8 | 82 | 25 | 99 | 5.61e-23 | 89.0 |
Find 19 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATACCACCCATTTGATAT+TGG | 0.215952 | 4_1:-69110962 | None:intergenic |
| ACTCTCATCTGATTCTTCTT+TGG | 0.275343 | 4_1:-69110684 | None:intergenic |
| GGTATTATTGCTATTTCATT+AGG | 0.307379 | 4_1:+69110978 | Msa0567760:CDS |
| AATACCACCCATTTGATATT+GGG | 0.311535 | 4_1:-69110961 | None:intergenic |
| TTTCAGCTTGCCAGTGGAAT+GGG | 0.373534 | 4_1:+69110649 | Msa0567760:intron |
| TCATCTGATTCTTCTTTGGT+TGG | 0.376105 | 4_1:-69110680 | None:intergenic |
| TTTCCTCTGATAAGCGAGAT+TGG | 0.384256 | 4_1:-69110733 | None:intergenic |
| GATAAGCGAGATTGGAGCTT+TGG | 0.414590 | 4_1:-69110725 | None:intergenic |
| GTACACAACCCAATATCAAA+TGG | 0.421767 | 4_1:+69110953 | Msa0567760:CDS |
| ATCTCGCTTATCAGAGGAAA+AGG | 0.455399 | 4_1:+69110736 | Msa0567760:CDS |
| GGCTCTTGAACCCATTCCAC+TGG | 0.483835 | 4_1:-69110659 | None:intergenic |
| GAGAGTGTTTATAATACTCC+TGG | 0.488346 | 4_1:-69111021 | None:intergenic |
| TACACAACCCAATATCAAAT+GGG | 0.498209 | 4_1:+69110954 | Msa0567760:CDS |
| TTCATGTAGAAGAAAAGTTG+TGG | 0.555269 | 4_1:+69110860 | Msa0567760:intron |
| AAAGGAGAAATTATCTGAAG+AGG | 0.557579 | 4_1:+69110905 | Msa0567760:CDS |
| AAATTTAAGTTAGTTTAACC+AGG | 0.568870 | 4_1:+69111003 | Msa0567760:exon |
| CATTTGTCTGCTCAAAGCAA+AGG | 0.608614 | 4_1:+69110887 | Msa0567760:CDS |
| GCTCCAATCTCGCTTATCAG+AGG | 0.623806 | 4_1:+69110730 | Msa0567760:CDS |
| ACAACCCAATATCAAATGGG+TGG | 0.673588 | 4_1:+69110957 | Msa0567760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAATGTTATTACGTATT+AGG | + | chr4_1:69110777-69110796 | Msa0567760:intron | 15.0% |
| !! | AATCATAATGACATGAAAAA+TGG | - | chr4_1:69110813-69110832 | None:intergenic | 20.0% |
| !!! | AAATTTAAGTTAGTTTAACC+AGG | + | chr4_1:69111003-69111022 | Msa0567760:exon | 20.0% |
| !!! | GGTATTATTGCTATTTCATT+AGG | + | chr4_1:69110978-69110997 | Msa0567760:CDS | 25.0% |
| AAAGGTAAAGGTAAAGATAG+AGG | - | chr4_1:69110536-69110555 | None:intergenic | 30.0% | |
| AAAGGTAAAGGTAAAGGTAA+AGG | - | chr4_1:69110542-69110561 | None:intergenic | 30.0% | |
| TTCATGTAGAAGAAAAGTTG+TGG | + | chr4_1:69110860-69110879 | Msa0567760:intron | 30.0% | |
| AAAGGAGAAATTATCTGAAG+AGG | + | chr4_1:69110905-69110924 | Msa0567760:CDS | 30.0% | |
| TACACAACCCAATATCAAAT+GGG | + | chr4_1:69110954-69110973 | Msa0567760:CDS | 30.0% | |
| AATACCACCCATTTGATATT+GGG | - | chr4_1:69110964-69110983 | None:intergenic | 30.0% | |
| TAATACCACCCATTTGATAT+TGG | - | chr4_1:69110965-69110984 | None:intergenic | 30.0% | |
| !! | AAGGTAAAGATAGAGGTGTT+GGG | - | chr4_1:69110529-69110548 | None:intergenic | 35.0% |
| ! | AAAGGTAAAGATAGAGGTGT+TGG | - | chr4_1:69110530-69110549 | None:intergenic | 35.0% |
| CCTTGTAAAGGTAAAGGTAA+AGG | - | chr4_1:69110554-69110573 | None:intergenic | 35.0% | |
| CCTTTACCTTTACCTTTACA+AGG | + | chr4_1:69110551-69110570 | Msa0567760:intron | 35.0% | |
| TACCTTTACCTTTACAAGGT+CGG | + | chr4_1:69110555-69110574 | Msa0567760:intron | 35.0% | |
| TCATCTGATTCTTCTTTGGT+TGG | - | chr4_1:69110683-69110702 | None:intergenic | 35.0% | |
| ACTCTCATCTGATTCTTCTT+TGG | - | chr4_1:69110687-69110706 | None:intergenic | 35.0% | |
| GTACACAACCCAATATCAAA+TGG | + | chr4_1:69110953-69110972 | Msa0567760:CDS | 35.0% | |
| !! | GAGAGTGTTTATAATACTCC+TGG | - | chr4_1:69111024-69111043 | None:intergenic | 35.0% |
| !! | AGGTAAAGATAGAGGTGTTG+GGG | - | chr4_1:69110528-69110547 | None:intergenic | 40.0% |
| AACCGACCTTGTAAAGGTAA+AGG | - | chr4_1:69110560-69110579 | None:intergenic | 40.0% | |
| TGAAGTAACCGACCTTGTAA+AGG | - | chr4_1:69110566-69110585 | None:intergenic | 40.0% | |
| CTCAAATCAATGCATGCATG+CGG | - | chr4_1:69110605-69110624 | None:intergenic | 40.0% | |
| !!! | TTTCTTTTTCAGCTTGCCAG+TGG | + | chr4_1:69110643-69110662 | Msa0567760:intron | 40.0% |
| TTTCCTCTGATAAGCGAGAT+TGG | - | chr4_1:69110736-69110755 | None:intergenic | 40.0% | |
| ATCTCGCTTATCAGAGGAAA+AGG | + | chr4_1:69110736-69110755 | Msa0567760:CDS | 40.0% | |
| CATTTGTCTGCTCAAAGCAA+AGG | + | chr4_1:69110887-69110906 | Msa0567760:CDS | 40.0% | |
| ACAACCCAATATCAAATGGG+TGG | + | chr4_1:69110957-69110976 | Msa0567760:CDS | 40.0% | |
| CATGCATGCGGTAACAAATG+AGG | - | chr4_1:69110593-69110612 | None:intergenic | 45.0% | |
| ! | TTTTCAGCTTGCCAGTGGAA+TGG | + | chr4_1:69110648-69110667 | Msa0567760:intron | 45.0% |
| TTTCAGCTTGCCAGTGGAAT+GGG | + | chr4_1:69110649-69110668 | Msa0567760:intron | 45.0% | |
| GATAAGCGAGATTGGAGCTT+TGG | - | chr4_1:69110728-69110747 | None:intergenic | 45.0% | |
| GTGTTGGGGTGAATTGACGA+GGG | - | chr4_1:69110514-69110533 | None:intergenic | 50.0% | |
| GCTCCAATCTCGCTTATCAG+AGG | + | chr4_1:69110730-69110749 | Msa0567760:CDS | 50.0% | |
| ! | GGTGTTGGGGTGAATTGACG+AGG | - | chr4_1:69110515-69110534 | None:intergenic | 55.0% |
| GGCTCTTGAACCCATTCCAC+TGG | - | chr4_1:69110662-69110681 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 69110467 | 69111069 | 69110467 | ID=Msa0567760;Name=Msa0567760 |
| chr4_1 | mRNA | 69110467 | 69111069 | 69110467 | ID=Msa0567760-mRNA-1;Parent=Msa0567760;Name=Msa0567760-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0.5|0.33|1|0.5|0.66|3|59|82 |
| chr4_1 | exon | 69110467 | 69110470 | 69110467 | ID=Msa0567760-mRNA-1:exon:23067;Parent=Msa0567760-mRNA-1 |
| chr4_1 | exon | 69110655 | 69110757 | 69110655 | ID=Msa0567760-mRNA-1:exon:23068;Parent=Msa0567760-mRNA-1 |
| chr4_1 | exon | 69110869 | 69111069 | 69110869 | ID=Msa0567760-mRNA-1:exon:23069;Parent=Msa0567760-mRNA-1 |
| chr4_1 | CDS | 69110467 | 69110470 | 69110467 | ID=Msa0567760-mRNA-1:cds;Parent=Msa0567760-mRNA-1 |
| chr4_1 | CDS | 69110655 | 69110757 | 69110655 | ID=Msa0567760-mRNA-1:cds;Parent=Msa0567760-mRNA-1 |
| chr4_1 | CDS | 69110869 | 69111010 | 69110869 | ID=Msa0567760-mRNA-1:cds;Parent=Msa0567760-mRNA-1 |
| chr4_1 | three_prime_UTR | 69111011 | 69111069 | 69111011 | ID=Msa0567760-mRNA-1:three_prime_utr;Parent=Msa0567760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
