Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0572340 | Msa0946320 | 0.841923 | 3.539013e-58 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0572340 | MtrunA17_Chr7g0226401 | 46.377 | 69 | 34 | 2 | 1 | 67 | 42 | 109 | 4.40e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGTTGGGTAGTGGAATATT+TGG | 0.290473 | 4_1:-75252683 | Msa0572340:CDS |
| TTGTGAAGAGAGGAGGAAAA+TGG | 0.373624 | 4_1:+75252626 | None:intergenic |
| CCCAACTCTCCAGATTTCAT+TGG | 0.402631 | 4_1:+75252698 | None:intergenic |
| AAGTGAATTGAGCCAACAGC+TGG | 0.428530 | 4_1:-75252574 | Msa0572340:intron |
| TAGGTCGTCGATGACGAGAT+TGG | 0.430805 | 4_1:-75252530 | Msa0572340:CDS |
| GATGGCGATCCAATGAAATC+TGG | 0.459794 | 4_1:-75252707 | None:intergenic |
| CCAATGAAATCTGGAGAGTT+GGG | 0.467398 | 4_1:-75252698 | Msa0572340:CDS |
| AAATCTGGAGAGTTGGGTAG+TGG | 0.474905 | 4_1:-75252692 | Msa0572340:CDS |
| TCCAATGAAATCTGGAGAGT+TGG | 0.475376 | 4_1:-75252699 | None:intergenic |
| AGGTCGTCGATGACGAGATT+GGG | 0.488958 | 4_1:-75252529 | Msa0572340:CDS |
| CTCCTCATCAACCCAGCTGT+TGG | 0.517985 | 4_1:+75252562 | None:intergenic |
| GTAGATTCATGATCTACTCT+CGG | 0.540777 | 4_1:+75252653 | None:intergenic |
| TGGGTAAAGAAGTGAACGTA+AGG | 0.549144 | 4_1:-75252510 | Msa0572340:CDS |
| AACGGTGGAAGACAAAATCT+AGG | 0.551146 | 4_1:-75252605 | Msa0572340:CDS |
| TGATGAGGAGAGCGGTGCAT+AGG | 0.566477 | 4_1:-75252549 | Msa0572340:CDS |
| AGCCAACAGCTGGGTTGATG+AGG | 0.568668 | 4_1:-75252564 | Msa0572340:CDS |
| TAGATTCATGATCTACTCTC+GGG | 0.570344 | 4_1:+75252654 | None:intergenic |
| TCTTCCACCGTTGTGAAGAG+AGG | 0.571509 | 4_1:+75252616 | None:intergenic |
| TCCTCCTCTCTTCACAACGG+TGG | 0.606395 | 4_1:-75252620 | Msa0572340:CDS |
| TCCACCGTTGTGAAGAGAGG+AGG | 0.633470 | 4_1:+75252619 | None:intergenic |
| AGTGAATTGAGCCAACAGCT+GGG | 0.637281 | 4_1:-75252573 | Msa0572340:intron |
| AGCTGGGTTGATGAGGAGAG+CGG | 0.686144 | 4_1:-75252557 | Msa0572340:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAGATTCATGATCTACTCTC+GGG | + | chr4_1:75252491-75252510 | None:intergenic | 35.0% | |
| GTAGATTCATGATCTACTCT+CGG | + | chr4_1:75252492-75252511 | None:intergenic | 35.0% | |
| ! | GTTTTGTGTTGTGTGGTTTT+TGG | - | chr4_1:75252654-75252673 | Msa0572340:CDS | 35.0% |
| !! | GAGTTGGGTAGTGGAATATT+TGG | - | chr4_1:75252459-75252478 | Msa0572340:CDS | 40.0% |
| TTGTGAAGAGAGGAGGAAAA+TGG | + | chr4_1:75252519-75252538 | None:intergenic | 40.0% | |
| ! | TTTTCCTCCTCTCTTCACAA+CGG | - | chr4_1:75252519-75252538 | Msa0572340:CDS | 40.0% |
| AACGGTGGAAGACAAAATCT+AGG | - | chr4_1:75252537-75252556 | Msa0572340:CDS | 40.0% | |
| TGGGTAAAGAAGTGAACGTA+AGG | - | chr4_1:75252632-75252651 | Msa0572340:CDS | 40.0% | |
| !! | ACGTAAGGTTTTGTGTTGTG+TGG | - | chr4_1:75252647-75252666 | Msa0572340:CDS | 40.0% |
| !!! | TGTGTTGTGTGGTTTTTGGT+TGG | - | chr4_1:75252658-75252677 | Msa0572340:CDS | 40.0% |
| AAATCTGGAGAGTTGGGTAG+TGG | - | chr4_1:75252450-75252469 | Msa0572340:CDS | 45.0% | |
| AAGTGAATTGAGCCAACAGC+TGG | - | chr4_1:75252568-75252587 | Msa0572340:intron | 45.0% | |
| AGTGAATTGAGCCAACAGCT+GGG | - | chr4_1:75252569-75252588 | Msa0572340:intron | 45.0% | |
| !!! | TGGTTTTTGGTTGGCGATGA+TGG | - | chr4_1:75252667-75252686 | Msa0572340:CDS | 45.0% |
| TCTTCCACCGTTGTGAAGAG+AGG | + | chr4_1:75252529-75252548 | None:intergenic | 50.0% | |
| TAGGTCGTCGATGACGAGAT+TGG | - | chr4_1:75252612-75252631 | Msa0572340:CDS | 50.0% | |
| AGGTCGTCGATGACGAGATT+GGG | - | chr4_1:75252613-75252632 | Msa0572340:CDS | 50.0% | |
| TCCTCCTCTCTTCACAACGG+TGG | - | chr4_1:75252522-75252541 | Msa0572340:CDS | 55.0% | |
| TCCACCGTTGTGAAGAGAGG+AGG | + | chr4_1:75252526-75252545 | None:intergenic | 55.0% | |
| !! | AGCCAACAGCTGGGTTGATG+AGG | - | chr4_1:75252578-75252597 | Msa0572340:intron | 55.0% |
| CTCCTCATCAACCCAGCTGT+TGG | + | chr4_1:75252583-75252602 | None:intergenic | 55.0% | |
| !! | AGCTGGGTTGATGAGGAGAG+CGG | - | chr4_1:75252585-75252604 | Msa0572340:intron | 55.0% |
| TGATGAGGAGAGCGGTGCAT+AGG | - | chr4_1:75252593-75252612 | Msa0572340:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 75252447 | 75252717 | 75252447 | ID=Msa0572340;Name=Msa0572340 |
| chr4_1 | mRNA | 75252447 | 75252717 | 75252447 | ID=Msa0572340-mRNA-1;Parent=Msa0572340;Name=Msa0572340-mRNA-1;_AED=0.53;_eAED=0.60;_QI=0|0|0|1|0|0|2|0|86 |
| chr4_1 | exon | 75252595 | 75252717 | 75252595 | ID=Msa0572340-mRNA-1:exon:26065;Parent=Msa0572340-mRNA-1 |
| chr4_1 | exon | 75252447 | 75252584 | 75252447 | ID=Msa0572340-mRNA-1:exon:26064;Parent=Msa0572340-mRNA-1 |
| chr4_1 | CDS | 75252595 | 75252717 | 75252595 | ID=Msa0572340-mRNA-1:cds;Parent=Msa0572340-mRNA-1 |
| chr4_1 | CDS | 75252447 | 75252584 | 75252447 | ID=Msa0572340-mRNA-1:cds;Parent=Msa0572340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
