Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0583540 | AES69095.1 | 59.280 | 1611 | 522 | 31 | 1 | 1522 | 1 | 1566 | 0.0 | 1579 |
| Msa0583540 | AES69095.1 | 77.574 | 709 | 148 | 3 | 837 | 1536 | 774 | 1480 | 0.0 | 982 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0583540 | sp|Q9LRR4|R13L1_ARATH | 35.340 | 1030 | 555 | 21 | 5 | 944 | 4 | 1012 | 7.24e-173 | 550 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0398030 | Msa0583540 | 0.962113 | 1.714312e-120 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0583540 | MtrunA17_Chr3g0085651 | 64.636 | 1346 | 412 | 19 | 1 | 1291 | 1 | 1337 | 0.0 | 1567 |
| Msa0583540 | MtrunA17_Chr3g0085651 | 69.057 | 488 | 131 | 6 | 1062 | 1536 | 773 | 1253 | 0.0 | 623 |
| Msa0583540 | MtrunA17_Chr3g0085651 | 53.309 | 559 | 179 | 14 | 927 | 1413 | 773 | 1321 | 4.44e-138 | 461 |
| Msa0583540 | MtrunA17_Chr3g0085651 | 52.847 | 562 | 162 | 14 | 837 | 1305 | 773 | 1324 | 1.19e-136 | 457 |
| Msa0583540 | MtrunA17_Chr3g0085651 | 52.143 | 560 | 169 | 13 | 882 | 1350 | 773 | 1324 | 1.23e-133 | 448 |
| Msa0583540 | MtrunA17_Chr3g0086161 | 70.347 | 1123 | 272 | 17 | 1 | 1074 | 1 | 1111 | 0.0 | 1441 |
| Msa0583540 | MtrunA17_Chr3g0086161 | 61.538 | 260 | 89 | 3 | 1287 | 1536 | 765 | 1023 | 2.47e-81 | 293 |
| Msa0583540 | MtrunA17_Chr3g0086161 | 35.574 | 357 | 191 | 15 | 1017 | 1344 | 765 | 1111 | 1.88e-26 | 118 |
| Msa0583540 | MtrunA17_Chr3g0086161 | 30.390 | 385 | 198 | 16 | 1197 | 1537 | 765 | 1123 | 1.35e-21 | 102 |
| Msa0583540 | MtrunA17_Chr3g0086161 | 31.486 | 397 | 184 | 16 | 837 | 1191 | 765 | 1115 | 6.00e-21 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0583540 | AT3G14470.1 | 35.340 | 1030 | 555 | 21 | 5 | 944 | 4 | 1012 | 7.36e-174 | 550 |
| Msa0583540 | AT3G14470.2 | 35.340 | 1030 | 555 | 21 | 5 | 944 | 4 | 1012 | 7.36e-174 | 550 |
| Msa0583540 | AT3G14460.1 | 33.586 | 923 | 534 | 19 | 31 | 893 | 32 | 935 | 6.02e-138 | 462 |
| Msa0583540 | AT3G50950.2 | 28.143 | 867 | 539 | 32 | 8 | 830 | 4 | 830 | 1.03e-66 | 244 |
| Msa0583540 | AT3G50950.1 | 28.143 | 867 | 539 | 32 | 8 | 830 | 4 | 830 | 1.03e-66 | 244 |
| Msa0583540 | AT1G59124.2 | 27.590 | 975 | 599 | 38 | 35 | 937 | 31 | 970 | 3.47e-63 | 236 |
| Msa0583540 | AT1G58807.1 | 27.590 | 975 | 599 | 38 | 35 | 937 | 31 | 970 | 3.47e-63 | 236 |
| Msa0583540 | AT1G58602.2 | 26.188 | 1115 | 691 | 44 | 35 | 1063 | 31 | 1099 | 1.90e-62 | 234 |
| Msa0583540 | AT1G58602.2 | 31.650 | 297 | 178 | 12 | 1108 | 1387 | 823 | 1111 | 1.74e-18 | 92.8 |
| Msa0583540 | AT1G58602.2 | 32.246 | 276 | 170 | 9 | 1018 | 1281 | 823 | 1093 | 1.99e-18 | 92.8 |
Find 275 sgRNAs with CRISPR-Local
Find 382 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTGATTGGTTCGAGAG+AGG | 0.627248 | 4_2:+2249211 | Msa0583540:CDS |
| AAAACTTCTTGCAGAGCAAA+AGG | 0.461919 | 4_2:+2244978 | Msa0583540:CDS |
| AAACAACGCTTTAGATTGGA+AGG | 0.544047 | 4_2:-2245849 | None:intergenic |
| AAACACTTTCAATAAAAGGA+TGG | 0.555454 | 4_2:+2249048 | Msa0583540:CDS |
| AAAGAGATGTGATAGCATTT+TGG | 0.262558 | 4_2:+2248854 | Msa0583540:CDS |
| AAATGAATTGCCGACCAGCT+TGG | 0.524020 | 4_2:+2248878 | Msa0583540:CDS |
| AAATGTACAACGAAAGAGCA+AGG | 0.636482 | 4_2:+2244888 | Msa0583540:CDS |
| AACAACGCTTTAGATTGGAA+GGG | 0.666966 | 4_2:-2245848 | None:intergenic |
| AACAAGCAGAAATTTCTTTC+CGG | 0.271291 | 4_2:-2245406 | None:intergenic |
| AACCAAATAGCAACTTGAAG+AGG | 0.515741 | 4_2:+2246834 | Msa0583540:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAAGCAGAAATTTCTTTC+CGG | - | chr4_2:2245409-2245428 | None:intergenic | 30.0% | |
| TATCCAAATCTTGAACCAAT+TGG | + | chr4_2:2245648-2245667 | Msa0583540:CDS | 30.0% | |
| AAGAAGATTGTAGAGAAATG+TGG | + | chr4_2:2245672-2245691 | Msa0583540:CDS | 30.0% | |
| CTTGGGAAATATTGAACAAT+AGG | - | chr4_2:2245877-2245896 | None:intergenic | 30.0% | |
| CTATTGTTCAATATTTCCCA+AGG | + | chr4_2:2245875-2245894 | Msa0583540:CDS | 30.0% | |
| AAGATGAATTGATCAAGCTT+TGG | + | chr4_2:2245913-2245932 | Msa0583540:CDS | 30.0% | |
| TATGATCATCTAGTGATTGT+TGG | - | chr4_2:2246034-2246053 | None:intergenic | 30.0% | |
| AATCACTAGATGATCATAAG+AGG | + | chr4_2:2246036-2246055 | Msa0583540:CDS | 30.0% | |
| AATTTGTAAGCAAAACCCTT+GGG | - | chr4_2:2246105-2246124 | None:intergenic | 30.0% | |
| TGCTCTCTTAATTTCAATGA+TGG | + | chr4_2:2246173-2246192 | Msa0583540:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 2244634 | 2249259 | 2244634 | ID=Msa0583540;Name=Msa0583540 |
| chr4_2 | mRNA | 2244634 | 2249259 | 2244634 | ID=Msa0583540-mRNA-1;Parent=Msa0583540;Name=Msa0583540-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|1541 |
| chr4_2 | exon | 2244634 | 2249259 | 2244634 | ID=Msa0583540-mRNA-1:exon:595;Parent=Msa0583540-mRNA-1 |
| chr4_2 | CDS | 2244634 | 2249259 | 2244634 | ID=Msa0583540-mRNA-1:cds;Parent=Msa0583540-mRNA-1 |
| Gene Sequence |
>Msa0583540 ATGGCAGAGCTGATTGGTGGAGCATTTCTTTCGTCATTCTTTCAAGTCACTCTTCAAAGCTTAACCTTAAGAGATTTCAAAGACCTCTTCAATAAAAAGTTGATTAATAAACTTGAAATCACACTGAATTCTATCAATCAATTGTTGGATGATGCTGAGACAAAGAAGTACCATAACCAAAATGTGAAGAATTGGCTTGATCGTCTAAAGCACGAGGTATATGAAGTAGATCAATTGTTGGATGAGTTTGATACAAATGTACAACGAAAGAGCAAGGTTCAACACTTCCTTTCAGCTTTCACTAATCGGTTTGAATCTAGGATCAAAGATTTGCTAGATGAGCTAAAACTTCTTGCAGAGCAAAAGGATGTGTTGGGATTGACACAAAGAAGTTGCACTAGTAATGAAGGTGCAGTTAGCCTGCAATCCTCAAAAAGATTGCCAACTGCATCTTTGGTGGATGAATCTTGCATATATGGTAGAGAGGCTAACAAAGAAGAAATAATCAACTATTTGCTTTCATGTAAAGACAACAGCAACCAGGTATCAACAATCAGCATAGTAGGTTTAGGTGGGATGGGAAAGACCACCCTTGCACAACTTGTCTACAATGACCAGAGGATGGAGAAGCATTTTGAACTTAAAGCTTGGGTCCATGTCTCAAAATCTTTTGATGTTGTTGGACTCACAAAAACAATTCTCAGGTCATTCCATTCTCCTGCAGACGGTGAAGACTTGGATCCACTCATTTGTCAACTACAAAAGACACTAACCGGAAAGAAATTTCTGCTTGTTCTAGATGATGTGTGGAATGGGAATGAGGAATGTTTGGAGCAATTACTACTTCCTTTTAACACTGGACTTTTAGGAAGTAAGATTATTGTGACAACACGTGACAAACATGTTGCATTTGTCATGAAATCTGACCATCAACTTCTTTTAGAACAATTGGAGGAGAAAGATTGTTGGAGTATATTTGTGAAACATGCTTTTCGAGGAAAAAATGTGTTTGAATATCCAAATCTTGAACCAATTGGCAAGAAGATTGTAGAGAAATGTGGAGGGTTGCCTCTAGCTGTGAAAATGTTGGGGAATCTCTCGCAAAGAAAATTTTCTGAAGCTGATTGGTGTAAGATATTGGAGACCGATATGTGGCATGTATCGAAAGGTGGTGATGAAATTAACTCAGTATTGAGATTGAGTTACCATAATCTCCCTTCCAATCTAAAGCGTTGTTTTGCCTATTGTTCAATATTTCCCAAGGGCTATGTATTTGAAAAAGATGAATTGATCAAGCTTTGGATGGCAGAAGGTTTGCTGAAATGTTGCGGAAGAGACAAAACTGAAGAAGAGTTGGGTAATGAATTCTTTTATGATCTAGAGTCCATTTCATTTTTCCAACAATCACTAGATGATCATAAGAGGATGGTCATGCATGATCTTGTCAATGATTTGGCAAAATCAGAGTCCCAAGGGTTTTGCTTACAAATTGAGGGTGATAGTGTGCAAGATAAATCTAAAAGAACACGACACGTTTGCTGCTCTCTTAATTTCAATGATGGTGCGGGAATATTGAATCACATTTCTAAGATCAATGGATTACGCAGCTTGTTGGTATTACGACGAGGCTATTTCATTATAAGCAACAATTTGCAACGTGATCTATTTTCAAAACTAAAATATTTGCGGATGTTGTCGTTATGTGGTTGTGAACTCAGAGAGCTATCAGGCGAGGTAGGAAATTTAGAGCTTTTGCGCTATCTGAACCTGGCTAAAACATTAATTGAAAGGTTGCCTGACTCAATTTATAAGCTGTATAAATTAGAGACACTGATACTGGAAGACTGTTTTAAATTGACTAAACTCCCTTCAAAATTTTACAAACTTGTGAGTTTGCGTCATCTTAATCTCAAAGGCTGTAAAATAAAGACAATGCCAACACAGATAGGGAGTTTAAACCATCTTCAAACGCTGAGTGATTTTGTTGTCGGAGAGGAGAATGGGTCTAATATTCAGGAGTTGGGTAATCTCAATCGTCTTCAAGGGAAACTTTGTATTTCAGGATTGGAACATGTCGTTAATCCTGAAGATGCCGCAGGAGCAAATTTGAAAGATAAGAAACATGTAGAAGAATTATATATGAATTATGGTGACAAATTCAACAACAATGGAAGAGAACTGGATGTCTTTGAGGCTCTTCAACCAAATAGCAACTTGAAGAGGCTTTACATCAAAAGATACAAAGGTATTAGCTTTGCAAATTGGATAAGCGGTTGTCATTTACCAAACTTAGTATCTTTTCAATTGCAGAGCTGTGATAGGATAAGGATCATAGGGAAAGAATTTTATGGCAATAATTCAACAAATGTACCATTCAGATCCCTTGAAGTTCTGCGTTTTGAATCGATGTTGAATTGGGAGGAATGGTCATGTCTTGAAGGGTTTCCTATGCTTAAGGAGCTTTGTATAGAATATTGTCCCGAATTGAAAAGGGCCTTGCTACCTCAACACCTTCCTTCTTTGCAAAAATTGGAGATTAGTGGTTGCAACAAATTGGACGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCAATTGTCCCGAATTGAAAAGGGCTCTGCCACAACGCCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCGATTGTCCCGAATTGAAAAGGGCTCTGCCTCAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCAATTGTCCCGAATTGAAAAGGGCTCTGCCACAATGCCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCGATTGTCCCGAATTGAAAAGGGCTCTGCCTCAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCGATTGTCCCGAATTGAAAAGGGCTCTGCCTGAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCAATTGTCCCGAATTGAAAAGGGCTCTGCCTCAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAATTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCAATTGTCCCGAATTGAAAAGGACTCTGCCACAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCGATTGTCCCGAATTGAAAAGGGCTCTGCCTCAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCAATTGTCCCGAATTGAAAAGGGCTCTGCCTCAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAATTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCAATTGTCCCGAATTGAAAAGGACTCTGCCACAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCGATTGTCCCGAATTGAAAAGGGCTCTGCCTCAACACCTTCCTTCTTTACAAAAACTGGAGATTAGTAATTGCAACAAGTTGGAGGAATTGTTATGCCTTGGAGAGTTTCCTTTGCTTAAAGAGATTTCTATATCCGATTGTCCCGAATTGAAATGGGCTCTGCCACAATACCTTCCTTCTTTACAAAAACTGTTTATTAGTGATTGCAACAAGTTGGAGGCATCAATTCCCAAGGGTGATAATATGATAGAGTTAGATATAAAGAGATGTGATAGCATTTTGGTAAATGAATTGCCGACCAGCTTGGAAAAGTTAGTCATTGATGAAAATCGGCACACAGAGTTCTCCGTGGAGCAGACTTTCGTCAACAATACCATTCTTGAAGAGTTAAAGTTGGATTTTAGAGACTGTGTAAATTGTCCCTCTTTGGATTTGCATTGCTATGATTCTCTTAAAACACTTTCAATAAAAGGATGGCGCTCCTCTTCCTTGCCTTTTTCACTAAACTTGTTCACCAGGCTTCGTACTCTGAATCTGTACGATTGTCCAGAGCTGGAAACGTTTCAAATAGGTGGTTTGCCTTCCAACTTGAGGAGCCTTGGAATATACGATTGCCCAAAACTGATTGGTTCGAGAGAGGAGTGGGGTTCCAACTCAATTCCTTGA |
| Protein sequence |
>Msa0583540 MAELIGGAFLSSFFQVTLQSLTLRDFKDLFNKKLINKLEITLNSINQLLDDAETKKYHNQNVKNWLDRLKHEVYEVDQLLDEFDTNVQRKSKVQHFLSAFTNRFESRIKDLLDELKLLAEQKDVLGLTQRSCTSNEGAVSLQSSKRLPTASLVDESCIYGREANKEEIINYLLSCKDNSNQVSTISIVGLGGMGKTTLAQLVYNDQRMEKHFELKAWVHVSKSFDVVGLTKTILRSFHSPADGEDLDPLICQLQKTLTGKKFLLVLDDVWNGNEECLEQLLLPFNTGLLGSKIIVTTRDKHVAFVMKSDHQLLLEQLEEKDCWSIFVKHAFRGKNVFEYPNLEPIGKKIVEKCGGLPLAVKMLGNLSQRKFSEADWCKILETDMWHVSKGGDEINSVLRLSYHNLPSNLKRCFAYCSIFPKGYVFEKDELIKLWMAEGLLKCCGRDKTEEELGNEFFYDLESISFFQQSLDDHKRMVMHDLVNDLAKSESQGFCLQIEGDSVQDKSKRTRHVCCSLNFNDGAGILNHISKINGLRSLLVLRRGYFIISNNLQRDLFSKLKYLRMLSLCGCELRELSGEVGNLELLRYLNLAKTLIERLPDSIYKLYKLETLILEDCFKLTKLPSKFYKLVSLRHLNLKGCKIKTMPTQIGSLNHLQTLSDFVVGEENGSNIQELGNLNRLQGKLCISGLEHVVNPEDAAGANLKDKKHVEELYMNYGDKFNNNGRELDVFEALQPNSNLKRLYIKRYKGISFANWISGCHLPNLVSFQLQSCDRIRIIGKEFYGNNSTNVPFRSLEVLRFESMLNWEEWSCLEGFPMLKELCIEYCPELKRALLPQHLPSLQKLEISGCNKLDELLCLGEFPLLKEISISNCPELKRALPQRLPSLQKLEISNCNKLEELLCLGEFPLLKEISISDCPELKRALPQHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISNCPELKRALPQCLPSLQKLEISNCNKLEELLCLGEFPLLKEISISDCPELKRALPQHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISDCPELKRALPEHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISNCPELKRALPQHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISNCPELKRTLPQHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISDCPELKRALPQHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISNCPELKRALPQHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISNCPELKRTLPQHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISDCPELKRALPQHLPSLQKLEISNCNKLEELLCLGEFPLLKEISISDCPELKWALPQYLPSLQKLFISDCNKLEASIPKGDNMIELDIKRCDSILVNELPTSLEKLVIDENRHTEFSVEQTFVNNTILEELKLDFRDCVNCPSLDLHCYDSLKTLSIKGWRSSSLPFSLNLFTRLRTLNLYDCPELETFQIGGLPSNLRSLGIYDCPKLIGSREEWGSNSIP* |
