Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0614120 | RHN62674.1 | 98.667 | 75 | 1 | 0 | 1 | 75 | 76 | 150 | 3.65e-44 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0560070 | Msa0614120 | 0.833016 | 6.793843e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0614120 | MtrunA17_Chr4g0049961 | 98.667 | 75 | 1 | 0 | 1 | 75 | 76 | 150 | 3.36e-48 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGATTTGGGCAAAATTTC+AGG | 0.085844 | 4_2:+63040952 | Msa0614120:CDS |
| GACACAAACATTTCCTGATT+TGG | 0.225196 | 4_2:+63040939 | Msa0614120:CDS |
| GAAGTCAGGAATATTGAAAA+TGG | 0.251652 | 4_2:-63041034 | None:intergenic |
| ACACAAACATTTCCTGATTT+GGG | 0.316697 | 4_2:+63040940 | Msa0614120:CDS |
| CTGATTTGGGCAAAATTTCA+GGG | 0.443347 | 4_2:+63040953 | Msa0614120:CDS |
| TACTCTGGCCATTCAGTCAC+TGG | 0.448656 | 4_2:-63040997 | None:intergenic |
| TCCTTGGACATTGTCAATGC+TGG | 0.458633 | 4_2:-63040896 | None:intergenic |
| ATTGACAATGTCCAAGGAGG+TGG | 0.459649 | 4_2:+63040901 | Msa0614120:CDS |
| CTTTCCCACCAGTGACTGAA+TGG | 0.466160 | 4_2:+63040989 | Msa0614120:CDS |
| GATGGTGGAGAGAAGAAGTC+AGG | 0.493659 | 4_2:-63041048 | None:intergenic |
| AAGTCAGGAATATTGAAAAT+GGG | 0.495802 | 4_2:-63041033 | None:intergenic |
| TGACTGAATGGCCAGAGTAC+AGG | 0.500741 | 4_2:+63041001 | Msa0614120:CDS |
| CATTCAGTCACTGGTGGGAA+AGG | 0.519050 | 4_2:-63040988 | None:intergenic |
| GGTGGAGGCAACCTGTACTC+TGG | 0.553702 | 4_2:-63041012 | None:intergenic |
| GTTGTTGTGGTGGCGTCAGA+TGG | 0.566737 | 4_2:-63041066 | None:intergenic |
| TCAGTCACTGGTGGGAAAGG+AGG | 0.574402 | 4_2:-63040985 | None:intergenic |
| TCCAGCATTGACAATGTCCA+AGG | 0.589594 | 4_2:+63040895 | Msa0614120:CDS |
| TCTGGCCATTCAGTCACTGG+TGG | 0.608143 | 4_2:-63040994 | None:intergenic |
| TCAGGAATATTGAAAATGGG+TGG | 0.625430 | 4_2:-63041030 | None:intergenic |
| TTGACAATGTCCAAGGAGGT+GGG | 0.628153 | 4_2:+63040902 | Msa0614120:CDS |
| CTGGCCATTCAGTCACTGGT+GGG | 0.629336 | 4_2:-63040993 | None:intergenic |
| GTTGTGGTGGCGTCAGATGG+TGG | 0.629556 | 4_2:-63041063 | None:intergenic |
| GGAATATTGAAAATGGGTGG+AGG | 0.630069 | 4_2:-63041027 | None:intergenic |
| AGCATTGACAATGTCCAAGG+AGG | 0.730109 | 4_2:+63040898 | Msa0614120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAGCTAACAAAAACAAA+CGG | - | chr4_2:63040858-63040877 | None:intergenic | 20.0% |
| ! | AAGTCAGGAATATTGAAAAT+GGG | - | chr4_2:63041036-63041055 | None:intergenic | 25.0% |
| ACACAAACATTTCCTGATTT+GGG | + | chr4_2:63040940-63040959 | Msa0614120:CDS | 30.0% | |
| GAAGTCAGGAATATTGAAAA+TGG | - | chr4_2:63041037-63041056 | None:intergenic | 30.0% | |
| TAACAAAAACAAACGGACAG+AGG | - | chr4_2:63040851-63040870 | None:intergenic | 35.0% | |
| GACACAAACATTTCCTGATT+TGG | + | chr4_2:63040939-63040958 | Msa0614120:CDS | 35.0% | |
| !! | CTGATTTGGGCAAAATTTCA+GGG | + | chr4_2:63040953-63040972 | Msa0614120:CDS | 35.0% |
| TCAGGAATATTGAAAATGGG+TGG | - | chr4_2:63041033-63041052 | None:intergenic | 35.0% | |
| ! | CCTGAAATTTTGCCCAAATC+AGG | - | chr4_2:63040955-63040974 | None:intergenic | 40.0% |
| !! | CCTGATTTGGGCAAAATTTC+AGG | + | chr4_2:63040952-63040971 | Msa0614120:CDS | 40.0% |
| GGAATATTGAAAATGGGTGG+AGG | - | chr4_2:63041030-63041049 | None:intergenic | 40.0% | |
| ! | TTTTGTAGCAGTTGTTGTGG+TGG | - | chr4_2:63041079-63041098 | None:intergenic | 40.0% |
| !!! | TGGTTTTGTAGCAGTTGTTG+TGG | - | chr4_2:63041082-63041101 | None:intergenic | 40.0% |
| !! | TCCAGCATTGACAATGTCCA+AGG | + | chr4_2:63040895-63040914 | Msa0614120:CDS | 45.0% |
| TCCTTGGACATTGTCAATGC+TGG | - | chr4_2:63040899-63040918 | None:intergenic | 45.0% | |
| !! | AGCATTGACAATGTCCAAGG+AGG | + | chr4_2:63040898-63040917 | Msa0614120:CDS | 45.0% |
| ATTGACAATGTCCAAGGAGG+TGG | + | chr4_2:63040901-63040920 | Msa0614120:CDS | 45.0% | |
| TTGACAATGTCCAAGGAGGT+GGG | + | chr4_2:63040902-63040921 | Msa0614120:CDS | 45.0% | |
| !! | GTTTTCTAGTCCCACCTCCT+TGG | - | chr4_2:63040915-63040934 | None:intergenic | 50.0% |
| CATTCAGTCACTGGTGGGAA+AGG | - | chr4_2:63040991-63041010 | None:intergenic | 50.0% | |
| CTTTCCCACCAGTGACTGAA+TGG | + | chr4_2:63040989-63041008 | Msa0614120:CDS | 50.0% | |
| TACTCTGGCCATTCAGTCAC+TGG | - | chr4_2:63041000-63041019 | None:intergenic | 50.0% | |
| TGACTGAATGGCCAGAGTAC+AGG | + | chr4_2:63041001-63041020 | Msa0614120:CDS | 50.0% | |
| !! | GATGGTGGAGAGAAGAAGTC+AGG | - | chr4_2:63041051-63041070 | None:intergenic | 50.0% |
| TCAGTCACTGGTGGGAAAGG+AGG | - | chr4_2:63040988-63041007 | None:intergenic | 55.0% | |
| CTGGCCATTCAGTCACTGGT+GGG | - | chr4_2:63040996-63041015 | None:intergenic | 55.0% | |
| TCTGGCCATTCAGTCACTGG+TGG | - | chr4_2:63040997-63041016 | None:intergenic | 55.0% | |
| ! | GTTGTTGTGGTGGCGTCAGA+TGG | - | chr4_2:63041069-63041088 | None:intergenic | 55.0% |
| GGTGGAGGCAACCTGTACTC+TGG | - | chr4_2:63041015-63041034 | None:intergenic | 60.0% | |
| ! | GTTGTGGTGGCGTCAGATGG+TGG | - | chr4_2:63041066-63041085 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 63040844 | 63041104 | 63040844 | ID=Msa0614120;Name=Msa0614120 |
| chr4_2 | mRNA | 63040844 | 63041104 | 63040844 | ID=Msa0614120-mRNA-1;Parent=Msa0614120;Name=Msa0614120-mRNA-1;_AED=0.19;_eAED=0.20;_QI=0|-1|0|1|-1|1|1|0|86 |
| chr4_2 | exon | 63040844 | 63041104 | 63040844 | ID=Msa0614120-mRNA-1:exon:17505;Parent=Msa0614120-mRNA-1 |
| chr4_2 | CDS | 63040844 | 63041104 | 63040844 | ID=Msa0614120-mRNA-1:cds;Parent=Msa0614120-mRNA-1 |
| Gene Sequence |
| Protein sequence |
