Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0614170 | Msa0638650 | 0.840953 | 6.372967e-58 | -8.615850e-47 |
| Msa0614170 | Msa0697350 | 0.818015 | 2.444106e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAATAGATTCACCGTTGC+CGG | 0.314871 | 4_2:-63091177 | None:intergenic |
| TGGAGTGGCAACGGGAGATT+TGG | 0.322110 | 4_2:+63091404 | Msa0614170:CDS |
| TCTCCTCCTCCTGCTTCCTC+TGG | 0.367416 | 4_2:-63091279 | None:intergenic |
| AGGAGATGGTGGAGAGTTGA+TGG | 0.371781 | 4_2:+63091296 | Msa0614170:CDS |
| AGTGACAGCAGGGGCGGCGG+CGG | 0.427837 | 4_2:+63091251 | Msa0614170:CDS |
| ATCGATGATGGAGTGACAGC+AGG | 0.443446 | 4_2:+63091240 | Msa0614170:CDS |
| GAAGCAGGAGGAGGAGATGG+TGG | 0.444045 | 4_2:+63091285 | Msa0614170:CDS |
| GCGGGAGATCCAGAGGAAGC+AGG | 0.447382 | 4_2:+63091270 | Msa0614170:CDS |
| GTGACAGCAGGGGCGGCGGC+GGG | 0.450586 | 4_2:+63091252 | Msa0614170:CDS |
| CCGTTCGATGGTGCTTCAGC+AGG | 0.467052 | 4_2:+63091207 | Msa0614170:CDS |
| GCAGGAGATGGAGTGGCAAC+GGG | 0.467415 | 4_2:+63091396 | Msa0614170:CDS |
| CTCTCCCTCCGCTGACTCAC+CGG | 0.481791 | 4_2:-63091370 | None:intergenic |
| CGCAGGAGATGGAGTGGCAA+CGG | 0.487389 | 4_2:+63091395 | Msa0614170:CDS |
| TGGAGTGACAGCAGGGGCGG+CGG | 0.489307 | 4_2:+63091248 | Msa0614170:CDS |
| AATCACAACAGCAGCAGATC+CGG | 0.489743 | 4_2:+63091158 | Msa0614170:CDS |
| GGAGCGTTCTTCACAGGAAT+GGG | 0.492077 | 4_2:+63091336 | Msa0614170:CDS |
| GAGGAAGCAGGAGGAGGAGA+TGG | 0.494965 | 4_2:+63091282 | Msa0614170:CDS |
| GGAGGAGTGGAGATCGATGA+TGG | 0.496417 | 4_2:+63091228 | Msa0614170:CDS |
| GGAGATGGAGCGTTCTTCAC+AGG | 0.497278 | 4_2:+63091330 | Msa0614170:CDS |
| TTCAAAGCGGCGCCGTTCGA+TGG | 0.497857 | 4_2:+63091195 | Msa0614170:CDS |
| TCAGCGGAGGGAGAGATCGC+AGG | 0.499814 | 4_2:+63091378 | Msa0614170:CDS |
| GAGGGAGAGATCGCAGGAGA+TGG | 0.500935 | 4_2:+63091384 | Msa0614170:CDS |
| TGGAGCGTTCTTCACAGGAA+TGG | 0.509007 | 4_2:+63091335 | Msa0614170:CDS |
| GATCCAGAGGAAGCAGGAGG+AGG | 0.530807 | 4_2:+63091276 | Msa0614170:CDS |
| TTCGATGGTGCTTCAGCAGG+AGG | 0.539403 | 4_2:+63091210 | Msa0614170:CDS |
| GGCGGCGGCGGGAGATCCAG+AGG | 0.539861 | 4_2:+63091263 | Msa0614170:CDS |
| ATGGTGGATGGTGAAGGAGA+TGG | 0.550636 | 4_2:+63091315 | Msa0614170:CDS |
| TGCCGCCGGTGAGTCAGCGG+AGG | 0.562517 | 4_2:+63091365 | Msa0614170:CDS |
| TCGATGATGGAGTGACAGCA+GGG | 0.563807 | 4_2:+63091241 | Msa0614170:CDS |
| AGAGATCGCAGGAGATGGAG+TGG | 0.573319 | 4_2:+63091389 | Msa0614170:CDS |
| AGATGCCGCCGGTGAGTCAG+CGG | 0.584628 | 4_2:+63091362 | Msa0614170:CDS |
| GGAGATCCAGAGGAAGCAGG+AGG | 0.586852 | 4_2:+63091273 | Msa0614170:CDS |
| GTGGCAACGGGAGATTTGGA+TGG | 0.591213 | 4_2:+63091408 | Msa0614170:CDS |
| AGATGGTGGAGAGTTGATGG+TGG | 0.602459 | 4_2:+63091299 | Msa0614170:CDS |
| TGATGGAGTGACAGCAGGGG+CGG | 0.602853 | 4_2:+63091245 | Msa0614170:CDS |
| TGGTGCTTCAGCAGGAGGAG+TGG | 0.604768 | 4_2:+63091215 | Msa0614170:CDS |
| GGTGGAGAGTTGATGGTGGA+TGG | 0.609584 | 4_2:+63091303 | Msa0614170:CDS |
| CCTGCTGAAGCACCATCGAA+CGG | 0.619717 | 4_2:-63091207 | None:intergenic |
| GAGTTGATGGTGGATGGTGA+AGG | 0.628457 | 4_2:+63091309 | Msa0614170:CDS |
| AACAGCAGCAGATCCGGCAA+CGG | 0.637005 | 4_2:+63091164 | Msa0614170:CDS |
| GCCGCCGGTGAGTCAGCGGA+GGG | 0.648009 | 4_2:+63091366 | Msa0614170:CDS |
| CGATGATGGAGTGACAGCAG+GGG | 0.655107 | 4_2:+63091242 | Msa0614170:CDS |
| AACGGTGAATCTATTCAAAG+CGG | 0.675574 | 4_2:+63091182 | Msa0614170:CDS |
| TCCCTCCGCTGACTCACCGG+CGG | 0.684106 | 4_2:-63091367 | None:intergenic |
| TCTCTACATCATCAAAGCGG+CGG | 0.692163 | 4_2:+63091131 | Msa0614170:CDS |
| AACTCTCTACATCATCAAAG+CGG | 0.713170 | 4_2:+63091128 | Msa0614170:CDS |
| GGAATGGGAGAAGATGCCGC+CGG | 0.729198 | 4_2:+63091351 | Msa0614170:CDS |
| GATTTGGATGGAGATGACGA+TGG | 0.743905 | 4_2:+63091420 | Msa0614170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAACAGAGCAACAACAAAA+AGG | + | chr4_2:63091027-63091046 | Msa0614170:intron | 35.0% | |
| TTGTTAATCACGCATTTACG+CGG | - | chr4_2:63091070-63091089 | None:intergenic | 35.0% | |
| AACTCTCTACATCATCAAAG+CGG | + | chr4_2:63091128-63091147 | Msa0614170:CDS | 35.0% | |
| AACGGTGAATCTATTCAAAG+CGG | + | chr4_2:63091182-63091201 | Msa0614170:CDS | 35.0% | |
| TTGAATAGATTCACCGTTGC+CGG | - | chr4_2:63091180-63091199 | None:intergenic | 40.0% | |
| TCTCTACATCATCAAAGCGG+CGG | + | chr4_2:63091131-63091150 | Msa0614170:CDS | 45.0% | |
| AATCACAACAGCAGCAGATC+CGG | + | chr4_2:63091158-63091177 | Msa0614170:CDS | 45.0% | |
| GATTTGGATGGAGATGACGA+TGG | + | chr4_2:63091420-63091439 | Msa0614170:CDS | 45.0% | |
| !! | ATCGATGATGGAGTGACAGC+AGG | + | chr4_2:63091240-63091259 | Msa0614170:CDS | 50.0% |
| !! | TCGATGATGGAGTGACAGCA+GGG | + | chr4_2:63091241-63091260 | Msa0614170:CDS | 50.0% |
| !! | AGGAGATGGTGGAGAGTTGA+TGG | + | chr4_2:63091296-63091315 | Msa0614170:CDS | 50.0% |
| !! | AGATGGTGGAGAGTTGATGG+TGG | + | chr4_2:63091299-63091318 | Msa0614170:CDS | 50.0% |
| !! | GAGTTGATGGTGGATGGTGA+AGG | + | chr4_2:63091309-63091328 | Msa0614170:CDS | 50.0% |
| !! | ATGGTGGATGGTGAAGGAGA+TGG | + | chr4_2:63091315-63091334 | Msa0614170:CDS | 50.0% |
| ! | TGGAGCGTTCTTCACAGGAA+TGG | + | chr4_2:63091335-63091354 | Msa0614170:CDS | 50.0% |
| ! | GGAGCGTTCTTCACAGGAAT+GGG | + | chr4_2:63091336-63091355 | Msa0614170:CDS | 50.0% |
| AACAGCAGCAGATCCGGCAA+CGG | + | chr4_2:63091164-63091183 | Msa0614170:CDS | 55.0% | |
| ! | CCTGCTGAAGCACCATCGAA+CGG | - | chr4_2:63091210-63091229 | None:intergenic | 55.0% |
| TTCGATGGTGCTTCAGCAGG+AGG | + | chr4_2:63091210-63091229 | Msa0614170:CDS | 55.0% | |
| GGAGGAGTGGAGATCGATGA+TGG | + | chr4_2:63091228-63091247 | Msa0614170:CDS | 55.0% | |
| !! | CGATGATGGAGTGACAGCAG+GGG | + | chr4_2:63091242-63091261 | Msa0614170:CDS | 55.0% |
| !! | GGTGGAGAGTTGATGGTGGA+TGG | + | chr4_2:63091303-63091322 | Msa0614170:CDS | 55.0% |
| ! | GGAGATGGAGCGTTCTTCAC+AGG | + | chr4_2:63091330-63091349 | Msa0614170:CDS | 55.0% |
| AGAGATCGCAGGAGATGGAG+TGG | + | chr4_2:63091389-63091408 | Msa0614170:CDS | 55.0% | |
| TGGAGTGGCAACGGGAGATT+TGG | + | chr4_2:63091404-63091423 | Msa0614170:CDS | 55.0% | |
| GTGGCAACGGGAGATTTGGA+TGG | + | chr4_2:63091408-63091427 | Msa0614170:CDS | 55.0% | |
| TTCAAAGCGGCGCCGTTCGA+TGG | + | chr4_2:63091195-63091214 | Msa0614170:CDS | 60.0% | |
| CCGTTCGATGGTGCTTCAGC+AGG | + | chr4_2:63091207-63091226 | Msa0614170:CDS | 60.0% | |
| TGGTGCTTCAGCAGGAGGAG+TGG | + | chr4_2:63091215-63091234 | Msa0614170:CDS | 60.0% | |
| TGATGGAGTGACAGCAGGGG+CGG | + | chr4_2:63091245-63091264 | Msa0614170:CDS | 60.0% | |
| GGAGATCCAGAGGAAGCAGG+AGG | + | chr4_2:63091273-63091292 | Msa0614170:CDS | 60.0% | |
| GATCCAGAGGAAGCAGGAGG+AGG | + | chr4_2:63091276-63091295 | Msa0614170:CDS | 60.0% | |
| TCTCCTCCTCCTGCTTCCTC+TGG | - | chr4_2:63091282-63091301 | None:intergenic | 60.0% | |
| GAGGAAGCAGGAGGAGGAGA+TGG | + | chr4_2:63091282-63091301 | Msa0614170:CDS | 60.0% | |
| GAAGCAGGAGGAGGAGATGG+TGG | + | chr4_2:63091285-63091304 | Msa0614170:CDS | 60.0% | |
| GGAATGGGAGAAGATGCCGC+CGG | + | chr4_2:63091351-63091370 | Msa0614170:CDS | 60.0% | |
| GAGGGAGAGATCGCAGGAGA+TGG | + | chr4_2:63091384-63091403 | Msa0614170:CDS | 60.0% | |
| CGCAGGAGATGGAGTGGCAA+CGG | + | chr4_2:63091395-63091414 | Msa0614170:CDS | 60.0% | |
| GCAGGAGATGGAGTGGCAAC+GGG | + | chr4_2:63091396-63091415 | Msa0614170:CDS | 60.0% | |
| GCGGGAGATCCAGAGGAAGC+AGG | + | chr4_2:63091270-63091289 | Msa0614170:CDS | 65.0% | |
| AGATGCCGCCGGTGAGTCAG+CGG | + | chr4_2:63091362-63091381 | Msa0614170:CDS | 65.0% | |
| !! | CTCTCCCTCCGCTGACTCAC+CGG | - | chr4_2:63091373-63091392 | None:intergenic | 65.0% |
| TCAGCGGAGGGAGAGATCGC+AGG | + | chr4_2:63091378-63091397 | Msa0614170:CDS | 65.0% | |
| TGGAGTGACAGCAGGGGCGG+CGG | + | chr4_2:63091248-63091267 | Msa0614170:CDS | 70.0% | |
| !! | TCCCTCCGCTGACTCACCGG+CGG | - | chr4_2:63091370-63091389 | None:intergenic | 70.0% |
| AGTGACAGCAGGGGCGGCGG+CGG | + | chr4_2:63091251-63091270 | Msa0614170:CDS | 75.0% | |
| TGCCGCCGGTGAGTCAGCGG+AGG | + | chr4_2:63091365-63091384 | Msa0614170:CDS | 75.0% | |
| GCCGCCGGTGAGTCAGCGGA+GGG | + | chr4_2:63091366-63091385 | Msa0614170:CDS | 75.0% | |
| ! | GTGACAGCAGGGGCGGCGGC+GGG | + | chr4_2:63091252-63091271 | Msa0614170:CDS | 80.0% |
| ! | GGCGGCGGCGGGAGATCCAG+AGG | + | chr4_2:63091263-63091282 | Msa0614170:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 63090922 | 63091449 | 63090922 | ID=Msa0614170;Name=Msa0614170 |
| chr4_2 | mRNA | 63090922 | 63091449 | 63090922 | ID=Msa0614170-mRNA-1;Parent=Msa0614170;Name=Msa0614170-mRNA-1;_AED=0.42;_eAED=1.00;_QI=0|0|0|0.5|1|1|2|0|110 |
| chr4_2 | exon | 63090922 | 63090929 | 63090922 | ID=Msa0614170-mRNA-1:exon:17543;Parent=Msa0614170-mRNA-1 |
| chr4_2 | exon | 63091125 | 63091449 | 63091125 | ID=Msa0614170-mRNA-1:exon:17544;Parent=Msa0614170-mRNA-1 |
| chr4_2 | CDS | 63090922 | 63090929 | 63090922 | ID=Msa0614170-mRNA-1:cds;Parent=Msa0614170-mRNA-1 |
| chr4_2 | CDS | 63091125 | 63091449 | 63091125 | ID=Msa0614170-mRNA-1:cds;Parent=Msa0614170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
