Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0617180 | XP_013446497.1 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 7.43e-41 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0617180 | sp|O22860|RL38_ARATH | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.31e-40 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0617180 | A0A2Z6P5F7 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 3.55e-41 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0617180 | Msa0656190 | 0.921199 | 4.858245e-88 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0617180 | MtrunA17_Chr8g0376751 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 6.83e-45 | 138 |
| Msa0617180 | MtrunA17_Chr4g0033761 | 94.203 | 69 | 4 | 0 | 1 | 69 | 1 | 69 | 3.74e-42 | 131 |
| Msa0617180 | MtrunA17_Chr2g0285451 | 89.855 | 69 | 7 | 0 | 1 | 69 | 1 | 69 | 6.17e-40 | 125 |
| Msa0617180 | MtrunA17_Chr7g0216191 | 85.507 | 69 | 10 | 0 | 1 | 69 | 1 | 69 | 3.44e-35 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0617180 | AT2G43460.1 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.33e-41 | 130 |
| Msa0617180 | AT3G59540.1 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 1.33e-41 | 130 |
Find 23 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGATGTTATGGTTAATA+TGG | 0.267232 | 4_2:-67580968 | Msa0617180:three_prime_UTR |
| ATTTAGCTTAATTGATGTTA+TGG | 0.292742 | 4_2:-67580977 | Msa0617180:three_prime_UTR |
| GTTTCTTGTGGCTGCAAATA+GGG | 0.313902 | 4_2:+67581495 | None:intergenic |
| CTATGAGATTTGAAGATGTT+TGG | 0.339052 | 4_2:-67581016 | Msa0617180:three_prime_UTR |
| AATTCCAAGTCACAAAATCT+AGG | 0.350296 | 4_2:+67581083 | None:intergenic |
| GGTTTCTTGTGGCTGCAAAT+AGG | 0.362063 | 4_2:+67581494 | None:intergenic |
| GGGTTTCTTCCTCGCCTCTT+TGG | 0.396940 | 4_2:+67581515 | None:intergenic |
| GAAGAAAATCCTTGATTTCG+TGG | 0.412070 | 4_2:+67581342 | None:intergenic |
| TCTTCTGACAGCGCGTAGAA+AGG | 0.474822 | 4_2:-67581324 | Msa0617180:CDS |
| ACCATTGATGTGGTTTCTTG+TGG | 0.506319 | 4_2:+67581483 | None:intergenic |
| AGTGAAAATCAAGAGAAGTA+AGG | 0.506775 | 4_2:-67581291 | Msa0617180:CDS |
| GATATTTATTGCTTATATGA+TGG | 0.507627 | 4_2:-67580908 | Msa0617180:three_prime_UTR |
| TAAGGATGTGGTGAAGTTCA+AGG | 0.511754 | 4_2:-67581273 | Msa0617180:CDS |
| ATTCCAAGTCACAAAATCTA+GGG | 0.522380 | 4_2:+67581084 | None:intergenic |
| TTCTGAAGCTTATTCCAAAG+AGG | 0.534150 | 4_2:-67581529 | None:intergenic |
| GAAGCAGATCCACGAAATCA+AGG | 0.537316 | 4_2:-67581351 | Msa0617180:CDS |
| GCCACAAGAAACCACATCAA+TGG | 0.560468 | 4_2:-67581484 | Msa0617180:intron |
| TTGAAGCAATCTCTTCCACC+TGG | 0.570275 | 4_2:-67581191 | Msa0617180:CDS |
| AATCAAGAGAAGTAAGGATG+TGG | 0.579591 | 4_2:-67581285 | Msa0617180:CDS |
| AGTGGGAAGTACCATTGATG+TGG | 0.614301 | 4_2:+67581473 | None:intergenic |
| AGATCTTGAACAGTCAAACC+AGG | 0.648645 | 4_2:+67581173 | None:intergenic |
| AAGCTTATTCCAAAGAGGCG+AGG | 0.669151 | 4_2:-67581524 | Msa0617180:five_prime_UTR |
| TCTTGAACAGTCAAACCAGG+TGG | 0.706952 | 4_2:+67581176 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAGTATTTTTTGTGTTAATA+TGG | - | chr4_2:67581423-67581442 | Msa0617180:intron | 15.0% |
| !!! | TTGGAATTATGTTTTGTAAT+TGG | - | chr4_2:67581300-67581319 | Msa0617180:CDS | 20.0% |
| !!! | ATTTAGCTTAATTGATGTTA+TGG | - | chr4_2:67581391-67581410 | Msa0617180:intron | 20.0% |
| !! | AATTGATGTTATGGTTAATA+TGG | - | chr4_2:67581400-67581419 | Msa0617180:intron | 20.0% |
| !! | GATATTTATTGCTTATATGA+TGG | - | chr4_2:67581460-67581479 | Msa0617180:intron | 20.0% |
| !!! | ATGTATTGTGTTGTTTTTTC+AGG | - | chr4_2:67580993-67581012 | Msa0617180:three_prime_UTR | 25.0% |
| TCCAATTAACGAAAGAAAGT+GGG | + | chr4_2:67580915-67580934 | None:intergenic | 30.0% | |
| AGTGAAAATCAAGAGAAGTA+AGG | - | chr4_2:67581077-67581096 | Msa0617180:three_prime_UTR | 30.0% | |
| ATTCCAAGTCACAAAATCTA+GGG | + | chr4_2:67581287-67581306 | None:intergenic | 30.0% | |
| AATTCCAAGTCACAAAATCT+AGG | + | chr4_2:67581288-67581307 | None:intergenic | 30.0% | |
| CTATGAGATTTGAAGATGTT+TGG | - | chr4_2:67581352-67581371 | Msa0617180:CDS | 30.0% | |
| TCCCACTTTCTTTCGTTAAT+TGG | - | chr4_2:67580911-67580930 | Msa0617180:three_prime_UTR | 35.0% | |
| CTCCAATTAACGAAAGAAAG+TGG | + | chr4_2:67580916-67580935 | None:intergenic | 35.0% | |
| GAAGAAAATCCTTGATTTCG+TGG | + | chr4_2:67581029-67581048 | None:intergenic | 35.0% | |
| AATCAAGAGAAGTAAGGATG+TGG | - | chr4_2:67581083-67581102 | Msa0617180:three_prime_UTR | 35.0% | |
| ! | AAACCCTAGATTTTGTGACT+TGG | - | chr4_2:67581281-67581300 | Msa0617180:CDS | 35.0% |
| GTTTCTTGTGGCTGCAAATA+GGG | + | chr4_2:67580876-67580895 | None:intergenic | 40.0% | |
| ACCATTGATGTGGTTTCTTG+TGG | + | chr4_2:67580888-67580907 | None:intergenic | 40.0% | |
| TAAGGATGTGGTGAAGTTCA+AGG | - | chr4_2:67581095-67581114 | Msa0617180:three_prime_UTR | 40.0% | |
| AGATCTTGAACAGTCAAACC+AGG | + | chr4_2:67581198-67581217 | None:intergenic | 40.0% | |
| AAGCTTATTCCAAAGAGGCG+AGG | - | chr4_2:67580844-67580863 | Msa0617180:three_prime_UTR | 45.0% | |
| GGTTTCTTGTGGCTGCAAAT+AGG | + | chr4_2:67580877-67580896 | None:intergenic | 45.0% | |
| GCCACAAGAAACCACATCAA+TGG | - | chr4_2:67580884-67580903 | Msa0617180:three_prime_UTR | 45.0% | |
| !! | AGTGGGAAGTACCATTGATG+TGG | + | chr4_2:67580898-67580917 | None:intergenic | 45.0% |
| GAAGCAGATCCACGAAATCA+AGG | - | chr4_2:67581017-67581036 | Msa0617180:three_prime_UTR | 45.0% | |
| TTGAAGCAATCTCTTCCACC+TGG | - | chr4_2:67581177-67581196 | Msa0617180:CDS | 45.0% | |
| TCTTGAACAGTCAAACCAGG+TGG | + | chr4_2:67581195-67581214 | None:intergenic | 45.0% | |
| TCTTCTGACAGCGCGTAGAA+AGG | - | chr4_2:67581044-67581063 | Msa0617180:three_prime_UTR | 50.0% | |
| GGGTTTCTTCCTCGCCTCTT+TGG | + | chr4_2:67580856-67580875 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 67580843 | 67581547 | 67580843 | ID=Msa0617180;Name=Msa0617180 |
| chr4_2 | mRNA | 67580843 | 67581547 | 67580843 | ID=Msa0617180-mRNA-1;Parent=Msa0617180;Name=Msa0617180-mRNA-1;_AED=0.00;_eAED=0.00;_QI=60|1|1|1|1|1|2|326|69 |
| chr4_2 | exon | 67580843 | 67581375 | 67580843 | ID=Msa0617180-mRNA-1:exon:19320;Parent=Msa0617180-mRNA-1 |
| chr4_2 | exon | 67581485 | 67581547 | 67581485 | ID=Msa0617180-mRNA-1:exon:19319;Parent=Msa0617180-mRNA-1 |
| chr4_2 | five_prime_UTR | 67581488 | 67581547 | 67581488 | ID=Msa0617180-mRNA-1:five_prime_utr;Parent=Msa0617180-mRNA-1 |
| chr4_2 | CDS | 67581485 | 67581487 | 67581485 | ID=Msa0617180-mRNA-1:cds;Parent=Msa0617180-mRNA-1 |
| chr4_2 | CDS | 67581169 | 67581375 | 67581169 | ID=Msa0617180-mRNA-1:cds;Parent=Msa0617180-mRNA-1 |
| chr4_2 | three_prime_UTR | 67580843 | 67581168 | 67580843 | ID=Msa0617180-mRNA-1:three_prime_utr;Parent=Msa0617180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
