AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0635730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0635730 MtrunA17_Chr4g0018191 93.413 167 11 0 1 167 30 196 9.21e-105 298
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0635730 AT5G51545.1 40.860 186 82 4 1 163 1 181 1.39e-37 127

Find 56 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAAATTGTCCCACTCTTC+TGG 0.220374 4_3:+25049165 None:intergenic
CAATCCCATTTGTAATTAAC+TGG 0.242118 4_3:-25048820 Msa0635730:three_prime_UTR
AACTGGATTACGCCGAATTA+TGG 0.243755 4_3:+25051585 None:intergenic
TCACCTGTCATCTTCGATTC+TGG 0.267437 4_3:+25048936 None:intergenic
TGATGATTGCAATCCTATTC+TGG 0.271384 4_3:-25048969 Msa0635730:three_prime_UTR
GGTATTGCTCTTGCTGCTTC+AGG 0.275480 4_3:-25051442 Msa0635730:intron
GAAGAGAGTGCTGTTGAAAA+AGG 0.279029 4_3:-25051511 Msa0635730:CDS
AACAAAACTGGTGTGGGTTT+TGG 0.286345 4_3:-25051664 Msa0635730:CDS
ACAGTATTATTGTTTCTTGC+TGG 0.296795 4_3:-25049111 Msa0635730:CDS
CTTGGCCATATGATGCAATA+TGG 0.300103 4_3:+25048867 None:intergenic
CTTGCTGGAGCAGTAGCATT+TGG 0.343726 4_3:-25049096 Msa0635730:CDS
GAGAAAGAGCTTCCATAATT+CGG 0.373166 4_3:-25051597 Msa0635730:CDS
TCGGACTTGATTGTGAAGAT+AGG 0.376155 4_3:+25051718 None:intergenic
ATTGTTGCTTGGCTTGGTTT+TGG 0.377960 4_3:-25051484 Msa0635730:CDS
GCAATATGGGGTTGCTGCTT+AGG 0.394933 4_3:+25048881 None:intergenic
GGTTCGGTTATTCTTGTTGA+GGG 0.408372 4_3:-25051463 Msa0635730:CDS
GTAATCCAGTTAATTACAAA+TGG 0.412509 4_3:+25048815 None:intergenic
TTTCATAAACCTTAGTTACT+TGG 0.416349 4_3:+25048849 None:intergenic
GATTCAGTTCCCAACAAAAC+TGG 0.426754 4_3:-25051676 Msa0635730:CDS
CAACCCCATATTGCATCATA+TGG 0.433321 4_3:-25048872 Msa0635730:three_prime_UTR
AGAGAATGAGTGATGTAGTT+TGG 0.434413 4_3:+25051741 None:intergenic
TGGCAAATTGAAGCCAGAAT+AGG 0.436939 4_3:+25048956 None:intergenic
CAATAATACTGTAGGGGTAA+AGG 0.457025 4_3:+25049122 None:intergenic
ATCATATGGCCAAGTAACTA+AGG 0.459857 4_3:-25048858 Msa0635730:three_prime_UTR
TCTTGCAAGGAAGTTTCAAT+TGG 0.461652 4_3:+25048717 None:intergenic
GAAGATTGATCAATTTATGT+TGG 0.462950 4_3:-25048906 Msa0635730:three_prime_UTR
TTGGCCATATGATGCAATAT+GGG 0.475258 4_3:+25048868 None:intergenic
CGAATGGCAACAACACTAAA+CGG 0.483957 4_3:-25051636 Msa0635730:CDS
GCAAGAAACAATAATACTGT+AGG 0.502406 4_3:+25049114 None:intergenic
AACACTAAACGGAAACAGAA+AGG 0.503067 4_3:-25051625 Msa0635730:CDS
TAATCCAGTTAATTACAAAT+GGG 0.503901 4_3:+25048816 None:intergenic
CAAGAAACAATAATACTGTA+GGG 0.504164 4_3:+25049115 None:intergenic
TGTTAGAAATCTCTAATGTC+CGG 0.506879 4_3:-25049010 Msa0635730:three_prime_UTR
TGGTTCGGTTATTCTTGTTG+AGG 0.511249 4_3:-25051464 Msa0635730:CDS
AATTGTTCCGAAACGAAAGC+GGG 0.515007 4_3:+25051556 None:intergenic
TTAGTGTTGTTGCCATTCGA+CGG 0.524904 4_3:+25051640 None:intergenic
ACACTAAACGGAAACAGAAA+GGG 0.536364 4_3:-25051624 Msa0635730:CDS
CAATTGTTCCGAAACGAAAG+CGG 0.548432 4_3:+25051555 None:intergenic
TTGGAACCTGAATTCTTGCA+AGG 0.561437 4_3:+25048704 None:intergenic
TTGCCAGAATCGAAGATGAC+AGG 0.563968 4_3:-25048939 Msa0635730:three_prime_UTR
AATGAGTGATGTAGTTTGGT+GGG 0.564862 4_3:+25051745 None:intergenic
GTGATGTAGTTTGGTGGGGA+AGG 0.567049 4_3:+25051750 None:intergenic
GTTCCCAACAAAACTGGTGT+GGG 0.575858 4_3:-25051670 Msa0635730:CDS
AGTTCCCAACAAAACTGGTG+TGG 0.579102 4_3:-25051671 Msa0635730:CDS
ACTGAATCGGACGGTTCAGA+AGG 0.586590 4_3:+25051691 None:intergenic
AATACTGTAGGGGTAAAGGA+TGG 0.613012 4_3:+25049126 None:intergenic
TGGAGCTTAGTTTCACCACA+TGG 0.617051 4_3:+25051818 None:intergenic
GAATGAGTGATGTAGTTTGG+TGG 0.628646 4_3:+25051744 None:intergenic
AAGTAACTAAGGTTTATGAA+AGG 0.629581 4_3:-25048847 Msa0635730:three_prime_UTR
ATGGATAGAGATATTTAACA+AGG 0.638121 4_3:+25049145 None:intergenic
TAAGCAGAATGAGAAACTCA+CGG 0.646278 4_3:-25049061 Msa0635730:CDS
AAGAAACAATAATACTGTAG+GGG 0.647405 4_3:+25049116 None:intergenic
ATGAGTGATGTAGTTTGGTG+GGG 0.673147 4_3:+25051746 None:intergenic
TTGTTGGGAACTGAATCGGA+CGG 0.702263 4_3:+25051682 None:intergenic
TGGCCATATGATGCAATATG+GGG 0.716203 4_3:+25048869 None:intergenic
TGGAGCAGTAGCATTTGGAG+TGG 0.720186 4_3:-25049091 Msa0635730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTATTAAAAATAAAAAGA+AGG + chr4_3:25049163-25049182 None:intergenic 10.0%
!!! ATTAAGTTTATTTAATTTTG+TGG - chr4_3:25049199-25049218 Msa0635730:intron 10.0%
!! AATTAAGATAAATAATGATA+GGG - chr4_3:25049582-25049601 Msa0635730:intron 10.0%
!! TTAATACTATTTATTATTCA+AGG - chr4_3:25051083-25051102 Msa0635730:intron 10.0%
!!! TTTTTATTAAATTATGTACT+AGG - chr4_3:25051783-25051802 Msa0635730:exon 10.0%
!!! TTATTTTCAATTAAGGTTAT+AGG - chr4_3:25049554-25049573 Msa0635730:intron 15.0%
!! GAATTAAGATAAATAATGAT+AGG - chr4_3:25049581-25049600 Msa0635730:intron 15.0%
!!! TATTTTGTCATTTTTTACAT+AGG + chr4_3:25050314-25050333 None:intergenic 15.0%
!! ATGTTCATTTAAAAAATTTG+AGG - chr4_3:25051128-25051147 Msa0635730:intron 15.0%
!! AGAAATGAAAAACAATAATT+TGG + chr4_3:25051155-25051174 None:intergenic 15.0%
!!! CTTAATGAGTTTTCAAAATT+AGG + chr4_3:25049185-25049204 None:intergenic 20.0%
!! TTGAAGTAGTTTATCAAAAA+TGG - chr4_3:25049629-25049648 Msa0635730:intron 20.0%
!! ATAACTATAAGTTCAAAAAC+AGG - chr4_3:25049726-25049745 Msa0635730:intron 20.0%
!! CACTTATTATTATAAGCTTA+TGG + chr4_3:25049817-25049836 None:intergenic 20.0%
!! GATCTTTACTCTAAATTTAA+AGG + chr4_3:25050175-25050194 None:intergenic 20.0%
!!! ATTAGAATTTAGGATCTAAA+TGG - chr4_3:25050712-25050731 Msa0635730:intron 20.0%
!!! TTAGAATTTAGGATCTAAAT+GGG - chr4_3:25050713-25050732 Msa0635730:intron 20.0%
!! TTTGTTGAAATTAATGATTG+AGG - chr4_3:25050758-25050777 Msa0635730:intron 20.0%
!! TAATCCAGTTAATTACAAAT+GGG + chr4_3:25051759-25051778 None:intergenic 20.0%
!!! AAAAAGGTTTTATTGTTGCT+TGG - chr4_3:25049077-25049096 Msa0635730:CDS 25.0%
! TTGCAGTTAGAGTAAATTAA+GGG - chr4_3:25049239-25049258 Msa0635730:intron 25.0%
!! TTTTCAATTAAGGTTATAGG+AGG - chr4_3:25049557-25049576 Msa0635730:intron 25.0%
! TTTCAATTAAGGTTATAGGA+GGG - chr4_3:25049558-25049577 Msa0635730:intron 25.0%
!!! CTAATAAGCTGTTTTCTTAT+GGG + chr4_3:25049766-25049785 None:intergenic 25.0%
!!! TCTAATAAGCTGTTTTCTTA+TGG + chr4_3:25049767-25049786 None:intergenic 25.0%
! ATAAGAGATTATGCTATAAG+CGG - chr4_3:25049873-25049892 Msa0635730:intron 25.0%
!! GGAAGTATATATGTTAGAAT+AGG - chr4_3:25050058-25050077 Msa0635730:intron 25.0%
!! TAGTGTTGAAAGTTTGAATA+TGG - chr4_3:25050375-25050394 Msa0635730:intron 25.0%
!! TGTATTTGAATGCTATGTTT+TGG - chr4_3:25050652-25050671 Msa0635730:intron 25.0%
!! GTATTTGAATGCTATGTTTT+GGG - chr4_3:25050653-25050672 Msa0635730:intron 25.0%
!!! GGATTTATTTTTATTCTCTC+TGG - chr4_3:25050892-25050911 Msa0635730:intron 25.0%
! AGAGACTATCAATTACAATA+TGG - chr4_3:25050959-25050978 Msa0635730:intron 25.0%
! GAGACTATCAATTACAATAT+GGG - chr4_3:25050960-25050979 Msa0635730:intron 25.0%
!! ACATTGATACTTTCATTTTC+AGG + chr4_3:25051035-25051054 None:intergenic 25.0%
!!! TAACTCGTTCTTTAGAATAA+AGG + chr4_3:25051230-25051249 None:intergenic 25.0%
! GTAAAATGACACTAATTCTT+AGG + chr4_3:25051341-25051360 None:intergenic 25.0%
! ATGGATAGAGATATTTAACA+AGG + chr4_3:25051430-25051449 None:intergenic 25.0%
! AAGAAACAATAATACTGTAG+GGG + chr4_3:25051459-25051478 None:intergenic 25.0%
! CAAGAAACAATAATACTGTA+GGG + chr4_3:25051460-25051479 None:intergenic 25.0%
! GAAGATTGATCAATTTATGT+TGG - chr4_3:25051666-25051685 Msa0635730:CDS 25.0%
! TTTCATAAACCTTAGTTACT+TGG + chr4_3:25051726-25051745 None:intergenic 25.0%
! AAGTAACTAAGGTTTATGAA+AGG - chr4_3:25051725-25051744 Msa0635730:CDS 25.0%
! GTAATCCAGTTAATTACAAA+TGG + chr4_3:25051760-25051779 None:intergenic 25.0%
ATCAGAAAAAAGAAACCATG+TGG - chr4_3:25048739-25048758 Msa0635730:three_prime_UTR 30.0%
!!! CATTTTTGTTTTGTTGGAGT+TGG + chr4_3:25048777-25048796 None:intergenic 30.0%
CAACTCCAACAAAACAAAAA+TGG - chr4_3:25048775-25048794 Msa0635730:three_prime_UTR 30.0%
GTTGCAGTTAGAGTAAATTA+AGG - chr4_3:25049238-25049257 Msa0635730:intron 30.0%
TTTGAGCTAATCTTGTAAGA+CGG - chr4_3:25049280-25049299 Msa0635730:intron 30.0%
AAACAAAACTAAACCCACTT+GGG + chr4_3:25049459-25049478 None:intergenic 30.0%
TTCAATTAAGGTTATAGGAG+GGG - chr4_3:25049559-25049578 Msa0635730:intron 30.0%
! TGTTTTCTTATGGGTCTTTT+TGG + chr4_3:25049757-25049776 None:intergenic 30.0%
! TCGTACTTCTCTTTCTTTTA+GGG + chr4_3:25049948-25049967 None:intergenic 30.0%
! TTCGTACTTCTCTTTCTTTT+AGG + chr4_3:25049949-25049968 None:intergenic 30.0%
GTTCTATTGAAGTTTAGAGA+TGG - chr4_3:25050342-25050361 Msa0635730:intron 30.0%
TTTGAATATGGCTGAAGAAT+TGG - chr4_3:25050387-25050406 Msa0635730:intron 30.0%
GTCCTTTCTATTACCAATTT+GGG + chr4_3:25050523-25050542 None:intergenic 30.0%
ACAGAATTTACCAGATTATC+CGG + chr4_3:25050546-25050565 None:intergenic 30.0%
!! TTGTGTTAATGCTCTGAAAA+GGG + chr4_3:25050686-25050705 None:intergenic 30.0%
ACTATCAATTACAATATGGG+AGG - chr4_3:25050963-25050982 Msa0635730:intron 30.0%
TGGGAGGAAAAATAAAGTAT+CGG - chr4_3:25050979-25050998 Msa0635730:intron 30.0%
CTGATTAAGATACAATAGCT+TGG + chr4_3:25051291-25051310 None:intergenic 30.0%
! CTTAATCAGTTAGTTGACTT+AGG - chr4_3:25051302-25051321 Msa0635730:intron 30.0%
GCAAGAAACAATAATACTGT+AGG + chr4_3:25051461-25051480 None:intergenic 30.0%
! ACAGTATTATTGTTTCTTGC+TGG - chr4_3:25051461-25051480 Msa0635730:CDS 30.0%
TGTTAGAAATCTCTAATGTC+CGG - chr4_3:25051562-25051581 Msa0635730:CDS 30.0%
!!! ATCACTCATAGTACTTTTTC+CGG + chr4_3:25051584-25051603 None:intergenic 30.0%
CAATCCCATTTGTAATTAAC+TGG - chr4_3:25051752-25051771 Msa0635730:CDS 30.0%
!!! TAGCGCCATTTTTGTTTTGT+TGG + chr4_3:25048783-25048802 None:intergenic 35.0%
AATGAGTGATGTAGTTTGGT+GGG + chr4_3:25048830-25048849 None:intergenic 35.0%
AGAGAATGAGTGATGTAGTT+TGG + chr4_3:25048834-25048853 None:intergenic 35.0%
!!! AGTTTTGTTGGGAACTGAAT+CGG + chr4_3:25048897-25048916 None:intergenic 35.0%
AACACTAAACGGAAACAGAA+AGG - chr4_3:25048947-25048966 Msa0635730:three_prime_UTR 35.0%
ACACTAAACGGAAACAGAAA+GGG - chr4_3:25048948-25048967 Msa0635730:three_prime_UTR 35.0%
GAGAAAGAGCTTCCATAATT+CGG - chr4_3:25048975-25048994 Msa0635730:three_prime_UTR 35.0%
GAGTAAATTAAGGGTCTGTT+TGG - chr4_3:25049248-25049267 Msa0635730:intron 35.0%
AATTAAGGGTCTGTTTGGAT+TGG - chr4_3:25049253-25049272 Msa0635730:intron 35.0%
! TCGGTTATAAGCTAGCTTAT+CGG + chr4_3:25049352-25049371 None:intergenic 35.0%
! AGCTAGCTTATAACCGATAA+CGG - chr4_3:25049355-25049374 Msa0635730:intron 35.0%
!!! AGCTAGCTTTTATCCGTTAT+CGG + chr4_3:25049371-25049390 None:intergenic 35.0%
CAAACAAAACTAAACCCACT+TGG + chr4_3:25049460-25049479 None:intergenic 35.0%
!!! CAAGTGGGTTTAGTTTTGTT+TGG - chr4_3:25049458-25049477 Msa0635730:intron 35.0%
ATAAGCTTGTGAGAAAGTGA+CGG - chr4_3:25049654-25049673 Msa0635730:intron 35.0%
GTACGAATTTGCAATCAACA+TGG - chr4_3:25049962-25049981 Msa0635730:intron 35.0%
GATTTGATTAGCAGTGTGTA+TGG - chr4_3:25050037-25050056 Msa0635730:intron 35.0%
!! GAAACTAGTCTCTGATTTTC+TGG - chr4_3:25050114-25050133 Msa0635730:intron 35.0%
CGTCACTTCTTAAAGAGTTT+AGG - chr4_3:25050269-25050288 Msa0635730:intron 35.0%
GGTCCTTTCTATTACCAATT+TGG + chr4_3:25050524-25050543 None:intergenic 35.0%
! AATTGGTAATAGAAAGGACC+CGG - chr4_3:25050524-25050543 Msa0635730:intron 35.0%
CAGAATTTACCAGATTATCC+GGG + chr4_3:25050545-25050564 None:intergenic 35.0%
!!! TGAATGCTATGTTTTGGGTT+AGG - chr4_3:25050658-25050677 Msa0635730:intron 35.0%
!! TGTGTTAATGCTCTGAAAAG+GGG + chr4_3:25050685-25050704 None:intergenic 35.0%
!! GTTGTGTTAATGCTCTGAAA+AGG + chr4_3:25050687-25050706 None:intergenic 35.0%
ACAACCAGGGATTAGAATTT+AGG - chr4_3:25050702-25050721 Msa0635730:intron 35.0%
AGATCCTAAATTCTAATCCC+TGG + chr4_3:25050709-25050728 None:intergenic 35.0%
AGGATCTAAATGGGAAAAGT+AGG - chr4_3:25050722-25050741 Msa0635730:intron 35.0%
GGATCTAAATGGGAAAAGTA+GGG - chr4_3:25050723-25050742 Msa0635730:intron 35.0%
CTAACTCTTCTTCTTCTTCA+TGG + chr4_3:25050831-25050850 None:intergenic 35.0%
ACTCTCGATATGTTGTCAAT+AGG + chr4_3:25050866-25050885 None:intergenic 35.0%
GACACTAATTCTTAGGTTCT+AGG + chr4_3:25051334-25051353 None:intergenic 35.0%
!! GTCATTTTACATCGATACTG+TGG - chr4_3:25051351-25051370 Msa0635730:intron 35.0%
CGATACTGTGGTTAATACTT+TGG - chr4_3:25051363-25051382 Msa0635730:intron 35.0%
! ATACTTTGGTGTGTTATTGC+AGG - chr4_3:25051377-25051396 Msa0635730:intron 35.0%
CAATAATACTGTAGGGGTAA+AGG + chr4_3:25051453-25051472 None:intergenic 35.0%
TAAGCAGAATGAGAAACTCA+CGG - chr4_3:25051511-25051530 Msa0635730:CDS 35.0%
TGATGATTGCAATCCTATTC+TGG - chr4_3:25051603-25051622 Msa0635730:CDS 35.0%
TTGGCCATATGATGCAATAT+GGG + chr4_3:25051707-25051726 None:intergenic 35.0%
ATCATATGGCCAAGTAACTA+AGG - chr4_3:25051714-25051733 Msa0635730:CDS 35.0%
TCTTGCAAGGAAGTTTCAAT+TGG + chr4_3:25051858-25051877 None:intergenic 35.0%
! ATGAGTGATGTAGTTTGGTG+GGG + chr4_3:25048829-25048848 None:intergenic 40.0%
GAATGAGTGATGTAGTTTGG+TGG + chr4_3:25048831-25048850 None:intergenic 40.0%
TCGGACTTGATTGTGAAGAT+AGG + chr4_3:25048857-25048876 None:intergenic 40.0%
GATTCAGTTCCCAACAAAAC+TGG - chr4_3:25048896-25048915 Msa0635730:three_prime_UTR 40.0%
!!! AAACCCACACCAGTTTTGTT+GGG + chr4_3:25048908-25048927 None:intergenic 40.0%
!! AAAACCCACACCAGTTTTGT+TGG + chr4_3:25048909-25048928 None:intergenic 40.0%
! AACAAAACTGGTGTGGGTTT+TGG - chr4_3:25048908-25048927 Msa0635730:three_prime_UTR 40.0%
! TTAGTGTTGTTGCCATTCGA+CGG + chr4_3:25048935-25048954 None:intergenic 40.0%
CGAATGGCAACAACACTAAA+CGG - chr4_3:25048936-25048955 Msa0635730:three_prime_UTR 40.0%
AACTGGATTACGCCGAATTA+TGG + chr4_3:25048990-25049009 None:intergenic 40.0%
TGAAAAACCCGCTTTCGTTT+CGG - chr4_3:25049009-25049028 Msa0635730:three_prime_UTR 40.0%
AATTGTTCCGAAACGAAAGC+GGG + chr4_3:25049019-25049038 None:intergenic 40.0%
CAATTGTTCCGAAACGAAAG+CGG + chr4_3:25049020-25049039 None:intergenic 40.0%
!! GAAGAGAGTGCTGTTGAAAA+AGG - chr4_3:25049061-25049080 Msa0635730:CDS 40.0%
!!! GGTTTTATTGTTGCTTGGCT+TGG - chr4_3:25049082-25049101 Msa0635730:CDS 40.0%
!!! ATTGTTGCTTGGCTTGGTTT+TGG - chr4_3:25049088-25049107 Msa0635730:CDS 40.0%
!! TGGTTCGGTTATTCTTGTTG+AGG - chr4_3:25049108-25049127 Msa0635730:CDS 40.0%
! GGTTCGGTTATTCTTGTTGA+GGG - chr4_3:25049109-25049128 Msa0635730:CDS 40.0%
GCTAATCTTGTAAGACGGTT+TGG - chr4_3:25049285-25049304 Msa0635730:intron 40.0%
GTCTCTTAAGCATGTGGTTT+GGG - chr4_3:25050015-25050034 Msa0635730:intron 40.0%
! GAATAGGTCAGCCTCTTTTA+TGG - chr4_3:25050074-25050093 Msa0635730:intron 40.0%
GGTACGGAAATCCATAAAAG+AGG + chr4_3:25050088-25050107 None:intergenic 40.0%
!! CTAGTCTCTGATTTTCTGGT+TGG - chr4_3:25050118-25050137 Msa0635730:intron 40.0%
!! TTTCTGGTTGGAGGATAACT+TGG - chr4_3:25050130-25050149 Msa0635730:intron 40.0%
! TTCTGGTTGGAGGATAACTT+GGG - chr4_3:25050131-25050150 Msa0635730:intron 40.0%
GTGTCATGTTTGTGCATCAT+AGG + chr4_3:25050202-25050221 None:intergenic 40.0%
GTGACAAAATAACCCAAGTC+CGG - chr4_3:25050452-25050471 Msa0635730:intron 40.0%
ATTACCAATTTGGGCCAACA+GGG + chr4_3:25050514-25050533 None:intergenic 40.0%
TATTACCAATTTGGGCCAAC+AGG + chr4_3:25050515-25050534 None:intergenic 40.0%
! GGCCCAAATTGGTAATAGAA+AGG - chr4_3:25050518-25050537 Msa0635730:intron 40.0%
!! GTGTTAATGCTCTGAAAAGG+GGG + chr4_3:25050684-25050703 None:intergenic 40.0%
! TTCAGAGCATTAACACAACC+AGG - chr4_3:25050688-25050707 Msa0635730:intron 40.0%
! TCAGAGCATTAACACAACCA+GGG - chr4_3:25050689-25050708 Msa0635730:intron 40.0%
! CAACATATCGAGAGTGTGTA+AGG - chr4_3:25050871-25050890 Msa0635730:intron 40.0%
ACTATGACCCAAACCTAAGA+TGG - chr4_3:25051262-25051281 Msa0635730:intron 40.0%
TTGGAGACCATCTTAGGTTT+GGG + chr4_3:25051272-25051291 None:intergenic 40.0%
AATAGCTTGGAGACCATCTT+AGG + chr4_3:25051278-25051297 None:intergenic 40.0%
!!! AGGCTTTTTTCCAGAAGAGT+GGG - chr4_3:25051397-25051416 Msa0635730:intron 40.0%
AATACTGTAGGGGTAAAGGA+TGG + chr4_3:25051449-25051468 None:intergenic 40.0%
TGGCAAATTGAAGCCAGAAT+AGG + chr4_3:25051619-25051638 None:intergenic 40.0%
CAACCCCATATTGCATCATA+TGG - chr4_3:25051700-25051719 Msa0635730:CDS 40.0%
TGGCCATATGATGCAATATG+GGG + chr4_3:25051706-25051725 None:intergenic 40.0%
CTTGGCCATATGATGCAATA+TGG + chr4_3:25051708-25051727 None:intergenic 40.0%
TGGAGCTTAGTTTCACCACA+TGG + chr4_3:25048757-25048776 None:intergenic 45.0%
! GGACGGTTCAGAAGGTTTTT+CGG + chr4_3:25048876-25048895 None:intergenic 45.0%
TTGTTGGGAACTGAATCGGA+CGG + chr4_3:25048893-25048912 None:intergenic 45.0%
AGTTCCCAACAAAACTGGTG+TGG - chr4_3:25048901-25048920 Msa0635730:three_prime_UTR 45.0%
GTTCCCAACAAAACTGGTGT+GGG - chr4_3:25048902-25048921 Msa0635730:three_prime_UTR 45.0%
!! ACGAAAGCGGGTTTTTCAAC+TGG + chr4_3:25049007-25049026 None:intergenic 45.0%
!!! TGCTTGGCTTGGTTTTGGTT+CGG - chr4_3:25049093-25049112 Msa0635730:CDS 45.0%
AGCTAGCTGATGAGACCTAT+AGG - chr4_3:25049383-25049402 Msa0635730:intron 45.0%
TAGCTGATGAGACCTATAGG+AGG - chr4_3:25049386-25049405 Msa0635730:intron 45.0%
TTGGAGTATGCTCCTCCTAT+AGG + chr4_3:25049401-25049420 None:intergenic 45.0%
! TATAGGAGGAGCATACTCCA+AGG - chr4_3:25049400-25049419 Msa0635730:intron 45.0%
GCAATCAACATGGTGTGACA+TGG - chr4_3:25049972-25049991 Msa0635730:intron 45.0%
!! GGTTGACTTGAGAGATAACC+AGG - chr4_3:25049993-25050012 Msa0635730:intron 45.0%
AACCAGGTCTCTTAAGCATG+TGG - chr4_3:25050009-25050028 Msa0635730:intron 45.0%
AACCACATGCTTAAGAGACC+TGG + chr4_3:25050014-25050033 None:intergenic 45.0%
GGTCTCTTAAGCATGTGGTT+TGG - chr4_3:25050014-25050033 Msa0635730:intron 45.0%
!! GACTAGTTTCTCTCGAGGTA+CGG + chr4_3:25050104-25050123 None:intergenic 45.0%
!! TCAGAGACTAGTTTCTCTCG+AGG + chr4_3:25050109-25050128 None:intergenic 45.0%
!!! GTCTCTGATTTTCTGGTTGG+AGG - chr4_3:25050121-25050140 Msa0635730:intron 45.0%
TAGAAAGGACCCGGATAATC+TGG - chr4_3:25050533-25050552 Msa0635730:intron 45.0%
CTTGGAGACCATCTTAGGTT+TGG + chr4_3:25051273-25051292 None:intergenic 45.0%
!!! CAGGCTTTTTTCCAGAAGAG+TGG - chr4_3:25051396-25051415 Msa0635730:intron 45.0%
AGGAAATTGTCCCACTCTTC+TGG + chr4_3:25051410-25051429 None:intergenic 45.0%
TTGCCAGAATCGAAGATGAC+AGG - chr4_3:25051633-25051652 Msa0635730:CDS 45.0%
TCACCTGTCATCTTCGATTC+TGG + chr4_3:25051639-25051658 None:intergenic 45.0%
!!! TTTTATTTTATTTTCAATTA+AGG - chr4_3:25049547-25049566 Msa0635730:intron 5.0%
! GTGATGTAGTTTGGTGGGGA+AGG + chr4_3:25048825-25048844 None:intergenic 50.0%
ACTGAATCGGACGGTTCAGA+AGG + chr4_3:25048884-25048903 None:intergenic 50.0%
! GGTATTGCTCTTGCTGCTTC+AGG - chr4_3:25049130-25049149 Msa0635730:CDS 50.0%
GCTTATCGGTCATGCGCTAT+AGG + chr4_3:25049338-25049357 None:intergenic 50.0%
!! TAAACCCACTTGGGTTAGCC+AGG + chr4_3:25049450-25049469 None:intergenic 50.0%
CTTGCTGGAGCAGTAGCATT+TGG - chr4_3:25051476-25051495 Msa0635730:CDS 50.0%
TGGAGCAGTAGCATTTGGAG+TGG - chr4_3:25051481-25051500 Msa0635730:CDS 50.0%
! GCAATATGGGGTTGCTGCTT+AGG + chr4_3:25051694-25051713 None:intergenic 50.0%
!! GTGGGTTTTGGTCCGTCGAA+TGG - chr4_3:25048920-25048939 Msa0635730:three_prime_UTR 55.0%
! TGCTCTTGCTGCTTCAGGTC+AGG - chr4_3:25049135-25049154 Msa0635730:CDS 55.0%
!! TGGACTGGTAGTGCTGGCTT+GGG + chr4_3:25049427-25049446 None:intergenic 55.0%
TCCACCTGGCTAACCCAAGT+GGG - chr4_3:25049443-25049462 Msa0635730:intron 55.0%
TTGGGTTTGGCTGTGTAGGC+CGG + chr4_3:25050474-25050493 None:intergenic 55.0%
GAGGTTGGGTTTGGCTGTGT+AGG + chr4_3:25050478-25050497 None:intergenic 55.0%
ACAGCCAAACCCAACCTCCA+AGG - chr4_3:25050480-25050499 Msa0635730:intron 55.0%
TTTGGGCCAACAGGGACTGT+GGG + chr4_3:25050506-25050525 None:intergenic 55.0%
ATTTGGGCCAACAGGGACTG+TGG + chr4_3:25050507-25050526 None:intergenic 55.0%
CAGTCCCTGTTGGCCCAAAT+TGG - chr4_3:25050507-25050526 Msa0635730:intron 55.0%
!! GTAGTGCTGGCTTGGGACCT+TGG + chr4_3:25049420-25049439 None:intergenic 60.0%
!! GTGGACTGGTAGTGCTGGCT+TGG + chr4_3:25049428-25049447 None:intergenic 60.0%
!! CTTGGGTTAGCCAGGTGGAC+TGG + chr4_3:25049442-25049461 None:intergenic 60.0%
GTCCACCTGGCTAACCCAAG+TGG - chr4_3:25049442-25049461 Msa0635730:intron 60.0%
!! ACCCACTTGGGTTAGCCAGG+TGG + chr4_3:25049447-25049466 None:intergenic 60.0%
TGGCTGTGTAGGCCGGACTT+GGG + chr4_3:25050467-25050486 None:intergenic 60.0%
TTGGCTGTGTAGGCCGGACT+TGG + chr4_3:25050468-25050487 None:intergenic 60.0%
TGGGCCTTGGAGGTTGGGTT+TGG + chr4_3:25050487-25050506 None:intergenic 60.0%
GACTGTGGGCCTTGGAGGTT+GGG + chr4_3:25050492-25050511 None:intergenic 60.0%
GCCAGCACTACCAGTCCACC+TGG - chr4_3:25049429-25049448 Msa0635730:intron 65.0%
!! GCCAGGTGGACTGGTAGTGC+TGG + chr4_3:25049433-25049452 None:intergenic 65.0%
GGACTGTGGGCCTTGGAGGT+TGG + chr4_3:25050493-25050512 None:intergenic 65.0%
ACAGGGACTGTGGGCCTTGG+AGG + chr4_3:25050497-25050516 None:intergenic 65.0%
CCAACAGGGACTGTGGGCCT+TGG + chr4_3:25050500-25050519 None:intergenic 65.0%
CCAAGGCCCACAGTCCCTGT+TGG - chr4_3:25050497-25050516 Msa0635730:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 25048714 25051880 25048714 ID=Msa0635730;Name=Msa0635730
chr4_3 mRNA 25048714 25051880 25048714 ID=Msa0635730-mRNA-1;Parent=Msa0635730;Name=Msa0635730-mRNA-1;_AED=0.03;_eAED=0.03;_QI=80|1|1|1|1|1|2|336|167
chr4_3 exon 25048714 25049195 25048714 ID=Msa0635730-mRNA-1:exon:6334;Parent=Msa0635730-mRNA-1
chr4_3 exon 25051443 25051880 25051443 ID=Msa0635730-mRNA-1:exon:6333;Parent=Msa0635730-mRNA-1
chr4_3 five_prime_UTR 25051801 25051880 25051801 ID=Msa0635730-mRNA-1:five_prime_utr;Parent=Msa0635730-mRNA-1
chr4_3 CDS 25051443 25051800 25051443 ID=Msa0635730-mRNA-1:cds;Parent=Msa0635730-mRNA-1
chr4_3 CDS 25049050 25049195 25049050 ID=Msa0635730-mRNA-1:cds;Parent=Msa0635730-mRNA-1
chr4_3 three_prime_UTR 25048714 25049049 25048714 ID=Msa0635730-mRNA-1:three_prime_utr;Parent=Msa0635730-mRNA-1
Gene Sequence

>Msa0635730

ATGGCGCTACAAACACAATTCGTAACTTCCTTCCCCACCAAACTACATCACTCATTCTCTCCTATCTTCACAATCAAGTCCGAAAAACCTTCTGAACCGTCCGATTCAGTTCCCAACAAAACTGGTGTGGGTTTTGGTCCGTCGAATGGCAACAACACTAAACGGAAACAGAAAGGGCAAAGAGAAAGAGCTTCCATAATTCGGCGTAATCCAGTTGAAAAACCCGCTTTCGTTTCGGAACAATTGCAACAACAACAACAAGTTCAAGAAGAGAGTGCTGTTGAAAAAGGTTTTATTGTTGCTTGGCTTGGTTTTGGTTCGGTTATTCTTGTTGAGGGTATTGCTCTTGCTGCTTCAGGCTTTTTTCCAGAAGAGTGGGACAATTTCCTTGTTAAATATCTCTATCCATCCTTTACCCCTACAGTATTATTGTTTCTTGCTGGAGCAGTAGCATTTGGAGTGGTGAAATATAAGCAGAATGAGAAACTCACGGAGCGAAAATAG
Protein sequence

>Msa0635730

MALQTQFVTSFPTKLHHSFSPIFTIKSEKPSEPSDSVPNKTGVGFGPSNGNNTKRKQKGQRERASIIRRNPVEKPAFVSEQLQQQQQVQEESAVEKGFIVAWLGFGSVILVEGIALAASGFFPEEWDNFLVKYLYPSFTPTVLLFLAGAVAFGVVKYKQNEKLTERK*