AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0639590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCCGTATGTTGTTTAATA+TGG 0.173919 4_3:-35990443 Msa0639590:three_prime_UTR
TTTATGTGTGAATCAGTTTC+AGG 0.211665 4_3:-35991211 Msa0639590:intron
CTGCAATTTAGTTGATAAAT+TGG 0.212035 4_3:-35991100 Msa0639590:intron
ATCTGTCTTACGCTTCTTAT+TGG 0.223158 4_3:+35991539 None:intergenic
GAGATGCTGTTCATTACTAT+TGG 0.250264 4_3:-35991778 Msa0639590:five_prime_UTR
GAAACTGATTCACACATAAA+AGG 0.287899 4_3:+35991214 None:intergenic
CATTACAGTTGGATACTATT+AGG 0.321806 4_3:+35991656 None:intergenic
TGATACTTGTTATATCTTGA+TGG 0.324900 4_3:+35990514 None:intergenic
ACAAACTTTCCAGGAGCAAC+TGG 0.333325 4_3:+35991337 None:intergenic
TCTGTCTTACGCTTCTTATT+GGG 0.333660 4_3:+35991540 None:intergenic
CACATACCATGAACATTCTC+TGG 0.335594 4_3:+35990958 None:intergenic
AGATGCTGTTCATTACTATT+GGG 0.336429 4_3:-35991777 Msa0639590:five_prime_UTR
AAACTCAAAGCAAGTAATAA+TGG 0.339664 4_3:+35991593 None:intergenic
TCCTACGTTTATGTGGATTA+TGG 0.343276 4_3:+35991257 None:intergenic
TGCATAAATAACATGCAATA+AGG 0.344550 4_3:+35990364 None:intergenic
GCTCTCATCGTCATCTATAT+TGG 0.350848 4_3:+35990572 None:intergenic
CTTGTTAAAACAAACTTTCC+AGG 0.351311 4_3:+35991328 None:intergenic
AATAAGGTAATTGAATAATA+AGG 0.355295 4_3:+35990380 None:intergenic
TGTGGATTATGGAGCTGATA+AGG 0.364663 4_3:+35991268 None:intergenic
TTGAATTACAACTGATGTAT+AGG 0.365130 4_3:-35992087 Msa0639590:five_prime_UTR
CATCGTCATCTATATTGGTA+TGG 0.375410 4_3:+35990577 None:intergenic
CCAGTCACTGGCATTCAGTT+TGG 0.376606 4_3:+35991959 None:intergenic
ATTTAATCCTTGGGTACAAT+TGG 0.377115 4_3:+35992025 None:intergenic
GTGGATTATGGAGCTGATAA+GGG 0.378232 4_3:+35991269 None:intergenic
CGGATTTCCCCAGTTGCTCC+TGG 0.380237 4_3:-35991346 Msa0639590:five_prime_UTR
AAGAAGCGTAAGACAGATAA+TGG 0.385122 4_3:-35991534 Msa0639590:five_prime_UTR
TCGTATAGGTCGTACATTAC+TGG 0.386396 4_3:-35991305 Msa0639590:five_prime_UTR
ACAGCATCTCAATTAATTAG+AGG 0.387983 4_3:+35991791 None:intergenic
ATCTTTGTGCCAACAGTTCA+CGG 0.398149 4_3:-35990864 Msa0639590:CDS
TGTGCTTGTGTCTTAGCATA+AGG 0.415041 4_3:+35991626 None:intergenic
CCAAACTGAATGCCAGTGAC+TGG 0.437911 4_3:-35991959 Msa0639590:five_prime_UTR
AACAAACGCATCAGGATTAG+AGG 0.443721 4_3:+35990539 None:intergenic
CTATATTGCTCATCATCATA+TGG 0.450830 4_3:-35990819 Msa0639590:CDS
TTGTTGCTCCTACGTTTATG+TGG 0.459705 4_3:+35991250 None:intergenic
TGGATTTAACAATTCGCAAC+CGG 0.460380 4_3:-35990799 Msa0639590:CDS
CAACTGGGGAAATCCGAGTT+AGG 0.461442 4_3:+35991353 None:intergenic
CCTGCAGTAGTCTGCATCTC+CGG 0.470823 4_3:+35990780 None:intergenic
ATTGCACTTCTCCCAGTCAC+TGG 0.476981 4_3:+35991947 None:intergenic
TGATGGATAACAAACGCATC+AGG 0.485891 4_3:+35990531 None:intergenic
GATGTCCCAGAGAATGTTCA+TGG 0.486755 4_3:-35990964 Msa0639590:intron
TGAAACTATCAGAACAATAC+AGG 0.500217 4_3:-35991571 Msa0639590:five_prime_UTR
TTGTAAATTGCGGCAACGTA+GGG 0.500368 4_3:+35990658 None:intergenic
ACGCATCAGGATTAGAGGCT+TGG 0.501730 4_3:+35990544 None:intergenic
TATCAACAATGCAATGAGAA+TGG 0.503573 4_3:-35990997 Msa0639590:CDS
CACTATCGTACAGTCAGCCT+TGG 0.506626 4_3:+35991065 None:intergenic
CTACAAAATCCCACCTAACT+CGG 0.507570 4_3:-35991366 Msa0639590:five_prime_UTR
CATCCATATTAAACAACATA+CGG 0.510492 4_3:+35990440 None:intergenic
TACACCTGAAAACAGAAGAA+TGG 0.513898 4_3:+35990476 None:intergenic
ATCGTCATCTATATTGGTAT+GGG 0.519801 4_3:+35990578 None:intergenic
TTTGTAAATTGCGGCAACGT+AGG 0.528120 4_3:+35990657 None:intergenic
CGAAAATCTACAAGTATCGA+TGG 0.539917 4_3:-35990619 Msa0639590:CDS
CTGGGGAAATCCGAGTTAGG+TGG 0.566144 4_3:+35991356 None:intergenic
AACTTCATTGAATGCATGCA+AGG 0.568685 4_3:+35991828 None:intergenic
TGCTAATATCAGACAAAGGA+CGG 0.570993 4_3:+35991508 None:intergenic
AAGGACATCAGCATTACAGT+TGG 0.570996 4_3:+35991645 None:intergenic
CCGGAGATGCAGACTACTGC+AGG 0.577948 4_3:-35990780 Msa0639590:intron
TACGTTGCCGCAATTTACAA+AGG 0.580012 4_3:-35990655 Msa0639590:CDS
ATTGGAAGCAAATGCATCCA+AGG 0.582146 4_3:-35991082 Msa0639590:five_prime_UTR
CAAACTTTCCAGGAGCAACT+GGG 0.584286 4_3:+35991338 None:intergenic
CAAACTGAATGCCAGTGACT+GGG 0.587312 4_3:-35991958 Msa0639590:five_prime_UTR
TGAGGACAAACTATGATGTA+CGG 0.601800 4_3:-35991925 Msa0639590:five_prime_UTR
TGACTGGCCTTTGTAAATTG+CGG 0.603828 4_3:+35990648 None:intergenic
TGGGGAAATCCGAGTTAGGT+GGG 0.605109 4_3:+35991357 None:intergenic
ACATACCATGAACATTCTCT+GGG 0.606581 4_3:+35990959 None:intergenic
CCTTTGTCTGATATTAGCAA+CGG 0.609429 4_3:-35991504 Msa0639590:intron
CGTACATTACTGGACAAGAG+TGG 0.625871 4_3:-35991295 Msa0639590:five_prime_UTR
AAACTTTCCAGGAGCAACTG+GGG 0.631769 4_3:+35991339 None:intergenic
TGGATTATGGAGCTGATAAG+GGG 0.647552 4_3:+35991270 None:intergenic
AAATCTACAAGTATCGATGG+AGG 0.672434 4_3:-35990616 Msa0639590:CDS
TCCATAATCCACATAAACGT+AGG 0.685181 4_3:-35991258 Msa0639590:five_prime_UTR
AAGCGTAAGACAGATAATGG+TGG 0.715023 4_3:-35991531 Msa0639590:five_prime_UTR
TGACTGGGAGAAGTGCAATG+AGG 0.718853 4_3:-35991943 Msa0639590:five_prime_UTR
CCGTTGCTAATATCAGACAA+AGG 0.729869 4_3:+35991504 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATCATATTTTATATATTAA+TGG - chr4_3:35991556-35991575 Msa0639590:five_prime_UTR 10.0%
!!! GATCATATTTTATATATTAA+TGG - chr4_3:35991556-35991575 Msa0639590:five_prime_UTR 10.0%
!!! TGATATTTTATTTAATCCTT+GGG + chr4_3:35990440-35990459 None:intergenic 15.0%
!!! ATGATATTTTATTTAATCCT+TGG + chr4_3:35990441-35990460 None:intergenic 15.0%
!!! TATAACAAGTATCATATTTT+TGG - chr4_3:35991948-35991967 Msa0639590:five_prime_UTR 15.0%
!! AATAAGGTAATTGAATAATA+AGG + chr4_3:35992076-35992095 None:intergenic 15.0%
!!! TGATATTTTATTTAATCCTT+GGG + chr4_3:35990440-35990459 None:intergenic 15.0%
!!! ATGATATTTTATTTAATCCT+TGG + chr4_3:35990441-35990460 None:intergenic 15.0%
!!! TATAACAAGTATCATATTTT+TGG - chr4_3:35991948-35991967 Msa0639590:five_prime_UTR 15.0%
!! AATAAGGTAATTGAATAATA+AGG + chr4_3:35992076-35992095 None:intergenic 15.0%
!!! ATTTTATCTTTCACATAAGT+TGG - chr4_3:35990739-35990758 Msa0639590:intron 20.0%
!!! TTTTATCTTTCACATAAGTT+GGG - chr4_3:35990740-35990759 Msa0639590:intron 20.0%
!!! ATTTTATCTTTCACATAAGT+TGG - chr4_3:35990739-35990758 Msa0639590:intron 20.0%
!!! TTTTATCTTTCACATAAGTT+GGG - chr4_3:35990740-35990759 Msa0639590:intron 20.0%
! TTGAATTACAACTGATGTAT+AGG - chr4_3:35990366-35990385 Msa0639590:three_prime_UTR 25.0%
! AAACTCAAAGCAAGTAATAA+TGG + chr4_3:35990863-35990882 None:intergenic 25.0%
! AAACAGTATAAGATAGTTCT+GGG + chr4_3:35991007-35991026 None:intergenic 25.0%
!!! TTTGTTTTAACAAGTCGTAT+AGG - chr4_3:35991134-35991153 Msa0639590:intron 25.0%
!!! CTGCAATTTAGTTGATAAAT+TGG - chr4_3:35991353-35991372 Msa0639590:five_prime_UTR 25.0%
! ATGATCAATGCAAATGTTAT+AGG + chr4_3:35991542-35991561 None:intergenic 25.0%
! CCTACAGACTTATTAAAATA+TGG + chr4_3:35991726-35991745 None:intergenic 25.0%
!!! CCATATTTTAATAAGTCTGT+AGG - chr4_3:35991723-35991742 Msa0639590:five_prime_UTR 25.0%
!!! TGATACTTGTTATATCTTGA+TGG + chr4_3:35991942-35991961 None:intergenic 25.0%
! CATCCATATTAAACAACATA+CGG + chr4_3:35992016-35992035 None:intergenic 25.0%
! TGCATAAATAACATGCAATA+AGG + chr4_3:35992092-35992111 None:intergenic 25.0%
! ATTCAATAGCAAAACTATCA+CGG + chr4_3:35990352-35990371 None:intergenic 25.0%
! TTGAATTACAACTGATGTAT+AGG - chr4_3:35990366-35990385 Msa0639590:three_prime_UTR 25.0%
! AAACTCAAAGCAAGTAATAA+TGG + chr4_3:35990863-35990882 None:intergenic 25.0%
! AAACAGTATAAGATAGTTCT+GGG + chr4_3:35991007-35991026 None:intergenic 25.0%
!!! TTTGTTTTAACAAGTCGTAT+AGG - chr4_3:35991134-35991153 Msa0639590:intron 25.0%
!!! CTGCAATTTAGTTGATAAAT+TGG - chr4_3:35991353-35991372 Msa0639590:five_prime_UTR 25.0%
! ATGATCAATGCAAATGTTAT+AGG + chr4_3:35991542-35991561 None:intergenic 25.0%
! CCTACAGACTTATTAAAATA+TGG + chr4_3:35991726-35991745 None:intergenic 25.0%
!!! CCATATTTTAATAAGTCTGT+AGG - chr4_3:35991723-35991742 Msa0639590:five_prime_UTR 25.0%
!!! TGATACTTGTTATATCTTGA+TGG + chr4_3:35991942-35991961 None:intergenic 25.0%
! CATCCATATTAAACAACATA+CGG + chr4_3:35992016-35992035 None:intergenic 25.0%
! TGCATAAATAACATGCAATA+AGG + chr4_3:35992092-35992111 None:intergenic 25.0%
!! ATTTTAACCAATTGTACCCA+AGG - chr4_3:35990421-35990440 Msa0639590:three_prime_UTR 30.0%
ATTTAATCCTTGGGTACAAT+TGG + chr4_3:35990431-35990450 None:intergenic 30.0%
ACAGCATCTCAATTAATTAG+AGG + chr4_3:35990665-35990684 None:intergenic 30.0%
AGATGCTGTTCATTACTATT+GGG - chr4_3:35990676-35990695 Msa0639590:intron 30.0%
CATTACAGTTGGATACTATT+AGG + chr4_3:35990800-35990819 None:intergenic 30.0%
TGAAACTATCAGAACAATAC+AGG - chr4_3:35990882-35990901 Msa0639590:intron 30.0%
CAAACAGTATAAGATAGTTC+TGG + chr4_3:35991008-35991027 None:intergenic 30.0%
CTTGTTAAAACAAACTTTCC+AGG + chr4_3:35991128-35991147 None:intergenic 30.0%
! GAAACTGATTCACACATAAA+AGG + chr4_3:35991242-35991261 None:intergenic 30.0%
TTTATGTGTGAATCAGTTTC+AGG - chr4_3:35991242-35991261 Msa0639590:five_prime_UTR 30.0%
TATCAACAATGCAATGAGAA+TGG - chr4_3:35991456-35991475 Msa0639590:intron 30.0%
CTATATTGCTCATCATCATA+TGG - chr4_3:35991634-35991653 Msa0639590:five_prime_UTR 30.0%
ATCGTCATCTATATTGGTAT+GGG + chr4_3:35991878-35991897 None:intergenic 30.0%
! AATTCCATTCTTCTGTTTTC+AGG - chr4_3:35991973-35991992 Msa0639590:five_prime_UTR 30.0%
! TAGCCGTATGTTGTTTAATA+TGG - chr4_3:35992010-35992029 Msa0639590:five_prime_UTR 30.0%
!! ATTTTAACCAATTGTACCCA+AGG - chr4_3:35990421-35990440 Msa0639590:three_prime_UTR 30.0%
ATTTAATCCTTGGGTACAAT+TGG + chr4_3:35990431-35990450 None:intergenic 30.0%
ACAGCATCTCAATTAATTAG+AGG + chr4_3:35990665-35990684 None:intergenic 30.0%
AGATGCTGTTCATTACTATT+GGG - chr4_3:35990676-35990695 Msa0639590:intron 30.0%
CATTACAGTTGGATACTATT+AGG + chr4_3:35990800-35990819 None:intergenic 30.0%
TGAAACTATCAGAACAATAC+AGG - chr4_3:35990882-35990901 Msa0639590:intron 30.0%
CAAACAGTATAAGATAGTTC+TGG + chr4_3:35991008-35991027 None:intergenic 30.0%
CTTGTTAAAACAAACTTTCC+AGG + chr4_3:35991128-35991147 None:intergenic 30.0%
! GAAACTGATTCACACATAAA+AGG + chr4_3:35991242-35991261 None:intergenic 30.0%
TTTATGTGTGAATCAGTTTC+AGG - chr4_3:35991242-35991261 Msa0639590:five_prime_UTR 30.0%
TATCAACAATGCAATGAGAA+TGG - chr4_3:35991456-35991475 Msa0639590:intron 30.0%
CTATATTGCTCATCATCATA+TGG - chr4_3:35991634-35991653 Msa0639590:five_prime_UTR 30.0%
ATCGTCATCTATATTGGTAT+GGG + chr4_3:35991878-35991897 None:intergenic 30.0%
! AATTCCATTCTTCTGTTTTC+AGG - chr4_3:35991973-35991992 Msa0639590:five_prime_UTR 30.0%
! TAGCCGTATGTTGTTTAATA+TGG - chr4_3:35992010-35992029 Msa0639590:five_prime_UTR 30.0%
TGAGGACAAACTATGATGTA+CGG - chr4_3:35990528-35990547 Msa0639590:CDS 35.0%
AACTTCATTGAATGCATGCA+AGG + chr4_3:35990628-35990647 None:intergenic 35.0%
GAGATGCTGTTCATTACTAT+TGG - chr4_3:35990675-35990694 Msa0639590:intron 35.0%
TCTGTCTTACGCTTCTTATT+GGG + chr4_3:35990916-35990935 None:intergenic 35.0%
ATCTGTCTTACGCTTCTTAT+TGG + chr4_3:35990917-35990936 None:intergenic 35.0%
! AAGAAGCGTAAGACAGATAA+TGG - chr4_3:35990919-35990938 Msa0639590:intron 35.0%
TGCTAATATCAGACAAAGGA+CGG + chr4_3:35990948-35990967 None:intergenic 35.0%
CCTTTGTCTGATATTAGCAA+CGG - chr4_3:35990949-35990968 Msa0639590:intron 35.0%
CTGGGTTATAGAACATAAAC+AGG + chr4_3:35990989-35991008 None:intergenic 35.0%
TCCATAATCCACATAAACGT+AGG - chr4_3:35991195-35991214 Msa0639590:intron 35.0%
! TCCTACGTTTATGTGGATTA+TGG + chr4_3:35991199-35991218 None:intergenic 35.0%
TTATCAACTAAATTGCAGCG+AGG + chr4_3:35991351-35991370 None:intergenic 35.0%
ACATACCATGAACATTCTCT+GGG + chr4_3:35991497-35991516 None:intergenic 35.0%
! TCATATTTTCCGTGAACTGT+TGG + chr4_3:35991601-35991620 None:intergenic 35.0%
TGGATTTAACAATTCGCAAC+CGG - chr4_3:35991654-35991673 Msa0639590:five_prime_UTR 35.0%
!! CGAAAATCTACAAGTATCGA+TGG - chr4_3:35991834-35991853 Msa0639590:five_prime_UTR 35.0%
!! AAATCTACAAGTATCGATGG+AGG - chr4_3:35991837-35991856 Msa0639590:five_prime_UTR 35.0%
CATCGTCATCTATATTGGTA+TGG + chr4_3:35991879-35991898 None:intergenic 35.0%
TACACCTGAAAACAGAAGAA+TGG + chr4_3:35991980-35991999 None:intergenic 35.0%
!!! TTCTTCTGTTTTCAGGTGTA+TGG - chr4_3:35991980-35991999 Msa0639590:five_prime_UTR 35.0%
TGAGGACAAACTATGATGTA+CGG - chr4_3:35990528-35990547 Msa0639590:CDS 35.0%
AACTTCATTGAATGCATGCA+AGG + chr4_3:35990628-35990647 None:intergenic 35.0%
GAGATGCTGTTCATTACTAT+TGG - chr4_3:35990675-35990694 Msa0639590:intron 35.0%
TCTGTCTTACGCTTCTTATT+GGG + chr4_3:35990916-35990935 None:intergenic 35.0%
ATCTGTCTTACGCTTCTTAT+TGG + chr4_3:35990917-35990936 None:intergenic 35.0%
! AAGAAGCGTAAGACAGATAA+TGG - chr4_3:35990919-35990938 Msa0639590:intron 35.0%
TGCTAATATCAGACAAAGGA+CGG + chr4_3:35990948-35990967 None:intergenic 35.0%
CCTTTGTCTGATATTAGCAA+CGG - chr4_3:35990949-35990968 Msa0639590:intron 35.0%
CTGGGTTATAGAACATAAAC+AGG + chr4_3:35990989-35991008 None:intergenic 35.0%
TCCATAATCCACATAAACGT+AGG - chr4_3:35991195-35991214 Msa0639590:intron 35.0%
! TCCTACGTTTATGTGGATTA+TGG + chr4_3:35991199-35991218 None:intergenic 35.0%
TTATCAACTAAATTGCAGCG+AGG + chr4_3:35991351-35991370 None:intergenic 35.0%
ACATACCATGAACATTCTCT+GGG + chr4_3:35991497-35991516 None:intergenic 35.0%
! TCATATTTTCCGTGAACTGT+TGG + chr4_3:35991601-35991620 None:intergenic 35.0%
TGGATTTAACAATTCGCAAC+CGG - chr4_3:35991654-35991673 Msa0639590:five_prime_UTR 35.0%
!! CGAAAATCTACAAGTATCGA+TGG - chr4_3:35991834-35991853 Msa0639590:five_prime_UTR 35.0%
!! AAATCTACAAGTATCGATGG+AGG - chr4_3:35991837-35991856 Msa0639590:five_prime_UTR 35.0%
CATCGTCATCTATATTGGTA+TGG + chr4_3:35991879-35991898 None:intergenic 35.0%
TACACCTGAAAACAGAAGAA+TGG + chr4_3:35991980-35991999 None:intergenic 35.0%
!!! TTCTTCTGTTTTCAGGTGTA+TGG - chr4_3:35991980-35991999 Msa0639590:five_prime_UTR 35.0%
AAGGACATCAGCATTACAGT+TGG + chr4_3:35990811-35990830 None:intergenic 40.0%
TGTGCTTGTGTCTTAGCATA+AGG + chr4_3:35990830-35990849 None:intergenic 40.0%
! AAGCGTAAGACAGATAATGG+TGG - chr4_3:35990922-35990941 Msa0639590:intron 40.0%
CCGTTGCTAATATCAGACAA+AGG + chr4_3:35990952-35990971 None:intergenic 40.0%
AACTGCAAAATCACGTACGT+AGG + chr4_3:35991039-35991058 None:intergenic 40.0%
CTACAAAATCCCACCTAACT+CGG - chr4_3:35991087-35991106 Msa0639590:five_prime_UTR 40.0%
TCGTATAGGTCGTACATTAC+TGG - chr4_3:35991148-35991167 Msa0639590:intron 40.0%
TGGATTATGGAGCTGATAAG+GGG + chr4_3:35991186-35991205 None:intergenic 40.0%
! GTGGATTATGGAGCTGATAA+GGG + chr4_3:35991187-35991206 None:intergenic 40.0%
! TGTGGATTATGGAGCTGATA+AGG + chr4_3:35991188-35991207 None:intergenic 40.0%
TTGTTGCTCCTACGTTTATG+TGG + chr4_3:35991206-35991225 None:intergenic 40.0%
! GTGTGAATCAGTTTCAGGTA+TGG - chr4_3:35991247-35991266 Msa0639590:five_prime_UTR 40.0%
! GAATCAGTTTCAGGTATGGT+TGG - chr4_3:35991251-35991270 Msa0639590:five_prime_UTR 40.0%
ATTGGAAGCAAATGCATCCA+AGG - chr4_3:35991371-35991390 Msa0639590:five_prime_UTR 40.0%
CACATACCATGAACATTCTC+TGG + chr4_3:35991498-35991517 None:intergenic 40.0%
CAGAGAATGTTCATGGTATG+TGG - chr4_3:35991496-35991515 Msa0639590:intron 40.0%
ATCTTTGTGCCAACAGTTCA+CGG - chr4_3:35991589-35991608 Msa0639590:five_prime_UTR 40.0%
TTGTAAATTGCGGCAACGTA+GGG + chr4_3:35991798-35991817 None:intergenic 40.0%
TTTGTAAATTGCGGCAACGT+AGG + chr4_3:35991799-35991818 None:intergenic 40.0%
TACGTTGCCGCAATTTACAA+AGG - chr4_3:35991798-35991817 Msa0639590:five_prime_UTR 40.0%
!! TGACTGGCCTTTGTAAATTG+CGG + chr4_3:35991808-35991827 None:intergenic 40.0%
! TAGATTTTCGCAGAGATGAC+TGG + chr4_3:35991824-35991843 None:intergenic 40.0%
GCTCTCATCGTCATCTATAT+TGG + chr4_3:35991884-35991903 None:intergenic 40.0%
AACAAACGCATCAGGATTAG+AGG + chr4_3:35991917-35991936 None:intergenic 40.0%
! TGATGGATAACAAACGCATC+AGG + chr4_3:35991925-35991944 None:intergenic 40.0%
AAGGACATCAGCATTACAGT+TGG + chr4_3:35990811-35990830 None:intergenic 40.0%
TGTGCTTGTGTCTTAGCATA+AGG + chr4_3:35990830-35990849 None:intergenic 40.0%
! AAGCGTAAGACAGATAATGG+TGG - chr4_3:35990922-35990941 Msa0639590:intron 40.0%
CCGTTGCTAATATCAGACAA+AGG + chr4_3:35990952-35990971 None:intergenic 40.0%
AACTGCAAAATCACGTACGT+AGG + chr4_3:35991039-35991058 None:intergenic 40.0%
CTACAAAATCCCACCTAACT+CGG - chr4_3:35991087-35991106 Msa0639590:five_prime_UTR 40.0%
TCGTATAGGTCGTACATTAC+TGG - chr4_3:35991148-35991167 Msa0639590:intron 40.0%
TGGATTATGGAGCTGATAAG+GGG + chr4_3:35991186-35991205 None:intergenic 40.0%
! GTGGATTATGGAGCTGATAA+GGG + chr4_3:35991187-35991206 None:intergenic 40.0%
! TGTGGATTATGGAGCTGATA+AGG + chr4_3:35991188-35991207 None:intergenic 40.0%
TTGTTGCTCCTACGTTTATG+TGG + chr4_3:35991206-35991225 None:intergenic 40.0%
! GTGTGAATCAGTTTCAGGTA+TGG - chr4_3:35991247-35991266 Msa0639590:five_prime_UTR 40.0%
! GAATCAGTTTCAGGTATGGT+TGG - chr4_3:35991251-35991270 Msa0639590:five_prime_UTR 40.0%
ATTGGAAGCAAATGCATCCA+AGG - chr4_3:35991371-35991390 Msa0639590:five_prime_UTR 40.0%
CACATACCATGAACATTCTC+TGG + chr4_3:35991498-35991517 None:intergenic 40.0%
CAGAGAATGTTCATGGTATG+TGG - chr4_3:35991496-35991515 Msa0639590:intron 40.0%
ATCTTTGTGCCAACAGTTCA+CGG - chr4_3:35991589-35991608 Msa0639590:five_prime_UTR 40.0%
TTGTAAATTGCGGCAACGTA+GGG + chr4_3:35991798-35991817 None:intergenic 40.0%
TTTGTAAATTGCGGCAACGT+AGG + chr4_3:35991799-35991818 None:intergenic 40.0%
TACGTTGCCGCAATTTACAA+AGG - chr4_3:35991798-35991817 Msa0639590:five_prime_UTR 40.0%
!! TGACTGGCCTTTGTAAATTG+CGG + chr4_3:35991808-35991827 None:intergenic 40.0%
! TAGATTTTCGCAGAGATGAC+TGG + chr4_3:35991824-35991843 None:intergenic 40.0%
GCTCTCATCGTCATCTATAT+TGG + chr4_3:35991884-35991903 None:intergenic 40.0%
AACAAACGCATCAGGATTAG+AGG + chr4_3:35991917-35991936 None:intergenic 40.0%
! TGATGGATAACAAACGCATC+AGG + chr4_3:35991925-35991944 None:intergenic 40.0%
CAAACTGAATGCCAGTGACT+GGG - chr4_3:35990495-35990514 Msa0639590:CDS 45.0%
!! AGAGTTGCACGCTTTTAGGT+TGG + chr4_3:35990707-35990726 None:intergenic 45.0%
AAACTTTCCAGGAGCAACTG+GGG + chr4_3:35991117-35991136 None:intergenic 45.0%
CAAACTTTCCAGGAGCAACT+GGG + chr4_3:35991118-35991137 None:intergenic 45.0%
ACAAACTTTCCAGGAGCAAC+TGG + chr4_3:35991119-35991138 None:intergenic 45.0%
CGTACATTACTGGACAAGAG+TGG - chr4_3:35991158-35991177 Msa0639590:intron 45.0%
GATGTCCCAGAGAATGTTCA+TGG - chr4_3:35991489-35991508 Msa0639590:intron 45.0%
AGACTACTGCAGGTTAGGAA+TGG - chr4_3:35991683-35991702 Msa0639590:five_prime_UTR 45.0%
ACTGCTGTTGCAATCTCAGA+AGG - chr4_3:35991766-35991785 Msa0639590:five_prime_UTR 45.0%
CAAACTGAATGCCAGTGACT+GGG - chr4_3:35990495-35990514 Msa0639590:CDS 45.0%
!! AGAGTTGCACGCTTTTAGGT+TGG + chr4_3:35990707-35990726 None:intergenic 45.0%
AAACTTTCCAGGAGCAACTG+GGG + chr4_3:35991117-35991136 None:intergenic 45.0%
CAAACTTTCCAGGAGCAACT+GGG + chr4_3:35991118-35991137 None:intergenic 45.0%
ACAAACTTTCCAGGAGCAAC+TGG + chr4_3:35991119-35991138 None:intergenic 45.0%
CGTACATTACTGGACAAGAG+TGG - chr4_3:35991158-35991177 Msa0639590:intron 45.0%
GATGTCCCAGAGAATGTTCA+TGG - chr4_3:35991489-35991508 Msa0639590:intron 45.0%
AGACTACTGCAGGTTAGGAA+TGG - chr4_3:35991683-35991702 Msa0639590:five_prime_UTR 45.0%
ACTGCTGTTGCAATCTCAGA+AGG - chr4_3:35991766-35991785 Msa0639590:five_prime_UTR 45.0%
!!! TTATATTTTTTTATTTACAT+TGG - chr4_3:35990564-35990583 Msa0639590:CDS 5.0%
!!! ATCATATTTTATATATTAAT+GGG - chr4_3:35991557-35991576 Msa0639590:five_prime_UTR 5.0%
!!! TTATATTTTTTTATTTACAT+TGG - chr4_3:35990564-35990583 Msa0639590:CDS 5.0%
!!! ATCATATTTTATATATTAAT+GGG - chr4_3:35991557-35991576 Msa0639590:five_prime_UTR 5.0%
CCAGTCACTGGCATTCAGTT+TGG + chr4_3:35990497-35990516 None:intergenic 50.0%
CCAAACTGAATGCCAGTGAC+TGG - chr4_3:35990494-35990513 Msa0639590:CDS 50.0%
ATTGCACTTCTCCCAGTCAC+TGG + chr4_3:35990509-35990528 None:intergenic 50.0%
! TGACTGGGAGAAGTGCAATG+AGG - chr4_3:35990510-35990529 Msa0639590:CDS 50.0%
! GCACAGAGTTGCACGCTTTT+AGG + chr4_3:35990711-35990730 None:intergenic 50.0%
TGGGGAAATCCGAGTTAGGT+GGG + chr4_3:35991099-35991118 None:intergenic 50.0%
CAACTGGGGAAATCCGAGTT+AGG + chr4_3:35991103-35991122 None:intergenic 50.0%
CACTATCGTACAGTCAGCCT+TGG + chr4_3:35991391-35991410 None:intergenic 50.0%
GATGCAGACTACTGCAGGTT+AGG - chr4_3:35991678-35991697 Msa0639590:five_prime_UTR 50.0%
ACGCATCAGGATTAGAGGCT+TGG + chr4_3:35991912-35991931 None:intergenic 50.0%
CCAGTCACTGGCATTCAGTT+TGG + chr4_3:35990497-35990516 None:intergenic 50.0%
CCAAACTGAATGCCAGTGAC+TGG - chr4_3:35990494-35990513 Msa0639590:CDS 50.0%
ATTGCACTTCTCCCAGTCAC+TGG + chr4_3:35990509-35990528 None:intergenic 50.0%
! TGACTGGGAGAAGTGCAATG+AGG - chr4_3:35990510-35990529 Msa0639590:CDS 50.0%
! GCACAGAGTTGCACGCTTTT+AGG + chr4_3:35990711-35990730 None:intergenic 50.0%
TGGGGAAATCCGAGTTAGGT+GGG + chr4_3:35991099-35991118 None:intergenic 50.0%
CAACTGGGGAAATCCGAGTT+AGG + chr4_3:35991103-35991122 None:intergenic 50.0%
CACTATCGTACAGTCAGCCT+TGG + chr4_3:35991391-35991410 None:intergenic 50.0%
GATGCAGACTACTGCAGGTT+AGG - chr4_3:35991678-35991697 Msa0639590:five_prime_UTR 50.0%
ACGCATCAGGATTAGAGGCT+TGG + chr4_3:35991912-35991931 None:intergenic 50.0%
CTGGGGAAATCCGAGTTAGG+TGG + chr4_3:35991100-35991119 None:intergenic 55.0%
CCTGCAGTAGTCTGCATCTC+CGG + chr4_3:35991676-35991695 None:intergenic 55.0%
CTGGGGAAATCCGAGTTAGG+TGG + chr4_3:35991100-35991119 None:intergenic 55.0%
CCTGCAGTAGTCTGCATCTC+CGG + chr4_3:35991676-35991695 None:intergenic 55.0%
CGGATTTCCCCAGTTGCTCC+TGG - chr4_3:35991107-35991126 Msa0639590:intron 60.0%
CCGGAGATGCAGACTACTGC+AGG - chr4_3:35991673-35991692 Msa0639590:five_prime_UTR 60.0%
CGGATTTCCCCAGTTGCTCC+TGG - chr4_3:35991107-35991126 Msa0639590:intron 60.0%
CCGGAGATGCAGACTACTGC+AGG - chr4_3:35991673-35991692 Msa0639590:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 35990352 35992123 35990352 ID=Msa0639590;Name=Msa0639590
chr4_3 mRNA 35990352 35992123 35990352 ID=Msa0639590-mRNA-1;Parent=Msa0639590;Name=Msa0639590-mRNA-1;_AED=0.05;_eAED=0.05;_QI=895|0.75|0.8|1|0.75|0.8|5|118|136
chr4_3 exon 35990352 35990690 35990352 ID=Msa0639590-mRNA-1:exon:8362;Parent=Msa0639590-mRNA-1
chr4_3 exon 35990781 35990892 35990781 ID=Msa0639590-mRNA-1:exon:8361;Parent=Msa0639590-mRNA-1
chr4_3 exon 35990965 35991111 35990965 ID=Msa0639590-mRNA-1:exon:8360;Parent=Msa0639590-mRNA-1
chr4_3 exon 35991212 35991418 35991212 ID=Msa0639590-mRNA-1:exon:8359;Parent=Msa0639590-mRNA-1
chr4_3 exon 35991505 35992123 35991505 ID=Msa0639590-mRNA-1:exon:8358;Parent=Msa0639590-mRNA-1
chr4_3 five_prime_UTR 35991505 35992123 35991505 ID=Msa0639590-mRNA-1:five_prime_utr;Parent=Msa0639590-mRNA-1
chr4_3 five_prime_UTR 35991212 35991418 35991212 ID=Msa0639590-mRNA-1:five_prime_utr;Parent=Msa0639590-mRNA-1
chr4_3 five_prime_UTR 35991043 35991111 35991043 ID=Msa0639590-mRNA-1:five_prime_utr;Parent=Msa0639590-mRNA-1
chr4_3 CDS 35990965 35991042 35990965 ID=Msa0639590-mRNA-1:cds;Parent=Msa0639590-mRNA-1
chr4_3 CDS 35990781 35990892 35990781 ID=Msa0639590-mRNA-1:cds;Parent=Msa0639590-mRNA-1
chr4_3 CDS 35990470 35990690 35990470 ID=Msa0639590-mRNA-1:cds;Parent=Msa0639590-mRNA-1
chr4_3 three_prime_UTR 35990352 35990469 35990352 ID=Msa0639590-mRNA-1:three_prime_utr;Parent=Msa0639590-mRNA-1
Gene Sequence

>Msa0639590

ATGAAGAGAATAGCGAAAGCAAATATCAACAATGCAATGAGAATGGTGAAGATGACGATGTCCCAGAGAATGTTCATGTTGAAAATCTTTGTGCCAACAGTTCACGGAAAATATGATTTAGTTAAAGAACTATATTGCTCATCATCATATGGATTTAACAATTCGCAACCGGAGATGCAGACTACTGCAGAAGGTGCATTCCCTACGTTGCCGCAATTTACAAAGGCCAGTCATCTCTGCGAAAATCTACAAGTATCGATGGAGGATGCTTGCAAAACGACCCATACCAATATAGATGACGATGAGAGCTTGCACCAAGCCTCTAATCCTGATGCGTTTGTTATCCATCAAGATATAACAAGTATCATATTTTTGGTTAATTCCATTCTTCTGTTTTCAGGTGTATGGTAA
Protein sequence

>Msa0639590

MKRIAKANINNAMRMVKMTMSQRMFMLKIFVPTVHGKYDLVKELYCSSSYGFNNSQPEMQTTAEGAFPTLPQFTKASHLCENLQVSMEDACKTTHTNIDDDESLHQASNPDAFVIHQDITSIIFLVNSILLFSGVW*