AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0652240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0652240 MtrunA17_Chr4g0045791 98.522 203 3 0 1 203 1 203 8.48e-136 416
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0652240 AT3G59770.1 66.502 203 68 0 1 203 3 205 3.32e-93 298
Msa0652240 AT3G59770.3 66.502 203 68 0 1 203 3 205 3.61e-93 298

Find 66 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAATCTACTTGCATAAA+TGG 0.190628 4_3:+59956721 Msa0652240:three_prime_UTR
TCGTTGCAAATGTGGGATTT+TGG 0.234539 4_3:-59955893 None:intergenic
ATATTAGGTTACGCTGCTTT+AGG 0.283629 4_3:+59956337 Msa0652240:CDS
ATTCTTGGTAGAGCTATATT+AGG 0.295893 4_3:+59956322 Msa0652240:CDS
ACATTGTGTTCATCTCTTGC+AGG 0.309332 4_3:+59956675 Msa0652240:CDS
GCCGATTTGAGAGCTGTTCT+TGG 0.329420 4_3:-59955943 None:intergenic
AGCCTGATCCGGAGTTTGTT+TGG 0.349092 4_3:+59956608 Msa0652240:CDS
TGGCGGAGGTTGTGTTTATA+CGG 0.357918 4_3:+59956417 Msa0652240:CDS
GAGGTTGTGTTTATACGGTT+GGG 0.388052 4_3:+59956422 Msa0652240:CDS
CAACCTCCGCCAGGTAAATT+CGG 0.396649 4_3:-59956406 None:intergenic
TGATGAAACCACTCGTGAAA+TGG 0.402538 4_3:-59955974 None:intergenic
TCTACTTGCATAAATGGTAT+AGG 0.403032 4_3:+59956727 Msa0652240:three_prime_UTR
TTGTTAGAGCTTGACATTGA+TGG 0.409188 4_3:+59956520 Msa0652240:CDS
GGAGGTTGTGTTTATACGGT+TGG 0.417970 4_3:+59956421 Msa0652240:CDS
GGTTGCAAGAGTAGGATTCT+TGG 0.421641 4_3:+59956307 Msa0652240:CDS
TCTACGCCGTTTGTTAATGT+TGG 0.435171 4_3:+59956643 Msa0652240:CDS
TTCCAAACAAACTCCGGATC+AGG 0.435252 4_3:-59956610 None:intergenic
TTGCATAAATGGTATAGGTT+CGG 0.449975 4_3:+59956732 Msa0652240:three_prime_UTR
GTTACAAACGGATCGCGAAC+CGG 0.452075 4_3:+59956286 Msa0652240:CDS
CGCTTCGTAACGGAGGATTC+CGG 0.452102 4_3:-59956215 None:intergenic
GCCGTTTGTTAATGTTGGAT+TGG 0.455607 4_3:+59956648 Msa0652240:CDS
GTTGCAAAATGCACAGGTGC+AGG 0.458878 4_3:+59956468 Msa0652240:CDS
AGGTGAGGTGAAGAATGTTA+TGG 0.462488 4_3:+59956495 Msa0652240:CDS
TGTTAGAGCTTGACATTGAT+GGG 0.477618 4_3:+59956521 Msa0652240:CDS
ACCAAGAACAGCTCTCAAAT+CGG 0.478341 4_3:+59955942 Msa0652240:five_prime_UTR
GGCGGTTAATCAGCCTGATC+CGG 0.479465 4_3:+59956597 Msa0652240:CDS
TAACGGAGGATTCCGGTGGT+TGG 0.488165 4_3:-59956208 None:intergenic
AAGAATCCTACTCTTGCAAC+CGG 0.490206 4_3:-59956305 None:intergenic
ATTCTGGAAATAAACGAGTC+TGG 0.490996 4_3:+59955839 Msa0652240:five_prime_UTR
AGGTTGTGTTTATACGGTTG+GGG 0.492767 4_3:+59956423 Msa0652240:CDS
TCGTCCGTTTCCGAGTAGAA+TGG 0.497228 4_3:+59956576 Msa0652240:CDS
TTGTTACCTTGTGATTCCAT+CGG 0.507453 4_3:-59956004 None:intergenic
GCGAGTATTCCGAATTTACC+TGG 0.510432 4_3:+59956397 Msa0652240:CDS
ATAAACGAGTCTGGTTAAAA+CGG 0.515063 4_3:+59955848 Msa0652240:five_prime_UTR
GCCAATCCAACATTAACAAA+CGG 0.520526 4_3:-59956649 None:intergenic
ATCAAAATCCATTTCACGAG+TGG 0.533009 4_3:+59955966 Msa0652240:five_prime_UTR
GTGTATCCGTTCGTGTGCTT+AGG 0.533622 4_3:-59956168 None:intergenic
CGGAGTTTGTTTGGAATGCG+TGG 0.537599 4_3:+59956617 Msa0652240:CDS
AATGCACAGGTGCAGGGGAA+AGG 0.537658 4_3:+59956475 Msa0652240:CDS
GCTTACGAATTCGTTACAAA+CGG 0.537866 4_3:+59956274 Msa0652240:CDS
TACGATTATGTATACTTCGT+CGG 0.538807 4_3:-59956143 None:intergenic
CTCGTTTCGCTTCGTAACGG+AGG 0.539357 4_3:-59956222 None:intergenic
TTGCAAAATGCACAGGTGCA+GGG 0.544772 4_3:+59956469 Msa0652240:CDS
ACCGCCATTCTACTCGGAAA+CGG 0.548524 4_3:-59956580 None:intergenic
TGCAAAATGCACAGGTGCAG+GGG 0.551026 4_3:+59956470 Msa0652240:CDS
CGCGAACCGGTTGCAAGAGT+AGG 0.553705 4_3:+59956299 Msa0652240:CDS
ATTGAGTTCGTTGCAAATGT+GGG 0.554572 4_3:-59955900 None:intergenic
TTGGTTGTTAAGCTGTTGAG+AGG 0.561481 4_3:-59955924 None:intergenic
GATTCCGTTGCAAAATGCAC+AGG 0.562598 4_3:+59956462 Msa0652240:CDS
TGATTAACCGCCATTCTACT+CGG 0.562632 4_3:-59956586 None:intergenic
AACCTCGTTTCGCTTCGTAA+CGG 0.563242 4_3:-59956225 None:intergenic
AATTGAGTTCGTTGCAAATG+TGG 0.566730 4_3:-59955901 None:intergenic
CACGCATTCCAAACAAACTC+CGG 0.570212 4_3:-59956616 None:intergenic
GTTTCATCATCGTTAACCGA+TGG 0.571759 4_3:+59955988 Msa0652240:exon
CTCCGTTACGAAGCGAAACG+AGG 0.573432 4_3:+59956223 Msa0652240:CDS
ATACGGTTGGGGAGAGTCAG+TGG 0.575288 4_3:+59956434 Msa0652240:CDS
ATTCCGAATTTACCTGGCGG+AGG 0.583044 4_3:+59956403 Msa0652240:CDS
GTATAAACACAACCTCCGCC+AGG 0.594189 4_3:-59956415 None:intergenic
CGTTAACCGATGGAATCACA+AGG 0.642247 4_3:+59955998 Msa0652240:exon
TCCGTTTCCGAGTAGAATGG+CGG 0.646603 4_3:+59956579 Msa0652240:CDS
TTCGTAACGGAGGATTCCGG+TGG 0.648024 4_3:-59956212 None:intergenic
CTCTACGTAGATCCAACCAC+CGG 0.650575 4_3:+59956196 Msa0652240:CDS
CGTAAGCCTAAGCACACGAA+CGG 0.673665 4_3:+59956162 Msa0652240:CDS
ACAGGTGCAGGGGAAAGGTG+AGG 0.692165 4_3:+59956480 Msa0652240:CDS
ATCTAGCGTTACGACTACAA+CGG 0.741403 4_3:-59956119 None:intergenic
AGTATTCCGAATTTACCTGG+CGG 0.766404 4_3:+59956400 Msa0652240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTATTTCCAGAATTTTTT+TGG - chr4_3:59955834-59955853 None:intergenic 20.0%
!!! GTTTATTTCCAGAATTTTTT+TGG - chr4_3:59955834-59955853 None:intergenic 20.0%
!!! TATTTCCAGAATTTTTTTGG+AGG - chr4_3:59955831-59955850 None:intergenic 25.0%
!!! TATTCCTCTTTCTTTTTTTC+AGG + chr4_3:59956079-59956098 Msa0652240:intron 25.0%
! TTTCAATCTACTTGCATAAA+TGG + chr4_3:59956721-59956740 Msa0652240:three_prime_UTR 25.0%
!!! TATTTCCAGAATTTTTTTGG+AGG - chr4_3:59955831-59955850 None:intergenic 25.0%
!!! TATTCCTCTTTCTTTTTTTC+AGG + chr4_3:59956079-59956098 Msa0652240:intron 25.0%
! TTTCAATCTACTTGCATAAA+TGG + chr4_3:59956721-59956740 Msa0652240:three_prime_UTR 25.0%
ATAAACGAGTCTGGTTAAAA+CGG + chr4_3:59955848-59955867 Msa0652240:five_prime_UTR 30.0%
TACGATTATGTATACTTCGT+CGG - chr4_3:59956146-59956165 None:intergenic 30.0%
ATTCTTGGTAGAGCTATATT+AGG + chr4_3:59956322-59956341 Msa0652240:CDS 30.0%
TCTACTTGCATAAATGGTAT+AGG + chr4_3:59956727-59956746 Msa0652240:three_prime_UTR 30.0%
ATAAACGAGTCTGGTTAAAA+CGG + chr4_3:59955848-59955867 Msa0652240:five_prime_UTR 30.0%
TACGATTATGTATACTTCGT+CGG - chr4_3:59956146-59956165 None:intergenic 30.0%
ATTCTTGGTAGAGCTATATT+AGG + chr4_3:59956322-59956341 Msa0652240:CDS 30.0%
TCTACTTGCATAAATGGTAT+AGG + chr4_3:59956727-59956746 Msa0652240:three_prime_UTR 30.0%
!!! CCAGAATTTTTTTGGAGGAA+TGG - chr4_3:59955826-59955845 None:intergenic 35.0%
CCATTCCTCCAAAAAAATTC+TGG + chr4_3:59955823-59955842 Msa0652240:five_prime_UTR 35.0%
! ATTCTGGAAATAAACGAGTC+TGG + chr4_3:59955839-59955858 Msa0652240:five_prime_UTR 35.0%
! GATTTTGGACTAACGGAAAT+AGG - chr4_3:59955881-59955900 None:intergenic 35.0%
! AATGTGGGATTTTGGACTAA+CGG - chr4_3:59955888-59955907 None:intergenic 35.0%
ATTGAGTTCGTTGCAAATGT+GGG - chr4_3:59955903-59955922 None:intergenic 35.0%
AATTGAGTTCGTTGCAAATG+TGG - chr4_3:59955904-59955923 None:intergenic 35.0%
ATCAAAATCCATTTCACGAG+TGG + chr4_3:59955966-59955985 Msa0652240:five_prime_UTR 35.0%
TTGTTACCTTGTGATTCCAT+CGG - chr4_3:59956007-59956026 None:intergenic 35.0%
AAAAGAAAGAGGAATAAGCG+CGG - chr4_3:59956075-59956094 None:intergenic 35.0%
!!! TCCTCTTTCTTTTTTTCAGG+TGG + chr4_3:59956082-59956101 Msa0652240:intron 35.0%
TCCACCTGAAAAAAAGAAAG+AGG - chr4_3:59956086-59956105 None:intergenic 35.0%
GCTTACGAATTCGTTACAAA+CGG + chr4_3:59956274-59956293 Msa0652240:CDS 35.0%
! ATATTAGGTTACGCTGCTTT+AGG + chr4_3:59956337-59956356 Msa0652240:CDS 35.0%
TTGTTAGAGCTTGACATTGA+TGG + chr4_3:59956520-59956539 Msa0652240:CDS 35.0%
TGTTAGAGCTTGACATTGAT+GGG + chr4_3:59956521-59956540 Msa0652240:CDS 35.0%
!!! AGCATTATTTTTGTGAGACG+AGG + chr4_3:59956545-59956564 Msa0652240:CDS 35.0%
!!! GCATTATTTTTGTGAGACGA+GGG + chr4_3:59956546-59956565 Msa0652240:CDS 35.0%
GCCAATCCAACATTAACAAA+CGG - chr4_3:59956652-59956671 None:intergenic 35.0%
!!! CCAGAATTTTTTTGGAGGAA+TGG - chr4_3:59955826-59955845 None:intergenic 35.0%
CCATTCCTCCAAAAAAATTC+TGG + chr4_3:59955823-59955842 Msa0652240:five_prime_UTR 35.0%
! ATTCTGGAAATAAACGAGTC+TGG + chr4_3:59955839-59955858 Msa0652240:five_prime_UTR 35.0%
! GATTTTGGACTAACGGAAAT+AGG - chr4_3:59955881-59955900 None:intergenic 35.0%
! AATGTGGGATTTTGGACTAA+CGG - chr4_3:59955888-59955907 None:intergenic 35.0%
ATTGAGTTCGTTGCAAATGT+GGG - chr4_3:59955903-59955922 None:intergenic 35.0%
AATTGAGTTCGTTGCAAATG+TGG - chr4_3:59955904-59955923 None:intergenic 35.0%
ATCAAAATCCATTTCACGAG+TGG + chr4_3:59955966-59955985 Msa0652240:five_prime_UTR 35.0%
TTGTTACCTTGTGATTCCAT+CGG - chr4_3:59956007-59956026 None:intergenic 35.0%
AAAAGAAAGAGGAATAAGCG+CGG - chr4_3:59956075-59956094 None:intergenic 35.0%
!!! TCCTCTTTCTTTTTTTCAGG+TGG + chr4_3:59956082-59956101 Msa0652240:intron 35.0%
TCCACCTGAAAAAAAGAAAG+AGG - chr4_3:59956086-59956105 None:intergenic 35.0%
GCTTACGAATTCGTTACAAA+CGG + chr4_3:59956274-59956293 Msa0652240:CDS 35.0%
! ATATTAGGTTACGCTGCTTT+AGG + chr4_3:59956337-59956356 Msa0652240:CDS 35.0%
TTGTTAGAGCTTGACATTGA+TGG + chr4_3:59956520-59956539 Msa0652240:CDS 35.0%
TGTTAGAGCTTGACATTGAT+GGG + chr4_3:59956521-59956540 Msa0652240:CDS 35.0%
!!! AGCATTATTTTTGTGAGACG+AGG + chr4_3:59956545-59956564 Msa0652240:CDS 35.0%
!!! GCATTATTTTTGTGAGACGA+GGG + chr4_3:59956546-59956565 Msa0652240:CDS 35.0%
GCCAATCCAACATTAACAAA+CGG - chr4_3:59956652-59956671 None:intergenic 35.0%
! TCGTTGCAAATGTGGGATTT+TGG - chr4_3:59955896-59955915 None:intergenic 40.0%
TTGGTTGTTAAGCTGTTGAG+AGG - chr4_3:59955927-59955946 None:intergenic 40.0%
ACCAAGAACAGCTCTCAAAT+CGG + chr4_3:59955942-59955961 Msa0652240:five_prime_UTR 40.0%
TGATGAAACCACTCGTGAAA+TGG - chr4_3:59955977-59955996 None:intergenic 40.0%
GTTTCATCATCGTTAACCGA+TGG + chr4_3:59955988-59956007 Msa0652240:exon 40.0%
!!! GCGATTTTGATTGAGAAGTG+AGG - chr4_3:59956033-59956052 None:intergenic 40.0%
TAAGCGCGGTGAAATTAAGT+TGG - chr4_3:59956061-59956080 None:intergenic 40.0%
ATCTAGCGTTACGACTACAA+CGG - chr4_3:59956122-59956141 None:intergenic 40.0%
AAGAATCCTACTCTTGCAAC+CGG - chr4_3:59956308-59956327 None:intergenic 40.0%
! CTTTTCTTCTAATCGCAACG+AGG + chr4_3:59956368-59956387 Msa0652240:CDS 40.0%
AGTATTCCGAATTTACCTGG+CGG + chr4_3:59956400-59956419 Msa0652240:CDS 40.0%
GAGGTTGTGTTTATACGGTT+GGG + chr4_3:59956422-59956441 Msa0652240:CDS 40.0%
AGGTTGTGTTTATACGGTTG+GGG + chr4_3:59956423-59956442 Msa0652240:CDS 40.0%
AGGTGAGGTGAAGAATGTTA+TGG + chr4_3:59956495-59956514 Msa0652240:CDS 40.0%
TGATTAACCGCCATTCTACT+CGG - chr4_3:59956589-59956608 None:intergenic 40.0%
! TCTACGCCGTTTGTTAATGT+TGG + chr4_3:59956643-59956662 Msa0652240:CDS 40.0%
! GCCGTTTGTTAATGTTGGAT+TGG + chr4_3:59956648-59956667 Msa0652240:CDS 40.0%
ACATTGTGTTCATCTCTTGC+AGG + chr4_3:59956675-59956694 Msa0652240:CDS 40.0%
! TCGTTGCAAATGTGGGATTT+TGG - chr4_3:59955896-59955915 None:intergenic 40.0%
TTGGTTGTTAAGCTGTTGAG+AGG - chr4_3:59955927-59955946 None:intergenic 40.0%
ACCAAGAACAGCTCTCAAAT+CGG + chr4_3:59955942-59955961 Msa0652240:five_prime_UTR 40.0%
TGATGAAACCACTCGTGAAA+TGG - chr4_3:59955977-59955996 None:intergenic 40.0%
GTTTCATCATCGTTAACCGA+TGG + chr4_3:59955988-59956007 Msa0652240:exon 40.0%
!!! GCGATTTTGATTGAGAAGTG+AGG - chr4_3:59956033-59956052 None:intergenic 40.0%
TAAGCGCGGTGAAATTAAGT+TGG - chr4_3:59956061-59956080 None:intergenic 40.0%
ATCTAGCGTTACGACTACAA+CGG - chr4_3:59956122-59956141 None:intergenic 40.0%
AAGAATCCTACTCTTGCAAC+CGG - chr4_3:59956308-59956327 None:intergenic 40.0%
! CTTTTCTTCTAATCGCAACG+AGG + chr4_3:59956368-59956387 Msa0652240:CDS 40.0%
AGTATTCCGAATTTACCTGG+CGG + chr4_3:59956400-59956419 Msa0652240:CDS 40.0%
GAGGTTGTGTTTATACGGTT+GGG + chr4_3:59956422-59956441 Msa0652240:CDS 40.0%
AGGTTGTGTTTATACGGTTG+GGG + chr4_3:59956423-59956442 Msa0652240:CDS 40.0%
AGGTGAGGTGAAGAATGTTA+TGG + chr4_3:59956495-59956514 Msa0652240:CDS 40.0%
TGATTAACCGCCATTCTACT+CGG - chr4_3:59956589-59956608 None:intergenic 40.0%
! TCTACGCCGTTTGTTAATGT+TGG + chr4_3:59956643-59956662 Msa0652240:CDS 40.0%
! GCCGTTTGTTAATGTTGGAT+TGG + chr4_3:59956648-59956667 Msa0652240:CDS 40.0%
ACATTGTGTTCATCTCTTGC+AGG + chr4_3:59956675-59956694 Msa0652240:CDS 40.0%
CGTTAACCGATGGAATCACA+AGG + chr4_3:59955998-59956017 Msa0652240:exon 45.0%
AACCTCGTTTCGCTTCGTAA+CGG - chr4_3:59956228-59956247 None:intergenic 45.0%
GGTTGCAAGAGTAGGATTCT+TGG + chr4_3:59956307-59956326 Msa0652240:CDS 45.0%
GCGAGTATTCCGAATTTACC+TGG + chr4_3:59956397-59956416 Msa0652240:CDS 45.0%
TGGCGGAGGTTGTGTTTATA+CGG + chr4_3:59956417-59956436 Msa0652240:CDS 45.0%
GGAGGTTGTGTTTATACGGT+TGG + chr4_3:59956421-59956440 Msa0652240:CDS 45.0%
GATTCCGTTGCAAAATGCAC+AGG + chr4_3:59956462-59956481 Msa0652240:CDS 45.0%
! TGCACCTGTGCATTTTGCAA+CGG - chr4_3:59956469-59956488 None:intergenic 45.0%
TTGCAAAATGCACAGGTGCA+GGG + chr4_3:59956469-59956488 Msa0652240:CDS 45.0%
TTCCAAACAAACTCCGGATC+AGG - chr4_3:59956613-59956632 None:intergenic 45.0%
CACGCATTCCAAACAAACTC+CGG - chr4_3:59956619-59956638 None:intergenic 45.0%
CGTTAACCGATGGAATCACA+AGG + chr4_3:59955998-59956017 Msa0652240:exon 45.0%
AACCTCGTTTCGCTTCGTAA+CGG - chr4_3:59956228-59956247 None:intergenic 45.0%
GGTTGCAAGAGTAGGATTCT+TGG + chr4_3:59956307-59956326 Msa0652240:CDS 45.0%
GCGAGTATTCCGAATTTACC+TGG + chr4_3:59956397-59956416 Msa0652240:CDS 45.0%
TGGCGGAGGTTGTGTTTATA+CGG + chr4_3:59956417-59956436 Msa0652240:CDS 45.0%
GGAGGTTGTGTTTATACGGT+TGG + chr4_3:59956421-59956440 Msa0652240:CDS 45.0%
GATTCCGTTGCAAAATGCAC+AGG + chr4_3:59956462-59956481 Msa0652240:CDS 45.0%
! TGCACCTGTGCATTTTGCAA+CGG - chr4_3:59956469-59956488 None:intergenic 45.0%
TTGCAAAATGCACAGGTGCA+GGG + chr4_3:59956469-59956488 Msa0652240:CDS 45.0%
TTCCAAACAAACTCCGGATC+AGG - chr4_3:59956613-59956632 None:intergenic 45.0%
CACGCATTCCAAACAAACTC+CGG - chr4_3:59956619-59956638 None:intergenic 45.0%
!! GCCGATTTGAGAGCTGTTCT+TGG - chr4_3:59955946-59955965 None:intergenic 50.0%
CGTAAGCCTAAGCACACGAA+CGG + chr4_3:59956162-59956181 Msa0652240:CDS 50.0%
GTGTATCCGTTCGTGTGCTT+AGG - chr4_3:59956171-59956190 None:intergenic 50.0%
CTCTACGTAGATCCAACCAC+CGG + chr4_3:59956196-59956215 Msa0652240:CDS 50.0%
GTTACAAACGGATCGCGAAC+CGG + chr4_3:59956286-59956305 Msa0652240:CDS 50.0%
ATTCCGAATTTACCTGGCGG+AGG + chr4_3:59956403-59956422 Msa0652240:CDS 50.0%
CAACCTCCGCCAGGTAAATT+CGG - chr4_3:59956409-59956428 None:intergenic 50.0%
GTATAAACACAACCTCCGCC+AGG - chr4_3:59956418-59956437 None:intergenic 50.0%
GTTGCAAAATGCACAGGTGC+AGG + chr4_3:59956468-59956487 Msa0652240:CDS 50.0%
TGCAAAATGCACAGGTGCAG+GGG + chr4_3:59956470-59956489 Msa0652240:CDS 50.0%
TCGTCCGTTTCCGAGTAGAA+TGG + chr4_3:59956576-59956595 Msa0652240:CDS 50.0%
TCCGTTTCCGAGTAGAATGG+CGG + chr4_3:59956579-59956598 Msa0652240:CDS 50.0%
ACCGCCATTCTACTCGGAAA+CGG - chr4_3:59956583-59956602 None:intergenic 50.0%
! AGCCTGATCCGGAGTTTGTT+TGG + chr4_3:59956608-59956627 Msa0652240:CDS 50.0%
! CGGAGTTTGTTTGGAATGCG+TGG + chr4_3:59956617-59956636 Msa0652240:CDS 50.0%
!! GCCGATTTGAGAGCTGTTCT+TGG - chr4_3:59955946-59955965 None:intergenic 50.0%
CGTAAGCCTAAGCACACGAA+CGG + chr4_3:59956162-59956181 Msa0652240:CDS 50.0%
GTGTATCCGTTCGTGTGCTT+AGG - chr4_3:59956171-59956190 None:intergenic 50.0%
CTCTACGTAGATCCAACCAC+CGG + chr4_3:59956196-59956215 Msa0652240:CDS 50.0%
GTTACAAACGGATCGCGAAC+CGG + chr4_3:59956286-59956305 Msa0652240:CDS 50.0%
ATTCCGAATTTACCTGGCGG+AGG + chr4_3:59956403-59956422 Msa0652240:CDS 50.0%
CAACCTCCGCCAGGTAAATT+CGG - chr4_3:59956409-59956428 None:intergenic 50.0%
GTATAAACACAACCTCCGCC+AGG - chr4_3:59956418-59956437 None:intergenic 50.0%
GTTGCAAAATGCACAGGTGC+AGG + chr4_3:59956468-59956487 Msa0652240:CDS 50.0%
TGCAAAATGCACAGGTGCAG+GGG + chr4_3:59956470-59956489 Msa0652240:CDS 50.0%
TCGTCCGTTTCCGAGTAGAA+TGG + chr4_3:59956576-59956595 Msa0652240:CDS 50.0%
TCCGTTTCCGAGTAGAATGG+CGG + chr4_3:59956579-59956598 Msa0652240:CDS 50.0%
ACCGCCATTCTACTCGGAAA+CGG - chr4_3:59956583-59956602 None:intergenic 50.0%
! AGCCTGATCCGGAGTTTGTT+TGG + chr4_3:59956608-59956627 Msa0652240:CDS 50.0%
! CGGAGTTTGTTTGGAATGCG+TGG + chr4_3:59956617-59956636 Msa0652240:CDS 50.0%
TAACGGAGGATTCCGGTGGT+TGG - chr4_3:59956211-59956230 None:intergenic 55.0%
! TTCGTAACGGAGGATTCCGG+TGG - chr4_3:59956215-59956234 None:intergenic 55.0%
! CGCTTCGTAACGGAGGATTC+CGG - chr4_3:59956218-59956237 None:intergenic 55.0%
CTCGTTTCGCTTCGTAACGG+AGG - chr4_3:59956225-59956244 None:intergenic 55.0%
CTCCGTTACGAAGCGAAACG+AGG + chr4_3:59956223-59956242 Msa0652240:CDS 55.0%
ATACGGTTGGGGAGAGTCAG+TGG + chr4_3:59956434-59956453 Msa0652240:CDS 55.0%
AATGCACAGGTGCAGGGGAA+AGG + chr4_3:59956475-59956494 Msa0652240:CDS 55.0%
GGCGGTTAATCAGCCTGATC+CGG + chr4_3:59956597-59956616 Msa0652240:CDS 55.0%
TAACGGAGGATTCCGGTGGT+TGG - chr4_3:59956211-59956230 None:intergenic 55.0%
! TTCGTAACGGAGGATTCCGG+TGG - chr4_3:59956215-59956234 None:intergenic 55.0%
! CGCTTCGTAACGGAGGATTC+CGG - chr4_3:59956218-59956237 None:intergenic 55.0%
CTCGTTTCGCTTCGTAACGG+AGG - chr4_3:59956225-59956244 None:intergenic 55.0%
CTCCGTTACGAAGCGAAACG+AGG + chr4_3:59956223-59956242 Msa0652240:CDS 55.0%
ATACGGTTGGGGAGAGTCAG+TGG + chr4_3:59956434-59956453 Msa0652240:CDS 55.0%
AATGCACAGGTGCAGGGGAA+AGG + chr4_3:59956475-59956494 Msa0652240:CDS 55.0%
GGCGGTTAATCAGCCTGATC+CGG + chr4_3:59956597-59956616 Msa0652240:CDS 55.0%
CGCGAACCGGTTGCAAGAGT+AGG + chr4_3:59956299-59956318 Msa0652240:CDS 60.0%
ACAGGTGCAGGGGAAAGGTG+AGG + chr4_3:59956480-59956499 Msa0652240:CDS 60.0%
CGCGAACCGGTTGCAAGAGT+AGG + chr4_3:59956299-59956318 Msa0652240:CDS 60.0%
ACAGGTGCAGGGGAAAGGTG+AGG + chr4_3:59956480-59956499 Msa0652240:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 59955814 59956752 59955814 ID=Msa0652240;Name=Msa0652240
chr4_3 mRNA 59955814 59956752 59955814 ID=Msa0652240-mRNA-1;Parent=Msa0652240;Name=Msa0652240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=193|1|1|1|1|1|2|47|205
chr4_3 exon 59955814 59956019 59955814 ID=Msa0652240-mRNA-1:exon:16166;Parent=Msa0652240-mRNA-1
chr4_3 exon 59956101 59956752 59956101 ID=Msa0652240-mRNA-1:exon:16167;Parent=Msa0652240-mRNA-1
chr4_3 five_prime_UTR 59955814 59956006 59955814 ID=Msa0652240-mRNA-1:five_prime_utr;Parent=Msa0652240-mRNA-1
chr4_3 CDS 59956007 59956019 59956007 ID=Msa0652240-mRNA-1:cds;Parent=Msa0652240-mRNA-1
chr4_3 CDS 59956101 59956705 59956101 ID=Msa0652240-mRNA-1:cds;Parent=Msa0652240-mRNA-1
chr4_3 three_prime_UTR 59956706 59956752 59956706 ID=Msa0652240-mRNA-1:three_prime_utr;Parent=Msa0652240-mRNA-1
Gene Sequence

>Msa0652240

ATGGAATCACAAGGTGGATTACGAGACACATCCGTTGTAGTCGTAACGCTAGATTCCGACGAAGTATACATAATCGTAAGCCTAAGCACACGAACGGATACACAAATTCTCTACGTAGATCCAACCACCGGAATCCTCCGTTACGAAGCGAAACGAGGTTTCGATCTATTCAATTCACAAAAAGAAGCTTACGAATTCGTTACAAACGGATCGCGAACCGGTTGCAAGAGTAGGATTCTTGGTAGAGCTATATTAGGTTACGCTGCTTTAGGAAATTTCGCTTTTCTTCTAATCGCAACGAGGTTGATTGCGAGTATTCCGAATTTACCTGGCGGAGGTTGTGTTTATACGGTTGGGGAGAGTCAGTGGATTAAGATTCCGTTGCAAAATGCACAGGTGCAGGGGAAAGGTGAGGTGAAGAATGTTATGGAGTTGTTAGAGCTTGACATTGATGGGAAGCATTATTTTTGTGAGACGAGGGATATTACTCGTCCGTTTCCGAGTAGAATGGCGGTTAATCAGCCTGATCCGGAGTTTGTTTGGAATGCGTGGTTTTCTACGCCGTTTGTTAATGTTGGATTGGCTACACATTGTGTTCATCTCTTGCAGGTTTTGTGA
Protein sequence

>Msa0652240

MESQGGLRDTSVVVVTLDSDEVYIIVSLSTRTDTQILYVDPTTGILRYEAKRGFDLFNSQKEAYEFVTNGSRTGCKSRILGRAILGYAALGNFAFLLIATRLIASIPNLPGGGCVYTVGESQWIKIPLQNAQVQGKGEVKNVMELLELDIDGKHYFCETRDITRPFPSRMAVNQPDPEFVWNAWFSTPFVNVGLATHCVHLLQVL*