AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0660440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660440 MtrunA17_Chr8g0387321 94.891 411 5 3 1 411 1 395 0.0 768
Msa0660440 MtrunA17_Chr5g0403251 52.790 233 92 3 190 411 325 550 7.40e-70 234
Msa0660440 MtrunA17_Chr8g0373801 51.807 249 94 4 189 411 354 602 2.57e-67 229
Msa0660440 MtrunA17_Chr1g0155681 47.321 224 88 5 204 411 173 382 1.11e-54 191
Msa0660440 MtrunA17_Chr1g0177751 45.133 226 95 3 201 411 161 372 6.12e-53 187
Msa0660440 MtrunA17_Chr3g0141941 41.949 236 98 6 194 411 157 371 1.08e-49 176
Msa0660440 MtrunA17_Chr7g0267481 44.561 285 134 7 138 411 113 384 2.03e-47 172
Msa0660440 MtrunA17_Chr4g0025521 47.393 211 104 3 205 411 221 428 2.05e-45 167
Msa0660440 MtrunA17_Chr4g0028701 39.130 253 111 7 201 411 245 496 4.17e-39 148
Msa0660440 MtrunA17_Chr4g0008591 39.556 225 88 6 201 411 196 386 7.12e-37 141
Msa0660440 MtrunA17_Chr7g0240371 39.623 212 110 5 201 411 137 331 2.29e-36 139
Msa0660440 MtrunA17_Chr3g0141931 38.914 221 114 7 201 411 165 374 1.44e-35 135
Msa0660440 MtrunA17_Chr2g0327021 38.565 223 120 5 201 411 248 465 3.71e-33 131
Msa0660440 MtrunA17_Chr1g0151681 33.803 142 85 4 270 411 318 450 3.92e-17 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660440 AT5G41410.1 42.487 386 142 11 100 411 38 417 1.10e-72 239
Msa0660440 AT4G36870.4 52.752 218 91 3 201 411 312 524 1.38e-66 221
Msa0660440 AT4G36870.3 52.752 218 91 3 201 411 312 524 3.68e-66 221
Msa0660440 AT2G23760.2 51.152 217 103 1 198 411 234 450 1.58e-65 220
Msa0660440 AT2G23760.1 51.152 217 103 1 198 411 234 450 1.58e-65 220
Msa0660440 AT2G23760.3 51.152 217 103 1 198 411 234 450 1.58e-65 220
Msa0660440 AT4G36870.2 52.752 218 91 3 201 411 312 524 4.09e-65 221
Msa0660440 AT4G36870.1 52.752 218 91 3 201 411 312 524 4.09e-65 221
Msa0660440 AT2G23760.4 51.152 217 103 1 198 411 234 450 1.48e-64 219
Msa0660440 AT1G75410.3 50.000 212 91 5 205 411 171 372 9.95e-56 189
Msa0660440 AT1G19700.1 49.765 213 97 4 205 411 169 377 3.58e-55 191
Msa0660440 AT1G19700.3 49.765 213 97 4 205 411 169 377 3.58e-55 191
Msa0660440 AT1G19700.5 49.765 213 97 4 205 411 169 377 3.58e-55 191
Msa0660440 AT1G19700.4 49.765 213 97 4 205 411 169 377 3.58e-55 191
Msa0660440 AT1G19700.2 49.765 213 97 4 205 411 169 377 3.58e-55 191
Msa0660440 AT1G75410.1 49.761 209 96 4 205 411 171 372 8.52e-55 189
Msa0660440 AT1G75410.2 49.761 209 96 4 205 411 171 372 8.52e-55 189
Msa0660440 AT4G34610.2 44.747 257 125 3 162 411 94 340 1.61e-54 189
Msa0660440 AT4G34610.4 44.747 257 125 3 162 411 94 340 1.61e-54 189
Msa0660440 AT4G34610.3 44.747 257 125 3 162 411 94 340 1.61e-54 189
Msa0660440 AT4G34610.1 44.747 257 125 3 162 411 94 340 1.61e-54 189
Msa0660440 AT2G16400.1 47.867 211 95 4 202 411 115 311 5.52e-52 181
Msa0660440 AT2G35940.3 47.391 230 93 6 202 411 190 411 6.77e-51 182
Msa0660440 AT2G35940.1 47.391 230 93 6 202 411 190 411 6.77e-51 182
Msa0660440 AT2G35940.2 47.391 230 93 6 202 411 190 411 6.77e-51 182
Msa0660440 AT1G75430.1 41.566 166 87 3 255 411 64 228 4.10e-36 134
Msa0660440 AT1G75430.2 41.566 166 87 3 255 411 64 228 7.30e-36 133
Msa0660440 AT2G27220.1 36.098 205 99 4 207 411 82 254 1.73e-32 127
Msa0660440 AT2G27220.3 36.098 205 99 4 207 411 82 254 1.73e-32 127
Msa0660440 AT2G27990.2 36.187 257 129 7 162 411 222 450 9.91e-32 125
Msa0660440 AT5G02030.1 37.727 220 113 5 201 411 170 374 2.57e-31 126
Msa0660440 AT2G27990.1 36.187 257 129 7 162 411 222 450 2.74e-31 125
Msa0660440 AT2G27220.2 33.184 223 99 5 207 411 82 272 9.90e-29 117
Msa0660440 AT4G32980.1 29.384 211 136 4 201 411 201 398 9.69e-18 85.5
Msa0660440 AT4G32980.2 29.384 211 136 4 201 411 201 398 9.69e-18 85.5

Find 72 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGTTCTGTTTCAATTCTC+AGG 0.263185 4_3:-71162501 Msa0660440:CDS
CTGTTTCAATTCTCAGGGCT+TGG 0.264891 4_3:-71162495 Msa0660440:CDS
GTTTCACCCTTTGTTGTTCC+AGG 0.285076 4_3:+71162628 None:intergenic
TGGGTATATGAAGACTAATT+TGG 0.285694 4_3:-71164355 Msa0660440:five_prime_UTR
TCAGCAAACCCTTCCTCTAT+TGG 0.320628 4_3:-71163546 Msa0660440:CDS
TTTGTTGTTCCAGGTGCAAC+AGG 0.331439 4_3:+71162637 None:intergenic
TTGGTTGTATTTATAAATCT+TGG 0.335531 4_3:-71164336 Msa0660440:five_prime_UTR
GTGAATTAATTTATACTATG+AGG 0.354693 4_3:+71164131 None:intergenic
TGCGAAAGTTTATTCAGCTT+TGG 0.371886 4_3:-71162752 Msa0660440:CDS
TTTGAAGCTGTAGCTGGAAA+TGG 0.404194 4_3:-71162778 Msa0660440:CDS
ATAATATTTAGGTTGATAGA+AGG 0.409270 4_3:-71162843 Msa0660440:intron
AGTTCAACAATAAGCAAAGT+TGG 0.410232 4_3:-71163341 Msa0660440:CDS
AAGATGTTGTTGATGTTGTT+TGG 0.412598 4_3:+71163908 None:intergenic
TGAAATCTTGATGTTGTTGT+TGG 0.430842 4_3:+71163751 None:intergenic
GTAGTGAGATTAAACATCTC+TGG 0.433803 4_3:+71163954 None:intergenic
TGAATTAATTTATACTATGA+GGG 0.440235 4_3:+71164132 None:intergenic
TGGATTTGTGTTTGAATCGT+TGG 0.441048 4_3:+71163993 None:intergenic
CTTAACAAGTGATGAGTCAA+TGG 0.447843 4_3:+71164312 None:intergenic
AATCATCATTTACAAGATCA+AGG 0.456108 4_3:-71163492 Msa0660440:CDS
TCATCATTTGAAGCTGTAGC+TGG 0.459401 4_3:-71162784 Msa0660440:CDS
GAGATGTTTAATCTCACTAC+AGG 0.460320 4_3:-71163951 Msa0660440:CDS
AGAAGATGAGATAGCTAGAG+AGG 0.471063 4_3:+71164207 None:intergenic
ATGTGGAAAACAAATTTCTC+AGG 0.472948 4_3:-71163843 Msa0660440:CDS
GTTCAACAATAAGCAAAGTT+GGG 0.473195 4_3:-71163340 Msa0660440:CDS
AAAGAGAGTATATGATAGCA+TGG 0.487912 4_3:-71164246 Msa0660440:five_prime_UTR
AGAATTGAAACAGAACGTTC+AGG 0.492761 4_3:+71162505 None:intergenic
AAAAGATTGTAAACCAATAG+AGG 0.498080 4_3:+71163533 None:intergenic
ACTACAGGGATGGAGATGAT+AGG 0.502219 4_3:-71163936 Msa0660440:CDS
TCATTGAGAAGTTCTTGTGC+TGG 0.509475 4_3:+71163426 None:intergenic
CTGGATCCGACACAAAGCCT+TGG 0.517070 4_3:+71163973 None:intergenic
ACGTTCTGTTTCAATTCTCA+GGG 0.517994 4_3:-71162500 Msa0660440:CDS
TTACAAGATCAAGGGTATCA+AGG 0.518553 4_3:-71163483 Msa0660440:CDS
TCAAACAACAAGAAAAGCAA+TGG 0.524152 4_3:-71162671 Msa0660440:CDS
ACATGATGGAAACACATCCT+TGG 0.528940 4_3:-71162546 Msa0660440:CDS
ATAATATACCTCTTCTAGCA+TGG 0.533662 4_3:+71163218 None:intergenic
GTTGCACCTGGAACAACAAA+GGG 0.538924 4_3:-71162634 Msa0660440:CDS
CAAACAACAAGAAAAGCAAT+GGG 0.541294 4_3:-71162670 Msa0660440:CDS
GATTGTAAACCAATAGAGGA+AGG 0.551563 4_3:+71163537 None:intergenic
AGCACTATTGTAATCAAATG+AGG 0.555931 4_3:-71162816 Msa0660440:CDS
GGTCTTTCATTACCTTCACA+AGG 0.569310 4_3:+71163597 None:intergenic
ATTTCAACAAATCAACATGA+TGG 0.573334 4_3:-71162560 Msa0660440:CDS
TGTTGCACCTGGAACAACAA+AGG 0.574394 4_3:-71162635 Msa0660440:CDS
GAAGATGAGATAGCTAGAGA+GGG 0.581581 4_3:+71164208 None:intergenic
GTTTAATCTCACTACAGGGA+TGG 0.583583 4_3:-71163946 Msa0660440:CDS
GAAGATGACAAAACATCAAG+TGG 0.589082 4_3:-71164032 Msa0660440:CDS
GCTCCTTTCCATGCTAGAAG+AGG 0.590546 4_3:-71163226 Msa0660440:intron
AAACTAAGTCAACAACATGT+TGG 0.592199 4_3:-71163819 Msa0660440:CDS
ATCATCATTTACAAGATCAA+GGG 0.598993 4_3:-71163491 Msa0660440:CDS
ATTGTAAACCAATAGAGGAA+GGG 0.599402 4_3:+71163538 None:intergenic
ATACCTCTTCTAGCATGGAA+AGG 0.599613 4_3:+71163223 None:intergenic
TTGTTGTTCCAGGTGCAACA+GGG 0.600884 4_3:+71162638 None:intergenic
GAGAGTGAAAGTCCTTGACT+TGG 0.606569 4_3:+71163576 None:intergenic
GAATTAATTTATACTATGAG+GGG 0.609485 4_3:+71164133 None:intergenic
ATTCATCATCTTCACATCAG+TGG 0.610423 4_3:-71163656 Msa0660440:CDS
GTCTTTCATTACCTTCACAA+GGG 0.619695 4_3:+71163598 None:intergenic
ACACAACAAACTCTGTCATG+TGG 0.622405 4_3:-71163860 Msa0660440:CDS
AGACCACGTTGAGGTCTCCA+AGG 0.624695 4_3:+71162529 None:intergenic
CAACAAAGGGTGAAACACCT+AGG 0.627180 4_3:-71162621 Msa0660440:CDS
GATGACAAAACATCAAGTGG+TGG 0.632307 4_3:-71164029 Msa0660440:CDS
ACGATTCAAACACAAATCCA+AGG 0.635087 4_3:-71163990 Msa0660440:CDS
TTGTAATCAAATGAGGAGTG+TGG 0.639918 4_3:-71162809 Msa0660440:CDS
TGGGAAGATGATAACAACAA+TGG 0.648007 4_3:-71163321 Msa0660440:CDS
GGTAATGAAAGACCAAGTCA+AGG 0.652296 4_3:-71163588 Msa0660440:CDS
ACAAATCCAAGGCTTTGTGT+CGG 0.652705 4_3:-71163979 Msa0660440:CDS
GAAGATGATAACAACAATGG+TGG 0.654808 4_3:-71163318 Msa0660440:CDS
GGAGCATGAAAAGCATAGTG+TGG 0.654941 4_3:+71164154 None:intergenic
TGATCAGAATCTAAACAACA+TGG 0.698586 4_3:-71163706 Msa0660440:CDS
AGATGTTTAATCTCACTACA+GGG 0.700473 4_3:-71163950 Msa0660440:CDS
CAATTCAACAAACTCTACAG+AGG 0.717090 4_3:+71163266 None:intergenic
CATCCTTGGAGACCTCAACG+TGG 0.740668 4_3:-71162532 Msa0660440:CDS
CGTTCAGGAAGACCACGTTG+AGG 0.743992 4_3:+71162520 None:intergenic
GAGAGTATATGATAGCATGG+AGG 0.763200 4_3:-71164243 Msa0660440:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTGTATAATAATATTT+AGG - chr4_3:71163970-71163989 Msa0660440:CDS 10.0%
!!! TTTTGTGTATAATAATATTT+AGG - chr4_3:71163970-71163989 Msa0660440:CDS 10.0%
!! TGAATTAATTTATACTATGA+GGG + chr4_3:71162695-71162714 None:intergenic 15.0%
!!! AGTTTGAAAATTTTTATTCA+TGG - chr4_3:71162752-71162771 Msa0660440:CDS 15.0%
!! TTATGACTTGAAATAAATTA+AGG + chr4_3:71163704-71163723 None:intergenic 15.0%
!! TGAATTAATTTATACTATGA+GGG + chr4_3:71162695-71162714 None:intergenic 15.0%
!!! AGTTTGAAAATTTTTATTCA+TGG - chr4_3:71162752-71162771 Msa0660440:CDS 15.0%
!! TTATGACTTGAAATAAATTA+AGG + chr4_3:71163704-71163723 None:intergenic 15.0%
!! TTGGTTGTATTTATAAATCT+TGG - chr4_3:71162488-71162507 Msa0660440:CDS 20.0%
!! GAATTAATTTATACTATGAG+GGG + chr4_3:71162694-71162713 None:intergenic 20.0%
!! GTGAATTAATTTATACTATG+AGG + chr4_3:71162696-71162715 None:intergenic 20.0%
!!! ATGATAGAAATTTTCATTCA+TGG + chr4_3:71163051-71163070 None:intergenic 20.0%
!! TTAAAAAGTTTCAATTGTCA+CGG + chr4_3:71163841-71163860 None:intergenic 20.0%
!!! TGAAAACTAACAGTTTTTAT+TGG + chr4_3:71163886-71163905 None:intergenic 20.0%
!!! AGTTTTCATCTTTATTTCTA+TGG - chr4_3:71163898-71163917 Msa0660440:CDS 20.0%
!!! ATAATATTTAGGTTGATAGA+AGG - chr4_3:71163981-71164000 Msa0660440:CDS 20.0%
!! TTGGTTGTATTTATAAATCT+TGG - chr4_3:71162488-71162507 Msa0660440:CDS 20.0%
!! GAATTAATTTATACTATGAG+GGG + chr4_3:71162694-71162713 None:intergenic 20.0%
!! GTGAATTAATTTATACTATG+AGG + chr4_3:71162696-71162715 None:intergenic 20.0%
!!! ATGATAGAAATTTTCATTCA+TGG + chr4_3:71163051-71163070 None:intergenic 20.0%
!! TTAAAAAGTTTCAATTGTCA+CGG + chr4_3:71163841-71163860 None:intergenic 20.0%
!!! TGAAAACTAACAGTTTTTAT+TGG + chr4_3:71163886-71163905 None:intergenic 20.0%
!!! AGTTTTCATCTTTATTTCTA+TGG - chr4_3:71163898-71163917 Msa0660440:CDS 20.0%
!!! ATAATATTTAGGTTGATAGA+AGG - chr4_3:71163981-71164000 Msa0660440:CDS 20.0%
! AAAAGATTGTAAACCAATAG+AGG + chr4_3:71163294-71163313 None:intergenic 25.0%
! AATCATCATTTACAAGATCA+AGG - chr4_3:71163332-71163351 Msa0660440:CDS 25.0%
! ATCATCATTTACAAGATCAA+GGG - chr4_3:71163333-71163352 Msa0660440:CDS 25.0%
!! AATCAAAGACTCTTAACTTT+TGG - chr4_3:71163722-71163741 Msa0660440:CDS 25.0%
! TAAAAAGTTTCAATTGTCAC+GGG + chr4_3:71163840-71163859 None:intergenic 25.0%
! TATTGGTAAGAAAAGGAAAA+AGG + chr4_3:71163869-71163888 None:intergenic 25.0%
!! CAGTTTTTATTGGTAAGAAA+AGG + chr4_3:71163876-71163895 None:intergenic 25.0%
!!! TTTCATCTTTATTTCTATGG+TGG - chr4_3:71163901-71163920 Msa0660440:CDS 25.0%
!!! ATTTTAGGTGTTTGAAAGAT+GGG - chr4_3:71164116-71164135 Msa0660440:five_prime_UTR 25.0%
! ATTTCAACAAATCAACATGA+TGG - chr4_3:71164264-71164283 Msa0660440:five_prime_UTR 25.0%
! AAAAGATTGTAAACCAATAG+AGG + chr4_3:71163294-71163313 None:intergenic 25.0%
! AATCATCATTTACAAGATCA+AGG - chr4_3:71163332-71163351 Msa0660440:CDS 25.0%
! ATCATCATTTACAAGATCAA+GGG - chr4_3:71163333-71163352 Msa0660440:CDS 25.0%
!! AATCAAAGACTCTTAACTTT+TGG - chr4_3:71163722-71163741 Msa0660440:CDS 25.0%
! TAAAAAGTTTCAATTGTCAC+GGG + chr4_3:71163840-71163859 None:intergenic 25.0%
! TATTGGTAAGAAAAGGAAAA+AGG + chr4_3:71163869-71163888 None:intergenic 25.0%
!! CAGTTTTTATTGGTAAGAAA+AGG + chr4_3:71163876-71163895 None:intergenic 25.0%
!!! TTTCATCTTTATTTCTATGG+TGG - chr4_3:71163901-71163920 Msa0660440:CDS 25.0%
!!! ATTTTAGGTGTTTGAAAGAT+GGG - chr4_3:71164116-71164135 Msa0660440:five_prime_UTR 25.0%
! ATTTCAACAAATCAACATGA+TGG - chr4_3:71164264-71164283 Msa0660440:five_prime_UTR 25.0%
TGGGTATATGAAGACTAATT+TGG - chr4_3:71162469-71162488 Msa0660440:CDS 30.0%
! AAAGAGAGTATATGATAGCA+TGG - chr4_3:71162578-71162597 Msa0660440:CDS 30.0%
AGATGTTTAATCTCACTACA+GGG - chr4_3:71162874-71162893 Msa0660440:intron 30.0%
AAGATGTTGTTGATGTTGTT+TGG + chr4_3:71162919-71162938 None:intergenic 30.0%
ATGTGGAAAACAAATTTCTC+AGG - chr4_3:71162981-71163000 Msa0660440:intron 30.0%
AAACTAAGTCAACAACATGT+TGG - chr4_3:71163005-71163024 Msa0660440:intron 30.0%
!! ATGGTTTTTGAAGAAGAAGA+GGG + chr4_3:71163032-71163051 None:intergenic 30.0%
TGAAATCTTGATGTTGTTGT+TGG + chr4_3:71163076-71163095 None:intergenic 30.0%
TGATCAGAATCTAAACAACA+TGG - chr4_3:71163118-71163137 Msa0660440:intron 30.0%
ATTGTAAACCAATAGAGGAA+GGG + chr4_3:71163289-71163308 None:intergenic 30.0%
AGTTCAACAATAAGCAAAGT+TGG - chr4_3:71163483-71163502 Msa0660440:CDS 30.0%
GTTCAACAATAAGCAAAGTT+GGG - chr4_3:71163484-71163503 Msa0660440:CDS 30.0%
ATAATATACCTCTTCTAGCA+TGG + chr4_3:71163609-71163628 None:intergenic 30.0%
! ATGAGATTGGTGTCTTAATT+TGG - chr4_3:71163757-71163776 Msa0660440:CDS 30.0%
AAAAAGTTTCAATTGTCACG+GGG + chr4_3:71163839-71163858 None:intergenic 30.0%
AGCACTATTGTAATCAAATG+AGG - chr4_3:71164008-71164027 Msa0660440:CDS 30.0%
! AAGCTATGTCAAGACATTTT+AGG - chr4_3:71164101-71164120 Msa0660440:five_prime_UTR 30.0%
!! CATTTTAGGTGTTTGAAAGA+TGG - chr4_3:71164115-71164134 Msa0660440:five_prime_UTR 30.0%
TCAAACAACAAGAAAAGCAA+TGG - chr4_3:71164153-71164172 Msa0660440:five_prime_UTR 30.0%
CAAACAACAAGAAAAGCAAT+GGG - chr4_3:71164154-71164173 Msa0660440:five_prime_UTR 30.0%
!! CTATCAAGTATTTTAAGCCT+AGG + chr4_3:71164223-71164242 None:intergenic 30.0%
TGGGTATATGAAGACTAATT+TGG - chr4_3:71162469-71162488 Msa0660440:CDS 30.0%
! AAAGAGAGTATATGATAGCA+TGG - chr4_3:71162578-71162597 Msa0660440:CDS 30.0%
AGATGTTTAATCTCACTACA+GGG - chr4_3:71162874-71162893 Msa0660440:intron 30.0%
AAGATGTTGTTGATGTTGTT+TGG + chr4_3:71162919-71162938 None:intergenic 30.0%
ATGTGGAAAACAAATTTCTC+AGG - chr4_3:71162981-71163000 Msa0660440:intron 30.0%
AAACTAAGTCAACAACATGT+TGG - chr4_3:71163005-71163024 Msa0660440:intron 30.0%
!! ATGGTTTTTGAAGAAGAAGA+GGG + chr4_3:71163032-71163051 None:intergenic 30.0%
TGAAATCTTGATGTTGTTGT+TGG + chr4_3:71163076-71163095 None:intergenic 30.0%
TGATCAGAATCTAAACAACA+TGG - chr4_3:71163118-71163137 Msa0660440:intron 30.0%
ATTGTAAACCAATAGAGGAA+GGG + chr4_3:71163289-71163308 None:intergenic 30.0%
AGTTCAACAATAAGCAAAGT+TGG - chr4_3:71163483-71163502 Msa0660440:CDS 30.0%
GTTCAACAATAAGCAAAGTT+GGG - chr4_3:71163484-71163503 Msa0660440:CDS 30.0%
ATAATATACCTCTTCTAGCA+TGG + chr4_3:71163609-71163628 None:intergenic 30.0%
! ATGAGATTGGTGTCTTAATT+TGG - chr4_3:71163757-71163776 Msa0660440:CDS 30.0%
AAAAAGTTTCAATTGTCACG+GGG + chr4_3:71163839-71163858 None:intergenic 30.0%
AGCACTATTGTAATCAAATG+AGG - chr4_3:71164008-71164027 Msa0660440:CDS 30.0%
! AAGCTATGTCAAGACATTTT+AGG - chr4_3:71164101-71164120 Msa0660440:five_prime_UTR 30.0%
!! CATTTTAGGTGTTTGAAAGA+TGG - chr4_3:71164115-71164134 Msa0660440:five_prime_UTR 30.0%
TCAAACAACAAGAAAAGCAA+TGG - chr4_3:71164153-71164172 Msa0660440:five_prime_UTR 30.0%
CAAACAACAAGAAAAGCAAT+GGG - chr4_3:71164154-71164173 Msa0660440:five_prime_UTR 30.0%
!! CTATCAAGTATTTTAAGCCT+AGG + chr4_3:71164223-71164242 None:intergenic 30.0%
CTTAACAAGTGATGAGTCAA+TGG + chr4_3:71162515-71162534 None:intergenic 35.0%
GAAGATGACAAAACATCAAG+TGG - chr4_3:71162792-71162811 Msa0660440:CDS 35.0%
TGGATTTGTGTTTGAATCGT+TGG + chr4_3:71162834-71162853 None:intergenic 35.0%
ACGATTCAAACACAAATCCA+AGG - chr4_3:71162834-71162853 Msa0660440:CDS 35.0%
GTAGTGAGATTAAACATCTC+TGG + chr4_3:71162873-71162892 None:intergenic 35.0%
GAGATGTTTAATCTCACTAC+AGG - chr4_3:71162873-71162892 Msa0660440:intron 35.0%
!! CATGGTTTTTGAAGAAGAAG+AGG + chr4_3:71163033-71163052 None:intergenic 35.0%
ATTCATCATCTTCACATCAG+TGG - chr4_3:71163168-71163187 Msa0660440:intron 35.0%
GTCTTTCATTACCTTCACAA+GGG + chr4_3:71163229-71163248 None:intergenic 35.0%
GATTGTAAACCAATAGAGGA+AGG + chr4_3:71163290-71163309 None:intergenic 35.0%
!! TTACAAGATCAAGGGTATCA+AGG - chr4_3:71163341-71163360 Msa0660440:CDS 35.0%
TGGGAAGATGATAACAACAA+TGG - chr4_3:71163503-71163522 Msa0660440:CDS 35.0%
GAAGATGATAACAACAATGG+TGG - chr4_3:71163506-71163525 Msa0660440:CDS 35.0%
CAATTCAACAAACTCTACAG+AGG + chr4_3:71163561-71163580 None:intergenic 35.0%
!! GTTGAAGTAGTTGATGAGAT+TGG - chr4_3:71163744-71163763 Msa0660440:CDS 35.0%
TTGTAATCAAATGAGGAGTG+TGG - chr4_3:71164015-71164034 Msa0660440:CDS 35.0%
TGCGAAAGTTTATTCAGCTT+TGG - chr4_3:71164072-71164091 Msa0660440:exon 35.0%
AGAATTGAAACAGAACGTTC+AGG + chr4_3:71164322-71164341 None:intergenic 35.0%
AACGTTCTGTTTCAATTCTC+AGG - chr4_3:71164323-71164342 Msa0660440:five_prime_UTR 35.0%
ACGTTCTGTTTCAATTCTCA+GGG - chr4_3:71164324-71164343 Msa0660440:five_prime_UTR 35.0%
CTTAACAAGTGATGAGTCAA+TGG + chr4_3:71162515-71162534 None:intergenic 35.0%
GAAGATGACAAAACATCAAG+TGG - chr4_3:71162792-71162811 Msa0660440:CDS 35.0%
TGGATTTGTGTTTGAATCGT+TGG + chr4_3:71162834-71162853 None:intergenic 35.0%
ACGATTCAAACACAAATCCA+AGG - chr4_3:71162834-71162853 Msa0660440:CDS 35.0%
GTAGTGAGATTAAACATCTC+TGG + chr4_3:71162873-71162892 None:intergenic 35.0%
GAGATGTTTAATCTCACTAC+AGG - chr4_3:71162873-71162892 Msa0660440:intron 35.0%
!! CATGGTTTTTGAAGAAGAAG+AGG + chr4_3:71163033-71163052 None:intergenic 35.0%
ATTCATCATCTTCACATCAG+TGG - chr4_3:71163168-71163187 Msa0660440:intron 35.0%
GTCTTTCATTACCTTCACAA+GGG + chr4_3:71163229-71163248 None:intergenic 35.0%
GATTGTAAACCAATAGAGGA+AGG + chr4_3:71163290-71163309 None:intergenic 35.0%
!! TTACAAGATCAAGGGTATCA+AGG - chr4_3:71163341-71163360 Msa0660440:CDS 35.0%
TGGGAAGATGATAACAACAA+TGG - chr4_3:71163503-71163522 Msa0660440:CDS 35.0%
GAAGATGATAACAACAATGG+TGG - chr4_3:71163506-71163525 Msa0660440:CDS 35.0%
CAATTCAACAAACTCTACAG+AGG + chr4_3:71163561-71163580 None:intergenic 35.0%
!! GTTGAAGTAGTTGATGAGAT+TGG - chr4_3:71163744-71163763 Msa0660440:CDS 35.0%
TTGTAATCAAATGAGGAGTG+TGG - chr4_3:71164015-71164034 Msa0660440:CDS 35.0%
TGCGAAAGTTTATTCAGCTT+TGG - chr4_3:71164072-71164091 Msa0660440:exon 35.0%
AGAATTGAAACAGAACGTTC+AGG + chr4_3:71164322-71164341 None:intergenic 35.0%
AACGTTCTGTTTCAATTCTC+AGG - chr4_3:71164323-71164342 Msa0660440:five_prime_UTR 35.0%
ACGTTCTGTTTCAATTCTCA+GGG - chr4_3:71164324-71164343 Msa0660440:five_prime_UTR 35.0%
! GAGAGTATATGATAGCATGG+AGG - chr4_3:71162581-71162600 Msa0660440:CDS 40.0%
GAAGATGAGATAGCTAGAGA+GGG + chr4_3:71162619-71162638 None:intergenic 40.0%
AGAAGATGAGATAGCTAGAG+AGG + chr4_3:71162620-71162639 None:intergenic 40.0%
GATGACAAAACATCAAGTGG+TGG - chr4_3:71162795-71162814 Msa0660440:CDS 40.0%
! ACAAATCCAAGGCTTTGTGT+CGG - chr4_3:71162845-71162864 Msa0660440:intron 40.0%
GTTTAATCTCACTACAGGGA+TGG - chr4_3:71162878-71162897 Msa0660440:intron 40.0%
ACACAACAAACTCTGTCATG+TGG - chr4_3:71162964-71162983 Msa0660440:intron 40.0%
! AGATGTGTTTTCCCTTGTGA+AGG - chr4_3:71163215-71163234 Msa0660440:intron 40.0%
GGTCTTTCATTACCTTCACA+AGG + chr4_3:71163230-71163249 None:intergenic 40.0%
GGTAATGAAAGACCAAGTCA+AGG - chr4_3:71163236-71163255 Msa0660440:CDS 40.0%
TCATTGAGAAGTTCTTGTGC+TGG + chr4_3:71163401-71163420 None:intergenic 40.0%
ATACCTCTTCTAGCATGGAA+AGG + chr4_3:71163604-71163623 None:intergenic 40.0%
! TCATCATTTGAAGCTGTAGC+TGG - chr4_3:71164040-71164059 Msa0660440:CDS 40.0%
! TTTGAAGCTGTAGCTGGAAA+TGG - chr4_3:71164046-71164065 Msa0660440:CDS 40.0%
! ACATGATGGAAACACATCCT+TGG - chr4_3:71164278-71164297 Msa0660440:five_prime_UTR 40.0%
! GAGAGTATATGATAGCATGG+AGG - chr4_3:71162581-71162600 Msa0660440:CDS 40.0%
GAAGATGAGATAGCTAGAGA+GGG + chr4_3:71162619-71162638 None:intergenic 40.0%
AGAAGATGAGATAGCTAGAG+AGG + chr4_3:71162620-71162639 None:intergenic 40.0%
GATGACAAAACATCAAGTGG+TGG - chr4_3:71162795-71162814 Msa0660440:CDS 40.0%
! ACAAATCCAAGGCTTTGTGT+CGG - chr4_3:71162845-71162864 Msa0660440:intron 40.0%
GTTTAATCTCACTACAGGGA+TGG - chr4_3:71162878-71162897 Msa0660440:intron 40.0%
ACACAACAAACTCTGTCATG+TGG - chr4_3:71162964-71162983 Msa0660440:intron 40.0%
! AGATGTGTTTTCCCTTGTGA+AGG - chr4_3:71163215-71163234 Msa0660440:intron 40.0%
GGTCTTTCATTACCTTCACA+AGG + chr4_3:71163230-71163249 None:intergenic 40.0%
GGTAATGAAAGACCAAGTCA+AGG - chr4_3:71163236-71163255 Msa0660440:CDS 40.0%
TCATTGAGAAGTTCTTGTGC+TGG + chr4_3:71163401-71163420 None:intergenic 40.0%
ATACCTCTTCTAGCATGGAA+AGG + chr4_3:71163604-71163623 None:intergenic 40.0%
! TCATCATTTGAAGCTGTAGC+TGG - chr4_3:71164040-71164059 Msa0660440:CDS 40.0%
! TTTGAAGCTGTAGCTGGAAA+TGG - chr4_3:71164046-71164065 Msa0660440:CDS 40.0%
! ACATGATGGAAACACATCCT+TGG - chr4_3:71164278-71164297 Msa0660440:five_prime_UTR 40.0%
! GGAGCATGAAAAGCATAGTG+TGG + chr4_3:71162673-71162692 None:intergenic 45.0%
ACTACAGGGATGGAGATGAT+AGG - chr4_3:71162888-71162907 Msa0660440:intron 45.0%
GAGAGTGAAAGTCCTTGACT+TGG + chr4_3:71163251-71163270 None:intergenic 45.0%
TCAGCAAACCCTTCCTCTAT+TGG - chr4_3:71163278-71163297 Msa0660440:CDS 45.0%
TTTCAATTGTCACGGGGTGA+AGG + chr4_3:71163833-71163852 None:intergenic 45.0%
! TTGTTGTTCCAGGTGCAACA+GGG + chr4_3:71164189-71164208 None:intergenic 45.0%
!! TTTGTTGTTCCAGGTGCAAC+AGG + chr4_3:71164190-71164209 None:intergenic 45.0%
TGTTGCACCTGGAACAACAA+AGG - chr4_3:71164189-71164208 Msa0660440:five_prime_UTR 45.0%
GTTGCACCTGGAACAACAAA+GGG - chr4_3:71164190-71164209 Msa0660440:five_prime_UTR 45.0%
!! GTTTCACCCTTTGTTGTTCC+AGG + chr4_3:71164199-71164218 None:intergenic 45.0%
CAACAAAGGGTGAAACACCT+AGG - chr4_3:71164203-71164222 Msa0660440:five_prime_UTR 45.0%
CTGTTTCAATTCTCAGGGCT+TGG - chr4_3:71164329-71164348 Msa0660440:five_prime_UTR 45.0%
! GGAGCATGAAAAGCATAGTG+TGG + chr4_3:71162673-71162692 None:intergenic 45.0%
ACTACAGGGATGGAGATGAT+AGG - chr4_3:71162888-71162907 Msa0660440:intron 45.0%
GAGAGTGAAAGTCCTTGACT+TGG + chr4_3:71163251-71163270 None:intergenic 45.0%
TCAGCAAACCCTTCCTCTAT+TGG - chr4_3:71163278-71163297 Msa0660440:CDS 45.0%
TTTCAATTGTCACGGGGTGA+AGG + chr4_3:71163833-71163852 None:intergenic 45.0%
! TTGTTGTTCCAGGTGCAACA+GGG + chr4_3:71164189-71164208 None:intergenic 45.0%
!! TTTGTTGTTCCAGGTGCAAC+AGG + chr4_3:71164190-71164209 None:intergenic 45.0%
TGTTGCACCTGGAACAACAA+AGG - chr4_3:71164189-71164208 Msa0660440:five_prime_UTR 45.0%
GTTGCACCTGGAACAACAAA+GGG - chr4_3:71164190-71164209 Msa0660440:five_prime_UTR 45.0%
!! GTTTCACCCTTTGTTGTTCC+AGG + chr4_3:71164199-71164218 None:intergenic 45.0%
CAACAAAGGGTGAAACACCT+AGG - chr4_3:71164203-71164222 Msa0660440:five_prime_UTR 45.0%
CTGTTTCAATTCTCAGGGCT+TGG - chr4_3:71164329-71164348 Msa0660440:five_prime_UTR 45.0%
GCTCCTTTCCATGCTAGAAG+AGG - chr4_3:71163598-71163617 Msa0660440:CDS 50.0%
GAAAAAGACCCTGTTGCACC+TGG - chr4_3:71164178-71164197 Msa0660440:five_prime_UTR 50.0%
GCTCCTTTCCATGCTAGAAG+AGG - chr4_3:71163598-71163617 Msa0660440:CDS 50.0%
GAAAAAGACCCTGTTGCACC+TGG - chr4_3:71164178-71164197 Msa0660440:five_prime_UTR 50.0%
CTGGATCCGACACAAAGCCT+TGG + chr4_3:71162854-71162873 None:intergenic 55.0%
CATCCTTGGAGACCTCAACG+TGG - chr4_3:71164292-71164311 Msa0660440:five_prime_UTR 55.0%
AGACCACGTTGAGGTCTCCA+AGG + chr4_3:71164298-71164317 None:intergenic 55.0%
CGTTCAGGAAGACCACGTTG+AGG + chr4_3:71164307-71164326 None:intergenic 55.0%
CTGGATCCGACACAAAGCCT+TGG + chr4_3:71162854-71162873 None:intergenic 55.0%
CATCCTTGGAGACCTCAACG+TGG - chr4_3:71164292-71164311 Msa0660440:five_prime_UTR 55.0%
AGACCACGTTGAGGTCTCCA+AGG + chr4_3:71164298-71164317 None:intergenic 55.0%
CGTTCAGGAAGACCACGTTG+AGG + chr4_3:71164307-71164326 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 71162468 71164378 71162468 ID=Msa0660440;Name=Msa0660440
chr4_3 mRNA 71162468 71164378 71162468 ID=Msa0660440-mRNA-1;Parent=Msa0660440;Name=Msa0660440-mRNA-1;_AED=0.27;_eAED=0.27;_QI=303|1|0.5|1|1|1|2|0|411
chr4_3 exon 71162468 71162854 71162468 ID=Msa0660440-mRNA-1:exon:21294;Parent=Msa0660440-mRNA-1
chr4_3 exon 71163227 71164378 71163227 ID=Msa0660440-mRNA-1:exon:21293;Parent=Msa0660440-mRNA-1
chr4_3 five_prime_UTR 71164076 71164378 71164076 ID=Msa0660440-mRNA-1:five_prime_utr;Parent=Msa0660440-mRNA-1
chr4_3 CDS 71163227 71164075 71163227 ID=Msa0660440-mRNA-1:cds;Parent=Msa0660440-mRNA-1
chr4_3 CDS 71162468 71162854 71162468 ID=Msa0660440-mRNA-1:cds;Parent=Msa0660440-mRNA-1
Gene Sequence

>Msa0660440

ATGGCTAGACAACTTTGTCATGAAGATGACAAAACATCAAGTGGTGGATTTTGTTACAATCCAACGATTCAAACACAAATCCAAGGCTTTGTGTCGGATCCAGAGATGTTTAATCTCACTACAGGGATGGAGATGATAGGTTTTACCAAACAACATCAACAACATCTTCAACAACAACAACAACATAGTACTGACACAACAAACTCTGTCATGTGGAAAACAAATTTCTCAGGAAAACTAAGTCAACAACATGTTGGTCCCTCTTCTTCTTCAAAAACCATGAATGAAAATTTCTATCATCACCAACAACAACATCAAGATTTCACTGTTTCTGATACAACAAGTAGTGATCAGAATCTAAACAACATGGTTCATCATCATCATCATCAACAACATGATTCATCATCTTCACATCAGTGGCAACAAGTTGATGATTCATCTTTAAGATGTGTTTTCCCTTGTGAAGGTAATGAAAGACCAAGTCAAGGACTTTCACTCTCTCTTAGTTCAGCAAACCCTTCCTCTATTGGTTTACAATCTTTTGAACTAAGACACACTACTAATCATCATTTACAAGATCAAGGGTATCAAGGTCATTTTCTTCTCAAAAACTCTAAGTTCTTACTTCCAGCACAAGAACTTCTCAATGAGTTTTGTAGTTTAGAAAGTACAAAGCAAAATGATTTAGTTTTACAAAAGCAAAAATCTCAAAAGTTCAACAATAAGCAAAGTTGGGAAGATGATAACAACAATGGTGGTAGCAGTTCTTCAATCAAACAATCTTTAACCTCTGTAGAGTTTGTTGAATTGCAGAAGAGAAAAACTAAGCTCCTTTCCATGCTAGAAGAGGTTGATAGAAGGTACAAGCACTATTGTAATCAAATGAGGAGTGTGGTTTCATCATTTGAAGCTGTAGCTGGAAATGGAGCTGCGAAAGTTTATTCAGCTTTGGCTTTAAAAGCTATGTCAAGACATTTTAGGTGTTTGAAAGATGGGATTTTAGAACAAATTCAAACAACAAGAAAAGCAATGGGAGAAAAAGACCCTGTTGCACCTGGAACAACAAAGGGTGAAACACCTAGGCTTAAAATACTTGATAGAGTTTTGAGACAACAAAGAGCATTTCAACAAATCAACATGATGGAAACACATCCTTGGAGACCTCAACGTGGTCTTCCTGAACGTTCTGTTTCAATTCTCAGGGCTTGGCTTTTTGAACACTTTCTTCATCCGTAA
Protein sequence

>Msa0660440

MARQLCHEDDKTSSGGFCYNPTIQTQIQGFVSDPEMFNLTTGMEMIGFTKQHQQHLQQQQQHSTDTTNSVMWKTNFSGKLSQQHVGPSSSSKTMNENFYHHQQQHQDFTVSDTTSSDQNLNNMVHHHHHQQHDSSSSHQWQQVDDSSLRCVFPCEGNERPSQGLSLSLSSANPSSIGLQSFELRHTTNHHLQDQGYQGHFLLKNSKFLLPAQELLNEFCSLESTKQNDLVLQKQKSQKFNNKQSWEDDNNNGGSSSSIKQSLTSVEFVELQKRKTKLLSMLEEVDRRYKHYCNQMRSVVSSFEAVAGNGAAKVYSALALKAMSRHFRCLKDGILEQIQTTRKAMGEKDPVAPGTTKGETPRLKILDRVLRQQRAFQQINMMETHPWRPQRGLPERSVSILRAWLFEHFLHP*