AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0679330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0679330 MtrunA17_Chr4g0025401 99.279 416 2 1 1 415 1 416 0.0 847
Msa0679330 MtrunA17_Chr2g0322181 88.517 418 44 2 1 415 1 417 0.0 696
Msa0679330 MtrunA17_Chr8g0334811 70.213 423 118 5 1 415 1 423 0.0 580
Msa0679330 MtrunA17_Chr7g0269841 38.218 348 179 9 14 356 27 343 1.08e-58 195
Msa0679330 MtrunA17_Chr4g0040501 33.053 357 207 10 14 356 5 343 9.68e-49 169
Msa0679330 MtrunA17_Chr2g0282741 33.520 358 199 10 14 356 5 338 3.16e-48 167
Msa0679330 MtrunA17_Chr3g0123861 34.097 349 213 9 14 353 83 423 2.97e-47 167
Msa0679330 MtrunA17_Chr2g0333221 31.921 354 213 10 11 345 68 412 1.20e-46 167
Msa0679330 MtrunA17_Chr2g0324921 32.402 358 199 8 14 356 5 334 6.49e-46 161
Msa0679330 MtrunA17_Chr4g0010781 33.903 351 190 11 14 345 87 414 3.05e-45 162
Msa0679330 MtrunA17_Chr6g0478651 31.768 362 219 11 14 354 69 423 2.02e-44 161
Msa0679330 MtrunA17_Chr2g0282761 34.302 344 198 11 14 344 5 333 5.00e-43 154
Msa0679330 MtrunA17_Chr3g0138471 30.899 356 198 6 14 356 5 325 3.17e-41 148
Msa0679330 MtrunA17_Chr4g0006391 30.905 398 156 12 14 356 5 338 1.23e-40 147
Msa0679330 MtrunA17_Chr3g0087741 30.194 361 210 15 1 345 80 414 7.76e-36 136
Msa0679330 MtrunA17_Chr5g0426951 27.913 369 222 14 1 348 70 415 3.95e-28 115
Msa0679330 MtrunA17_Chr5g0415751 35.172 145 92 1 212 356 18 160 5.55e-27 105
Msa0679330 MtrunA17_Chr3g0093111 26.064 376 177 12 14 356 3 310 9.99e-26 106
Msa0679330 MtrunA17_Chr3g0124801 33.793 145 93 2 213 356 149 291 2.17e-18 85.1
Msa0679330 MtrunA17_Chr2g0277721 31.250 144 98 1 213 356 117 259 2.91e-16 78.6
Msa0679330 MtrunA17_Chr1g0209551 52.941 68 28 1 15 78 18 85 8.45e-14 72.8
Msa0679330 MtrunA17_Chr8g0352451 46.316 95 41 3 12 102 71 159 1.48e-13 72.0
Msa0679330 MtrunA17_Chr5g0399201 41.250 80 40 1 10 82 9 88 3.06e-13 71.2
Msa0679330 MtrunA17_Chr5g0393371 42.105 95 45 3 12 102 5 93 5.95e-13 70.1
Msa0679330 MtrunA17_Chr7g0263971 51.282 78 32 2 4 76 360 436 1.04e-12 69.7
Msa0679330 MtrunA17_Chr1g0159511 42.708 96 43 4 12 102 5 93 3.06e-12 67.8
Msa0679330 MtrunA17_Chr1g0159491 46.377 69 33 2 14 78 7 75 3.43e-12 62.0
Msa0679330 MtrunA17_Chr7g0242951 47.692 65 31 1 15 76 29 93 1.12e-11 66.6
Msa0679330 MtrunA17_Chr2g0307061 37.000 100 50 2 14 100 111 210 1.41e-11 65.5
Msa0679330 MtrunA17_Chr5g0415731 33.333 144 60 4 14 144 6 126 1.44e-11 62.0
Msa0679330 MtrunA17_Chr3g0091361 48.052 77 35 2 11 82 3 79 2.78e-11 62.8
Msa0679330 MtrunA17_Chr7g0271891 40.000 75 37 1 14 80 17 91 4.30e-11 61.2
Msa0679330 MtrunA17_Chr5g0414471 44.928 69 34 1 15 79 27 95 6.69e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0679330 AT5G22060.1 82.143 420 69 4 1 415 1 419 0.0 669
Msa0679330 AT3G44110.1 84.798 421 57 4 1 415 1 420 0.0 655
Msa0679330 AT3G44110.2 87.651 332 37 3 1 329 1 331 0.0 530
Msa0679330 AT3G62600.1 36.648 352 181 11 14 356 27 345 3.93e-55 186
Msa0679330 AT2G20560.1 33.618 351 206 7 14 356 5 336 1.42e-51 176
Msa0679330 AT1G80030.4 30.380 395 246 11 14 388 76 461 4.54e-48 171
Msa0679330 AT1G80030.2 30.380 395 246 11 14 388 76 461 5.72e-48 171
Msa0679330 AT1G80030.3 30.380 395 246 11 14 388 76 461 5.72e-48 171
Msa0679330 AT1G80030.1 30.380 395 246 11 14 388 76 461 5.72e-48 171
Msa0679330 AT5G48030.1 34.375 352 199 13 14 348 95 431 1.28e-46 166
Msa0679330 AT2G22360.1 32.703 370 226 9 14 376 87 440 2.31e-46 165
Msa0679330 AT4G28480.1 30.791 354 223 7 14 356 5 347 3.98e-45 160
Msa0679330 AT5G25530.2 31.856 361 200 10 14 356 5 337 3.66e-44 157
Msa0679330 AT5G01390.1 30.831 373 184 11 14 356 5 333 3.11e-43 154
Msa0679330 AT4G39960.1 31.322 348 218 8 12 345 84 424 1.90e-42 155
Msa0679330 AT4G39960.2 31.322 348 218 8 12 345 84 424 1.90e-42 155
Msa0679330 AT3G08910.1 32.105 380 157 12 14 356 5 320 9.09e-42 150
Msa0679330 AT1G10350.1 30.376 372 200 11 14 356 5 346 3.35e-41 149
Msa0679330 AT1G59725.1 31.054 351 207 9 14 356 5 328 3.48e-41 149
Msa0679330 AT5G25530.1 31.165 369 200 11 14 356 5 345 3.66e-41 149
Msa0679330 AT3G17830.1 29.859 355 219 9 12 345 62 407 7.50e-36 138
Msa0679330 AT1G28210.3 28.117 377 230 12 7 366 42 394 3.96e-34 131
Msa0679330 AT1G28210.2 28.117 377 230 12 7 366 42 394 5.78e-34 131
Msa0679330 AT1G28210.1 28.117 377 230 12 7 366 42 394 6.30e-34 131
Msa0679330 AT2G20550.1 37.931 145 88 1 212 356 141 283 1.07e-26 108
Msa0679330 AT2G20550.2 37.931 145 88 1 212 356 141 283 1.07e-26 108
Msa0679330 AT3G47940.1 29.952 207 128 2 150 356 157 346 4.89e-26 108
Msa0679330 AT3G47940.1 32.990 194 67 3 14 144 5 198 1.92e-20 92.4
Msa0679330 AT4G28480.2 35.374 147 91 2 210 356 147 289 1.47e-23 100
Msa0679330 AT4G28480.2 41.441 111 55 2 14 119 5 110 3.13e-18 85.1
Msa0679330 AT5G01390.2 35.507 138 87 1 219 356 140 275 3.25e-23 99.0
Msa0679330 AT5G01390.2 39.344 122 49 4 14 110 5 126 3.11e-16 79.0
Msa0679330 AT5G01390.4 35.870 184 68 6 14 147 5 188 1.92e-21 92.8
Msa0679330 AT1G11040.1 34.722 144 92 1 213 356 291 432 6.09e-18 85.9
Msa0679330 AT5G01390.3 39.344 122 49 4 14 110 5 126 1.96e-16 79.0
Msa0679330 AT1G44160.1 31.111 135 91 1 222 356 221 353 1.65e-15 77.8
Msa0679330 AT2G21510.5 44.211 95 43 3 12 102 5 93 3.81e-14 73.6
Msa0679330 AT2G21510.2 44.211 95 43 3 12 102 5 93 4.66e-14 73.6
Msa0679330 AT2G21510.4 44.211 95 43 3 12 102 5 93 4.66e-14 73.6
Msa0679330 AT2G21510.3 44.211 95 43 3 12 102 5 93 5.00e-14 73.2
Msa0679330 AT2G21510.1 44.211 95 43 3 12 102 5 93 5.00e-14 73.2
Msa0679330 AT1G77020.1 44.792 96 41 4 12 102 5 93 1.41e-13 72.4
Msa0679330 AT1G59980.2 47.059 68 32 1 15 78 25 92 3.97e-13 70.9
Msa0679330 AT3G08970.1 46.914 81 40 1 8 85 22 102 4.55e-13 71.2
Msa0679330 AT3G08970.2 46.914 81 40 1 8 85 22 102 4.55e-13 71.2
Msa0679330 AT5G03160.1 52.632 95 37 4 4 93 361 452 4.56e-13 71.2
Msa0679330 AT1G59980.1 47.059 68 32 1 15 78 25 92 5.86e-13 70.5
Msa0679330 AT5G05750.1 40.777 103 48 4 14 103 115 217 1.04e-12 68.9
Msa0679330 AT4G39150.3 42.553 94 44 3 12 101 5 92 1.33e-12 68.9
Msa0679330 AT4G39150.1 42.553 94 44 3 12 101 5 92 1.33e-12 68.9
Msa0679330 AT4G39150.2 42.553 94 44 3 12 101 5 92 1.33e-12 68.9
Msa0679330 AT1G21080.3 41.053 95 45 3 12 102 5 92 2.62e-12 68.6
Msa0679330 AT1G21080.1 41.053 95 45 3 12 102 5 92 2.99e-12 68.2
Msa0679330 AT1G68370.1 51.471 68 29 1 15 78 19 86 3.43e-12 68.2
Msa0679330 AT3G57340.1 39.394 99 46 3 14 98 114 212 5.57e-12 67.4
Msa0679330 AT3G57340.2 39.394 99 46 3 14 98 114 212 5.57e-12 67.4
Msa0679330 AT2G35720.1 38.824 85 45 1 5 82 7 91 7.89e-11 64.3
Msa0679330 AT1G76700.1 41.053 95 46 3 12 102 5 93 8.69e-11 63.9
Msa0679330 AT1G24120.1 45.588 68 33 2 15 78 22 89 8.73e-11 63.9

Find 154 sgRNAs with CRISPR-Local

Find 311 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGAAATACTTAATATGTTT+TGG 0.247598 4_4:-36946596 Msa0679330:intron
GACATCTTCTCGTCTTTCTT+TGG 0.252245 4_4:-36949250 Msa0679330:intron
TACAGCAAAGGTTCTAAATC+TGG 0.260581 4_4:-36947873 Msa0679330:intron
TCATCCTCTCAAGGTCTCTT+TGG 0.272658 4_4:-36949081 Msa0679330:CDS
TGCAAGGATGCTGCATTTGC+TGG 0.288553 4_4:+36947775 None:intergenic
TGGGATTGTGTTTGAGCTTT+GGG 0.291118 4_4:+36950682 None:intergenic
TAAAGCTTGCCCTTCATAAA+TGG 0.299708 4_4:+36947027 None:intergenic
CTGTACAATTAAAATCCTTA+TGG 0.300679 4_4:+36946422 None:intergenic
CCAAAACATATTAAGTATTT+CGG 0.305603 4_4:+36946596 None:intergenic
CTGGGATTGTGTTTGAGCTT+TGG 0.305895 4_4:+36950681 None:intergenic
CCAAGTGAGTAAGCACAAAT+TGG 0.314955 4_4:+36947381 None:intergenic
CTTCTTGTTCTTGAACTACT+AGG 0.315780 4_4:-36950552 Msa0679330:five_prime_UTR
CTATGTAGTGTTTGGTTCAA+TGG 0.319946 4_4:-36946687 Msa0679330:three_prime_UTR
TTGACAACCAGCACATTTCA+TGG 0.323834 4_4:+36947845 None:intergenic
GGAATGGGTGGTGGAGGCGG+TGG 0.324217 4_4:-36949289 Msa0679330:CDS
ATGGGTGGTGGAGGCGGTGG+TGG 0.330271 4_4:-36949286 Msa0679330:CDS
TCGGGATCACCACCCTTATC+AGG 0.339085 4_4:+36950374 None:intergenic
CCAATTTGTGCTTACTCACT+TGG 0.343644 4_4:-36947381 Msa0679330:CDS
TTGCAGCCAGACACAGTTAC+TGG 0.349899 4_4:-36947512 Msa0679330:intron
TGATCAGGTGAAAGGTTTGC+AGG 0.350853 4_4:-36946965 Msa0679330:CDS
TACACTTCATGATGTCAATA+TGG 0.351289 4_4:-36946878 Msa0679330:CDS
GAAGGAATGGGTGGTGGAGG+CGG 0.352689 4_4:-36949292 Msa0679330:CDS
CTGAACAAGGATAAGATGTT+TGG 0.368108 4_4:-36950512 Msa0679330:exon
CTCTTGAACAGAAAAGGTTA+AGG 0.385089 4_4:+36946378 None:intergenic
ACGAGGTACTATGAAATCTT+AGG 0.390855 4_4:-36950464 Msa0679330:CDS
ATATGGTGAGGATGCTCTTA+AGG 0.394161 4_4:-36949314 Msa0679330:CDS
ACACGCGAAGTTTGTTATAG+TGG 0.400056 4_4:+36950625 None:intergenic
GACAGAACCTATGTAGTGTT+TGG 0.400741 4_4:-36946695 Msa0679330:three_prime_UTR
TGCATTTGCTGGATCATTGA+TGG 0.402426 4_4:+36947786 None:intergenic
CTTCTCTTCTCTCATTCATT+CGG 0.405261 4_4:-36950591 Msa0679330:five_prime_UTR
CTTCTTATATTTATTGTCGA+TGG 0.406138 4_4:-36946639 Msa0679330:three_prime_UTR
TCAATATGGAAGAGGAGAAT+AGG 0.410593 4_4:-36946864 Msa0679330:CDS
TGAACAGGTGGTGGTGGTGG+TGG 0.412145 4_4:-36949146 Msa0679330:intron
TCTTGAACAGAAAAGGTTAA+GGG 0.418167 4_4:+36946379 None:intergenic
TCCTCTCAAGGTCTCTTTGG+AGG 0.418220 4_4:-36949078 Msa0679330:CDS
AAAGACGAGAAGATGTCAAA+TGG 0.431223 4_4:+36949256 None:intergenic
GCATCCTTGCAATGAATGTA+AGG 0.434783 4_4:-36947763 Msa0679330:CDS
AGAGCTAGCACAAGCTTATG+AGG 0.435011 4_4:-36949374 Msa0679330:CDS
TATTAAGAATCATCCTGATA+AGG 0.441385 4_4:-36950387 Msa0679330:CDS
ACAGTGTGTGCTCTACAAAA+AGG 0.442437 4_4:+36947429 None:intergenic
TGATCAAGACTTAGAGTGTC+GGG 0.443069 4_4:+36946982 None:intergenic
TTCTTATATTTATTGTCGAT+GGG 0.446404 4_4:-36946638 Msa0679330:three_prime_UTR
ATAGGTTCTGTCCAACACTA+TGG 0.452002 4_4:+36946706 None:intergenic
ACAGCAAAGGTTCTAAATCT+GGG 0.455064 4_4:-36947872 Msa0679330:intron
GCGAAGTTTGTTATAGTGGT+TGG 0.460448 4_4:+36950629 None:intergenic
ATAAATGGCCTCTGGTACAT+CGG 0.461071 4_4:+36947042 None:intergenic
GCTTTGGGTCCCACGGAAGA+AGG 0.462719 4_4:+36950697 None:intergenic
ATATCCCCAGTAACTGTGTC+TGG 0.462941 4_4:+36947506 None:intergenic
TTCTTGTTCTTGAACTACTA+GGG 0.467185 4_4:-36950551 Msa0679330:five_prime_UTR
ACCAGGCTTGACAACTTCTC+CGG 0.467557 4_4:+36947328 None:intergenic
GAGGAAACAGCAGCAACAAC+AGG 0.467731 4_4:-36946842 Msa0679330:CDS
CACGTCTCTGCCTCCGTCCT+CGG 0.470973 4_4:+36949117 None:intergenic
AATTGTGATGAAGGTTTAGC+TGG 0.472131 4_4:+36946934 None:intergenic
TCATAAATATCACGCTTCTC+AGG 0.473513 4_4:+36949340 None:intergenic
AAAAGAAAACAAACCTTCTC+GGG 0.479638 4_4:+36950356 None:intergenic
ATGTCTGTCAATTGTGATGA+AGG 0.480120 4_4:+36946925 None:intergenic
ATTAAGAATCATCCTGATAA+GGG 0.483087 4_4:-36950386 Msa0679330:CDS
TGCAGCCAGACACAGTTACT+GGG 0.483778 4_4:-36947511 Msa0679330:intron
GTATGAACAGGTGGTGGTGG+TGG 0.487493 4_4:-36949149 Msa0679330:intron
CGTGATATTTATGATACATA+TGG 0.487702 4_4:-36949331 Msa0679330:CDS
TGCCCTTCATAAATGGCCTC+TGG 0.489076 4_4:+36947034 None:intergenic
CTCTCAACATACTGCTTCAT+CGG 0.492790 4_4:+36947602 None:intergenic
TGTACCCTCTGTGCCCCACC+AGG 0.495428 4_4:+36946793 None:intergenic
TCTTTGGAGGATCTTTACCT+TGG 0.498256 4_4:-36949065 Msa0679330:CDS
CGACACTCTAAGTCTTGATC+AGG 0.498606 4_4:-36946980 Msa0679330:CDS
AAGGAAGGAATGGGTGGTGG+AGG 0.501111 4_4:-36949295 Msa0679330:CDS
GCATTTGCTGGATCATTGAT+GGG 0.503185 4_4:+36947787 None:intergenic
GTACCAGAGGCCATTTATGA+AGG 0.504857 4_4:-36947037 Msa0679330:CDS
TTTATCATTGATAGTTTCAC+CGG 0.508139 4_4:+36947737 None:intergenic
CCAGGCTTGACAACTTCTCC+GGG 0.508832 4_4:+36947329 None:intergenic
CTTCTAGACCCTTCTTCCGT+GGG 0.509635 4_4:-36950706 Msa0679330:five_prime_UTR
TAATTAAGAGTGAGATGGAA+TGG 0.509704 4_4:+36950654 None:intergenic
GGTTCGATTTGATAAGAAGT+TGG 0.509723 4_4:+36947351 None:intergenic
CCCGGAGAAGTTGTCAAGCC+TGG 0.510330 4_4:-36947329 Msa0679330:intron
CTTGAAGTTCATGTCGAGAA+AGG 0.510906 4_4:-36947663 Msa0679330:CDS
CTTAAGGAAGGAATGGGTGG+TGG 0.513608 4_4:-36949298 Msa0679330:CDS
AGTGAGATGGAATGGATGGC+TGG 0.513782 4_4:+36950662 None:intergenic
GGGGCTTCCATGAAATGTGC+TGG 0.516637 4_4:-36947852 Msa0679330:CDS
TCTTCTAGACCCTTCTTCCG+TGG 0.517410 4_4:-36950707 Msa0679330:five_prime_UTR
TTGTCAAGGAACTGGTATGA+AGG 0.522018 4_4:-36947829 Msa0679330:CDS
GGATGCTCTTAAGGAAGGAA+TGG 0.523255 4_4:-36949305 Msa0679330:CDS
TGTGCTGGTTGTCAAGGAAC+TGG 0.527018 4_4:-36947837 Msa0679330:CDS
AAGTATTTCGGATGTCAGAA+TGG 0.529360 4_4:+36946608 None:intergenic
CTTGTATGAACAGGTGGTGG+TGG 0.530748 4_4:-36949152 Msa0679330:intron
TGCAATGAATGTAAGGGTAC+CGG 0.531405 4_4:-36947756 Msa0679330:CDS
ACTGCTTCATCGGCTTCACC+AGG 0.531944 4_4:+36947612 None:intergenic
CTAAGTCTTGATCAGGTGAA+AGG 0.533754 4_4:-36946973 Msa0679330:CDS
AGACCGCTGTCCACAGTGCA+AGG 0.534307 4_4:-36947715 Msa0679330:CDS
TGGATTAATTAAGAGTGAGA+TGG 0.534996 4_4:+36950649 None:intergenic
AAGACGAGAAGATGTCAAAT+GGG 0.536873 4_4:+36949257 None:intergenic
GTCTAGAAGAGTGGGAAGTA+CGG 0.543871 4_4:+36950720 None:intergenic
AAGGTTTCTATTAGACACCT+TGG 0.547621 4_4:-36947810 Msa0679330:CDS
CATTGAACCAAACACTACAT+AGG 0.548900 4_4:+36946688 None:intergenic
GATGCTCTTAAGGAAGGAAT+GGG 0.551678 4_4:-36949304 Msa0679330:CDS
CTTTGGGTCCCACGGAAGAA+GGG 0.555485 4_4:+36950698 None:intergenic
GAAGAAGGGTCTAGAAGAGT+GGG 0.556996 4_4:+36950712 None:intergenic
AGTCAGAAGATTACATTCCC+TGG 0.557761 4_4:-36947630 Msa0679330:CDS
TTTGTGCTTACTCACTTGGA+TGG 0.559690 4_4:-36947377 Msa0679330:CDS
TCATGATGTCAATATGGAAG+AGG 0.561923 4_4:-36946872 Msa0679330:CDS
ATATGGAAGAGGAGAATAGG+AGG 0.568120 4_4:-36946861 Msa0679330:CDS
GTGAGATGGAATGGATGGCT+GGG 0.569750 4_4:+36950663 None:intergenic
TCCACAGTGCAAGGGAGAGA+AGG 0.570484 4_4:-36947706 Msa0679330:CDS
AAAGCTTCTTAGATGTTCCA+AGG 0.572913 4_4:+36949048 None:intergenic
GGTGAGGATGCTCTTAAGGA+AGG 0.584094 4_4:-36949310 Msa0679330:CDS
CAGCAAAGGTTCTAAATCTG+GGG 0.584886 4_4:-36947871 Msa0679330:CDS
TGTTTGAGCTTTGGGTCCCA+CGG 0.585107 4_4:+36950690 None:intergenic
GGAAGAAGGGTCTAGAAGAG+TGG 0.586941 4_4:+36950711 None:intergenic
AGATGTGGTTCATCCTCTCA+AGG 0.587250 4_4:-36949090 Msa0679330:CDS
TCTCTTACTGAGGCTTTGTG+TGG 0.587623 4_4:-36947407 Msa0679330:CDS
GCTGGATCATTGATGGGCCA+AGG 0.587884 4_4:+36947793 None:intergenic
TATACATGCAGATTCATACA+AGG 0.587937 4_4:-36947085 Msa0679330:intron
TATGCCTGGTGGGGCACAGA+GGG 0.589588 4_4:-36946797 Msa0679330:CDS
TACAAGGCTATTAACGATGA+GGG 0.590494 4_4:-36947069 Msa0679330:CDS
GTGGTGGTAGTAGCCGAGGA+CGG 0.590849 4_4:-36949130 Msa0679330:CDS
AGAAGATGTCAAATGGGTCA+TGG 0.591968 4_4:+36949263 None:intergenic
ATGAAATGTGCTGGTTGTCA+AGG 0.596299 4_4:-36947843 Msa0679330:CDS
TAAGAGTGAGATGGAATGGA+TGG 0.600894 4_4:+36950658 None:intergenic
GACGAGGATGATGATATGCC+TGG 0.603076 4_4:-36946811 Msa0679330:CDS
GGTGGTGGTGGTAGTAGCCG+AGG 0.603379 4_4:-36949134 Msa0679330:CDS
GCAGAGACGTGGAGAAGATG+TGG 0.607136 4_4:-36949105 Msa0679330:CDS
CATGGAGATCGATGAGTGCG+AGG 0.609688 4_4:-36946905 Msa0679330:CDS
TACCAGAGGCCATTTATGAA+GGG 0.613207 4_4:-36947036 Msa0679330:CDS
CTGATCAAGACTTAGAGTGT+CGG 0.613726 4_4:+36946981 None:intergenic
CGAGGACGGAGGCAGAGACG+TGG 0.614603 4_4:-36949116 Msa0679330:CDS
GACCGCTGTCCACAGTGCAA+GGG 0.616803 4_4:-36947714 Msa0679330:CDS
TTCATCACAATTGACAGACA+TGG 0.617149 4_4:-36946923 Msa0679330:CDS
CTCCCTTGCACTGTGGACAG+CGG 0.620041 4_4:+36947712 None:intergenic
GCTCTTAAGGAAGGAATGGG+TGG 0.621909 4_4:-36949301 Msa0679330:CDS
GTACCCTTACATTCATTGCA+AGG 0.622171 4_4:+36947759 None:intergenic
GCACACACTGTCTCTTACTG+AGG 0.631481 4_4:-36947417 Msa0679330:CDS
AGGATGATGATATGCCTGGT+GGG 0.636687 4_4:-36946807 Msa0679330:CDS
CATCCTTGCAATGAATGTAA+GGG 0.637972 4_4:-36947762 Msa0679330:CDS
TAAGGGTGGTGATCCCGAGA+AGG 0.642218 4_4:-36950369 Msa0679330:intron
TCTTGTGCTCAAAGTGCAGT+GGG 0.652317 4_4:-36949010 Msa0679330:intron
ATACAAGGCTATTAACGATG+AGG 0.654168 4_4:-36947070 Msa0679330:CDS
CTAATACAAAATGTTGACAA+AGG 0.659134 4_4:-36946492 Msa0679330:intron
AGGGAATGCCGATGTACCAG+AGG 0.659833 4_4:-36947050 Msa0679330:CDS
GTGGTAGTAGCCGAGGACGG+AGG 0.663999 4_4:-36949127 Msa0679330:CDS
GTCTTGTGCTCAAAGTGCAG+TGG 0.666176 4_4:-36949011 Msa0679330:CDS
GCAGCCAGACACAGTTACTG+GGG 0.674307 4_4:-36947510 Msa0679330:intron
GGATGATGATATGCCTGGTG+GGG 0.675094 4_4:-36946806 Msa0679330:CDS
CTGCTTCATCGGCTTCACCA+GGG 0.675980 4_4:+36947613 None:intergenic
AAGAATCATCCTGATAAGGG+TGG 0.677133 4_4:-36950383 Msa0679330:CDS
CTTCTTATCAAATCGAACCC+CGG 0.678657 4_4:-36947347 Msa0679330:CDS
ACCTTCTCTCCCTTGCACTG+TGG 0.679545 4_4:+36947705 None:intergenic
TCCTCCAAAGAGACCTTGAG+AGG 0.679609 4_4:+36949077 None:intergenic
CGAAGAAAAGTGATAGTACG+AGG 0.681812 4_4:-36950481 Msa0679330:CDS
GCTTTACATTCACTTCACTG+TGG 0.688048 4_4:-36947010 Msa0679330:CDS
GATCAAGACTTAGAGTGTCG+GGG 0.692079 4_4:+36946983 None:intergenic
ATATGCCTGGTGGGGCACAG+AGG 0.713014 4_4:-36946798 Msa0679330:CDS
GAGGATGATGATATGCCTGG+TGG 0.714325 4_4:-36946808 Msa0679330:CDS
TATTTATGATACATATGGTG+AGG 0.723619 4_4:-36949326 Msa0679330:CDS
CAGGCTTGACAACTTCTCCG+GGG 0.727309 4_4:+36947330 None:intergenic
ACAACAGGAAGCATATGACG+AGG 0.749856 4_4:-36946827 Msa0679330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTAATTTTTTTTTTTTG+TGG - chr4_4:36947657-36947676 Msa0679330:CDS 10.0%
!!! GTTTAATTTTTTTTTTTTGT+GGG - chr4_4:36947658-36947677 Msa0679330:CDS 10.0%
!!! ATTATAATCAAGATTTTTTT+TGG - chr4_4:36949108-36949127 Msa0679330:CDS 10.0%
!!! TTTGAGTTTGATTTAAATTT+TGG - chr4_4:36946836-36946855 Msa0679330:CDS 15.0%
!!! TATATTATACTAGAAAGTTT+TGG - chr4_4:36946864-36946883 Msa0679330:CDS 15.0%
!!! TATATATGATAATGTATCTA+TGG + chr4_4:36948352-36948371 None:intergenic 15.0%
!! AATAATATTGAAAATCAGAT+GGG + chr4_4:36948591-36948610 None:intergenic 15.0%
!! TAATAATATTGAAAATCAGA+TGG + chr4_4:36948592-36948611 None:intergenic 15.0%
!!! TCTGATTTTCAATATTATTA+TGG - chr4_4:36948592-36948611 Msa0679330:intron 15.0%
!!! AAAAGATTTGTAATTTTTTG+TGG - chr4_4:36949955-36949974 Msa0679330:intron 15.0%
!! TTGATTAATTGTTATGTGTT+TGG - chr4_4:36946764-36946783 Msa0679330:exon 20.0%
!!! AGGATTTGTAGAATTTTTTT+AGG - chr4_4:36946909-36946928 Msa0679330:CDS 20.0%
!! TCACAAATTATCAAAAGAAA+AGG + chr4_4:36946954-36946973 None:intergenic 20.0%
!!! TATCTTTTGTTATAGACATA+GGG + chr4_4:36947373-36947392 None:intergenic 20.0%
!!! TTATCTTTTGTTATAGACAT+AGG + chr4_4:36947374-36947393 None:intergenic 20.0%
!! CTTTCATATGCTATATATAT+TGG - chr4_4:36947436-36947455 Msa0679330:CDS 20.0%
!! TCTAATAAGTCAATCATAAA+AGG - chr4_4:36947573-36947592 Msa0679330:intron 20.0%
!!! TTTTTCCAGTGAATTTTTTA+TGG - chr4_4:36948144-36948163 Msa0679330:intron 20.0%
!! TTACAAACAAAAAGACATTT+GGG + chr4_4:36948235-36948254 None:intergenic 20.0%
!! TTTACAAACAAAAAGACATT+TGG + chr4_4:36948236-36948255 None:intergenic 20.0%
!!! TTTTTGTTTGTAAACACATT+TGG - chr4_4:36948242-36948261 Msa0679330:intron 20.0%
!! TCATATATCAAGTGTAAATT+TGG + chr4_4:36948560-36948579 None:intergenic 20.0%
!! ATATAGTAGATTTGTGTAAT+TGG - chr4_4:36948841-36948860 Msa0679330:intron 20.0%
!! TATAGTAGATTTGTGTAATT+GGG - chr4_4:36948842-36948861 Msa0679330:intron 20.0%
!!! TCTTCAGTTTGTTAAATTTT+GGG + chr4_4:36948978-36948997 None:intergenic 20.0%
!!! TAATCAAGATTTTTTTTGGT+TGG - chr4_4:36949112-36949131 Msa0679330:CDS 20.0%
!! TTTGTAATTATTTACAGCAA+AGG - chr4_4:36949182-36949201 Msa0679330:intron 20.0%
!! TTCTTATATTTATTGTCGAT+GGG - chr4_4:36950429-36950448 Msa0679330:CDS 20.0%
!! CCAAAACATATTAAGTATTT+CGG + chr4_4:36950474-36950493 None:intergenic 20.0%
!!! TTTTTGAAACATCTATATCT+TGG - chr4_4:36950496-36950515 Msa0679330:CDS 20.0%
! TATTAAGAATCATCCTGATA+AGG - chr4_4:36946680-36946699 Msa0679330:three_prime_UTR 25.0%
! ATTAAGAATCATCCTGATAA+GGG - chr4_4:36946681-36946700 Msa0679330:three_prime_UTR 25.0%
! AAAAAGAAAACAAACCTTCT+CGG + chr4_4:36946715-36946734 None:intergenic 25.0%
!!! AAAGTTTTGGTGATTGATTT+AGG - chr4_4:36946877-36946896 Msa0679330:CDS 25.0%
!!! AAGTTTTGGTGATTGATTTA+GGG - chr4_4:36946878-36946897 Msa0679330:CDS 25.0%
! TGATTGTTGAGAAATTTGAT+TGG - chr4_4:36946971-36946990 Msa0679330:CDS 25.0%
! CAAAAATAAGACAATCCAAA+CGG + chr4_4:36947158-36947177 None:intergenic 25.0%
!!! ATTTTTGAGCTTATGCAAAA+CGG - chr4_4:36947171-36947190 Msa0679330:intron 25.0%
!!! GACATATTTTAAGTAGGTTT+TGG + chr4_4:36947480-36947499 None:intergenic 25.0%
!!! GCTTATGACATATTTTAAGT+AGG + chr4_4:36947486-36947505 None:intergenic 25.0%
!! TTTACGCGTTTTTATTGAAT+TGG - chr4_4:36947626-36947645 Msa0679330:CDS 25.0%
! CGTGATATTTATGATACATA+TGG - chr4_4:36947736-36947755 Msa0679330:CDS 25.0%
! TATTTATGATACATATGGTG+AGG - chr4_4:36947741-36947760 Msa0679330:CDS 25.0%
!! AGAATTCCTATTGTGTTATT+TGG - chr4_4:36947842-36947861 Msa0679330:CDS 25.0%
! TGTATGGTTTGATTATCTAA+AGG - chr4_4:36947884-36947903 Msa0679330:intron 25.0%
! GTATGGTTTGATTATCTAAA+GGG - chr4_4:36947885-36947904 Msa0679330:intron 25.0%
! TTCATCCATAAAAAATTCAC+TGG + chr4_4:36948152-36948171 None:intergenic 25.0%
!!! TAAACAATCATTTTTCATCC+TGG - chr4_4:36948176-36948195 Msa0679330:intron 25.0%
! GATAATGTATCTATGGAATT+GGG + chr4_4:36948345-36948364 None:intergenic 25.0%
!! TGATAATGTATCTATGGAAT+TGG + chr4_4:36948346-36948365 None:intergenic 25.0%
! AAAACCATGAAAAATAGAAG+AGG + chr4_4:36948624-36948643 None:intergenic 25.0%
!!! TTTTTGCACTGATTTTGTTT+TGG - chr4_4:36948690-36948709 Msa0679330:intron 25.0%
!! TTGTTTTCTTGTTGAAGATT+CGG - chr4_4:36948732-36948751 Msa0679330:intron 25.0%
! AAACATAAGAAAATGTGAGA+TGG + chr4_4:36948937-36948956 None:intergenic 25.0%
!!! GTCTTCAGTTTGTTAAATTT+TGG + chr4_4:36948979-36948998 None:intergenic 25.0%
!! TGTGTATTTTGTCAGTAATT+TGG + chr4_4:36949013-36949032 None:intergenic 25.0%
! TACTGACAAAATACACATTT+AGG - chr4_4:36949016-36949035 Msa0679330:CDS 25.0%
! ATAGCAACTAATGTAAGTAA+TGG + chr4_4:36949163-36949182 None:intergenic 25.0%
!! TTTATCATTGATAGTTTCAC+CGG + chr4_4:36949333-36949352 None:intergenic 25.0%
!!! GTTGAGAGTTTTAGTAATAT+TGG - chr4_4:36949480-36949499 Msa0679330:intron 25.0%
! AGTAGAATCAGAAAAAATGA+TGG - chr4_4:36950308-36950327 Msa0679330:intron 25.0%
! CTTCTTATATTTATTGTCGA+TGG - chr4_4:36950428-36950447 Msa0679330:CDS 25.0%
!! CCGAAATACTTAATATGTTT+TGG - chr4_4:36950471-36950490 Msa0679330:CDS 25.0%
! CTAATACAAAATGTTGACAA+AGG - chr4_4:36950575-36950594 Msa0679330:five_prime_UTR 25.0%
!! TTTTGCTAAGAAAAACCATA+AGG - chr4_4:36950630-36950649 Msa0679330:five_prime_UTR 25.0%
! CTGTACAATTAAAATCCTTA+TGG + chr4_4:36950648-36950667 None:intergenic 25.0%
TAATTAAGAGTGAGATGGAA+TGG + chr4_4:36946416-36946435 None:intergenic 30.0%
TGGATTAATTAAGAGTGAGA+TGG + chr4_4:36946421-36946440 None:intergenic 30.0%
TTCTTGTTCTTGAACTACTA+GGG - chr4_4:36946516-36946535 Msa0679330:intron 30.0%
AAAAGAAAACAAACCTTCTC+GGG + chr4_4:36946714-36946733 None:intergenic 30.0%
!!! GAATTTTTTTAGGTGTAGTG+TGG - chr4_4:36946919-36946938 Msa0679330:CDS 30.0%
CATGAAGGAAAACAAAAACA+AGG + chr4_4:36947017-36947036 None:intergenic 30.0%
!!! CTTGTTTTTGTTTTCCTTCA+TGG - chr4_4:36947015-36947034 Msa0679330:CDS 30.0%
AGTCAATCATAAAAGGTTCA+TGG - chr4_4:36947580-36947599 Msa0679330:intron 30.0%
!! TCCTATTGTGTTATTTGGTT+CGG - chr4_4:36947847-36947866 Msa0679330:CDS 30.0%
AAAACCCAATTCCATTTGAA+GGG + chr4_4:36948512-36948531 None:intergenic 30.0%
AAAAACCCAATTCCATTTGA+AGG + chr4_4:36948513-36948532 None:intergenic 30.0%
!! ATGTCCTCTTCTATTTTTCA+TGG - chr4_4:36948617-36948636 Msa0679330:intron 30.0%
! TATGTTTTCCTTCACAAAGA+AGG - chr4_4:36948802-36948821 Msa0679330:intron 30.0%
GAATGATGAGACAAAAAACT+CGG + chr4_4:36948906-36948925 None:intergenic 30.0%
CAAGTTCAATACATTGAAGA+TGG - chr4_4:36949049-36949068 Msa0679330:CDS 30.0%
AATACATTGAAGATGGTGTT+TGG - chr4_4:36949056-36949075 Msa0679330:CDS 30.0%
! TCACTCTTTCTTTTGAACTT+GGG + chr4_4:36949615-36949634 None:intergenic 30.0%
! TTCACTCTTTCTTTTGAACT+TGG + chr4_4:36949616-36949635 None:intergenic 30.0%
ATGTGATTCATTTACATACC+AGG + chr4_4:36949759-36949778 None:intergenic 30.0%
ATTCTAATCAGAAAGCCAAA+CGG + chr4_4:36949797-36949816 None:intergenic 30.0%
CTATTCGACAATACTATATC+TGG + chr4_4:36949844-36949863 None:intergenic 30.0%
TATACATGCAGATTCATACA+AGG - chr4_4:36949982-36950001 Msa0679330:intron 30.0%
TACACTTCATGATGTCAATA+TGG - chr4_4:36950189-36950208 Msa0679330:intron 30.0%
!!! TTTTTTCTGATTCTACTGCT+GGG + chr4_4:36950305-36950324 None:intergenic 30.0%
!!! ATTTTTTCTGATTCTACTGC+TGG + chr4_4:36950306-36950325 None:intergenic 30.0%
!!! CTGTTTTTTAACCATAGTGT+TGG - chr4_4:36950350-36950369 Msa0679330:intron 30.0%
!! TTTTAATTGTACAGACTGAC+TGG - chr4_4:36950654-36950673 Msa0679330:five_prime_UTR 30.0%
TCTTGAACAGAAAAGGTTAA+GGG + chr4_4:36950691-36950710 None:intergenic 30.0%
! TTTTTCCTCTTGAACAGAAA+AGG + chr4_4:36950698-36950717 None:intergenic 30.0%
TTTCTGTTCAAGAGGAAAAA+CGG - chr4_4:36950698-36950717 Msa0679330:five_prime_UTR 30.0%
CTTCTCTTCTCTCATTCATT+CGG - chr4_4:36946476-36946495 Msa0679330:three_prime_UTR 35.0%
CTTCTTGTTCTTGAACTACT+AGG - chr4_4:36946515-36946534 Msa0679330:intron 35.0%
!!! GTTTTGTTTCGATCTGAACA+AGG - chr4_4:36946542-36946561 Msa0679330:intron 35.0%
CTGAACAAGGATAAGATGTT+TGG - chr4_4:36946555-36946574 Msa0679330:intron 35.0%
!! CTCGTACTATCACTTTTCTT+CGG + chr4_4:36946588-36946607 None:intergenic 35.0%
! ACGAGGTACTATGAAATCTT+AGG - chr4_4:36946603-36946622 Msa0679330:three_prime_UTR 35.0%
! TTGGTGATTGATTTAGGGTT+AGG - chr4_4:36946883-36946902 Msa0679330:CDS 35.0%
! TGGTGATTGATTTAGGGTTA+GGG - chr4_4:36946884-36946903 Msa0679330:CDS 35.0%
! ATTGATTTAGGGTTAGGGTT+AGG - chr4_4:36946889-36946908 Msa0679330:CDS 35.0%
ATGATCAAATTACGCCATGA+AGG + chr4_4:36947032-36947051 None:intergenic 35.0%
AAGCGCTTATGTATAAGCTA+TGG - chr4_4:36947130-36947149 Msa0679330:intron 35.0%
AGCGCTTATGTATAAGCTAT+GGG - chr4_4:36947131-36947150 Msa0679330:intron 35.0%
TATTGTTCATAAGCCTGTCA+AGG - chr4_4:36947220-36947239 Msa0679330:intron 35.0%
TTCTCATAAACTACCTTGAC+AGG + chr4_4:36947236-36947255 None:intergenic 35.0%
!! TTCATGGTGTTGTTATTGTG+AGG - chr4_4:36947596-36947615 Msa0679330:intron 35.0%
TCATAAATATCACGCTTCTC+AGG + chr4_4:36947730-36947749 None:intergenic 35.0%
AAGACGAGAAGATGTCAAAT+GGG + chr4_4:36947813-36947832 None:intergenic 35.0%
AAAGACGAGAAGATGTCAAA+TGG + chr4_4:36947814-36947833 None:intergenic 35.0%
ACCGAACCAAATAACACAAT+AGG + chr4_4:36947851-36947870 None:intergenic 35.0%
! GGTCTTAATCGATTTCTGTA+TGG - chr4_4:36947868-36947887 Msa0679330:CDS 35.0%
!!! GGTTTTTGACTTGTATGAAC+AGG - chr4_4:36947906-36947925 Msa0679330:intron 35.0%
!!! TTTTGACTTGTATGAACAGG+TGG - chr4_4:36947909-36947928 Msa0679330:intron 35.0%
AAAGCTTCTTAGATGTTCCA+AGG + chr4_4:36948022-36948041 None:intergenic 35.0%
!!! GTATCGTAAGAGTTTTGGTT+TGG - chr4_4:36948083-36948102 Msa0679330:intron 35.0%
! ATTTTTCATCCTGGAATGTG+AGG - chr4_4:36948185-36948204 Msa0679330:intron 35.0%
! TTGTAAACACATTTGGTGAC+TGG - chr4_4:36948249-36948268 Msa0679330:intron 35.0%
! TGTAAACACATTTGGTGACT+GGG - chr4_4:36948250-36948269 Msa0679330:intron 35.0%
! TTTCTTTTCGTCCCTTCAAA+TGG - chr4_4:36948498-36948517 Msa0679330:intron 35.0%
TTGAAAATCAGATGGGTGTA+AGG + chr4_4:36948584-36948603 None:intergenic 35.0%
!!! TGCACTGATTTTGTTTTGGA+CGG - chr4_4:36948694-36948713 Msa0679330:intron 35.0%
!! TACAGCAAAGGTTCTAAATC+TGG - chr4_4:36949194-36949213 Msa0679330:intron 35.0%
!! ACAGCAAAGGTTCTAAATCT+GGG - chr4_4:36949195-36949214 Msa0679330:intron 35.0%
AAGGTTTCTATTAGACACCT+TGG - chr4_4:36949257-36949276 Msa0679330:CDS 35.0%
CATCCTTGCAATGAATGTAA+GGG - chr4_4:36949305-36949324 Msa0679330:CDS 35.0%
! CACTCTTTCTTTTGAACTTG+GGG + chr4_4:36949614-36949633 None:intergenic 35.0%
! GGTTCGATTTGATAAGAAGT+TGG + chr4_4:36949719-36949738 None:intergenic 35.0%
ACTATAGCAATGTAGCATAG+TGG - chr4_4:36949877-36949896 Msa0679330:intron 35.0%
ATACAAGGCTATTAACGATG+AGG - chr4_4:36949997-36950016 Msa0679330:intron 35.0%
TACAAGGCTATTAACGATGA+GGG - chr4_4:36949998-36950017 Msa0679330:intron 35.0%
TAAAGCTTGCCCTTCATAAA+TGG + chr4_4:36950043-36950062 None:intergenic 35.0%
AATTGTGATGAAGGTTTAGC+TGG + chr4_4:36950136-36950155 None:intergenic 35.0%
ATGTCTGTCAATTGTGATGA+AGG + chr4_4:36950145-36950164 None:intergenic 35.0%
TTCATCACAATTGACAGACA+TGG - chr4_4:36950144-36950163 Msa0679330:intron 35.0%
TCATGATGTCAATATGGAAG+AGG - chr4_4:36950195-36950214 Msa0679330:intron 35.0%
TCAATATGGAAGAGGAGAAT+AGG - chr4_4:36950203-36950222 Msa0679330:intron 35.0%
CATTGAACCAAACACTACAT+AGG + chr4_4:36950382-36950401 None:intergenic 35.0%
! CTATGTAGTGTTTGGTTCAA+TGG - chr4_4:36950380-36950399 Msa0679330:CDS 35.0%
AAGTATTTCGGATGTCAGAA+TGG + chr4_4:36950462-36950481 None:intergenic 35.0%
TCATCATAAAGTAGCACACT+GGG + chr4_4:36950547-36950566 None:intergenic 35.0%
ATCATCATAAAGTAGCACAC+TGG + chr4_4:36950548-36950567 None:intergenic 35.0%
CTCTTGAACAGAAAAGGTTA+AGG + chr4_4:36950692-36950711 None:intergenic 35.0%
! CTTAACCTTTTCTGTTCAAG+AGG - chr4_4:36950690-36950709 Msa0679330:five_prime_UTR 35.0%
TTCTGTTCAAGAGGAAAAAC+GGG - chr4_4:36950699-36950718 Msa0679330:five_prime_UTR 35.0%
TGGGATTGTGTTTGAGCTTT+GGG + chr4_4:36946388-36946407 None:intergenic 40.0%
TAAGAGTGAGATGGAATGGA+TGG + chr4_4:36946412-36946431 None:intergenic 40.0%
!! GCGAAGTTTGTTATAGTGGT+TGG + chr4_4:36946441-36946460 None:intergenic 40.0%
!! ACACGCGAAGTTTGTTATAG+TGG + chr4_4:36946445-36946464 None:intergenic 40.0%
CGAAGAAAAGTGATAGTACG+AGG - chr4_4:36946586-36946605 Msa0679330:intron 40.0%
AAGAATCATCCTGATAAGGG+TGG - chr4_4:36946684-36946703 Msa0679330:three_prime_UTR 40.0%
GCGTAATTTGATCATGTAGC+CGG - chr4_4:36947037-36947056 Msa0679330:CDS 40.0%
TTATCCCATTCCCACTAAGT+CGG + chr4_4:36947064-36947083 None:intergenic 40.0%
TTATAGACATAGGGCTCGTT+TGG + chr4_4:36947364-36947383 None:intergenic 40.0%
AGACTGTTTCGGAGAACTTA+AGG + chr4_4:36947514-36947533 None:intergenic 40.0%
AAGCACTTGTGAGACTGTTT+CGG + chr4_4:36947525-36947544 None:intergenic 40.0%
ATATGGTGAGGATGCTCTTA+AGG - chr4_4:36947753-36947772 Msa0679330:CDS 40.0%
GATGCTCTTAAGGAAGGAAT+GGG - chr4_4:36947763-36947782 Msa0679330:CDS 40.0%
AGAAGATGTCAAATGGGTCA+TGG + chr4_4:36947807-36947826 None:intergenic 40.0%
GACATCTTCTCGTCTTTCTT+TGG - chr4_4:36947817-36947836 Msa0679330:CDS 40.0%
! TCTTTGGAGGATCTTTACCT+TGG - chr4_4:36948002-36948021 Msa0679330:intron 40.0%
!! GGTAGGTATCGTAAGAGTTT+TGG - chr4_4:36948078-36948097 Msa0679330:intron 40.0%
! TTTTGTTGAGCCGAAACTGT+AGG - chr4_4:36948282-36948301 Msa0679330:intron 40.0%
TTTGTTGAGCCGAAACTGTA+GGG - chr4_4:36948283-36948302 Msa0679330:intron 40.0%
CTCTTTACCCAACAACAAGA+AGG - chr4_4:36948390-36948409 Msa0679330:intron 40.0%
!! GTTCTGACCTTCTTGTTGTT+GGG + chr4_4:36948400-36948419 None:intergenic 40.0%
! AGTTCTGACCTTCTTGTTGT+TGG + chr4_4:36948401-36948420 None:intergenic 40.0%
ACAAGAAGGTCAGAACTTGA+AGG - chr4_4:36948404-36948423 Msa0679330:intron 40.0%
GCAAAACCCTTTGGTGAAAA+GGG + chr4_4:36948440-36948459 None:intergenic 40.0%
AGCAAAACCCTTTGGTGAAA+AGG + chr4_4:36948441-36948460 None:intergenic 40.0%
! TTCACCAAAGGGTTTTGCTT+TGG - chr4_4:36948442-36948461 Msa0679330:intron 40.0%
GATACCAAAGCAAAACCCTT+TGG + chr4_4:36948449-36948468 None:intergenic 40.0%
TTCGTCCCTTCAAATGGAAT+TGG - chr4_4:36948504-36948523 Msa0679330:intron 40.0%
TCGTCCCTTCAAATGGAATT+GGG - chr4_4:36948505-36948524 Msa0679330:intron 40.0%
TTTGGAAAGATCTGTCACTG+TGG + chr4_4:36948542-36948561 None:intergenic 40.0%
!!! GCACTGATTTTGTTTTGGAC+GGG - chr4_4:36948695-36948714 Msa0679330:intron 40.0%
!!! CACTGATTTTGTTTTGGACG+GGG - chr4_4:36948696-36948715 Msa0679330:intron 40.0%
ACAGAGATCCTTCTTTGTGA+AGG + chr4_4:36948813-36948832 None:intergenic 40.0%
AGATTTGTGTAATTGGGCAG+CGG - chr4_4:36948848-36948867 Msa0679330:intron 40.0%
ATCTGTGGATTCCATCTCTA+CGG + chr4_4:36948882-36948901 None:intergenic 40.0%
!! CAGCAAAGGTTCTAAATCTG+GGG - chr4_4:36949196-36949215 Msa0679330:intron 40.0%
TTGACAACCAGCACATTTCA+TGG + chr4_4:36949225-36949244 None:intergenic 40.0%
!! ATGAAATGTGCTGGTTGTCA+AGG - chr4_4:36949224-36949243 Msa0679330:intron 40.0%
TTGTCAAGGAACTGGTATGA+AGG - chr4_4:36949238-36949257 Msa0679330:intron 40.0%
! GCATTTGCTGGATCATTGAT+GGG + chr4_4:36949283-36949302 None:intergenic 40.0%
! TGCATTTGCTGGATCATTGA+TGG + chr4_4:36949284-36949303 None:intergenic 40.0%
GCATCCTTGCAATGAATGTA+AGG - chr4_4:36949304-36949323 Msa0679330:CDS 40.0%
GTACCCTTACATTCATTGCA+AGG + chr4_4:36949311-36949330 None:intergenic 40.0%
! TGCAATGAATGTAAGGGTAC+CGG - chr4_4:36949311-36949330 Msa0679330:CDS 40.0%
CTTGAAGTTCATGTCGAGAA+AGG - chr4_4:36949404-36949423 Msa0679330:intron 40.0%
AGTCAGAAGATTACATTCCC+TGG - chr4_4:36949437-36949456 Msa0679330:intron 40.0%
CTCTCAACATACTGCTTCAT+CGG + chr4_4:36949468-36949487 None:intergenic 40.0%
ACAGTGTGTGCTCTACAAAA+AGG + chr4_4:36949641-36949660 None:intergenic 40.0%
CCAAGTGAGTAAGCACAAAT+TGG + chr4_4:36949689-36949708 None:intergenic 40.0%
CCAATTTGTGCTTACTCACT+TGG - chr4_4:36949686-36949705 Msa0679330:intron 40.0%
TTTGTGCTTACTCACTTGGA+TGG - chr4_4:36949690-36949709 Msa0679330:intron 40.0%
CTTCTTATCAAATCGAACCC+CGG - chr4_4:36949720-36949739 Msa0679330:intron 40.0%
TAATCAGAAAGCCAAACGGA+GGG + chr4_4:36949793-36949812 None:intergenic 40.0%
ATGTGTTGTCAGTTGAGCAT+TGG + chr4_4:36949927-36949946 None:intergenic 40.0%
ATAAATGGCCTCTGGTACAT+CGG + chr4_4:36950028-36950047 None:intergenic 40.0%
TACCAGAGGCCATTTATGAA+GGG - chr4_4:36950031-36950050 Msa0679330:intron 40.0%
GCTTTACATTCACTTCACTG+TGG - chr4_4:36950057-36950076 Msa0679330:intron 40.0%
! TGATCAAGACTTAGAGTGTC+GGG + chr4_4:36950088-36950107 None:intergenic 40.0%
CTGATCAAGACTTAGAGTGT+CGG + chr4_4:36950089-36950108 None:intergenic 40.0%
CTAAGTCTTGATCAGGTGAA+AGG - chr4_4:36950094-36950113 Msa0679330:intron 40.0%
ATATGGAAGAGGAGAATAGG+AGG - chr4_4:36950206-36950225 Msa0679330:intron 40.0%
ATAGGTTCTGTCCAACACTA+TGG + chr4_4:36950364-36950383 None:intergenic 40.0%
GACAGAACCTATGTAGTGTT+TGG - chr4_4:36950372-36950391 Msa0679330:CDS 40.0%
GTCTAGAAGAGTGGGAAGTA+CGG + chr4_4:36946350-36946369 None:intergenic 45.0%
GAAGAAGGGTCTAGAAGAGT+GGG + chr4_4:36946358-36946377 None:intergenic 45.0%
CTGGGATTGTGTTTGAGCTT+TGG + chr4_4:36946389-36946408 None:intergenic 45.0%
TATCCCATTCCCACTAAGTC+GGG + chr4_4:36947063-36947082 None:intergenic 45.0%
GTATAAGCTATGGGTCCGTT+TGG - chr4_4:36947140-36947159 Msa0679330:intron 45.0%
AGAGCTAGCACAAGCTTATG+AGG - chr4_4:36947693-36947712 Msa0679330:CDS 45.0%
GGATGCTCTTAAGGAAGGAA+TGG - chr4_4:36947762-36947781 Msa0679330:CDS 45.0%
! TGACTTGTATGAACAGGTGG+TGG - chr4_4:36947912-36947931 Msa0679330:intron 45.0%
AGATGTGGTTCATCCTCTCA+AGG - chr4_4:36947977-36947996 Msa0679330:intron 45.0%
TCATCCTCTCAAGGTCTCTT+TGG - chr4_4:36947986-36948005 Msa0679330:intron 45.0%
TCTTGTGCTCAAAGTGCAGT+GGG - chr4_4:36948057-36948076 Msa0679330:intron 45.0%
CAATCACTGCCCTACAGTTT+CGG + chr4_4:36948295-36948314 None:intergenic 45.0%
GGCAGTGATTGAAATGAGAG+AGG - chr4_4:36948304-36948323 Msa0679330:intron 45.0%
AAATGAGAGAGGCCTTGAGT+GGG - chr4_4:36948315-36948334 Msa0679330:intron 45.0%
GAATTGGGTATTCCCACTCA+AGG + chr4_4:36948330-36948349 None:intergenic 45.0%
ATTCCATCTCTACGGTTGTC+TGG + chr4_4:36948874-36948893 None:intergenic 45.0%
GACAAAAAACTCGGCATCTG+TGG + chr4_4:36948897-36948916 None:intergenic 45.0%
ATATCCCCAGTAACTGTGTC+TGG + chr4_4:36949564-36949583 None:intergenic 45.0%
! TCTCTTACTGAGGCTTTGTG+TGG - chr4_4:36949660-36949679 Msa0679330:intron 45.0%
CTAATCAGAAAGCCAAACGG+AGG + chr4_4:36949794-36949813 None:intergenic 45.0%
GTACCAGAGGCCATTTATGA+AGG - chr4_4:36950030-36950049 Msa0679330:intron 45.0%
!! GATCAAGACTTAGAGTGTCG+GGG + chr4_4:36950087-36950106 None:intergenic 45.0%
CGACACTCTAAGTCTTGATC+AGG - chr4_4:36950087-36950106 Msa0679330:intron 45.0%
TGATCAGGTGAAAGGTTTGC+AGG - chr4_4:36950102-36950121 Msa0679330:intron 45.0%
! ACAACAGGAAGCATATGACG+AGG - chr4_4:36950240-36950259 Msa0679330:intron 45.0%
AGGATGATGATATGCCTGGT+GGG - chr4_4:36950260-36950279 Msa0679330:intron 45.0%
GGAAGAAGGGTCTAGAAGAG+TGG + chr4_4:36946359-36946378 None:intergenic 50.0%
TCTTCTAGACCCTTCTTCCG+TGG - chr4_4:36946360-36946379 Msa0679330:three_prime_UTR 50.0%
CTTCTAGACCCTTCTTCCGT+GGG - chr4_4:36946361-36946380 Msa0679330:three_prime_UTR 50.0%
!! TGTTTGAGCTTTGGGTCCCA+CGG + chr4_4:36946380-36946399 None:intergenic 50.0%
GTGAGATGGAATGGATGGCT+GGG + chr4_4:36946407-36946426 None:intergenic 50.0%
AGTGAGATGGAATGGATGGC+TGG + chr4_4:36946408-36946427 None:intergenic 50.0%
GACATAGGGCTCGTTTGGAT+TGG + chr4_4:36947359-36947378 None:intergenic 50.0%
GGTGAGGATGCTCTTAAGGA+AGG - chr4_4:36947757-36947776 Msa0679330:CDS 50.0%
GCTCTTAAGGAAGGAATGGG+TGG - chr4_4:36947766-36947785 Msa0679330:CDS 50.0%
CTTAAGGAAGGAATGGGTGG+TGG - chr4_4:36947769-36947788 Msa0679330:CDS 50.0%
! CTTGTATGAACAGGTGGTGG+TGG - chr4_4:36947915-36947934 Msa0679330:intron 50.0%
TCCTCTCAAGGTCTCTTTGG+AGG - chr4_4:36947989-36948008 Msa0679330:intron 50.0%
TCCTCCAAAGAGACCTTGAG+AGG + chr4_4:36947993-36948012 None:intergenic 50.0%
GTCTTGTGCTCAAAGTGCAG+TGG - chr4_4:36948056-36948075 Msa0679330:intron 50.0%
GTGAGAATGCCTCACATTCC+AGG + chr4_4:36948197-36948216 None:intergenic 50.0%
GAAATGAGAGAGGCCTTGAG+TGG - chr4_4:36948314-36948333 Msa0679330:intron 50.0%
! CTTTGGTGAAAAGGGTGTGG+CGG + chr4_4:36948432-36948451 None:intergenic 50.0%
! GCCACACCCTTTTCACCAAA+GGG - chr4_4:36948431-36948450 Msa0679330:intron 50.0%
ACCCTTTGGTGAAAAGGGTG+TGG + chr4_4:36948435-36948454 None:intergenic 50.0%
!! TGTGCTGGTTGTCAAGGAAC+TGG - chr4_4:36949230-36949249 Msa0679330:intron 50.0%
TGCAAGGATGCTGCATTTGC+TGG + chr4_4:36949295-36949314 None:intergenic 50.0%
TTGCAGCCAGACACAGTTAC+TGG - chr4_4:36949555-36949574 Msa0679330:intron 50.0%
TGCAGCCAGACACAGTTACT+GGG - chr4_4:36949556-36949575 Msa0679330:intron 50.0%
GCACACACTGTCTCTTACTG+AGG - chr4_4:36949650-36949669 Msa0679330:intron 50.0%
ACCAGGCTTGACAACTTCTC+CGG + chr4_4:36949742-36949761 None:intergenic 50.0%
CTAGCATGTTACCCTCCGTT+TGG - chr4_4:36949779-36949798 Msa0679330:intron 50.0%
TGCCCTTCATAAATGGCCTC+TGG + chr4_4:36950036-36950055 None:intergenic 50.0%
GAGGAAACAGCAGCAACAAC+AGG - chr4_4:36950225-36950244 Msa0679330:intron 50.0%
GACGAGGATGATGATATGCC+TGG - chr4_4:36950256-36950275 Msa0679330:intron 50.0%
GAGGATGATGATATGCCTGG+TGG - chr4_4:36950259-36950278 Msa0679330:intron 50.0%
GGATGATGATATGCCTGGTG+GGG - chr4_4:36950261-36950280 Msa0679330:intron 50.0%
CTTTGGGTCCCACGGAAGAA+GGG + chr4_4:36946372-36946391 None:intergenic 55.0%
TCGGGATCACCACCCTTATC+AGG + chr4_4:36946696-36946715 None:intergenic 55.0%
TAAGGGTGGTGATCCCGAGA+AGG - chr4_4:36946698-36946717 Msa0679330:three_prime_UTR 55.0%
! AAGGAAGGAATGGGTGGTGG+AGG - chr4_4:36947772-36947791 Msa0679330:CDS 55.0%
!! GTATGAACAGGTGGTGGTGG+TGG - chr4_4:36947918-36947937 Msa0679330:intron 55.0%
GCAGAGACGTGGAGAAGATG+TGG - chr4_4:36947962-36947981 Msa0679330:intron 55.0%
GTGCTCAAAGTGCAGTGGGT+AGG - chr4_4:36948061-36948080 Msa0679330:intron 55.0%
! CGCCACACCCTTTTCACCAA+AGG - chr4_4:36948430-36948449 Msa0679330:intron 55.0%
GGGGCTTCCATGAAATGTGC+TGG - chr4_4:36949215-36949234 Msa0679330:intron 55.0%
! GCTGGATCATTGATGGGCCA+AGG + chr4_4:36949277-36949296 None:intergenic 55.0%
TCCACAGTGCAAGGGAGAGA+AGG - chr4_4:36949361-36949380 Msa0679330:CDS 55.0%
ACCTTCTCTCCCTTGCACTG+TGG + chr4_4:36949365-36949384 None:intergenic 55.0%
!! CTGCTTCATCGGCTTCACCA+GGG + chr4_4:36949457-36949476 None:intergenic 55.0%
!! ACTGCTTCATCGGCTTCACC+AGG + chr4_4:36949458-36949477 None:intergenic 55.0%
GCAGCCAGACACAGTTACTG+GGG - chr4_4:36949557-36949576 Msa0679330:intron 55.0%
CAGGCTTGACAACTTCTCCG+GGG + chr4_4:36949740-36949759 None:intergenic 55.0%
CCAGGCTTGACAACTTCTCC+GGG + chr4_4:36949741-36949760 None:intergenic 55.0%
AGGGAATGCCGATGTACCAG+AGG - chr4_4:36950017-36950036 Msa0679330:intron 55.0%
CATGGAGATCGATGAGTGCG+AGG - chr4_4:36950162-36950181 Msa0679330:intron 55.0%
! GCTTTGGGTCCCACGGAAGA+AGG + chr4_4:36946373-36946392 None:intergenic 60.0%
ATGTAGCCGGCCCGACTTAG+TGG - chr4_4:36947050-36947069 Msa0679330:CDS 60.0%
TGTAGCCGGCCCGACTTAGT+GGG - chr4_4:36947051-36947070 Msa0679330:CDS 60.0%
CCATTCCCACTAAGTCGGGC+CGG + chr4_4:36947059-36947078 None:intergenic 60.0%
CGGCCCGACTTAGTGGGAAT+GGG - chr4_4:36947057-36947076 Msa0679330:CDS 60.0%
!! GAAGGAATGGGTGGTGGAGG+CGG - chr4_4:36947775-36947794 Msa0679330:CDS 60.0%
!! TGAACAGGTGGTGGTGGTGG+TGG - chr4_4:36947921-36947940 Msa0679330:intron 60.0%
GTGGTGGTAGTAGCCGAGGA+CGG - chr4_4:36947937-36947956 Msa0679330:intron 60.0%
CGGCCAGACAACCGTAGAGA+TGG - chr4_4:36948868-36948887 Msa0679330:intron 60.0%
AGACCGCTGTCCACAGTGCA+AGG - chr4_4:36949352-36949371 Msa0679330:CDS 60.0%
GACCGCTGTCCACAGTGCAA+GGG - chr4_4:36949353-36949372 Msa0679330:CDS 60.0%
CTCCCTTGCACTGTGGACAG+CGG + chr4_4:36949358-36949377 None:intergenic 60.0%
CCCGGAGAAGTTGTCAAGCC+TGG - chr4_4:36949738-36949757 Msa0679330:intron 60.0%
ATATGCCTGGTGGGGCACAG+AGG - chr4_4:36950269-36950288 Msa0679330:intron 60.0%
TATGCCTGGTGGGGCACAGA+GGG - chr4_4:36950270-36950289 Msa0679330:intron 60.0%
CCGGCCCGACTTAGTGGGAA+TGG - chr4_4:36947056-36947075 Msa0679330:CDS 65.0%
!! GGTGGTGGTGGTAGTAGCCG+AGG - chr4_4:36947933-36947952 Msa0679330:intron 65.0%
GTGGTAGTAGCCGAGGACGG+AGG - chr4_4:36947940-36947959 Msa0679330:intron 65.0%
CACGTCTCTGCCTCCGTCCT+CGG + chr4_4:36947953-36947972 None:intergenic 65.0%
TGTACCCTCTGTGCCCCACC+AGG + chr4_4:36950277-36950296 None:intergenic 65.0%
!! GGAATGGGTGGTGGAGGCGG+TGG - chr4_4:36947778-36947797 Msa0679330:CDS 70.0%
!! ATGGGTGGTGGAGGCGGTGG+TGG - chr4_4:36947781-36947800 Msa0679330:CDS 70.0%
CGAGGACGGAGGCAGAGACG+TGG - chr4_4:36947951-36947970 Msa0679330:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 36946337 36950752 36946337 ID=Msa0679330;Name=Msa0679330
chr4_4 mRNA 36946337 36950752 36946337 ID=Msa0679330-mRNA-1;Parent=Msa0679330;Name=Msa0679330-mRNA-1;_AED=0.04;_eAED=0.04;_QI=233|1|1|1|0.83|0.71|7|349|415
chr4_4 exon 36946337 36946505 36946337 ID=Msa0679330-mRNA-1:exon:8736;Parent=Msa0679330-mRNA-1
chr4_4 exon 36946597 36947096 36946597 ID=Msa0679330-mRNA-1:exon:8735;Parent=Msa0679330-mRNA-1
chr4_4 exon 36947330 36947528 36947330 ID=Msa0679330-mRNA-1:exon:8734;Parent=Msa0679330-mRNA-1
chr4_4 exon 36947614 36947890 36947614 ID=Msa0679330-mRNA-1:exon:8733;Parent=Msa0679330-mRNA-1
chr4_4 exon 36949011 36949161 36949011 ID=Msa0679330-mRNA-1:exon:8732;Parent=Msa0679330-mRNA-1
chr4_4 exon 36949251 36949401 36949251 ID=Msa0679330-mRNA-1:exon:8731;Parent=Msa0679330-mRNA-1
chr4_4 exon 36950370 36950752 36950370 ID=Msa0679330-mRNA-1:exon:8730;Parent=Msa0679330-mRNA-1
chr4_4 five_prime_UTR 36950520 36950752 36950520 ID=Msa0679330-mRNA-1:five_prime_utr;Parent=Msa0679330-mRNA-1
chr4_4 CDS 36950370 36950519 36950370 ID=Msa0679330-mRNA-1:cds;Parent=Msa0679330-mRNA-1
chr4_4 CDS 36949251 36949401 36949251 ID=Msa0679330-mRNA-1:cds;Parent=Msa0679330-mRNA-1
chr4_4 CDS 36949011 36949161 36949011 ID=Msa0679330-mRNA-1:cds;Parent=Msa0679330-mRNA-1
chr4_4 CDS 36947614 36947890 36947614 ID=Msa0679330-mRNA-1:cds;Parent=Msa0679330-mRNA-1
chr4_4 CDS 36947330 36947528 36947330 ID=Msa0679330-mRNA-1:cds;Parent=Msa0679330-mRNA-1
chr4_4 CDS 36946777 36947096 36946777 ID=Msa0679330-mRNA-1:cds;Parent=Msa0679330-mRNA-1
chr4_4 three_prime_UTR 36946597 36946776 36946597 ID=Msa0679330-mRNA-1:three_prime_utr;Parent=Msa0679330-mRNA-1
chr4_4 three_prime_UTR 36946337 36946505 36946337 ID=Msa0679330-mRNA-1:three_prime_utr;Parent=Msa0679330-mRNA-1
Gene Sequence

>Msa0679330

ATGTTTGGAAGAGCACCGAAGAAAAGTGATAGTACGAGGTACTATGAAATCTTAGGTGTATCTAAAACTGCTTCTCAAGATGATTTGAAGAAAGCTTATAAAAAAGCTGCTATTAAGAATCATCCTGATAAGGGTGGTGATCCCGAGAAGTTCAAAGAGCTAGCACAAGCTTATGAGGTTTTGAGTGATCCTGAGAAGCGTGATATTTATGATACATATGGTGAGGATGCTCTTAAGGAAGGAATGGGTGGTGGAGGCGGTGGTGGCCATGACCCATTTGACATCTTCTCGTCTTTCTTTGGTGGTGGTGGTGGTGGTAGTAGCCGAGGACGGAGGCAGAGACGTGGAGAAGATGTGGTTCATCCTCTCAAGGTCTCTTTGGAGGATCTTTACCTTGGAACATCTAAGAAGCTTTCTCTCTCGAGAAATGTCTTGTGCTCAAAGTGCAGTGGCAAAGGTTCTAAATCTGGGGCTTCCATGAAATGTGCTGGTTGTCAAGGAACTGGTATGAAGGTTTCTATTAGACACCTTGGCCCATCAATGATCCAGCAAATGCAGCATCCTTGCAATGAATGTAAGGGTACCGGTGAAACTATCAATGATAAAGACCGCTGTCCACAGTGCAAGGGAGAGAAGGTTGTACAAGAGAAGAAAGTACTTGAAGTTCATGTCGAGAAAGGAATGCAGAACAGTCAGAAGATTACATTCCCTGGTGAAGCCGATGAAGCACCAGACACAGTTACTGGGGATATTGTATTTGTGCTTCAGCAGAAAGAACACCCCAAGTTCAAAAGAAAGAGTGAAGACCTTTTTGTAGAGCACACACTGTCTCTTACTGAGGCTTTGTGTGGTTTCCAATTTGTGCTTACTCACTTGGATGGTCGCCAACTTCTTATCAAATCGAACCCCGGAGAAGTTGTCAAGCCTGATTCATACAAGGCTATTAACGATGAGGGAATGCCGATGTACCAGAGGCCATTTATGAAGGGCAAGCTTTACATTCACTTCACTGTGGAATTCCCCGACACTCTAAGTCTTGATCAGGTGAAAGGTTTGCAGGCTGTTCTTCCAGCTAAACCTTCATCACAATTGACAGACATGGAGATCGATGAGTGCGAGGAAACTACACTTCATGATGTCAATATGGAAGAGGAGAATAGGAGGAAACAGCAGCAACAACAGGAAGCATATGACGAGGATGATGATATGCCTGGTGGGGCACAGAGGGTACAGTGCGCCCAGCAGTAG
Protein sequence

>Msa0679330

MFGRAPKKSDSTRYYEILGVSKTASQDDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKRDIYDTYGEDALKEGMGGGGGGGHDPFDIFSSFFGGGGGGSSRGRRQRRGEDVVHPLKVSLEDLYLGTSKKLSLSRNVLCSKCSGKGSKSGASMKCAGCQGTGMKVSIRHLGPSMIQQMQHPCNECKGTGETINDKDRCPQCKGEKVVQEKKVLEVHVEKGMQNSQKITFPGEADEAPDTVTGDIVFVLQQKEHPKFKRKSEDLFVEHTLSLTEALCGFQFVLTHLDGRQLLIKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHFTVEFPDTLSLDQVKGLQAVLPAKPSSQLTDMEIDECEETTLHDVNMEEENRRKQQQQQEAYDEDDDMPGGAQRVQCAQQ*