Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0683870 | AES88834.2 | 76.768 | 99 | 13 | 3 | 1 | 98 | 1 | 90 | 2.07e-30 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0683870 | G7JTR5 | 76.768 | 99 | 13 | 3 | 1 | 98 | 1 | 90 | 9.89e-31 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0603000 | Msa0683870 | 0.902094 | 1.377551e-78 | -8.615850e-47 |
| Msa0603020 | Msa0683870 | 0.889168 | 3.084072e-73 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0683870 | MtrunA17_Chr4g0031021 | 76.768 | 99 | 13 | 3 | 1 | 98 | 1 | 90 | 1.90e-34 | 114 |
| Msa0683870 | MtrunA17_Chr4g0031011 | 51.546 | 97 | 40 | 2 | 1 | 94 | 1 | 93 | 2.95e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGGTCTGGGTGAGAAGTT+TGG | 0.374289 | 4_4:+46968841 | Msa0683870:CDS |
| AAGGCAATGTCATGTGAGTA+TGG | 0.402722 | 4_4:-46968780 | None:intergenic |
| CAACACTAAGGTCATGGCTT+TGG | 0.441416 | 4_4:-46968677 | None:intergenic |
| ATGATGTAAACGACGTTGCT+AGG | 0.472531 | 4_4:+46968923 | Msa0683870:CDS |
| CACTACGGTGCTTTCTGAAG+AGG | 0.485776 | 4_4:+46968726 | Msa0683870:CDS |
| TCCACCGGTAGACGCATGCT+AGG | 0.507807 | 4_4:+46968802 | Msa0683870:CDS |
| GAACCCTAGCATGCGTCTAC+CGG | 0.513042 | 4_4:-46968806 | None:intergenic |
| CATTCAATCATTGTTATCAT+CGG | 0.520776 | 4_4:-46968953 | None:intergenic |
| TCTACAGTTGCATCAAACTT+CGG | 0.536605 | 4_4:-46968865 | None:intergenic |
| TGGGTAGGAGAGTAAGTGGT+GGG | 0.540063 | 4_4:-46968760 | None:intergenic |
| TGATGTAAACGACGTTGCTA+GGG | 0.561947 | 4_4:+46968924 | Msa0683870:CDS |
| CACATGACATTGCCTTCCAC+CGG | 0.580768 | 4_4:+46968787 | Msa0683870:CDS |
| CCCTAGCATGCGTCTACCGG+TGG | 0.591972 | 4_4:-46968803 | None:intergenic |
| GAGGAGCAACACTAAGGTCA+TGG | 0.596052 | 4_4:-46968683 | None:intergenic |
| TAGGAGGAGGAGCAACACTA+AGG | 0.598370 | 4_4:-46968689 | None:intergenic |
| CCACCGGTAGACGCATGCTA+GGG | 0.606573 | 4_4:+46968803 | Msa0683870:CDS |
| TAGTGAAAAGAGACAAAACT+AGG | 0.608337 | 4_4:-46968708 | None:intergenic |
| AGTATGGGTAGGAGAGTAAG+TGG | 0.611157 | 4_4:-46968764 | None:intergenic |
| AGCATGCGTCTACCGGTGGA+AGG | 0.612142 | 4_4:-46968799 | None:intergenic |
| AAAGAGACAAAACTAGGAGG+AGG | 0.615522 | 4_4:-46968702 | None:intergenic |
| AATGTCATGTGAGTATGGGT+AGG | 0.634885 | 4_4:-46968775 | None:intergenic |
| ATGGGTAGGAGAGTAAGTGG+TGG | 0.647747 | 4_4:-46968761 | None:intergenic |
| AGGCAATGTCATGTGAGTAT+GGG | 0.653460 | 4_4:-46968779 | None:intergenic |
| GATGCAACTGTAGAAAAGCA+TGG | 0.685732 | 4_4:+46968874 | Msa0683870:CDS |
| TGAAAAGAGACAAAACTAGG+AGG | 0.694756 | 4_4:-46968705 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAGTGAAAAGAGACAAAACT+AGG | - | chr4_4:46968711-46968730 | None:intergenic | 30.0% | |
| !! | AGTTTTGTCTCTTTTCACTA+CGG | + | chr4_4:46968711-46968730 | Msa0683870:CDS | 30.0% |
| TGAAAAGAGACAAAACTAGG+AGG | - | chr4_4:46968708-46968727 | None:intergenic | 35.0% | |
| TCTACAGTTGCATCAAACTT+CGG | - | chr4_4:46968868-46968887 | None:intergenic | 35.0% | |
| AAAGAGACAAAACTAGGAGG+AGG | - | chr4_4:46968705-46968724 | None:intergenic | 40.0% | |
| AATGTCATGTGAGTATGGGT+AGG | - | chr4_4:46968778-46968797 | None:intergenic | 40.0% | |
| AGGCAATGTCATGTGAGTAT+GGG | - | chr4_4:46968782-46968801 | None:intergenic | 40.0% | |
| AAGGCAATGTCATGTGAGTA+TGG | - | chr4_4:46968783-46968802 | None:intergenic | 40.0% | |
| GATGCAACTGTAGAAAAGCA+TGG | + | chr4_4:46968874-46968893 | Msa0683870:CDS | 40.0% | |
| ATGATGTAAACGACGTTGCT+AGG | + | chr4_4:46968923-46968942 | Msa0683870:CDS | 40.0% | |
| TGATGTAAACGACGTTGCTA+GGG | + | chr4_4:46968924-46968943 | Msa0683870:CDS | 40.0% | |
| CAACACTAAGGTCATGGCTT+TGG | - | chr4_4:46968680-46968699 | None:intergenic | 45.0% | |
| AGTATGGGTAGGAGAGTAAG+TGG | - | chr4_4:46968767-46968786 | None:intergenic | 45.0% | |
| GAGGAGCAACACTAAGGTCA+TGG | - | chr4_4:46968686-46968705 | None:intergenic | 50.0% | |
| TAGGAGGAGGAGCAACACTA+AGG | - | chr4_4:46968692-46968711 | None:intergenic | 50.0% | |
| ! | CACTACGGTGCTTTCTGAAG+AGG | + | chr4_4:46968726-46968745 | Msa0683870:CDS | 50.0% |
| TGGGTAGGAGAGTAAGTGGT+GGG | - | chr4_4:46968763-46968782 | None:intergenic | 50.0% | |
| ATGGGTAGGAGAGTAAGTGG+TGG | - | chr4_4:46968764-46968783 | None:intergenic | 50.0% | |
| CACATGACATTGCCTTCCAC+CGG | + | chr4_4:46968787-46968806 | Msa0683870:CDS | 50.0% | |
| !!! | AGGGTTCGCCAGCTTTTTCA+AGG | + | chr4_4:46968822-46968841 | Msa0683870:CDS | 50.0% |
| !!! | GGGTTCGCCAGCTTTTTCAA+GGG | + | chr4_4:46968823-46968842 | Msa0683870:CDS | 50.0% |
| !!! | GCCAGCTTTTTCAAGGGTCT+GGG | + | chr4_4:46968829-46968848 | Msa0683870:CDS | 50.0% |
| ACCCAGACCCTTGAAAAAGC+TGG | - | chr4_4:46968833-46968852 | None:intergenic | 50.0% | |
| AAGGGTCTGGGTGAGAAGTT+TGG | + | chr4_4:46968841-46968860 | Msa0683870:CDS | 50.0% | |
| GAACCCTAGCATGCGTCTAC+CGG | - | chr4_4:46968809-46968828 | None:intergenic | 55.0% | |
| !!! | CGCCAGCTTTTTCAAGGGTC+TGG | + | chr4_4:46968828-46968847 | Msa0683870:CDS | 55.0% |
| AGCATGCGTCTACCGGTGGA+AGG | - | chr4_4:46968802-46968821 | None:intergenic | 60.0% | |
| TCCACCGGTAGACGCATGCT+AGG | + | chr4_4:46968802-46968821 | Msa0683870:CDS | 60.0% | |
| CCACCGGTAGACGCATGCTA+GGG | + | chr4_4:46968803-46968822 | Msa0683870:CDS | 60.0% | |
| CCCTAGCATGCGTCTACCGG+TGG | - | chr4_4:46968806-46968825 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 46968673 | 46968972 | 46968673 | ID=Msa0683870;Name=Msa0683870 |
| chr4_4 | mRNA | 46968673 | 46968972 | 46968673 | ID=Msa0683870-mRNA-1;Parent=Msa0683870;Name=Msa0683870-mRNA-1;_AED=0.39;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|99 |
| chr4_4 | exon | 46968673 | 46968972 | 46968673 | ID=Msa0683870-mRNA-1:exon:11555;Parent=Msa0683870-mRNA-1 |
| chr4_4 | CDS | 46968673 | 46968972 | 46968673 | ID=Msa0683870-mRNA-1:cds;Parent=Msa0683870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
