AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0683940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683940 MtrunA17_Chr4g0031091 93.750 112 7 0 1 112 1 112 4.65e-77 222
Msa0683940 MtrunA17_Chr3g0121631 46.000 100 53 1 12 111 15 113 6.54e-27 97.1
Msa0683940 MtrunA17_Chr7g0219021 41.935 93 54 0 17 109 24 116 8.88e-24 91.7
Msa0683940 MtrunA17_Chr4g0061451 43.333 90 51 0 22 111 31 120 1.09e-23 91.7
Msa0683940 MtrunA17_Chr3g0086861 41.237 97 54 1 18 111 26 122 5.89e-21 86.3
Msa0683940 MtrunA17_Chr7g0266751 36.792 106 67 0 4 109 3 108 1.98e-20 80.1
Msa0683940 MtrunA17_Chr3g0119311 37.662 77 48 0 33 109 36 112 2.57e-18 74.7
Msa0683940 MtrunA17_Chr1g0198431 46.053 76 41 0 34 109 193 268 7.80e-18 76.3
Msa0683940 MtrunA17_Chr7g0219031 34.940 83 54 0 17 99 24 106 7.06e-17 70.5
Msa0683940 MtrunA17_Chr4g0065741 41.463 82 48 0 31 112 69 150 1.07e-16 71.2
Msa0683940 MtrunA17_Chr4g0035051 39.744 78 47 0 34 111 9 86 1.87e-16 69.3
Msa0683940 MtrunA17_Chr4g0035001 38.961 77 47 0 34 110 7 83 6.59e-16 68.9
Msa0683940 MtrunA17_Chr2g0327921 44.156 77 43 0 33 109 7 83 8.30e-16 67.0
Msa0683940 MtrunA17_Chr4g0035031 40.260 77 46 0 34 110 9 85 3.52e-15 67.8
Msa0683940 MtrunA17_Chr3g0127751 41.772 79 45 1 33 111 26 103 6.97e-15 67.8
Msa0683940 MtrunA17_Chr8g0346011 45.000 80 43 1 33 112 37 115 1.92e-14 67.4
Msa0683940 MtrunA17_Chr6g0481071 35.065 77 50 0 33 109 95 171 1.99e-14 65.9
Msa0683940 MtrunA17_Chr3g0144311 46.479 71 37 1 33 103 29 98 2.59e-14 66.6
Msa0683940 MtrunA17_Chr3g0116631 36.364 77 49 0 34 110 207 283 3.63e-14 66.6
Msa0683940 MtrunA17_Chr2g0278661 38.961 77 47 0 34 110 8 84 3.85e-14 65.9
Msa0683940 MtrunA17_Chr4g0017401 45.070 71 38 1 33 103 16 85 4.32e-14 65.9
Msa0683940 MtrunA17_Chr4g0050781 38.961 77 47 0 34 110 8 84 9.36e-14 64.3
Msa0683940 MtrunA17_Chr7g0263001 40.506 79 47 0 31 109 33 111 1.16e-13 62.8
Msa0683940 MtrunA17_Chr5g0426311 43.662 71 39 1 33 103 16 85 1.34e-13 64.7
Msa0683940 MtrunA17_Chr7g0276531 43.662 71 39 1 33 103 18 87 2.37e-13 63.9
Msa0683940 MtrunA17_Chr1g0201151 39.024 82 49 1 27 108 112 192 2.73e-13 64.7
Msa0683940 MtrunA17_Chr1g0201001 30.000 90 63 0 20 109 16 105 5.36e-13 63.5
Msa0683940 MtrunA17_Chr4g0042161 34.568 81 53 0 30 110 48 128 5.45e-13 61.2
Msa0683940 MtrunA17_Chr4g0058151 30.392 102 71 0 10 111 193 294 6.23e-13 63.2
Msa0683940 MtrunA17_Chr1g0153181 35.802 81 51 1 28 108 115 194 1.54e-12 62.4
Msa0683940 MtrunA17_Chr1g0153181 37.500 80 50 0 32 111 31 110 1.38e-11 59.7
Msa0683940 MtrunA17_Chr4g0044541 37.037 81 50 1 27 107 116 195 3.25e-12 61.6
Msa0683940 MtrunA17_Chr7g0276201 35.802 81 52 0 25 105 96 176 5.05e-12 60.8
Msa0683940 MtrunA17_Chr4g0050441 34.615 78 51 0 35 112 18 95 7.65e-12 60.1
Msa0683940 MtrunA17_Chr1g0181111 36.486 74 47 0 37 110 200 273 1.09e-11 59.7
Msa0683940 MtrunA17_Chr5g0429941 34.615 78 51 0 35 112 18 95 1.16e-11 59.3
Msa0683940 MtrunA17_Chr2g0290191 38.158 76 45 1 34 109 5 78 1.40e-11 59.7
Msa0683940 MtrunA17_Chr5g0447761 38.667 75 43 1 32 103 5 79 1.47e-11 59.7
Msa0683940 MtrunA17_Chr3g0110211 38.462 78 47 1 35 111 114 191 3.20e-11 58.5
Msa0683940 MtrunA17_Chr1g0168601 32.468 77 52 0 34 110 154 230 3.74e-11 57.8
Msa0683940 MtrunA17_Chr6g0465751 31.522 92 63 0 20 111 12 103 5.06e-11 58.2
Msa0683940 MtrunA17_Chr8g0360551 38.462 104 57 3 5 102 39 141 5.45e-11 57.8
Msa0683940 MtrunA17_Chr4g0045221 37.500 80 49 1 31 109 212 291 7.90e-11 57.4
Msa0683940 MtrunA17_Chr3g0101061 35.526 76 47 1 34 109 41 114 8.20e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683940 AT5G59865.1 56.250 96 41 1 15 110 9 103 7.11e-29 101
Msa0683940 AT4G13850.4 50.000 80 40 0 32 111 34 113 9.22e-24 88.6
Msa0683940 AT4G13850.3 50.000 80 40 0 32 111 34 113 1.24e-23 89.0
Msa0683940 AT4G13850.2 50.000 80 40 0 32 111 34 113 1.56e-23 89.0
Msa0683940 AT4G13850.1 50.000 80 40 0 32 111 34 113 1.77e-23 89.0
Msa0683940 AT5G61030.2 40.187 107 62 1 4 110 13 117 4.60e-22 88.2
Msa0683940 AT5G61030.1 40.187 107 62 1 4 110 13 117 4.60e-22 88.2
Msa0683940 AT1G74230.1 43.956 91 50 1 19 109 20 109 6.60e-21 85.1
Msa0683940 AT3G23830.2 43.678 87 48 1 23 109 26 111 8.03e-21 81.3
Msa0683940 AT3G23830.1 43.678 87 48 1 23 109 26 111 8.03e-21 81.3
Msa0683940 AT3G08000.1 37.778 90 56 0 20 109 28 117 4.77e-18 74.3
Msa0683940 AT3G20930.1 46.154 78 42 0 32 109 281 358 1.36e-17 77.0
Msa0683940 AT5G06210.1 42.169 83 48 0 28 110 29 111 3.71e-17 72.4
Msa0683940 AT4G39260.3 41.558 77 45 0 34 110 7 83 8.37e-17 70.1
Msa0683940 AT1G73530.1 42.857 77 44 0 33 109 77 153 9.51e-17 72.0
Msa0683940 AT4G39260.2 41.558 77 45 0 34 110 7 83 1.23e-16 70.5
Msa0683940 AT4G39260.1 41.558 77 45 0 34 110 7 83 3.17e-16 70.5
Msa0683940 AT2G21660.2 40.260 77 46 0 34 110 9 85 6.68e-16 69.3
Msa0683940 AT2G21660.1 40.260 77 46 0 34 110 9 85 9.38e-16 69.3
Msa0683940 AT2G27330.1 41.975 81 46 1 24 104 13 92 1.92e-15 67.0
Msa0683940 AT1G76460.1 47.887 71 36 1 33 103 24 93 1.94e-15 70.1
Msa0683940 AT3G20930.2 43.373 83 42 1 32 109 281 363 2.25e-15 70.9
Msa0683940 AT3G46020.1 44.304 79 44 0 32 110 6 84 4.79e-15 65.9
Msa0683940 AT2G46780.1 46.479 71 37 1 33 103 22 91 5.12e-15 69.3
Msa0683940 AT2G46780.2 46.479 71 37 1 33 103 22 91 5.12e-15 69.3
Msa0683940 AT5G47320.1 40.698 86 51 0 24 109 22 107 1.12e-14 67.4
Msa0683940 AT1G33470.2 46.479 71 37 1 33 103 7 76 1.36e-14 67.4
Msa0683940 AT1G33470.3 46.479 71 37 1 33 103 7 76 1.43e-14 67.4
Msa0683940 AT1G33470.1 46.479 71 37 1 33 103 7 76 1.43e-14 67.4
Msa0683940 AT2G37510.1 39.286 84 48 1 26 106 24 107 1.62e-14 65.5
Msa0683940 AT5G11412.1 40.000 80 47 1 33 112 52 130 2.07e-14 65.9
Msa0683940 AT2G37220.1 35.897 78 50 0 33 110 204 281 2.50e-14 67.4
Msa0683940 AT2G37220.1 38.356 73 45 0 34 106 92 164 4.01e-11 58.5
Msa0683940 AT5G54580.1 34.545 110 62 1 2 111 35 134 3.97e-14 64.7
Msa0683940 AT5G19030.1 37.662 77 48 0 36 112 80 156 7.56e-14 64.3
Msa0683940 AT1G20880.2 46.479 71 37 1 33 103 24 93 8.01e-14 65.9
Msa0683940 AT1G20880.3 46.479 71 37 1 33 103 24 93 8.01e-14 65.9
Msa0683940 AT1G20880.1 46.479 71 37 1 33 103 24 93 8.01e-14 65.9
Msa0683940 AT3G16380.1 41.772 79 45 1 33 111 304 381 1.63e-13 65.5
Msa0683940 AT1G78260.1 43.662 71 39 1 33 103 17 86 2.63e-13 64.3
Msa0683940 AT3G53460.1 36.842 76 48 0 34 109 258 333 2.72e-13 64.7
Msa0683940 AT3G53460.3 36.842 76 48 0 34 109 258 333 2.72e-13 64.7
Msa0683940 AT3G53460.2 36.842 76 48 0 34 109 258 333 2.72e-13 64.7
Msa0683940 AT1G78260.2 42.254 71 40 1 33 103 17 86 2.84e-13 64.3
Msa0683940 AT3G53460.4 36.842 76 48 0 34 109 279 354 2.87e-13 64.7
Msa0683940 AT3G26420.1 39.726 73 44 0 34 106 8 80 3.00e-13 63.9
Msa0683940 AT4G00830.3 41.463 82 47 1 24 105 108 188 3.50e-13 64.7
Msa0683940 AT4G00830.5 41.463 82 47 1 24 105 108 188 3.50e-13 64.7
Msa0683940 AT4G00830.2 41.463 82 47 1 24 105 108 188 3.51e-13 64.7
Msa0683940 AT4G00830.1 41.463 82 47 1 24 105 108 188 3.51e-13 64.7
Msa0683940 AT4G00830.6 41.463 82 47 1 24 105 108 188 3.51e-13 64.7
Msa0683940 AT1G22910.2 41.250 80 46 1 33 112 13 91 4.62e-13 63.2
Msa0683940 AT1G22910.1 41.250 80 46 1 33 112 13 91 5.16e-13 63.2
Msa0683940 AT1G22910.3 41.250 80 46 1 33 112 13 91 5.45e-13 63.9
Msa0683940 AT1G22330.1 38.750 80 48 1 33 112 17 95 6.36e-13 63.5
Msa0683940 AT5G50250.1 28.916 83 59 0 29 111 203 285 1.45e-12 62.4
Msa0683940 AT4G39260.4 40.323 62 37 0 34 95 7 68 1.96e-12 58.9
Msa0683940 AT1G18630.1 34.211 76 50 0 31 106 34 109 2.96e-12 59.7
Msa0683940 AT1G54080.1 36.585 82 52 0 31 112 146 227 3.26e-12 62.0
Msa0683940 AT4G20030.1 30.769 104 69 2 12 112 16 119 3.66e-12 59.3
Msa0683940 AT5G53720.1 40.000 80 47 1 33 112 9 87 3.67e-12 58.2
Msa0683940 AT3G14100.1 34.177 79 52 0 34 112 145 223 9.50e-12 60.5
Msa0683940 AT1G71800.1 32.000 75 51 0 35 109 11 85 1.18e-11 60.1
Msa0683940 AT1G60000.1 37.662 77 48 0 34 110 178 254 1.20e-11 59.7
Msa0683940 AT5G54900.1 39.474 76 45 1 35 109 156 231 1.31e-11 60.1
Msa0683940 AT4G34110.1 35.802 81 51 1 27 107 118 197 1.33e-11 60.1
Msa0683940 AT4G34110.1 37.500 80 50 0 32 111 35 114 9.91e-11 57.8
Msa0683940 AT4G13860.1 32.000 75 51 0 32 106 2 76 2.03e-11 55.8
Msa0683940 AT3G07810.2 38.158 76 45 1 34 109 7 80 2.04e-11 59.7
Msa0683940 AT3G07810.1 38.158 76 45 1 34 109 7 80 2.16e-11 59.7
Msa0683940 AT1G47490.2 38.158 76 46 1 35 109 199 274 2.37e-11 59.3
Msa0683940 AT4G00830.4 41.892 74 42 1 24 97 108 180 2.40e-11 59.3
Msa0683940 AT3G19130.1 38.158 76 46 1 35 109 204 279 3.03e-11 58.9
Msa0683940 AT4G24770.1 30.120 83 58 0 29 111 240 322 3.08e-11 58.9
Msa0683940 AT4G24770.2 30.120 83 58 0 29 111 240 322 3.08e-11 58.9
Msa0683940 AT1G49600.3 38.158 76 46 1 35 109 215 290 3.56e-11 58.9
Msa0683940 AT1G49600.2 38.158 76 46 1 35 109 215 290 3.56e-11 58.9
Msa0683940 AT1G47490.1 38.158 76 46 1 35 109 199 274 3.86e-11 58.9
Msa0683940 AT1G49600.1 38.158 76 46 1 35 109 215 290 4.00e-11 58.9
Msa0683940 AT3G54770.3 38.750 80 48 1 33 112 17 95 5.61e-11 57.8
Msa0683940 AT3G54770.1 38.462 78 47 1 26 103 10 86 6.51e-11 57.8
Msa0683940 AT5G53680.1 38.571 70 42 1 33 102 13 81 7.78e-11 56.2
Msa0683940 AT5G55550.5 34.211 76 48 1 34 109 7 80 9.69e-11 57.8
Msa0683940 AT5G55550.4 34.211 76 48 1 34 109 7 80 9.69e-11 57.8
Msa0683940 AT5G55550.9 34.211 76 48 1 34 109 7 80 9.69e-11 57.8
Msa0683940 AT5G55550.7 34.211 76 48 1 34 109 7 80 9.69e-11 57.8
Msa0683940 AT5G55550.1 34.211 76 48 1 34 109 7 80 9.69e-11 57.8

Find 47 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTACCGTGGCTATTAATT+TGG 0.181527 4_4:-47006464 None:intergenic
CTTATGTTTGTTTGGGCATT+TGG 0.190837 4_4:+47007581 Msa0683940:three_prime_UTR
AGCGGAAGTTTGAATTAATT+TGG 0.192087 4_4:+47007384 Msa0683940:three_prime_UTR
GACTGTTTCATTTGTGTTAT+AGG 0.273044 4_4:-47006670 None:intergenic
ACTTACTACTTATGTTTGTT+TGG 0.289779 4_4:+47007573 Msa0683940:three_prime_UTR
CTTACTACTTATGTTTGTTT+GGG 0.300099 4_4:+47007574 Msa0683940:three_prime_UTR
AATTTACGTTAATTTACTAT+TGG 0.302323 4_4:-47007478 None:intergenic
AATAGAGAACTTAGGTTTGT+TGG 0.319046 4_4:-47006389 None:intergenic
AACCGGTGATTAGTAGCATT+TGG 0.347306 4_4:+47007506 Msa0683940:three_prime_UTR
AAAACCTGCGATGAACAATT+TGG 0.369176 4_4:-47006539 None:intergenic
GTAATATGTGACCACAAAAC+TGG 0.373263 4_4:+47006810 Msa0683940:CDS
AAGTTTACTTATAACTTGTT+TGG 0.376472 4_4:+47007426 Msa0683940:three_prime_UTR
GTTATAAGTAAACTTTCATT+TGG 0.389139 4_4:-47007418 None:intergenic
TTAACGTAAATTTAAAGAAC+CGG 0.423966 4_4:+47007489 Msa0683940:three_prime_UTR
CACAAAACTGGAGAATCAAA+AGG 0.454249 4_4:+47006822 Msa0683940:CDS
TTTGAATTCGTTGTAGCTGC+TGG 0.454854 4_4:-47007315 None:intergenic
TACTTATAACTTGTTTGGAA+TGG 0.468858 4_4:+47007431 Msa0683940:three_prime_UTR
GTTGTTCGAAGGTGTCTCTC+AGG 0.483022 4_4:-47006692 None:intergenic
ACACTTGGAATAGAGAACTT+AGG 0.485636 4_4:-47006397 None:intergenic
CGCACGCATAGGAGCAATTG+AGG 0.496755 4_4:-47006502 None:intergenic
GTGCAATTCTTTGCGAGCTG+TGG 0.510560 4_4:-47006883 None:intergenic
TTCCAAATGCTACTAATCAC+CGG 0.528123 4_4:-47007508 None:intergenic
TCTACCAAATTGTTCATCGC+AGG 0.530928 4_4:+47006535 Msa0683940:CDS
ATGAATGATGGCGCACGCAT+AGG 0.535416 4_4:-47006513 None:intergenic
ATCATTTCTCAGATGGTGGA+TGG 0.549074 4_4:+47007224 Msa0683940:intron
GAGTGGGTTATGCGCACAAA+GGG 0.549317 4_4:+47007258 Msa0683940:CDS
ACCGTGGCTATTAATTTGGA+AGG 0.555706 4_4:-47006460 None:intergenic
TCCTTCCAAATTAATAGCCA+CGG 0.557857 4_4:+47006459 Msa0683940:CDS
TGTGAATTGCTTGAAGCACT+TGG 0.564171 4_4:-47007287 None:intergenic
TCGCAAAGAATTGCACGGCC+AGG 0.566715 4_4:+47006890 Msa0683940:CDS
ACTGGAGAATCAAAAGGGTA+CGG 0.577138 4_4:+47006828 Msa0683940:CDS
ACAAAACTGGAGAATCAAAA+GGG 0.577945 4_4:+47006823 Msa0683940:CDS
GATTTGCCCATGTTGTTCGA+AGG 0.586845 4_4:-47006703 None:intergenic
GTTTGGAATGGTGTTTACAA+TGG 0.597357 4_4:+47007443 Msa0683940:three_prime_UTR
AGAGACACCTTCGAACAACA+TGG 0.598698 4_4:+47006696 Msa0683940:CDS
ACAGCTCGCAAAGAATTGCA+CGG 0.599099 4_4:+47006885 Msa0683940:CDS
ACAATTTGGTAGATGAATGA+TGG 0.605999 4_4:-47006525 None:intergenic
CGAGTGGGTTATGCGCACAA+AGG 0.610846 4_4:+47007257 Msa0683940:CDS
AGCTCTACTATTTGCTACCG+TGG 0.618936 4_4:-47006476 None:intergenic
GGATGGTAGACGTATTCGAG+TGG 0.619447 4_4:+47007241 Msa0683940:CDS
CAACATGGGCAAATCATCGA+AGG 0.634641 4_4:+47006711 Msa0683940:CDS
CTTAGCTTGTAATCAACGAG+CGG 0.638669 4_4:+47007366 Msa0683940:three_prime_UTR
GATGGTAGACGTATTCGAGT+GGG 0.642189 4_4:+47007242 Msa0683940:CDS
CAAAAGGGTACGGATTTGTG+AGG 0.644341 4_4:+47006838 Msa0683940:CDS
AGTGTGAAGGTAGAAACACT+TGG 0.666934 4_4:-47006412 None:intergenic
AAACATACAGAACAGTGTGA+AGG 0.673028 4_4:-47006425 None:intergenic
GAGACACCTTCGAACAACAT+GGG 0.680329 4_4:+47006697 Msa0683940:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCTTTTCAAATTTAATATT+TGG + chr4_4:47006623-47006642 Msa0683940:intron 15.0%
!! AATTTACGTTAATTTACTAT+TGG - chr4_4:47007481-47007500 None:intergenic 15.0%
!!! AGCTTTTCAAATTTAATATT+TGG + chr4_4:47006623-47006642 Msa0683940:intron 15.0%
!! AATTTACGTTAATTTACTAT+TGG - chr4_4:47007481-47007500 None:intergenic 15.0%
!! AATAAAAACGAGAATGAAAT+GGG - chr4_4:47006577-47006596 None:intergenic 20.0%
!! AAATAAAAACGAGAATGAAA+TGG - chr4_4:47006578-47006597 None:intergenic 20.0%
!!! TTTAATATTTGGTTTTTGTC+TGG + chr4_4:47006634-47006653 Msa0683940:intron 20.0%
!! GTCTATAATATATTATAGAC+CGG + chr4_4:47007107-47007126 Msa0683940:intron 20.0%
!! AAATGATTAAGAACAGAAAA+AGG - chr4_4:47007211-47007230 None:intergenic 20.0%
!! GTTATAAGTAAACTTTCATT+TGG - chr4_4:47007421-47007440 None:intergenic 20.0%
!!! AAGTTTACTTATAACTTGTT+TGG + chr4_4:47007426-47007445 Msa0683940:three_prime_UTR 20.0%
!! TTAACGTAAATTTAAAGAAC+CGG + chr4_4:47007489-47007508 Msa0683940:three_prime_UTR 20.0%
!! AATAAAAACGAGAATGAAAT+GGG - chr4_4:47006577-47006596 None:intergenic 20.0%
!! AAATAAAAACGAGAATGAAA+TGG - chr4_4:47006578-47006597 None:intergenic 20.0%
!!! TTTAATATTTGGTTTTTGTC+TGG + chr4_4:47006634-47006653 Msa0683940:intron 20.0%
!! GTCTATAATATATTATAGAC+CGG + chr4_4:47007107-47007126 Msa0683940:intron 20.0%
!! AAATGATTAAGAACAGAAAA+AGG - chr4_4:47007211-47007230 None:intergenic 20.0%
!! GTTATAAGTAAACTTTCATT+TGG - chr4_4:47007421-47007440 None:intergenic 20.0%
!!! AAGTTTACTTATAACTTGTT+TGG + chr4_4:47007426-47007445 Msa0683940:three_prime_UTR 20.0%
!! TTAACGTAAATTTAAAGAAC+CGG + chr4_4:47007489-47007508 Msa0683940:three_prime_UTR 20.0%
!!! CATTTTTAAAAGTTACTCTC+CGG - chr4_4:47007129-47007148 None:intergenic 25.0%
!!! GAGTAACTTTTAAAAATGCA+TGG + chr4_4:47007131-47007150 Msa0683940:intron 25.0%
! AGAAAAAGGTTCATAACATT+AGG - chr4_4:47007197-47007216 None:intergenic 25.0%
!!! TACTTATAACTTGTTTGGAA+TGG + chr4_4:47007431-47007450 Msa0683940:three_prime_UTR 25.0%
! ACTTACTACTTATGTTTGTT+TGG + chr4_4:47007573-47007592 Msa0683940:three_prime_UTR 25.0%
! CTTACTACTTATGTTTGTTT+GGG + chr4_4:47007574-47007593 Msa0683940:three_prime_UTR 25.0%
!!! CATTTTTAAAAGTTACTCTC+CGG - chr4_4:47007129-47007148 None:intergenic 25.0%
!!! GAGTAACTTTTAAAAATGCA+TGG + chr4_4:47007131-47007150 Msa0683940:intron 25.0%
! AGAAAAAGGTTCATAACATT+AGG - chr4_4:47007197-47007216 None:intergenic 25.0%
!!! TACTTATAACTTGTTTGGAA+TGG + chr4_4:47007431-47007450 Msa0683940:three_prime_UTR 25.0%
! ACTTACTACTTATGTTTGTT+TGG + chr4_4:47007573-47007592 Msa0683940:three_prime_UTR 25.0%
! CTTACTACTTATGTTTGTTT+GGG + chr4_4:47007574-47007593 Msa0683940:three_prime_UTR 25.0%
AATAGAGAACTTAGGTTTGT+TGG - chr4_4:47006392-47006411 None:intergenic 30.0%
ACAATTTGGTAGATGAATGA+TGG - chr4_4:47006528-47006547 None:intergenic 30.0%
GACTGTTTCATTTGTGTTAT+AGG - chr4_4:47006673-47006692 None:intergenic 30.0%
ATGTTAATAAGATCCCCAAA+TGG + chr4_4:47006749-47006768 Msa0683940:intron 30.0%
TGTTAATAAGATCCCCAAAT+GGG + chr4_4:47006750-47006769 Msa0683940:intron 30.0%
ACAAAACTGGAGAATCAAAA+GGG + chr4_4:47006823-47006842 Msa0683940:CDS 30.0%
AATATGAAATCTCCATTACC+TGG - chr4_4:47006911-47006930 None:intergenic 30.0%
TCTGTCTCTTGAAGTTATAA+AGG + chr4_4:47006987-47007006 Msa0683940:intron 30.0%
GTTCTTAATCATTTCTCAGA+TGG + chr4_4:47007217-47007236 Msa0683940:intron 30.0%
AGCGGAAGTTTGAATTAATT+TGG + chr4_4:47007384-47007403 Msa0683940:three_prime_UTR 30.0%
!!! TAAGTGACTGTTTGTTTTAG+CGG - chr4_4:47007558-47007577 None:intergenic 30.0%
AATAGAGAACTTAGGTTTGT+TGG - chr4_4:47006392-47006411 None:intergenic 30.0%
ACAATTTGGTAGATGAATGA+TGG - chr4_4:47006528-47006547 None:intergenic 30.0%
GACTGTTTCATTTGTGTTAT+AGG - chr4_4:47006673-47006692 None:intergenic 30.0%
ATGTTAATAAGATCCCCAAA+TGG + chr4_4:47006749-47006768 Msa0683940:intron 30.0%
TGTTAATAAGATCCCCAAAT+GGG + chr4_4:47006750-47006769 Msa0683940:intron 30.0%
ACAAAACTGGAGAATCAAAA+GGG + chr4_4:47006823-47006842 Msa0683940:CDS 30.0%
AATATGAAATCTCCATTACC+TGG - chr4_4:47006911-47006930 None:intergenic 30.0%
TCTGTCTCTTGAAGTTATAA+AGG + chr4_4:47006987-47007006 Msa0683940:intron 30.0%
GTTCTTAATCATTTCTCAGA+TGG + chr4_4:47007217-47007236 Msa0683940:intron 30.0%
AGCGGAAGTTTGAATTAATT+TGG + chr4_4:47007384-47007403 Msa0683940:three_prime_UTR 30.0%
!!! TAAGTGACTGTTTGTTTTAG+CGG - chr4_4:47007558-47007577 None:intergenic 30.0%
ACACTTGGAATAGAGAACTT+AGG - chr4_4:47006400-47006419 None:intergenic 35.0%
AAACATACAGAACAGTGTGA+AGG - chr4_4:47006428-47006447 None:intergenic 35.0%
TCCTTCCAAATTAATAGCCA+CGG + chr4_4:47006459-47006478 Msa0683940:CDS 35.0%
AAAACCTGCGATGAACAATT+TGG - chr4_4:47006542-47006561 None:intergenic 35.0%
AAAACGAGAATGAAATGGGT+TGG - chr4_4:47006573-47006592 None:intergenic 35.0%
!! TTGGTTTTTGTCTGGAATGA+AGG + chr4_4:47006642-47006661 Msa0683940:intron 35.0%
!! TGGTTTTTGTCTGGAATGAA+GGG + chr4_4:47006643-47006662 Msa0683940:intron 35.0%
AGTTCCGAAATAACCCATTT+GGG - chr4_4:47006766-47006785 None:intergenic 35.0%
TAGTTCCGAAATAACCCATT+TGG - chr4_4:47006767-47006786 None:intergenic 35.0%
GTAATATGTGACCACAAAAC+TGG + chr4_4:47006810-47006829 Msa0683940:CDS 35.0%
!! CTTTTGATTCTCCAGTTTTG+TGG - chr4_4:47006824-47006843 None:intergenic 35.0%
CACAAAACTGGAGAATCAAA+AGG + chr4_4:47006822-47006841 Msa0683940:CDS 35.0%
! ATGACCATTTTACAATCCAG+AGG + chr4_4:47006957-47006976 Msa0683940:intron 35.0%
CTTAATCATTTCTCAGATGG+TGG + chr4_4:47007220-47007239 Msa0683940:intron 35.0%
! GTTTGGAATGGTGTTTACAA+TGG + chr4_4:47007443-47007462 Msa0683940:three_prime_UTR 35.0%
TTCCAAATGCTACTAATCAC+CGG - chr4_4:47007511-47007530 None:intergenic 35.0%
CTTATGTTTGTTTGGGCATT+TGG + chr4_4:47007581-47007600 Msa0683940:three_prime_UTR 35.0%
ACACTTGGAATAGAGAACTT+AGG - chr4_4:47006400-47006419 None:intergenic 35.0%
AAACATACAGAACAGTGTGA+AGG - chr4_4:47006428-47006447 None:intergenic 35.0%
TCCTTCCAAATTAATAGCCA+CGG + chr4_4:47006459-47006478 Msa0683940:CDS 35.0%
AAAACCTGCGATGAACAATT+TGG - chr4_4:47006542-47006561 None:intergenic 35.0%
AAAACGAGAATGAAATGGGT+TGG - chr4_4:47006573-47006592 None:intergenic 35.0%
!! TTGGTTTTTGTCTGGAATGA+AGG + chr4_4:47006642-47006661 Msa0683940:intron 35.0%
!! TGGTTTTTGTCTGGAATGAA+GGG + chr4_4:47006643-47006662 Msa0683940:intron 35.0%
AGTTCCGAAATAACCCATTT+GGG - chr4_4:47006766-47006785 None:intergenic 35.0%
TAGTTCCGAAATAACCCATT+TGG - chr4_4:47006767-47006786 None:intergenic 35.0%
GTAATATGTGACCACAAAAC+TGG + chr4_4:47006810-47006829 Msa0683940:CDS 35.0%
!! CTTTTGATTCTCCAGTTTTG+TGG - chr4_4:47006824-47006843 None:intergenic 35.0%
CACAAAACTGGAGAATCAAA+AGG + chr4_4:47006822-47006841 Msa0683940:CDS 35.0%
! ATGACCATTTTACAATCCAG+AGG + chr4_4:47006957-47006976 Msa0683940:intron 35.0%
CTTAATCATTTCTCAGATGG+TGG + chr4_4:47007220-47007239 Msa0683940:intron 35.0%
! GTTTGGAATGGTGTTTACAA+TGG + chr4_4:47007443-47007462 Msa0683940:three_prime_UTR 35.0%
TTCCAAATGCTACTAATCAC+CGG - chr4_4:47007511-47007530 None:intergenic 35.0%
CTTATGTTTGTTTGGGCATT+TGG + chr4_4:47007581-47007600 Msa0683940:three_prime_UTR 35.0%
AGTGTGAAGGTAGAAACACT+TGG - chr4_4:47006415-47006434 None:intergenic 40.0%
! ACCGTGGCTATTAATTTGGA+AGG - chr4_4:47006463-47006482 None:intergenic 40.0%
TGCTACCGTGGCTATTAATT+TGG - chr4_4:47006467-47006486 None:intergenic 40.0%
TCTACCAAATTGTTCATCGC+AGG + chr4_4:47006535-47006554 Msa0683940:CDS 40.0%
!! AGAATGAAATGGGTTGGAGA+AGG - chr4_4:47006567-47006586 None:intergenic 40.0%
!! GATCCCCAAATGGGTTATTT+CGG + chr4_4:47006759-47006778 Msa0683940:intron 40.0%
GTTCCGAAATAACCCATTTG+GGG - chr4_4:47006765-47006784 None:intergenic 40.0%
ACTGGAGAATCAAAAGGGTA+CGG + chr4_4:47006828-47006847 Msa0683940:CDS 40.0%
CGAACCTCTGGATTGTAAAA+TGG - chr4_4:47006964-47006983 None:intergenic 40.0%
ACTTCAAGTGTTAGCACACA+GGG + chr4_4:47007020-47007039 Msa0683940:intron 40.0%
!! ATCATTTCTCAGATGGTGGA+TGG + chr4_4:47007224-47007243 Msa0683940:intron 40.0%
! TGTGAATTGCTTGAAGCACT+TGG - chr4_4:47007290-47007309 None:intergenic 40.0%
TTTGAATTCGTTGTAGCTGC+TGG - chr4_4:47007318-47007337 None:intergenic 40.0%
!! CTTAGCTTGTAATCAACGAG+CGG + chr4_4:47007366-47007385 Msa0683940:three_prime_UTR 40.0%
AACCGGTGATTAGTAGCATT+TGG + chr4_4:47007506-47007525 Msa0683940:three_prime_UTR 40.0%
AGTGTGAAGGTAGAAACACT+TGG - chr4_4:47006415-47006434 None:intergenic 40.0%
! ACCGTGGCTATTAATTTGGA+AGG - chr4_4:47006463-47006482 None:intergenic 40.0%
TGCTACCGTGGCTATTAATT+TGG - chr4_4:47006467-47006486 None:intergenic 40.0%
TCTACCAAATTGTTCATCGC+AGG + chr4_4:47006535-47006554 Msa0683940:CDS 40.0%
!! AGAATGAAATGGGTTGGAGA+AGG - chr4_4:47006567-47006586 None:intergenic 40.0%
!! GATCCCCAAATGGGTTATTT+CGG + chr4_4:47006759-47006778 Msa0683940:intron 40.0%
GTTCCGAAATAACCCATTTG+GGG - chr4_4:47006765-47006784 None:intergenic 40.0%
ACTGGAGAATCAAAAGGGTA+CGG + chr4_4:47006828-47006847 Msa0683940:CDS 40.0%
CGAACCTCTGGATTGTAAAA+TGG - chr4_4:47006964-47006983 None:intergenic 40.0%
ACTTCAAGTGTTAGCACACA+GGG + chr4_4:47007020-47007039 Msa0683940:intron 40.0%
!! ATCATTTCTCAGATGGTGGA+TGG + chr4_4:47007224-47007243 Msa0683940:intron 40.0%
! TGTGAATTGCTTGAAGCACT+TGG - chr4_4:47007290-47007309 None:intergenic 40.0%
TTTGAATTCGTTGTAGCTGC+TGG - chr4_4:47007318-47007337 None:intergenic 40.0%
!! CTTAGCTTGTAATCAACGAG+CGG + chr4_4:47007366-47007385 Msa0683940:three_prime_UTR 40.0%
AACCGGTGATTAGTAGCATT+TGG + chr4_4:47007506-47007525 Msa0683940:three_prime_UTR 40.0%
! AGCTCTACTATTTGCTACCG+TGG - chr4_4:47006479-47006498 None:intergenic 45.0%
AGAGACACCTTCGAACAACA+TGG + chr4_4:47006696-47006715 Msa0683940:CDS 45.0%
GAGACACCTTCGAACAACAT+GGG + chr4_4:47006697-47006716 Msa0683940:CDS 45.0%
GATTTGCCCATGTTGTTCGA+AGG - chr4_4:47006706-47006725 None:intergenic 45.0%
CAACATGGGCAAATCATCGA+AGG + chr4_4:47006711-47006730 Msa0683940:CDS 45.0%
! CAAAAGGGTACGGATTTGTG+AGG + chr4_4:47006838-47006857 Msa0683940:CDS 45.0%
ACAGCTCGCAAAGAATTGCA+CGG + chr4_4:47006885-47006904 Msa0683940:CDS 45.0%
CACTTCAAGTGTTAGCACAC+AGG + chr4_4:47007019-47007038 Msa0683940:intron 45.0%
GATGGTAGACGTATTCGAGT+GGG + chr4_4:47007242-47007261 Msa0683940:CDS 45.0%
! AGCTCTACTATTTGCTACCG+TGG - chr4_4:47006479-47006498 None:intergenic 45.0%
AGAGACACCTTCGAACAACA+TGG + chr4_4:47006696-47006715 Msa0683940:CDS 45.0%
GAGACACCTTCGAACAACAT+GGG + chr4_4:47006697-47006716 Msa0683940:CDS 45.0%
GATTTGCCCATGTTGTTCGA+AGG - chr4_4:47006706-47006725 None:intergenic 45.0%
CAACATGGGCAAATCATCGA+AGG + chr4_4:47006711-47006730 Msa0683940:CDS 45.0%
! CAAAAGGGTACGGATTTGTG+AGG + chr4_4:47006838-47006857 Msa0683940:CDS 45.0%
ACAGCTCGCAAAGAATTGCA+CGG + chr4_4:47006885-47006904 Msa0683940:CDS 45.0%
CACTTCAAGTGTTAGCACAC+AGG + chr4_4:47007019-47007038 Msa0683940:intron 45.0%
GATGGTAGACGTATTCGAGT+GGG + chr4_4:47007242-47007261 Msa0683940:CDS 45.0%
! ATGAATGATGGCGCACGCAT+AGG - chr4_4:47006516-47006535 None:intergenic 50.0%
!! GTTGTTCGAAGGTGTCTCTC+AGG - chr4_4:47006695-47006714 None:intergenic 50.0%
GTGCAATTCTTTGCGAGCTG+TGG - chr4_4:47006886-47006905 None:intergenic 50.0%
AGAATTGCACGGCCAGGTAA+TGG + chr4_4:47006896-47006915 Msa0683940:intron 50.0%
AGAGACAGAGTTCGAACCTC+TGG - chr4_4:47006976-47006995 None:intergenic 50.0%
GGATGGTAGACGTATTCGAG+TGG + chr4_4:47007241-47007260 Msa0683940:CDS 50.0%
!! GAGTGGGTTATGCGCACAAA+GGG + chr4_4:47007258-47007277 Msa0683940:CDS 50.0%
! ATGAATGATGGCGCACGCAT+AGG - chr4_4:47006516-47006535 None:intergenic 50.0%
!! GTTGTTCGAAGGTGTCTCTC+AGG - chr4_4:47006695-47006714 None:intergenic 50.0%
GTGCAATTCTTTGCGAGCTG+TGG - chr4_4:47006886-47006905 None:intergenic 50.0%
AGAATTGCACGGCCAGGTAA+TGG + chr4_4:47006896-47006915 Msa0683940:intron 50.0%
AGAGACAGAGTTCGAACCTC+TGG - chr4_4:47006976-47006995 None:intergenic 50.0%
GGATGGTAGACGTATTCGAG+TGG + chr4_4:47007241-47007260 Msa0683940:CDS 50.0%
!! GAGTGGGTTATGCGCACAAA+GGG + chr4_4:47007258-47007277 Msa0683940:CDS 50.0%
CGCACGCATAGGAGCAATTG+AGG - chr4_4:47006505-47006524 None:intergenic 55.0%
TCGCAAAGAATTGCACGGCC+AGG + chr4_4:47006890-47006909 Msa0683940:CDS 55.0%
!! CGAGTGGGTTATGCGCACAA+AGG + chr4_4:47007257-47007276 Msa0683940:CDS 55.0%
CGCACGCATAGGAGCAATTG+AGG - chr4_4:47006505-47006524 None:intergenic 55.0%
TCGCAAAGAATTGCACGGCC+AGG + chr4_4:47006890-47006909 Msa0683940:CDS 55.0%
!! CGAGTGGGTTATGCGCACAA+AGG + chr4_4:47007257-47007276 Msa0683940:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 47006366 47007635 47006366 ID=Msa0683940;Name=Msa0683940
chr4_4 mRNA 47006366 47007635 47006366 ID=Msa0683940-mRNA-1;Parent=Msa0683940;Name=Msa0683940-mRNA-1;_AED=0.10;_eAED=0.10;_QI=76|1|1|1|1|1|4|352|112
chr4_4 exon 47006366 47006556 47006366 ID=Msa0683940-mRNA-1:exon:11567;Parent=Msa0683940-mRNA-1
chr4_4 exon 47006664 47006732 47006664 ID=Msa0683940-mRNA-1:exon:11568;Parent=Msa0683940-mRNA-1
chr4_4 exon 47006805 47006911 47006805 ID=Msa0683940-mRNA-1:exon:11569;Parent=Msa0683940-mRNA-1
chr4_4 exon 47007236 47007635 47007236 ID=Msa0683940-mRNA-1:exon:11570;Parent=Msa0683940-mRNA-1
chr4_4 five_prime_UTR 47006366 47006441 47006366 ID=Msa0683940-mRNA-1:five_prime_utr;Parent=Msa0683940-mRNA-1
chr4_4 CDS 47006442 47006556 47006442 ID=Msa0683940-mRNA-1:cds;Parent=Msa0683940-mRNA-1
chr4_4 CDS 47006664 47006732 47006664 ID=Msa0683940-mRNA-1:cds;Parent=Msa0683940-mRNA-1
chr4_4 CDS 47006805 47006911 47006805 ID=Msa0683940-mRNA-1:cds;Parent=Msa0683940-mRNA-1
chr4_4 CDS 47007236 47007283 47007236 ID=Msa0683940-mRNA-1:cds;Parent=Msa0683940-mRNA-1
chr4_4 three_prime_UTR 47007284 47007635 47007284 ID=Msa0683940-mRNA-1:three_prime_utr;Parent=Msa0683940-mRNA-1
Gene Sequence

>Msa0683940

ATGTTTGTTCATCTTACTCCTTCCAAATTAATAGCCACGGTAGCAAATAGTAGAGCTCTTCCTCAATTGCTCCTATGCGTGCGCCATCATTCATCTACCAAATTGTTCATCGCAGGGCTTTCCTATAACACAAATGAAACAGTCCTGAGAGACACCTTCGAACAACATGGGCAAATCATCGAAGTTAAAGTAATATGTGACCACAAAACTGGAGAATCAAAAGGGTACGGATTTGTGAGGTTCAATTCTGAAACTGCAGCTGCCACAGCTCGCAAAGAATTGCACGGCCAGATGGTGGATGGTAGACGTATTCGAGTGGGTTATGCGCACAAAGGGTAA
Protein sequence

>Msa0683940

MFVHLTPSKLIATVANSRALPQLLLCVRHHSSTKLFIAGLSYNTNETVLRDTFEQHGQIIEVKVICDHKTGESKGYGFVRFNSETAAATARKELHGQMVDGRRIRVGYAHKG*