AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0701990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0701990 MtrunA17_Chr8g0380011 94.054 185 10 1 1 185 1 184 1.39e-130 363
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0701990 AT4G18930.1 56.497 177 77 0 8 184 5 181 3.26e-73 219
Msa0701990 AT4G18940.1 46.328 177 90 2 8 182 21 194 1.22e-53 169

Find 84 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCCACCCCTCAGATTC+TGG 0.205221 4_4:-73442935 Msa0701990:CDS
TGAAGACCGAAGAGGGAATA+TGG 0.228778 4_4:-73443339 Msa0701990:five_prime_UTR
CGGTCATGTGGGGTTCGAAC+TGG 0.275943 4_4:+73443097 None:intergenic
AACAAAGAAGGAGGTTTATT+CGG 0.336401 4_4:-73443199 Msa0701990:CDS
TTTCCTCATCATGTCATCTA+TGG 0.336553 4_4:+73441465 None:intergenic
GAACTGGGGTCCACCGAATT+CGG 0.348912 4_4:+73443113 None:intergenic
AAGACAAATCTCTTAAATCT+TGG 0.349286 4_4:-73441758 Msa0701990:CDS
TTGAATTGTTATAACCGAAA+TGG 0.353367 4_4:+73442890 None:intergenic
GGTCATGTGGGGTTCGAACT+GGG 0.353795 4_4:+73443098 None:intergenic
GTGTTGTTGGCAAGCTAGTT+TGG 0.355529 4_4:+73441708 None:intergenic
AGTTTGTTGAGTGCATCGTC+TGG 0.357178 4_4:+73443051 None:intergenic
CTTCTTGTTGTCACAATCAA+AGG 0.371336 4_4:+73443260 None:intergenic
GCACACTGCTGCACCCATTT+CGG 0.374682 4_4:-73442904 Msa0701990:CDS
AGGCGGTCGCATACGTCTTC+CGG 0.404707 4_4:+73443162 None:intergenic
ACCGCCCTCCGATCCGAATT+CGG 0.405972 4_4:-73443126 Msa0701990:CDS
TGAATTGTTATAACCGAAAT+GGG 0.412619 4_4:+73442891 None:intergenic
AGACATACAGACAATATTAG+AGG 0.431901 4_4:+73441624 None:intergenic
AGACAAATCTCTTAAATCTT+GGG 0.434674 4_4:-73441757 Msa0701990:CDS
GTTTGTTGAGTGCATCGTCT+GGG 0.440660 4_4:+73443052 None:intergenic
ATATTCCCATATTCCCTCTT+CGG 0.442010 4_4:+73443333 None:intergenic
CAACACGGTCAACGGTGACT+TGG 0.470908 4_4:+73442998 None:intergenic
TGTGATGGTATAAGTGTTGT+TGG 0.481063 4_4:+73441695 None:intergenic
TGCTCCCACGACGGTCATGT+GGG 0.482469 4_4:+73443086 None:intergenic
TCTGAGGGGTGGGATGAAGA+AGG 0.483134 4_4:+73442941 None:intergenic
TTCTTGTTGTCACAATCAAA+GGG 0.494787 4_4:+73443261 None:intergenic
TTGCTCCCACGACGGTCATG+TGG 0.500012 4_4:+73443085 None:intergenic
GAAGACCGAAGAGGGAATAT+GGG 0.501026 4_4:-73443338 Msa0701990:five_prime_UTR
TTCGGTGTGGGCGATCCCAC+CGG 0.504778 4_4:-73443181 Msa0701990:CDS
CAACAAGAAGGAGAGGTGAT+TGG 0.523497 4_4:-73443247 Msa0701990:five_prime_UTR
GAGTGATGAAGAGAAGCAAA+AGG 0.529008 4_4:-73441868 Msa0701990:CDS
TCGGAGGGCGGTCATGAGCT+TGG 0.530142 4_4:+73443137 None:intergenic
CAGAATATAAATAGAATACA+AGG 0.530972 4_4:+73441575 None:intergenic
CCACACGTGAGCCTTCTGTA+TGG 0.533433 4_4:-73441897 Msa0701990:CDS
GTCCACCGAATTCGGATCGG+AGG 0.536297 4_4:+73443121 None:intergenic
AACATCATCAACAACAAAGA+AGG 0.536359 4_4:-73443211 Msa0701990:CDS
ACCGTTGACCGTGTTGCCGC+CGG 0.541024 4_4:-73442991 Msa0701990:CDS
TTGTTGATGATGTTGCCATT+CGG 0.544074 4_4:+73443220 None:intergenic
CATTGGTTTCCAGAATCTGA+GGG 0.544773 4_4:+73442926 None:intergenic
GAGGGAATATGGGAATATGC+GGG 0.544843 4_4:-73443328 Msa0701990:five_prime_UTR
GTTTCCAGAATCTGAGGGGT+GGG 0.544896 4_4:+73442931 None:intergenic
GCATTGGTTTCCAGAATCTG+AGG 0.548716 4_4:+73442925 None:intergenic
GAAGGAGGTTTATTCGGTGT+GGG 0.549645 4_4:-73443193 Msa0701990:CDS
GTCATGTGGGGTTCGAACTG+GGG 0.558588 4_4:+73443099 None:intergenic
GCTTACTTATCTGAAAGCTC+AGG 0.559929 4_4:+73441805 None:intergenic
CGGTCGCATACGTCTTCCGG+TGG 0.566606 4_4:+73443165 None:intergenic
CTTTGATTGTGACAACAAGA+AGG 0.568699 4_4:-73443259 Msa0701990:five_prime_UTR
ATAAACACACTGATAAAAGA+AGG 0.569569 4_4:+73442966 None:intergenic
GCCGGCGGCAACACGGTCAA+CGG 0.570666 4_4:+73442990 None:intergenic
AGAAGGAGGTTTATTCGGTG+TGG 0.570758 4_4:-73443194 Msa0701990:CDS
TCCACCGAATTCGGATCGGA+GGG 0.574564 4_4:+73443122 None:intergenic
ATAAATAGAATACAAGGCAG+TGG 0.578525 4_4:+73441581 None:intergenic
CACTCAAATCTCCATACAGA+AGG 0.585673 4_4:+73441886 None:intergenic
CGGTCATGAGCTTGGTGAGG+CGG 0.586534 4_4:+73443145 None:intergenic
ATGGGAATATGCGGGGCATG+TGG 0.587433 4_4:-73443320 Msa0701990:five_prime_UTR
GGGTGAGTGAAGACCGAAGA+GGG 0.588478 4_4:-73443346 Msa0701990:five_prime_UTR
ATGGGTGCAGCAGTGTGCAT+TGG 0.592009 4_4:+73442909 None:intergenic
GGTTTCCAGAATCTGAGGGG+TGG 0.595287 4_4:+73442930 None:intergenic
TATGCGGGGCATGTGGCACA+TGG 0.598398 4_4:-73443313 Msa0701990:five_prime_UTR
ACCGAATTCGGATCGGAGGG+CGG 0.604262 4_4:+73443125 None:intergenic
TTTGTTGAGTGCATCGTCTG+GGG 0.606981 4_4:+73443053 None:intergenic
GAGGTGATTGGTACTCCGAA+TGG 0.608085 4_4:-73443235 Msa0701990:exon
AAACTCCGATCAGCTTCTGA+AGG 0.608276 4_4:-73443033 Msa0701990:CDS
AGAAGGTGCCGGCGGCAACA+CGG 0.609371 4_4:+73442983 None:intergenic
TAACACCTTCAGAAGCTGAT+CGG 0.616481 4_4:+73443028 None:intergenic
GGGCGGTCATGAGCTTGGTG+AGG 0.619509 4_4:+73443142 None:intergenic
GCCCTCCGATCCGAATTCGG+TGG 0.623631 4_4:-73443123 Msa0701990:CDS
GGTCGCATACGTCTTCCGGT+GGG 0.624642 4_4:+73443166 None:intergenic
ATTGTGACAACAAGAAGGAG+AGG 0.625534 4_4:-73443254 Msa0701990:five_prime_UTR
AGAGGGAATATGGGAATATG+CGG 0.627630 4_4:-73443329 Msa0701990:five_prime_UTR
GGGGTCCACCGAATTCGGAT+CGG 0.635044 4_4:+73443118 None:intergenic
CAACAGTTGTGAAGTTGTGA+TGG 0.641809 4_4:+73441680 None:intergenic
ATCATCAACAACAAAGAAGG+AGG 0.646539 4_4:-73443208 Msa0701990:CDS
GCTCCCACGACGGTCATGTG+GGG 0.646603 4_4:+73443087 None:intergenic
GAACCCCACATGACCGTCGT+GGG 0.656303 4_4:-73443090 Msa0701990:CDS
CGAACCCCACATGACCGTCG+TGG 0.660724 4_4:-73443091 Msa0701990:CDS
CCATACAGAAGGCTCACGTG+TGG 0.675755 4_4:+73441897 None:intergenic
AGGGAATATGGGAATATGCG+GGG 0.678830 4_4:-73443327 Msa0701990:five_prime_UTR
CTGATAAAAGAAGGTGCCGG+CGG 0.680708 4_4:+73442975 None:intergenic
ATTCTTGACGACAGTCTCAG+TGG 0.687938 4_4:-73441828 Msa0701990:CDS
ATTGGTTTCCAGAATCTGAG+GGG 0.690122 4_4:+73442927 None:intergenic
ACACTGATAAAAGAAGGTGC+CGG 0.691010 4_4:+73442972 None:intergenic
TAACTCTATTGCTCCCACGA+CGG 0.696694 4_4:+73443077 None:intergenic
GTGCCATAGATGACATGATG+AGG 0.710145 4_4:-73441468 Msa0701990:three_prime_UTR
TGGGTGAGTGAAGACCGAAG+AGG 0.751231 4_4:-73443347 Msa0701990:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAGTTTTTTTTTTACTTTT+TGG - chr4_4:73442793-73442812 Msa0701990:intron 15.0%
!!! TGAGTTTTTTTTTTACTTTT+TGG - chr4_4:73442793-73442812 Msa0701990:intron 15.0%
!!! ATAGAAAACGTATATTTTGA+TGG - chr4_4:73441992-73442011 Msa0701990:intron 20.0%
!!! TAGAAAACGTATATTTTGAT+GGG - chr4_4:73441993-73442012 Msa0701990:intron 20.0%
!! CAGAATATAAATAGAATACA+AGG + chr4_4:73443231-73443250 None:intergenic 20.0%
!!! ATAGAAAACGTATATTTTGA+TGG - chr4_4:73441992-73442011 Msa0701990:intron 20.0%
!!! TAGAAAACGTATATTTTGAT+GGG - chr4_4:73441993-73442012 Msa0701990:intron 20.0%
!! CAGAATATAAATAGAATACA+AGG + chr4_4:73443231-73443250 None:intergenic 20.0%
! ATAAACACACTGATAAAAGA+AGG + chr4_4:73441840-73441859 None:intergenic 25.0%
! TGAATTGTTATAACCGAAAT+GGG + chr4_4:73441915-73441934 None:intergenic 25.0%
! TTGAATTGTTATAACCGAAA+TGG + chr4_4:73441916-73441935 None:intergenic 25.0%
! TGAGATTTGAAAATCTCATT+AGG + chr4_4:73442240-73442259 None:intergenic 25.0%
!! AATGAGATTTTCAAATCTCA+AGG - chr4_4:73442240-73442259 Msa0701990:intron 25.0%
!! ATGAGATTTTCAAATCTCAA+GGG - chr4_4:73442241-73442260 Msa0701990:intron 25.0%
!!! TTTTTTTGATTGATTCACCA+GGG + chr4_4:73442375-73442394 None:intergenic 25.0%
! CTTGGAACATAAATTGTTAT+CGG - chr4_4:73442556-73442575 Msa0701990:intron 25.0%
! TTGGAACATAAATTGTTATC+GGG - chr4_4:73442557-73442576 Msa0701990:intron 25.0%
! ATATATCTATCTTCCTTCAT+TGG + chr4_4:73442595-73442614 None:intergenic 25.0%
! AATTATGTAGCAATAGCATT+AGG - chr4_4:73442708-73442727 Msa0701990:intron 25.0%
! ATTATGTAGCAATAGCATTA+GGG - chr4_4:73442709-73442728 Msa0701990:intron 25.0%
! CATTAAAACAATGTACACAT+TGG + chr4_4:73442873-73442892 None:intergenic 25.0%
! AAGACAAATCTCTTAAATCT+TGG - chr4_4:73443045-73443064 Msa0701990:CDS 25.0%
! AGACAAATCTCTTAAATCTT+GGG - chr4_4:73443046-73443065 Msa0701990:CDS 25.0%
! AGTCACATAAAAACATTACT+TGG + chr4_4:73443300-73443319 None:intergenic 25.0%
! ATAAACACACTGATAAAAGA+AGG + chr4_4:73441840-73441859 None:intergenic 25.0%
! TGAATTGTTATAACCGAAAT+GGG + chr4_4:73441915-73441934 None:intergenic 25.0%
! TTGAATTGTTATAACCGAAA+TGG + chr4_4:73441916-73441935 None:intergenic 25.0%
! TGAGATTTGAAAATCTCATT+AGG + chr4_4:73442240-73442259 None:intergenic 25.0%
!! AATGAGATTTTCAAATCTCA+AGG - chr4_4:73442240-73442259 Msa0701990:intron 25.0%
!! ATGAGATTTTCAAATCTCAA+GGG - chr4_4:73442241-73442260 Msa0701990:intron 25.0%
!!! TTTTTTTGATTGATTCACCA+GGG + chr4_4:73442375-73442394 None:intergenic 25.0%
! CTTGGAACATAAATTGTTAT+CGG - chr4_4:73442556-73442575 Msa0701990:intron 25.0%
! TTGGAACATAAATTGTTATC+GGG - chr4_4:73442557-73442576 Msa0701990:intron 25.0%
! ATATATCTATCTTCCTTCAT+TGG + chr4_4:73442595-73442614 None:intergenic 25.0%
! AATTATGTAGCAATAGCATT+AGG - chr4_4:73442708-73442727 Msa0701990:intron 25.0%
! ATTATGTAGCAATAGCATTA+GGG - chr4_4:73442709-73442728 Msa0701990:intron 25.0%
! CATTAAAACAATGTACACAT+TGG + chr4_4:73442873-73442892 None:intergenic 25.0%
! AAGACAAATCTCTTAAATCT+TGG - chr4_4:73443045-73443064 Msa0701990:CDS 25.0%
! AGACAAATCTCTTAAATCTT+GGG - chr4_4:73443046-73443065 Msa0701990:CDS 25.0%
! AGTCACATAAAAACATTACT+TGG + chr4_4:73443300-73443319 None:intergenic 25.0%
! TGGACAATTGACATGTTTTA+AGG - chr4_4:73441510-73441529 Msa0701990:three_prime_UTR 30.0%
!! GGACAATTGACATGTTTTAA+GGG - chr4_4:73441511-73441530 Msa0701990:three_prime_UTR 30.0%
! TTCTTGTTGTCACAATCAAA+GGG + chr4_4:73441545-73441564 None:intergenic 30.0%
AACATCATCAACAACAAAGA+AGG - chr4_4:73441592-73441611 Msa0701990:three_prime_UTR 30.0%
AACAAAGAAGGAGGTTTATT+CGG - chr4_4:73441604-73441623 Msa0701990:three_prime_UTR 30.0%
!!! AAAACGTATATTTTGATGGG+TGG - chr4_4:73441996-73442015 Msa0701990:intron 30.0%
!!! AAACGTATATTTTGATGGGT+GGG - chr4_4:73441997-73442016 Msa0701990:intron 30.0%
TCATTCAGGTATATTGTGAT+CGG + chr4_4:73442067-73442086 None:intergenic 30.0%
TAATCAAGTAGCAAAGAGTT+TGG - chr4_4:73442118-73442137 Msa0701990:intron 30.0%
!! TTCATATCCTTGGATTAGTT+GGG - chr4_4:73442208-73442227 Msa0701990:intron 30.0%
! AATCTGCAACTTGTTCTTTA+AGG + chr4_4:73442284-73442303 None:intergenic 30.0%
TCATTGCGATTGAATTCATA+TGG - chr4_4:73442346-73442365 Msa0701990:intron 30.0%
!!! GTTTTTTTGATTGATTCACC+AGG + chr4_4:73442376-73442395 None:intergenic 30.0%
!! CTCTTTATGAAAAAGTACTG+AGG - chr4_4:73442429-73442448 Msa0701990:intron 30.0%
GTAGATTGAATTGAACTCAA+GGG + chr4_4:73442531-73442550 None:intergenic 30.0%
AGTAGATTGAATTGAACTCA+AGG + chr4_4:73442532-73442551 None:intergenic 30.0%
CAATTCAATCTACTTTCTCT+TGG - chr4_4:73442538-73442557 Msa0701990:intron 30.0%
TGGAACATAAATTGTTATCG+GGG - chr4_4:73442558-73442577 Msa0701990:intron 30.0%
TACTTCAACTCAAACTTGTA+TGG + chr4_4:73442740-73442759 None:intergenic 30.0%
AGACATACAGACAATATTAG+AGG + chr4_4:73443182-73443201 None:intergenic 30.0%
ATAAATAGAATACAAGGCAG+TGG + chr4_4:73443225-73443244 None:intergenic 30.0%
! TGGACAATTGACATGTTTTA+AGG - chr4_4:73441510-73441529 Msa0701990:three_prime_UTR 30.0%
!! GGACAATTGACATGTTTTAA+GGG - chr4_4:73441511-73441530 Msa0701990:three_prime_UTR 30.0%
! TTCTTGTTGTCACAATCAAA+GGG + chr4_4:73441545-73441564 None:intergenic 30.0%
AACATCATCAACAACAAAGA+AGG - chr4_4:73441592-73441611 Msa0701990:three_prime_UTR 30.0%
AACAAAGAAGGAGGTTTATT+CGG - chr4_4:73441604-73441623 Msa0701990:three_prime_UTR 30.0%
!!! AAAACGTATATTTTGATGGG+TGG - chr4_4:73441996-73442015 Msa0701990:intron 30.0%
!!! AAACGTATATTTTGATGGGT+GGG - chr4_4:73441997-73442016 Msa0701990:intron 30.0%
TCATTCAGGTATATTGTGAT+CGG + chr4_4:73442067-73442086 None:intergenic 30.0%
TAATCAAGTAGCAAAGAGTT+TGG - chr4_4:73442118-73442137 Msa0701990:intron 30.0%
!! TTCATATCCTTGGATTAGTT+GGG - chr4_4:73442208-73442227 Msa0701990:intron 30.0%
! AATCTGCAACTTGTTCTTTA+AGG + chr4_4:73442284-73442303 None:intergenic 30.0%
TCATTGCGATTGAATTCATA+TGG - chr4_4:73442346-73442365 Msa0701990:intron 30.0%
!!! GTTTTTTTGATTGATTCACC+AGG + chr4_4:73442376-73442395 None:intergenic 30.0%
!! CTCTTTATGAAAAAGTACTG+AGG - chr4_4:73442429-73442448 Msa0701990:intron 30.0%
GTAGATTGAATTGAACTCAA+GGG + chr4_4:73442531-73442550 None:intergenic 30.0%
AGTAGATTGAATTGAACTCA+AGG + chr4_4:73442532-73442551 None:intergenic 30.0%
CAATTCAATCTACTTTCTCT+TGG - chr4_4:73442538-73442557 Msa0701990:intron 30.0%
TGGAACATAAATTGTTATCG+GGG - chr4_4:73442558-73442577 Msa0701990:intron 30.0%
TACTTCAACTCAAACTTGTA+TGG + chr4_4:73442740-73442759 None:intergenic 30.0%
AGACATACAGACAATATTAG+AGG + chr4_4:73443182-73443201 None:intergenic 30.0%
ATAAATAGAATACAAGGCAG+TGG + chr4_4:73443225-73443244 None:intergenic 30.0%
ATATTCCCATATTCCCTCTT+CGG + chr4_4:73441473-73441492 None:intergenic 35.0%
! CTTCTTGTTGTCACAATCAA+AGG + chr4_4:73441546-73441565 None:intergenic 35.0%
CTTTGATTGTGACAACAAGA+AGG - chr4_4:73441544-73441563 Msa0701990:three_prime_UTR 35.0%
!! TTGTTGATGATGTTGCCATT+CGG + chr4_4:73441586-73441605 None:intergenic 35.0%
ATCATCAACAACAAAGAAGG+AGG - chr4_4:73441595-73441614 Msa0701990:three_prime_UTR 35.0%
TAATCCAAGGATATGAAGGA+GGG + chr4_4:73442205-73442224 None:intergenic 35.0%
CAACTAATCCAAGGATATGA+AGG + chr4_4:73442209-73442228 None:intergenic 35.0%
!! CTTCATATCCTTGGATTAGT+TGG - chr4_4:73442207-73442226 Msa0701990:intron 35.0%
!!! GTGTGTGTGTGTATTTTGAT+AGG - chr4_4:73442837-73442856 Msa0701990:intron 35.0%
ACAATGTACACATTGGTACT+TGG + chr4_4:73442866-73442885 None:intergenic 35.0%
! TGTGATGGTATAAGTGTTGT+TGG + chr4_4:73443111-73443130 None:intergenic 35.0%
TTTCCTCATCATGTCATCTA+TGG + chr4_4:73443341-73443360 None:intergenic 35.0%
ATATTCCCATATTCCCTCTT+CGG + chr4_4:73441473-73441492 None:intergenic 35.0%
! CTTCTTGTTGTCACAATCAA+AGG + chr4_4:73441546-73441565 None:intergenic 35.0%
CTTTGATTGTGACAACAAGA+AGG - chr4_4:73441544-73441563 Msa0701990:three_prime_UTR 35.0%
!! TTGTTGATGATGTTGCCATT+CGG + chr4_4:73441586-73441605 None:intergenic 35.0%
ATCATCAACAACAAAGAAGG+AGG - chr4_4:73441595-73441614 Msa0701990:three_prime_UTR 35.0%
TAATCCAAGGATATGAAGGA+GGG + chr4_4:73442205-73442224 None:intergenic 35.0%
CAACTAATCCAAGGATATGA+AGG + chr4_4:73442209-73442228 None:intergenic 35.0%
!! CTTCATATCCTTGGATTAGT+TGG - chr4_4:73442207-73442226 Msa0701990:intron 35.0%
!!! GTGTGTGTGTGTATTTTGAT+AGG - chr4_4:73442837-73442856 Msa0701990:intron 35.0%
ACAATGTACACATTGGTACT+TGG + chr4_4:73442866-73442885 None:intergenic 35.0%
! TGTGATGGTATAAGTGTTGT+TGG + chr4_4:73443111-73443130 None:intergenic 35.0%
TTTCCTCATCATGTCATCTA+TGG + chr4_4:73443341-73443360 None:intergenic 35.0%
AGAGGGAATATGGGAATATG+CGG - chr4_4:73441474-73441493 Msa0701990:three_prime_UTR 40.0%
ATTGTGACAACAAGAAGGAG+AGG - chr4_4:73441549-73441568 Msa0701990:three_prime_UTR 40.0%
TAACACCTTCAGAAGCTGAT+CGG + chr4_4:73441778-73441797 None:intergenic 40.0%
! ACACTGATAAAAGAAGGTGC+CGG + chr4_4:73441834-73441853 None:intergenic 40.0%
! ATTGGTTTCCAGAATCTGAG+GGG + chr4_4:73441879-73441898 None:intergenic 40.0%
!! CATTGGTTTCCAGAATCTGA+GGG + chr4_4:73441880-73441899 None:intergenic 40.0%
TAGACGAAAGAGTCTCATTC+AGG + chr4_4:73442081-73442100 None:intergenic 40.0%
AATTCAAGACTTTGCCCGAA+GGG + chr4_4:73442146-73442165 None:intergenic 40.0%
TAATTCAAGACTTTGCCCGA+AGG + chr4_4:73442147-73442166 None:intergenic 40.0%
! TTTTCCCTCCTTCATATCCT+TGG - chr4_4:73442198-73442217 Msa0701990:intron 40.0%
CTAATCCAAGGATATGAAGG+AGG + chr4_4:73442206-73442225 None:intergenic 40.0%
!! TATCCTTGGATTAGTTGGGT+TGG - chr4_4:73442212-73442231 Msa0701990:intron 40.0%
!! ATCCTTGGATTAGTTGGGTT+GGG - chr4_4:73442213-73442232 Msa0701990:intron 40.0%
TTGCAGATTCAAGCGCAATA+TGG - chr4_4:73442295-73442314 Msa0701990:intron 40.0%
TTGAATTCATATGGCAACCC+TGG - chr4_4:73442355-73442374 Msa0701990:intron 40.0%
GGTAAAACTTCGTACTCCAA+TGG - chr4_4:73442450-73442469 Msa0701990:intron 40.0%
GGTGTTAGCTAATCCAATGA+AGG - chr4_4:73442579-73442598 Msa0701990:intron 40.0%
AGAGGTGACTACATCACTTA+TGG + chr4_4:73442626-73442645 None:intergenic 40.0%
CACTCAAATCTCCATACAGA+AGG + chr4_4:73442920-73442939 None:intergenic 40.0%
GAGTGATGAAGAGAAGCAAA+AGG - chr4_4:73442935-73442954 Msa0701990:CDS 40.0%
GCTTACTTATCTGAAAGCTC+AGG + chr4_4:73443001-73443020 None:intergenic 40.0%
CAACAGTTGTGAAGTTGTGA+TGG + chr4_4:73443126-73443145 None:intergenic 40.0%
AGAGGGAATATGGGAATATG+CGG - chr4_4:73441474-73441493 Msa0701990:three_prime_UTR 40.0%
ATTGTGACAACAAGAAGGAG+AGG - chr4_4:73441549-73441568 Msa0701990:three_prime_UTR 40.0%
TAACACCTTCAGAAGCTGAT+CGG + chr4_4:73441778-73441797 None:intergenic 40.0%
! ACACTGATAAAAGAAGGTGC+CGG + chr4_4:73441834-73441853 None:intergenic 40.0%
! ATTGGTTTCCAGAATCTGAG+GGG + chr4_4:73441879-73441898 None:intergenic 40.0%
!! CATTGGTTTCCAGAATCTGA+GGG + chr4_4:73441880-73441899 None:intergenic 40.0%
TAGACGAAAGAGTCTCATTC+AGG + chr4_4:73442081-73442100 None:intergenic 40.0%
AATTCAAGACTTTGCCCGAA+GGG + chr4_4:73442146-73442165 None:intergenic 40.0%
TAATTCAAGACTTTGCCCGA+AGG + chr4_4:73442147-73442166 None:intergenic 40.0%
! TTTTCCCTCCTTCATATCCT+TGG - chr4_4:73442198-73442217 Msa0701990:intron 40.0%
CTAATCCAAGGATATGAAGG+AGG + chr4_4:73442206-73442225 None:intergenic 40.0%
!! TATCCTTGGATTAGTTGGGT+TGG - chr4_4:73442212-73442231 Msa0701990:intron 40.0%
!! ATCCTTGGATTAGTTGGGTT+GGG - chr4_4:73442213-73442232 Msa0701990:intron 40.0%
TTGCAGATTCAAGCGCAATA+TGG - chr4_4:73442295-73442314 Msa0701990:intron 40.0%
TTGAATTCATATGGCAACCC+TGG - chr4_4:73442355-73442374 Msa0701990:intron 40.0%
GGTAAAACTTCGTACTCCAA+TGG - chr4_4:73442450-73442469 Msa0701990:intron 40.0%
GGTGTTAGCTAATCCAATGA+AGG - chr4_4:73442579-73442598 Msa0701990:intron 40.0%
AGAGGTGACTACATCACTTA+TGG + chr4_4:73442626-73442645 None:intergenic 40.0%
CACTCAAATCTCCATACAGA+AGG + chr4_4:73442920-73442939 None:intergenic 40.0%
GAGTGATGAAGAGAAGCAAA+AGG - chr4_4:73442935-73442954 Msa0701990:CDS 40.0%
GCTTACTTATCTGAAAGCTC+AGG + chr4_4:73443001-73443020 None:intergenic 40.0%
CAACAGTTGTGAAGTTGTGA+TGG + chr4_4:73443126-73443145 None:intergenic 40.0%
TGAAGACCGAAGAGGGAATA+TGG - chr4_4:73441464-73441483 Msa0701990:three_prime_UTR 45.0%
GAAGACCGAAGAGGGAATAT+GGG - chr4_4:73441465-73441484 Msa0701990:three_prime_UTR 45.0%
GAGGGAATATGGGAATATGC+GGG - chr4_4:73441475-73441494 Msa0701990:three_prime_UTR 45.0%
AGGGAATATGGGAATATGCG+GGG - chr4_4:73441476-73441495 Msa0701990:three_prime_UTR 45.0%
CAACAAGAAGGAGAGGTGAT+TGG - chr4_4:73441556-73441575 Msa0701990:three_prime_UTR 45.0%
! AGAAGGAGGTTTATTCGGTG+TGG - chr4_4:73441609-73441628 Msa0701990:three_prime_UTR 45.0%
!! GAAGGAGGTTTATTCGGTGT+GGG - chr4_4:73441610-73441629 Msa0701990:three_prime_UTR 45.0%
TAACTCTATTGCTCCCACGA+CGG + chr4_4:73441729-73441748 None:intergenic 45.0%
TTTGTTGAGTGCATCGTCTG+GGG + chr4_4:73441753-73441772 None:intergenic 45.0%
! GTTTGTTGAGTGCATCGTCT+GGG + chr4_4:73441754-73441773 None:intergenic 45.0%
!! AGTTTGTTGAGTGCATCGTC+TGG + chr4_4:73441755-73441774 None:intergenic 45.0%
AAACTCCGATCAGCTTCTGA+AGG - chr4_4:73441770-73441789 Msa0701990:CDS 45.0%
!! GCATTGGTTTCCAGAATCTG+AGG + chr4_4:73441881-73441900 None:intergenic 45.0%
!!! CGTATATTTTGATGGGTGGG+TGG - chr4_4:73442000-73442019 Msa0701990:intron 45.0%
! GCAAAGAGTTTGGTTCCCTT+CGG - chr4_4:73442128-73442147 Msa0701990:intron 45.0%
! CAAAGAGTTTGGTTCCCTTC+GGG - chr4_4:73442129-73442148 Msa0701990:intron 45.0%
TGAAGGAGGGAAAACTCTCT+CGG + chr4_4:73442192-73442211 None:intergenic 45.0%
!! TCCTTGGATTAGTTGGGTTG+GGG - chr4_4:73442214-73442233 Msa0701990:intron 45.0%
AAGTGACAAGTGTTCGCCAT+TGG + chr4_4:73442469-73442488 None:intergenic 45.0%
! CGAACACTTGTCACTTCACT+TGG - chr4_4:73442473-73442492 Msa0701990:intron 45.0%
ATACACACACACACAGACAC+TGG + chr4_4:73442831-73442850 None:intergenic 45.0%
ATTCTTGACGACAGTCTCAG+TGG - chr4_4:73442975-73442994 Msa0701990:CDS 45.0%
! GTGTTGTTGGCAAGCTAGTT+TGG + chr4_4:73443098-73443117 None:intergenic 45.0%
GTGCCATAGATGACATGATG+AGG - chr4_4:73443335-73443354 Msa0701990:five_prime_UTR 45.0%
TGAAGACCGAAGAGGGAATA+TGG - chr4_4:73441464-73441483 Msa0701990:three_prime_UTR 45.0%
GAAGACCGAAGAGGGAATAT+GGG - chr4_4:73441465-73441484 Msa0701990:three_prime_UTR 45.0%
GAGGGAATATGGGAATATGC+GGG - chr4_4:73441475-73441494 Msa0701990:three_prime_UTR 45.0%
AGGGAATATGGGAATATGCG+GGG - chr4_4:73441476-73441495 Msa0701990:three_prime_UTR 45.0%
CAACAAGAAGGAGAGGTGAT+TGG - chr4_4:73441556-73441575 Msa0701990:three_prime_UTR 45.0%
! AGAAGGAGGTTTATTCGGTG+TGG - chr4_4:73441609-73441628 Msa0701990:three_prime_UTR 45.0%
!! GAAGGAGGTTTATTCGGTGT+GGG - chr4_4:73441610-73441629 Msa0701990:three_prime_UTR 45.0%
TAACTCTATTGCTCCCACGA+CGG + chr4_4:73441729-73441748 None:intergenic 45.0%
TTTGTTGAGTGCATCGTCTG+GGG + chr4_4:73441753-73441772 None:intergenic 45.0%
! GTTTGTTGAGTGCATCGTCT+GGG + chr4_4:73441754-73441773 None:intergenic 45.0%
!! AGTTTGTTGAGTGCATCGTC+TGG + chr4_4:73441755-73441774 None:intergenic 45.0%
AAACTCCGATCAGCTTCTGA+AGG - chr4_4:73441770-73441789 Msa0701990:CDS 45.0%
!! GCATTGGTTTCCAGAATCTG+AGG + chr4_4:73441881-73441900 None:intergenic 45.0%
!!! CGTATATTTTGATGGGTGGG+TGG - chr4_4:73442000-73442019 Msa0701990:intron 45.0%
! GCAAAGAGTTTGGTTCCCTT+CGG - chr4_4:73442128-73442147 Msa0701990:intron 45.0%
! CAAAGAGTTTGGTTCCCTTC+GGG - chr4_4:73442129-73442148 Msa0701990:intron 45.0%
TGAAGGAGGGAAAACTCTCT+CGG + chr4_4:73442192-73442211 None:intergenic 45.0%
!! TCCTTGGATTAGTTGGGTTG+GGG - chr4_4:73442214-73442233 Msa0701990:intron 45.0%
AAGTGACAAGTGTTCGCCAT+TGG + chr4_4:73442469-73442488 None:intergenic 45.0%
! CGAACACTTGTCACTTCACT+TGG - chr4_4:73442473-73442492 Msa0701990:intron 45.0%
ATACACACACACACAGACAC+TGG + chr4_4:73442831-73442850 None:intergenic 45.0%
ATTCTTGACGACAGTCTCAG+TGG - chr4_4:73442975-73442994 Msa0701990:CDS 45.0%
! GTGTTGTTGGCAAGCTAGTT+TGG + chr4_4:73443098-73443117 None:intergenic 45.0%
GTGCCATAGATGACATGATG+AGG - chr4_4:73443335-73443354 Msa0701990:five_prime_UTR 45.0%
GAGGTGATTGGTACTCCGAA+TGG - chr4_4:73441568-73441587 Msa0701990:three_prime_UTR 50.0%
!! CTGATAAAAGAAGGTGCCGG+CGG + chr4_4:73441831-73441850 None:intergenic 50.0%
GTTTCCAGAATCTGAGGGGT+GGG + chr4_4:73441875-73441894 None:intergenic 50.0%
! AGTCACCTCTGAAGACTCTG+CGG - chr4_4:73442636-73442655 Msa0701990:intron 50.0%
GAGGTGATTGGTACTCCGAA+TGG - chr4_4:73441568-73441587 Msa0701990:three_prime_UTR 50.0%
!! CTGATAAAAGAAGGTGCCGG+CGG + chr4_4:73441831-73441850 None:intergenic 50.0%
GTTTCCAGAATCTGAGGGGT+GGG + chr4_4:73441875-73441894 None:intergenic 50.0%
! AGTCACCTCTGAAGACTCTG+CGG - chr4_4:73442636-73442655 Msa0701990:intron 50.0%
TGGGTGAGTGAAGACCGAAG+AGG - chr4_4:73441456-73441475 Msa0701990:three_prime_UTR 55.0%
GGGTGAGTGAAGACCGAAGA+GGG - chr4_4:73441457-73441476 Msa0701990:three_prime_UTR 55.0%
ATGGGAATATGCGGGGCATG+TGG - chr4_4:73441483-73441502 Msa0701990:three_prime_UTR 55.0%
TCCACCGAATTCGGATCGGA+GGG + chr4_4:73441684-73441703 None:intergenic 55.0%
GAACTGGGGTCCACCGAATT+CGG + chr4_4:73441693-73441712 None:intergenic 55.0%
GTCATGTGGGGTTCGAACTG+GGG + chr4_4:73441707-73441726 None:intergenic 55.0%
GGTCATGTGGGGTTCGAACT+GGG + chr4_4:73441708-73441727 None:intergenic 55.0%
CAACACGGTCAACGGTGACT+TGG + chr4_4:73441808-73441827 None:intergenic 55.0%
TCTGAGGGGTGGGATGAAGA+AGG + chr4_4:73441865-73441884 None:intergenic 55.0%
TCATCCCACCCCTCAGATTC+TGG - chr4_4:73441868-73441887 Msa0701990:CDS 55.0%
GGTTTCCAGAATCTGAGGGG+TGG + chr4_4:73441876-73441895 None:intergenic 55.0%
ATGGGTGCAGCAGTGTGCAT+TGG + chr4_4:73441897-73441916 None:intergenic 55.0%
GCACACTGCTGCACCCATTT+CGG - chr4_4:73441899-73441918 Msa0701990:CDS 55.0%
GCCCCAACCCAACTAATCCA+AGG + chr4_4:73442218-73442237 None:intergenic 55.0%
CAAGTCCGCAGAGTCTTCAG+AGG + chr4_4:73442644-73442663 None:intergenic 55.0%
! CTCTGAAGACTCTGCGGACT+TGG - chr4_4:73442642-73442661 Msa0701990:intron 55.0%
CCATACAGAAGGCTCACGTG+TGG + chr4_4:73442909-73442928 None:intergenic 55.0%
CCACACGTGAGCCTTCTGTA+TGG - chr4_4:73442906-73442925 Msa0701990:CDS 55.0%
TGGGTGAGTGAAGACCGAAG+AGG - chr4_4:73441456-73441475 Msa0701990:three_prime_UTR 55.0%
GGGTGAGTGAAGACCGAAGA+GGG - chr4_4:73441457-73441476 Msa0701990:three_prime_UTR 55.0%
ATGGGAATATGCGGGGCATG+TGG - chr4_4:73441483-73441502 Msa0701990:three_prime_UTR 55.0%
TCCACCGAATTCGGATCGGA+GGG + chr4_4:73441684-73441703 None:intergenic 55.0%
GAACTGGGGTCCACCGAATT+CGG + chr4_4:73441693-73441712 None:intergenic 55.0%
GTCATGTGGGGTTCGAACTG+GGG + chr4_4:73441707-73441726 None:intergenic 55.0%
GGTCATGTGGGGTTCGAACT+GGG + chr4_4:73441708-73441727 None:intergenic 55.0%
CAACACGGTCAACGGTGACT+TGG + chr4_4:73441808-73441827 None:intergenic 55.0%
TCTGAGGGGTGGGATGAAGA+AGG + chr4_4:73441865-73441884 None:intergenic 55.0%
TCATCCCACCCCTCAGATTC+TGG - chr4_4:73441868-73441887 Msa0701990:CDS 55.0%
GGTTTCCAGAATCTGAGGGG+TGG + chr4_4:73441876-73441895 None:intergenic 55.0%
ATGGGTGCAGCAGTGTGCAT+TGG + chr4_4:73441897-73441916 None:intergenic 55.0%
GCACACTGCTGCACCCATTT+CGG - chr4_4:73441899-73441918 Msa0701990:CDS 55.0%
GCCCCAACCCAACTAATCCA+AGG + chr4_4:73442218-73442237 None:intergenic 55.0%
CAAGTCCGCAGAGTCTTCAG+AGG + chr4_4:73442644-73442663 None:intergenic 55.0%
! CTCTGAAGACTCTGCGGACT+TGG - chr4_4:73442642-73442661 Msa0701990:intron 55.0%
CCATACAGAAGGCTCACGTG+TGG + chr4_4:73442909-73442928 None:intergenic 55.0%
CCACACGTGAGCCTTCTGTA+TGG - chr4_4:73442906-73442925 Msa0701990:CDS 55.0%
TATGCGGGGCATGTGGCACA+TGG - chr4_4:73441490-73441509 Msa0701990:three_prime_UTR 60.0%
GGTCGCATACGTCTTCCGGT+GGG + chr4_4:73441640-73441659 None:intergenic 60.0%
AGGCGGTCGCATACGTCTTC+CGG + chr4_4:73441644-73441663 None:intergenic 60.0%
! CGGTCATGAGCTTGGTGAGG+CGG + chr4_4:73441661-73441680 None:intergenic 60.0%
ACCGCCCTCCGATCCGAATT+CGG - chr4_4:73441677-73441696 Msa0701990:three_prime_UTR 60.0%
ACCGAATTCGGATCGGAGGG+CGG + chr4_4:73441681-73441700 None:intergenic 60.0%
GTCCACCGAATTCGGATCGG+AGG + chr4_4:73441685-73441704 None:intergenic 60.0%
GGGGTCCACCGAATTCGGAT+CGG + chr4_4:73441688-73441707 None:intergenic 60.0%
CGGTCATGTGGGGTTCGAAC+TGG + chr4_4:73441709-73441728 None:intergenic 60.0%
GAACCCCACATGACCGTCGT+GGG - chr4_4:73441713-73441732 Msa0701990:exon 60.0%
TGCTCCCACGACGGTCATGT+GGG + chr4_4:73441720-73441739 None:intergenic 60.0%
TTGCTCCCACGACGGTCATG+TGG + chr4_4:73441721-73441740 None:intergenic 60.0%
TATGCGGGGCATGTGGCACA+TGG - chr4_4:73441490-73441509 Msa0701990:three_prime_UTR 60.0%
GGTCGCATACGTCTTCCGGT+GGG + chr4_4:73441640-73441659 None:intergenic 60.0%
AGGCGGTCGCATACGTCTTC+CGG + chr4_4:73441644-73441663 None:intergenic 60.0%
! CGGTCATGAGCTTGGTGAGG+CGG + chr4_4:73441661-73441680 None:intergenic 60.0%
ACCGCCCTCCGATCCGAATT+CGG - chr4_4:73441677-73441696 Msa0701990:three_prime_UTR 60.0%
ACCGAATTCGGATCGGAGGG+CGG + chr4_4:73441681-73441700 None:intergenic 60.0%
GTCCACCGAATTCGGATCGG+AGG + chr4_4:73441685-73441704 None:intergenic 60.0%
GGGGTCCACCGAATTCGGAT+CGG + chr4_4:73441688-73441707 None:intergenic 60.0%
CGGTCATGTGGGGTTCGAAC+TGG + chr4_4:73441709-73441728 None:intergenic 60.0%
GAACCCCACATGACCGTCGT+GGG - chr4_4:73441713-73441732 Msa0701990:exon 60.0%
TGCTCCCACGACGGTCATGT+GGG + chr4_4:73441720-73441739 None:intergenic 60.0%
TTGCTCCCACGACGGTCATG+TGG + chr4_4:73441721-73441740 None:intergenic 60.0%
! TTCGGTGTGGGCGATCCCAC+CGG - chr4_4:73441622-73441641 Msa0701990:three_prime_UTR 65.0%
CGGTCGCATACGTCTTCCGG+TGG + chr4_4:73441641-73441660 None:intergenic 65.0%
! GGGCGGTCATGAGCTTGGTG+AGG + chr4_4:73441664-73441683 None:intergenic 65.0%
TCGGAGGGCGGTCATGAGCT+TGG + chr4_4:73441669-73441688 None:intergenic 65.0%
GCCCTCCGATCCGAATTCGG+TGG - chr4_4:73441680-73441699 Msa0701990:three_prime_UTR 65.0%
CGAACCCCACATGACCGTCG+TGG - chr4_4:73441712-73441731 Msa0701990:exon 65.0%
GCTCCCACGACGGTCATGTG+GGG + chr4_4:73441719-73441738 None:intergenic 65.0%
ACCGTTGACCGTGTTGCCGC+CGG - chr4_4:73441812-73441831 Msa0701990:CDS 65.0%
!! AGAAGGTGCCGGCGGCAACA+CGG + chr4_4:73441823-73441842 None:intergenic 65.0%
! TTCGGTGTGGGCGATCCCAC+CGG - chr4_4:73441622-73441641 Msa0701990:three_prime_UTR 65.0%
CGGTCGCATACGTCTTCCGG+TGG + chr4_4:73441641-73441660 None:intergenic 65.0%
! GGGCGGTCATGAGCTTGGTG+AGG + chr4_4:73441664-73441683 None:intergenic 65.0%
TCGGAGGGCGGTCATGAGCT+TGG + chr4_4:73441669-73441688 None:intergenic 65.0%
GCCCTCCGATCCGAATTCGG+TGG - chr4_4:73441680-73441699 Msa0701990:three_prime_UTR 65.0%
CGAACCCCACATGACCGTCG+TGG - chr4_4:73441712-73441731 Msa0701990:exon 65.0%
GCTCCCACGACGGTCATGTG+GGG + chr4_4:73441719-73441738 None:intergenic 65.0%
ACCGTTGACCGTGTTGCCGC+CGG - chr4_4:73441812-73441831 Msa0701990:CDS 65.0%
!! AGAAGGTGCCGGCGGCAACA+CGG + chr4_4:73441823-73441842 None:intergenic 65.0%
GCCGGCGGCAACACGGTCAA+CGG + chr4_4:73441816-73441835 None:intergenic 70.0%
GCCGGCGGCAACACGGTCAA+CGG + chr4_4:73441816-73441835 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 73441450 73443375 73441450 ID=Msa0701990;Name=Msa0701990
chr4_4 mRNA 73441450 73443375 73441450 ID=Msa0701990-mRNA-1;Parent=Msa0701990;Name=Msa0701990-mRNA-1;_AED=0.35;_eAED=0.35;_QI=137|1|1|1|1|1|2|272|185
chr4_4 exon 73441450 73441927 73441450 ID=Msa0701990-mRNA-1:exon:22646;Parent=Msa0701990-mRNA-1
chr4_4 exon 73442887 73443375 73442887 ID=Msa0701990-mRNA-1:exon:22645;Parent=Msa0701990-mRNA-1
chr4_4 five_prime_UTR 73443239 73443375 73443239 ID=Msa0701990-mRNA-1:five_prime_utr;Parent=Msa0701990-mRNA-1
chr4_4 CDS 73442887 73443238 73442887 ID=Msa0701990-mRNA-1:cds;Parent=Msa0701990-mRNA-1
chr4_4 CDS 73441722 73441927 73441722 ID=Msa0701990-mRNA-1:cds;Parent=Msa0701990-mRNA-1
chr4_4 three_prime_UTR 73441450 73441721 73441450 ID=Msa0701990-mRNA-1:three_prime_utr;Parent=Msa0701990-mRNA-1
Gene Sequence

>Msa0701990

ATGGCAACATCATCAACAACAAAGAAGGAGGTTTATTCGGTGTGGGCGATCCCACCGGAAGACGTATGCGACCGCCTCACCAAGCTCATGACCGCCCTCCGATCCGAATTCGGTGGACCCCAGTTCGAACCCCACATGACCGTCGTGGGAGCAATAGAGTTAACCCCAGACGATGCACTCAACAAACTCCGATCAGCTTCTGAAGGTGTTAAGTCTTTCCAAGTCACCGTTGACCGTGTTGCCGCCGGCACCTTCTTTTATCAGTGTGTTTATCTCCTTCTTCATCCCACCCCTCAGATTCTGGAAACCAATGCACACTGCTGCACCCATTTCGGTTATAACAATTCAACTCCTTACATGCCACACGTGAGCCTTCTGTATGGAGATTTGAGTGATGAAGAGAAGCAAAAGGCTCAAGAAAGAGCTAATATTCTTGACGACAGTCTCAGTGGCCTGAGCTTTCAGATAAGTAAGCTTGCATTATACAAAACAGACACTGAAGACAAATCTCTTAAATCTTGGGAGAAAATTGCTGAATGCACTCTTACTCCAAACTAG
Protein sequence

>Msa0701990

MATSSTTKKEVYSVWAIPPEDVCDRLTKLMTALRSEFGGPQFEPHMTVVGAIELTPDDALNKLRSASEGVKSFQVTVDRVAAGTFFYQCVYLLLHPTPQILETNAHCCTHFGYNNSTPYMPHVSLLYGDLSDEEKQKAQERANILDDSLSGLSFQISKLALYKTDTEDKSLKSWEKIAECTLTPN*