AlfalfaGEDB | Msa0704630

M. sativa cultivar zhongmu-4 / Msa0704630

KEGG: K16282
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009628 GO:0009651 GO:0009787 GO:0009789 GO:0009966 GO:0009967 GO:0009987 GO:0010498 GO:0010646 GO:0010647 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0023051 GO:0023056 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080134 GO:0140096 GO:1901419 GO:1901421 GO:1901564 GO:1901565 GO:1901575 GO:1905957 GO:1905959
PFAM: PF13639.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0704630	RHN42975.1	94.268	157	9	0	1	157	1	157	4.58e-104	304

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0704630	sp\|Q9ZT50\|RHA2A_ARATH	54.386	57	24	1	67	123	85	139	5.77e-14	68.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0704630	A0A396GVX9	94.268	157	9	0	1	157	1	157	2.19e-104	304

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0704630	Msa1358450	0.949017	2.971853e-107	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0704630	MtrunA17_Chr8g0382811	94.268	157	9	0	1	157	1	157	4.21e-108	304
Msa0704630	MtrunA17_Chr3g0124531	50.327	153	72	3	1	151	1	151	3.08e-44	143
Msa0704630	MtrunA17_Chr0c01g0489421	46.032	63	32	1	62	124	51	111	1.94e-14	66.2
Msa0704630	MtrunA17_Chr8g0387291	34.559	136	71	5	5	124	8	141	3.68e-14	66.2
Msa0704630	MtrunA17_Chr2g0327791	41.304	92	44	5	54	138	117	205	5.36e-14	66.6
Msa0704630	MtrunA17_Chr4g0030831	44.595	74	33	3	54	122	94	164	1.40e-13	64.7
Msa0704630	MtrunA17_Chr5g0408871	42.647	68	36	3	57	123	494	559	7.03e-13	65.1
Msa0704630	MtrunA17_Chr5g0427771	42.500	80	39	2	33	112	29	101	7.19e-13	62.0
Msa0704630	MtrunA17_Chr5g0427781	39.759	83	43	2	33	115	29	104	2.00e-12	60.8
Msa0704630	MtrunA17_Chr4g0039541	34.615	104	64	1	16	115	2	105	2.52e-12	60.5
Msa0704630	MtrunA17_Chr5g0425981	34.286	105	58	2	22	117	2	104	9.04e-12	59.3
Msa0704630	MtrunA17_Chr0c01g0489441	35.227	88	44	1	30	117	49	123	9.44e-12	59.3
Msa0704630	MtrunA17_Chr4g0067591	50.000	58	25	2	64	120	100	154	1.53e-11	60.5
Msa0704630	MtrunA17_Chr4g0065171	50.000	48	22	2	68	115	484	529	1.87e-11	60.8
Msa0704630	MtrunA17_Chr3g0104311	42.623	61	33	1	64	124	54	112	2.61e-11	57.8
Msa0704630	MtrunA17_Chr1g0171061	45.455	55	28	1	66	120	55	107	4.99e-11	57.4
Msa0704630	MtrunA17_Chr7g0246571	48.276	58	27	1	62	119	306	360	6.83e-11	59.3
Msa0704630	MtrunA17_Chr1g0209001	43.333	60	32	1	60	119	87	144	7.67e-11	57.4
Msa0704630	MtrunA17_Chr8g0369691	31.304	115	61	5	4	115	391	490	7.76e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0704630	AT1G15100.1	54.386	57	24	1	67	123	85	139	5.87e-15	68.2
Msa0704630	AT5G45290.2	48.214	56	28	1	68	123	490	544	6.78e-14	68.6
Msa0704630	AT3G16720.1	56.364	55	20	2	64	117	115	166	1.76e-12	63.9
Msa0704630	AT5G45290.1	48.214	56	27	2	68	123	490	543	2.02e-12	64.3
Msa0704630	AT5G41400.1	46.552	58	29	2	68	124	105	161	1.63e-11	59.7
Msa0704630	AT4G00305.1	41.538	65	31	3	55	118	62	120	2.07e-11	58.2
Msa0704630	AT1G72310.1	45.902	61	29	2	64	123	123	180	2.89e-11	60.5
Msa0704630	AT2G20030.1	46.875	64	29	3	60	121	116	176	5.73e-11	60.1
Msa0704630	AT1G23980.1	50.847	59	25	2	60	117	136	191	7.59e-11	59.7

Find 28 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTGAGAGGTGGTAGTATT+TGG	0.218290	4_4:+77094073	Msa0704630:CDS
AGGTATTGGGTTGTGGTAAT+TGG	0.266040	4_4:-77093751	None:intergenic
GTGCTACTTGTCCACTTTGC+AGG	0.272706	4_4:+77093950	Msa0704630:CDS
CACATTTCATAAGGATTGTT+TGG	0.286072	4_4:+77093900	Msa0704630:CDS
CTTCAATGAAATTGAGGTAT+TGG	0.288998	4_4:-77093765	None:intergenic
TTGAGGAAGGGGACATTGTT+AGG	0.300056	4_4:+77093860	Msa0704630:CDS
TTCAATGAAATTGAGGTATT+GGG	0.316853	4_4:-77093764	None:intergenic
ATAAGGATTGTTTGGACAAA+TGG	0.327903	4_4:+77093908	Msa0704630:CDS
CGATCAGTATATATGTAAAT+AGG	0.332800	4_4:-77093687	None:intergenic
GTATTGTTTGCTTCAAAATC+AGG	0.367220	4_4:+77094005	Msa0704630:CDS
CAAAATCAGGTAGAATTTGA+TGG	0.399273	4_4:+77094018	Msa0704630:CDS
GGCTTCTTCAACCTTCTAGT+GGG	0.406833	4_4:-77093796	None:intergenic
CAATCTATGTGCTCTGCTGC+AGG	0.424192	4_4:-77093817	None:intergenic
CTGTGAACACACATTTCATA+AGG	0.434557	4_4:+77093891	Msa0704630:CDS
TCTTCTCTTCAATGAAATTG+AGG	0.435803	4_4:-77093771	None:intergenic
TGTTTATCTGAGTTTGAGGA+AGG	0.453729	4_4:+77093847	Msa0704630:CDS
CAGCAGAGCACATAGATTGT+AGG	0.454258	4_4:+77093821	Msa0704630:CDS
GGTATGTTTATCTGAGTTTG+AGG	0.460224	4_4:+77093843	Msa0704630:CDS
TTTATCTGAGTTTGAGGAAG+GGG	0.469479	4_4:+77093849	Msa0704630:CDS
AAGAGAAGAACCCCACTAGA+AGG	0.481865	4_4:+77093785	Msa0704630:CDS
AAAATCAGGTAGAATTTGAT+GGG	0.490367	4_4:+77094019	Msa0704630:CDS
AGGCTTCTTCAACCTTCTAG+TGG	0.505384	4_4:-77093797	None:intergenic
GAAATTGAGGTATTGGGTTG+TGG	0.517680	4_4:-77093758	None:intergenic
AAAACTTTGTTCCTGCAAAG+TGG	0.534572	4_4:-77093961	None:intergenic
AGCAGAGCACATAGATTGTA+GGG	0.548771	4_4:+77093822	Msa0704630:CDS
GCTTCTTCAACCTTCTAGTG+GGG	0.573302	4_4:-77093795	None:intergenic
GTTTATCTGAGTTTGAGGAA+GGG	0.622505	4_4:+77093848	Msa0704630:CDS
ACTTTAGGTAACAAATGACA+AGG	0.685765	4_4:-77093717	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CGATCAGTATATATGTAAAT+AGG	-	chr4_4:77093690-77093709	None:intergenic	25.0%
!!!	TTGGATTTTTAGTTGACTTT+AGG	-	chr4_4:77093735-77093754	None:intergenic	25.0%
!!	TTCAATGAAATTGAGGTATT+GGG	-	chr4_4:77093767-77093786	None:intergenic	25.0%
!	AAAATCAGGTAGAATTTGAT+GGG	+	chr4_4:77094019-77094038	Msa0704630:CDS	25.0%
!!!	AGTTTGTTATTTTCATTGAG+AGG	+	chr4_4:77094060-77094079	Msa0704630:CDS	25.0%
	ACTTTAGGTAACAAATGACA+AGG	-	chr4_4:77093720-77093739	None:intergenic	30.0%
	CTTCAATGAAATTGAGGTAT+TGG	-	chr4_4:77093768-77093787	None:intergenic	30.0%
	TCTTCTCTTCAATGAAATTG+AGG	-	chr4_4:77093774-77093793	None:intergenic	30.0%
	CACATTTCATAAGGATTGTT+TGG	+	chr4_4:77093900-77093919	Msa0704630:CDS	30.0%
	ATAAGGATTGTTTGGACAAA+TGG	+	chr4_4:77093908-77093927	Msa0704630:CDS	30.0%
	GTATTGTTTGCTTCAAAATC+AGG	+	chr4_4:77094005-77094024	Msa0704630:CDS	30.0%
	CAAAATCAGGTAGAATTTGA+TGG	+	chr4_4:77094018-77094037	Msa0704630:CDS	30.0%
!!!	TTGTTATTTTCATTGAGAGG+TGG	+	chr4_4:77094063-77094082	Msa0704630:CDS	30.0%
	GGTATGTTTATCTGAGTTTG+AGG	+	chr4_4:77093843-77093862	Msa0704630:CDS	35.0%
	TGTTTATCTGAGTTTGAGGA+AGG	+	chr4_4:77093847-77093866	Msa0704630:CDS	35.0%
	GTTTATCTGAGTTTGAGGAA+GGG	+	chr4_4:77093848-77093867	Msa0704630:CDS	35.0%
	TTTATCTGAGTTTGAGGAAG+GGG	+	chr4_4:77093849-77093868	Msa0704630:CDS	35.0%
	CTGTGAACACACATTTCATA+AGG	+	chr4_4:77093891-77093910	Msa0704630:CDS	35.0%
	AAAACTTTGTTCCTGCAAAG+TGG	-	chr4_4:77093964-77093983	None:intergenic	35.0%
!!	TTGCAGGAACAAAGTTTTGT+CGG	+	chr4_4:77093966-77093985	Msa0704630:CDS	35.0%
!!	AGGTATTGGGTTGTGGTAAT+TGG	-	chr4_4:77093754-77093773	None:intergenic	40.0%
!!	GAAATTGAGGTATTGGGTTG+TGG	-	chr4_4:77093761-77093780	None:intergenic	40.0%
	AGCAGAGCACATAGATTGTA+GGG	+	chr4_4:77093822-77093841	Msa0704630:CDS	40.0%
	CATTGAGAGGTGGTAGTATT+TGG	+	chr4_4:77094073-77094092	Msa0704630:CDS	40.0%
	AAGAGAAGAACCCCACTAGA+AGG	+	chr4_4:77093785-77093804	Msa0704630:CDS	45.0%
	GCTTCTTCAACCTTCTAGTG+GGG	-	chr4_4:77093798-77093817	None:intergenic	45.0%
	GGCTTCTTCAACCTTCTAGT+GGG	-	chr4_4:77093799-77093818	None:intergenic	45.0%
	AGGCTTCTTCAACCTTCTAG+TGG	-	chr4_4:77093800-77093819	None:intergenic	45.0%
	CAGCAGAGCACATAGATTGT+AGG	+	chr4_4:77093821-77093840	Msa0704630:CDS	45.0%
	TTGAGGAAGGGGACATTGTT+AGG	+	chr4_4:77093860-77093879	Msa0704630:CDS	45.0%
	CAATCTATGTGCTCTGCTGC+AGG	-	chr4_4:77093820-77093839	None:intergenic	50.0%
!!	GTGCTACTTGTCCACTTTGC+AGG	+	chr4_4:77093950-77093969	Msa0704630:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	77093637	77094110	77093637	ID=Msa0704630;Name=Msa0704630
chr4_4	mRNA	77093637	77094110	77093637	ID=Msa0704630-mRNA-1;Parent=Msa0704630;Name=Msa0704630-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|157
chr4_4	exon	77093637	77094110	77093637	ID=Msa0704630-mRNA-1:exon:24347;Parent=Msa0704630-mRNA-1
chr4_4	CDS	77093637	77094110	77093637	ID=Msa0704630-mRNA-1:cds;Parent=Msa0704630-mRNA-1

Gene Sequence

>Msa0704630

ATGACAATTCTCTCTGAGCTCTTATATCAGCTTTATGTCAAAACACTAGTCCTATTTACATATATACTGATCGAATTTATCCTTGTCATTTGTTACCTAAAGTCAACTAAAAATCCAATTACCACAACCCAATACCTCAATTTCATTGAAGAGAAGAACCCCACTAGAAGGTTGAAGAAGCCTGCAGCAGAGCACATAGATTGTAGGGTATGTTTATCTGAGTTTGAGGAAGGGGACATTGTTAGGAGCTTGAACTGTGAACACACATTTCATAAGGATTGTTTGGACAAATGGTTCTTGCAAGAACAATATTGTGCTACTTGTCCACTTTGCAGGAACAAAGTTTTGTCGGACGATGTTGCTTCAAAGTATTGTTTGCTTCAAAATCAGGTAGAATTTGATGGGATTGATGATGAGTTTATGAGTTTGTTATTTTCATTGAGAGGTGGTAGTATTTGGTATAGATATCTCTGA

Protein sequence

>Msa0704630

MTILSELLYQLYVKTLVLFTYILIEFILVICYLKSTKNPITTTQYLNFIEEKNPTRRLKKPAAEHIDCRVCLSEFEEGDIVRSLNCEHTFHKDCLDKWFLQEQYCATCPLCRNKVLSDDVASKYCLLQNQVEFDGIDDEFMSLLFSLRGGSIWYRYL*