AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0707730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0707730 MtrunA17_Chr8g0389961 98.438 128 2 0 7 134 464 591 1.23e-86 263
Msa0707730 MtrunA17_Chr3g0140501 80.469 128 25 0 7 134 451 578 4.20e-64 204
Msa0707730 MtrunA17_Chr2g0331051 77.344 128 29 0 7 134 514 641 2.92e-61 198
Msa0707730 MtrunA17_Chr8g0340671 74.219 128 33 0 7 134 489 616 9.99e-58 188
Msa0707730 MtrunA17_Chr7g0247001 40.152 132 66 3 11 130 464 594 1.18e-22 92.4
Msa0707730 MtrunA17_Chr8g0337031 41.667 132 64 3 11 130 490 620 6.08e-21 87.4
Msa0707730 MtrunA17_Chr8g0349861 38.168 131 70 3 11 130 467 597 1.07e-20 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0707730 AT5G25880.1 77.344 128 29 0 7 134 461 588 8.33e-68 214
Msa0707730 AT2G19900.2 78.125 128 28 0 7 134 355 482 3.50e-60 192
Msa0707730 AT2G19900.1 78.125 128 28 0 7 134 454 581 2.72e-59 192
Msa0707730 AT5G11670.1 77.344 128 29 0 7 134 461 588 1.14e-58 191
Msa0707730 AT1G79750.1 75.000 128 32 0 7 134 519 646 1.47e-57 189
Msa0707730 AT5G25880.2 78.000 100 22 0 7 106 461 560 5.57e-50 167
Msa0707730 AT2G13560.1 45.038 131 61 3 11 130 485 615 1.25e-24 98.2
Msa0707730 AT4G00570.1 41.481 135 66 3 8 130 467 600 3.46e-24 97.1

Find 51 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATTGACTCTGAAGCTTT+TGG 0.254889 4_4:-81144472 Msa0707730:three_prime_UTR
ATCTTCCCAGGCTTTGGTTT+GGG 0.270502 4_4:-81145009 Msa0707730:CDS
TCTATTAAGGATGTTGCTTT+TGG 0.307986 4_4:-81144448 Msa0707730:three_prime_UTR
TTATTCTTAACAAGCATAAA+AGG 0.314527 4_4:+81144415 None:intergenic
GCTTACATCTTCCCAGGCTT+TGG 0.317558 4_4:-81145015 Msa0707730:CDS
GAATGACATGAGATGAAATA+AGG 0.332301 4_4:+81144340 None:intergenic
GACCGAGGAGAACTACAAAA+AGG 0.333516 4_4:-81144788 Msa0707730:CDS
ACAGATAGACCGTTATGATA+TGG 0.341932 4_4:-81144205 Msa0707730:three_prime_UTR
CAGTTATTTGCTTGCAACTT+TGG 0.346581 4_4:-81144258 Msa0707730:three_prime_UTR
CATCTTCCCAGGCTTTGGTT+TGG 0.357949 4_4:-81145010 Msa0707730:CDS
AAAGGCAAAACTTATTATTC+TGG 0.358566 4_4:-81145133 Msa0707730:CDS
ATTTGCTTGCAACTTTGGTT+TGG 0.383748 4_4:-81144253 Msa0707730:three_prime_UTR
ATGAAATAAGGATATCATAA+TGG 0.396404 4_4:+81144352 None:intergenic
TGGGAAGATGTAAGCATTGT+TGG 0.397937 4_4:+81145023 None:intergenic
CAGATAGACCGTTATGATAT+GGG 0.416115 4_4:-81144204 Msa0707730:three_prime_UTR
CCCCTCTGTACCGCAACTAT+AGG 0.418903 4_4:-81144511 Msa0707730:CDS
TTGTATTCAACAGGATCAAA+TGG 0.426872 4_4:+81145154 None:intergenic
TCATAATGGAAAGAACATAT+TGG 0.434150 4_4:+81144366 None:intergenic
CTTTCTCAAACCGTTTCCTT+AGG 0.435632 4_4:+81144292 None:intergenic
TGGTTTGGGTATAGTGATGT+CGG 0.471357 4_4:-81144995 Msa0707730:CDS
TCCAAAAGCTTCAGAGTCAA+TGG 0.473079 4_4:+81144471 None:intergenic
GGTTTGGGTATAGTGATGTC+GGG 0.476580 4_4:-81144994 Msa0707730:CDS
AACTTTGGTTTGGTTTAACA+TGG 0.477925 4_4:-81144243 Msa0707730:three_prime_UTR
CACACCTAGTTCATATGCCT+TGG 0.478256 4_4:+81144699 None:intergenic
CAAAACTTATTATTCTGGCC+AGG 0.488294 4_4:-81145128 Msa0707730:intron
ACTATACCCAAACCAAAGCC+TGG 0.496340 4_4:+81145003 None:intergenic
AGCTGCAAATGTTGCTGCCA+AGG 0.499193 4_4:-81144716 Msa0707730:CDS
GCATACTTGACAAGATTCTC+AGG 0.501166 4_4:+81144551 None:intergenic
TTACAAGTTGCCTAAGGAAA+CGG 0.502495 4_4:-81144302 Msa0707730:three_prime_UTR
TATGGTTGCAGCGGAAGCCT+TGG 0.505776 4_4:-81144821 Msa0707730:intron
AGCAACATTTGCAGCTATGT+TGG 0.509394 4_4:+81144723 None:intergenic
ACCTATAGTTGCGGTACAGA+GGG 0.514198 4_4:+81144510 None:intergenic
CTCGGTCACTTGCATCGCCA+AGG 0.525665 4_4:+81144804 None:intergenic
AACAATGCTTACATCTTCCC+AGG 0.526563 4_4:-81145021 Msa0707730:CDS
CCCTTGTTCACCTATAGTTG+CGG 0.533999 4_4:+81144501 None:intergenic
CATAACGGTCTATCTGTACA+TGG 0.534989 4_4:+81144211 None:intergenic
TTTGATCCTGTTGAATACAA+AGG 0.555630 4_4:-81145151 Msa0707730:CDS
CTATCTGTACATGGTAAAAG+AGG 0.572958 4_4:+81144220 None:intergenic
CACCTATAGTTGCGGTACAG+AGG 0.578766 4_4:+81144509 None:intergenic
CTATACCCAAACCAAAGCCT+GGG 0.594858 4_4:+81145004 None:intergenic
TATCAATCCCCATATCATAA+CGG 0.595461 4_4:+81144196 None:intergenic
GAAGCATATCATCATGTACT+CGG 0.608942 4_4:+81144965 None:intergenic
GCTGCCAAGGCATATGAACT+AGG 0.617490 4_4:-81144703 Msa0707730:intron
ACCGCAACTATAGGTGAACA+AGG 0.619842 4_4:-81144502 Msa0707730:exon
TGTGTGTTACAAGTTGCCTA+AGG 0.621008 4_4:-81144308 Msa0707730:three_prime_UTR
TTGACAAGATTCTCAGGACG+AGG 0.639476 4_4:+81144557 None:intergenic
AGATAGACCGTTATGATATG+GGG 0.640565 4_4:-81144203 Msa0707730:three_prime_UTR
CTTGGCGATGCAAGTGACCG+AGG 0.665477 4_4:-81144803 Msa0707730:CDS
TTTCTGATATCAGAGAAAGG+TGG 0.681242 4_4:+81144754 None:intergenic
CCTATAGTTGCGGTACAGAG+GGG 0.701436 4_4:+81144511 None:intergenic
CCGCAACTATAGGTGAACAA+GGG 0.735107 4_4:-81144501 Msa0707730:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTCATATTAATAACTTAC+AGG + chr4_4:81144428-81144447 None:intergenic 20.0%
!! GAAAAAAAAAATACATGTCT+GGG + chr4_4:81144728-81144747 None:intergenic 20.0%
!! TTATTCTTAACAAGCATAAA+AGG + chr4_4:81144949-81144968 None:intergenic 20.0%
!! ATGAAATAAGGATATCATAA+TGG + chr4_4:81145012-81145031 None:intergenic 20.0%
!! TTCTCATATTAATAACTTAC+AGG + chr4_4:81144428-81144447 None:intergenic 20.0%
!! GAAAAAAAAAATACATGTCT+GGG + chr4_4:81144728-81144747 None:intergenic 20.0%
!! TTATTCTTAACAAGCATAAA+AGG + chr4_4:81144949-81144968 None:intergenic 20.0%
!! ATGAAATAAGGATATCATAA+TGG + chr4_4:81145012-81145031 None:intergenic 20.0%
! AAAGGCAAAACTTATTATTC+TGG - chr4_4:81144228-81144247 Msa0707730:three_prime_UTR 25.0%
! AAGGAAATAATCAGATACAA+TGG + chr4_4:81144307-81144326 None:intergenic 25.0%
!! ATTTTTCTGATATCAGAGAA+AGG + chr4_4:81144613-81144632 None:intergenic 25.0%
! CTCTGATATCAGAAAAATTT+CGG - chr4_4:81144615-81144634 Msa0707730:intron 25.0%
! GGAAAAAAAAAATACATGTC+TGG + chr4_4:81144729-81144748 None:intergenic 25.0%
!!! GCAGTTTTGTTTAGTTTAAA+AGG + chr4_4:81144750-81144769 None:intergenic 25.0%
!! TCATAATGGAAAGAACATAT+TGG + chr4_4:81144998-81145017 None:intergenic 25.0%
! AAAGGCAAAACTTATTATTC+TGG - chr4_4:81144228-81144247 Msa0707730:three_prime_UTR 25.0%
! AAGGAAATAATCAGATACAA+TGG + chr4_4:81144307-81144326 None:intergenic 25.0%
!! ATTTTTCTGATATCAGAGAA+AGG + chr4_4:81144613-81144632 None:intergenic 25.0%
! CTCTGATATCAGAAAAATTT+CGG - chr4_4:81144615-81144634 Msa0707730:intron 25.0%
! GGAAAAAAAAAATACATGTC+TGG + chr4_4:81144729-81144748 None:intergenic 25.0%
!!! GCAGTTTTGTTTAGTTTAAA+AGG + chr4_4:81144750-81144769 None:intergenic 25.0%
!! TCATAATGGAAAGAACATAT+TGG + chr4_4:81144998-81145017 None:intergenic 25.0%
TTGTATTCAACAGGATCAAA+TGG + chr4_4:81144210-81144229 None:intergenic 30.0%
TTTGATCCTGTTGAATACAA+AGG - chr4_4:81144210-81144229 Msa0707730:three_prime_UTR 30.0%
!!! GTGAAGAAAATTTTAGAACC+TGG + chr4_4:81144254-81144273 None:intergenic 30.0%
!! ACGAACATTTGTGTCATTTA+TGG - chr4_4:81144522-81144541 Msa0707730:CDS 30.0%
! TGAAGCTTTTGGATCTATTA+AGG - chr4_4:81144900-81144919 Msa0707730:intron 30.0%
!!! TCTATTAAGGATGTTGCTTT+TGG - chr4_4:81144913-81144932 Msa0707730:intron 30.0%
GAATGACATGAGATGAAATA+AGG + chr4_4:81145024-81145043 None:intergenic 30.0%
!! AACTTTGGTTTGGTTTAACA+TGG - chr4_4:81145118-81145137 Msa0707730:intron 30.0%
TATCAATCCCCATATCATAA+CGG + chr4_4:81145168-81145187 None:intergenic 30.0%
TTGTATTCAACAGGATCAAA+TGG + chr4_4:81144210-81144229 None:intergenic 30.0%
TTTGATCCTGTTGAATACAA+AGG - chr4_4:81144210-81144229 Msa0707730:three_prime_UTR 30.0%
!!! GTGAAGAAAATTTTAGAACC+TGG + chr4_4:81144254-81144273 None:intergenic 30.0%
!! ACGAACATTTGTGTCATTTA+TGG - chr4_4:81144522-81144541 Msa0707730:CDS 30.0%
! TGAAGCTTTTGGATCTATTA+AGG - chr4_4:81144900-81144919 Msa0707730:intron 30.0%
!!! TCTATTAAGGATGTTGCTTT+TGG - chr4_4:81144913-81144932 Msa0707730:intron 30.0%
GAATGACATGAGATGAAATA+AGG + chr4_4:81145024-81145043 None:intergenic 30.0%
!! AACTTTGGTTTGGTTTAACA+TGG - chr4_4:81145118-81145137 Msa0707730:intron 30.0%
TATCAATCCCCATATCATAA+CGG + chr4_4:81145168-81145187 None:intergenic 30.0%
TTTGCTCGTTTCCGAAAAAA+GGG - chr4_4:81144159-81144178 Msa0707730:three_prime_UTR 35.0%
! GTTTTGCCTTTGTATTCAAC+AGG + chr4_4:81144219-81144238 None:intergenic 35.0%
CAAAACTTATTATTCTGGCC+AGG - chr4_4:81144233-81144252 Msa0707730:three_prime_UTR 35.0%
GAAGCATATCATCATGTACT+CGG + chr4_4:81144399-81144418 None:intergenic 35.0%
TTTCTGATATCAGAGAAAGG+TGG + chr4_4:81144610-81144629 None:intergenic 35.0%
TTACAAGTTGCCTAAGGAAA+CGG - chr4_4:81145059-81145078 Msa0707730:intron 35.0%
CAGTTATTTGCTTGCAACTT+TGG - chr4_4:81145103-81145122 Msa0707730:intron 35.0%
ATTTGCTTGCAACTTTGGTT+TGG - chr4_4:81145108-81145127 Msa0707730:intron 35.0%
CTATCTGTACATGGTAAAAG+AGG + chr4_4:81145144-81145163 None:intergenic 35.0%
ACAGATAGACCGTTATGATA+TGG - chr4_4:81145156-81145175 Msa0707730:CDS 35.0%
CAGATAGACCGTTATGATAT+GGG - chr4_4:81145157-81145176 Msa0707730:CDS 35.0%
AGATAGACCGTTATGATATG+GGG - chr4_4:81145158-81145177 Msa0707730:CDS 35.0%
TTTGCTCGTTTCCGAAAAAA+GGG - chr4_4:81144159-81144178 Msa0707730:three_prime_UTR 35.0%
! GTTTTGCCTTTGTATTCAAC+AGG + chr4_4:81144219-81144238 None:intergenic 35.0%
CAAAACTTATTATTCTGGCC+AGG - chr4_4:81144233-81144252 Msa0707730:three_prime_UTR 35.0%
GAAGCATATCATCATGTACT+CGG + chr4_4:81144399-81144418 None:intergenic 35.0%
TTTCTGATATCAGAGAAAGG+TGG + chr4_4:81144610-81144629 None:intergenic 35.0%
TTACAAGTTGCCTAAGGAAA+CGG - chr4_4:81145059-81145078 Msa0707730:intron 35.0%
CAGTTATTTGCTTGCAACTT+TGG - chr4_4:81145103-81145122 Msa0707730:intron 35.0%
ATTTGCTTGCAACTTTGGTT+TGG - chr4_4:81145108-81145127 Msa0707730:intron 35.0%
CTATCTGTACATGGTAAAAG+AGG + chr4_4:81145144-81145163 None:intergenic 35.0%
ACAGATAGACCGTTATGATA+TGG - chr4_4:81145156-81145175 Msa0707730:CDS 35.0%
CAGATAGACCGTTATGATAT+GGG - chr4_4:81145157-81145176 Msa0707730:CDS 35.0%
AGATAGACCGTTATGATATG+GGG - chr4_4:81145158-81145177 Msa0707730:CDS 35.0%
!!! AAATCGCACGACCCTTTTTT+CGG + chr4_4:81144173-81144192 None:intergenic 40.0%
! ATTGTTGGACTGCATTGACA+AGG + chr4_4:81144326-81144345 None:intergenic 40.0%
! TGGGAAGATGTAAGCATTGT+TGG + chr4_4:81144341-81144360 None:intergenic 40.0%
AACAATGCTTACATCTTCCC+AGG - chr4_4:81144340-81144359 Msa0707730:three_prime_UTR 40.0%
! TGGTTTGGGTATAGTGATGT+CGG - chr4_4:81144366-81144385 Msa0707730:three_prime_UTR 40.0%
!! TGTGTCATTTATGGTTGCAG+CGG - chr4_4:81144531-81144550 Msa0707730:CDS 40.0%
ACCGAGGAGAACTACAAAAA+GGG - chr4_4:81144574-81144593 Msa0707730:CDS 40.0%
AGCAACATTTGCAGCTATGT+TGG + chr4_4:81144641-81144660 None:intergenic 40.0%
ACTGCTTCAACTCTCATTGT+AGG - chr4_4:81144765-81144784 Msa0707730:CDS 40.0%
TTCAACTCTCATTGTAGGCT+TGG - chr4_4:81144770-81144789 Msa0707730:CDS 40.0%
GCATACTTGACAAGATTCTC+AGG + chr4_4:81144813-81144832 None:intergenic 40.0%
!!! ACCATTGACTCTGAAGCTTT+TGG - chr4_4:81144889-81144908 Msa0707730:intron 40.0%
TCCAAAAGCTTCAGAGTCAA+TGG + chr4_4:81144893-81144912 None:intergenic 40.0%
!!! AGGATGTTGCTTTTGGTGTA+TGG - chr4_4:81144920-81144939 Msa0707730:intron 40.0%
TGTGTGTTACAAGTTGCCTA+AGG - chr4_4:81145053-81145072 Msa0707730:intron 40.0%
CTTTCTCAAACCGTTTCCTT+AGG + chr4_4:81145072-81145091 None:intergenic 40.0%
CATAACGGTCTATCTGTACA+TGG + chr4_4:81145153-81145172 None:intergenic 40.0%
!!! AAATCGCACGACCCTTTTTT+CGG + chr4_4:81144173-81144192 None:intergenic 40.0%
! ATTGTTGGACTGCATTGACA+AGG + chr4_4:81144326-81144345 None:intergenic 40.0%
! TGGGAAGATGTAAGCATTGT+TGG + chr4_4:81144341-81144360 None:intergenic 40.0%
AACAATGCTTACATCTTCCC+AGG - chr4_4:81144340-81144359 Msa0707730:three_prime_UTR 40.0%
! TGGTTTGGGTATAGTGATGT+CGG - chr4_4:81144366-81144385 Msa0707730:three_prime_UTR 40.0%
!! TGTGTCATTTATGGTTGCAG+CGG - chr4_4:81144531-81144550 Msa0707730:CDS 40.0%
ACCGAGGAGAACTACAAAAA+GGG - chr4_4:81144574-81144593 Msa0707730:CDS 40.0%
AGCAACATTTGCAGCTATGT+TGG + chr4_4:81144641-81144660 None:intergenic 40.0%
ACTGCTTCAACTCTCATTGT+AGG - chr4_4:81144765-81144784 Msa0707730:CDS 40.0%
TTCAACTCTCATTGTAGGCT+TGG - chr4_4:81144770-81144789 Msa0707730:CDS 40.0%
GCATACTTGACAAGATTCTC+AGG + chr4_4:81144813-81144832 None:intergenic 40.0%
!!! ACCATTGACTCTGAAGCTTT+TGG - chr4_4:81144889-81144908 Msa0707730:intron 40.0%
TCCAAAAGCTTCAGAGTCAA+TGG + chr4_4:81144893-81144912 None:intergenic 40.0%
!!! AGGATGTTGCTTTTGGTGTA+TGG - chr4_4:81144920-81144939 Msa0707730:intron 40.0%
TGTGTGTTACAAGTTGCCTA+AGG - chr4_4:81145053-81145072 Msa0707730:intron 40.0%
CTTTCTCAAACCGTTTCCTT+AGG + chr4_4:81145072-81145091 None:intergenic 40.0%
CATAACGGTCTATCTGTACA+TGG + chr4_4:81145153-81145172 None:intergenic 40.0%
! ATCTTCCCAGGCTTTGGTTT+GGG - chr4_4:81144352-81144371 Msa0707730:three_prime_UTR 45.0%
CTATACCCAAACCAAAGCCT+GGG + chr4_4:81144360-81144379 None:intergenic 45.0%
ACTATACCCAAACCAAAGCC+TGG + chr4_4:81144361-81144380 None:intergenic 45.0%
GGTTTGGGTATAGTGATGTC+GGG - chr4_4:81144367-81144386 Msa0707730:three_prime_UTR 45.0%
GACCGAGGAGAACTACAAAA+AGG - chr4_4:81144573-81144592 Msa0707730:CDS 45.0%
! GCCCTTTTTGTAGTTCTCCT+CGG + chr4_4:81144578-81144597 None:intergenic 45.0%
CACACCTAGTTCATATGCCT+TGG + chr4_4:81144665-81144684 None:intergenic 45.0%
TTGACAAGATTCTCAGGACG+AGG + chr4_4:81144807-81144826 None:intergenic 45.0%
ACCTATAGTTGCGGTACAGA+GGG + chr4_4:81144854-81144873 None:intergenic 45.0%
ACCGCAACTATAGGTGAACA+AGG - chr4_4:81144859-81144878 Msa0707730:intron 45.0%
CCCTTGTTCACCTATAGTTG+CGG + chr4_4:81144863-81144882 None:intergenic 45.0%
CCGCAACTATAGGTGAACAA+GGG - chr4_4:81144860-81144879 Msa0707730:intron 45.0%
! ATCTTCCCAGGCTTTGGTTT+GGG - chr4_4:81144352-81144371 Msa0707730:three_prime_UTR 45.0%
CTATACCCAAACCAAAGCCT+GGG + chr4_4:81144360-81144379 None:intergenic 45.0%
ACTATACCCAAACCAAAGCC+TGG + chr4_4:81144361-81144380 None:intergenic 45.0%
GGTTTGGGTATAGTGATGTC+GGG - chr4_4:81144367-81144386 Msa0707730:three_prime_UTR 45.0%
GACCGAGGAGAACTACAAAA+AGG - chr4_4:81144573-81144592 Msa0707730:CDS 45.0%
! GCCCTTTTTGTAGTTCTCCT+CGG + chr4_4:81144578-81144597 None:intergenic 45.0%
CACACCTAGTTCATATGCCT+TGG + chr4_4:81144665-81144684 None:intergenic 45.0%
TTGACAAGATTCTCAGGACG+AGG + chr4_4:81144807-81144826 None:intergenic 45.0%
ACCTATAGTTGCGGTACAGA+GGG + chr4_4:81144854-81144873 None:intergenic 45.0%
ACCGCAACTATAGGTGAACA+AGG - chr4_4:81144859-81144878 Msa0707730:intron 45.0%
CCCTTGTTCACCTATAGTTG+CGG + chr4_4:81144863-81144882 None:intergenic 45.0%
CCGCAACTATAGGTGAACAA+GGG - chr4_4:81144860-81144879 Msa0707730:intron 45.0%
! GTCGTGCGATTTTCGCTAGT+GGG - chr4_4:81144181-81144200 Msa0707730:three_prime_UTR 50.0%
GCTTACATCTTCCCAGGCTT+TGG - chr4_4:81144346-81144365 Msa0707730:three_prime_UTR 50.0%
! CATCTTCCCAGGCTTTGGTT+TGG - chr4_4:81144351-81144370 Msa0707730:three_prime_UTR 50.0%
AGCTGCAAATGTTGCTGCCA+AGG - chr4_4:81144645-81144664 Msa0707730:intron 50.0%
GCTGCCAAGGCATATGAACT+AGG - chr4_4:81144658-81144677 Msa0707730:intron 50.0%
CCTATAGTTGCGGTACAGAG+GGG + chr4_4:81144853-81144872 None:intergenic 50.0%
CACCTATAGTTGCGGTACAG+AGG + chr4_4:81144855-81144874 None:intergenic 50.0%
! GTCGTGCGATTTTCGCTAGT+GGG - chr4_4:81144181-81144200 Msa0707730:three_prime_UTR 50.0%
GCTTACATCTTCCCAGGCTT+TGG - chr4_4:81144346-81144365 Msa0707730:three_prime_UTR 50.0%
! CATCTTCCCAGGCTTTGGTT+TGG - chr4_4:81144351-81144370 Msa0707730:three_prime_UTR 50.0%
AGCTGCAAATGTTGCTGCCA+AGG - chr4_4:81144645-81144664 Msa0707730:intron 50.0%
GCTGCCAAGGCATATGAACT+AGG - chr4_4:81144658-81144677 Msa0707730:intron 50.0%
CCTATAGTTGCGGTACAGAG+GGG + chr4_4:81144853-81144872 None:intergenic 50.0%
CACCTATAGTTGCGGTACAG+AGG + chr4_4:81144855-81144874 None:intergenic 50.0%
! GGTCGTGCGATTTTCGCTAG+TGG - chr4_4:81144180-81144199 Msa0707730:three_prime_UTR 55.0%
TATGGTTGCAGCGGAAGCCT+TGG - chr4_4:81144540-81144559 Msa0707730:CDS 55.0%
CCCCTCTGTACCGCAACTAT+AGG - chr4_4:81144850-81144869 Msa0707730:intron 55.0%
! GGTCGTGCGATTTTCGCTAG+TGG - chr4_4:81144180-81144199 Msa0707730:three_prime_UTR 55.0%
TATGGTTGCAGCGGAAGCCT+TGG - chr4_4:81144540-81144559 Msa0707730:CDS 55.0%
CCCCTCTGTACCGCAACTAT+AGG - chr4_4:81144850-81144869 Msa0707730:intron 55.0%
CTCGGTCACTTGCATCGCCA+AGG + chr4_4:81144560-81144579 None:intergenic 60.0%
CTTGGCGATGCAAGTGACCG+AGG - chr4_4:81144558-81144577 Msa0707730:CDS 60.0%
CTCGGTCACTTGCATCGCCA+AGG + chr4_4:81144560-81144579 None:intergenic 60.0%
CTTGGCGATGCAAGTGACCG+AGG - chr4_4:81144558-81144577 Msa0707730:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 81144159 81145224 81144159 ID=Msa0707730;Name=Msa0707730
chr4_4 mRNA 81144159 81145224 81144159 ID=Msa0707730-mRNA-1;Parent=Msa0707730;Name=Msa0707730-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|1|1|1|1|4|349|134
chr4_4 exon 81144159 81144596 81144159 ID=Msa0707730-mRNA-1:exon:26502;Parent=Msa0707730-mRNA-1
chr4_4 exon 81144704 81144832 81144704 ID=Msa0707730-mRNA-1:exon:26501;Parent=Msa0707730-mRNA-1
chr4_4 exon 81144956 81145046 81144956 ID=Msa0707730-mRNA-1:exon:26500;Parent=Msa0707730-mRNA-1
chr4_4 exon 81145129 81145224 81145129 ID=Msa0707730-mRNA-1:exon:26499;Parent=Msa0707730-mRNA-1
chr4_4 CDS 81145129 81145224 81145129 ID=Msa0707730-mRNA-1:cds;Parent=Msa0707730-mRNA-1
chr4_4 CDS 81144956 81145046 81144956 ID=Msa0707730-mRNA-1:cds;Parent=Msa0707730-mRNA-1
chr4_4 CDS 81144704 81144832 81144704 ID=Msa0707730-mRNA-1:cds;Parent=Msa0707730-mRNA-1
chr4_4 CDS 81144508 81144596 81144508 ID=Msa0707730-mRNA-1:cds;Parent=Msa0707730-mRNA-1
chr4_4 three_prime_UTR 81144159 81144507 81144159 ID=Msa0707730-mRNA-1:three_prime_utr;Parent=Msa0707730-mRNA-1
Gene Sequence

>Msa0707730

TTTGCTCGTTTCCGAAAAAAGGGTCGTGCGATTTTCGCTAGTGGGAGTCCATTTGATCCTGTTGAATACAAAGGCAAAACTTATTATTCTGGCCAGTCCAACAATGCTTACATCTTCCCAGGCTTTGGTTTGGGTATAGTGATGTCGGGAGCAATCCGAGTACATGATGATATGCTTCTAGCAGCCTCGGAAGCCTTGGCGATGCAAGTGACCGAGGAGAACTACAAAAAGGGCTTGACTTATCCACCTTTCTCTGATATCAGAAAAATTTCGGCCAACATAGCTGCAAATGTTGCTGCCAAGGCATATGAACTAGGCTTGGCTACACATCTTCCTCGTCCTGAGAATCTTGTCAAGTATGCTGAGAGTTGCATGTATTCCCCTCTGTACCGCAACTATAGGTGA
Protein sequence

>Msa0707730

FARFRKKGRAIFASGSPFDPVEYKGKTYYSGQSNNAYIFPGFGLGIVMSGAIRVHDDMLLAASEALAMQVTEENYKKGLTYPPFSDIRKISANIAANVAAKAYELGLATHLPRPENLVKYAESCMYSPLYRNYR*