Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0713780 | AES94499.1 | 93.631 | 157 | 10 | 0 | 1 | 157 | 1 | 157 | 3.34e-103 | 302 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0713780 | sp|C0Z2L5|BZP44_ARATH | 35.833 | 120 | 76 | 1 | 31 | 149 | 10 | 129 | 2.82e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0713780 | G7K2E8 | 93.631 | 157 | 10 | 0 | 1 | 157 | 1 | 157 | 1.59e-103 | 302 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0713780 | TF | bZIP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0185790 | Msa0713780 | 0.935039 | 1.584212e-96 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0713780 | MtrunA17_Chr5g0400901 | 93.631 | 157 | 10 | 0 | 1 | 157 | 1 | 157 | 3.07e-107 | 302 |
| Msa0713780 | MtrunA17_Chr4g0054291 | 55.172 | 174 | 61 | 6 | 1 | 157 | 1 | 174 | 3.28e-53 | 166 |
| Msa0713780 | MtrunA17_Chr3g0135061 | 41.085 | 129 | 60 | 3 | 11 | 136 | 16 | 131 | 1.17e-16 | 72.0 |
| Msa0713780 | MtrunA17_Chr1g0152241 | 53.086 | 81 | 38 | 0 | 60 | 140 | 59 | 139 | 2.60e-15 | 68.6 |
| Msa0713780 | MtrunA17_Chr7g0225661 | 40.625 | 96 | 50 | 1 | 58 | 153 | 81 | 169 | 1.16e-14 | 68.2 |
| Msa0713780 | MtrunA17_Chr4g0035414 | 43.210 | 81 | 46 | 0 | 58 | 138 | 27 | 107 | 3.91e-14 | 65.9 |
| Msa0713780 | MtrunA17_Chr8g0341311 | 39.583 | 96 | 51 | 1 | 58 | 153 | 71 | 159 | 1.14e-13 | 65.1 |
| Msa0713780 | MtrunA17_Chr3g0144941 | 37.500 | 96 | 52 | 1 | 58 | 145 | 27 | 122 | 3.64e-12 | 60.5 |
| Msa0713780 | MtrunA17_Chr1g0194681 | 36.667 | 90 | 42 | 2 | 36 | 125 | 8 | 82 | 6.97e-12 | 59.7 |
| Msa0713780 | MtrunA17_Chr1g0155551 | 36.792 | 106 | 63 | 1 | 36 | 137 | 1 | 106 | 1.13e-11 | 59.3 |
| Msa0713780 | MtrunA17_Chr8g0378901 | 42.683 | 82 | 39 | 3 | 59 | 136 | 33 | 110 | 1.16e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0713780 | AT3G49760.1 | 38.129 | 139 | 85 | 1 | 5 | 142 | 15 | 153 | 2.03e-27 | 100 |
| Msa0713780 | AT4G37730.1 | 43.609 | 133 | 60 | 3 | 7 | 124 | 135 | 267 | 3.23e-19 | 82.4 |
| Msa0713780 | AT2G22850.1 | 47.059 | 85 | 45 | 0 | 59 | 143 | 127 | 211 | 3.13e-18 | 78.6 |
| Msa0713780 | AT2G22850.2 | 47.059 | 85 | 45 | 0 | 59 | 143 | 127 | 211 | 3.13e-18 | 78.6 |
| Msa0713780 | AT3G30530.1 | 40.000 | 120 | 67 | 2 | 27 | 146 | 49 | 163 | 4.82e-15 | 68.9 |
| Msa0713780 | AT1G75390.1 | 35.833 | 120 | 76 | 1 | 31 | 149 | 10 | 129 | 2.87e-13 | 64.3 |
| Msa0713780 | AT2G18160.1 | 50.820 | 61 | 23 | 1 | 52 | 105 | 15 | 75 | 9.12e-13 | 62.8 |
| Msa0713780 | AT1G75390.2 | 38.384 | 99 | 60 | 1 | 31 | 128 | 10 | 108 | 2.27e-12 | 60.8 |
| Msa0713780 | AT1G13600.1 | 45.679 | 81 | 37 | 1 | 58 | 138 | 83 | 156 | 4.56e-12 | 61.6 |
| Msa0713780 | AT4G34590.1 | 40.000 | 85 | 51 | 0 | 58 | 142 | 24 | 108 | 4.82e-12 | 60.8 |
| Msa0713780 | AT2G04038.1 | 38.835 | 103 | 51 | 2 | 36 | 138 | 54 | 144 | 1.79e-11 | 59.3 |
| Msa0713780 | AT4G36730.1 | 33.628 | 113 | 56 | 2 | 48 | 141 | 194 | 306 | 1.85e-11 | 61.2 |
| Msa0713780 | AT4G36730.2 | 33.628 | 113 | 56 | 2 | 48 | 141 | 192 | 304 | 2.05e-11 | 60.8 |
| Msa0713780 | AT5G15830.1 | 47.945 | 73 | 32 | 1 | 33 | 105 | 52 | 118 | 5.36e-11 | 58.5 |
Find 52 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGACATCTAGAGAATTTA+CGG | 0.286559 | 5_1:-6221164 | Msa0713780:CDS |
| GGATGGCTCGAACCGGAATA+TGG | 0.309967 | 5_1:-6221253 | Msa0713780:CDS |
| GGGCTGGTGGGTTGGAAGGG+TGG | 0.313437 | 5_1:+6221327 | None:intergenic |
| ATATCATTCTCCTCCGCTTT+CGG | 0.329484 | 5_1:+6221226 | None:intergenic |
| GCAACCCGTTGTTCAATTCC+CGG | 0.358900 | 5_1:+6221112 | None:intergenic |
| CAACCGGAGCCACTCACTTT+CGG | 0.360505 | 5_1:+6221056 | None:intergenic |
| GTGACCTCCGTGCCGATTCC+CGG | 0.363167 | 5_1:+6221199 | None:intergenic |
| TTCTTCTAACTCCGTGAAAT+CGG | 0.384933 | 5_1:+6221383 | None:intergenic |
| GCTTTCGGTCCTCCATATTC+CGG | 0.399400 | 5_1:+6221241 | None:intergenic |
| CTTAGGGCTGGTGGGTTGGA+AGG | 0.408872 | 5_1:+6221323 | None:intergenic |
| ACCGGTTTAGAGTTGAAAAC+CGG | 0.409929 | 5_1:-6221131 | Msa0713780:CDS |
| CCGGTTGGAACGAACCATGC+TGG | 0.411515 | 5_1:-6221040 | Msa0713780:CDS |
| TCTCTATGCCTCCGATTTCA+CGG | 0.417032 | 5_1:-6221394 | Msa0713780:CDS |
| CTCACTTCAAGCTCCGGTTC+AGG | 0.426863 | 5_1:-6221300 | Msa0713780:CDS |
| CCGGAGCTTGAAGTGAGCTT+AGG | 0.429061 | 5_1:+6221306 | None:intergenic |
| AAAACCGGGAATTGAACAAC+GGG | 0.452979 | 5_1:-6221116 | Msa0713780:CDS |
| ATTTACGGAACCAGGTGAAC+CGG | 0.455271 | 5_1:-6221149 | Msa0713780:CDS |
| CCAGCATGGTTCGTTCCAAC+CGG | 0.461195 | 5_1:+6221040 | None:intergenic |
| TGAAGTGAGCTTAGGGCTGG+TGG | 0.471435 | 5_1:+6221314 | None:intergenic |
| CAACTCTAAACCGGTTCACC+TGG | 0.478647 | 5_1:+6221139 | None:intergenic |
| CGGAGCTTGAAGTGAGCTTA+GGG | 0.479442 | 5_1:+6221307 | None:intergenic |
| TTAGGGCTGGTGGGTTGGAA+GGG | 0.484445 | 5_1:+6221324 | None:intergenic |
| CGGTCATGGATGGCTCGAAC+CGG | 0.489104 | 5_1:-6221260 | Msa0713780:CDS |
| CGGTTCGAGCCATCCATGAC+CGG | 0.490515 | 5_1:+6221261 | None:intergenic |
| TTCAACCTTTCTCATCTGCA+TGG | 0.491200 | 5_1:-6220981 | Msa0713780:CDS |
| GGAGGAGAATGATATCAAAC+CGG | 0.491685 | 5_1:-6221218 | Msa0713780:CDS |
| AATGATATCAAACCGGGAAT+CGG | 0.493933 | 5_1:-6221211 | Msa0713780:CDS |
| GAACCGAAAGTGAGTGGCTC+CGG | 0.506432 | 5_1:-6221059 | Msa0713780:CDS |
| TGAGCTTAGGGCTGGTGGGT+TGG | 0.515513 | 5_1:+6221319 | None:intergenic |
| CCTAAGCTCACTTCAAGCTC+CGG | 0.518730 | 5_1:-6221306 | Msa0713780:CDS |
| CGGTTGGAACGAACCATGCT+GGG | 0.526524 | 5_1:-6221039 | Msa0713780:CDS |
| GAAGTGAGCTTAGGGCTGGT+GGG | 0.547493 | 5_1:+6221315 | None:intergenic |
| GGCATAGAGAGAAGAGAGCA+TGG | 0.571054 | 5_1:+6221407 | None:intergenic |
| CATGGCCATGCAGATGAGAA+AGG | 0.571565 | 5_1:+6220976 | None:intergenic |
| GCTTGAAGTGAGCTTAGGGC+TGG | 0.571567 | 5_1:+6221311 | None:intergenic |
| AGAAGAAAGTTTAACGCCGT+GGG | 0.579656 | 5_1:-6221367 | Msa0713780:CDS |
| CCGGTTTAGAGTTGAAAACC+GGG | 0.582456 | 5_1:-6221130 | Msa0713780:CDS |
| TCTAGAGAATTTACGGAACC+AGG | 0.588686 | 5_1:-6221157 | Msa0713780:CDS |
| TAGAAGAAAGTTTAACGCCG+TGG | 0.593173 | 5_1:-6221368 | Msa0713780:CDS |
| CGAAAGTGAGTGGCTCCGGT+TGG | 0.593366 | 5_1:-6221055 | Msa0713780:CDS |
| TATCAAACCGGGAATCGGCA+CGG | 0.599707 | 5_1:-6221206 | Msa0713780:CDS |
| GTCTACGAACCGAAAGTGAG+TGG | 0.602935 | 5_1:-6221065 | Msa0713780:CDS |
| TTCTAACTCCGTGAAATCGG+AGG | 0.604958 | 5_1:+6221386 | None:intergenic |
| GGTCAAAAGTTGTCAAACAT+AGG | 0.610074 | 5_1:-6221018 | Msa0713780:CDS |
| GGAATATGGAGGACCGAAAG+CGG | 0.611264 | 5_1:-6221239 | Msa0713780:CDS |
| GAGGAGAATGATATCAAACC+GGG | 0.616694 | 5_1:-6221217 | Msa0713780:CDS |
| TCAGCTGTGACAATATTGCA+TGG | 0.636378 | 5_1:+6220958 | None:intergenic |
| GTCAAACATAGGTCAAAACA+TGG | 0.654401 | 5_1:-6221007 | Msa0713780:CDS |
| GAAAACCGGGAATTGAACAA+CGG | 0.656607 | 5_1:-6221117 | Msa0713780:CDS |
| ATATGGAGGACCGAAAGCGG+AGG | 0.670255 | 5_1:-6221236 | Msa0713780:CDS |
| TGGCTCGAACCGGAATATGG+AGG | 0.676236 | 5_1:-6221250 | Msa0713780:CDS |
| CAAACCGGGAATCGGCACGG+AGG | 0.765542 | 5_1:-6221203 | Msa0713780:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGACATCTAGAGAATTTA+CGG | - | chr5_1:6221195-6221214 | Msa0713780:CDS | 25.0% |
| AGAATGTTAGAAAAATCCCA+CGG | + | chr5_1:6221011-6221030 | None:intergenic | 30.0% | |
| TTCTTCTAACTCCGTGAAAT+CGG | + | chr5_1:6220979-6220998 | None:intergenic | 35.0% | |
| AATGATATCAAACCGGGAAT+CGG | - | chr5_1:6221148-6221167 | Msa0713780:CDS | 35.0% | |
| GGTCAAAAGTTGTCAAACAT+AGG | - | chr5_1:6221341-6221360 | Msa0713780:CDS | 35.0% | |
| GTCAAACATAGGTCAAAACA+TGG | - | chr5_1:6221352-6221371 | Msa0713780:CDS | 35.0% | |
| TAGAAGAAAGTTTAACGCCG+TGG | - | chr5_1:6220991-6221010 | Msa0713780:CDS | 40.0% | |
| AGAAGAAAGTTTAACGCCGT+GGG | - | chr5_1:6220992-6221011 | Msa0713780:CDS | 40.0% | |
| AGGCGAACCAAAAGAAAAAC+CGG | - | chr5_1:6221079-6221098 | Msa0713780:CDS | 40.0% | |
| ACCAAAAGAAAAACCGGTCA+TGG | - | chr5_1:6221085-6221104 | Msa0713780:CDS | 40.0% | |
| !! | TCCATGACCGGTTTTTCTTT+TGG | + | chr5_1:6221089-6221108 | None:intergenic | 40.0% |
| AAAGAAAAACCGGTCATGGA+TGG | - | chr5_1:6221089-6221108 | Msa0713780:CDS | 40.0% | |
| ATATCATTCTCCTCCGCTTT+CGG | + | chr5_1:6221136-6221155 | None:intergenic | 40.0% | |
| GGAGGAGAATGATATCAAAC+CGG | - | chr5_1:6221141-6221160 | Msa0713780:CDS | 40.0% | |
| GAGGAGAATGATATCAAACC+GGG | - | chr5_1:6221142-6221161 | Msa0713780:CDS | 40.0% | |
| TCTAGAGAATTTACGGAACC+AGG | - | chr5_1:6221202-6221221 | Msa0713780:CDS | 40.0% | |
| ! | ACCGGTTTAGAGTTGAAAAC+CGG | - | chr5_1:6221228-6221247 | Msa0713780:CDS | 40.0% |
| GAAAACCGGGAATTGAACAA+CGG | - | chr5_1:6221242-6221261 | Msa0713780:CDS | 40.0% | |
| AAAACCGGGAATTGAACAAC+GGG | - | chr5_1:6221243-6221262 | Msa0713780:CDS | 40.0% | |
| TTCAACCTTTCTCATCTGCA+TGG | - | chr5_1:6221378-6221397 | Msa0713780:CDS | 40.0% | |
| TCAGCTGTGACAATATTGCA+TGG | + | chr5_1:6221404-6221423 | None:intergenic | 40.0% | |
| TCTCTATGCCTCCGATTTCA+CGG | - | chr5_1:6220965-6220984 | Msa0713780:CDS | 45.0% | |
| TTCTAACTCCGTGAAATCGG+AGG | + | chr5_1:6220976-6220995 | None:intergenic | 45.0% | |
| !!! | TTCTTTTGGTTCGCCTGAAC+CGG | + | chr5_1:6221075-6221094 | None:intergenic | 45.0% |
| ATTTACGGAACCAGGTGAAC+CGG | - | chr5_1:6221210-6221229 | Msa0713780:CDS | 45.0% | |
| ! | CCCGGTTTTCAACTCTAAAC+CGG | + | chr5_1:6221232-6221251 | None:intergenic | 45.0% |
| ! | CCGGTTTAGAGTTGAAAACC+GGG | - | chr5_1:6221229-6221248 | Msa0713780:CDS | 45.0% |
| ! | TGACAACTTTTGACCCAGCA+TGG | + | chr5_1:6221336-6221355 | None:intergenic | 45.0% |
| CGGAGCTTGAAGTGAGCTTA+GGG | + | chr5_1:6221055-6221074 | None:intergenic | 50.0% | |
| CCTAAGCTCACTTCAAGCTC+CGG | - | chr5_1:6221053-6221072 | Msa0713780:CDS | 50.0% | |
| GCTTTCGGTCCTCCATATTC+CGG | + | chr5_1:6221121-6221140 | None:intergenic | 50.0% | |
| GGAATATGGAGGACCGAAAG+CGG | - | chr5_1:6221120-6221139 | Msa0713780:CDS | 50.0% | |
| TATCAAACCGGGAATCGGCA+CGG | - | chr5_1:6221153-6221172 | Msa0713780:CDS | 50.0% | |
| ! | CAACTCTAAACCGGTTCACC+TGG | + | chr5_1:6221223-6221242 | None:intergenic | 50.0% |
| GCAACCCGTTGTTCAATTCC+CGG | + | chr5_1:6221250-6221269 | None:intergenic | 50.0% | |
| GTCTACGAACCGAAAGTGAG+TGG | - | chr5_1:6221294-6221313 | Msa0713780:CDS | 50.0% | |
| CATGGCCATGCAGATGAGAA+AGG | + | chr5_1:6221386-6221405 | None:intergenic | 50.0% | |
| !! | TTAGGGCTGGTGGGTTGGAA+GGG | + | chr5_1:6221038-6221057 | None:intergenic | 55.0% |
| ! | GAAGTGAGCTTAGGGCTGGT+GGG | + | chr5_1:6221047-6221066 | None:intergenic | 55.0% |
| TGAAGTGAGCTTAGGGCTGG+TGG | + | chr5_1:6221048-6221067 | None:intergenic | 55.0% | |
| GCTTGAAGTGAGCTTAGGGC+TGG | + | chr5_1:6221051-6221070 | None:intergenic | 55.0% | |
| CCGGAGCTTGAAGTGAGCTT+AGG | + | chr5_1:6221056-6221075 | None:intergenic | 55.0% | |
| CTCACTTCAAGCTCCGGTTC+AGG | - | chr5_1:6221059-6221078 | Msa0713780:CDS | 55.0% | |
| GGATGGCTCGAACCGGAATA+TGG | - | chr5_1:6221106-6221125 | Msa0713780:CDS | 55.0% | |
| TGGCTCGAACCGGAATATGG+AGG | - | chr5_1:6221109-6221128 | Msa0713780:CDS | 55.0% | |
| ATATGGAGGACCGAAAGCGG+AGG | - | chr5_1:6221123-6221142 | Msa0713780:CDS | 55.0% | |
| GAACCGAAAGTGAGTGGCTC+CGG | - | chr5_1:6221300-6221319 | Msa0713780:CDS | 55.0% | |
| CAACCGGAGCCACTCACTTT+CGG | + | chr5_1:6221306-6221325 | None:intergenic | 55.0% | |
| CCAGCATGGTTCGTTCCAAC+CGG | + | chr5_1:6221322-6221341 | None:intergenic | 55.0% | |
| CGGTTGGAACGAACCATGCT+GGG | - | chr5_1:6221320-6221339 | Msa0713780:CDS | 55.0% | |
| !! | CTTAGGGCTGGTGGGTTGGA+AGG | + | chr5_1:6221039-6221058 | None:intergenic | 60.0% |
| ! | TGAGCTTAGGGCTGGTGGGT+TGG | + | chr5_1:6221043-6221062 | None:intergenic | 60.0% |
| CGGTTCGAGCCATCCATGAC+CGG | + | chr5_1:6221101-6221120 | None:intergenic | 60.0% | |
| CGGTCATGGATGGCTCGAAC+CGG | - | chr5_1:6221099-6221118 | Msa0713780:CDS | 60.0% | |
| CGAAAGTGAGTGGCTCCGGT+TGG | - | chr5_1:6221304-6221323 | Msa0713780:CDS | 60.0% | |
| CCGGTTGGAACGAACCATGC+TGG | - | chr5_1:6221319-6221338 | Msa0713780:CDS | 60.0% | |
| CAAACCGGGAATCGGCACGG+AGG | - | chr5_1:6221156-6221175 | Msa0713780:CDS | 65.0% | |
| !! | GTGACCTCCGTGCCGATTCC+CGG | + | chr5_1:6221163-6221182 | None:intergenic | 65.0% |
| !! | GGGCTGGTGGGTTGGAAGGG+TGG | + | chr5_1:6221035-6221054 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 6220954 | 6221427 | 6220954 | ID=Msa0713780;Name=Msa0713780 |
| chr5_1 | mRNA | 6220954 | 6221427 | 6220954 | ID=Msa0713780-mRNA-1;Parent=Msa0713780;Name=Msa0713780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|157 |
| chr5_1 | exon | 6220954 | 6221427 | 6220954 | ID=Msa0713780-mRNA-1:exon:2647;Parent=Msa0713780-mRNA-1 |
| chr5_1 | CDS | 6220954 | 6221427 | 6220954 | ID=Msa0713780-mRNA-1:cds;Parent=Msa0713780-mRNA-1 |
| Gene Sequence |
| Protein sequence |
