AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0715170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715170 MtrunA17_Chr5g0402391 94.545 110 5 1 1 110 1 109 8.70e-70 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGTGTATAGATTTAATATT+TGG 0.143028 5_1:-8091657 Msa0715170:three_prime_UTR
GGGATGTGGTGGGTTATTTA+TGG 0.174091 5_1:-8091636 Msa0715170:three_prime_UTR
AATGTTATTGTTAACGTTCA+TGG 0.256129 5_1:+8091819 None:intergenic
TCGTTGGTTCGTTCTTTGAA+TGG 0.337118 5_1:-8091591 Msa0715170:three_prime_UTR
GAGATTTCTTCTCAGGTTGT+AGG 0.348310 5_1:-8092864 Msa0715170:CDS
CTTCTCAGGTTGTAGGTGTC+AGG 0.365163 5_1:-8092857 Msa0715170:CDS
ACTGCGTTTGCGTTTGCGTT+TGG 0.366463 5_1:+8093345 None:intergenic
ATCAATGGAGATTTCTTCTC+AGG 0.379055 5_1:-8092871 Msa0715170:exon
CGTTGGTTCGTTCTTTGAAT+GGG 0.382563 5_1:-8091590 Msa0715170:three_prime_UTR
GACACGTCGTTGTAACTAAC+GGG 0.389369 5_1:+8093242 None:intergenic
TTTGAAATTTGAATTTGCTT+TGG 0.389650 5_1:-8092357 Msa0715170:CDS
AGTGTATAGATTTAATATTT+GGG 0.397091 5_1:-8091656 Msa0715170:three_prime_UTR
GGATCCATTGCTTGAACAAC+AGG 0.405756 5_1:+8092662 None:intergenic
TTCAAACGAGTTTGAGTCAA+TGG 0.407914 5_1:-8092100 Msa0715170:three_prime_UTR
TCGCTGAATTTCATCATCAA+TGG 0.423818 5_1:-8092886 Msa0715170:exon
GGAAAATTATGAAGGGATCA+TGG 0.430084 5_1:+8093437 None:intergenic
TTGCAAGTGGAAAATTATGA+AGG 0.432757 5_1:+8093429 None:intergenic
AGAAATTGAACGGTTAGGAT+TGG 0.454247 5_1:+8093110 None:intergenic
TCCCTTGTTTAATCAGATAA+CGG 0.456647 5_1:-8093217 Msa0715170:five_prime_UTR
GAAAATTATGAAGGGATCAT+GGG 0.462110 5_1:+8093438 None:intergenic
ATAGGAATGGGAAGAGGATG+AGG 0.467161 5_1:+8093297 None:intergenic
ACCGACGATTCTTCCGCAAC+CGG 0.471136 5_1:-8092736 Msa0715170:CDS
TGATAAGAAATTGAACGGTT+AGG 0.471693 5_1:+8093105 None:intergenic
AAAGATTAAGGTAGTTTCGT+TGG 0.472995 5_1:-8091607 Msa0715170:three_prime_UTR
ATTTAATATTTGGGATGTGG+TGG 0.487137 5_1:-8091647 Msa0715170:three_prime_UTR
CAGTCTCCGTTAATAGTGAC+GGG 0.487784 5_1:+8093165 None:intergenic
CGCTCAAGCTGCATTAGCCA+TGG 0.498701 5_1:-8092829 Msa0715170:CDS
GTTGGAATAGGAATGGGAAG+AGG 0.501706 5_1:+8093291 None:intergenic
CGCCGTTATCTGATTAAACA+AGG 0.505496 5_1:+8093215 None:intergenic
GATGAAATTCAGCGAAGTAC+GGG 0.509147 5_1:+8092894 None:intergenic
TCTCAAAACGGGGAATTTGA+CGG 0.510894 5_1:+8093192 None:intergenic
TTGATAACGCGCGTGGAGTA+CGG 0.517751 5_1:+8093063 None:intergenic
CACTCGTAGCGTCCTTCCAA+TGG 0.522068 5_1:+8091855 None:intergenic
CCAGTCTCCGTTAATAGTGA+CGG 0.523295 5_1:+8093164 None:intergenic
TAGATTTAATATTTGGGATG+TGG 0.523492 5_1:-8091650 Msa0715170:three_prime_UTR
TGATGAAATTCAGCGAAGTA+CGG 0.524846 5_1:+8092893 None:intergenic
CCGTTCAACTCGATGAAGAA+AGG 0.525470 5_1:-8092641 Msa0715170:CDS
TTTAATATTTGGGATGTGGT+GGG 0.527047 5_1:-8091646 Msa0715170:three_prime_UTR
CCTTTCTTCATCGAGTTGAA+CGG 0.528328 5_1:+8092641 None:intergenic
CCGTCACTATTAACGGAGAC+TGG 0.530529 5_1:-8093164 Msa0715170:five_prime_UTR
GTGAAGGATGTTCCATTGGA+AGG 0.536178 5_1:-8091867 Msa0715170:three_prime_UTR
TTTCTATTCACACCTCACTC+TGG 0.540409 5_1:+8092938 None:intergenic
TCTTCAAGCTCAAATTCCGT+TGG 0.553314 5_1:+8092036 None:intergenic
GCGTCCTGTTGTTCAAGCAA+TGG 0.555643 5_1:-8092666 Msa0715170:CDS
TGCAAGTGGAAAATTATGAA+GGG 0.556850 5_1:+8093430 None:intergenic
TATTGTGAAGGATGTTCCAT+TGG 0.578054 5_1:-8091871 Msa0715170:three_prime_UTR
ACCGGTTGCGGAAGAATCGT+CGG 0.583673 5_1:+8092735 None:intergenic
TTGGAATTTACATACAACAA+AGG 0.586695 5_1:+8093394 None:intergenic
GATCAAATCGTTAGATCTAG+AGG 0.589450 5_1:-8092619 Msa0715170:intron
TGACACGTCGTTGTAACTAA+CGG 0.593876 5_1:+8093241 None:intergenic
GAAACGCGATTATTTGCAAG+TGG 0.593999 5_1:+8093416 None:intergenic
CATTGGAAGGACGCTACGAG+TGG 0.598974 5_1:-8091854 Msa0715170:three_prime_UTR
TTCTTCATCGGAGATATCAC+CGG 0.604193 5_1:+8092695 None:intergenic
TGAAGTTGTAGCTTCATCCA+TGG 0.608270 5_1:+8092812 None:intergenic
ATTGCTTGAACAACAGGACG+CGG 0.618562 5_1:+8092668 None:intergenic
ACAGTGAAGAAGAATTTCAG+AGG 0.627175 5_1:-8092382 Msa0715170:CDS
GGACGCGGGAAATTCTTCAT+CGG 0.641501 5_1:+8092683 None:intergenic
GAGAGTGTTGATAACGCGCG+TGG 0.646351 5_1:+8093056 None:intergenic
TGTGATGATAAGAAATTGAA+CGG 0.647986 5_1:+8093100 None:intergenic
TTGCTTGAACAACAGGACGC+GGG 0.649814 5_1:+8092669 None:intergenic
TTCATCGGAGATATCACCGG+AGG 0.654993 5_1:+8092698 None:intergenic
ATTGGAAGGACGCTACGAGT+GGG 0.655621 5_1:-8091853 Msa0715170:three_prime_UTR
ACACCTGAAAAGCCAGAGTG+AGG 0.658166 5_1:-8092950 Msa0715170:five_prime_UTR
GTATAATTATGATATTGTGA+AGG 0.659384 5_1:-8091883 Msa0715170:three_prime_UTR
AAAATTATGAAGGGATCATG+GGG 0.661542 5_1:+8093439 None:intergenic
GTCTCCGTTAATAGTGACGG+GGG 0.678897 5_1:+8093167 None:intergenic
ACACGTCGTTGTAACTAACG+GGG 0.680569 5_1:+8093243 None:intergenic
GCCGTTATCTGATTAAACAA+GGG 0.689324 5_1:+8093216 None:intergenic
AGTCTCCGTTAATAGTGACG+GGG 0.697219 5_1:+8093166 None:intergenic
CACGTCGTTGTAACTAACGG+GGG 0.714208 5_1:+8093244 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTACTATTTTAGTTT+AGG - chr5_1:8093254-8093273 Msa0715170:five_prime_UTR 15.0%
!! TAGTGTATAGATTTAATATT+TGG - chr5_1:8093340-8093359 Msa0715170:five_prime_UTR 15.0%
!! AGTGTATAGATTTAATATTT+GGG - chr5_1:8093341-8093360 Msa0715170:five_prime_UTR 15.0%
!!! TTTTCTTACTATTTTAGTTT+AGG - chr5_1:8093254-8093273 Msa0715170:five_prime_UTR 15.0%
!! TAGTGTATAGATTTAATATT+TGG - chr5_1:8093340-8093359 Msa0715170:five_prime_UTR 15.0%
!! AGTGTATAGATTTAATATTT+GGG - chr5_1:8093341-8093360 Msa0715170:five_prime_UTR 15.0%
!!! TTTGAAATTTGAATTTGCTT+TGG - chr5_1:8092640-8092659 Msa0715170:CDS 20.0%
!!! TTAGTTATAGTTTTTGAGTT+AGG - chr5_1:8092673-8092692 Msa0715170:CDS 20.0%
!!! ATATTTTTTGGAGAAGAAAT+CGG + chr5_1:8092940-8092959 None:intergenic 20.0%
!! GTATAATTATGATATTGTGA+AGG - chr5_1:8093114-8093133 Msa0715170:five_prime_UTR 20.0%
!! TTATGGAAGAAAAAAGATTA+AGG - chr5_1:8093378-8093397 Msa0715170:five_prime_UTR 20.0%
!!! TTTGAAATTTGAATTTGCTT+TGG - chr5_1:8092640-8092659 Msa0715170:CDS 20.0%
!!! TTAGTTATAGTTTTTGAGTT+AGG - chr5_1:8092673-8092692 Msa0715170:CDS 20.0%
!!! ATATTTTTTGGAGAAGAAAT+CGG + chr5_1:8092940-8092959 None:intergenic 20.0%
!! GTATAATTATGATATTGTGA+AGG - chr5_1:8093114-8093133 Msa0715170:five_prime_UTR 20.0%
!! TTATGGAAGAAAAAAGATTA+AGG - chr5_1:8093378-8093397 Msa0715170:five_prime_UTR 20.0%
! TTGGAATTTACATACAACAA+AGG + chr5_1:8091606-8091625 None:intergenic 25.0%
! TGTGATGATAAGAAATTGAA+CGG + chr5_1:8091900-8091919 None:intergenic 25.0%
!!! TTTCTTCTCTTTGAAGTTTT+TGG + chr5_1:8092214-8092233 None:intergenic 25.0%
! CTGAAATGAACAATTTAATG+CGG - chr5_1:8092430-8092449 Msa0715170:intron 25.0%
! CATTAAACGTTTCTATTTAC+AGG - chr5_1:8092556-8092575 Msa0715170:intron 25.0%
!! ATTTTGAACGGAAAAAATTG+TGG + chr5_1:8092746-8092765 None:intergenic 25.0%
!!! TATTTTTTGGAGAAGAAATC+GGG + chr5_1:8092939-8092958 None:intergenic 25.0%
!! CTTTTTCTGAATGTTTTTCT+CGG + chr5_1:8093001-8093020 None:intergenic 25.0%
! AATGTTATTGTTAACGTTCA+TGG + chr5_1:8093181-8093200 None:intergenic 25.0%
!! TAGATTTAATATTTGGGATG+TGG - chr5_1:8093347-8093366 Msa0715170:five_prime_UTR 25.0%
! AATCATCATCATCATGTATT+TGG + chr5_1:8091525-8091544 None:intergenic 25.0%
! TTGGAATTTACATACAACAA+AGG + chr5_1:8091606-8091625 None:intergenic 25.0%
! TGTGATGATAAGAAATTGAA+CGG + chr5_1:8091900-8091919 None:intergenic 25.0%
!!! TTTCTTCTCTTTGAAGTTTT+TGG + chr5_1:8092214-8092233 None:intergenic 25.0%
! CTGAAATGAACAATTTAATG+CGG - chr5_1:8092430-8092449 Msa0715170:intron 25.0%
! CATTAAACGTTTCTATTTAC+AGG - chr5_1:8092556-8092575 Msa0715170:intron 25.0%
!! ATTTTGAACGGAAAAAATTG+TGG + chr5_1:8092746-8092765 None:intergenic 25.0%
!!! TATTTTTTGGAGAAGAAATC+GGG + chr5_1:8092939-8092958 None:intergenic 25.0%
!! CTTTTTCTGAATGTTTTTCT+CGG + chr5_1:8093001-8093020 None:intergenic 25.0%
! AATGTTATTGTTAACGTTCA+TGG + chr5_1:8093181-8093200 None:intergenic 25.0%
!! TAGATTTAATATTTGGGATG+TGG - chr5_1:8093347-8093366 Msa0715170:five_prime_UTR 25.0%
AAAATTATGAAGGGATCATG+GGG + chr5_1:8091561-8091580 None:intergenic 30.0%
GAAAATTATGAAGGGATCAT+GGG + chr5_1:8091562-8091581 None:intergenic 30.0%
TGCAAGTGGAAAATTATGAA+GGG + chr5_1:8091570-8091589 None:intergenic 30.0%
TTGCAAGTGGAAAATTATGA+AGG + chr5_1:8091571-8091590 None:intergenic 30.0%
!! TGTTTTGTTGGAATAGGAAT+GGG + chr5_1:8091715-8091734 None:intergenic 30.0%
! TCCCTTGTTTAATCAGATAA+CGG - chr5_1:8091780-8091799 Msa0715170:three_prime_UTR 30.0%
TGATAAGAAATTGAACGGTT+AGG + chr5_1:8091895-8091914 None:intergenic 30.0%
! GAGGATTTTTCATCTTTTAC+CGG + chr5_1:8092283-8092302 None:intergenic 30.0%
GTAAAAGATGAAAAATCCTC+CGG - chr5_1:8092283-8092302 Msa0715170:three_prime_UTR 30.0%
ATAGAAACGTTTAATGCGTT+AGG + chr5_1:8092552-8092571 None:intergenic 30.0%
TAAACGTTTCTATTTACAGG+TGG - chr5_1:8092559-8092578 Msa0715170:intron 30.0%
! TCAGTTACTACGATTTTGAA+CGG + chr5_1:8092758-8092777 None:intergenic 30.0%
! ACAGAATATTTTGTGAGTGT+AGG - chr5_1:8092860-8092879 Msa0715170:CDS 30.0%
TTCTCCAAAAAATATGCCAA+CGG - chr5_1:8092945-8092964 Msa0715170:five_prime_UTR 30.0%
!!! AATTCCGTTGGCATATTTTT+TGG + chr5_1:8092952-8092971 None:intergenic 30.0%
! TTCTTGAAGAATGATTTTGC+AGG - chr5_1:8093092-8093111 Msa0715170:five_prime_UTR 30.0%
AAGAAAAAATGTCAGCAAAG+TGG + chr5_1:8093241-8093260 None:intergenic 30.0%
! ATTTAATATTTGGGATGTGG+TGG - chr5_1:8093350-8093369 Msa0715170:five_prime_UTR 30.0%
! TTTAATATTTGGGATGTGGT+GGG - chr5_1:8093351-8093370 Msa0715170:five_prime_UTR 30.0%
AAAGATTAAGGTAGTTTCGT+TGG - chr5_1:8093390-8093409 Msa0715170:five_prime_UTR 30.0%
AAAATTATGAAGGGATCATG+GGG + chr5_1:8091561-8091580 None:intergenic 30.0%
GAAAATTATGAAGGGATCAT+GGG + chr5_1:8091562-8091581 None:intergenic 30.0%
TGCAAGTGGAAAATTATGAA+GGG + chr5_1:8091570-8091589 None:intergenic 30.0%
TTGCAAGTGGAAAATTATGA+AGG + chr5_1:8091571-8091590 None:intergenic 30.0%
!! TGTTTTGTTGGAATAGGAAT+GGG + chr5_1:8091715-8091734 None:intergenic 30.0%
! TCCCTTGTTTAATCAGATAA+CGG - chr5_1:8091780-8091799 Msa0715170:three_prime_UTR 30.0%
TGATAAGAAATTGAACGGTT+AGG + chr5_1:8091895-8091914 None:intergenic 30.0%
! GAGGATTTTTCATCTTTTAC+CGG + chr5_1:8092283-8092302 None:intergenic 30.0%
GTAAAAGATGAAAAATCCTC+CGG - chr5_1:8092283-8092302 Msa0715170:three_prime_UTR 30.0%
ATAGAAACGTTTAATGCGTT+AGG + chr5_1:8092552-8092571 None:intergenic 30.0%
TAAACGTTTCTATTTACAGG+TGG - chr5_1:8092559-8092578 Msa0715170:intron 30.0%
! TCAGTTACTACGATTTTGAA+CGG + chr5_1:8092758-8092777 None:intergenic 30.0%
! ACAGAATATTTTGTGAGTGT+AGG - chr5_1:8092860-8092879 Msa0715170:CDS 30.0%
TTCTCCAAAAAATATGCCAA+CGG - chr5_1:8092945-8092964 Msa0715170:five_prime_UTR 30.0%
!!! AATTCCGTTGGCATATTTTT+TGG + chr5_1:8092952-8092971 None:intergenic 30.0%
! TTCTTGAAGAATGATTTTGC+AGG - chr5_1:8093092-8093111 Msa0715170:five_prime_UTR 30.0%
AAGAAAAAATGTCAGCAAAG+TGG + chr5_1:8093241-8093260 None:intergenic 30.0%
! ATTTAATATTTGGGATGTGG+TGG - chr5_1:8093350-8093369 Msa0715170:five_prime_UTR 30.0%
! TTTAATATTTGGGATGTGGT+GGG - chr5_1:8093351-8093370 Msa0715170:five_prime_UTR 30.0%
AAAGATTAAGGTAGTTTCGT+TGG - chr5_1:8093390-8093409 Msa0715170:five_prime_UTR 30.0%
GGAAAATTATGAAGGGATCA+TGG + chr5_1:8091563-8091582 None:intergenic 35.0%
!! TTTTGAGTGAAAAGTGCGTT+TGG + chr5_1:8091625-8091644 None:intergenic 35.0%
!! GTGTTTTGTTGGAATAGGAA+TGG + chr5_1:8091716-8091735 None:intergenic 35.0%
!! AAACGGTGTTTTGTTGGAAT+AGG + chr5_1:8091721-8091740 None:intergenic 35.0%
GCCGTTATCTGATTAAACAA+GGG + chr5_1:8091784-8091803 None:intergenic 35.0%
AGAAATTGAACGGTTAGGAT+TGG + chr5_1:8091890-8091909 None:intergenic 35.0%
!!! TGCTTTTTCTGCGATTTTTC+TGG + chr5_1:8092022-8092041 None:intergenic 35.0%
TGATGAAATTCAGCGAAGTA+CGG + chr5_1:8092107-8092126 None:intergenic 35.0%
TCGCTGAATTTCATCATCAA+TGG - chr5_1:8092111-8092130 Msa0715170:three_prime_UTR 35.0%
ATCAATGGAGATTTCTTCTC+AGG - chr5_1:8092126-8092145 Msa0715170:three_prime_UTR 35.0%
!! TTTTCATCTTTTACCGGTTG+CGG + chr5_1:8092277-8092296 None:intergenic 35.0%
GATCAAATCGTTAGATCTAG+AGG - chr5_1:8092378-8092397 Msa0715170:CDS 35.0%
ACAGTGAAGAAGAATTTCAG+AGG - chr5_1:8092615-8092634 Msa0715170:intron 35.0%
TTCAAACGAGTTTGAGTCAA+TGG - chr5_1:8092897-8092916 Msa0715170:five_prime_UTR 35.0%
TATTGTGAAGGATGTTCCAT+TGG - chr5_1:8093126-8093145 Msa0715170:five_prime_UTR 35.0%
GGAAAATTATGAAGGGATCA+TGG + chr5_1:8091563-8091582 None:intergenic 35.0%
!! TTTTGAGTGAAAAGTGCGTT+TGG + chr5_1:8091625-8091644 None:intergenic 35.0%
!! GTGTTTTGTTGGAATAGGAA+TGG + chr5_1:8091716-8091735 None:intergenic 35.0%
!! AAACGGTGTTTTGTTGGAAT+AGG + chr5_1:8091721-8091740 None:intergenic 35.0%
GCCGTTATCTGATTAAACAA+GGG + chr5_1:8091784-8091803 None:intergenic 35.0%
AGAAATTGAACGGTTAGGAT+TGG + chr5_1:8091890-8091909 None:intergenic 35.0%
!!! TGCTTTTTCTGCGATTTTTC+TGG + chr5_1:8092022-8092041 None:intergenic 35.0%
TGATGAAATTCAGCGAAGTA+CGG + chr5_1:8092107-8092126 None:intergenic 35.0%
TCGCTGAATTTCATCATCAA+TGG - chr5_1:8092111-8092130 Msa0715170:three_prime_UTR 35.0%
ATCAATGGAGATTTCTTCTC+AGG - chr5_1:8092126-8092145 Msa0715170:three_prime_UTR 35.0%
!! TTTTCATCTTTTACCGGTTG+CGG + chr5_1:8092277-8092296 None:intergenic 35.0%
GATCAAATCGTTAGATCTAG+AGG - chr5_1:8092378-8092397 Msa0715170:CDS 35.0%
ACAGTGAAGAAGAATTTCAG+AGG - chr5_1:8092615-8092634 Msa0715170:intron 35.0%
TTCAAACGAGTTTGAGTCAA+TGG - chr5_1:8092897-8092916 Msa0715170:five_prime_UTR 35.0%
TATTGTGAAGGATGTTCCAT+TGG - chr5_1:8093126-8093145 Msa0715170:five_prime_UTR 35.0%
GAAACGCGATTATTTGCAAG+TGG + chr5_1:8091584-8091603 None:intergenic 40.0%
TAACTAACGGGGGATGAAAA+CGG + chr5_1:8091746-8091765 None:intergenic 40.0%
TGACACGTCGTTGTAACTAA+CGG + chr5_1:8091759-8091778 None:intergenic 40.0%
CGCCGTTATCTGATTAAACA+AGG + chr5_1:8091785-8091804 None:intergenic 40.0%
TCTCAAAACGGGGAATTTGA+CGG + chr5_1:8091808-8091827 None:intergenic 40.0%
!!! GGATGATTGCTTTTTCGTAG+TGG + chr5_1:8091869-8091888 None:intergenic 40.0%
TTTCTATTCACACCTCACTC+TGG + chr5_1:8092062-8092081 None:intergenic 40.0%
GATGAAATTCAGCGAAGTAC+GGG + chr5_1:8092106-8092125 None:intergenic 40.0%
! GAGATTTCTTCTCAGGTTGT+AGG - chr5_1:8092133-8092152 Msa0715170:three_prime_UTR 40.0%
TGAAGTTGTAGCTTCATCCA+TGG + chr5_1:8092188-8092207 None:intergenic 40.0%
!!! TCGTCGGTGTTGAGAATTTT+CGG + chr5_1:8092249-8092268 None:intergenic 40.0%
TTCTTCATCGGAGATATCAC+CGG + chr5_1:8092305-8092324 None:intergenic 40.0%
!! CCTTTCTTCATCGAGTTGAA+CGG + chr5_1:8092359-8092378 None:intergenic 40.0%
!! TTTTTGGAGAAGAAATCGGG+CGG + chr5_1:8092936-8092955 None:intergenic 40.0%
TCTTCAAGCTCAAATTCCGT+TGG + chr5_1:8092964-8092983 None:intergenic 40.0%
!! TCGTTGGTTCGTTCTTTGAA+TGG - chr5_1:8093406-8093425 Msa0715170:five_prime_UTR 40.0%
!! CGTTGGTTCGTTCTTTGAAT+GGG - chr5_1:8093407-8093426 Msa0715170:five_prime_UTR 40.0%
GAAACGCGATTATTTGCAAG+TGG + chr5_1:8091584-8091603 None:intergenic 40.0%
TAACTAACGGGGGATGAAAA+CGG + chr5_1:8091746-8091765 None:intergenic 40.0%
TGACACGTCGTTGTAACTAA+CGG + chr5_1:8091759-8091778 None:intergenic 40.0%
CGCCGTTATCTGATTAAACA+AGG + chr5_1:8091785-8091804 None:intergenic 40.0%
TCTCAAAACGGGGAATTTGA+CGG + chr5_1:8091808-8091827 None:intergenic 40.0%
!!! GGATGATTGCTTTTTCGTAG+TGG + chr5_1:8091869-8091888 None:intergenic 40.0%
TTTCTATTCACACCTCACTC+TGG + chr5_1:8092062-8092081 None:intergenic 40.0%
GATGAAATTCAGCGAAGTAC+GGG + chr5_1:8092106-8092125 None:intergenic 40.0%
! GAGATTTCTTCTCAGGTTGT+AGG - chr5_1:8092133-8092152 Msa0715170:three_prime_UTR 40.0%
TGAAGTTGTAGCTTCATCCA+TGG + chr5_1:8092188-8092207 None:intergenic 40.0%
!!! TCGTCGGTGTTGAGAATTTT+CGG + chr5_1:8092249-8092268 None:intergenic 40.0%
TTCTTCATCGGAGATATCAC+CGG + chr5_1:8092305-8092324 None:intergenic 40.0%
!! CCTTTCTTCATCGAGTTGAA+CGG + chr5_1:8092359-8092378 None:intergenic 40.0%
!! TTTTTGGAGAAGAAATCGGG+CGG + chr5_1:8092936-8092955 None:intergenic 40.0%
TCTTCAAGCTCAAATTCCGT+TGG + chr5_1:8092964-8092983 None:intergenic 40.0%
!! TCGTTGGTTCGTTCTTTGAA+TGG - chr5_1:8093406-8093425 Msa0715170:five_prime_UTR 40.0%
!! CGTTGGTTCGTTCTTTGAAT+GGG - chr5_1:8093407-8093426 Msa0715170:five_prime_UTR 40.0%
ATAGGAATGGGAAGAGGATG+AGG + chr5_1:8091703-8091722 None:intergenic 45.0%
GTTGGAATAGGAATGGGAAG+AGG + chr5_1:8091709-8091728 None:intergenic 45.0%
! ACGGAGAAACGGTGTTTTGT+TGG + chr5_1:8091727-8091746 None:intergenic 45.0%
ACACGTCGTTGTAACTAACG+GGG + chr5_1:8091757-8091776 None:intergenic 45.0%
GACACGTCGTTGTAACTAAC+GGG + chr5_1:8091758-8091777 None:intergenic 45.0%
GATTCCCCCGTCACTATTAA+CGG - chr5_1:8091826-8091845 Msa0715170:three_prime_UTR 45.0%
AGTCTCCGTTAATAGTGACG+GGG + chr5_1:8091834-8091853 None:intergenic 45.0%
CAGTCTCCGTTAATAGTGAC+GGG + chr5_1:8091835-8091854 None:intergenic 45.0%
CCAGTCTCCGTTAATAGTGA+CGG + chr5_1:8091836-8091855 None:intergenic 45.0%
!! CTTTTTCGTAGTGGTGTGTG+TGG + chr5_1:8091860-8091879 None:intergenic 45.0%
ATTGCTTGAACAACAGGACG+CGG + chr5_1:8092332-8092351 None:intergenic 45.0%
GGATCCATTGCTTGAACAAC+AGG + chr5_1:8092338-8092357 None:intergenic 45.0%
CCGTTCAACTCGATGAAGAA+AGG - chr5_1:8092356-8092375 Msa0715170:CDS 45.0%
GTGAAGGATGTTCCATTGGA+AGG - chr5_1:8093130-8093149 Msa0715170:five_prime_UTR 45.0%
!! GGGATGTGGTGGGTTATTTA+TGG - chr5_1:8093361-8093380 Msa0715170:five_prime_UTR 45.0%
ATAGGAATGGGAAGAGGATG+AGG + chr5_1:8091703-8091722 None:intergenic 45.0%
GTTGGAATAGGAATGGGAAG+AGG + chr5_1:8091709-8091728 None:intergenic 45.0%
! ACGGAGAAACGGTGTTTTGT+TGG + chr5_1:8091727-8091746 None:intergenic 45.0%
ACACGTCGTTGTAACTAACG+GGG + chr5_1:8091757-8091776 None:intergenic 45.0%
GACACGTCGTTGTAACTAAC+GGG + chr5_1:8091758-8091777 None:intergenic 45.0%
GATTCCCCCGTCACTATTAA+CGG - chr5_1:8091826-8091845 Msa0715170:three_prime_UTR 45.0%
AGTCTCCGTTAATAGTGACG+GGG + chr5_1:8091834-8091853 None:intergenic 45.0%
CAGTCTCCGTTAATAGTGAC+GGG + chr5_1:8091835-8091854 None:intergenic 45.0%
CCAGTCTCCGTTAATAGTGA+CGG + chr5_1:8091836-8091855 None:intergenic 45.0%
!! CTTTTTCGTAGTGGTGTGTG+TGG + chr5_1:8091860-8091879 None:intergenic 45.0%
ATTGCTTGAACAACAGGACG+CGG + chr5_1:8092332-8092351 None:intergenic 45.0%
GGATCCATTGCTTGAACAAC+AGG + chr5_1:8092338-8092357 None:intergenic 45.0%
CCGTTCAACTCGATGAAGAA+AGG - chr5_1:8092356-8092375 Msa0715170:CDS 45.0%
GTGAAGGATGTTCCATTGGA+AGG - chr5_1:8093130-8093149 Msa0715170:five_prime_UTR 45.0%
!! GGGATGTGGTGGGTTATTTA+TGG - chr5_1:8093361-8093380 Msa0715170:five_prime_UTR 45.0%
ACTGCGTTTGCGTTTGCGTT+TGG + chr5_1:8091655-8091674 None:intergenic 50.0%
GGGGGATGAAAACGGAGAAA+CGG + chr5_1:8091738-8091757 None:intergenic 50.0%
CACGTCGTTGTAACTAACGG+GGG + chr5_1:8091756-8091775 None:intergenic 50.0%
TGACGGGGGAATCTCAAAAC+GGG + chr5_1:8091819-8091838 None:intergenic 50.0%
! GTGACGGGGGAATCTCAAAA+CGG + chr5_1:8091820-8091839 None:intergenic 50.0%
GTCTCCGTTAATAGTGACGG+GGG + chr5_1:8091833-8091852 None:intergenic 50.0%
CCGTCACTATTAACGGAGAC+TGG - chr5_1:8091833-8091852 Msa0715170:three_prime_UTR 50.0%
!! TTGATAACGCGCGTGGAGTA+CGG + chr5_1:8091937-8091956 None:intergenic 50.0%
ACACCTGAAAAGCCAGAGTG+AGG - chr5_1:8092047-8092066 Msa0715170:three_prime_UTR 50.0%
!!! ACACCTCACTCTGGCTTTTC+AGG + chr5_1:8092053-8092072 None:intergenic 50.0%
! CTTCTCAGGTTGTAGGTGTC+AGG - chr5_1:8092140-8092159 Msa0715170:three_prime_UTR 50.0%
TTCATCGGAGATATCACCGG+AGG + chr5_1:8092302-8092321 None:intergenic 50.0%
GGACGCGGGAAATTCTTCAT+CGG + chr5_1:8092317-8092336 None:intergenic 50.0%
TTGCTTGAACAACAGGACGC+GGG + chr5_1:8092331-8092350 None:intergenic 50.0%
GCGTCCTGTTGTTCAAGCAA+TGG - chr5_1:8092331-8092350 Msa0715170:exon 50.0%
ATTGGAAGGACGCTACGAGT+GGG - chr5_1:8093144-8093163 Msa0715170:five_prime_UTR 50.0%
ACTGCGTTTGCGTTTGCGTT+TGG + chr5_1:8091655-8091674 None:intergenic 50.0%
GGGGGATGAAAACGGAGAAA+CGG + chr5_1:8091738-8091757 None:intergenic 50.0%
CACGTCGTTGTAACTAACGG+GGG + chr5_1:8091756-8091775 None:intergenic 50.0%
TGACGGGGGAATCTCAAAAC+GGG + chr5_1:8091819-8091838 None:intergenic 50.0%
! GTGACGGGGGAATCTCAAAA+CGG + chr5_1:8091820-8091839 None:intergenic 50.0%
GTCTCCGTTAATAGTGACGG+GGG + chr5_1:8091833-8091852 None:intergenic 50.0%
CCGTCACTATTAACGGAGAC+TGG - chr5_1:8091833-8091852 Msa0715170:three_prime_UTR 50.0%
!! TTGATAACGCGCGTGGAGTA+CGG + chr5_1:8091937-8091956 None:intergenic 50.0%
ACACCTGAAAAGCCAGAGTG+AGG - chr5_1:8092047-8092066 Msa0715170:three_prime_UTR 50.0%
!!! ACACCTCACTCTGGCTTTTC+AGG + chr5_1:8092053-8092072 None:intergenic 50.0%
! CTTCTCAGGTTGTAGGTGTC+AGG - chr5_1:8092140-8092159 Msa0715170:three_prime_UTR 50.0%
TTCATCGGAGATATCACCGG+AGG + chr5_1:8092302-8092321 None:intergenic 50.0%
GGACGCGGGAAATTCTTCAT+CGG + chr5_1:8092317-8092336 None:intergenic 50.0%
TTGCTTGAACAACAGGACGC+GGG + chr5_1:8092331-8092350 None:intergenic 50.0%
GCGTCCTGTTGTTCAAGCAA+TGG - chr5_1:8092331-8092350 Msa0715170:exon 50.0%
ATTGGAAGGACGCTACGAGT+GGG - chr5_1:8093144-8093163 Msa0715170:five_prime_UTR 50.0%
GACGGGGGAATCTCAAAACG+GGG + chr5_1:8091818-8091837 None:intergenic 55.0%
!! GAGAGTGTTGATAACGCGCG+TGG + chr5_1:8091944-8091963 None:intergenic 55.0%
CGCTCAAGCTGCATTAGCCA+TGG - chr5_1:8092168-8092187 Msa0715170:three_prime_UTR 55.0%
ACCGACGATTCTTCCGCAAC+CGG - chr5_1:8092261-8092280 Msa0715170:three_prime_UTR 55.0%
ACCGGTTGCGGAAGAATCGT+CGG + chr5_1:8092265-8092284 None:intergenic 55.0%
CACTCGTAGCGTCCTTCCAA+TGG + chr5_1:8093145-8093164 None:intergenic 55.0%
CATTGGAAGGACGCTACGAG+TGG - chr5_1:8093143-8093162 Msa0715170:five_prime_UTR 55.0%
GACGGGGGAATCTCAAAACG+GGG + chr5_1:8091818-8091837 None:intergenic 55.0%
!! GAGAGTGTTGATAACGCGCG+TGG + chr5_1:8091944-8091963 None:intergenic 55.0%
CGCTCAAGCTGCATTAGCCA+TGG - chr5_1:8092168-8092187 Msa0715170:three_prime_UTR 55.0%
ACCGACGATTCTTCCGCAAC+CGG - chr5_1:8092261-8092280 Msa0715170:three_prime_UTR 55.0%
ACCGGTTGCGGAAGAATCGT+CGG + chr5_1:8092265-8092284 None:intergenic 55.0%
CACTCGTAGCGTCCTTCCAA+TGG + chr5_1:8093145-8093164 None:intergenic 55.0%
CATTGGAAGGACGCTACGAG+TGG - chr5_1:8093143-8093162 Msa0715170:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 8091523 8093496 8091523 ID=Msa0715170;Name=Msa0715170
chr5_1 mRNA 8091523 8093496 8091523 ID=Msa0715170-mRNA-1;Parent=Msa0715170;Name=Msa0715170-mRNA-1;_AED=0.04;_eAED=0.04;_QI=607|1|1|1|1|0.66|3|738|122
chr5_1 exon 8091523 8091905 8091523 ID=Msa0715170-mRNA-1:exon:3366;Parent=Msa0715170-mRNA-1
chr5_1 exon 8091988 8092441 8091988 ID=Msa0715170-mRNA-1:exon:3365;Parent=Msa0715170-mRNA-1
chr5_1 exon 8092620 8093496 8092620 ID=Msa0715170-mRNA-1:exon:3364;Parent=Msa0715170-mRNA-1
chr5_1 five_prime_UTR 8092890 8093496 8092890 ID=Msa0715170-mRNA-1:five_prime_utr;Parent=Msa0715170-mRNA-1
chr5_1 CDS 8092620 8092889 8092620 ID=Msa0715170-mRNA-1:cds;Parent=Msa0715170-mRNA-1
chr5_1 CDS 8092343 8092441 8092343 ID=Msa0715170-mRNA-1:cds;Parent=Msa0715170-mRNA-1
chr5_1 three_prime_UTR 8091988 8092342 8091988 ID=Msa0715170-mRNA-1:three_prime_utr;Parent=Msa0715170-mRNA-1
chr5_1 three_prime_UTR 8091523 8091905 8091523 ID=Msa0715170-mRNA-1:three_prime_utr;Parent=Msa0715170-mRNA-1
Gene Sequence

>Msa0715170

ATGGAGATTTCTTCTCAGGTTGTAGGTGTCAGGACTCGCGCTCAAGCTGCATTAGCCATGGATGAAGCTACAACTTCATCTCCAAAAACTTCAAAGAGAAGAAAAATCAACAACAACCGAAAATTCTCAACACCGACGATTCTTCCGCAACCGGTAAAAGATGAAAAATCCTCCGGTGATATCTCCGATGAAGAATTTCCCGCGTCCTGTTGTTCAAGCAATGGATCCGTTCAACTCGATGAAGAAAGGATCAAATCGTTAGATCTAGAGGTGGAGAGCGCGCAAGTTGAAACGTCGACGTGCAATTACAGTGAAGAAGAATTTCAGAGGTTTTTGAAATTTGAATTTGCTTTGGATTCTTTGAGTTAG
Protein sequence

>Msa0715170

MEISSQVVGVRTRAQAALAMDEATTSSPKTSKRRKINNNRKFSTPTILPQPVKDEKSSGDISDEEFPASCCSSNGSVQLDEERIKSLDLEVESAQVETSTCNYSEEEFQRFLKFEFALDSLS*