AlfalfaGEDB | Msa0735350

M. sativa cultivar zhongmu-4 / Msa0735350

KEGG: K07198|K12761
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0735350	XP_003616004.1	96.624	474	16	0	1	474	1	474	0.0	949

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0735350	sp\|Q9C562\|CIPKA_ARATH	64.744	468	143	5	1	461	1	453	0.0	630

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0735350	G7KFN1	96.624	474	16	0	1	474	1	474	0.0	949

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0735350	Msa0771880	0.907620	4.162649e-81	-8.615850e-47
Msa0735350	Msa0814370	0.805444	1.331093e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0735350	MtrunA17_Chr5g0432471	96.624	474	16	0	1	474	1	474	0.0	949
Msa0735350	MtrunA17_Chr3g0107011	69.011	455	110	2	7	455	9	438	0.0	640
Msa0735350	MtrunA17_Chr7g0244521	63.158	456	148	3	1	455	1	437	0.0	592
Msa0735350	MtrunA17_Chr4g0013131	61.538	468	152	6	1	467	1	441	0.0	582
Msa0735350	MtrunA17_Chr2g0284011	53.092	469	185	6	1	460	1	443	2.44e-177	504
Msa0735350	MtrunA17_Chr2g0284031	52.298	457	195	5	4	451	23	465	8.30e-170	488
Msa0735350	MtrunA17_Chr8g0379091	49.587	484	210	9	7	473	21	487	3.20e-160	463
Msa0735350	MtrunA17_Chr1g0150321	51.528	458	184	5	2	458	3	423	6.52e-156	449
Msa0735350	MtrunA17_Chr4g0001331	47.948	463	212	7	2	458	10	449	2.08e-150	436
Msa0735350	MtrunA17_Chr5g0441011	48.018	454	205	9	12	462	13	438	4.44e-150	435

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0735350	AT5G58380.1	64.744	468	143	5	1	461	1	453	0.0	630
Msa0735350	AT5G07070.1	63.425	473	153	1	1	473	1	453	0.0	593
Msa0735350	AT5G45820.1	56.209	459	165	5	1	457	1	425	0.0	521
Msa0735350	AT5G01810.1	53.696	460	170	4	1	456	1	421	2.35e-173	494
Msa0735350	AT5G01810.2	53.696	460	170	4	1	456	1	421	2.35e-173	494
Msa0735350	AT4G18700.1	52.349	447	199	6	7	451	21	455	1.51e-164	474
Msa0735350	AT4G30960.1	53.201	453	179	5	3	453	15	436	4.99e-164	471
Msa0735350	AT5G45810.1	51.656	453	204	7	3	451	19	460	7.40e-163	469
Msa0735350	AT5G25110.1	52.423	454	190	4	6	458	37	465	1.96e-162	468
Msa0735350	AT5G10930.1	51.087	460	199	5	1	458	1	436	4.09e-161	463

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Find 75 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAAAGAAAAGGGCTGC+TGG	0.522892	5_1:+50599904	Msa0735350:CDS
AAAATCAAATGGAGATACAT+TGG	0.502013	5_1:+50600029	Msa0735350:CDS
AAACTAGAGGGAGTCAATGA+AGG	0.552325	5_1:+50599934	Msa0735350:CDS
AAAGAAGACTATCTCTTCTC+TGG	0.453358	5_1:+50599588	Msa0735350:CDS
AAAGCGGATATTTGGTCTTG+TGG	0.496689	5_1:+50599322	Msa0735350:CDS
AAATATTGAATGATAATATT+AGG	0.341320	5_1:+50600061	Msa0735350:CDS
AATCTGAAGGTATCAGATTT+TGG	0.217907	5_1:+50599187	Msa0735350:CDS
ACAAGACCAAATATCCGCTT+TGG	0.468411	5_1:-50599320	None:intergenic
ACCAAGCTGATGTTATAAAA+AGG	0.209517	5_1:+50598903	Msa0735350:CDS
ACGTCAGATTGAGGGCGAGT+CGG	0.651709	5_1:+50599633	Msa0735350:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGGAAAAGTTTACTATGGA+AGG	+	chr5_1:50598816-50598835	Msa0735350:CDS	30.0%
ACCAAGCTGATGTTATAAAA+AGG	+	chr5_1:50598903-50598922	Msa0735350:CDS	30.0%
CCAAGCTGATGTTATAAAAA+GGG	+	chr5_1:50598904-50598923	Msa0735350:CDS	30.0%
GTTATAGAATATGCTAAAGG+CGG	+	chr5_1:50599010-50599029	Msa0735350:CDS	30.0%
ATCAAATTGGAGTCATTGAA+AGG	-	chr5_1:50599382-50599401	None:intergenic	30.0%
TTTCAATGACTCCAATTTGA+TGG	+	chr5_1:50599381-50599400	Msa0735350:CDS	30.0%
CCTATACATTTCCATCAAAT+TGG	-	chr5_1:50599395-50599414	None:intergenic	30.0%
CCAATTTGATGGAAATGTAT+AGG	+	chr5_1:50599392-50599411	Msa0735350:CDS	30.0%
GACAAATCATTTGGATCAAA+TGG	+	chr5_1:50599687-50599706	Msa0735350:CDS	30.0%
ACTTCAACCAAATGGAAAAA+TGG	-	chr5_1:50600006-50600025	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	50598749	50600173	50598749	ID=Msa0735350;Name=Msa0735350
chr5_1	mRNA	50598749	50600173	50598749	ID=Msa0735350-mRNA-1;Parent=Msa0735350;Name=Msa0735350-mRNA-1;_AED=0.19;_eAED=0.20;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|474
chr5_1	exon	50598749	50600173	50598749	ID=Msa0735350-mRNA-1:exon:14253;Parent=Msa0735350-mRNA-1
chr5_1	CDS	50598749	50600173	50598749	ID=Msa0735350-mRNA-1:cds;Parent=Msa0735350-mRNA-1

Gene Sequence

>Msa0735350

ATGGAGAATACATCAAAAGTCTTGATGCAAAGATATGAATTAGGAAGATTACTAGGCCAGGGAACTTTTGGAAAAGTTTACTATGGAAGGAGTACAATAACTAACCAAGGTGTAGCTATTAAAATGATTGACAAAGATAAGGTTATCAAAAATGACCAAGCTGATGTTATAAAAAGGGAAATATCTGTTATGAGACTTGTAAAACATCCAAATATTATACAGCTTTTTGAAGTCATGGCTACAAGGAGTAAAATTTACTTTGTTATAGAATATGCTAAAGGCGGTGAGCTTTTCAAACAAGTATCAAAAGGAAAGCTGAAAGATGACGTTGCGCACAAATATTTCAAGCAGTTAATTAGTGCAGTTGATTTTTGTCACAGTAGAGGTGTATACCATAGAGACATAAAGCCAGAGAACATTTTATTAGATGAGAATGAGAATCTGAAGGTATCAGATTTTGGTTTAAGTGCTTTTACTGAATCCAAAAGGCAAGATGGTTTGTTGCATACAACTTGTGGAACTCCTGCTTATGTTGCTCCTGAAGTGATCAAAAGGAAGGGTTATGATGGTTCCAAAGCGGATATTTGGTCTTGTGGAGTTGTTCTTTTTGTTTTTCTTGCTGGTTATGTACCTTTCAATGACTCCAATTTGATGGAAATGTATAGGAAGATAAGTAAAGCAGAGTTTAAGTGTCCTAATTGGTTTCCGAAAAATGTTAAGAAGCTGCTGTGTAAGATGTTGGATCCAAATCCTGACACTAGGATTTCTATCGATAAGATTAAACATTGTTCTTGGTTTAAGCATGGATCAAACGGTAGACCGAAAAAACGTGAAGAGGAAAAGAAGACTATCTCTTCTCTGGCTACAAACTCTGCAGCCCCGACACGTCAGATTGAGGGCGAGTCGGTGTCTGACACTGACACCAAACTGACCATTGTGACAAATCATTTGGATCAAATGGATGAAACCGAAAAGCAAGAGTCGGTTTTGCCTTTGAGTATTAATGCATTTGATATTATCTCTCTTTCGTCTGGTTTTAACCTATGTGGATTTTTTGAAGATAGTTTTCAGAAAAAGGAAGCGAGGTTTACTTCAAAACAGCCTGCATCAACCATTATTTCAAGGCTGGAAGAGATTGCTAAGAGACTGAAGATGAAAATAAAGAAAAGGGCTGCTGGTTTGTTGAAACTAGAGGGAGTCAATGAAGGTAGGAAAGGGATTTTGTCTGTTGATGCTGAGATTTTTGAGGCTTCTCCATTTTTCCATTTGGTTGAAGTGAAAAAATCAAATGGAGATACATTGGAATATCAGAAAATATTGAATGATAATATTAGGCCTGCTCTTCAAGACATTGTTTGGGTTTGGCAAGGTGAAGTACAAGAACAACCACAACAGTTAGAGCCTCAGCAACAAGCACAGCCATAA

Protein sequence

>Msa0735350

MENTSKVLMQRYELGRLLGQGTFGKVYYGRSTITNQGVAIKMIDKDKVIKNDQADVIKREISVMRLVKHPNIIQLFEVMATRSKIYFVIEYAKGGELFKQVSKGKLKDDVAHKYFKQLISAVDFCHSRGVYHRDIKPENILLDENENLKVSDFGLSAFTESKRQDGLLHTTCGTPAYVAPEVIKRKGYDGSKADIWSCGVVLFVFLAGYVPFNDSNLMEMYRKISKAEFKCPNWFPKNVKKLLCKMLDPNPDTRISIDKIKHCSWFKHGSNGRPKKREEEKKTISSLATNSAAPTRQIEGESVSDTDTKLTIVTNHLDQMDETEKQESVLPLSINAFDIISLSSGFNLCGFFEDSFQKKEARFTSKQPASTIISRLEEIAKRLKMKIKKRAAGLLKLEGVNEGRKGILSVDAEIFEASPFFHLVEVKKSNGDTLEYQKILNDNIRPALQDIVWVWQGEVQEQPQQLEPQQQAQP*