AlfalfaGEDB | Msa0740300

M. sativa cultivar zhongmu-4 / Msa0740300

KEGG: -
GO: -
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740300	XP_003617085.2	96.784	995	32	0	18	1012	1	995	0.0	1949

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740300	sp\|Q0WR59\|Y5020_ARATH	41.779	1034	528	18	37	1009	26	1046	0.0	729

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740300	G7K2X9	96.784	995	32	0	18	1012	1	995	0.0	1949

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0740300	Msa0781460	0.808671	2.762158e-50	-8.615850e-47
Msa0740300	Msa0820590	0.815074	1.113642e-51	-8.615850e-47
Msa0740300	Msa0861000	0.812355	4.421216e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740300	MtrunA17_Chr5g0440581	96.784	995	32	0	18	1012	1	995	0.0	1949
Msa0740300	MtrunA17_Chr2g0292521	41.565	1061	516	18	35	1009	24	1066	0.0	744
Msa0740300	MtrunA17_Chr3g0129081	41.075	1042	510	18	37	1009	24	1030	0.0	677
Msa0740300	MtrunA17_Chr5g0437741	32.043	749	388	22	282	1010	120	767	2.59e-79	275
Msa0740300	MtrunA17_Chr5g0437741	31.650	297	169	12	51	343	88	354	2.38e-18	90.5
Msa0740300	MtrunA17_Chr5g0437741	33.333	222	127	7	254	460	139	354	4.29e-16	83.6
Msa0740300	MtrunA17_Chr5g0437741	31.795	195	117	2	360	547	105	290	2.44e-15	80.9
Msa0740300	MtrunA17_Chr1g0203631	28.571	987	533	32	103	1010	376	1269	6.02e-76	273
Msa0740300	MtrunA17_Chr1g0203631	31.311	511	273	16	40	542	31	471	1.10e-37	153
Msa0740300	MtrunA17_Chr1g0203631	32.867	429	231	14	80	461	449	867	1.93e-29	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0740300	AT2G27060.1	52.930	1024	439	9	18	1009	1	1013	0.0	984
Msa0740300	AT2G27060.3	53.061	931	397	8	108	1009	1	920	0.0	896
Msa0740300	AT5G10020.1	41.779	1034	528	18	37	1009	26	1046	0.0	729
Msa0740300	AT2G27060.2	50.963	779	339	9	18	764	1	768	0.0	696
Msa0740300	AT4G20940.1	40.210	1047	537	15	36	1009	7	1037	0.0	681
Msa0740300	AT5G10020.2	39.024	1025	521	23	37	1009	26	998	0.0	640
Msa0740300	AT5G07280.1	28.864	977	525	36	86	1009	333	1192	1.64e-70	256
Msa0740300	AT5G07280.1	29.895	475	268	11	125	547	84	545	2.21e-34	143
Msa0740300	AT1G74360.1	27.812	960	534	39	109	1009	229	1088	9.32e-65	238
Msa0740300	AT1G74360.1	30.482	456	247	15	65	490	68	483	2.43e-36	149

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Find 207 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTTCCAATGAGTTAT+TGG	0.208847	5_1:+59189703	None:intergenic
AAAAGTTGAGAAGTTTCGTT+TGG	0.407569	5_1:+59189749	None:intergenic
AAATCGGGAGAAGTCGTTTC+AGG	0.270120	5_1:-59187701	Msa0740300:CDS
AACCAGGATTCTCGAACTGA+TGG	0.560063	5_1:+59188841	None:intergenic
AAGAGTGATGTCTACGCGTT+CGG	0.478589	5_1:-59187755	Msa0740300:CDS
AAGATTAGTCAATTTGGTCG+AGG	0.534499	5_1:+59189589	None:intergenic
AAGCTTCCTAAGAGTCATTG+AGG	0.549478	5_1:+59189940	None:intergenic
AAGTCTCCCACCAGTTTATC+CGG	0.464431	5_1:+59188801	None:intergenic
AATATCAGCATAGTATTTCC+TGG	0.417376	5_1:+59189257	None:intergenic
AATATTCCATACCAGTTCTG+AGG	0.638751	5_1:+59191067	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAATGCTGGTGTTTAGATT+AGG	-	chr5_1:59187332-59187351	Msa0740300:three_prime_UTR	30.0%
TATGGAATATTGTGTAGTGA+AGG	-	chr5_1:59187542-59187561	Msa0740300:CDS	30.0%
CAATGTTATATCCATCACTT+TGG	-	chr5_1:59187565-59187584	Msa0740300:CDS	30.0%
TTGTCTCTCAACAAATTCAA+CGG	-	chr5_1:59187728-59187747	Msa0740300:CDS	30.0%
TCAAGTTTGTGAAACACATT+TGG	+	chr5_1:59187833-59187852	None:intergenic	30.0%
GAATGTTAGTCATAATTCCT+TGG	-	chr5_1:59188021-59188040	Msa0740300:CDS	30.0%
GTTGTTACTCAAATCTATGA+TGG	+	chr5_1:59188738-59188757	None:intergenic	30.0%
ATTTGTCAAGGATCAAGTAT+TGG	-	chr5_1:59188771-59188790	Msa0740300:CDS	30.0%
TTTGTCAAGGATCAAGTATT+GGG	-	chr5_1:59188772-59188791	Msa0740300:CDS	30.0%
TTAAGTACAAACTCATTGTC+CGG	-	chr5_1:59188818-59188837	Msa0740300:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	59187320	59191299	59187320	ID=Msa0740300;Name=Msa0740300
chr5_1	mRNA	59187320	59191299	59187320	ID=Msa0740300-mRNA-1;Parent=Msa0740300;Name=Msa0740300-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|1\|0.66\|1\|1\|1\|3\|155\|1012
chr5_1	exon	59187320	59188067	59187320	ID=Msa0740300-mRNA-1:exon:17038;Parent=Msa0740300-mRNA-1
chr5_1	exon	59188487	59189980	59188487	ID=Msa0740300-mRNA-1:exon:17037;Parent=Msa0740300-mRNA-1
chr5_1	exon	59190348	59191299	59190348	ID=Msa0740300-mRNA-1:exon:17036;Parent=Msa0740300-mRNA-1
chr5_1	CDS	59190348	59191299	59190348	ID=Msa0740300-mRNA-1:cds;Parent=Msa0740300-mRNA-1
chr5_1	CDS	59188487	59189980	59188487	ID=Msa0740300-mRNA-1:cds;Parent=Msa0740300-mRNA-1
chr5_1	CDS	59187475	59188067	59187475	ID=Msa0740300-mRNA-1:cds;Parent=Msa0740300-mRNA-1
chr5_1	three_prime_UTR	59187320	59187474	59187320	ID=Msa0740300-mRNA-1:three_prime_utr;Parent=Msa0740300-mRNA-1

Gene Sequence

>Msa0740300

ATGACTGTGTTGTTAATGCTGGTGTTTAGATTAGGAGCAGATAGGAAAGGAATGCAGGCTATCTGGTTGATGCTGTTCTTGTTGGTGAATACTACATTTGGGAATAGAGACATTGATGCACTTCTTGAACTCAAGAAGGGTATTCAAAATGACCCTTTTGGATTAGTTCTTAATTCTTGGGATTCTAAGTCATTAGAATCTAATGGATGTCCTCAGAACTGGTATGGAATATTGTGTAGTGAAGGCAATGTTATATCCATCACTTTGGACAATGCTAGTTTGGTTGGAGAGTTCAATTTCCTTGCAATTAGTAACCTTCCAATGCTTCACAACTTGTCAGTTGTAAACAATCACTTCACAGGAAGTATGCTACATATTAGTCCAATGAAATCACTTAAATTTCTCGATTTGTCTCTCAACAAATTCAACGGCTCGTTACCTCCTAGCTTCGTTGAATTGCACAGTTTAGTGTATCTGAATCTTTCTTCAAATGAATTTAGTGGCACTGTTCCAAATGTGTTTCACAAACTTGACCAGTTGAAGTATTTAGATTTTCACAGTAATAGCTTTTCGGGCGATATTATGGAAATCTTTTATCAGATGGGAAGTGTGTTACATGTTGACTTAAGCAACAACAAGTTTTCTGGTGCATTGGATTTAGGACTTGGAGATGTCTCATTTCTATTCTCAATTCAACATTTGAATGTTAGTCATAATTCCTTGGTTGGTGAACTATTTGCTCATGATGGTATGCCATACCTTGACAATTTAGAGGTCTTTGATGCTAGTAATAATCAGCTAGTTGGTAATATACCTTCCTTCACGTTTGTCGTATCGCTAAGGATTCTTCGGCTCGCATGCAATCAGTTGACAGGCTCATTGCCAGAAACTTTGTTGAAGGAAAGTTCAATGATGTTGTCTGAACTTGATCTTGGTCAAAACAAGCTTGAAGGTTTCATTGGAAGCATTACCTCAATGACTCTTAGGAAGCTTAATTTATCTTCAAACAAATTATCAGGCCCATTACCTCTCAAAGTAAGTCATTGTGCCATCATAGATTTGAGTAACAACATGCTGTCAGGTAATTTGTCAAGGATCAAGTATTGGGGTAACTATGTGGAAGTTATTCAGTTAAGTACAAACTCATTGTCCGGAACACTTCCAAACGAAACTTCTCAACTTTTACGGTTAACTTCACTTAAGGTATCCAATAACTCATTGGAAGGTTTTCTGCCACCGGTATTAGGAACATATCCAGAACTAAAAGAGATTGATCTTAGTCTTAACCGTCTTTCGGGGTTTCTCCTACCAACCCTTTTTGCCTCGACCAAATTGACTAATCTTAATCTCTCTAACAATATGTTTTCTGGTCCAATTCCTTTTGAACTTCAACTTCCAAATAATCCATTGGTTTCTGCTGAAAATTTTAGTTTGATGTATCTTGATCTTTCGAACAACAGCTTGAGTGGAATTCTTTCTTCGAAAATTAACGAGCTTCATAATTTGGTATATCTCAATCTATGTAACAACAAATTGGAAGGTACTATTCCTAATGATCTTCCAGATGAATTAAGAGAACTCAATGTGTCCTTAAATAATTTTTCTGGGGTTGTACCTGATAACTTATCGCAGTTTCCTGAATCAGCATTTCATCCAGGAAATACTATGCTGATATTTCCTAATTCTCAATCATCACCTAAAGATAGTTCCAATTCCAACCTAGGTTCGAGGTCACATAAGAAAACTTTCACTAGGAGTGTCTTGATTACATGTATAGTTACTGGTGTTTTTGTGATAGCTATAATGGCTGCCATGATTTATTATAGGATTCATCAGAAAAAAGGAAGTACTTCTAAACAAGATACAACAACAAGTGACATCATCCAAGAGAGTACTTCTCCATCAAAGAGAAGAAATTTGGAGTCATTACCGCCTTCTCAAAGTGATGATATCGGAAACATTAATCCAACTGTACAGAAGCCTAAAGATCTGGAATTAGTTAAGAACGAGGAAGGAACGTCTTCTCCAATGTCTACTATATCAGCTTCGAATCCTTCACCTTCTACAAGCCATCAGTTCGAGAATCCTGGTTCGCTCGAAGTATCCTCTCCGGATAAACTGGTGGGAGACTTGCATCTATTTGATGGATCCTTGATGCTTACTGCGGAAGAACTTTCGTGTGCTCCGGCTGAAGTTGTTGGTAGGAGTTGTCATGGAACACTTTACAAAGCTACACTTGAATCTGGTCATGTATTGGCTGTTAAATGGTTAAGAGAAGGAATAACTAAAGGAAAAAAGGAGTTGGCTAGGGAAATTAAGAAACTTGGGACAATCAAGCATCCAAACTTGGTTTCTTTTCTTGGTTGCTACTTAGGGCCAAAGGAGCATGAGAGATTGATTATATCAAATTACATGAATGCACATTCTTTGGACATTTATCTCCACGAGGCTGATAAAAGAAATCTTCATCCTTTGTCTCTTGATGAAAGGCTCAGGGTGGCAGTAGAAGTGGCTCGATGTCTACTTTATCTACACGCCGAGAAAGCCATACCTCATGGTAATCTCAAATCCACAAACATTCTACTAGAAACTCCCAACAGAAATGTCTTGCTCACTGACTACAGCCTGCACCGAATACTTACTGCTGCTGGTACTTCAGAGCAAATTTTGAATGCCGGTGCACTTGGTTACCGACCTCCCGAATTCGCTAGATCAACCAAACCATGCCCTTCTCTGAAGAGTGATGTCTACGCGTTCGGAGTTGTCTTGTTAGAGCTCCTAACAGGAAGAAAATCGGGAGAAGTCGTTTCAGGGATTCCTGGCATGGCTGAACTTACCGATTGGGTGAGATTCTTAGCTGAACATGATCGCTCGAACCAATGCTTTGAAAATTCCCTTATGGACAATCACAATGGAGAAGATTCATTTAGGATTCTTGACGACATGCTAAAAGTGGCTCTTAGATGCACCCTTTCAGCATCTGAGAGGCCTGATATGAAAACAGTCTTTGATGATCTTTCAACAATAAAGGAGAGTTGA

Protein sequence

>Msa0740300

MTVLLMLVFRLGADRKGMQAIWLMLFLLVNTTFGNRDIDALLELKKGIQNDPFGLVLNSWDSKSLESNGCPQNWYGILCSEGNVISITLDNASLVGEFNFLAISNLPMLHNLSVVNNHFTGSMLHISPMKSLKFLDLSLNKFNGSLPPSFVELHSLVYLNLSSNEFSGTVPNVFHKLDQLKYLDFHSNSFSGDIMEIFYQMGSVLHVDLSNNKFSGALDLGLGDVSFLFSIQHLNVSHNSLVGELFAHDGMPYLDNLEVFDASNNQLVGNIPSFTFVVSLRILRLACNQLTGSLPETLLKESSMMLSELDLGQNKLEGFIGSITSMTLRKLNLSSNKLSGPLPLKVSHCAIIDLSNNMLSGNLSRIKYWGNYVEVIQLSTNSLSGTLPNETSQLLRLTSLKVSNNSLEGFLPPVLGTYPELKEIDLSLNRLSGFLLPTLFASTKLTNLNLSNNMFSGPIPFELQLPNNPLVSAENFSLMYLDLSNNSLSGILSSKINELHNLVYLNLCNNKLEGTIPNDLPDELRELNVSLNNFSGVVPDNLSQFPESAFHPGNTMLIFPNSQSSPKDSSNSNLGSRSHKKTFTRSVLITCIVTGVFVIAIMAAMIYYRIHQKKGSTSKQDTTTSDIIQESTSPSKRRNLESLPPSQSDDIGNINPTVQKPKDLELVKNEEGTSSPMSTISASNPSPSTSHQFENPGSLEVSSPDKLVGDLHLFDGSLMLTAEELSCAPAEVVGRSCHGTLYKATLESGHVLAVKWLREGITKGKKELAREIKKLGTIKHPNLVSFLGCYLGPKEHERLIISNYMNAHSLDIYLHEADKRNLHPLSLDERLRVAVEVARCLLYLHAEKAIPHGNLKSTNILLETPNRNVLLTDYSLHRILTAAGTSEQILNAGALGYRPPEFARSTKPCPSLKSDVYAFGVVLLELLTGRKSGEVVSGIPGMAELTDWVRFLAEHDRSNQCFENSLMDNHNGEDSFRILDDMLKVALRCTLSASERPDMKTVFDDLSTIKES*