Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0743670 | XP_024640932.1 | 93.506 | 77 | 5 | 0 | 1 | 77 | 1 | 77 | 5.20e-43 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0743670 | G7K4G3 | 90.789 | 76 | 7 | 0 | 2 | 77 | 1 | 76 | 4.80e-41 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0743670 | Msa0863790 | 0.887495 | 1.356963e-72 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0743670 | MtrunA17_Chr5g0445581 | 90.789 | 76 | 7 | 0 | 2 | 77 | 1 | 76 | 9.25e-45 | 138 |
| Msa0743670 | MtrunA17_Chr2g0290831 | 41.538 | 65 | 38 | 0 | 2 | 66 | 1 | 65 | 9.22e-11 | 52.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0743670 | AT4G05030.2 | 44.444 | 72 | 39 | 1 | 4 | 75 | 3 | 73 | 2.23e-11 | 54.3 |
| Msa0743670 | AT4G05030.1 | 44.000 | 75 | 41 | 1 | 1 | 75 | 33 | 106 | 3.89e-11 | 54.7 |
Find 19 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATAGAGTGGTGATTAA+AGG | 0.272964 | 5_1:+64151209 | Msa0743670:CDS |
| TATACATAGTTATTCATCAT+TGG | 0.378479 | 5_1:-64151309 | None:intergenic |
| AGGTGTTACAATGGTTACTT+TGG | 0.427464 | 5_1:+64151175 | Msa0743670:intron |
| ACCATCAGCAATGGTACATA+TGG | 0.447473 | 5_1:-64150752 | None:intergenic |
| CAAGGGTGACTGAACGTTTG+AGG | 0.485328 | 5_1:+64151249 | Msa0743670:CDS |
| TACAATGGTTACTTTGGAAA+GGG | 0.497733 | 5_1:+64151181 | Msa0743670:CDS |
| TTACAATGGTTACTTTGGAA+AGG | 0.504948 | 5_1:+64151180 | Msa0743670:CDS |
| AAGGGTGACTGAACGTTTGA+GGG | 0.506906 | 5_1:+64151250 | Msa0743670:CDS |
| ACTAACAAGTCTAGCATGCC+TGG | 0.525033 | 5_1:-64151282 | None:intergenic |
| AAAATGCAAGAAGAAGATAT+TGG | 0.540381 | 5_1:+64150729 | Msa0743670:CDS |
| ATGGTTACTTTGGAAAGGGA+AGG | 0.567959 | 5_1:+64151185 | Msa0743670:CDS |
| GAGAAGATGTTGATGCAGCA+AGG | 0.572875 | 5_1:+64151231 | Msa0743670:CDS |
| AAGGGAAGGAAAAGATAGAG+TGG | 0.595147 | 5_1:+64151199 | Msa0743670:CDS |
| AGAAGATGTTGATGCAGCAA+GGG | 0.596117 | 5_1:+64151232 | Msa0743670:CDS |
| GAATGATATAGGTGTTACAA+TGG | 0.641061 | 5_1:+64151166 | Msa0743670:intron |
| GCCATATGTACCATTGCTGA+TGG | 0.651621 | 5_1:+64150751 | Msa0743670:CDS |
| TCAACACATACCATCAGCAA+TGG | 0.657350 | 5_1:-64150761 | None:intergenic |
| GTTTGAGGGAGAAAGTGACC+AGG | 0.682645 | 5_1:+64151264 | Msa0743670:CDS |
| AATGGTGCAGCAAAAGATCG+TGG | 0.693758 | 5_1:+64150684 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATAACAAATATTACAT+TGG | + | chr5_1:64150882-64150901 | Msa0743670:intron | 10.0% |
| !!! | ATTTTTAACTTTTCAATATT+AGG | + | chr5_1:64151109-64151128 | Msa0743670:intron | 10.0% |
| !! | GAAAAAAGAAATATGTATTA+TGG | - | chr5_1:64151067-64151086 | None:intergenic | 15.0% |
| !! | AATGAAAAAATATGGTTAAG+AGG | + | chr5_1:64150951-64150970 | Msa0743670:intron | 20.0% |
| ! | AAAATGCAAGAAGAAGATAT+TGG | + | chr5_1:64150729-64150748 | Msa0743670:CDS | 25.0% |
| ! | TAACAAATATTACATTGGTC+TGG | + | chr5_1:64150887-64150906 | Msa0743670:intron | 25.0% |
| ! | ACCTTGTGAATGAAAAAATA+TGG | + | chr5_1:64150943-64150962 | Msa0743670:intron | 25.0% |
| !!! | ACCATATTTTTTCATTCACA+AGG | - | chr5_1:64150947-64150966 | None:intergenic | 25.0% |
| !! | AACTTTTCAATATTAGGAAC+TGG | + | chr5_1:64151115-64151134 | Msa0743670:intron | 25.0% |
| ! | AATATTAGGAACTGGTAATA+CGG | + | chr5_1:64151123-64151142 | Msa0743670:intron | 25.0% |
| ! | TAATCGTTTGTGAATGATAT+AGG | + | chr5_1:64151155-64151174 | Msa0743670:intron | 25.0% |
| ! | GAATGATATAGGTGTTACAA+TGG | + | chr5_1:64151166-64151185 | Msa0743670:intron | 30.0% |
| ! | TTACAATGGTTACTTTGGAA+AGG | + | chr5_1:64151180-64151199 | Msa0743670:CDS | 30.0% |
| ! | TACAATGGTTACTTTGGAAA+GGG | + | chr5_1:64151181-64151200 | Msa0743670:CDS | 30.0% |
| AAAGATAGAGTGGTGATTAA+AGG | + | chr5_1:64151209-64151228 | Msa0743670:CDS | 30.0% | |
| GCAACTTGATTGATCTTTAG+TGG | - | chr5_1:64150984-64151003 | None:intergenic | 35.0% | |
| ! | AGGTGTTACAATGGTTACTT+TGG | + | chr5_1:64151175-64151194 | Msa0743670:intron | 35.0% |
| ! | TCTTCTTGCATTTTGCACAG+TGG | - | chr5_1:64150723-64150742 | None:intergenic | 40.0% |
| ACCATCAGCAATGGTACATA+TGG | - | chr5_1:64150755-64150774 | None:intergenic | 40.0% | |
| TCAACACATACCATCAGCAA+TGG | - | chr5_1:64150764-64150783 | None:intergenic | 40.0% | |
| GAGGATGACTAATCTTCATC+AGG | - | chr5_1:64151006-64151025 | None:intergenic | 40.0% | |
| ! | ATGGTTACTTTGGAAAGGGA+AGG | + | chr5_1:64151185-64151204 | Msa0743670:CDS | 40.0% |
| AAGGGAAGGAAAAGATAGAG+TGG | + | chr5_1:64151199-64151218 | Msa0743670:CDS | 40.0% | |
| AGAAGATGTTGATGCAGCAA+GGG | + | chr5_1:64151232-64151251 | Msa0743670:CDS | 40.0% | |
| ! | TTGCATTTTGCACAGTGGAG+GGG | - | chr5_1:64150718-64150737 | None:intergenic | 45.0% |
| ! | CTTGCATTTTGCACAGTGGA+GGG | - | chr5_1:64150719-64150738 | None:intergenic | 45.0% |
| ! | TCTTGCATTTTGCACAGTGG+AGG | - | chr5_1:64150720-64150739 | None:intergenic | 45.0% |
| GCCATATGTACCATTGCTGA+TGG | + | chr5_1:64150751-64150770 | Msa0743670:CDS | 45.0% | |
| GGACTCGAATCGCTTAAACA+AGG | + | chr5_1:64150908-64150927 | Msa0743670:intron | 45.0% | |
| TGAGCATCTTCGATTTCTCG+AGG | - | chr5_1:64151025-64151044 | None:intergenic | 45.0% | |
| GAGAAGATGTTGATGCAGCA+AGG | + | chr5_1:64151231-64151250 | Msa0743670:CDS | 45.0% | |
| AAGGGTGACTGAACGTTTGA+GGG | + | chr5_1:64151250-64151269 | Msa0743670:CDS | 45.0% | |
| ACTAACAAGTCTAGCATGCC+TGG | - | chr5_1:64151285-64151304 | None:intergenic | 45.0% | |
| CAAGGGTGACTGAACGTTTG+AGG | + | chr5_1:64151249-64151268 | Msa0743670:CDS | 50.0% | |
| GTTTGAGGGAGAAAGTGACC+AGG | + | chr5_1:64151264-64151283 | Msa0743670:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 64150685 | 64151322 | 64150685 | ID=Msa0743670;Name=Msa0743670 |
| chr5_1 | mRNA | 64150685 | 64151322 | 64150685 | ID=Msa0743670-mRNA-1;Parent=Msa0743670;Name=Msa0743670-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|77 |
| chr5_1 | exon | 64150685 | 64150772 | 64150685 | ID=Msa0743670-mRNA-1:exon:18943;Parent=Msa0743670-mRNA-1 |
| chr5_1 | exon | 64151177 | 64151322 | 64151177 | ID=Msa0743670-mRNA-1:exon:18944;Parent=Msa0743670-mRNA-1 |
| chr5_1 | CDS | 64150685 | 64150772 | 64150685 | ID=Msa0743670-mRNA-1:cds;Parent=Msa0743670-mRNA-1 |
| chr5_1 | CDS | 64151177 | 64151322 | 64151177 | ID=Msa0743670-mRNA-1:cds;Parent=Msa0743670-mRNA-1 |
| Gene Sequence |
| Protein sequence |
