AlfalfaGEDB | Msa0751610

M. sativa cultivar zhongmu-4 / Msa0751610

KEGG: -
GO: -
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751610	AES93667.1	86.839	775	46	4	12	743	22	783	0.0	1358

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751610	sp\|A0A075F7E9\|LERK1_ORYSI	36.469	776	413	17	23	735	35	793	2.51e-152	466

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751610	G7K2D3	86.839	775	46	4	12	743	22	783	0.0	1358

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0731180	Msa0751610	0.801603	8.329017e-49	-8.615850e-47
Msa0751610	Msa1132290	0.839490	1.535570e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751610	MtrunA17_Chr5g0394381	85.548	775	47	4	12	743	22	774	0.0	1335
Msa0751610	MtrunA17_Chr2g0311521	37.630	768	407	18	19	735	266	1012	5.35e-159	487
Msa0751610	MtrunA17_Chr1g0205561	45.283	530	234	9	222	743	2	483	5.62e-152	452
Msa0751610	MtrunA17_Chr2g0311541	36.198	768	417	18	19	735	24	769	6.02e-152	461
Msa0751610	MtrunA17_Chr2g0311551	36.446	771	417	18	14	735	260	1006	4.83e-148	458
Msa0751610	MtrunA17_Chr8g0338471	37.500	776	400	22	24	735	40	794	2.41e-143	440
Msa0751610	MtrunA17_Chr5g0399591	34.816	787	414	19	18	737	29	783	5.95e-141	433
Msa0751610	MtrunA17_Chr8g0338501	36.776	794	412	25	10	735	54	825	6.94e-139	429
Msa0751610	MtrunA17_Chr5g0399561	34.143	782	422	21	19	735	31	784	7.72e-129	402
Msa0751610	MtrunA17_Chr4g0063701	33.812	766	430	16	26	735	51	795	1.22e-127	399

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0751610	AT4G00340.1	30.248	767	460	22	16	735	21	759	2.33e-93	309
Msa0751610	AT4G00340.2	30.248	767	439	24	16	735	21	738	3.40e-93	308
Msa0751610	AT5G24080.2	29.052	802	467	28	10	735	26	801	5.20e-89	298
Msa0751610	AT1G34300.1	30.435	782	415	27	22	735	36	756	1.65e-88	296
Msa0751610	AT2G19130.1	30.065	775	453	24	16	735	26	766	1.04e-82	280
Msa0751610	AT5G24080.3	28.963	694	404	22	111	735	1	674	5.47e-79	268
Msa0751610	AT5G24080.1	28.963	694	404	22	111	735	1	674	5.47e-79	268
Msa0751610	AT4G21380.1	28.931	795	476	25	14	740	32	805	6.14e-78	268
Msa0751610	AT1G65790.1	28.820	805	460	28	14	740	30	799	1.23e-73	256
Msa0751610	AT5G35370.1	28.834	763	452	28	45	735	60	803	4.15e-71	249

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Find 118 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGAACTGATTGGGAA+AGG	0.532783	5_2:+5070297	Msa0751610:CDS
AAACTTGACAACGCTCCGTT+TGG	0.433889	5_2:-5071223	None:intergenic
AAATGGAACTGATTGGGAAA+GGG	0.456142	5_2:+5070298	Msa0751610:CDS
AAATGGCTTTGGTTGGATTA+TGG	0.298813	5_2:+5071746	Msa0751610:CDS
AACTCTCCCAAACAGTTCTT+AGG	0.290596	5_2:-5069909	None:intergenic
AAGCAACAGATGGATTCAGT+AGG	0.630342	5_2:+5070975	Msa0751610:CDS
AAGGAATTTGGTTCAACTGA+GGG	0.548384	5_2:-5069739	None:intergenic
AAGGTTTGAGCAAATGGCTT+TGG	0.398440	5_2:+5071735	Msa0751610:CDS
AAGTGCTCAATGATTTCAAA+AGG	0.466918	5_2:+5071716	Msa0751610:CDS
AATAATGAGTCCAACACATT+TGG	0.390398	5_2:+5070749	Msa0751610:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTAACCATACATACGTATA+CGG	+	chr5_2:5069312-5069331	Msa0751610:intron	30.0%
AATTTCATGCTTGAAATGCA+GGG	+	chr5_2:5070030-5070049	Msa0751610:CDS	30.0%
ATCAAGAAGATGTGTCTAAA+GGG	+	chr5_2:5070441-5070460	Msa0751610:CDS	30.0%
TCATTAATGAGAGCAAAATC+AGG	-	chr5_2:5070560-5070579	None:intergenic	30.0%
AATAATGAGTCCAACACATT+TGG	+	chr5_2:5070749-5070768	Msa0751610:CDS	30.0%
TATCTAAATGCAACAGCTAT+TGG	+	chr5_2:5070914-5070933	Msa0751610:CDS	30.0%
ATGACTGAGCTCAAAATTAT+AGG	+	chr5_2:5071118-5071137	Msa0751610:CDS	30.0%
TTGATCTTTGTTCAAAAGCT+TGG	-	chr5_2:5071431-5071450	None:intergenic	30.0%
AAGTGCTCAATGATTTCAAA+AGG	+	chr5_2:5071716-5071735	Msa0751610:CDS	30.0%
TTTCAAAAGGTTTGAGCAAA+TGG	+	chr5_2:5071729-5071748	Msa0751610:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	5068665	5071852	5068665	ID=Msa0751610;Name=Msa0751610
chr5_2	mRNA	5068665	5071852	5068665	ID=Msa0751610-mRNA-1;Parent=Msa0751610;Name=Msa0751610-mRNA-1;_AED=0.12;_eAED=0.14;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|743
chr5_2	exon	5068665	5068707	5068665	ID=Msa0751610-mRNA-1:exon:1989;Parent=Msa0751610-mRNA-1
chr5_2	exon	5069584	5070074	5069584	ID=Msa0751610-mRNA-1:exon:1990;Parent=Msa0751610-mRNA-1
chr5_2	exon	5070155	5071852	5070155	ID=Msa0751610-mRNA-1:exon:1991;Parent=Msa0751610-mRNA-1
chr5_2	CDS	5068665	5068707	5068665	ID=Msa0751610-mRNA-1:cds;Parent=Msa0751610-mRNA-1
chr5_2	CDS	5069584	5070074	5069584	ID=Msa0751610-mRNA-1:cds;Parent=Msa0751610-mRNA-1
chr5_2	CDS	5070155	5071852	5070155	ID=Msa0751610-mRNA-1:cds;Parent=Msa0751610-mRNA-1

Gene Sequence

>Msa0751610

ATGTCCTTGTGGGTCCGCCGCTTTCCTACAAAATATTTCCTAGACTTAAAATCAAGCATCACAGCCGGATCGAATTCAACATGGAAATCACCTTCTGGCGATTTTGAATTCGGATTCTACCCTCTTCTCAATGGTCTTTTTCTACCAGGAATATGGTTTGCAAAAATCCCACAAAAAACATTGGTATGGTACCAAAGTCCCTCAGTTGAACCAAATTCCTTACTCCAACTCACATCTGAAGGACACCTTGTGATAACACGCCCTAATGGAACCACTTCTCATACCATAGACAACATTGGAGGAGATAGTGAAGCTGCAAATTCTGCTCATATGCAAGATGATGGAAATTTCGTCTTGAAAGACTCCAACCTAAGAACTGTTTGGGAGAGTTTTAATTCTCCAGCTAACACAATCGTACCTGGACAAACCTTGAAAAGCAATCAAATTCTTTATTCTAAAGGGAAAGGAGATTCAAACTACTCAATGGGGAATTTCATGCTTGAAATGCAGGGTGATGGTAACTTGATCCTCAAGTTCTCTATTGTATCTTGCAACTGGAACTGGAAACATTATTTACTCATTAGAGTAAAAAGTACTCCAACTCCTGTAAAAGATTACTATCACCGTGCTACAATTGATGAAAATGGAAATTTCCAGCAATACGTGTATCATAAAGAAAATGGAACTGATTGGGAAAGGGTTTGGAGAGCTATAGATGATCCATGTAGAGTGGATTATGTTTGTGGAATTTATGGTTTATGCACTTCTCCTGATAATGAATCAGTGAATTGTGAGTGTATTCAAGGTCATATTCCATTCGATCAAGAAGATGTGTCTAAAGGGTGTCGTCCAAAAACTGTAATCAATTATTGTAGTGGATCTTCAATGATGAATTTTGAGCTGCGTGTGTTTGATGACACTGATTTTCAATTTTATCCTGATTTTGCTCTCATTAATGATGTTGATTTGGAAAGTTGTAAGAAATCTGTGATAGATGATTGCAATATCATTGCTGCTACTTATAATTCTTCAACTTCAACATGTGCTAAGAAGAGAATGCCGTTGCTGAATGCTAGAAACAGTTCATCTTCCAAGGGACAAAAAGCACTTCTCAAAGTTCCTTATAGTAATAATGAGTCCAACACATTTGGAGTTTCAAAGAACAAGAGTTTTAATGTGAGAGTTTTTTTGAAGGTTATGGTTGCAATTAGTGCAACTCTTGCATGCTTCTTTGGTGCTCTTGCTGCTTATTATCATCCTTTTGTAAAGAGACTAATAACAAGAAGAAAGAAGTATCTAAATGCAACAGCTATTGGAATCAACTTTAGAGAATTCACATTCCAAGAGCTGCATGAAGCAACAGATGGATTCAGTAGGATTCTTGGCAAGGGATCTTCAGGGAAAGTCTACCGCGGTACTTTGATTGTAGATGACGTGGAAATTGGTATTGCAGTGAAGAAACTTGAAAAGAAGATTGAGAAAAGTGAGAATGAGTTCATGACTGAGCTCAAAATTATAGGTCTTACACATCACAAAAATTTGGTGAAACTTTTGGGATTTTGCATGGAGAATAATCATAGACTACTTGTTTATGAGCTAATGCCAAACGGAGCGTTGTCAAGTTTATTGTTTGGAAAAGGAGAGAGACCTCAATGGAGTCAAAGGGTTGAAATGGCTCTTGGAATAGCAAAAGGCTTACTCTACTTGCATGAAGAGTGTGAAACACAGATCATACATTGTGATATCAAGCCACAAAATGTGTTACTTGATGCTTATCATATTGCAAAGATTGCAGATTTTGGACTTTCCAAGCTTTTGAACAAAGATCAAACTAGAACAAGTACTAATTTTAGAGGGACAATAGGGTACATAGCACCTGAATGGTTAAGGAGTGCACCAATAACTGCAAAAGTTGATGTTTTCAGCTATGGAGTTGTGCTACTTGAGATTATTTGTTGTAGGAGGCATGTTGAGTCATATCAGAGTCATGATGATGATGAAAGAGGAAGTGAAGATGATGATTTGGTTCTTGTTAATTTAGTTTTAAGGTGCATGGTTACTAGGAAGCTAGAGATAGTGGTAAGTGATGACTTAGAAGTGCTCAATGATTTCAAAAGGTTTGAGCAAATGGCTTTGGTTGGATTATGGTGTCTTCATCCAAACCCAACTCTTGGACCTTCAATGAAGAAAGTTACTCAAATGATGGCAAATCAGAACTCTTATAGTTTGTGA

Protein sequence

>Msa0751610

MSLWVRRFPTKYFLDLKSSITAGSNSTWKSPSGDFEFGFYPLLNGLFLPGIWFAKIPQKTLVWYQSPSVEPNSLLQLTSEGHLVITRPNGTTSHTIDNIGGDSEAANSAHMQDDGNFVLKDSNLRTVWESFNSPANTIVPGQTLKSNQILYSKGKGDSNYSMGNFMLEMQGDGNLILKFSIVSCNWNWKHYLLIRVKSTPTPVKDYYHRATIDENGNFQQYVYHKENGTDWERVWRAIDDPCRVDYVCGIYGLCTSPDNESVNCECIQGHIPFDQEDVSKGCRPKTVINYCSGSSMMNFELRVFDDTDFQFYPDFALINDVDLESCKKSVIDDCNIIAATYNSSTSTCAKKRMPLLNARNSSSSKGQKALLKVPYSNNESNTFGVSKNKSFNVRVFLKVMVAISATLACFFGALAAYYHPFVKRLITRRKKYLNATAIGINFREFTFQELHEATDGFSRILGKGSSGKVYRGTLIVDDVEIGIAVKKLEKKIEKSENEFMTELKIIGLTHHKNLVKLLGFCMENNHRLLVYELMPNGALSSLLFGKGERPQWSQRVEMALGIAKGLLYLHEECETQIIHCDIKPQNVLLDAYHIAKIADFGLSKLLNKDQTRTSTNFRGTIGYIAPEWLRSAPITAKVDVFSYGVVLLEIICCRRHVESYQSHDDDERGSEDDDLVLVNLVLRCMVTRKLEIVVSDDLEVLNDFKRFEQMALVGLWCLHPNPTLGPSMKKVTQMMANQNSYSL*