AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0765350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0765350 MtrunA17_Chr5g0416431 98.925 93 1 0 1 93 1 93 7.68e-61 180
Msa0765350 MtrunA17_Chr8g0362131 79.762 84 16 1 10 93 10 92 1.71e-42 134
Msa0765350 MtrunA17_Chr7g0217141 72.826 92 24 1 1 92 1 91 8.00e-41 129
Msa0765350 MtrunA17_Chr6g0453041 69.737 76 23 0 17 92 15 90 3.68e-34 112
Msa0765350 MtrunA17_Chr3g0093001 74.026 77 18 1 17 93 17 91 3.81e-34 112
Msa0765350 MtrunA17_Chr7g0237771 45.946 74 40 0 8 81 3 76 9.86e-12 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0765350 AT1G26945.1 83.784 74 12 0 19 92 19 92 3.26e-39 125
Msa0765350 AT3G28857.1 64.894 94 30 2 1 93 1 92 3.87e-36 118
Msa0765350 AT3G47710.1 69.149 94 25 3 1 93 1 91 3.62e-35 115
Msa0765350 AT5G15160.1 73.333 75 19 1 19 92 18 92 3.39e-32 108
Msa0765350 AT1G74500.1 72.727 77 18 2 17 92 17 91 3.08e-31 105
Msa0765350 AT5G39860.1 65.385 78 26 1 17 93 15 92 1.80e-30 103

Find 64 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATAATAAAATTAAATT+TGG 0.194346 5_2:+26143085 None:intergenic
GCTAGACATTGTTATAATTA+AGG 0.199410 5_2:+26142841 None:intergenic
TATCTGTGTAGGGTTTAATT+TGG 0.245749 5_2:-26142018 Msa0765350:three_prime_UTR
TGACCGATTGTCACAACTTT+TGG 0.275530 5_2:-26142197 Msa0765350:CDS
GAGCGGCTAGCGCGAAGTTC+GGG 0.276939 5_2:+26142728 None:intergenic
GAGAGGGGAGAGAGGGAGAG+AGG 0.326546 5_2:+26143216 None:intergenic
GTGCTCAAGCAGCTATTATT+AGG 0.330670 5_2:-26142156 Msa0765350:CDS
TGAAATGGGAGAGGGGAGAG+AGG 0.340488 5_2:+26143208 None:intergenic
ATTAATAGAGAGAATTGAAA+TGG 0.348642 5_2:+26143193 None:intergenic
GGAGCGGCTAGCGCGAAGTT+CGG 0.362273 5_2:+26142727 None:intergenic
GAAGTTATGTGAAATGAGTA+TGG 0.366690 5_2:+26142990 None:intergenic
GTTATTCTCTTATCTGTGTA+GGG 0.379197 5_2:-26142028 Msa0765350:three_prime_UTR
CTTATAGGTTTCAGCTACTA+AGG 0.382941 5_2:-26142278 Msa0765350:intron
TGTTATTCTCTTATCTGTGT+AGG 0.387894 5_2:-26142029 Msa0765350:three_prime_UTR
AGAGGAACCACCGGATTGTC+TGG 0.389390 5_2:+26142805 None:intergenic
GGGGATAAGTTGTTGTAACT+TGG 0.418381 5_2:+26142748 None:intergenic
GGAGGAGAGTGAAGAGGAGT+GGG 0.429020 5_2:+26143111 None:intergenic
AGAGGGGAGAGAGGGAGAGA+GGG 0.438829 5_2:+26143217 None:intergenic
TTAATAGAGAGAATTGAAAT+GGG 0.457141 5_2:+26143194 None:intergenic
GGTTTGTAGACCAAGGCAAA+AGG 0.461588 5_2:-26141997 Msa0765350:three_prime_UTR
AGAGAGGAAATGAGGTTGGT+TGG 0.463003 5_2:+26142938 None:intergenic
GAAATGGGAGAGGGGAGAGA+GGG 0.465674 5_2:+26143209 None:intergenic
AAATAAACAAAGAGAAAGAG+AGG 0.468720 5_2:+26142922 None:intergenic
GATAATAAAATTAAATTTGG+AGG 0.476768 5_2:+26143088 None:intergenic
AGAAAGAGAGGAAATGAGGT+TGG 0.479603 5_2:+26142934 None:intergenic
GGTTGGTTGGTTGTCAGAGA+AGG 0.483101 5_2:+26142951 None:intergenic
CACATTCAGAAAGGACAGTA+TGG 0.483265 5_2:-26143250 Msa0765350:five_prime_UTR
ATAATAAAATTAAATTTGGA+GGG 0.484167 5_2:+26143089 None:intergenic
AGAGAATTGAAATGGGAGAG+GGG 0.488069 5_2:+26143201 None:intergenic
AAAGAGAAAGAGAGGAAATG+AGG 0.488212 5_2:+26142930 None:intergenic
AAGAGAGAAATAAATAAAAG+TGG 0.494133 5_2:+26143034 None:intergenic
GAGGGAGAGAGGGAGAGATT+AGG 0.495619 5_2:+26143227 None:intergenic
AATAGCTGCTTGAGCACTGT+TGG 0.496664 5_2:+26142162 None:intergenic
TGTTATAATTAAGGAGGTAG+TGG 0.505098 5_2:+26142850 None:intergenic
TAAAATTAAATTTGGAGGGG+AGG 0.505190 5_2:+26143093 None:intergenic
TGTGAAATGAGTATGGAGTA+AGG 0.505231 5_2:+26142997 None:intergenic
TGGGAGAAAGAAGTGGGCTA+TGG 0.508176 5_2:+26143165 None:intergenic
GAGAGAATTGAAATGGGAGA+GGG 0.524632 5_2:+26143200 None:intergenic
AAGCCAAAAGTTGTGACAAT+CGG 0.524888 5_2:+26142194 None:intergenic
AGAGAGAATTGAAATGGGAG+AGG 0.526053 5_2:+26143199 None:intergenic
GTTTGTAGACCAAGGCAAAA+GGG 0.528021 5_2:-26141996 Msa0765350:three_prime_UTR
AAAATTAAACTAATTAATCA+AGG 0.530033 5_2:+26141948 None:intergenic
AGATCTCGTTCCAGACAATC+CGG 0.532260 5_2:-26142815 Msa0765350:CDS
GGGAGGAGAGTGAAGAGGAG+TGG 0.537209 5_2:+26143110 None:intergenic
GGGAGAAAGAAGTGGGCTAT+GGG 0.538681 5_2:+26143166 None:intergenic
TGGAGGGGAGGAGAGTGAAG+AGG 0.555226 5_2:+26143105 None:intergenic
GTGAAATGAGTATGGAGTAA+GGG 0.559416 5_2:+26142998 None:intergenic
AATTGAAGAGGACAAGTGCA+TGG 0.569802 5_2:+26143145 None:intergenic
GTTGAATTGTTGAATTGAAG+AGG 0.578890 5_2:+26143133 None:intergenic
TTAATTTGGTTTGTAGACCA+AGG 0.581266 5_2:-26142004 Msa0765350:three_prime_UTR
TAATAAAATTAAATTTGGAG+GGG 0.593264 5_2:+26143090 None:intergenic
AAATAAATACCTTGTTGGAG+CGG 0.605762 5_2:+26142711 None:intergenic
ATTGAAGAGGACAAGTGCAT+GGG 0.605864 5_2:+26143146 None:intergenic
AAGTGCATGGGAGAAAGAAG+TGG 0.606928 5_2:+26143158 None:intergenic
AGACATTGTTATAATTAAGG+AGG 0.612299 5_2:+26142844 None:intergenic
TCGCGCTAGCCGCTCCAACA+AGG 0.613710 5_2:-26142720 Msa0765350:intron
GTTAGGGGACACATTCAGAA+AGG 0.623167 5_2:-26143259 None:intergenic
AGTGATCTCAGAGGAACCAC+CGG 0.628272 5_2:+26142796 None:intergenic
AGTGCATGGGAGAAAGAAGT+GGG 0.640201 5_2:+26143159 None:intergenic
AGCGGCTAGCGCGAAGTTCG+GGG 0.650397 5_2:+26142729 None:intergenic
AATTAATCAAGGACTAACAA+AGG 0.658512 5_2:+26141959 None:intergenic
AATAACAATAAAGTGAGACA+AGG 0.708908 5_2:+26142045 None:intergenic
TCTCGTTCCAGACAATCCGG+TGG 0.728257 5_2:-26142812 Msa0765350:CDS
TTGAGCATCAGTGATCTCAG+AGG 0.728981 5_2:+26142787 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAAAATTAAATTTGGA+GGG + chr5_2:26142019-26142038 None:intergenic 10.0%
!! AAAATTAAACTAATTAATCA+AGG + chr5_2:26143160-26143179 None:intergenic 10.0%
!! ATAATAAAATTAAATTTGGA+GGG + chr5_2:26142019-26142038 None:intergenic 10.0%
!! AAAATTAAACTAATTAATCA+AGG + chr5_2:26143160-26143179 None:intergenic 10.0%
!! TAATAAAATTAAATTTGGAG+GGG + chr5_2:26142018-26142037 None:intergenic 15.0%
!! GATAATAAAATTAAATTTGG+AGG + chr5_2:26142020-26142039 None:intergenic 15.0%
!!! ATTTTTTTTTATGGTCTTAT+AGG - chr5_2:26142812-26142831 Msa0765350:CDS 15.0%
!! TAATAAAATTAAATTTGGAG+GGG + chr5_2:26142018-26142037 None:intergenic 15.0%
!! GATAATAAAATTAAATTTGG+AGG + chr5_2:26142020-26142039 None:intergenic 15.0%
!!! ATTTTTTTTTATGGTCTTAT+AGG - chr5_2:26142812-26142831 Msa0765350:CDS 15.0%
!! TTAATAGAGAGAATTGAAAT+GGG + chr5_2:26141914-26141933 None:intergenic 20.0%
!! ATTAATAGAGAGAATTGAAA+TGG + chr5_2:26141915-26141934 None:intergenic 20.0%
!! AAGAGAGAAATAAATAAAAG+TGG + chr5_2:26142074-26142093 None:intergenic 20.0%
!! TAGAAAAATAAATACCTTGT+TGG + chr5_2:26142402-26142421 None:intergenic 20.0%
!! TTAATAGAGAGAATTGAAAT+GGG + chr5_2:26141914-26141933 None:intergenic 20.0%
!! ATTAATAGAGAGAATTGAAA+TGG + chr5_2:26141915-26141934 None:intergenic 20.0%
!! AAGAGAGAAATAAATAAAAG+TGG + chr5_2:26142074-26142093 None:intergenic 20.0%
!! TAGAAAAATAAATACCTTGT+TGG + chr5_2:26142402-26142421 None:intergenic 20.0%
!!! TGTTTTTTGTAATGTTGCTA+AGG + chr5_2:26142046-26142065 None:intergenic 25.0%
! AAATAAACAAAGAGAAAGAG+AGG + chr5_2:26142186-26142205 None:intergenic 25.0%
! AGACATTGTTATAATTAAGG+AGG + chr5_2:26142264-26142283 None:intergenic 25.0%
! GCTAGACATTGTTATAATTA+AGG + chr5_2:26142267-26142286 None:intergenic 25.0%
! AATAACAATAAAGTGAGACA+AGG + chr5_2:26143063-26143082 None:intergenic 25.0%
! AATTAATCAAGGACTAACAA+AGG + chr5_2:26143149-26143168 None:intergenic 25.0%
!!! TGTTTTTTGTAATGTTGCTA+AGG + chr5_2:26142046-26142065 None:intergenic 25.0%
! AAATAAACAAAGAGAAAGAG+AGG + chr5_2:26142186-26142205 None:intergenic 25.0%
! AGACATTGTTATAATTAAGG+AGG + chr5_2:26142264-26142283 None:intergenic 25.0%
! GCTAGACATTGTTATAATTA+AGG + chr5_2:26142267-26142286 None:intergenic 25.0%
! AATAACAATAAAGTGAGACA+AGG + chr5_2:26143063-26143082 None:intergenic 25.0%
! AATTAATCAAGGACTAACAA+AGG + chr5_2:26143149-26143168 None:intergenic 25.0%
GTTGAATTGTTGAATTGAAG+AGG + chr5_2:26141975-26141994 None:intergenic 30.0%
TAAAATTAAATTTGGAGGGG+AGG + chr5_2:26142015-26142034 None:intergenic 30.0%
GAAGTTATGTGAAATGAGTA+TGG + chr5_2:26142118-26142137 None:intergenic 30.0%
TGTTATAATTAAGGAGGTAG+TGG + chr5_2:26142258-26142277 None:intergenic 30.0%
AAATAAATACCTTGTTGGAG+CGG + chr5_2:26142397-26142416 None:intergenic 30.0%
ACAATACAATACACAAGTTG+TGG + chr5_2:26142659-26142678 None:intergenic 30.0%
CATAAAAAACTTGCATAGAG+AGG - chr5_2:26142872-26142891 Msa0765350:five_prime_UTR 30.0%
TGTTATTCTCTTATCTGTGT+AGG - chr5_2:26143076-26143095 Msa0765350:five_prime_UTR 30.0%
GTTATTCTCTTATCTGTGTA+GGG - chr5_2:26143077-26143096 Msa0765350:five_prime_UTR 30.0%
TATCTGTGTAGGGTTTAATT+TGG - chr5_2:26143087-26143106 Msa0765350:five_prime_UTR 30.0%
!! TTAATTTGGTTTGTAGACCA+AGG - chr5_2:26143101-26143120 Msa0765350:five_prime_UTR 30.0%
GTTGAATTGTTGAATTGAAG+AGG + chr5_2:26141975-26141994 None:intergenic 30.0%
TAAAATTAAATTTGGAGGGG+AGG + chr5_2:26142015-26142034 None:intergenic 30.0%
GAAGTTATGTGAAATGAGTA+TGG + chr5_2:26142118-26142137 None:intergenic 30.0%
TGTTATAATTAAGGAGGTAG+TGG + chr5_2:26142258-26142277 None:intergenic 30.0%
AAATAAATACCTTGTTGGAG+CGG + chr5_2:26142397-26142416 None:intergenic 30.0%
ACAATACAATACACAAGTTG+TGG + chr5_2:26142659-26142678 None:intergenic 30.0%
CATAAAAAACTTGCATAGAG+AGG - chr5_2:26142872-26142891 Msa0765350:five_prime_UTR 30.0%
TGTTATTCTCTTATCTGTGT+AGG - chr5_2:26143076-26143095 Msa0765350:five_prime_UTR 30.0%
GTTATTCTCTTATCTGTGTA+GGG - chr5_2:26143077-26143096 Msa0765350:five_prime_UTR 30.0%
TATCTGTGTAGGGTTTAATT+TGG - chr5_2:26143087-26143106 Msa0765350:five_prime_UTR 30.0%
!! TTAATTTGGTTTGTAGACCA+AGG - chr5_2:26143101-26143120 Msa0765350:five_prime_UTR 30.0%
GTGAAATGAGTATGGAGTAA+GGG + chr5_2:26142110-26142129 None:intergenic 35.0%
TGTGAAATGAGTATGGAGTA+AGG + chr5_2:26142111-26142130 None:intergenic 35.0%
AAAGAGAAAGAGAGGAAATG+AGG + chr5_2:26142178-26142197 None:intergenic 35.0%
! ATTTGTGAGAGTGTGTATGA+AGG + chr5_2:26142521-26142540 None:intergenic 35.0%
GATACAATGCACATATTGTG+TGG - chr5_2:26142576-26142595 Msa0765350:intron 35.0%
!! TGTGTGGACATTGTTAGTTT+TGG - chr5_2:26142592-26142611 Msa0765350:intron 35.0%
CTTATAGGTTTCAGCTACTA+AGG - chr5_2:26142827-26142846 Msa0765350:CDS 35.0%
AAGCCAAAAGTTGTGACAAT+CGG + chr5_2:26142914-26142933 None:intergenic 35.0%
GTGAAATGAGTATGGAGTAA+GGG + chr5_2:26142110-26142129 None:intergenic 35.0%
TGTGAAATGAGTATGGAGTA+AGG + chr5_2:26142111-26142130 None:intergenic 35.0%
AAAGAGAAAGAGAGGAAATG+AGG + chr5_2:26142178-26142197 None:intergenic 35.0%
! ATTTGTGAGAGTGTGTATGA+AGG + chr5_2:26142521-26142540 None:intergenic 35.0%
GATACAATGCACATATTGTG+TGG - chr5_2:26142576-26142595 Msa0765350:intron 35.0%
!! TGTGTGGACATTGTTAGTTT+TGG - chr5_2:26142592-26142611 Msa0765350:intron 35.0%
CTTATAGGTTTCAGCTACTA+AGG - chr5_2:26142827-26142846 Msa0765350:CDS 35.0%
AAGCCAAAAGTTGTGACAAT+CGG + chr5_2:26142914-26142933 None:intergenic 35.0%
CACATTCAGAAAGGACAGTA+TGG - chr5_2:26141855-26141874 Msa0765350:three_prime_UTR 40.0%
AGAGAATTGAAATGGGAGAG+GGG + chr5_2:26141907-26141926 None:intergenic 40.0%
GAGAGAATTGAAATGGGAGA+GGG + chr5_2:26141908-26141927 None:intergenic 40.0%
AGAGAGAATTGAAATGGGAG+AGG + chr5_2:26141909-26141928 None:intergenic 40.0%
ATTGAAGAGGACAAGTGCAT+GGG + chr5_2:26141962-26141981 None:intergenic 40.0%
AATTGAAGAGGACAAGTGCA+TGG + chr5_2:26141963-26141982 None:intergenic 40.0%
AGAAAGAGAGGAAATGAGGT+TGG + chr5_2:26142174-26142193 None:intergenic 40.0%
GGGGATAAGTTGTTGTAACT+TGG + chr5_2:26142360-26142379 None:intergenic 40.0%
GTGCATGTGAATGATGACTT+GGG + chr5_2:26142545-26142564 None:intergenic 40.0%
TGTGCATGTGAATGATGACT+TGG + chr5_2:26142546-26142565 None:intergenic 40.0%
CAAGTTGTGGCAAAGAATTG+AGG + chr5_2:26142646-26142665 None:intergenic 40.0%
! TGACCGATTGTCACAACTTT+TGG - chr5_2:26142908-26142927 Msa0765350:five_prime_UTR 40.0%
GTGCTCAAGCAGCTATTATT+AGG - chr5_2:26142949-26142968 Msa0765350:five_prime_UTR 40.0%
GTTTGTAGACCAAGGCAAAA+GGG - chr5_2:26143109-26143128 Msa0765350:five_prime_UTR 40.0%
! TATAGTAAGCCCTTTTGCCT+TGG + chr5_2:26143121-26143140 None:intergenic 40.0%
CACATTCAGAAAGGACAGTA+TGG - chr5_2:26141855-26141874 Msa0765350:three_prime_UTR 40.0%
AGAGAATTGAAATGGGAGAG+GGG + chr5_2:26141907-26141926 None:intergenic 40.0%
GAGAGAATTGAAATGGGAGA+GGG + chr5_2:26141908-26141927 None:intergenic 40.0%
AGAGAGAATTGAAATGGGAG+AGG + chr5_2:26141909-26141928 None:intergenic 40.0%
ATTGAAGAGGACAAGTGCAT+GGG + chr5_2:26141962-26141981 None:intergenic 40.0%
AATTGAAGAGGACAAGTGCA+TGG + chr5_2:26141963-26141982 None:intergenic 40.0%
AGAAAGAGAGGAAATGAGGT+TGG + chr5_2:26142174-26142193 None:intergenic 40.0%
GGGGATAAGTTGTTGTAACT+TGG + chr5_2:26142360-26142379 None:intergenic 40.0%
GTGCATGTGAATGATGACTT+GGG + chr5_2:26142545-26142564 None:intergenic 40.0%
TGTGCATGTGAATGATGACT+TGG + chr5_2:26142546-26142565 None:intergenic 40.0%
CAAGTTGTGGCAAAGAATTG+AGG + chr5_2:26142646-26142665 None:intergenic 40.0%
! TGACCGATTGTCACAACTTT+TGG - chr5_2:26142908-26142927 Msa0765350:five_prime_UTR 40.0%
GTGCTCAAGCAGCTATTATT+AGG - chr5_2:26142949-26142968 Msa0765350:five_prime_UTR 40.0%
GTTTGTAGACCAAGGCAAAA+GGG - chr5_2:26143109-26143128 Msa0765350:five_prime_UTR 40.0%
! TATAGTAAGCCCTTTTGCCT+TGG + chr5_2:26143121-26143140 None:intergenic 40.0%
AGTGCATGGGAGAAAGAAGT+GGG + chr5_2:26141949-26141968 None:intergenic 45.0%
AAGTGCATGGGAGAAAGAAG+TGG + chr5_2:26141950-26141969 None:intergenic 45.0%
! AGAGAGGAAATGAGGTTGGT+TGG + chr5_2:26142170-26142189 None:intergenic 45.0%
AGATCTCGTTCCAGACAATC+CGG - chr5_2:26142290-26142309 Msa0765350:intron 45.0%
TTGAGCATCAGTGATCTCAG+AGG + chr5_2:26142321-26142340 None:intergenic 45.0%
AGCTAGTTGCAACATGCAAG+TGG - chr5_2:26142725-26142744 Msa0765350:CDS 45.0%
GCTAGTTGCAACATGCAAGT+GGG - chr5_2:26142726-26142745 Msa0765350:CDS 45.0%
! AATAGCTGCTTGAGCACTGT+TGG + chr5_2:26142946-26142965 None:intergenic 45.0%
GGTTTGTAGACCAAGGCAAA+AGG - chr5_2:26143108-26143127 Msa0765350:five_prime_UTR 45.0%
AGTGCATGGGAGAAAGAAGT+GGG + chr5_2:26141949-26141968 None:intergenic 45.0%
AAGTGCATGGGAGAAAGAAG+TGG + chr5_2:26141950-26141969 None:intergenic 45.0%
! AGAGAGGAAATGAGGTTGGT+TGG + chr5_2:26142170-26142189 None:intergenic 45.0%
AGATCTCGTTCCAGACAATC+CGG - chr5_2:26142290-26142309 Msa0765350:intron 45.0%
TTGAGCATCAGTGATCTCAG+AGG + chr5_2:26142321-26142340 None:intergenic 45.0%
AGCTAGTTGCAACATGCAAG+TGG - chr5_2:26142725-26142744 Msa0765350:CDS 45.0%
GCTAGTTGCAACATGCAAGT+GGG - chr5_2:26142726-26142745 Msa0765350:CDS 45.0%
! AATAGCTGCTTGAGCACTGT+TGG + chr5_2:26142946-26142965 None:intergenic 45.0%
GGTTTGTAGACCAAGGCAAA+AGG - chr5_2:26143108-26143127 Msa0765350:five_prime_UTR 45.0%
!! TATGATAATAAAATTAAATT+TGG + chr5_2:26142023-26142042 None:intergenic 5.0%
!!! TTAATTTGTATTTTTTTTTA+TGG - chr5_2:26142803-26142822 Msa0765350:CDS 5.0%
!! TATGATAATAAAATTAAATT+TGG + chr5_2:26142023-26142042 None:intergenic 5.0%
!!! TTAATTTGTATTTTTTTTTA+TGG - chr5_2:26142803-26142822 Msa0765350:CDS 5.0%
!! GGGAGAAAGAAGTGGGCTAT+GGG + chr5_2:26141942-26141961 None:intergenic 50.0%
!! TGGGAGAAAGAAGTGGGCTA+TGG + chr5_2:26141943-26141962 None:intergenic 50.0%
! GGTTGGTTGGTTGTCAGAGA+AGG + chr5_2:26142157-26142176 None:intergenic 50.0%
AGTGATCTCAGAGGAACCAC+CGG + chr5_2:26142312-26142331 None:intergenic 50.0%
AGTTGCAACATGCAAGTGGG+TGG - chr5_2:26142729-26142748 Msa0765350:CDS 50.0%
!! GGGAGAAAGAAGTGGGCTAT+GGG + chr5_2:26141942-26141961 None:intergenic 50.0%
!! TGGGAGAAAGAAGTGGGCTA+TGG + chr5_2:26141943-26141962 None:intergenic 50.0%
! GGTTGGTTGGTTGTCAGAGA+AGG + chr5_2:26142157-26142176 None:intergenic 50.0%
AGTGATCTCAGAGGAACCAC+CGG + chr5_2:26142312-26142331 None:intergenic 50.0%
AGTTGCAACATGCAAGTGGG+TGG - chr5_2:26142729-26142748 Msa0765350:CDS 50.0%
GAGGGAGAGAGGGAGAGATT+AGG + chr5_2:26141881-26141900 None:intergenic 55.0%
GAAATGGGAGAGGGGAGAGA+GGG + chr5_2:26141899-26141918 None:intergenic 55.0%
TGAAATGGGAGAGGGGAGAG+AGG + chr5_2:26141900-26141919 None:intergenic 55.0%
GGAGGAGAGTGAAGAGGAGT+GGG + chr5_2:26141997-26142016 None:intergenic 55.0%
TCTCGTTCCAGACAATCCGG+TGG - chr5_2:26142293-26142312 Msa0765350:intron 55.0%
AGAGGAACCACCGGATTGTC+TGG + chr5_2:26142303-26142322 None:intergenic 55.0%
GAGGGAGAGAGGGAGAGATT+AGG + chr5_2:26141881-26141900 None:intergenic 55.0%
GAAATGGGAGAGGGGAGAGA+GGG + chr5_2:26141899-26141918 None:intergenic 55.0%
TGAAATGGGAGAGGGGAGAG+AGG + chr5_2:26141900-26141919 None:intergenic 55.0%
GGAGGAGAGTGAAGAGGAGT+GGG + chr5_2:26141997-26142016 None:intergenic 55.0%
TCTCGTTCCAGACAATCCGG+TGG - chr5_2:26142293-26142312 Msa0765350:intron 55.0%
AGAGGAACCACCGGATTGTC+TGG + chr5_2:26142303-26142322 None:intergenic 55.0%
AGAGGGGAGAGAGGGAGAGA+GGG + chr5_2:26141891-26141910 None:intergenic 60.0%
GGGAGGAGAGTGAAGAGGAG+TGG + chr5_2:26141998-26142017 None:intergenic 60.0%
TGGAGGGGAGGAGAGTGAAG+AGG + chr5_2:26142003-26142022 None:intergenic 60.0%
AGAGGGGAGAGAGGGAGAGA+GGG + chr5_2:26141891-26141910 None:intergenic 60.0%
GGGAGGAGAGTGAAGAGGAG+TGG + chr5_2:26141998-26142017 None:intergenic 60.0%
TGGAGGGGAGGAGAGTGAAG+AGG + chr5_2:26142003-26142022 None:intergenic 60.0%
GAGAGGGGAGAGAGGGAGAG+AGG + chr5_2:26141892-26141911 None:intergenic 65.0%
AGCGGCTAGCGCGAAGTTCG+GGG + chr5_2:26142379-26142398 None:intergenic 65.0%
GAGCGGCTAGCGCGAAGTTC+GGG + chr5_2:26142380-26142399 None:intergenic 65.0%
GGAGCGGCTAGCGCGAAGTT+CGG + chr5_2:26142381-26142400 None:intergenic 65.0%
TCGCGCTAGCCGCTCCAACA+AGG - chr5_2:26142385-26142404 Msa0765350:intron 65.0%
GAGAGGGGAGAGAGGGAGAG+AGG + chr5_2:26141892-26141911 None:intergenic 65.0%
AGCGGCTAGCGCGAAGTTCG+GGG + chr5_2:26142379-26142398 None:intergenic 65.0%
GAGCGGCTAGCGCGAAGTTC+GGG + chr5_2:26142380-26142399 None:intergenic 65.0%
GGAGCGGCTAGCGCGAAGTT+CGG + chr5_2:26142381-26142400 None:intergenic 65.0%
TCGCGCTAGCCGCTCCAACA+AGG - chr5_2:26142385-26142404 Msa0765350:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 26141853 26143274 26141853 ID=Msa0765350;Name=Msa0765350
chr5_2 mRNA 26141853 26143274 26141853 ID=Msa0765350-mRNA-1;Parent=Msa0765350;Name=Msa0765350-mRNA-1;_AED=0.02;_eAED=0.02;_QI=425|1|1|1|1|1|2|288|93
chr5_2 exon 26141853 26142293 26141853 ID=Msa0765350-mRNA-1:exon:10419;Parent=Msa0765350-mRNA-1
chr5_2 exon 26142721 26143274 26142721 ID=Msa0765350-mRNA-1:exon:10418;Parent=Msa0765350-mRNA-1
chr5_2 five_prime_UTR 26142850 26143274 26142850 ID=Msa0765350-mRNA-1:five_prime_utr;Parent=Msa0765350-mRNA-1
chr5_2 CDS 26142721 26142849 26142721 ID=Msa0765350-mRNA-1:cds;Parent=Msa0765350-mRNA-1
chr5_2 CDS 26142141 26142293 26142141 ID=Msa0765350-mRNA-1:cds;Parent=Msa0765350-mRNA-1
chr5_2 three_prime_UTR 26141853 26142140 26141853 ID=Msa0765350-mRNA-1:three_prime_utr;Parent=Msa0765350-mRNA-1
Gene Sequence

>Msa0765350

ATGTCTAGCAGAAGATCTCGTTCCAGACAATCCGGTGGTTCCTCTGAGATCACTGATGCTCAAATCACTGATCTCATTTCCAAGTTACAACAACTTATCCCCGAACTTCGCGCTAGCCGCTCCAACAAGGTTTCAGCTACTAAGGTATTGCAAGAGACTTGCAACTACATAAAAAACTTGCATAGAGAGGTTGATGATTTAAGTGACCGATTGTCACAACTTTTGGCTTCTACAGATTCCAACAGTGCTCAAGCAGCTATTATTAGGAGCTTACTTATGTAA
Protein sequence

>Msa0765350

MSSRRSRSRQSGGSSEITDAQITDLISKLQQLIPELRASRSNKVSATKVLQETCNYIKNLHREVDDLSDRLSQLLASTDSNSAQAAIIRSLLM*