Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772750 | XP_003611805.1 | 80.220 | 91 | 15 | 1 | 1 | 88 | 108 | 198 | 9.98e-37 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772750 | G7K838 | 80.220 | 91 | 15 | 1 | 1 | 88 | 108 | 198 | 4.77e-37 | 142 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0772750 | Msa0828660 | 0.807323 | 5.345911e-50 | -8.615850e-47 |
| Msa0308460 | Msa0772750 | 0.806759 | 7.037386e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0772750 | MtrunA17_Chr5g0402671 | 80.220 | 91 | 15 | 1 | 1 | 88 | 108 | 198 | 9.18e-41 | 142 |
| Msa0772750 | MtrunA17_Chr5g0403291 | 76.000 | 75 | 15 | 1 | 14 | 88 | 1 | 72 | 2.10e-30 | 112 |
| Msa0772750 | MtrunA17_Chr5g0402771 | 66.667 | 87 | 26 | 1 | 2 | 88 | 113 | 196 | 8.55e-29 | 107 |
| Msa0772750 | MtrunA17_Chr5g0402761 | 61.446 | 83 | 27 | 2 | 1 | 78 | 112 | 194 | 9.60e-24 | 91.3 |
| Msa0772750 | MtrunA17_Chr5g0402751 | 71.212 | 66 | 14 | 2 | 22 | 86 | 12 | 73 | 2.55e-23 | 88.2 |
| Msa0772750 | MtrunA17_Chr5g0402721 | 54.762 | 84 | 25 | 2 | 5 | 88 | 113 | 183 | 3.00e-19 | 80.9 |
| Msa0772750 | MtrunA17_Chr5g0402681 | 52.381 | 84 | 27 | 2 | 5 | 88 | 112 | 182 | 1.69e-17 | 75.9 |
| Msa0772750 | MtrunA17_Chr5g0402701 | 48.276 | 87 | 32 | 1 | 2 | 88 | 113 | 186 | 1.38e-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATGAGAAAGGAGAATT+TGG | 0.149852 | 5_2:+47101558 | Msa0772750:CDS |
| CCCATACACTGAGGCTCTTC+AGG | 0.316163 | 5_2:-47101622 | None:intergenic |
| GAGAATTTGGGTCAGTTTGA+TGG | 0.352961 | 5_2:+47101571 | Msa0772750:CDS |
| ACCTCTTGTTATGGTTGGAT+TGG | 0.359455 | 5_2:+47102241 | Msa0772750:CDS |
| TTTGATGGGAATCAAATTAG+AGG | 0.369090 | 5_2:+47101586 | Msa0772750:CDS |
| TTGACTGGTTTGGGTGGATC+TGG | 0.382247 | 5_2:+47101709 | Msa0772750:CDS |
| TCCTGAAGAGCCTCAGTGTA+TGG | 0.405402 | 5_2:+47101621 | Msa0772750:CDS |
| TTGATGAGAAAGGAGAATTT+GGG | 0.406265 | 5_2:+47101559 | Msa0772750:CDS |
| TAGCATCTAACCTCTTGTTA+TGG | 0.421873 | 5_2:+47102232 | Msa0772750:intron |
| AAGATTCAATTATTGAAAGA+TGG | 0.434309 | 5_2:+47101670 | Msa0772750:CDS |
| CTTGTTATGGTTGGATTGGA+TGG | 0.445217 | 5_2:+47102245 | Msa0772750:CDS |
| ATCTAACCTCTTGTTATGGT+TGG | 0.448764 | 5_2:+47102236 | Msa0772750:CDS |
| AGAATTTGGGTCAGTTTGAT+GGG | 0.460636 | 5_2:+47101572 | Msa0772750:CDS |
| TGAATCTTCAACTTATTCAA+AGG | 0.506687 | 5_2:-47101655 | None:intergenic |
| GAATAAAAGAGATTTGATGA+AGG | 0.530756 | 5_2:+47101504 | Msa0772750:CDS |
| CCTGAAGAGCCTCAGTGTAT+GGG | 0.544956 | 5_2:+47101622 | Msa0772750:CDS |
| TCCAATCCAACCATAACAAG+AGG | 0.590848 | 5_2:-47102242 | None:intergenic |
| AATCAAATTAGAGGTTTGTG+TGG | 0.668031 | 5_2:+47101595 | Msa0772750:CDS |
| TCATCAACTCCCATACACTG+AGG | 0.781410 | 5_2:-47101631 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATTCAATTATTGAAAGA+TGG | + | chr5_2:47101670-47101689 | Msa0772750:CDS | 20.0% |
| !!! | TAAATTATTGCTACTCTTTT+AGG | + | chr5_2:47101813-47101832 | Msa0772750:intron | 20.0% |
| !!! | AAATTATTGCTACTCTTTTA+GGG | + | chr5_2:47101814-47101833 | Msa0772750:intron | 20.0% |
| !! | AAGATTCAATTATTGAAAGA+TGG | + | chr5_2:47101670-47101689 | Msa0772750:CDS | 20.0% |
| !!! | TAAATTATTGCTACTCTTTT+AGG | + | chr5_2:47101813-47101832 | Msa0772750:intron | 20.0% |
| !!! | AAATTATTGCTACTCTTTTA+GGG | + | chr5_2:47101814-47101833 | Msa0772750:intron | 20.0% |
| !! | AAATCTCTTTTATTCTCAAC+TGG | - | chr5_2:47101499-47101518 | None:intergenic | 25.0% |
| ! | GAATAAAAGAGATTTGATGA+AGG | + | chr5_2:47101504-47101523 | Msa0772750:CDS | 25.0% |
| !!! | TTTGATGAAGGTTTTGAATA+AGG | + | chr5_2:47101516-47101535 | Msa0772750:CDS | 25.0% |
| ! | TGAATCTTCAACTTATTCAA+AGG | - | chr5_2:47101658-47101677 | None:intergenic | 25.0% |
| ! | AAAGAGTAGCAATAATTTAG+TGG | - | chr5_2:47101812-47101831 | None:intergenic | 25.0% |
| !! | ACTTATTTGAGATTATATGC+TGG | + | chr5_2:47101853-47101872 | Msa0772750:intron | 25.0% |
| !! | AAATCTCTTTTATTCTCAAC+TGG | - | chr5_2:47101499-47101518 | None:intergenic | 25.0% |
| ! | GAATAAAAGAGATTTGATGA+AGG | + | chr5_2:47101504-47101523 | Msa0772750:CDS | 25.0% |
| !!! | TTTGATGAAGGTTTTGAATA+AGG | + | chr5_2:47101516-47101535 | Msa0772750:CDS | 25.0% |
| ! | TGAATCTTCAACTTATTCAA+AGG | - | chr5_2:47101658-47101677 | None:intergenic | 25.0% |
| ! | AAAGAGTAGCAATAATTTAG+TGG | - | chr5_2:47101812-47101831 | None:intergenic | 25.0% |
| !! | ACTTATTTGAGATTATATGC+TGG | + | chr5_2:47101853-47101872 | Msa0772750:intron | 25.0% |
| !! | ACTTGAGATTTTGATGAGAA+AGG | + | chr5_2:47101549-47101568 | Msa0772750:CDS | 30.0% |
| TTTGATGAGAAAGGAGAATT+TGG | + | chr5_2:47101558-47101577 | Msa0772750:CDS | 30.0% | |
| TTGATGAGAAAGGAGAATTT+GGG | + | chr5_2:47101559-47101578 | Msa0772750:CDS | 30.0% | |
| TTTGATGGGAATCAAATTAG+AGG | + | chr5_2:47101586-47101605 | Msa0772750:CDS | 30.0% | |
| AATCAAATTAGAGGTTTGTG+TGG | + | chr5_2:47101595-47101614 | Msa0772750:CDS | 30.0% | |
| ACTTTGTGAGACTATTTAAG+AGG | + | chr5_2:47101883-47101902 | Msa0772750:intron | 30.0% | |
| CTTTGTGAGACTATTTAAGA+GGG | + | chr5_2:47101884-47101903 | Msa0772750:intron | 30.0% | |
| CAAGTAAGTTCACAAGATTA+AGG | - | chr5_2:47102168-47102187 | None:intergenic | 30.0% | |
| !! | ACTTGAGATTTTGATGAGAA+AGG | + | chr5_2:47101549-47101568 | Msa0772750:CDS | 30.0% |
| TTTGATGAGAAAGGAGAATT+TGG | + | chr5_2:47101558-47101577 | Msa0772750:CDS | 30.0% | |
| TTGATGAGAAAGGAGAATTT+GGG | + | chr5_2:47101559-47101578 | Msa0772750:CDS | 30.0% | |
| TTTGATGGGAATCAAATTAG+AGG | + | chr5_2:47101586-47101605 | Msa0772750:CDS | 30.0% | |
| AATCAAATTAGAGGTTTGTG+TGG | + | chr5_2:47101595-47101614 | Msa0772750:CDS | 30.0% | |
| ACTTTGTGAGACTATTTAAG+AGG | + | chr5_2:47101883-47101902 | Msa0772750:intron | 30.0% | |
| CTTTGTGAGACTATTTAAGA+GGG | + | chr5_2:47101884-47101903 | Msa0772750:intron | 30.0% | |
| CAAGTAAGTTCACAAGATTA+AGG | - | chr5_2:47102168-47102187 | None:intergenic | 30.0% | |
| !!! | GATGAAGGTTTTGAATAAGG+TGG | + | chr5_2:47101519-47101538 | Msa0772750:CDS | 35.0% |
| !!! | AAGGTTTTGAATAAGGTGGA+TGG | + | chr5_2:47101523-47101542 | Msa0772750:CDS | 35.0% |
| AGAATTTGGGTCAGTTTGAT+GGG | + | chr5_2:47101572-47101591 | Msa0772750:CDS | 35.0% | |
| !!! | GTTTCTGTACTTGTTTTGAC+TGG | + | chr5_2:47101694-47101713 | Msa0772750:CDS | 35.0% |
| !!! | TGTACTTGTTTTGACTGGTT+TGG | + | chr5_2:47101699-47101718 | Msa0772750:CDS | 35.0% |
| !!! | GTACTTGTTTTGACTGGTTT+GGG | + | chr5_2:47101700-47101719 | Msa0772750:CDS | 35.0% |
| !! | TAGTTACTACCTTTGACTTG+TGG | - | chr5_2:47101769-47101788 | None:intergenic | 35.0% |
| TAGCATCTAACCTCTTGTTA+TGG | + | chr5_2:47102232-47102251 | Msa0772750:intron | 35.0% | |
| ! | ATCTAACCTCTTGTTATGGT+TGG | + | chr5_2:47102236-47102255 | Msa0772750:CDS | 35.0% |
| !!! | GATGAAGGTTTTGAATAAGG+TGG | + | chr5_2:47101519-47101538 | Msa0772750:CDS | 35.0% |
| !!! | AAGGTTTTGAATAAGGTGGA+TGG | + | chr5_2:47101523-47101542 | Msa0772750:CDS | 35.0% |
| AGAATTTGGGTCAGTTTGAT+GGG | + | chr5_2:47101572-47101591 | Msa0772750:CDS | 35.0% | |
| !!! | GTTTCTGTACTTGTTTTGAC+TGG | + | chr5_2:47101694-47101713 | Msa0772750:CDS | 35.0% |
| !!! | TGTACTTGTTTTGACTGGTT+TGG | + | chr5_2:47101699-47101718 | Msa0772750:CDS | 35.0% |
| !!! | GTACTTGTTTTGACTGGTTT+GGG | + | chr5_2:47101700-47101719 | Msa0772750:CDS | 35.0% |
| !! | TAGTTACTACCTTTGACTTG+TGG | - | chr5_2:47101769-47101788 | None:intergenic | 35.0% |
| TAGCATCTAACCTCTTGTTA+TGG | + | chr5_2:47102232-47102251 | Msa0772750:intron | 35.0% | |
| ! | ATCTAACCTCTTGTTATGGT+TGG | + | chr5_2:47102236-47102255 | Msa0772750:CDS | 35.0% |
| GAGAATTTGGGTCAGTTTGA+TGG | + | chr5_2:47101571-47101590 | Msa0772750:CDS | 40.0% | |
| CTCTTGCTAAGAAACTGTGT+TGG | + | chr5_2:47101740-47101759 | Msa0772750:intron | 40.0% | |
| TGTTGGAATCCACAAGTCAA+AGG | + | chr5_2:47101757-47101776 | Msa0772750:intron | 40.0% | |
| CTCATGCAGACAGTTTCTAT+TGG | - | chr5_2:47101963-47101982 | None:intergenic | 40.0% | |
| ! | ACCTCTTGTTATGGTTGGAT+TGG | + | chr5_2:47102241-47102260 | Msa0772750:CDS | 40.0% |
| TCCAATCCAACCATAACAAG+AGG | - | chr5_2:47102245-47102264 | None:intergenic | 40.0% | |
| ! | CTTGTTATGGTTGGATTGGA+TGG | + | chr5_2:47102245-47102264 | Msa0772750:CDS | 40.0% |
| GAGAATTTGGGTCAGTTTGA+TGG | + | chr5_2:47101571-47101590 | Msa0772750:CDS | 40.0% | |
| CTCTTGCTAAGAAACTGTGT+TGG | + | chr5_2:47101740-47101759 | Msa0772750:intron | 40.0% | |
| TGTTGGAATCCACAAGTCAA+AGG | + | chr5_2:47101757-47101776 | Msa0772750:intron | 40.0% | |
| CTCATGCAGACAGTTTCTAT+TGG | - | chr5_2:47101963-47101982 | None:intergenic | 40.0% | |
| ! | ACCTCTTGTTATGGTTGGAT+TGG | + | chr5_2:47102241-47102260 | Msa0772750:CDS | 40.0% |
| TCCAATCCAACCATAACAAG+AGG | - | chr5_2:47102245-47102264 | None:intergenic | 40.0% | |
| ! | CTTGTTATGGTTGGATTGGA+TGG | + | chr5_2:47102245-47102264 | Msa0772750:CDS | 40.0% |
| TCATCAACTCCCATACACTG+AGG | - | chr5_2:47101634-47101653 | None:intergenic | 45.0% | |
| !!! | CTTGTTTTGACTGGTTTGGG+TGG | + | chr5_2:47101703-47101722 | Msa0772750:CDS | 45.0% |
| ATGAAGCTTCGTTGTTCCCT+GGG | - | chr5_2:47102018-47102037 | None:intergenic | 45.0% | |
| TATGAAGCTTCGTTGTTCCC+TGG | - | chr5_2:47102019-47102038 | None:intergenic | 45.0% | |
| AGGGAACAACGAAGCTTCAT+AGG | + | chr5_2:47102018-47102037 | Msa0772750:intron | 45.0% | |
| GGGAACAACGAAGCTTCATA+GGG | + | chr5_2:47102019-47102038 | Msa0772750:intron | 45.0% | |
| TCATCAACTCCCATACACTG+AGG | - | chr5_2:47101634-47101653 | None:intergenic | 45.0% | |
| !!! | CTTGTTTTGACTGGTTTGGG+TGG | + | chr5_2:47101703-47101722 | Msa0772750:CDS | 45.0% |
| ATGAAGCTTCGTTGTTCCCT+GGG | - | chr5_2:47102018-47102037 | None:intergenic | 45.0% | |
| TATGAAGCTTCGTTGTTCCC+TGG | - | chr5_2:47102019-47102038 | None:intergenic | 45.0% | |
| AGGGAACAACGAAGCTTCAT+AGG | + | chr5_2:47102018-47102037 | Msa0772750:intron | 45.0% | |
| GGGAACAACGAAGCTTCATA+GGG | + | chr5_2:47102019-47102038 | Msa0772750:intron | 45.0% | |
| TCCTGAAGAGCCTCAGTGTA+TGG | + | chr5_2:47101621-47101640 | Msa0772750:CDS | 50.0% | |
| CCTGAAGAGCCTCAGTGTAT+GGG | + | chr5_2:47101622-47101641 | Msa0772750:CDS | 50.0% | |
| !! | TTGACTGGTTTGGGTGGATC+TGG | + | chr5_2:47101709-47101728 | Msa0772750:CDS | 50.0% |
| TCCTGAAGAGCCTCAGTGTA+TGG | + | chr5_2:47101621-47101640 | Msa0772750:CDS | 50.0% | |
| CCTGAAGAGCCTCAGTGTAT+GGG | + | chr5_2:47101622-47101641 | Msa0772750:CDS | 50.0% | |
| !! | TTGACTGGTTTGGGTGGATC+TGG | + | chr5_2:47101709-47101728 | Msa0772750:CDS | 50.0% |
| CCCATACACTGAGGCTCTTC+AGG | - | chr5_2:47101625-47101644 | None:intergenic | 55.0% | |
| GTCTGCATGAGACTGTCCCT+TGG | + | chr5_2:47101972-47101991 | Msa0772750:intron | 55.0% | |
| GACTGTCCCTTGGTCTGTAC+TGG | + | chr5_2:47101982-47102001 | Msa0772750:intron | 55.0% | |
| GCTTCGTTGTTCCCTGGGAT+TGG | - | chr5_2:47102013-47102032 | None:intergenic | 55.0% | |
| CCCATACACTGAGGCTCTTC+AGG | - | chr5_2:47101625-47101644 | None:intergenic | 55.0% | |
| GTCTGCATGAGACTGTCCCT+TGG | + | chr5_2:47101972-47101991 | Msa0772750:intron | 55.0% | |
| GACTGTCCCTTGGTCTGTAC+TGG | + | chr5_2:47101982-47102001 | Msa0772750:intron | 55.0% | |
| GCTTCGTTGTTCCCTGGGAT+TGG | - | chr5_2:47102013-47102032 | None:intergenic | 55.0% | |
| GCTGCGCCAGTACAGACCAA+GGG | - | chr5_2:47101991-47102010 | None:intergenic | 60.0% | |
| TACTGGCGCAGCCAATCCCA+GGG | + | chr5_2:47101999-47102018 | Msa0772750:intron | 60.0% | |
| GCTGCGCCAGTACAGACCAA+GGG | - | chr5_2:47101991-47102010 | None:intergenic | 60.0% | |
| TACTGGCGCAGCCAATCCCA+GGG | + | chr5_2:47101999-47102018 | Msa0772750:intron | 60.0% | |
| GGCTGCGCCAGTACAGACCA+AGG | - | chr5_2:47101992-47102011 | None:intergenic | 65.0% | |
| ! | GTACTGGCGCAGCCAATCCC+AGG | + | chr5_2:47101998-47102017 | Msa0772750:intron | 65.0% |
| GGCTGCGCCAGTACAGACCA+AGG | - | chr5_2:47101992-47102011 | None:intergenic | 65.0% | |
| ! | GTACTGGCGCAGCCAATCCC+AGG | + | chr5_2:47101998-47102017 | Msa0772750:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 47101481 | 47102272 | 47101481 | ID=Msa0772750;Name=Msa0772750 |
| chr5_2 | mRNA | 47101481 | 47102272 | 47101481 | ID=Msa0772750-mRNA-1;Parent=Msa0772750;Name=Msa0772750-mRNA-1;_AED=0.54;_eAED=0.54;_QI=0|0|0|0.5|1|1|2|0|95 |
| chr5_2 | exon | 47101481 | 47101730 | 47101481 | ID=Msa0772750-mRNA-1:exon:14151;Parent=Msa0772750-mRNA-1 |
| chr5_2 | exon | 47102235 | 47102272 | 47102235 | ID=Msa0772750-mRNA-1:exon:14152;Parent=Msa0772750-mRNA-1 |
| chr5_2 | CDS | 47101481 | 47101730 | 47101481 | ID=Msa0772750-mRNA-1:cds;Parent=Msa0772750-mRNA-1 |
| chr5_2 | CDS | 47102235 | 47102272 | 47102235 | ID=Msa0772750-mRNA-1:cds;Parent=Msa0772750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
