AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0776700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGGTCCTTCTGGTGTTTC+TGG 0.124700 5_2:+55174816 Msa0776700:five_prime_UTR
ATGCTTCAAAGGCTTTAATT+TGG 0.197779 5_2:+55177211 Msa0776700:three_prime_UTR
TATTGATCTCAAGGGCATTT+TGG 0.225051 5_2:+55176506 Msa0776700:CDS
GTTTCAGTGGGTGCGGTTGC+GGG 0.252658 5_2:+55174987 Msa0776700:five_prime_UTR
AGAAGCAAACATATTATTCT+TGG 0.302545 5_2:+55176044 Msa0776700:intron
ATTGGAAGATTCGGCGATTC+CGG 0.316865 5_2:+55174756 Msa0776700:five_prime_UTR
CGTTTCAGTGGGTGCGGTTG+CGG 0.324867 5_2:+55174986 Msa0776700:five_prime_UTR
TGCTTCAAAGGCTTTAATTT+GGG 0.335991 5_2:+55177212 Msa0776700:three_prime_UTR
GGTTCCCAAAGTGGTCCTTC+TGG 0.339347 5_2:+55174807 Msa0776700:five_prime_UTR
GAGAGGAGAAGAAGAGGAAA+GGG 0.341564 5_2:+55174697 Msa0776700:five_prime_UTR
ATTGCAGCGAATTGAAAGTT+TGG 0.376587 5_2:+55174786 Msa0776700:five_prime_UTR
GGTCGAGGGAGAGGAGAAGA+AGG 0.387824 5_2:+55174655 Msa0776700:five_prime_UTR
GACAGAGAGGGTCGAGGGAG+AGG 0.394862 5_2:+55174646 Msa0776700:five_prime_UTR
GAACTCGTCGGCGGTGGGAT+TGG 0.406300 5_2:+55174738 Msa0776700:five_prime_UTR
AGAGGAGAAGAAGGAAAAGA+AGG 0.410127 5_2:+55174664 Msa0776700:five_prime_UTR
TTGGAATCTCCTGTTGGAGT+TGG 0.412688 5_2:+55176063 Msa0776700:five_prime_UTR
AATGCTCTTGTTCTATATGA+TGG 0.436461 5_2:-55176292 None:intergenic
TAAGAAAATCCAACTCCAAC+AGG 0.446307 5_2:-55176072 None:intergenic
AAGAAGGGAGATCGAGGGAG+AGG 0.448388 5_2:+55174680 Msa0776700:five_prime_UTR
TCTTAGTTGTGCATATTCAC+TGG 0.451439 5_2:-55176106 None:intergenic
AAGGAAAAGAAGGGAGATCG+AGG 0.451530 5_2:+55174674 Msa0776700:five_prime_UTR
GGCGGTGGGATTGGAAGATT+CGG 0.459688 5_2:+55174747 Msa0776700:five_prime_UTR
AACACCAGAAGGACCACTTT+GGG 0.461899 5_2:-55174811 None:intergenic
AAAGTTATGCAAAACAGAAC+GGG 0.471311 5_2:+55174624 None:intergenic
CGCAACCGCACCCACTGAAA+CGG 0.472266 5_2:-55174985 None:intergenic
TGCCCTTGAGATCAATATGA+AGG 0.472647 5_2:-55176500 None:intergenic
TATAAATCTGAAATTTATTG+TGG 0.478342 5_2:+55175910 Msa0776700:five_prime_UTR
GAGGAGAAGAAGGAAAAGAA+GGG 0.479598 5_2:+55174665 Msa0776700:five_prime_UTR
TTCCCTTCATATTGATCTCA+AGG 0.480182 5_2:+55176497 Msa0776700:CDS
ATGTAACTGAAACCTTGATT+CGG 0.484290 5_2:+55177166 Msa0776700:three_prime_UTR
TCGAGGGAGAGGAGAAGAAG+AGG 0.488850 5_2:+55174691 Msa0776700:five_prime_UTR
GGAGAGGAGAAGAAGAGGAA+AGG 0.489204 5_2:+55174696 Msa0776700:five_prime_UTR
GAACGGGACAGAGAGGGTCG+AGG 0.492278 5_2:+55174640 Msa0776700:five_prime_UTR
AAGAAGGAACTCGTCGGCGG+TGG 0.492399 5_2:+55174732 Msa0776700:five_prime_UTR
TTATTCTTGGAATCTCCTGT+TGG 0.504241 5_2:+55176057 Msa0776700:five_prime_UTR
ATAGCAGGAGAGAGCTATGC+AGG 0.504990 5_2:+55176319 Msa0776700:exon
AAACACCAGAAGGACCACTT+TGG 0.505388 5_2:-55174812 None:intergenic
TGCTTTCAGAAACCGAATCA+AGG 0.505557 5_2:-55177178 None:intergenic
AAATCTGAAATTTATTGTGG+TGG 0.513890 5_2:+55175913 Msa0776700:five_prime_UTR
ACCTTCCGTTTCAGTGGGTG+CGG 0.522220 5_2:+55174980 Msa0776700:five_prime_UTR
AGAGGAGAAGAAGAGGAAAG+GGG 0.522655 5_2:+55174698 Msa0776700:five_prime_UTR
ATTCGCTGCAATTTCGTCGC+CGG 0.524805 5_2:-55174775 None:intergenic
GGTTTCTGAAAGCAAGGGAA+TGG 0.525303 5_2:+55177187 Msa0776700:three_prime_UTR
GACGAGTTCCTTCTTCCACT+TGG 0.537558 5_2:-55174724 None:intergenic
AAGGGAATGGAATGCTTCAA+AGG 0.537693 5_2:+55177200 Msa0776700:three_prime_UTR
AACGGGACAGAGAGGGTCGA+GGG 0.541857 5_2:+55174641 Msa0776700:five_prime_UTR
TGAAAGTTTGGTTCCCAAAG+TGG 0.542379 5_2:+55174798 Msa0776700:five_prime_UTR
AGGAAAAGAAGGGAGATCGA+GGG 0.548885 5_2:+55174675 Msa0776700:five_prime_UTR
TGAGAAACCTTCCGTTTCAG+TGG 0.551187 5_2:+55174974 Msa0776700:five_prime_UTR
ATGATGAGTTCAGCAAGCTC+TGG 0.554264 5_2:-55176456 None:intergenic
GAAAGAATTCAGATTTAGCA+AGG 0.557793 5_2:+55177242 Msa0776700:three_prime_UTR
AAAAGTTATGCAAAACAGAA+CGG 0.565831 5_2:+55174623 None:intergenic
AGAAGGAACTCGTCGGCGGT+GGG 0.570077 5_2:+55174733 Msa0776700:five_prime_UTR
TGATTCGGTTTCTGAAAGCA+AGG 0.571047 5_2:+55177181 Msa0776700:three_prime_UTR
GCCCTTGAGATCAATATGAA+GGG 0.580105 5_2:-55176499 None:intergenic
TTGAGATCAATATGAAGGGA+AGG 0.580444 5_2:-55176495 None:intergenic
AGGGGTAACCAAGTGGAAGA+AGG 0.583488 5_2:+55174716 Msa0776700:five_prime_UTR
TACTTGCAGAATGTTCATCA+AGG 0.587245 5_2:+55177141 Msa0776700:intron
GATTCGGTTTCTGAAAGCAA+GGG 0.593415 5_2:+55177182 Msa0776700:three_prime_UTR
TCCCTTCATATTGATCTCAA+GGG 0.595442 5_2:+55176498 Msa0776700:CDS
CAAAACAGAACGGGACAGAG+AGG 0.603671 5_2:+55174633 Msa0776700:five_prime_UTR
GAGAAACCTTCCGTTTCAGT+GGG 0.609923 5_2:+55174975 Msa0776700:five_prime_UTR
TGAGATCAATATGAAGGGAA+GGG 0.626331 5_2:-55176494 None:intergenic
AAAACAGAACGGGACAGAGA+GGG 0.651600 5_2:+55174634 Msa0776700:five_prime_UTR
AGAGGAAAGGGGTAACCAAG+TGG 0.658019 5_2:+55174709 Msa0776700:five_prime_UTR
ACCGCACCCACTGAAACGGA+AGG 0.664522 5_2:-55174981 None:intergenic
TGGAAGAAGGAACTCGTCGG+CGG 0.695763 5_2:+55174729 Msa0776700:five_prime_UTR
ACATTACCAGAAACACCAGA+AGG 0.703448 5_2:-55174822 None:intergenic
AAGTGGAAGAAGGAACTCGT+CGG 0.709064 5_2:+55174726 Msa0776700:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTATGTTTTTGTTTGTT+TGG + chr5_2:55175614-55175633 Msa0776700:intron 15.0%
!!! TATAAATCTGAAATTTATTG+TGG + chr5_2:55175910-55175929 Msa0776700:five_prime_UTR 15.0%
!!! AATAATGCTTTTATTTTTTC+AGG + chr5_2:55176622-55176641 Msa0776700:intron 15.0%
!! ACAATATTCTTATAAATCAA+TGG + chr5_2:55177013-55177032 Msa0776700:intron 15.0%
!! TTTGTGTTAAACATTATGAA+GGG + chr5_2:55175278-55175297 Msa0776700:intron 20.0%
!! AAACTAACTAAACAAAAATC+AGG - chr5_2:55175970-55175989 None:intergenic 20.0%
!!! TTGTTTCAAAGAAAATTTTG+AGG + chr5_2:55176682-55176701 Msa0776700:intron 20.0%
!!! TGTTTCAAAGAAAATTTTGA+GGG + chr5_2:55176683-55176702 Msa0776700:intron 20.0%
! ACAAACAAAAATAAAACACC+AGG - chr5_2:55175236-55175255 None:intergenic 25.0%
! GTTTGTGTTAAACATTATGA+AGG + chr5_2:55175277-55175296 Msa0776700:intron 25.0%
! AGGAAAATTGGTTATTCTAA+TGG + chr5_2:55175405-55175424 Msa0776700:intron 25.0%
!! ATGGTTTAAAACCTTGAATA+TGG - chr5_2:55175644-55175663 None:intergenic 25.0%
!!! ATATTCAAGGTTTTAAACCA+TGG + chr5_2:55175643-55175662 Msa0776700:intron 25.0%
!! TAATTTTGTGAACAAATTGC+AGG + chr5_2:55175870-55175889 Msa0776700:intron 25.0%
!! AAATCTGAAATTTATTGTGG+TGG + chr5_2:55175913-55175932 Msa0776700:five_prime_UTR 25.0%
! TTGAATTGTTAATTGATGCT+TGG + chr5_2:55175988-55176007 Msa0776700:intron 25.0%
!!! CTTGGTATTTTTTATATACG+TGG + chr5_2:55176006-55176025 Msa0776700:intron 25.0%
! AGAAGCAAACATATTATTCT+TGG + chr5_2:55176044-55176063 Msa0776700:intron 25.0%
!! AATCATAAAACATGTTTTGC+AGG + chr5_2:55176423-55176442 Msa0776700:intron 25.0%
!! AGCTAGATTTTAAATCAAGT+AGG - chr5_2:55176538-55176557 None:intergenic 25.0%
! GAATTATGTGTTCTATTTGT+AGG + chr5_2:55176736-55176755 Msa0776700:intron 25.0%
! TTGTAAAGTAGTTTGATCTA+TGG + chr5_2:55176884-55176903 Msa0776700:intron 25.0%
! TATTCTTATAAATCAATGGC+AGG + chr5_2:55177017-55177036 Msa0776700:intron 25.0%
!! ATGACTCAAATTTTCTGATA+TGG + chr5_2:55177042-55177061 Msa0776700:intron 25.0%
! TGCTTTGTTGAAAATTCATC+AGG + chr5_2:55174918-55174937 Msa0776700:intron 30.0%
! CAGGTTTTTCACTGTATATA+CGG + chr5_2:55174937-55174956 Msa0776700:intron 30.0%
! AGGTTTTTCACTGTATATAC+GGG + chr5_2:55174938-55174957 Msa0776700:intron 30.0%
TGAAGAAAAACTGAAGAAGA+AGG - chr5_2:55175051-55175070 None:intergenic 30.0%
! TTTTCTTCATCATCGATCAA+AGG + chr5_2:55175062-55175081 Msa0776700:five_prime_UTR 30.0%
!!! GTGGGTTTTTGATTTTTGAT+CGG + chr5_2:55175084-55175103 Msa0776700:intron 30.0%
!! TGTTTGTTGTTAATCGATTG+AGG + chr5_2:55175178-55175197 Msa0776700:intron 30.0%
AACAAAAACCCAGTAACATT+AGG - chr5_2:55175260-55175279 None:intergenic 30.0%
! TGTTTGATGAATTGATTCAG+AGG + chr5_2:55175331-55175350 Msa0776700:intron 30.0%
! GTTTGATGAATTGATTCAGA+GGG + chr5_2:55175332-55175351 Msa0776700:intron 30.0%
AATTGTGTTTCAAATCCAAG+TGG + chr5_2:55175360-55175379 Msa0776700:intron 30.0%
! GGTTCAAATTCTAGGAAAAT+TGG + chr5_2:55175393-55175412 Msa0776700:intron 30.0%
TTCTAATGGTTCTTCTTCAA+CGG + chr5_2:55175419-55175438 Msa0776700:intron 30.0%
!! GACGAATTTTTCGAAGAATT+TGG + chr5_2:55175446-55175465 Msa0776700:intron 30.0%
!!! TAAAAGTGGTTTTGATAAGC+AGG + chr5_2:55175485-55175504 Msa0776700:intron 30.0%
AATGCTCTTGTTCTATATGA+TGG - chr5_2:55176295-55176314 None:intergenic 30.0%
!!! TTTTATTTTTTCAGGCAGCT+GGG + chr5_2:55176630-55176649 Msa0776700:intron 30.0%
TTACATGAAATGTTGCATGT+TGG + chr5_2:55176780-55176799 Msa0776700:intron 30.0%
TGATAGTAATCTCTCAGTAT+AGG + chr5_2:55176836-55176855 Msa0776700:intron 30.0%
ATATGCCAATCTCTTATGAA+AGG + chr5_2:55177074-55177093 Msa0776700:intron 30.0%
TTGAACCTTTCATAAGAGAT+TGG - chr5_2:55177082-55177101 None:intergenic 30.0%
! TTCAATTAGATAGAGTGCTT+TGG + chr5_2:55177097-55177116 Msa0776700:intron 30.0%
ATGTAACTGAAACCTTGATT+CGG + chr5_2:55177166-55177185 Msa0776700:three_prime_UTR 30.0%
! ATGCTTCAAAGGCTTTAATT+TGG + chr5_2:55177211-55177230 Msa0776700:three_prime_UTR 30.0%
! TGCTTCAAAGGCTTTAATTT+GGG + chr5_2:55177212-55177231 Msa0776700:three_prime_UTR 30.0%
GAAAGAATTCAGATTTAGCA+AGG + chr5_2:55177242-55177261 Msa0776700:three_prime_UTR 30.0%
ATTGCAGCGAATTGAAAGTT+TGG + chr5_2:55174786-55174805 Msa0776700:five_prime_UTR 35.0%
! TTTTTCACTGTATATACGGG+TGG + chr5_2:55174941-55174960 Msa0776700:five_prime_UTR 35.0%
GAAAAACTGAAGAAGAAGGT+GGG - chr5_2:55175047-55175066 None:intergenic 35.0%
AGAAAAACTGAAGAAGAAGG+TGG - chr5_2:55175048-55175067 None:intergenic 35.0%
CTCAATCGCACAAAAGAAAA+AGG - chr5_2:55175156-55175175 None:intergenic 35.0%
!! GTCGTTGCATGTTTTTGTTT+TGG + chr5_2:55175210-55175229 Msa0776700:intron 35.0%
TTGTTTGTGCCTAATGTTAC+TGG + chr5_2:55175248-55175267 Msa0776700:intron 35.0%
TGTTTGTGCCTAATGTTACT+GGG + chr5_2:55175249-55175268 Msa0776700:intron 35.0%
!! GTTCTACTGGTTCAAATTCT+AGG + chr5_2:55175385-55175404 Msa0776700:intron 35.0%
!! TTTTCGAAGAATTTGGCTTC+TGG + chr5_2:55175453-55175472 Msa0776700:intron 35.0%
!!! AAGAATTTGGCTTCTGGTTT+TGG + chr5_2:55175459-55175478 Msa0776700:intron 35.0%
!!! TCTGGTTTTGGTTCTAAAAG+TGG + chr5_2:55175471-55175490 Msa0776700:intron 35.0%
TGTAAACAATTCAGAACCCT+TGG - chr5_2:55175565-55175584 None:intergenic 35.0%
!! AAGGGTTCTGAATTGTTTAC+AGG + chr5_2:55175564-55175583 Msa0776700:intron 35.0%
TGTTTGGATAGCCATATTCA+AGG + chr5_2:55175630-55175649 Msa0776700:intron 35.0%
!!! TTCAAGGTTTTAAACCATGG+TGG + chr5_2:55175646-55175665 Msa0776700:intron 35.0%
! GTGGTAGTAATGATAACGAT+CGG + chr5_2:55175665-55175684 Msa0776700:intron 35.0%
CTTGATATCGTTGAGAATTG+CGG + chr5_2:55175701-55175720 Msa0776700:intron 35.0%
TCCATCAAAGTCTAATTCGA+CGG - chr5_2:55175757-55175776 None:intergenic 35.0%
!!! TGTTGTTTTGTAGGTCAAGA+TGG + chr5_2:55175794-55175813 Msa0776700:intron 35.0%
TTATTCTTGGAATCTCCTGT+TGG + chr5_2:55176057-55176076 Msa0776700:five_prime_UTR 35.0%
TAAGAAAATCCAACTCCAAC+AGG - chr5_2:55176075-55176094 None:intergenic 35.0%
TCTTAGTTGTGCATATTCAC+TGG - chr5_2:55176109-55176128 None:intergenic 35.0%
! CTTACACTTTTCTGCACAAT+TGG + chr5_2:55176257-55176276 Msa0776700:five_prime_UTR 35.0%
!!! ACAAGAGCATTTTACATAGC+AGG + chr5_2:55176304-55176323 Msa0776700:five_prime_UTR 35.0%
TGAGATCAATATGAAGGGAA+GGG - chr5_2:55176497-55176516 None:intergenic 35.0%
TTGAGATCAATATGAAGGGA+AGG - chr5_2:55176498-55176517 None:intergenic 35.0%
TTCCCTTCATATTGATCTCA+AGG + chr5_2:55176497-55176516 Msa0776700:CDS 35.0%
TCCCTTCATATTGATCTCAA+GGG + chr5_2:55176498-55176517 Msa0776700:CDS 35.0%
!!! TATTGATCTCAAGGGCATTT+TGG + chr5_2:55176506-55176525 Msa0776700:CDS 35.0%
!!! CTTTTATTTTTTCAGGCAGC+TGG + chr5_2:55176629-55176648 Msa0776700:intron 35.0%
AGCAGTTTCAAAGTTGTGTT+TGG + chr5_2:55176803-55176822 Msa0776700:intron 35.0%
TCTCTGTCTCATATAGTGTT+TGG + chr5_2:55176983-55177002 Msa0776700:intron 35.0%
! AATTAGATAGAGTGCTTTGG+TGG + chr5_2:55177100-55177119 Msa0776700:intron 35.0%
! ATTAGATAGAGTGCTTTGGT+GGG + chr5_2:55177101-55177120 Msa0776700:intron 35.0%
TACTTGCAGAATGTTCATCA+AGG + chr5_2:55177141-55177160 Msa0776700:intron 35.0%
AGAGGAGAAGAAGGAAAAGA+AGG + chr5_2:55174664-55174683 Msa0776700:five_prime_UTR 40.0%
GAGGAGAAGAAGGAAAAGAA+GGG + chr5_2:55174665-55174684 Msa0776700:five_prime_UTR 40.0%
! TGAAAGTTTGGTTCCCAAAG+TGG + chr5_2:55174798-55174817 Msa0776700:five_prime_UTR 40.0%
ACATTACCAGAAACACCAGA+AGG - chr5_2:55174825-55174844 None:intergenic 40.0%
TCTTCATCATCGATCAAAGG+TGG + chr5_2:55175065-55175084 Msa0776700:intron 40.0%
CTTCATCATCGATCAAAGGT+GGG + chr5_2:55175066-55175085 Msa0776700:intron 40.0%
!!! TGCATGTTTTTGTTTTGGCC+TGG + chr5_2:55175215-55175234 Msa0776700:intron 40.0%
TGTGTTTCAAATCCAAGTGG+TGG + chr5_2:55175363-55175382 Msa0776700:intron 40.0%
AAGCAGGTTTCTTGAAGTGT+TGG + chr5_2:55175501-55175520 Msa0776700:intron 40.0%
!! AAGTGTTGGTTCTTCTACCT+TGG + chr5_2:55175515-55175534 Msa0776700:intron 40.0%
ATCTTTCTCAACACGAACCA+AGG - chr5_2:55175535-55175554 None:intergenic 40.0%
! TGAGAAAGATGTTTCTGCCA+AGG + chr5_2:55175545-55175564 Msa0776700:intron 40.0%
! GAGAAAGATGTTTCTGCCAA+GGG + chr5_2:55175546-55175565 Msa0776700:intron 40.0%
CGTTATCATTACTACCACCA+TGG - chr5_2:55175663-55175682 None:intergenic 40.0%
GTCTAATTCGACGGCAATTT+AGG - chr5_2:55175748-55175767 None:intergenic 40.0%
TTGATGGAATGTTGTGCTGA+TGG + chr5_2:55175769-55175788 Msa0776700:intron 40.0%
! TGATGGAATGTTGTGCTGAT+GGG + chr5_2:55175770-55175789 Msa0776700:intron 40.0%
!!! CTGATGGGATGTTGTTTTGT+AGG + chr5_2:55175785-55175804 Msa0776700:intron 40.0%
TTATTGTGGTGGTCTTTGAG+AGG + chr5_2:55175924-55175943 Msa0776700:intron 40.0%
GCCCTTGAGATCAATATGAA+GGG - chr5_2:55176502-55176521 None:intergenic 40.0%
TGCCCTTGAGATCAATATGA+AGG - chr5_2:55176503-55176522 None:intergenic 40.0%
TCTCATGTTGCAAGTGATAG+AGG + chr5_2:55176708-55176727 Msa0776700:intron 40.0%
TGCTTTCAGAAACCGAATCA+AGG - chr5_2:55177181-55177200 None:intergenic 40.0%
!! TGATTCGGTTTCTGAAAGCA+AGG + chr5_2:55177181-55177200 Msa0776700:three_prime_UTR 40.0%
! GATTCGGTTTCTGAAAGCAA+GGG + chr5_2:55177182-55177201 Msa0776700:three_prime_UTR 40.0%
AAGGGAATGGAATGCTTCAA+AGG + chr5_2:55177200-55177219 Msa0776700:three_prime_UTR 40.0%
AAAACAGAACGGGACAGAGA+GGG + chr5_2:55174634-55174653 Msa0776700:five_prime_UTR 45.0%
AAGGAAAAGAAGGGAGATCG+AGG + chr5_2:55174674-55174693 Msa0776700:five_prime_UTR 45.0%
AGGAAAAGAAGGGAGATCGA+GGG + chr5_2:55174675-55174694 Msa0776700:five_prime_UTR 45.0%
GAGAGGAGAAGAAGAGGAAA+GGG + chr5_2:55174697-55174716 Msa0776700:five_prime_UTR 45.0%
AGAGGAGAAGAAGAGGAAAG+GGG + chr5_2:55174698-55174717 Msa0776700:five_prime_UTR 45.0%
AAGTGGAAGAAGGAACTCGT+CGG + chr5_2:55174726-55174745 Msa0776700:five_prime_UTR 45.0%
ATTGGAAGATTCGGCGATTC+CGG + chr5_2:55174756-55174775 Msa0776700:five_prime_UTR 45.0%
AACACCAGAAGGACCACTTT+GGG - chr5_2:55174814-55174833 None:intergenic 45.0%
AAACACCAGAAGGACCACTT+TGG - chr5_2:55174815-55174834 None:intergenic 45.0%
TGAGAAACCTTCCGTTTCAG+TGG + chr5_2:55174974-55174993 Msa0776700:five_prime_UTR 45.0%
GAGAAACCTTCCGTTTCAGT+GGG + chr5_2:55174975-55174994 Msa0776700:five_prime_UTR 45.0%
! AATCCAAGTGGTGGTTCTAC+TGG + chr5_2:55175372-55175391 Msa0776700:intron 45.0%
GCCGTCGAATTAGACTTTGA+TGG + chr5_2:55175753-55175772 Msa0776700:intron 45.0%
TTGGAATCTCCTGTTGGAGT+TGG + chr5_2:55176063-55176082 Msa0776700:five_prime_UTR 45.0%
ATGATGAGTTCAGCAAGCTC+TGG - chr5_2:55176459-55176478 None:intergenic 45.0%
! GGTTTCTGAAAGCAAGGGAA+TGG + chr5_2:55177187-55177206 Msa0776700:three_prime_UTR 45.0%
CAAAACAGAACGGGACAGAG+AGG + chr5_2:55174633-55174652 Msa0776700:five_prime_UTR 50.0%
GGAGAGGAGAAGAAGAGGAA+AGG + chr5_2:55174696-55174715 Msa0776700:five_prime_UTR 50.0%
AGAGGAAAGGGGTAACCAAG+TGG + chr5_2:55174709-55174728 Msa0776700:five_prime_UTR 50.0%
AGGGGTAACCAAGTGGAAGA+AGG + chr5_2:55174716-55174735 Msa0776700:five_prime_UTR 50.0%
GACGAGTTCCTTCTTCCACT+TGG - chr5_2:55174727-55174746 None:intergenic 50.0%
ATTCGCTGCAATTTCGTCGC+CGG - chr5_2:55174778-55174797 None:intergenic 50.0%
AGTGGTCCTTCTGGTGTTTC+TGG + chr5_2:55174816-55174835 Msa0776700:five_prime_UTR 50.0%
GAACCAGTAGAACCACCACT+TGG - chr5_2:55175378-55175397 None:intergenic 50.0%
ATAGCAGGAGAGAGCTATGC+AGG + chr5_2:55176319-55176338 Msa0776700:exon 50.0%
CCAATCCAGCTCACTCTTCA+GGG - chr5_2:55176659-55176678 None:intergenic 50.0%
AAGAAGGGAGATCGAGGGAG+AGG + chr5_2:55174680-55174699 Msa0776700:five_prime_UTR 55.0%
TCGAGGGAGAGGAGAAGAAG+AGG + chr5_2:55174657-55174676 Msa0776700:five_prime_UTR 55.0%
TGGAAGAAGGAACTCGTCGG+CGG + chr5_2:55174729-55174748 Msa0776700:five_prime_UTR 55.0%
GGCGGTGGGATTGGAAGATT+CGG + chr5_2:55174747-55174766 Msa0776700:five_prime_UTR 55.0%
GGTTCCCAAAGTGGTCCTTC+TGG + chr5_2:55174807-55174826 Msa0776700:five_prime_UTR 55.0%
ACCTTCCGTTTCAGTGGGTG+CGG + chr5_2:55174980-55174999 Msa0776700:five_prime_UTR 55.0%
GGAAACCCTGAAGAGTGAGC+TGG + chr5_2:55176651-55176670 Msa0776700:intron 55.0%
CCCTGAAGAGTGAGCTGGAT+TGG + chr5_2:55176656-55176675 Msa0776700:intron 55.0%
GCCAATCCAGCTCACTCTTC+AGG - chr5_2:55176660-55176679 None:intergenic 55.0%
AACGGGACAGAGAGGGTCGA+GGG + chr5_2:55174641-55174660 Msa0776700:five_prime_UTR 60.0%
GGTCGAGGGAGAGGAGAAGA+AGG + chr5_2:55174655-55174674 Msa0776700:five_prime_UTR 60.0%
AAGAAGGAACTCGTCGGCGG+TGG + chr5_2:55174732-55174751 Msa0776700:five_prime_UTR 60.0%
AGAAGGAACTCGTCGGCGGT+GGG + chr5_2:55174733-55174752 Msa0776700:five_prime_UTR 60.0%
ACCGCACCCACTGAAACGGA+AGG - chr5_2:55174984-55175003 None:intergenic 60.0%
CGCAACCGCACCCACTGAAA+CGG - chr5_2:55174988-55175007 None:intergenic 60.0%
CGTTTCAGTGGGTGCGGTTG+CGG + chr5_2:55174986-55175005 Msa0776700:five_prime_UTR 60.0%
GTTTCAGTGGGTGCGGTTGC+GGG + chr5_2:55174987-55175006 Msa0776700:five_prime_UTR 60.0%
GAACGGGACAGAGAGGGTCG+AGG + chr5_2:55174640-55174659 Msa0776700:five_prime_UTR 65.0%
GACAGAGAGGGTCGAGGGAG+AGG + chr5_2:55174646-55174665 Msa0776700:five_prime_UTR 65.0%
GAACTCGTCGGCGGTGGGAT+TGG + chr5_2:55174738-55174757 Msa0776700:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 55174632 55177281 55174632 ID=Msa0776700;Name=Msa0776700
chr5_2 mRNA 55174632 55177281 55174632 ID=Msa0776700-mRNA-1;Parent=Msa0776700;Name=Msa0776700-mRNA-1;_AED=0.33;_eAED=0.33;_QI=576|0.16|0.28|0.57|0.83|1|7|131|29
chr5_2 exon 55174632 55174837 55174632 ID=Msa0776700-mRNA-1:exon:16344;Parent=Msa0776700-mRNA-1
chr5_2 exon 55174940 55175083 55174940 ID=Msa0776700-mRNA-1:exon:16345;Parent=Msa0776700-mRNA-1
chr5_2 exon 55175892 55175934 55175892 ID=Msa0776700-mRNA-1:exon:16346;Parent=Msa0776700-mRNA-1
chr5_2 exon 55176046 55176141 55176046 ID=Msa0776700-mRNA-1:exon:16347;Parent=Msa0776700-mRNA-1
chr5_2 exon 55176248 55176340 55176248 ID=Msa0776700-mRNA-1:exon:16348;Parent=Msa0776700-mRNA-1
chr5_2 exon 55176445 55176527 55176445 ID=Msa0776700-mRNA-1:exon:16349;Parent=Msa0776700-mRNA-1
chr5_2 exon 55177150 55177281 55177150 ID=Msa0776700-mRNA-1:exon:16350;Parent=Msa0776700-mRNA-1
chr5_2 five_prime_UTR 55174632 55174837 55174632 ID=Msa0776700-mRNA-1:five_prime_utr;Parent=Msa0776700-mRNA-1
chr5_2 five_prime_UTR 55174940 55175083 55174940 ID=Msa0776700-mRNA-1:five_prime_utr;Parent=Msa0776700-mRNA-1
chr5_2 five_prime_UTR 55175892 55175934 55175892 ID=Msa0776700-mRNA-1:five_prime_utr;Parent=Msa0776700-mRNA-1
chr5_2 five_prime_UTR 55176046 55176141 55176046 ID=Msa0776700-mRNA-1:five_prime_utr;Parent=Msa0776700-mRNA-1
chr5_2 five_prime_UTR 55176248 55176334 55176248 ID=Msa0776700-mRNA-1:five_prime_utr;Parent=Msa0776700-mRNA-1
chr5_2 CDS 55176335 55176340 55176335 ID=Msa0776700-mRNA-1:cds;Parent=Msa0776700-mRNA-1
chr5_2 CDS 55176445 55176527 55176445 ID=Msa0776700-mRNA-1:cds;Parent=Msa0776700-mRNA-1
chr5_2 CDS 55177150 55177150 55177150 ID=Msa0776700-mRNA-1:cds;Parent=Msa0776700-mRNA-1
chr5_2 three_prime_UTR 55177151 55177281 55177151 ID=Msa0776700-mRNA-1:three_prime_utr;Parent=Msa0776700-mRNA-1
Gene Sequence

>Msa0776700

ATGCAGGAAAGTATGTACCAGAGCTTGCTGAACTCATCATTGATAGAAACAATAACCCTTCCCTTCATATTGATCTCAAGGGCATTTTGA
Protein sequence

>Msa0776700

MQESMYQSLLNSSLIETITLPFILISRAF*