AlfalfaGEDB | Msa0777410

M. sativa cultivar zhongmu-4 / Msa0777410

KEGG: K07198|K12761
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009268 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0777410	sp\|O22932\|CIPKB_ARATH	56.229	297	116	7	35	321	140	432	5.60e-108	325

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0777410	G7K0R1	94.000	300	18	0	35	334	141	440	0.0	553

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0777410	Msa0814410	0.807685	4.480438e-50	-8.615850e-47
Msa0777410	Msa0854920	0.803391	3.564672e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0777410	MtrunA17_Chr5g0432491	94.000	300	18	0	35	334	141	440	0.0	553
Msa0777410	MtrunA17_Chr5g0432491	78.571	56	9	1	1	56	1	53	4.12e-14	72.8
Msa0777410	MtrunA17_Chr3g0107051	60.265	302	105	5	35	326	138	434	7.79e-126	367
Msa0777410	MtrunA17_Chr2g0284031	45.342	322	134	5	35	317	148	466	5.29e-90	278
Msa0777410	MtrunA17_Chr7g0244561	47.651	298	139	5	35	326	135	421	8.41e-90	275
Msa0777410	MtrunA17_Chr8g0379091	42.609	345	144	8	35	330	143	482	9.54e-87	270
Msa0777410	MtrunA17_Chr2g0284011	44.262	305	136	9	50	325	144	443	4.94e-76	240
Msa0777410	MtrunA17_Chr5g0441011	44.558	294	142	7	52	327	148	438	1.62e-74	236
Msa0777410	MtrunA17_Chr7g0244521	40.850	306	154	7	35	315	129	432	2.86e-69	223
Msa0777410	MtrunA17_Chr4g0013131	43.289	298	146	8	50	326	144	439	3.05e-69	223

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0777410	AT2G30360.1	56.229	297	116	7	35	321	140	432	5.69e-109	325
Msa0777410	AT4G18700.1	47.649	319	132	4	35	320	143	459	1.32e-95	292
Msa0777410	AT5G45810.1	44.048	336	145	6	37	331	143	476	2.75e-88	273
Msa0777410	AT5G01820.1	49.508	305	147	4	35	333	139	442	5.87e-87	268
Msa0777410	AT1G29230.1	48.562	313	127	5	33	313	189	499	9.90e-87	270
Msa0777410	AT2G34180.1	44.373	311	136	4	35	313	174	479	1.06e-84	265
Msa0777410	AT5G45820.1	41.379	290	161	4	35	315	129	418	1.22e-78	247
Msa0777410	AT4G30960.1	44.108	297	140	7	40	315	142	433	3.98e-72	230
Msa0777410	AT1G30270.3	43.011	279	136	5	51	307	68	345	4.45e-72	228
Msa0777410	AT1G30270.1	43.011	279	136	5	51	307	165	442	5.34e-71	228

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Find 116 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGCAAGTTCGAATTGA+CGG	0.485186	5_2:+56353281	Msa0777410:CDS
AACACCTGCATATGTTGCAC+CGG	0.597649	5_2:+56353325	Msa0777410:CDS
AAGAGCAAGTTCGAATTGAC+GGG	0.560110	5_2:+56353282	Msa0777410:CDS
AAGGTGAGAGGTTTATGTTG+AGG	0.581675	5_2:+56353771	Msa0777410:CDS
AAGGTGGATATTTGGTCATG+TGG	0.602935	5_2:+56353380	Msa0777410:CDS
AAGTGTGGAAGAATAAGTTG+AGG	0.714623	5_2:+56353996	Msa0777410:CDS
AATTTGAAGGTTTCGGATTT+TGG	0.174809	5_2:+56353245	Msa0777410:CDS
ACAGGCAGCAGCGCCAGCTC+CGG	0.420125	5_2:+56352640	Msa0777410:CDS
ACCACCACCACCGCACTCTT+CGG	0.497587	5_2:+56352674	Msa0777410:CDS
ACCGGAGAAGGTTGTGGAGA+AGG	0.502457	5_2:+56353799	Msa0777410:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTATACATCACCATCAAATT+TGG	-	chr5_2:56353452-56353471	None:intergenic	30.0%
TGTATAGGAAGATATATAGC+GGG	+	chr5_2:56353465-56353484	Msa0777410:CDS	30.0%
TGAGAAGAAGGTTAATAATG+GGG	+	chr5_2:56353652-56353671	Msa0777410:CDS	30.0%
CTATACATCACCATCAAATT+TGG	-	chr5_2:56353452-56353471	None:intergenic	30.0%
TGTATAGGAAGATATATAGC+GGG	+	chr5_2:56353465-56353484	Msa0777410:CDS	30.0%
TGAGAAGAAGGTTAATAATG+GGG	+	chr5_2:56353652-56353671	Msa0777410:CDS	30.0%
AAGAAAAAAATCACCGCAAC+AGG	+	chr5_2:56352800-56352819	Msa0777410:intron	35.0%
TTCGTCATCCGAATATTGTT+CGG	+	chr5_2:56352867-56352886	Msa0777410:intron	35.0%
TCATGAAGCCGAACAATATT+CGG	-	chr5_2:56352878-56352897	None:intergenic	35.0%
TTCGCCAAAATTGCGAATAA+AGG	+	chr5_2:56352959-56352978	Msa0777410:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	56352626	56354323	56352626	ID=Msa0777410;Name=Msa0777410
chr5_2	mRNA	56352626	56354323	56352626	ID=Msa0777410-mRNA-1;Parent=Msa0777410;Name=Msa0777410-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|0\|0.5\|1\|0\|0.5\|2\|230\|334
chr5_2	exon	56352626	56352778	56352626	ID=Msa0777410-mRNA-1:exon:16665;Parent=Msa0777410-mRNA-1
chr5_2	exon	56353242	56354323	56353242	ID=Msa0777410-mRNA-1:exon:16666;Parent=Msa0777410-mRNA-1
chr5_2	CDS	56352626	56352778	56352626	ID=Msa0777410-mRNA-1:cds;Parent=Msa0777410-mRNA-1
chr5_2	CDS	56353242	56354093	56353242	ID=Msa0777410-mRNA-1:cds;Parent=Msa0777410-mRNA-1
chr5_2	three_prime_UTR	56354094	56354323	56354094	ID=Msa0777410-mRNA-1:three_prime_utr;Parent=Msa0777410-mRNA-1

Gene Sequence

>Msa0777410

ATGTCGGATATCGAACAGGCAGCAGCGCCAGCTCCGGCAGATGCCTCCACCACCACCACCGCACTCTTCGGCAAATACGAGCTCGGCAAACTCTTAGGATGCGGCGCGTTTGCGAAAGTATACCACGCGCGGAACACGGAGAATGGACAGAGCGGGAATTTGAAGGTTTCGGATTTTGGTTTGAGTGCAGTGAAAGAGCAAGTTCGAATTGACGGGATGTTACACACGCTTTGTGGAACACCTGCATATGTTGCACCGGAGATTTTAGCGAAGAGAGGTTATGATGGAGCTAAGGTGGATATTTGGTCATGTGGTATTATCTTGTTTGTTCTTGTGGCTGGTTATCTTCCATTTAATGATCCAAATTTGATGGTGATGTATAGGAAGATATATAGCGGGGAATTTAAGTGTCCACGATGGGTTTCACCGGATCTAAAGCGGTTTTTATCGCGGTTGATGGATACGAATCCGGAGACGAGGATAACTGTTGATGAGATTTTGAGGGATCCTTGGTTTAGAAAAGGGTATAAAGAGGTGAAGTTTTATGAAGAGGGTTTTGAGTTTGAGAAGAAGGTTAATAATGGGGAAGAAGAGGGAAAACCGTTGGATTTCAATGCGTTTGATATTATATCGTTTTTCTCCAGAGGGTTGAATCTTTCTGGGTTTTTTATTGATGGTGGTGAAGGTGAGAGGTTTATGTTGAGGGAGTTACCGGAGAAGGTTGTGGAGAAGGCTGAGGCAGCCGCGAAGGCAGAGGGGCTTGTGGTGAGGAGGAAGAAGGAATGTGGGGTGGAAATTGAAGAGGAGAATGGTGATTTGGTGATTGGGATTGAGATTTACCGATTAACCGCTGAGATGGTTGTGGTTGAGGTGAAGAGGTTTGGTGGGGATGCGGTTGCTTTCGAGGAAGTGTGGAAGAATAAGTTGAGGCCACATTTGTGCGATGCAACGACGTCGAACCAGGACCAACCTCAGTCTCATGCGGTCCCTGCTAGTGCTGACTAG

Protein sequence

>Msa0777410

MSDIEQAAAPAPADASTTTTALFGKYELGKLLGCGAFAKVYHARNTENGQSGNLKVSDFGLSAVKEQVRIDGMLHTLCGTPAYVAPEILAKRGYDGAKVDIWSCGIILFVLVAGYLPFNDPNLMVMYRKIYSGEFKCPRWVSPDLKRFLSRLMDTNPETRITVDEILRDPWFRKGYKEVKFYEEGFEFEKKVNNGEEEGKPLDFNAFDIISFFSRGLNLSGFFIDGGEGERFMLRELPEKVVEKAEAAAKAEGLVVRRKKECGVEIEEENGDLVIGIEIYRLTAEMVVVEVKRFGGDAVAFEEVWKNKLRPHLCDATTSNQDQPQSHAVPASAD*