Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0778220 | sp|Q944A7|BSK1_ARATH | 80.744 | 457 | 42 | 3 | 59 | 470 | 57 | 512 | 0.0 | 752 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0778220 | Msa0826670 | 0.837206 | 5.958294e-57 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0778220 | MtrunA17_Chr5g0448561 | 88.760 | 516 | 10 | 4 | 1 | 470 | 11 | 524 | 0.0 | 930 |
| Msa0778220 | MtrunA17_Chr8g0349041 | 58.511 | 470 | 140 | 6 | 39 | 462 | 23 | 483 | 0.0 | 537 |
| Msa0778220 | MtrunA17_Chr4g0054861 | 56.455 | 457 | 151 | 5 | 59 | 469 | 44 | 498 | 0.0 | 530 |
| Msa0778220 | MtrunA17_Chr4g0032361 | 54.979 | 482 | 164 | 6 | 29 | 464 | 16 | 490 | 0.0 | 526 |
| Msa0778220 | MtrunA17_Chr7g0247441 | 56.263 | 471 | 152 | 5 | 39 | 463 | 23 | 485 | 0.0 | 524 |
| Msa0778220 | MtrunA17_Chr8g0386761 | 55.626 | 471 | 156 | 5 | 39 | 462 | 21 | 485 | 0.0 | 518 |
| Msa0778220 | MtrunA17_Chr4g0008911 | 65.642 | 358 | 111 | 5 | 59 | 409 | 40 | 392 | 3.70e-166 | 474 |
| Msa0778220 | MtrunA17_Chr2g0328731 | 48.966 | 290 | 92 | 5 | 97 | 386 | 8 | 241 | 7.34e-85 | 261 |
| Msa0778220 | MtrunA17_Chr7g0251771 | 34.185 | 313 | 181 | 9 | 63 | 350 | 39 | 351 | 4.36e-46 | 164 |
| Msa0778220 | MtrunA17_Chr7g0237401 | 32.941 | 340 | 197 | 7 | 41 | 349 | 59 | 398 | 4.46e-46 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0778220 | AT4G35230.1 | 80.744 | 457 | 42 | 3 | 59 | 470 | 57 | 512 | 0.0 | 752 |
| Msa0778220 | AT5G59010.1 | 57.261 | 482 | 148 | 6 | 29 | 464 | 16 | 485 | 0.0 | 556 |
| Msa0778220 | AT1G50990.1 | 58.093 | 451 | 140 | 4 | 60 | 463 | 57 | 505 | 0.0 | 533 |
| Msa0778220 | AT4G00710.1 | 57.974 | 464 | 142 | 6 | 45 | 462 | 25 | 481 | 0.0 | 522 |
| Msa0778220 | AT4G00710.2 | 57.974 | 464 | 142 | 6 | 45 | 462 | 25 | 481 | 0.0 | 522 |
| Msa0778220 | AT1G50990.2 | 57.650 | 451 | 137 | 5 | 60 | 463 | 57 | 500 | 0.0 | 522 |
| Msa0778220 | AT3G54030.1 | 57.650 | 451 | 144 | 3 | 59 | 463 | 37 | 486 | 0.0 | 521 |
| Msa0778220 | AT1G63500.1 | 57.301 | 452 | 141 | 4 | 60 | 465 | 41 | 486 | 0.0 | 521 |
| Msa0778220 | AT5G41260.1 | 55.224 | 469 | 158 | 4 | 41 | 463 | 22 | 484 | 0.0 | 518 |
| Msa0778220 | AT5G46570.1 | 55.824 | 455 | 151 | 6 | 60 | 468 | 38 | 488 | 9.26e-180 | 512 |
Find 143 sgRNAs with CRISPR-Local
Find 684 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCAAAACAAGAGAGACG+CGG | 0.753060 | 5_2:+58626805 | None:intergenic |
| AAAGTGAAAGATGAAGGATC+TGG | 0.612826 | 5_2:+58627489 | None:intergenic |
| AAGAAACTTACATGACTGGG+AGG | 0.670115 | 5_2:+58626541 | None:intergenic |
| AAGAGCAACTCTTAAACGCA+TGG | 0.607414 | 5_2:+58627032 | None:intergenic |
| AAGAGGCAAAGAGGAGCGAG+AGG | 0.573461 | 5_2:-58619557 | Msa0778220:CDS |
| AAGCTGTAAATGACACTTTC+AGG | 0.194968 | 5_2:+58626604 | None:intergenic |
| AATAACCTCAATCTGACACC+AGG | 0.671328 | 5_2:+58619254 | None:intergenic |
| AATATTAATAATGAAAGACT+TGG | 0.550075 | 5_2:+58619131 | None:intergenic |
| AATTATTGGTAGCTGCTTTG+AGG | 0.445705 | 5_2:+58627445 | None:intergenic |
| ACAATAAATTTCATCAACAG+TGG | 0.602997 | 5_2:+58619302 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAGAATAATCACAAGAAGA+AGG | - | chr5_2:58618948-58618967 | Msa0778220:three_prime_UTR | 30.0% | |
| TGATGATTCTTTGTCTTTGT+CGG | + | chr5_2:58619058-58619077 | None:intergenic | 30.0% | |
| TCTGAAAAAGTGAAAGATGA+AGG | + | chr5_2:58619090-58619109 | None:intergenic | 30.0% | |
| CTTCCTTTGTAAACTAAGTT+AGG | + | chr5_2:58619192-58619211 | None:intergenic | 30.0% | |
| CTAACTTAGTTTACAAAGGA+AGG | - | chr5_2:58619190-58619209 | Msa0778220:three_prime_UTR | 30.0% | |
| ATAGCATATTACAGTGAAAG+AGG | + | chr5_2:58619416-58619435 | None:intergenic | 30.0% | |
| GGAAAATCAAACTATTGAGT+GGG | - | chr5_2:58619515-58619534 | Msa0778220:three_prime_UTR | 30.0% | |
| TTTGCATTATTGTACTTCTG+AGG | - | chr5_2:58619575-58619594 | Msa0778220:CDS | 30.0% | |
| TTGCATTATTGTACTTCTGA+GGG | - | chr5_2:58619576-58619595 | Msa0778220:CDS | 30.0% | |
| GCATTCAAATCATGGTATAA+AGG | + | chr5_2:58619606-58619625 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 58618925 | 58627667 | 58618925 | ID=Msa0778220;Name=Msa0778220 |
| chr5_2 | mRNA | 58618925 | 58627667 | 58618925 | ID=Msa0778220-mRNA-1;Parent=Msa0778220;Name=Msa0778220-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0.71|0.75|1|0.85|0.75|8|625|470 |
| chr5_2 | exon | 58618925 | 58619804 | 58618925 | ID=Msa0778220-mRNA-1:exon:17243;Parent=Msa0778220-mRNA-1 |
| chr5_2 | exon | 58620095 | 58620259 | 58620095 | ID=Msa0778220-mRNA-1:exon:17242;Parent=Msa0778220-mRNA-1 |
| chr5_2 | exon | 58625074 | 58625262 | 58625074 | ID=Msa0778220-mRNA-1:exon:17241;Parent=Msa0778220-mRNA-1 |
| chr5_2 | exon | 58626552 | 58626637 | 58626552 | ID=Msa0778220-mRNA-1:exon:17240;Parent=Msa0778220-mRNA-1 |
| chr5_2 | exon | 58626731 | 58626836 | 58626731 | ID=Msa0778220-mRNA-1:exon:17239;Parent=Msa0778220-mRNA-1 |
| chr5_2 | exon | 58626945 | 58627078 | 58626945 | ID=Msa0778220-mRNA-1:exon:17238;Parent=Msa0778220-mRNA-1 |
| chr5_2 | exon | 58627169 | 58627406 | 58627169 | ID=Msa0778220-mRNA-1:exon:17237;Parent=Msa0778220-mRNA-1 |
| chr5_2 | exon | 58627428 | 58627667 | 58627428 | ID=Msa0778220-mRNA-1:exon:17236;Parent=Msa0778220-mRNA-1 |
| chr5_2 | CDS | 58627428 | 58627667 | 58627428 | ID=Msa0778220-mRNA-1:cds;Parent=Msa0778220-mRNA-1 |
| chr5_2 | CDS | 58627169 | 58627406 | 58627169 | ID=Msa0778220-mRNA-1:cds;Parent=Msa0778220-mRNA-1 |
| chr5_2 | CDS | 58626945 | 58627078 | 58626945 | ID=Msa0778220-mRNA-1:cds;Parent=Msa0778220-mRNA-1 |
| chr5_2 | CDS | 58626731 | 58626836 | 58626731 | ID=Msa0778220-mRNA-1:cds;Parent=Msa0778220-mRNA-1 |
| chr5_2 | CDS | 58626552 | 58626637 | 58626552 | ID=Msa0778220-mRNA-1:cds;Parent=Msa0778220-mRNA-1 |
| chr5_2 | CDS | 58625074 | 58625262 | 58625074 | ID=Msa0778220-mRNA-1:cds;Parent=Msa0778220-mRNA-1 |
| chr5_2 | CDS | 58620095 | 58620259 | 58620095 | ID=Msa0778220-mRNA-1:cds;Parent=Msa0778220-mRNA-1 |
| chr5_2 | CDS | 58619550 | 58619804 | 58619550 | ID=Msa0778220-mRNA-1:cds;Parent=Msa0778220-mRNA-1 |
| chr5_2 | three_prime_UTR | 58618925 | 58619549 | 58618925 | ID=Msa0778220-mRNA-1:three_prime_utr;Parent=Msa0778220-mRNA-1 |
| Gene Sequence |
>Msa0778220 AGAGGAAGAGGAAGAGGAGGAAGTGAGAATAATCACAAGAAGAAGGAACAACAACAACAACAACAACAACCCTATCAACTTCAACACCTGAGGAGTAGCAACAATTTGCTGAATCAAACACCAAATGAAACCGACAAAGACAAAGAATCATCATCACCAGATCCTTCATCTTTCACTTTTTCAGAGTTTTCACTTTCAGACCTCAAAGCAGCTACCAATAATTTCAGCTCAGAATATATAGCACCTAACTTAGTTTACAAAGGAAGGTTACAGAATCAGAAACGTTGGATTGCTGTTAAGAAGTTCGGGAAAGCTGCTTGGCCGGATCCAAAACAGTTTGTTGAAGAAGCTTCTGGTGTTGGCAAATTGCGTCATCCTCGACTTGCTAATTTGATTGGTTATTGTTGTGATGGTGATGAGAGATTACTTGTTGCTGAGTTTATGCCTAATGATACCCTTGCTAAACACCTCTTTCACTGGGAAAATCAAACTATTGAGTGGGCCATGCGTTTAAGAGTTGCTCTTTATATTGCACAGGCTTTGCATTATTGTACTTCTGAGGGACGCCCTTTATACCATGATTTGAATGCTTATCGCGTTCTCTTCGATGAGGAAGGTGATCCGCGTCTCTCTTGTTTTGGTTTAATGAAGAACAGCAGGGATGGGAAAAGTTATAGCACAAACCTTGCTTACACACCTCCTGAATATTTAAGAAATGGAAGGGTTACCCCTGAAAGTGTCATTTACAGCTTTGGAACTGTTCTTCTTGACCTACTCAGTGGCAAGCACATCCCTCCCAGTCATGCACTTGATATGATACAGGGAAAAAACAACATGCTCTTGATGGATTCTCATTTGGAGGGGAAGTTTTCTACCGAGGAGGCAACAGTAGTTGTCAATCTCGCCTCTAAATGTTTGCAATATGAACCGCGAGAGCGGCCTAATACAAAGGATTTGGTCACAACACTTGCTCCGTTGCATACAAAACCTGATGTTCGTTCTCACATCATGCTTGGAATTCCAAAGCAAGAGGAAGCTCCATCAACCCCACAGCGCCCTCTTTCAGCAATGGGTGAGGCCTGCTCAAGAATGGACCTCACTGCAATACATCAGATCTTGGTTGCAACCCACTACAGAGATGACGAAGGGACTAATGAGTTCATTGATGTGGGAACGATGGTCTCACCTACAGTTTTTGCAAGGCGTAGCCTATGCTATCTGTTATGTGATCAACCAGATCCTGCTCTACGAGATGCAATGCAAGCACAATGTGTTTATCCAGACTGGCCCACATCTTTCTACATGCAGTCAGTTGCTCTTGCAAAGCTGAACATGCACAAGGATGCAGCTGACATGTTAAATGAGGCAGCTGCATTGGAAGAGAAGAGGCAAAGAGGAGCGAGAGGATCATAG |
| Protein sequence |
>Msa0778220 RGRGRGGSENNHKKKEQQQQQQQPYQLQHLRSSNNLLNQTPNETDKDKESSSPDPSSFTFSEFSLSDLKAATNNFSSEYIAPNLVYKGRLQNQKRWIAVKKFGKAAWPDPKQFVEEASGVGKLRHPRLANLIGYCCDGDERLLVAEFMPNDTLAKHLFHWENQTIEWAMRLRVALYIAQALHYCTSEGRPLYHDLNAYRVLFDEEGDPRLSCFGLMKNSRDGKSYSTNLAYTPPEYLRNGRVTPESVIYSFGTVLLDLLSGKHIPPSHALDMIQGKNNMLLMDSHLEGKFSTEEATVVVNLASKCLQYEPRERPNTKDLVTTLAPLHTKPDVRSHIMLGIPKQEEAPSTPQRPLSAMGEACSRMDLTAIHQILVATHYRDDEGTNEFIDVGTMVSPTVFARRSLCYLLCDQPDPALRDAMQAQCVYPDWPTSFYMQSVALAKLNMHKDAADMLNEAAALEEKRQRGARGS* |
