AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0781070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTTCGATTGATGATTT+TGG 0.207483 5_2:-63364595 Msa0781070:intron
GCAGATTGGTATTTGTTTAT+TGG 0.221536 5_2:-63363048 Msa0781070:intron
GCAGGCTCAGGTTGCGAATA+TGG 0.262383 5_2:-63363162 Msa0781070:CDS
AGTTAGAGTTGCTCAGTTGC+AGG 0.337848 5_2:-63363180 Msa0781070:CDS
TGAAACCGATTTGCGATTGC+AGG 0.350905 5_2:+63364615 None:intergenic
TCTCTCTCAATTGAGTTGTT+TGG 0.356825 5_2:+63364640 None:intergenic
TCAAAGTTGTTCAAGGCATA+TGG 0.370318 5_2:+63362982 None:intergenic
GCTTCTAGGAATCATCATTA+TGG 0.382482 5_2:-63363140 Msa0781070:intron
AGGTTGCGAATATGGCTTCT+AGG 0.400658 5_2:-63363154 Msa0781070:CDS
AGTTGCTCAGTTGCAGGCTC+AGG 0.426888 5_2:-63363174 Msa0781070:CDS
TGGTGGAAAGCGAGTGGGTT+TGG 0.430514 5_2:+63364887 None:intergenic
TGAAAACTTACGAAAAGGTT+AGG 0.442783 5_2:+63364451 None:intergenic
TTCTGTTCAGTTGCATAAGT+TGG 0.451286 5_2:-63363010 Msa0781070:CDS
ATTCGTTCTTCACCGCGACT+TGG 0.452783 5_2:-63364825 Msa0781070:five_prime_UTR
CAAAGTTGTTCAAGGCATAT+GGG 0.454397 5_2:+63362983 None:intergenic
CAAGGTAGAAGAAGAATGAT+TGG 0.467797 5_2:+63364979 None:intergenic
CCGAAAATTGATCGTAATCA+AGG 0.475363 5_2:+63364961 None:intergenic
GACGCGGTGGTCAACGGCGG+TGG 0.478399 5_2:+63364851 None:intergenic
AAGGAGGCTATTGATAGTCT+TGG 0.486802 5_2:-63364507 Msa0781070:CDS
AAAGCGAGTGGGTTTGGTAG+AGG 0.491962 5_2:+63364893 None:intergenic
CAACGGCGGTGGTGCAACTC+CGG 0.501991 5_2:+63364862 None:intergenic
GAAGCTTCTTCTACTTTGCA+AGG 0.516343 5_2:-63364793 Msa0781070:intron
AGAACCTTCTCTACATGCAT+AGG 0.523194 5_2:+63363223 None:intergenic
AGAGACCTGCAATCGCAAAT+CGG 0.551742 5_2:-63364620 Msa0781070:five_prime_UTR
ACTCTAACTTTAGTGATCAA+AGG 0.554954 5_2:+63363194 None:intergenic
GAAGCCTATGCATGTAGAGA+AGG 0.576897 5_2:-63363227 Msa0781070:CDS
AATTTCGACGCGGTGGTCAA+CGG 0.577840 5_2:+63364845 None:intergenic
CCGGCGTGGTGGAAAGCGAG+TGG 0.582991 5_2:+63364881 None:intergenic
CGGCGTGGTGGAAAGCGAGT+GGG 0.600090 5_2:+63364882 None:intergenic
TTCTGACAGCATCCAAGTCG+CGG 0.615165 5_2:+63364813 None:intergenic
GTGGTGCAACTCCGGCGTGG+TGG 0.617063 5_2:+63364870 None:intergenic
CATGTAGAGAAGGTTCTGAA+AGG 0.642317 5_2:-63363217 Msa0781070:CDS
TTCGACGCGGTGGTCAACGG+CGG 0.658384 5_2:+63364848 None:intergenic
AAAGTTGTTCAAGGCATATG+GGG 0.658768 5_2:+63362984 None:intergenic
GCGGTGGTGCAACTCCGGCG+TGG 0.696509 5_2:+63364867 None:intergenic
AAGAACGAATTTCGACGCGG+TGG 0.709622 5_2:+63364838 None:intergenic
CCACTCGCTTTCCACCACGC+CGG 0.719102 5_2:-63364881 Msa0781070:five_prime_UTR
GTGAAGAACGAATTTCGACG+CGG 0.806638 5_2:+63364835 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAAAAATTGTAATTTAA+AGG - chr5_2:63363685-63363704 Msa0781070:intron 10.0%
!! ATAAAAATTGTAATTTAAAG+GGG - chr5_2:63363687-63363706 Msa0781070:intron 10.0%
!! AAAAATAAATAAAAAATGGA+AGG - chr5_2:63364249-63364268 Msa0781070:intron 10.0%
!! CAATAAAAATTGTAATTTAA+AGG - chr5_2:63363685-63363704 Msa0781070:intron 10.0%
!! ATAAAAATTGTAATTTAAAG+GGG - chr5_2:63363687-63363706 Msa0781070:intron 10.0%
!! AAAAATAAATAAAAAATGGA+AGG - chr5_2:63364249-63364268 Msa0781070:intron 10.0%
!! TGTCAAATAGAATATAAAAA+GGG + chr5_2:63363409-63363428 None:intergenic 15.0%
!!! TATATTTGATTTGTATATGT+TGG - chr5_2:63363928-63363947 Msa0781070:intron 15.0%
!! AAATATTGACAGATTAAAAT+TGG + chr5_2:63364206-63364225 None:intergenic 15.0%
!! TGTCAAATAGAATATAAAAA+GGG + chr5_2:63363409-63363428 None:intergenic 15.0%
!!! TATATTTGATTTGTATATGT+TGG - chr5_2:63363928-63363947 Msa0781070:intron 15.0%
!! AAATATTGACAGATTAAAAT+TGG + chr5_2:63364206-63364225 None:intergenic 15.0%
!! ATGATTCAATATTAGAAATG+AGG + chr5_2:63363215-63363234 None:intergenic 20.0%
!!! TATTCATTTTGATCTTTGTT+TGG - chr5_2:63363242-63363261 Msa0781070:intron 20.0%
!! GTCAAATAGAATATAAAAAG+GGG + chr5_2:63363408-63363427 None:intergenic 20.0%
!! GTGTCAAATAGAATATAAAA+AGG + chr5_2:63363410-63363429 None:intergenic 20.0%
!! TGAAATTAAAAGAAAAGAAC+TGG + chr5_2:63363621-63363640 None:intergenic 20.0%
!!! TATTTTATTCTTTTAGTTCC+TGG - chr5_2:63363738-63363757 Msa0781070:intron 20.0%
!! ATTATGTTACAAATCAACAA+AGG + chr5_2:63363973-63363992 None:intergenic 20.0%
!!! CTCATCTAAAACAAATTTTA+CGG - chr5_2:63364018-63364037 Msa0781070:intron 20.0%
!! AGTATTTGAATAAATAAGCA+AGG + chr5_2:63364690-63364709 None:intergenic 20.0%
!!! AACAACTTTGAGATTTTTAT+TGG - chr5_2:63364985-63365004 Msa0781070:five_prime_UTR 20.0%
!! ATGATTCAATATTAGAAATG+AGG + chr5_2:63363215-63363234 None:intergenic 20.0%
!!! TATTCATTTTGATCTTTGTT+TGG - chr5_2:63363242-63363261 Msa0781070:intron 20.0%
!! GTCAAATAGAATATAAAAAG+GGG + chr5_2:63363408-63363427 None:intergenic 20.0%
!! GTGTCAAATAGAATATAAAA+AGG + chr5_2:63363410-63363429 None:intergenic 20.0%
!! TGAAATTAAAAGAAAAGAAC+TGG + chr5_2:63363621-63363640 None:intergenic 20.0%
!!! TATTTTATTCTTTTAGTTCC+TGG - chr5_2:63363738-63363757 Msa0781070:intron 20.0%
!! ATTATGTTACAAATCAACAA+AGG + chr5_2:63363973-63363992 None:intergenic 20.0%
!!! CTCATCTAAAACAAATTTTA+CGG - chr5_2:63364018-63364037 Msa0781070:intron 20.0%
!! AGTATTTGAATAAATAAGCA+AGG + chr5_2:63364690-63364709 None:intergenic 20.0%
!!! AACAACTTTGAGATTTTTAT+TGG - chr5_2:63364985-63365004 Msa0781070:five_prime_UTR 20.0%
!!! TCAATTTCGATTGATGATTT+TGG - chr5_2:63363360-63363379 Msa0781070:intron 25.0%
!! GAATATAAAAAGGGGATTTT+AGG + chr5_2:63363400-63363419 None:intergenic 25.0%
! TGCAACAAAACAAATATTGA+AGG + chr5_2:63363549-63363568 None:intergenic 25.0%
!!! GTTTTCCAAATTGTTGTTAA+AGG - chr5_2:63363886-63363905 Msa0781070:intron 25.0%
! AAACTCCTTTAACAACAATT+TGG + chr5_2:63363894-63363913 None:intergenic 25.0%
!! TTGTTAAAGGAGTTTTACTT+GGG - chr5_2:63363899-63363918 Msa0781070:intron 25.0%
! TTTATGAATTCGTCTATCAA+TGG + chr5_2:63364077-63364096 None:intergenic 25.0%
!!! TCACTCATGAATTTTTTGTT+TGG - chr5_2:63364124-63364143 Msa0781070:intron 25.0%
!! TAGAATTCAAGTTTCAGTAT+TGG + chr5_2:63364477-63364496 None:intergenic 25.0%
! AAAAATCTCAAAGTTGTTCA+AGG + chr5_2:63364983-63365002 None:intergenic 25.0%
!!! TCAATTTCGATTGATGATTT+TGG - chr5_2:63363360-63363379 Msa0781070:intron 25.0%
!! GAATATAAAAAGGGGATTTT+AGG + chr5_2:63363400-63363419 None:intergenic 25.0%
! TGCAACAAAACAAATATTGA+AGG + chr5_2:63363549-63363568 None:intergenic 25.0%
!!! GTTTTCCAAATTGTTGTTAA+AGG - chr5_2:63363886-63363905 Msa0781070:intron 25.0%
! AAACTCCTTTAACAACAATT+TGG + chr5_2:63363894-63363913 None:intergenic 25.0%
!! TTGTTAAAGGAGTTTTACTT+GGG - chr5_2:63363899-63363918 Msa0781070:intron 25.0%
! TTTATGAATTCGTCTATCAA+TGG + chr5_2:63364077-63364096 None:intergenic 25.0%
!!! TCACTCATGAATTTTTTGTT+TGG - chr5_2:63364124-63364143 Msa0781070:intron 25.0%
!! TAGAATTCAAGTTTCAGTAT+TGG + chr5_2:63364477-63364496 None:intergenic 25.0%
! AAAAATCTCAAAGTTGTTCA+AGG + chr5_2:63364983-63365002 None:intergenic 25.0%
! CCTTGATTACGATCAATTTT+CGG - chr5_2:63362994-63363013 Msa0781070:CDS 30.0%
! TTTTACAGACAGAATCACAA+GGG - chr5_2:63363277-63363296 Msa0781070:intron 30.0%
TGAAAACTTACGAAAAGGTT+AGG + chr5_2:63363507-63363526 None:intergenic 30.0%
AGAAGTGAAAACTTACGAAA+AGG + chr5_2:63363512-63363531 None:intergenic 30.0%
AGTTCCTGGTGATTATATTT+AGG - chr5_2:63363752-63363771 Msa0781070:intron 30.0%
CAAACCTAAATATAATCACC+AGG + chr5_2:63363759-63363778 None:intergenic 30.0%
! GTTGTTAAAGGAGTTTTACT+TGG - chr5_2:63363898-63363917 Msa0781070:intron 30.0%
! TGTTAAAGGAGTTTTACTTG+GGG - chr5_2:63363900-63363919 Msa0781070:intron 30.0%
! GTATATGTTGGAGACTTTTA+CGG - chr5_2:63363940-63363959 Msa0781070:intron 30.0%
! ACAGATTAAAATTGGTGACA+GGG + chr5_2:63364198-63364217 None:intergenic 30.0%
! CTGTCAATATTTCTTTTCTG+AGG - chr5_2:63364214-63364233 Msa0781070:intron 30.0%
!!! TTTTGTGTCTTAGCTTTGAT+TGG + chr5_2:63364358-63364377 None:intergenic 30.0%
! AATTAGGAAAGGTAATTTGC+TGG - chr5_2:63364446-63364465 Msa0781070:intron 30.0%
ATACCTGACATATTGCTTAT+TGG - chr5_2:63364569-63364588 Msa0781070:intron 30.0%
TTACCAATAAGCAATATGTC+AGG + chr5_2:63364575-63364594 None:intergenic 30.0%
! ACATATTGCTTATTGGTAAC+AGG - chr5_2:63364576-63364595 Msa0781070:intron 30.0%
ATTTCAGTAAAATATGGAGC+TGG - chr5_2:63364615-63364634 Msa0781070:five_prime_UTR 30.0%
TTTCAGTAAAATATGGAGCT+GGG - chr5_2:63364616-63364635 Msa0781070:five_prime_UTR 30.0%
ACTCTAACTTTAGTGATCAA+AGG + chr5_2:63364764-63364783 None:intergenic 30.0%
!!! CATTCATTGTTGGTTTTGTT+TGG - chr5_2:63364883-63364902 Msa0781070:five_prime_UTR 30.0%
GCAGATTGGTATTTGTTTAT+TGG - chr5_2:63364907-63364926 Msa0781070:five_prime_UTR 30.0%
! TTGAGATTTTTATTGGCATG+AGG - chr5_2:63364992-63365011 Msa0781070:five_prime_UTR 30.0%
! CCTTGATTACGATCAATTTT+CGG - chr5_2:63362994-63363013 Msa0781070:CDS 30.0%
! TTTTACAGACAGAATCACAA+GGG - chr5_2:63363277-63363296 Msa0781070:intron 30.0%
TGAAAACTTACGAAAAGGTT+AGG + chr5_2:63363507-63363526 None:intergenic 30.0%
AGAAGTGAAAACTTACGAAA+AGG + chr5_2:63363512-63363531 None:intergenic 30.0%
AGTTCCTGGTGATTATATTT+AGG - chr5_2:63363752-63363771 Msa0781070:intron 30.0%
CAAACCTAAATATAATCACC+AGG + chr5_2:63363759-63363778 None:intergenic 30.0%
! GTTGTTAAAGGAGTTTTACT+TGG - chr5_2:63363898-63363917 Msa0781070:intron 30.0%
! TGTTAAAGGAGTTTTACTTG+GGG - chr5_2:63363900-63363919 Msa0781070:intron 30.0%
! GTATATGTTGGAGACTTTTA+CGG - chr5_2:63363940-63363959 Msa0781070:intron 30.0%
! ACAGATTAAAATTGGTGACA+GGG + chr5_2:63364198-63364217 None:intergenic 30.0%
! CTGTCAATATTTCTTTTCTG+AGG - chr5_2:63364214-63364233 Msa0781070:intron 30.0%
!!! TTTTGTGTCTTAGCTTTGAT+TGG + chr5_2:63364358-63364377 None:intergenic 30.0%
! AATTAGGAAAGGTAATTTGC+TGG - chr5_2:63364446-63364465 Msa0781070:intron 30.0%
ATACCTGACATATTGCTTAT+TGG - chr5_2:63364569-63364588 Msa0781070:intron 30.0%
TTACCAATAAGCAATATGTC+AGG + chr5_2:63364575-63364594 None:intergenic 30.0%
! ACATATTGCTTATTGGTAAC+AGG - chr5_2:63364576-63364595 Msa0781070:intron 30.0%
ATTTCAGTAAAATATGGAGC+TGG - chr5_2:63364615-63364634 Msa0781070:five_prime_UTR 30.0%
TTTCAGTAAAATATGGAGCT+GGG - chr5_2:63364616-63364635 Msa0781070:five_prime_UTR 30.0%
ACTCTAACTTTAGTGATCAA+AGG + chr5_2:63364764-63364783 None:intergenic 30.0%
!!! CATTCATTGTTGGTTTTGTT+TGG - chr5_2:63364883-63364902 Msa0781070:five_prime_UTR 30.0%
GCAGATTGGTATTTGTTTAT+TGG - chr5_2:63364907-63364926 Msa0781070:five_prime_UTR 30.0%
! TTGAGATTTTTATTGGCATG+AGG - chr5_2:63364992-63365011 Msa0781070:five_prime_UTR 30.0%
GAAGAAGAAGAAGAAGAAGA+GGG + chr5_2:63362942-63362961 None:intergenic 35.0%
AGAAGAAGAAGAAGAAGAAG+AGG + chr5_2:63362943-63362962 None:intergenic 35.0%
CAAGGTAGAAGAAGAATGAT+TGG + chr5_2:63362979-63362998 None:intergenic 35.0%
CCGAAAATTGATCGTAATCA+AGG + chr5_2:63362997-63363016 None:intergenic 35.0%
! GATGATGCTTTTACTTTGGT+GGG + chr5_2:63363026-63363045 None:intergenic 35.0%
! AGACGATGATGCTTTTACTT+TGG + chr5_2:63363030-63363049 None:intergenic 35.0%
! CTTTTACAGACAGAATCACA+AGG - chr5_2:63363276-63363295 Msa0781070:intron 35.0%
! TCTCTCTCAATTGAGTTGTT+TGG + chr5_2:63363318-63363337 None:intergenic 35.0%
GGAATAGAAAAAGAAGAAGC+AGG + chr5_2:63363486-63363505 None:intergenic 35.0%
AATAACATGTAGGTATGGTG+TGG + chr5_2:63363590-63363609 None:intergenic 35.0%
CAGGAAATAACATGTAGGTA+TGG + chr5_2:63363595-63363614 None:intergenic 35.0%
AAAAGAAAAGAACTGGAAGC+AGG + chr5_2:63363614-63363633 None:intergenic 35.0%
!! TAGGTTTGTTGTTCCTTGAA+AGG - chr5_2:63363771-63363790 Msa0781070:intron 35.0%
!! AGGTTTGTTGTTCCTTGAAA+GGG - chr5_2:63363772-63363791 Msa0781070:intron 35.0%
GCAATACAAAATCCCTTTCA+AGG + chr5_2:63363787-63363806 None:intergenic 35.0%
TGCTATTATTTCGTATGCTG+AGG - chr5_2:63363823-63363842 Msa0781070:intron 35.0%
GCTATTATTTCGTATGCTGA+GGG - chr5_2:63363824-63363843 Msa0781070:intron 35.0%
! GTTAAAGGAGTTTTACTTGG+GGG - chr5_2:63363901-63363920 Msa0781070:intron 35.0%
! GACAGATTAAAATTGGTGAC+AGG + chr5_2:63364199-63364218 None:intergenic 35.0%
AAATGGAAGGAACTGTAGTT+TGG - chr5_2:63364262-63364281 Msa0781070:intron 35.0%
GGCGTGATTTCAGTAAAATA+TGG - chr5_2:63364609-63364628 Msa0781070:five_prime_UTR 35.0%
! GCTTCTAGGAATCATCATTA+TGG - chr5_2:63364815-63364834 Msa0781070:five_prime_UTR 35.0%
AATCATCATTATGGTAGCTG+AGG - chr5_2:63364824-63364843 Msa0781070:five_prime_UTR 35.0%
!! GTTCTGGAATCATTCATTGT+TGG - chr5_2:63364873-63364892 Msa0781070:five_prime_UTR 35.0%
TTCTGTTCAGTTGCATAAGT+TGG - chr5_2:63364945-63364964 Msa0781070:five_prime_UTR 35.0%
AAAGTTGTTCAAGGCATATG+GGG + chr5_2:63364974-63364993 None:intergenic 35.0%
CAAAGTTGTTCAAGGCATAT+GGG + chr5_2:63364975-63364994 None:intergenic 35.0%
TCAAAGTTGTTCAAGGCATA+TGG + chr5_2:63364976-63364995 None:intergenic 35.0%
GAAGAAGAAGAAGAAGAAGA+GGG + chr5_2:63362942-63362961 None:intergenic 35.0%
AGAAGAAGAAGAAGAAGAAG+AGG + chr5_2:63362943-63362962 None:intergenic 35.0%
CAAGGTAGAAGAAGAATGAT+TGG + chr5_2:63362979-63362998 None:intergenic 35.0%
CCGAAAATTGATCGTAATCA+AGG + chr5_2:63362997-63363016 None:intergenic 35.0%
! GATGATGCTTTTACTTTGGT+GGG + chr5_2:63363026-63363045 None:intergenic 35.0%
! AGACGATGATGCTTTTACTT+TGG + chr5_2:63363030-63363049 None:intergenic 35.0%
! CTTTTACAGACAGAATCACA+AGG - chr5_2:63363276-63363295 Msa0781070:intron 35.0%
! TCTCTCTCAATTGAGTTGTT+TGG + chr5_2:63363318-63363337 None:intergenic 35.0%
GGAATAGAAAAAGAAGAAGC+AGG + chr5_2:63363486-63363505 None:intergenic 35.0%
AATAACATGTAGGTATGGTG+TGG + chr5_2:63363590-63363609 None:intergenic 35.0%
CAGGAAATAACATGTAGGTA+TGG + chr5_2:63363595-63363614 None:intergenic 35.0%
AAAAGAAAAGAACTGGAAGC+AGG + chr5_2:63363614-63363633 None:intergenic 35.0%
!! TAGGTTTGTTGTTCCTTGAA+AGG - chr5_2:63363771-63363790 Msa0781070:intron 35.0%
!! AGGTTTGTTGTTCCTTGAAA+GGG - chr5_2:63363772-63363791 Msa0781070:intron 35.0%
GCAATACAAAATCCCTTTCA+AGG + chr5_2:63363787-63363806 None:intergenic 35.0%
TGCTATTATTTCGTATGCTG+AGG - chr5_2:63363823-63363842 Msa0781070:intron 35.0%
GCTATTATTTCGTATGCTGA+GGG - chr5_2:63363824-63363843 Msa0781070:intron 35.0%
! GTTAAAGGAGTTTTACTTGG+GGG - chr5_2:63363901-63363920 Msa0781070:intron 35.0%
! GACAGATTAAAATTGGTGAC+AGG + chr5_2:63364199-63364218 None:intergenic 35.0%
AAATGGAAGGAACTGTAGTT+TGG - chr5_2:63364262-63364281 Msa0781070:intron 35.0%
GGCGTGATTTCAGTAAAATA+TGG - chr5_2:63364609-63364628 Msa0781070:five_prime_UTR 35.0%
! GCTTCTAGGAATCATCATTA+TGG - chr5_2:63364815-63364834 Msa0781070:five_prime_UTR 35.0%
AATCATCATTATGGTAGCTG+AGG - chr5_2:63364824-63364843 Msa0781070:five_prime_UTR 35.0%
!! GTTCTGGAATCATTCATTGT+TGG - chr5_2:63364873-63364892 Msa0781070:five_prime_UTR 35.0%
TTCTGTTCAGTTGCATAAGT+TGG - chr5_2:63364945-63364964 Msa0781070:five_prime_UTR 35.0%
AAAGTTGTTCAAGGCATATG+GGG + chr5_2:63364974-63364993 None:intergenic 35.0%
CAAAGTTGTTCAAGGCATAT+GGG + chr5_2:63364975-63364994 None:intergenic 35.0%
TCAAAGTTGTTCAAGGCATA+TGG + chr5_2:63364976-63364995 None:intergenic 35.0%
! GCTTTTACTTTGGTGGGAAA+AGG + chr5_2:63363020-63363039 None:intergenic 40.0%
! CGATGATGCTTTTACTTTGG+TGG + chr5_2:63363027-63363046 None:intergenic 40.0%
GAAGCTTCTTCTACTTTGCA+AGG - chr5_2:63363162-63363181 Msa0781070:CDS 40.0%
! CTTGCTTCTGTTTTGTGACA+AGG - chr5_2:63363429-63363448 Msa0781070:intron 40.0%
! AAGGAGGCTATTGATAGTCT+TGG - chr5_2:63363448-63363467 Msa0781070:intron 40.0%
GGAAGCAGGAAATAACATGT+AGG + chr5_2:63363600-63363619 None:intergenic 40.0%
AGTTTGGATGGCACATATAG+AGG - chr5_2:63364278-63364297 Msa0781070:intron 40.0%
GCACATATAGAGGCTTATGA+TGG - chr5_2:63364288-63364307 Msa0781070:intron 40.0%
! GAAGCTTTGTGGTGTACTTA+CGG - chr5_2:63364316-63364335 Msa0781070:intron 40.0%
TCTTGAGCAGCATTGCAATT+AGG - chr5_2:63364430-63364449 Msa0781070:intron 40.0%
AGCAGCATTGCAATTAGGAA+AGG - chr5_2:63364435-63364454 Msa0781070:intron 40.0%
! TGCTTATTGGTAACAGGTGT+AGG - chr5_2:63364582-63364601 Msa0781070:intron 40.0%
!! TTGGTAACAGGTGTAGGTTT+TGG - chr5_2:63364588-63364607 Msa0781070:intron 40.0%
AAAATATGGAGCTGGGAATG+TGG - chr5_2:63364623-63364642 Msa0781070:five_prime_UTR 40.0%
GCTTGAAGATTCGAACGAAA+GGG - chr5_2:63364645-63364664 Msa0781070:five_prime_UTR 40.0%
CTTGAAGATTCGAACGAAAG+GGG - chr5_2:63364646-63364665 Msa0781070:five_prime_UTR 40.0%
AGAACCTTCTCTACATGCAT+AGG + chr5_2:63364735-63364754 None:intergenic 40.0%
!! CATGTAGAGAAGGTTCTGAA+AGG - chr5_2:63364738-63364757 Msa0781070:intron 40.0%
ATGGATAGGTTTATGTCTGC+AGG - chr5_2:63364851-63364870 Msa0781070:five_prime_UTR 40.0%
!!! TGGTTTTGTTTGGTGCAGAT+TGG - chr5_2:63364893-63364912 Msa0781070:five_prime_UTR 40.0%
! GCTTTTACTTTGGTGGGAAA+AGG + chr5_2:63363020-63363039 None:intergenic 40.0%
! CGATGATGCTTTTACTTTGG+TGG + chr5_2:63363027-63363046 None:intergenic 40.0%
GAAGCTTCTTCTACTTTGCA+AGG - chr5_2:63363162-63363181 Msa0781070:CDS 40.0%
! CTTGCTTCTGTTTTGTGACA+AGG - chr5_2:63363429-63363448 Msa0781070:intron 40.0%
! AAGGAGGCTATTGATAGTCT+TGG - chr5_2:63363448-63363467 Msa0781070:intron 40.0%
GGAAGCAGGAAATAACATGT+AGG + chr5_2:63363600-63363619 None:intergenic 40.0%
AGTTTGGATGGCACATATAG+AGG - chr5_2:63364278-63364297 Msa0781070:intron 40.0%
GCACATATAGAGGCTTATGA+TGG - chr5_2:63364288-63364307 Msa0781070:intron 40.0%
! GAAGCTTTGTGGTGTACTTA+CGG - chr5_2:63364316-63364335 Msa0781070:intron 40.0%
TCTTGAGCAGCATTGCAATT+AGG - chr5_2:63364430-63364449 Msa0781070:intron 40.0%
AGCAGCATTGCAATTAGGAA+AGG - chr5_2:63364435-63364454 Msa0781070:intron 40.0%
! TGCTTATTGGTAACAGGTGT+AGG - chr5_2:63364582-63364601 Msa0781070:intron 40.0%
!! TTGGTAACAGGTGTAGGTTT+TGG - chr5_2:63364588-63364607 Msa0781070:intron 40.0%
AAAATATGGAGCTGGGAATG+TGG - chr5_2:63364623-63364642 Msa0781070:five_prime_UTR 40.0%
GCTTGAAGATTCGAACGAAA+GGG - chr5_2:63364645-63364664 Msa0781070:five_prime_UTR 40.0%
CTTGAAGATTCGAACGAAAG+GGG - chr5_2:63364646-63364665 Msa0781070:five_prime_UTR 40.0%
AGAACCTTCTCTACATGCAT+AGG + chr5_2:63364735-63364754 None:intergenic 40.0%
!! CATGTAGAGAAGGTTCTGAA+AGG - chr5_2:63364738-63364757 Msa0781070:intron 40.0%
ATGGATAGGTTTATGTCTGC+AGG - chr5_2:63364851-63364870 Msa0781070:five_prime_UTR 40.0%
!!! TGGTTTTGTTTGGTGCAGAT+TGG - chr5_2:63364893-63364912 Msa0781070:five_prime_UTR 40.0%
GTGAAGAACGAATTTCGACG+CGG + chr5_2:63363123-63363142 None:intergenic 45.0%
AGAGACCTGCAATCGCAAAT+CGG - chr5_2:63363335-63363354 Msa0781070:intron 45.0%
!! TGAAACCGATTTGCGATTGC+AGG + chr5_2:63363343-63363362 None:intergenic 45.0%
! GCTTCTGTTTTGTGACAAGG+AGG - chr5_2:63363432-63363451 Msa0781070:intron 45.0%
GGAAGGAACTGTAGTTTGGA+TGG - chr5_2:63364266-63364285 Msa0781070:intron 45.0%
! TGATGGTGTCTGAAGCTTTG+TGG - chr5_2:63364305-63364324 Msa0781070:intron 45.0%
GGCTTGAAGATTCGAACGAA+AGG - chr5_2:63364644-63364663 Msa0781070:five_prime_UTR 45.0%
AAGATTCGAACGAAAGGGGA+CGG - chr5_2:63364650-63364669 Msa0781070:five_prime_UTR 45.0%
GAAGCCTATGCATGTAGAGA+AGG - chr5_2:63364728-63364747 Msa0781070:intron 45.0%
AGTTAGAGTTGCTCAGTTGC+AGG - chr5_2:63364775-63364794 Msa0781070:intron 45.0%
AGGTTGCGAATATGGCTTCT+AGG - chr5_2:63364801-63364820 Msa0781070:five_prime_UTR 45.0%
CATCATTATGGTAGCTGAGG+AGG - chr5_2:63364827-63364846 Msa0781070:five_prime_UTR 45.0%
AGGTTTATGTCTGCAGGTTC+TGG - chr5_2:63364857-63364876 Msa0781070:five_prime_UTR 45.0%
GTGAAGAACGAATTTCGACG+CGG + chr5_2:63363123-63363142 None:intergenic 45.0%
AGAGACCTGCAATCGCAAAT+CGG - chr5_2:63363335-63363354 Msa0781070:intron 45.0%
!! TGAAACCGATTTGCGATTGC+AGG + chr5_2:63363343-63363362 None:intergenic 45.0%
! GCTTCTGTTTTGTGACAAGG+AGG - chr5_2:63363432-63363451 Msa0781070:intron 45.0%
GGAAGGAACTGTAGTTTGGA+TGG - chr5_2:63364266-63364285 Msa0781070:intron 45.0%
! TGATGGTGTCTGAAGCTTTG+TGG - chr5_2:63364305-63364324 Msa0781070:intron 45.0%
GGCTTGAAGATTCGAACGAA+AGG - chr5_2:63364644-63364663 Msa0781070:five_prime_UTR 45.0%
AAGATTCGAACGAAAGGGGA+CGG - chr5_2:63364650-63364669 Msa0781070:five_prime_UTR 45.0%
GAAGCCTATGCATGTAGAGA+AGG - chr5_2:63364728-63364747 Msa0781070:intron 45.0%
AGTTAGAGTTGCTCAGTTGC+AGG - chr5_2:63364775-63364794 Msa0781070:intron 45.0%
AGGTTGCGAATATGGCTTCT+AGG - chr5_2:63364801-63364820 Msa0781070:five_prime_UTR 45.0%
CATCATTATGGTAGCTGAGG+AGG - chr5_2:63364827-63364846 Msa0781070:five_prime_UTR 45.0%
AGGTTTATGTCTGCAGGTTC+TGG - chr5_2:63364857-63364876 Msa0781070:five_prime_UTR 45.0%
!!! TAATTAAATTAATTTTTTCT+AGG + chr5_2:63363652-63363671 None:intergenic 5.0%
!! AATAAAAATTGTAATTTAAA+GGG - chr5_2:63363686-63363705 Msa0781070:intron 5.0%
!! TGTAAAAAATAAATAAAAAA+TGG - chr5_2:63364245-63364264 Msa0781070:intron 5.0%
!!! TAATTAAATTAATTTTTTCT+AGG + chr5_2:63363652-63363671 None:intergenic 5.0%
!! AATAAAAATTGTAATTTAAA+GGG - chr5_2:63363686-63363705 Msa0781070:intron 5.0%
!! TGTAAAAAATAAATAAAAAA+TGG - chr5_2:63364245-63364264 Msa0781070:intron 5.0%
! AAAGCGAGTGGGTTTGGTAG+AGG + chr5_2:63363065-63363084 None:intergenic 50.0%
AATTTCGACGCGGTGGTCAA+CGG + chr5_2:63363113-63363132 None:intergenic 50.0%
AAGAACGAATTTCGACGCGG+TGG + chr5_2:63363120-63363139 None:intergenic 50.0%
ATTCGTTCTTCACCGCGACT+TGG - chr5_2:63363130-63363149 Msa0781070:intron 50.0%
TTCTGACAGCATCCAAGTCG+CGG + chr5_2:63363145-63363164 None:intergenic 50.0%
TGCTCTCCAAGCAGTTGTGA+TGG - chr5_2:63364525-63364544 Msa0781070:intron 50.0%
TTATGGTAGCTGAGGAGGCA+TGG - chr5_2:63364832-63364851 Msa0781070:five_prime_UTR 50.0%
! AAAGCGAGTGGGTTTGGTAG+AGG + chr5_2:63363065-63363084 None:intergenic 50.0%
AATTTCGACGCGGTGGTCAA+CGG + chr5_2:63363113-63363132 None:intergenic 50.0%
AAGAACGAATTTCGACGCGG+TGG + chr5_2:63363120-63363139 None:intergenic 50.0%
ATTCGTTCTTCACCGCGACT+TGG - chr5_2:63363130-63363149 Msa0781070:intron 50.0%
TTCTGACAGCATCCAAGTCG+CGG + chr5_2:63363145-63363164 None:intergenic 50.0%
TGCTCTCCAAGCAGTTGTGA+TGG - chr5_2:63364525-63364544 Msa0781070:intron 50.0%
TTATGGTAGCTGAGGAGGCA+TGG - chr5_2:63364832-63364851 Msa0781070:five_prime_UTR 50.0%
! TGGTGGAAAGCGAGTGGGTT+TGG + chr5_2:63363071-63363090 None:intergenic 55.0%
TCGTGGCCATCACAACTGCT+TGG + chr5_2:63364534-63364553 None:intergenic 55.0%
TGTGATGGCCACGAATCTGC+TGG - chr5_2:63364540-63364559 Msa0781070:intron 55.0%
ATCGCACACCAGCAGATTCG+TGG + chr5_2:63364551-63364570 None:intergenic 55.0%
AGTTGCTCAGTTGCAGGCTC+AGG - chr5_2:63364781-63364800 Msa0781070:intron 55.0%
! GCAGGCTCAGGTTGCGAATA+TGG - chr5_2:63364793-63364812 Msa0781070:intron 55.0%
! GTAGCTGAGGAGGCATGGAT+AGG - chr5_2:63364837-63364856 Msa0781070:five_prime_UTR 55.0%
! TGGTGGAAAGCGAGTGGGTT+TGG + chr5_2:63363071-63363090 None:intergenic 55.0%
TCGTGGCCATCACAACTGCT+TGG + chr5_2:63364534-63364553 None:intergenic 55.0%
TGTGATGGCCACGAATCTGC+TGG - chr5_2:63364540-63364559 Msa0781070:intron 55.0%
ATCGCACACCAGCAGATTCG+TGG + chr5_2:63364551-63364570 None:intergenic 55.0%
AGTTGCTCAGTTGCAGGCTC+AGG - chr5_2:63364781-63364800 Msa0781070:intron 55.0%
! GCAGGCTCAGGTTGCGAATA+TGG - chr5_2:63364793-63364812 Msa0781070:intron 55.0%
! GTAGCTGAGGAGGCATGGAT+AGG - chr5_2:63364837-63364856 Msa0781070:five_prime_UTR 55.0%
! CGGCGTGGTGGAAAGCGAGT+GGG + chr5_2:63363076-63363095 None:intergenic 65.0%
CCACTCGCTTTCCACCACGC+CGG - chr5_2:63363074-63363093 Msa0781070:intron 65.0%
CAACGGCGGTGGTGCAACTC+CGG + chr5_2:63363096-63363115 None:intergenic 65.0%
TTCGACGCGGTGGTCAACGG+CGG + chr5_2:63363110-63363129 None:intergenic 65.0%
! CGGCGTGGTGGAAAGCGAGT+GGG + chr5_2:63363076-63363095 None:intergenic 65.0%
CCACTCGCTTTCCACCACGC+CGG - chr5_2:63363074-63363093 Msa0781070:intron 65.0%
CAACGGCGGTGGTGCAACTC+CGG + chr5_2:63363096-63363115 None:intergenic 65.0%
TTCGACGCGGTGGTCAACGG+CGG + chr5_2:63363110-63363129 None:intergenic 65.0%
! CCGGCGTGGTGGAAAGCGAG+TGG + chr5_2:63363077-63363096 None:intergenic 70.0%
GTGGTGCAACTCCGGCGTGG+TGG + chr5_2:63363088-63363107 None:intergenic 70.0%
! CCGGCGTGGTGGAAAGCGAG+TGG + chr5_2:63363077-63363096 None:intergenic 70.0%
GTGGTGCAACTCCGGCGTGG+TGG + chr5_2:63363088-63363107 None:intergenic 70.0%
GCGGTGGTGCAACTCCGGCG+TGG + chr5_2:63363091-63363110 None:intergenic 75.0%
GACGCGGTGGTCAACGGCGG+TGG + chr5_2:63363107-63363126 None:intergenic 75.0%
GCGGTGGTGCAACTCCGGCG+TGG + chr5_2:63363091-63363110 None:intergenic 75.0%
GACGCGGTGGTCAACGGCGG+TGG + chr5_2:63363107-63363126 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 63362927 63365050 63362927 ID=Msa0781070;Name=Msa0781070
chr5_2 mRNA 63362927 63365050 63362927 ID=Msa0781070-mRNA-1;Parent=Msa0781070;Name=Msa0781070-mRNA-1;_AED=0.37;_eAED=0.37;_QI=345|0.25|0.2|1|0.75|0.8|5|0|110
chr5_2 exon 63362927 63363066 63362927 ID=Msa0781070-mRNA-1:exon:18845;Parent=Msa0781070-mRNA-1
chr5_2 exon 63363141 63363259 63363141 ID=Msa0781070-mRNA-1:exon:18844;Parent=Msa0781070-mRNA-1
chr5_2 exon 63364462 63364526 63364462 ID=Msa0781070-mRNA-1:exon:18843;Parent=Msa0781070-mRNA-1
chr5_2 exon 63364596 63364692 63364596 ID=Msa0781070-mRNA-1:exon:18842;Parent=Msa0781070-mRNA-1
chr5_2 exon 63364794 63365050 63364794 ID=Msa0781070-mRNA-1:exon:18841;Parent=Msa0781070-mRNA-1
chr5_2 five_prime_UTR 63364794 63365050 63364794 ID=Msa0781070-mRNA-1:five_prime_utr;Parent=Msa0781070-mRNA-1
chr5_2 five_prime_UTR 63364605 63364692 63364605 ID=Msa0781070-mRNA-1:five_prime_utr;Parent=Msa0781070-mRNA-1
chr5_2 CDS 63364596 63364604 63364596 ID=Msa0781070-mRNA-1:cds;Parent=Msa0781070-mRNA-1
chr5_2 CDS 63364462 63364526 63364462 ID=Msa0781070-mRNA-1:cds;Parent=Msa0781070-mRNA-1
chr5_2 CDS 63363141 63363259 63363141 ID=Msa0781070-mRNA-1:cds;Parent=Msa0781070-mRNA-1
chr5_2 CDS 63362927 63363066 63362927 ID=Msa0781070-mRNA-1:cds;Parent=Msa0781070-mRNA-1
Gene Sequence

>Msa0781070

ATGATTTTGGAGGCTATTGATAGTCTTGGAAATCTTCTGCTCCTGCTTCTTCTTTTTCTATTCCTAACCTTTTCATGTTTCATAGAAGCCTATGCATGTAGAGAAGGTTCTGAAAGGCCTTTGATCACTAAAGTTAGAGTTGCTCAGTTGCAGGCTCAGGTTGCGAATATGGCTTCTAGGAATCATCATTATGATTGGTATTTGTTTATTGGCTTTTTAGTCGCTGCTTCTGTTCAGTTGCATAAGTTGGCAGCCCCATATGCCTTGAACAACTTTGAGATTTTTATTGGCATGAGGCTGTACGATTGTTCTTATACAGATTTAATTATATGA
Protein sequence

>Msa0781070

MILEAIDSLGNLLLLLLLFLFLTFSCFIEAYACREGSERPLITKVRVAQLQAQVANMASRNHHYDWYLFIGFLVAASVQLHKLAAPYALNNFEIFIGMRLYDCSYTDLII*