AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0783390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0783390 MtrunA17_Chr5g0438411 79.699 133 14 2 1 120 1 133 9.14e-35 116
Msa0783390 MtrunA17_Chr5g0438511 55.085 118 41 2 1 118 1 106 8.14e-21 82.4
Msa0783390 MtrunA17_Chr5g0438501 38.462 169 46 3 1 120 1 160 6.24e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCCTTATCCTAGGTTTAT+TGG 0.152765 5_2:+67336089 Msa0783390:CDS
GAATTAAATGATGCAAAATA+TGG 0.269595 5_2:+67337101 Msa0783390:CDS
ACAAAATATTTCATTTGAAT+TGG 0.303512 5_2:+67336040 Msa0783390:five_prime_UTR
CCTGGTCGTGGTGGTAATTA+TGG 0.320779 5_2:+67337143 Msa0783390:CDS
TCTTATCTCCTCAGAGGTTT+CGG 0.325236 5_2:+67336122 Msa0783390:CDS
ACATCCTCTAATACCCAAAA+AGG 0.335650 5_2:+67336162 Msa0783390:CDS
TGTTGCGGTGACAGGTATTA+TGG 0.351573 5_2:+67337236 Msa0783390:CDS
TAATTACCACCACGGTTTCC+AGG 0.361900 5_2:-67337200 None:intergenic
TAACCACCACGACGGTTTCC+AGG 0.401563 5_2:-67337176 None:intergenic
GTACTCCTTTGTATGTGCTT+CGG 0.416317 5_2:+67337479 Msa0783390:three_prime_UTR
CTCCTCAGAGGTTTCGGCTA+GGG 0.435270 5_2:+67336128 Msa0783390:CDS
AATTATGGTGGTGGTTATCC+TGG 0.457834 5_2:+67337158 Msa0783390:CDS
GCAAAATATGGAAGCTATGG+TGG 0.487275 5_2:+67337113 Msa0783390:CDS
GCTATGGCTGTTGCGGTGAC+AGG 0.495730 5_2:+67337228 Msa0783390:CDS
TAGCCAATAAACCTAGGATA+AGG 0.503132 5_2:-67336092 None:intergenic
CGTTGTGTGTTTATCAAGAG+TGG 0.503351 5_2:+67337391 Msa0783390:three_prime_UTR
GTTGCGGTGACAGGTATTAT+GGG 0.504359 5_2:+67337237 Msa0783390:CDS
GATCCACAAAGCAATACAAA+AGG 0.508605 5_2:-67337452 None:intergenic
AACCGTCGTGGTGGTTACCC+TGG 0.514713 5_2:+67337182 Msa0783390:CDS
AATTACCACCACGGTTTCCA+GGG 0.523853 5_2:-67337199 None:intergenic
CATAGTGATATCATCATGTA+TGG 0.526664 5_2:+67337333 Msa0783390:three_prime_UTR
CGTGGTGGTAATTATGGTGG+TGG 0.530246 5_2:+67337149 Msa0783390:CDS
GATGCAAAATATGGAAGCTA+TGG 0.536370 5_2:+67337110 Msa0783390:CDS
CAAAATATGGAAGCTATGGT+GGG 0.547029 5_2:+67337114 Msa0783390:CDS
TTTGAATTGGTATTGAACAA+TGG 0.556223 5_2:+67336053 Msa0783390:exon
CATTCTTCTTATCTCCTCAG+AGG 0.558070 5_2:+67336116 Msa0783390:CDS
AGTGTTGTTCTCAGTTTGCA+CGG 0.568848 5_2:-67337300 None:intergenic
GGTGGTAATTATTGTCGCTA+TGG 0.572979 5_2:+67337212 Msa0783390:CDS
TCTCCTCAGAGGTTTCGGCT+AGG 0.577778 5_2:+67336127 Msa0783390:CDS
TGATATAATAAATAAAAGCA+TGG 0.581622 5_2:-67337618 None:intergenic
GGTATTATGGGAGTTGCAGA+AGG 0.591131 5_2:+67337249 Msa0783390:CDS
CCATAATTACCACCACGACC+AGG 0.597203 5_2:-67337143 None:intergenic
TATTGTCGCTATGGCTGTTG+CGG 0.598460 5_2:+67337221 Msa0783390:CDS
ATATCAAACTATCGTTAAGA+CGG 0.607431 5_2:+67337635 Msa0783390:three_prime_UTR
GAATGATAGCCAATAAACCT+AGG 0.610349 5_2:-67336098 None:intergenic
AAAATATGGAAGCTATGGTG+GGG 0.614234 5_2:+67337115 Msa0783390:CDS
AGAAGGTGTTGCTCATATGC+TGG 0.617122 5_2:+67337266 Msa0783390:CDS
AAATATGGAAGCTATGGTGG+GGG 0.630498 5_2:+67337116 Msa0783390:CDS
GGTCGTGGTGGTAATTATGG+TGG 0.644801 5_2:+67337146 Msa0783390:CDS
TATCCTGGAAACCGTCGTGG+TGG 0.656242 5_2:+67337173 Msa0783390:CDS
AATATCCGAAGCACATACAA+AGG 0.658664 5_2:-67337484 None:intergenic
GTCCCTAGCCGAAACCTCTG+AGG 0.664836 5_2:-67336130 None:intergenic
TTCCAGGGTAACCACCACGA+CGG 0.693918 5_2:-67337184 None:intergenic
AGCGACAATAATTACCACCA+CGG 0.696105 5_2:-67337208 None:intergenic
GGTTATCCTGGAAACCGTCG+TGG 0.701390 5_2:+67337170 Msa0783390:CDS
GGTGGTTACCCTGGAAACCG+TGG 0.707418 5_2:+67337191 Msa0783390:CDS
GGTTACCCTGGAAACCGTGG+TGG 0.745370 5_2:+67337194 Msa0783390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTATGTTTTTTATTT+TGG - chr5_2:67336366-67336385 None:intergenic 10.0%
!!! TCATTTTTACAAATAAAAAA+AGG - chr5_2:67337023-67337042 None:intergenic 10.0%
!!! TCAATTTATGTTTTTTATTT+TGG - chr5_2:67336366-67336385 None:intergenic 10.0%
!!! TCATTTTTACAAATAAAAAA+AGG - chr5_2:67337023-67337042 None:intergenic 10.0%
!! ACAAAATATTTCATTTGAAT+TGG + chr5_2:67336040-67336059 Msa0783390:five_prime_UTR 15.0%
!!! AGTTAAATGTGTTATTTTTT+GGG - chr5_2:67336938-67336957 None:intergenic 15.0%
!!! TAGTTAAATGTGTTATTTTT+TGG - chr5_2:67336939-67336958 None:intergenic 15.0%
!! TCTAAAGAGTTTAAAAAAAA+GGG - chr5_2:67337510-67337529 None:intergenic 15.0%
!! ATCTAAAGAGTTTAAAAAAA+AGG - chr5_2:67337511-67337530 None:intergenic 15.0%
!! TGATATAATAAATAAAAGCA+TGG - chr5_2:67337621-67337640 None:intergenic 15.0%
!! ACAAAATATTTCATTTGAAT+TGG + chr5_2:67336040-67336059 Msa0783390:five_prime_UTR 15.0%
!!! AGTTAAATGTGTTATTTTTT+GGG - chr5_2:67336938-67336957 None:intergenic 15.0%
!!! TAGTTAAATGTGTTATTTTT+TGG - chr5_2:67336939-67336958 None:intergenic 15.0%
!! TCTAAAGAGTTTAAAAAAAA+GGG - chr5_2:67337510-67337529 None:intergenic 15.0%
!! ATCTAAAGAGTTTAAAAAAA+AGG - chr5_2:67337511-67337530 None:intergenic 15.0%
!! TGATATAATAAATAAAAGCA+TGG - chr5_2:67337621-67337640 None:intergenic 15.0%
!!! TAAAAATGAGTGTTTTTAGA+TGG - chr5_2:67336448-67336467 None:intergenic 20.0%
!!! TCATTTCAACAAATTTTGAT+TGG + chr5_2:67336520-67336539 Msa0783390:intron 20.0%
!!! ACACTACTGTTTTTTTTTTT+TGG + chr5_2:67336593-67336612 Msa0783390:intron 20.0%
!!! TTTTTTTTTTGGAACTTCTA+CGG + chr5_2:67336604-67336623 Msa0783390:intron 20.0%
!!! GTTAAATGTGTTATTTTTTG+GGG - chr5_2:67336937-67336956 None:intergenic 20.0%
!! GAATTAAATGATGCAAAATA+TGG + chr5_2:67337101-67337120 Msa0783390:CDS 20.0%
!!! GATGAACTTTTTTAAAAAAG+CGG + chr5_2:67337584-67337603 Msa0783390:three_prime_UTR 20.0%
!!! TAAAAATGAGTGTTTTTAGA+TGG - chr5_2:67336448-67336467 None:intergenic 20.0%
!!! TCATTTCAACAAATTTTGAT+TGG + chr5_2:67336520-67336539 Msa0783390:intron 20.0%
!!! ACACTACTGTTTTTTTTTTT+TGG + chr5_2:67336593-67336612 Msa0783390:intron 20.0%
!!! TTTTTTTTTTGGAACTTCTA+CGG + chr5_2:67336604-67336623 Msa0783390:intron 20.0%
!!! GTTAAATGTGTTATTTTTTG+GGG - chr5_2:67336937-67336956 None:intergenic 20.0%
!! GAATTAAATGATGCAAAATA+TGG + chr5_2:67337101-67337120 Msa0783390:CDS 20.0%
!!! GATGAACTTTTTTAAAAAAG+CGG + chr5_2:67337584-67337603 Msa0783390:three_prime_UTR 20.0%
!! TTTGAATTGGTATTGAACAA+TGG + chr5_2:67336053-67336072 Msa0783390:exon 25.0%
!! ATGTATAATCTCACCTTTTT+GGG - chr5_2:67336178-67336197 None:intergenic 25.0%
!! AATGTATAATCTCACCTTTT+TGG - chr5_2:67336179-67336198 None:intergenic 25.0%
! TATAAAATGTATCATGTCAC+CGG - chr5_2:67336268-67336287 None:intergenic 25.0%
!!! ATACCTTAGTGTTTTTAAGT+GGG - chr5_2:67336409-67336428 None:intergenic 25.0%
!!! TATACCTTAGTGTTTTTAAG+TGG - chr5_2:67336410-67336429 None:intergenic 25.0%
! AAAGTGGATATAAATTAGTG+AGG - chr5_2:67336498-67336517 None:intergenic 25.0%
!! ATTTGTTGAAATGATGAAAG+TGG - chr5_2:67336514-67336533 None:intergenic 25.0%
!!! TTTTATTTCGTCGACATTTT+TGG + chr5_2:67336862-67336881 Msa0783390:intron 25.0%
!!! ACCAATACACACAAATTTTT+TGG + chr5_2:67336981-67337000 Msa0783390:intron 25.0%
! ACCAAAAAATTTGTGTGTAT+TGG - chr5_2:67336985-67337004 None:intergenic 25.0%
! ATATCAAACTATCGTTAAGA+CGG + chr5_2:67337635-67337654 Msa0783390:three_prime_UTR 25.0%
!! TTTGAATTGGTATTGAACAA+TGG + chr5_2:67336053-67336072 Msa0783390:exon 25.0%
!! ATGTATAATCTCACCTTTTT+GGG - chr5_2:67336178-67336197 None:intergenic 25.0%
!! AATGTATAATCTCACCTTTT+TGG - chr5_2:67336179-67336198 None:intergenic 25.0%
! TATAAAATGTATCATGTCAC+CGG - chr5_2:67336268-67336287 None:intergenic 25.0%
!!! ATACCTTAGTGTTTTTAAGT+GGG - chr5_2:67336409-67336428 None:intergenic 25.0%
!!! TATACCTTAGTGTTTTTAAG+TGG - chr5_2:67336410-67336429 None:intergenic 25.0%
! AAAGTGGATATAAATTAGTG+AGG - chr5_2:67336498-67336517 None:intergenic 25.0%
!! ATTTGTTGAAATGATGAAAG+TGG - chr5_2:67336514-67336533 None:intergenic 25.0%
!!! TTTTATTTCGTCGACATTTT+TGG + chr5_2:67336862-67336881 Msa0783390:intron 25.0%
!!! ACCAATACACACAAATTTTT+TGG + chr5_2:67336981-67337000 Msa0783390:intron 25.0%
! ACCAAAAAATTTGTGTGTAT+TGG - chr5_2:67336985-67337004 None:intergenic 25.0%
! ATATCAAACTATCGTTAAGA+CGG + chr5_2:67337635-67337654 Msa0783390:three_prime_UTR 25.0%
AAAAATGCAATCCTTATCCT+AGG + chr5_2:67336081-67336100 Msa0783390:CDS 30.0%
AATCCTTATCCTAGGTTTAT+TGG + chr5_2:67336089-67336108 Msa0783390:CDS 30.0%
!! TCTGAAGGCATTATTATCAA+GGG - chr5_2:67336241-67336260 None:intergenic 30.0%
!! TTCTGAAGGCATTATTATCA+AGG - chr5_2:67336242-67336261 None:intergenic 30.0%
TGGATATAAATTAGTGAGGT+GGG - chr5_2:67336494-67336513 None:intergenic 30.0%
!!! GAATTTTGAAGTAAAAGTGC+TGG - chr5_2:67336652-67336671 None:intergenic 30.0%
CATAGTGATATCATCATGTA+TGG + chr5_2:67337333-67337352 Msa0783390:three_prime_UTR 30.0%
! TTTCCTTTTGTATTGCTTTG+TGG + chr5_2:67337449-67337468 Msa0783390:three_prime_UTR 30.0%
AAAAAGCAGATAAACGGATA+TGG - chr5_2:67337666-67337685 None:intergenic 30.0%
AAAAATGCAATCCTTATCCT+AGG + chr5_2:67336081-67336100 Msa0783390:CDS 30.0%
AATCCTTATCCTAGGTTTAT+TGG + chr5_2:67336089-67336108 Msa0783390:CDS 30.0%
!! TCTGAAGGCATTATTATCAA+GGG - chr5_2:67336241-67336260 None:intergenic 30.0%
!! TTCTGAAGGCATTATTATCA+AGG - chr5_2:67336242-67336261 None:intergenic 30.0%
TGGATATAAATTAGTGAGGT+GGG - chr5_2:67336494-67336513 None:intergenic 30.0%
!!! GAATTTTGAAGTAAAAGTGC+TGG - chr5_2:67336652-67336671 None:intergenic 30.0%
CATAGTGATATCATCATGTA+TGG + chr5_2:67337333-67337352 Msa0783390:three_prime_UTR 30.0%
! TTTCCTTTTGTATTGCTTTG+TGG + chr5_2:67337449-67337468 Msa0783390:three_prime_UTR 30.0%
AAAAAGCAGATAAACGGATA+TGG - chr5_2:67337666-67337685 None:intergenic 30.0%
TAGCCAATAAACCTAGGATA+AGG - chr5_2:67336095-67336114 None:intergenic 35.0%
GAATGATAGCCAATAAACCT+AGG - chr5_2:67336101-67336120 None:intergenic 35.0%
ACATCCTCTAATACCCAAAA+AGG + chr5_2:67336162-67336181 Msa0783390:CDS 35.0%
AATAATGCCTTCAGAAATGC+CGG + chr5_2:67336246-67336265 Msa0783390:intron 35.0%
CCATGAAATAGAGCTTCTAA+CGG - chr5_2:67336323-67336342 None:intergenic 35.0%
CGTTAGAAGCTCTATTTCAT+GGG + chr5_2:67336321-67336340 Msa0783390:intron 35.0%
GTTAGAAGCTCTATTTCATG+GGG + chr5_2:67336322-67336341 Msa0783390:intron 35.0%
ACACCCACTTAAAAACACTA+AGG + chr5_2:67336403-67336422 Msa0783390:intron 35.0%
GTGGATATAAATTAGTGAGG+TGG - chr5_2:67336495-67336514 None:intergenic 35.0%
GATGCAAAATATGGAAGCTA+TGG + chr5_2:67337110-67337129 Msa0783390:CDS 35.0%
CAAAATATGGAAGCTATGGT+GGG + chr5_2:67337114-67337133 Msa0783390:CDS 35.0%
AAAATATGGAAGCTATGGTG+GGG + chr5_2:67337115-67337134 Msa0783390:CDS 35.0%
GATCCACAAAGCAATACAAA+AGG - chr5_2:67337455-67337474 None:intergenic 35.0%
AATATCCGAAGCACATACAA+AGG - chr5_2:67337487-67337506 None:intergenic 35.0%
TAGCCAATAAACCTAGGATA+AGG - chr5_2:67336095-67336114 None:intergenic 35.0%
GAATGATAGCCAATAAACCT+AGG - chr5_2:67336101-67336120 None:intergenic 35.0%
ACATCCTCTAATACCCAAAA+AGG + chr5_2:67336162-67336181 Msa0783390:CDS 35.0%
AATAATGCCTTCAGAAATGC+CGG + chr5_2:67336246-67336265 Msa0783390:intron 35.0%
CCATGAAATAGAGCTTCTAA+CGG - chr5_2:67336323-67336342 None:intergenic 35.0%
CGTTAGAAGCTCTATTTCAT+GGG + chr5_2:67336321-67336340 Msa0783390:intron 35.0%
GTTAGAAGCTCTATTTCATG+GGG + chr5_2:67336322-67336341 Msa0783390:intron 35.0%
ACACCCACTTAAAAACACTA+AGG + chr5_2:67336403-67336422 Msa0783390:intron 35.0%
GTGGATATAAATTAGTGAGG+TGG - chr5_2:67336495-67336514 None:intergenic 35.0%
GATGCAAAATATGGAAGCTA+TGG + chr5_2:67337110-67337129 Msa0783390:CDS 35.0%
CAAAATATGGAAGCTATGGT+GGG + chr5_2:67337114-67337133 Msa0783390:CDS 35.0%
AAAATATGGAAGCTATGGTG+GGG + chr5_2:67337115-67337134 Msa0783390:CDS 35.0%
GATCCACAAAGCAATACAAA+AGG - chr5_2:67337455-67337474 None:intergenic 35.0%
AATATCCGAAGCACATACAA+AGG - chr5_2:67337487-67337506 None:intergenic 35.0%
CATTCTTCTTATCTCCTCAG+AGG + chr5_2:67336116-67336135 Msa0783390:CDS 40.0%
TCTTATCTCCTCAGAGGTTT+CGG + chr5_2:67336122-67336141 Msa0783390:CDS 40.0%
!!! CTCACCTTTTTGGGTATTAG+AGG - chr5_2:67336169-67336188 None:intergenic 40.0%
TAACGGTGGAGTCATTATGA+AGG - chr5_2:67336306-67336325 None:intergenic 40.0%
TGAAATAGAGCTTCTAACGG+TGG - chr5_2:67336320-67336339 None:intergenic 40.0%
CCGTTAGAAGCTCTATTTCA+TGG + chr5_2:67336320-67336339 Msa0783390:intron 40.0%
TACGGTACACACGCAAATTA+AGG + chr5_2:67336622-67336641 Msa0783390:intron 40.0%
GCAAAATATGGAAGCTATGG+TGG + chr5_2:67337113-67337132 Msa0783390:CDS 40.0%
AAATATGGAAGCTATGGTGG+GGG + chr5_2:67337116-67337135 Msa0783390:CDS 40.0%
!! AATTATGGTGGTGGTTATCC+TGG + chr5_2:67337158-67337177 Msa0783390:CDS 40.0%
AGCGACAATAATTACCACCA+CGG - chr5_2:67337211-67337230 None:intergenic 40.0%
GGTGGTAATTATTGTCGCTA+TGG + chr5_2:67337212-67337231 Msa0783390:CDS 40.0%
! AGTGTTGTTCTCAGTTTGCA+CGG - chr5_2:67337303-67337322 None:intergenic 40.0%
CGTTGTGTGTTTATCAAGAG+TGG + chr5_2:67337391-67337410 Msa0783390:three_prime_UTR 40.0%
GTACTCCTTTGTATGTGCTT+CGG + chr5_2:67337479-67337498 Msa0783390:three_prime_UTR 40.0%
CATTCTTCTTATCTCCTCAG+AGG + chr5_2:67336116-67336135 Msa0783390:CDS 40.0%
TCTTATCTCCTCAGAGGTTT+CGG + chr5_2:67336122-67336141 Msa0783390:CDS 40.0%
!!! CTCACCTTTTTGGGTATTAG+AGG - chr5_2:67336169-67336188 None:intergenic 40.0%
TAACGGTGGAGTCATTATGA+AGG - chr5_2:67336306-67336325 None:intergenic 40.0%
TGAAATAGAGCTTCTAACGG+TGG - chr5_2:67336320-67336339 None:intergenic 40.0%
CCGTTAGAAGCTCTATTTCA+TGG + chr5_2:67336320-67336339 Msa0783390:intron 40.0%
TACGGTACACACGCAAATTA+AGG + chr5_2:67336622-67336641 Msa0783390:intron 40.0%
GCAAAATATGGAAGCTATGG+TGG + chr5_2:67337113-67337132 Msa0783390:CDS 40.0%
AAATATGGAAGCTATGGTGG+GGG + chr5_2:67337116-67337135 Msa0783390:CDS 40.0%
!! AATTATGGTGGTGGTTATCC+TGG + chr5_2:67337158-67337177 Msa0783390:CDS 40.0%
AGCGACAATAATTACCACCA+CGG - chr5_2:67337211-67337230 None:intergenic 40.0%
GGTGGTAATTATTGTCGCTA+TGG + chr5_2:67337212-67337231 Msa0783390:CDS 40.0%
! AGTGTTGTTCTCAGTTTGCA+CGG - chr5_2:67337303-67337322 None:intergenic 40.0%
CGTTGTGTGTTTATCAAGAG+TGG + chr5_2:67337391-67337410 Msa0783390:three_prime_UTR 40.0%
GTACTCCTTTGTATGTGCTT+CGG + chr5_2:67337479-67337498 Msa0783390:three_prime_UTR 40.0%
ATTGTGTGTCGTGACCACTT+CGG + chr5_2:67336547-67336566 Msa0783390:intron 45.0%
AGAGAAAAAGCTTCCCGAAG+TGG - chr5_2:67336564-67336583 None:intergenic 45.0%
CGTACGTAATGTGTTTGCAG+AGG + chr5_2:67337061-67337080 Msa0783390:intron 45.0%
AATTACCACCACGGTTTCCA+GGG - chr5_2:67337202-67337221 None:intergenic 45.0%
TAATTACCACCACGGTTTCC+AGG - chr5_2:67337203-67337222 None:intergenic 45.0%
TATTGTCGCTATGGCTGTTG+CGG + chr5_2:67337221-67337240 Msa0783390:CDS 45.0%
TGTTGCGGTGACAGGTATTA+TGG + chr5_2:67337236-67337255 Msa0783390:CDS 45.0%
GTTGCGGTGACAGGTATTAT+GGG + chr5_2:67337237-67337256 Msa0783390:CDS 45.0%
GGTATTATGGGAGTTGCAGA+AGG + chr5_2:67337249-67337268 Msa0783390:CDS 45.0%
!! AGAAGGTGTTGCTCATATGC+TGG + chr5_2:67337266-67337285 Msa0783390:CDS 45.0%
ATTGTGTGTCGTGACCACTT+CGG + chr5_2:67336547-67336566 Msa0783390:intron 45.0%
AGAGAAAAAGCTTCCCGAAG+TGG - chr5_2:67336564-67336583 None:intergenic 45.0%
CGTACGTAATGTGTTTGCAG+AGG + chr5_2:67337061-67337080 Msa0783390:intron 45.0%
AATTACCACCACGGTTTCCA+GGG - chr5_2:67337202-67337221 None:intergenic 45.0%
TAATTACCACCACGGTTTCC+AGG - chr5_2:67337203-67337222 None:intergenic 45.0%
TATTGTCGCTATGGCTGTTG+CGG + chr5_2:67337221-67337240 Msa0783390:CDS 45.0%
TGTTGCGGTGACAGGTATTA+TGG + chr5_2:67337236-67337255 Msa0783390:CDS 45.0%
GTTGCGGTGACAGGTATTAT+GGG + chr5_2:67337237-67337256 Msa0783390:CDS 45.0%
GGTATTATGGGAGTTGCAGA+AGG + chr5_2:67337249-67337268 Msa0783390:CDS 45.0%
!! AGAAGGTGTTGCTCATATGC+TGG + chr5_2:67337266-67337285 Msa0783390:CDS 45.0%
!! AGATTAATTATTATAATTAT+AGG + chr5_2:67336891-67336910 Msa0783390:intron 5.0%
!! AGATTAATTATTATAATTAT+AGG + chr5_2:67336891-67336910 Msa0783390:intron 5.0%
CATGTCACCGGCATTTCTGA+AGG - chr5_2:67336256-67336275 None:intergenic 50.0%
TTGTGTGTCGTGACCACTTC+GGG + chr5_2:67336548-67336567 Msa0783390:intron 50.0%
CCATAATTACCACCACGACC+AGG - chr5_2:67337146-67337165 None:intergenic 50.0%
CCTGGTCGTGGTGGTAATTA+TGG + chr5_2:67337143-67337162 Msa0783390:CDS 50.0%
GGTCGTGGTGGTAATTATGG+TGG + chr5_2:67337146-67337165 Msa0783390:CDS 50.0%
CGTGGTGGTAATTATGGTGG+TGG + chr5_2:67337149-67337168 Msa0783390:CDS 50.0%
CATGTCACCGGCATTTCTGA+AGG - chr5_2:67336256-67336275 None:intergenic 50.0%
TTGTGTGTCGTGACCACTTC+GGG + chr5_2:67336548-67336567 Msa0783390:intron 50.0%
CCATAATTACCACCACGACC+AGG - chr5_2:67337146-67337165 None:intergenic 50.0%
CCTGGTCGTGGTGGTAATTA+TGG + chr5_2:67337143-67337162 Msa0783390:CDS 50.0%
GGTCGTGGTGGTAATTATGG+TGG + chr5_2:67337146-67337165 Msa0783390:CDS 50.0%
CGTGGTGGTAATTATGGTGG+TGG + chr5_2:67337149-67337168 Msa0783390:CDS 50.0%
TCTCCTCAGAGGTTTCGGCT+AGG + chr5_2:67336127-67336146 Msa0783390:CDS 55.0%
CTCCTCAGAGGTTTCGGCTA+GGG + chr5_2:67336128-67336147 Msa0783390:CDS 55.0%
GGTTATCCTGGAAACCGTCG+TGG + chr5_2:67337170-67337189 Msa0783390:CDS 55.0%
TATCCTGGAAACCGTCGTGG+TGG + chr5_2:67337173-67337192 Msa0783390:CDS 55.0%
TAACCACCACGACGGTTTCC+AGG - chr5_2:67337179-67337198 None:intergenic 55.0%
TTCCAGGGTAACCACCACGA+CGG - chr5_2:67337187-67337206 None:intergenic 55.0%
TCTCCTCAGAGGTTTCGGCT+AGG + chr5_2:67336127-67336146 Msa0783390:CDS 55.0%
CTCCTCAGAGGTTTCGGCTA+GGG + chr5_2:67336128-67336147 Msa0783390:CDS 55.0%
GGTTATCCTGGAAACCGTCG+TGG + chr5_2:67337170-67337189 Msa0783390:CDS 55.0%
TATCCTGGAAACCGTCGTGG+TGG + chr5_2:67337173-67337192 Msa0783390:CDS 55.0%
TAACCACCACGACGGTTTCC+AGG - chr5_2:67337179-67337198 None:intergenic 55.0%
TTCCAGGGTAACCACCACGA+CGG - chr5_2:67337187-67337206 None:intergenic 55.0%
GTCCCTAGCCGAAACCTCTG+AGG - chr5_2:67336133-67336152 None:intergenic 60.0%
! AGCTATGGTGGGGGCTATCC+TGG + chr5_2:67337125-67337144 Msa0783390:CDS 60.0%
AACCGTCGTGGTGGTTACCC+TGG + chr5_2:67337182-67337201 Msa0783390:CDS 60.0%
GGTGGTTACCCTGGAAACCG+TGG + chr5_2:67337191-67337210 Msa0783390:CDS 60.0%
GGTTACCCTGGAAACCGTGG+TGG + chr5_2:67337194-67337213 Msa0783390:CDS 60.0%
GCTATGGCTGTTGCGGTGAC+AGG + chr5_2:67337228-67337247 Msa0783390:CDS 60.0%
GTCCCTAGCCGAAACCTCTG+AGG - chr5_2:67336133-67336152 None:intergenic 60.0%
! AGCTATGGTGGGGGCTATCC+TGG + chr5_2:67337125-67337144 Msa0783390:CDS 60.0%
AACCGTCGTGGTGGTTACCC+TGG + chr5_2:67337182-67337201 Msa0783390:CDS 60.0%
GGTGGTTACCCTGGAAACCG+TGG + chr5_2:67337191-67337210 Msa0783390:CDS 60.0%
GGTTACCCTGGAAACCGTGG+TGG + chr5_2:67337194-67337213 Msa0783390:CDS 60.0%
GCTATGGCTGTTGCGGTGAC+AGG + chr5_2:67337228-67337247 Msa0783390:CDS 60.0%
GGTGGGGGCTATCCTGGTCG+TGG + chr5_2:67337131-67337150 Msa0783390:CDS 70.0%
GGGGGCTATCCTGGTCGTGG+TGG + chr5_2:67337134-67337153 Msa0783390:CDS 70.0%
GGTGGGGGCTATCCTGGTCG+TGG + chr5_2:67337131-67337150 Msa0783390:CDS 70.0%
GGGGGCTATCCTGGTCGTGG+TGG + chr5_2:67337134-67337153 Msa0783390:CDS 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 67336036 67337690 67336036 ID=Msa0783390;Name=Msa0783390
chr5_2 mRNA 67336036 67337690 67336036 ID=Msa0783390-mRNA-1;Parent=Msa0783390;Name=Msa0783390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=36|1|1|1|1|1|2|359|120
chr5_2 exon 67336036 67336183 67336036 ID=Msa0783390-mRNA-1:exon:20210;Parent=Msa0783390-mRNA-1
chr5_2 exon 67337081 67337690 67337081 ID=Msa0783390-mRNA-1:exon:20211;Parent=Msa0783390-mRNA-1
chr5_2 five_prime_UTR 67336036 67336071 67336036 ID=Msa0783390-mRNA-1:five_prime_utr;Parent=Msa0783390-mRNA-1
chr5_2 CDS 67336072 67336183 67336072 ID=Msa0783390-mRNA-1:cds;Parent=Msa0783390-mRNA-1
chr5_2 CDS 67337081 67337331 67337081 ID=Msa0783390-mRNA-1:cds;Parent=Msa0783390-mRNA-1
chr5_2 three_prime_UTR 67337332 67337690 67337332 ID=Msa0783390-mRNA-1:three_prime_utr;Parent=Msa0783390-mRNA-1
Gene Sequence

>Msa0783390

ATGGATTTGAAAAATGCAATCCTTATCCTAGGTTTATTGGCTATCATTCTTCTTATCTCCTCAGAGGTTTCGGCTAGGGACTTAACTGAGACATCCTCTAATACCCAAAAAGAGGTTGATGATCAAACAAATGAATTAAATGATGCAAAATATGGAAGCTATGGTGGGGGCTATCCTGGTCGTGGTGGTAATTATGGTGGTGGTTATCCTGGAAACCGTCGTGGTGGTTACCCTGGAAACCGTGGTGGTAATTATTGTCGCTATGGCTGTTGCGGTGACAGGTATTATGGGAGTTGCAGAAGGTGTTGCTCATATGCTGGTGAAGCTGTTGCCGTGCAAACTGAGAACAACACTCGTAACTGA
Protein sequence

>Msa0783390

MDLKNAILILGLLAIILLISSEVSARDLTETSSNTQKEVDDQTNELNDAKYGSYGGGYPGRGGNYGGGYPGNRRGGYPGNRGGNYCRYGCCGDRYYGSCRRCCSYAGEAVAVQTENNTRN*