Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0789030 | sp|O22808|LYK5_ARATH | 64.815 | 54 | 18 | 1 | 5 | 57 | 368 | 421 | 4.23e-14 | 68.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0789030 | G7KAT5 | 94.444 | 54 | 3 | 0 | 4 | 57 | 352 | 405 | 3.10e-25 | 107 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0789020 | Msa0789030 | 0.810220 | 1.284402e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0789030 | MtrunA17_Chr5g0403381 | 94.444 | 54 | 3 | 0 | 4 | 57 | 352 | 405 | 5.97e-29 | 107 |
| Msa0789030 | MtrunA17_Chr5g0439334 | 60.377 | 53 | 21 | 0 | 5 | 57 | 352 | 404 | 5.93e-15 | 67.8 |
| Msa0789030 | MtrunA17_Chr3g0119351 | 51.667 | 60 | 28 | 1 | 5 | 63 | 377 | 436 | 3.61e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0789030 | AT2G33580.1 | 64.815 | 54 | 18 | 1 | 5 | 57 | 368 | 421 | 4.30e-15 | 68.6 |
Find 12 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTATCGCGCTGTTATAAA+AGG | 0.195615 | 5_2:-76766986 | Msa0789030:CDS |
| AGTCCAAGCTGTCGGATTAA+AGG | 0.235075 | 5_2:-76767013 | Msa0789030:CDS |
| TTCCAATGTTATTCGCCTTT+CGG | 0.307747 | 5_2:-76766891 | Msa0789030:CDS |
| CGCTGTTATAAAAGGCGATT+TGG | 0.328137 | 5_2:-76766978 | Msa0789030:CDS |
| GTTTATGAGTATGCTGCTAA+TGG | 0.344131 | 5_2:-76766837 | Msa0789030:CDS |
| TTCGGTGTTAGCTTCAATGA+AGG | 0.496557 | 5_2:-76766873 | Msa0789030:CDS |
| CACCGAAAGGCGAATAACAT+TGG | 0.520543 | 5_2:+76766889 | None:intergenic |
| GTTCCTTTAATCCGACAGCT+TGG | 0.522097 | 5_2:+76767010 | None:intergenic |
| GCTGCGATTAAGAAAACAGA+AGG | 0.590208 | 5_2:-76766956 | Msa0789030:CDS |
| TTAGCTTCAATGAAGGACAT+TGG | 0.611365 | 5_2:-76766866 | Msa0789030:CDS |
| TCATTGAAGCTAACACCGAA+AGG | 0.620422 | 5_2:+76766876 | None:intergenic |
| TAGCAGCATACTCATAAACA+AGG | 0.682033 | 5_2:+76766841 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGTCTGAGATTATATG+TGG | - | chr5_2:76766833-76766852 | Msa0789030:CDS | 25.0% |
| !! | TTTCAAAGTGTATGATTTTG+AGG | - | chr5_2:76766868-76766887 | Msa0789030:CDS | 25.0% |
| !!! | GTATGATTTTGAGGAAATTA+AGG | - | chr5_2:76766877-76766896 | Msa0789030:CDS | 25.0% |
| !! | ATAATTTTAGTCCAAGCTGT+CGG | - | chr5_2:76766909-76766928 | Msa0789030:CDS | 30.0% |
| GTTTATCGCGCTGTTATAAA+AGG | - | chr5_2:76766944-76766963 | Msa0789030:CDS | 35.0% | |
| TTCCAATGTTATTCGCCTTT+CGG | - | chr5_2:76767039-76767058 | Msa0789030:intron | 35.0% | |
| TTAGCTTCAATGAAGGACAT+TGG | - | chr5_2:76767064-76767083 | Msa0789030:intron | 35.0% | |
| TAGCAGCATACTCATAAACA+AGG | + | chr5_2:76767092-76767111 | None:intergenic | 35.0% | |
| GTTTATGAGTATGCTGCTAA+TGG | - | chr5_2:76767093-76767112 | Msa0789030:intron | 35.0% | |
| CGCTGTTATAAAAGGCGATT+TGG | - | chr5_2:76766952-76766971 | Msa0789030:CDS | 40.0% | |
| GCTGCGATTAAGAAAACAGA+AGG | - | chr5_2:76766974-76766993 | Msa0789030:CDS | 40.0% | |
| TCATTGAAGCTAACACCGAA+AGG | + | chr5_2:76767057-76767076 | None:intergenic | 40.0% | |
| !! | TTCGGTGTTAGCTTCAATGA+AGG | - | chr5_2:76767057-76767076 | Msa0789030:intron | 40.0% |
| AGTCCAAGCTGTCGGATTAA+AGG | - | chr5_2:76766917-76766936 | Msa0789030:CDS | 45.0% | |
| GTTCCTTTAATCCGACAGCT+TGG | + | chr5_2:76766923-76766942 | None:intergenic | 45.0% | |
| CACCGAAAGGCGAATAACAT+TGG | + | chr5_2:76767044-76767063 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 76766829 | 76767123 | 76766829 | ID=Msa0789030;Name=Msa0789030 |
| chr5_2 | mRNA | 76766829 | 76767123 | 76766829 | ID=Msa0789030-mRNA-1;Parent=Msa0789030;Name=Msa0789030-mRNA-1;_AED=0.44;_eAED=0.56;_QI=0|0|0|1|1|1|2|0|77 |
| chr5_2 | exon | 76767115 | 76767123 | 76767115 | ID=Msa0789030-mRNA-1:exon:23357;Parent=Msa0789030-mRNA-1 |
| chr5_2 | exon | 76766829 | 76767053 | 76766829 | ID=Msa0789030-mRNA-1:exon:23356;Parent=Msa0789030-mRNA-1 |
| chr5_2 | CDS | 76767115 | 76767123 | 76767115 | ID=Msa0789030-mRNA-1:cds;Parent=Msa0789030-mRNA-1 |
| chr5_2 | CDS | 76766829 | 76767053 | 76766829 | ID=Msa0789030-mRNA-1:cds;Parent=Msa0789030-mRNA-1 |
| Gene Sequence |
| Protein sequence |
