AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0797510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0797510 MtrunA17_Chr5g0400761 94.656 131 6 1 1 130 1 131 8.29e-91 258
Msa0797510 MtrunA17_Chr4g0054461 65.625 96 33 0 35 130 7 102 2.09e-44 140
Msa0797510 MtrunA17_Chr3g0140691 59.155 71 26 2 61 130 30 98 3.09e-23 86.7
Msa0797510 MtrunA17_Chr3g0123851 39.474 76 42 2 59 130 46 121 4.39e-13 61.2
Msa0797510 MtrunA17_Chr4g0063871 32.000 125 68 4 6 130 7 114 1.02e-11 57.8
Msa0797510 MtrunA17_Chr8g0381891 39.474 76 42 2 59 130 48 123 1.05e-11 57.8
Msa0797510 MtrunA17_Chr2g0330071 30.216 139 65 5 11 130 3 128 6.49e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0797510 AT4G37810.1 47.706 109 49 3 30 130 20 128 9.62e-26 94.4
Msa0797510 AT5G10310.2 40.244 82 35 3 59 130 51 128 3.07e-11 57.0
Msa0797510 AT5G10310.1 40.244 82 35 3 59 130 45 122 4.10e-11 56.6

Find 39 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCACCTTGTATCACATTT+TGG 0.211299 5_3:+9696033 None:intergenic
GAATATCATCATGTCATTTA+TGG 0.231378 5_3:-9696561 Msa0797510:CDS
CATCTAAATGAGCTACATTA+TGG 0.294334 5_3:+9695827 None:intergenic
GTCATTGCTCGAGGATTTGC+TGG 0.332737 5_3:+9695979 None:intergenic
CATTTGCATTTCCAACTCAT+AGG 0.395492 5_3:+9695886 None:intergenic
TAGAGAAAGGTGTTCAAGAA+AGG 0.412468 5_3:+9696609 None:intergenic
CCAACTCATAGGTTTATAGT+TGG 0.415908 5_3:+9695897 None:intergenic
AGGGAGAATTCTTTAGAGAA+AGG 0.416869 5_3:+9696596 None:intergenic
TATGAGAAAGAGAAGAGAAA+TGG 0.420044 5_3:+9696518 None:intergenic
TGTTGATTTGTGCAGACAAT+AGG 0.427844 5_3:-9696099 Msa0797510:intron
AGTTTCAAGCTTGACACAAA+AGG 0.454396 5_3:-9696496 Msa0797510:CDS
AGAGAAAGGTGTTCAAGAAA+GGG 0.460515 5_3:+9696610 None:intergenic
TGATATTCATTCCTCATTTG+AGG 0.464024 5_3:+9696576 None:intergenic
TTATGGAACAAATCAAATCA+AGG 0.478334 5_3:+9695844 None:intergenic
GATATTCATTCCTCATTTGA+GGG 0.488619 5_3:+9696577 None:intergenic
GTTTCAAGCTTGACACAAAA+GGG 0.500719 5_3:-9696495 Msa0797510:CDS
ATCTTTCCTGTCATTGCTCG+AGG 0.500929 5_3:+9695970 None:intergenic
GCAACTGCAGACAGAGTTGA+AGG 0.512648 5_3:+9695943 None:intergenic
GTAGCTCATTTAGATGATCA+AGG 0.537745 5_3:-9695820 Msa0797510:three_prime_UTR
GCAAATCCTCGAGCAATGAC+AGG 0.547611 5_3:-9695976 Msa0797510:CDS
GTGATGGTGAGGAAGCAGAT+AGG 0.550422 5_3:-9696066 Msa0797510:CDS
AAAGAATTCTCCCTCAAATG+AGG 0.557496 5_3:-9696587 Msa0797510:exon
CCAACTATAAACCTATGAGT+TGG 0.573239 5_3:-9695897 Msa0797510:CDS
GGCCACCAAAATGTGATACA+AGG 0.578748 5_3:-9696038 Msa0797510:CDS
TGTCTGCAGTTGCTTATGCT+AGG 0.585427 5_3:-9695933 Msa0797510:CDS
TTACCTGGTGAAAACCATGT+TGG 0.587700 5_3:+9696350 None:intergenic
GTGATAACAAGGTGATGGTG+AGG 0.587937 5_3:-9696077 Msa0797510:CDS
TATGGAACAAATCAAATCAA+GGG 0.596622 5_3:+9695845 None:intergenic
CTGCAGTTGCTTATGCTAGG+AGG 0.607524 5_3:-9695930 Msa0797510:CDS
ATGAGTTGGAAATGCAAATG+TGG 0.611058 5_3:-9695883 Msa0797510:CDS
AGAAAGACTTTGAACCAACA+TGG 0.614586 5_3:-9696364 Msa0797510:CDS
CAAAAGGGACTTGTAACTGA+AGG 0.617750 5_3:-9696480 Msa0797510:intron
AATAGGTGATAACAAGGTGA+TGG 0.620627 5_3:-9696082 Msa0797510:CDS
TGAGGAAGCAGATAGGATCA+AGG 0.622434 5_3:-9696059 Msa0797510:CDS
GATACAAGGTGCAGATTCTG+TGG 0.657438 5_3:-9696024 Msa0797510:CDS
GCAGACAATAGGTGATAACA+AGG 0.666495 5_3:-9696088 Msa0797510:intron
CAGATTCTGTGGTCACTGCG+AGG 0.669680 5_3:-9696013 Msa0797510:CDS
TGAGTTGGAAATGCAAATGT+GGG 0.678868 5_3:-9695882 Msa0797510:CDS
CAACTGCAGACAGAGTTGAA+GGG 0.720563 5_3:+9695944 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAGTTATAAGAATT+TGG + chr5_3:9696081-9696100 None:intergenic 10.0%
!! AAAAAAAAGTTATAAGAATT+TGG + chr5_3:9696081-9696100 None:intergenic 10.0%
!!! TTAATTATTTTTCTCATTTG+AGG - chr5_3:9696104-9696123 Msa0797510:intron 15.0%
!!! TTAATTATTTTTCTCATTTG+AGG - chr5_3:9696104-9696123 Msa0797510:intron 15.0%
!! AAATGTAAACAAAATTTACC+TGG + chr5_3:9696036-9696055 None:intergenic 20.0%
!! AAATGTAAACAAAATTTACC+TGG + chr5_3:9696036-9696055 None:intergenic 20.0%
!! GAATATCATCATGTCATTTA+TGG - chr5_3:9695807-9695826 Msa0797510:three_prime_UTR 25.0%
! TTGAAGTAAGAGTTATAAGT+TGG + chr5_3:9695938-9695957 None:intergenic 25.0%
! AAGTTATAAGAATTTGGAAG+AGG + chr5_3:9696075-9696094 None:intergenic 25.0%
! TATGGAACAAATCAAATCAA+GGG + chr5_3:9696526-9696545 None:intergenic 25.0%
! TTATGGAACAAATCAAATCA+AGG + chr5_3:9696527-9696546 None:intergenic 25.0%
!! GAATATCATCATGTCATTTA+TGG - chr5_3:9695807-9695826 Msa0797510:three_prime_UTR 25.0%
! TTGAAGTAAGAGTTATAAGT+TGG + chr5_3:9695938-9695957 None:intergenic 25.0%
! AAGTTATAAGAATTTGGAAG+AGG + chr5_3:9696075-9696094 None:intergenic 25.0%
! TATGGAACAAATCAAATCAA+GGG + chr5_3:9696526-9696545 None:intergenic 25.0%
! TTATGGAACAAATCAAATCA+AGG + chr5_3:9696527-9696546 None:intergenic 25.0%
GATATTCATTCCTCATTTGA+GGG + chr5_3:9695794-9695813 None:intergenic 30.0%
TGATATTCATTCCTCATTTG+AGG + chr5_3:9695795-9695814 None:intergenic 30.0%
TATGAGAAAGAGAAGAGAAA+TGG + chr5_3:9695853-9695872 None:intergenic 30.0%
CTAATTAAGATGAGTGATGT+AGG - chr5_3:9696232-9696251 Msa0797510:intron 30.0%
CATCTAAATGAGCTACATTA+TGG + chr5_3:9696544-9696563 None:intergenic 30.0%
GATATTCATTCCTCATTTGA+GGG + chr5_3:9695794-9695813 None:intergenic 30.0%
TGATATTCATTCCTCATTTG+AGG + chr5_3:9695795-9695814 None:intergenic 30.0%
TATGAGAAAGAGAAGAGAAA+TGG + chr5_3:9695853-9695872 None:intergenic 30.0%
CTAATTAAGATGAGTGATGT+AGG - chr5_3:9696232-9696251 Msa0797510:intron 30.0%
CATCTAAATGAGCTACATTA+TGG + chr5_3:9696544-9696563 None:intergenic 30.0%
!! AGAGAAAGGTGTTCAAGAAA+GGG + chr5_3:9695761-9695780 None:intergenic 35.0%
!! TAGAGAAAGGTGTTCAAGAA+AGG + chr5_3:9695762-9695781 None:intergenic 35.0%
! AGGGAGAATTCTTTAGAGAA+AGG + chr5_3:9695775-9695794 None:intergenic 35.0%
AAAGAATTCTCCCTCAAATG+AGG - chr5_3:9695781-9695800 Msa0797510:three_prime_UTR 35.0%
AGTTTCAAGCTTGACACAAA+AGG - chr5_3:9695872-9695891 Msa0797510:CDS 35.0%
GTTTCAAGCTTGACACAAAA+GGG - chr5_3:9695873-9695892 Msa0797510:CDS 35.0%
AGAAAGACTTTGAACCAACA+TGG - chr5_3:9696004-9696023 Msa0797510:CDS 35.0%
ATAAGAATTTGGAAGAGGCA+GGG + chr5_3:9696070-9696089 None:intergenic 35.0%
TATAAGAATTTGGAAGAGGC+AGG + chr5_3:9696071-9696090 None:intergenic 35.0%
!! TGTTGATTTGTGCAGACAAT+AGG - chr5_3:9696269-9696288 Msa0797510:intron 35.0%
AATAGGTGATAACAAGGTGA+TGG - chr5_3:9696286-9696305 Msa0797510:intron 35.0%
CCAACTCATAGGTTTATAGT+TGG + chr5_3:9696474-9696493 None:intergenic 35.0%
CCAACTATAAACCTATGAGT+TGG - chr5_3:9696471-9696490 Msa0797510:intron 35.0%
CATTTGCATTTCCAACTCAT+AGG + chr5_3:9696485-9696504 None:intergenic 35.0%
! ATGAGTTGGAAATGCAAATG+TGG - chr5_3:9696485-9696504 Msa0797510:CDS 35.0%
! TGAGTTGGAAATGCAAATGT+GGG - chr5_3:9696486-9696505 Msa0797510:CDS 35.0%
GTAGCTCATTTAGATGATCA+AGG - chr5_3:9696548-9696567 Msa0797510:CDS 35.0%
!! AGAGAAAGGTGTTCAAGAAA+GGG + chr5_3:9695761-9695780 None:intergenic 35.0%
!! TAGAGAAAGGTGTTCAAGAA+AGG + chr5_3:9695762-9695781 None:intergenic 35.0%
! AGGGAGAATTCTTTAGAGAA+AGG + chr5_3:9695775-9695794 None:intergenic 35.0%
AAAGAATTCTCCCTCAAATG+AGG - chr5_3:9695781-9695800 Msa0797510:three_prime_UTR 35.0%
AGTTTCAAGCTTGACACAAA+AGG - chr5_3:9695872-9695891 Msa0797510:CDS 35.0%
GTTTCAAGCTTGACACAAAA+GGG - chr5_3:9695873-9695892 Msa0797510:CDS 35.0%
AGAAAGACTTTGAACCAACA+TGG - chr5_3:9696004-9696023 Msa0797510:CDS 35.0%
ATAAGAATTTGGAAGAGGCA+GGG + chr5_3:9696070-9696089 None:intergenic 35.0%
TATAAGAATTTGGAAGAGGC+AGG + chr5_3:9696071-9696090 None:intergenic 35.0%
!! TGTTGATTTGTGCAGACAAT+AGG - chr5_3:9696269-9696288 Msa0797510:intron 35.0%
AATAGGTGATAACAAGGTGA+TGG - chr5_3:9696286-9696305 Msa0797510:intron 35.0%
CCAACTCATAGGTTTATAGT+TGG + chr5_3:9696474-9696493 None:intergenic 35.0%
CCAACTATAAACCTATGAGT+TGG - chr5_3:9696471-9696490 Msa0797510:intron 35.0%
CATTTGCATTTCCAACTCAT+AGG + chr5_3:9696485-9696504 None:intergenic 35.0%
! ATGAGTTGGAAATGCAAATG+TGG - chr5_3:9696485-9696504 Msa0797510:CDS 35.0%
! TGAGTTGGAAATGCAAATGT+GGG - chr5_3:9696486-9696505 Msa0797510:CDS 35.0%
GTAGCTCATTTAGATGATCA+AGG - chr5_3:9696548-9696567 Msa0797510:CDS 35.0%
CAAAAGGGACTTGTAACTGA+AGG - chr5_3:9695888-9695907 Msa0797510:CDS 40.0%
TCTTCTTCTTCTGCTCTTAC+AGG - chr5_3:9695980-9695999 Msa0797510:CDS 40.0%
TTACCTGGTGAAAACCATGT+TGG + chr5_3:9696021-9696040 None:intergenic 40.0%
GTAAGAGTTTGTCAACTCAG+TGG + chr5_3:9696179-9696198 None:intergenic 40.0%
AGACTGAGTAAACGAGTTGT+CGG + chr5_3:9696209-9696228 None:intergenic 40.0%
GCAGACAATAGGTGATAACA+AGG - chr5_3:9696280-9696299 Msa0797510:intron 40.0%
! CACCTTGTATCACATTTTGG+TGG + chr5_3:9696335-9696354 None:intergenic 40.0%
! CTGCACCTTGTATCACATTT+TGG + chr5_3:9696338-9696357 None:intergenic 40.0%
CAAAAGGGACTTGTAACTGA+AGG - chr5_3:9695888-9695907 Msa0797510:CDS 40.0%
TCTTCTTCTTCTGCTCTTAC+AGG - chr5_3:9695980-9695999 Msa0797510:CDS 40.0%
TTACCTGGTGAAAACCATGT+TGG + chr5_3:9696021-9696040 None:intergenic 40.0%
GTAAGAGTTTGTCAACTCAG+TGG + chr5_3:9696179-9696198 None:intergenic 40.0%
AGACTGAGTAAACGAGTTGT+CGG + chr5_3:9696209-9696228 None:intergenic 40.0%
GCAGACAATAGGTGATAACA+AGG - chr5_3:9696280-9696299 Msa0797510:intron 40.0%
! CACCTTGTATCACATTTTGG+TGG + chr5_3:9696335-9696354 None:intergenic 40.0%
! CTGCACCTTGTATCACATTT+TGG + chr5_3:9696338-9696357 None:intergenic 40.0%
! GAACCAACATGGTTTTCACC+AGG - chr5_3:9696015-9696034 Msa0797510:CDS 45.0%
! GTGATAACAAGGTGATGGTG+AGG - chr5_3:9696291-9696310 Msa0797510:intron 45.0%
TGAGGAAGCAGATAGGATCA+AGG - chr5_3:9696309-9696328 Msa0797510:intron 45.0%
GGCCACCAAAATGTGATACA+AGG - chr5_3:9696330-9696349 Msa0797510:intron 45.0%
GATACAAGGTGCAGATTCTG+TGG - chr5_3:9696344-9696363 Msa0797510:intron 45.0%
ATCTTTCCTGTCATTGCTCG+AGG + chr5_3:9696401-9696420 None:intergenic 45.0%
CAACTGCAGACAGAGTTGAA+GGG + chr5_3:9696427-9696446 None:intergenic 45.0%
TGTCTGCAGTTGCTTATGCT+AGG - chr5_3:9696435-9696454 Msa0797510:intron 45.0%
! GAACCAACATGGTTTTCACC+AGG - chr5_3:9696015-9696034 Msa0797510:CDS 45.0%
! GTGATAACAAGGTGATGGTG+AGG - chr5_3:9696291-9696310 Msa0797510:intron 45.0%
TGAGGAAGCAGATAGGATCA+AGG - chr5_3:9696309-9696328 Msa0797510:intron 45.0%
GGCCACCAAAATGTGATACA+AGG - chr5_3:9696330-9696349 Msa0797510:intron 45.0%
GATACAAGGTGCAGATTCTG+TGG - chr5_3:9696344-9696363 Msa0797510:intron 45.0%
ATCTTTCCTGTCATTGCTCG+AGG + chr5_3:9696401-9696420 None:intergenic 45.0%
CAACTGCAGACAGAGTTGAA+GGG + chr5_3:9696427-9696446 None:intergenic 45.0%
TGTCTGCAGTTGCTTATGCT+AGG - chr5_3:9696435-9696454 Msa0797510:intron 45.0%
!! TAAACGAGTTGTCGGCTCAG+TGG + chr5_3:9696201-9696220 None:intergenic 50.0%
! GTGATGGTGAGGAAGCAGAT+AGG - chr5_3:9696302-9696321 Msa0797510:intron 50.0%
GTCATTGCTCGAGGATTTGC+TGG + chr5_3:9696392-9696411 None:intergenic 50.0%
GCAAATCCTCGAGCAATGAC+AGG - chr5_3:9696392-9696411 Msa0797510:intron 50.0%
GCAACTGCAGACAGAGTTGA+AGG + chr5_3:9696428-9696447 None:intergenic 50.0%
CTGCAGTTGCTTATGCTAGG+AGG - chr5_3:9696438-9696457 Msa0797510:intron 50.0%
!! TAAACGAGTTGTCGGCTCAG+TGG + chr5_3:9696201-9696220 None:intergenic 50.0%
! GTGATGGTGAGGAAGCAGAT+AGG - chr5_3:9696302-9696321 Msa0797510:intron 50.0%
GTCATTGCTCGAGGATTTGC+TGG + chr5_3:9696392-9696411 None:intergenic 50.0%
GCAAATCCTCGAGCAATGAC+AGG - chr5_3:9696392-9696411 Msa0797510:intron 50.0%
GCAACTGCAGACAGAGTTGA+AGG + chr5_3:9696428-9696447 None:intergenic 50.0%
CTGCAGTTGCTTATGCTAGG+AGG - chr5_3:9696438-9696457 Msa0797510:intron 50.0%
CAGATTCTGTGGTCACTGCG+AGG - chr5_3:9696355-9696374 Msa0797510:CDS 55.0%
CAGATTCTGTGGTCACTGCG+AGG - chr5_3:9696355-9696374 Msa0797510:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 9695750 9696640 9695750 ID=Msa0797510;Name=Msa0797510
chr5_3 mRNA 9695750 9696640 9695750 ID=Msa0797510-mRNA-1;Parent=Msa0797510;Name=Msa0797510-mRNA-1;_AED=0.01;_eAED=0.01;_QI=48|1|1|1|1|1|3|111|130
chr5_3 exon 9695750 9696106 9695750 ID=Msa0797510-mRNA-1:exon:4175;Parent=Msa0797510-mRNA-1
chr5_3 exon 9696354 9696388 9696354 ID=Msa0797510-mRNA-1:exon:4174;Parent=Msa0797510-mRNA-1
chr5_3 exon 9696481 9696640 9696481 ID=Msa0797510-mRNA-1:exon:4173;Parent=Msa0797510-mRNA-1
chr5_3 five_prime_UTR 9696593 9696640 9696593 ID=Msa0797510-mRNA-1:five_prime_utr;Parent=Msa0797510-mRNA-1
chr5_3 CDS 9696481 9696592 9696481 ID=Msa0797510-mRNA-1:cds;Parent=Msa0797510-mRNA-1
chr5_3 CDS 9696354 9696388 9696354 ID=Msa0797510-mRNA-1:cds;Parent=Msa0797510-mRNA-1
chr5_3 CDS 9695861 9696106 9695861 ID=Msa0797510-mRNA-1:cds;Parent=Msa0797510-mRNA-1
chr5_3 three_prime_UTR 9695750 9695860 9695750 ID=Msa0797510-mRNA-1:three_prime_utr;Parent=Msa0797510-mRNA-1
Gene Sequence

>Msa0797510

ATGAGGAATGAATATCATCATGTCATTTATGGTCAAAGATTCAGTTTCATTACCATTTCTCTTCTCTTTCTCATAGTTTCAAGCTTGACACAAAAGGGACTTGTAACTGAAGGTAGAAAGACTTTGAACCAACATGGTTTTCACCAGACAATAGGTGATAACAAGGTGATGGTGAGGAAGCAGATAGGATCAAGGCCACCAAAATGTGATACAAGGTGCAGATTCTGTGGTCACTGCGAGGCTATTCAAGTTCCAGCAAATCCTCGAGCAATGACAGGAAAGATCAACCCTTCAACTCTGTCTGCAGTTGCTTATGCTAGGAGGCAAGATAATTCCAACTATAAACCTATGAGTTGGAAATGCAAATGTGGGAACATTATTTTCAACCCTTGA

Protein sequence

>Msa0797510

MRNEYHHVIYGQRFSFITISLLFLIVSSLTQKGLVTEGRKTLNQHGFHQTIGDNKVMVRKQIGSRPPKCDTRCRFCGHCEAIQVPANPRAMTGKINPSTLSAVAYARRQDNSNYKPMSWKCKCGNIIFNP*